AlfalfaGEDB | MS.gene058960

M. sativa cultivar XinJiangDaYe / MS.gene058960

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF13912.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058960.t1	XP_013468307.1	93.6	156	10	0	10	165	2	157	2.40E-59	238.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058960.t1	Q9SLD4	52.9	174	63	7	6	166	1	168	2.1e-19	97.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058960.t1	A0A072VJY7	93.6	156	10	0	10	165	2	157	1.7e-59	238.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene058960.t1	TF	C2H2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene058958	MS.gene058960	0.878933	1.88E-69	-1.69E-46
MS.gene058960	MS.gene073835	0.900898	4.62E-78	-1.69E-46
MS.gene058960	MS.gene073836	0.940171	3.68E-100	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058960.t1	MTR_1g067660	94.872	156	8	0	10	165	2	157	8.45e-108	305
MS.gene058960.t1	MTR_1g067650	64.286	168	43	8	6	164	2	161	3.26e-60	184
MS.gene058960.t1	MTR_1g067640	63.690	168	44	8	6	164	1	160	1.74e-59	182
MS.gene058960.t1	MTR_7g100100	60.000	160	54	5	5	163	2	152	3.49e-58	179
MS.gene058960.t1	MTR_2g096540	49.677	155	49	6	6	158	1	128	9.25e-36	121
MS.gene058960.t1	MTR_1g115345	41.451	193	71	6	6	161	1	188	3.72e-33	117
MS.gene058960.t1	MTR_7g023560	52.101	119	49	3	6	120	1	115	4.69e-32	113
MS.gene058960.t1	MTR_2g084315	32.314	229	61	7	12	159	64	279	2.46e-22	90.9
MS.gene058960.t1	MTR_1g090703	34.826	201	65	7	15	151	98	296	2.75e-21	89.0
MS.gene058960.t1	MTR_1g062780	38.095	147	45	3	18	118	80	226	2.15e-19	83.2
MS.gene058960.t1	MTR_1g106730	41.584	101	54	2	19	117	60	157	5.45e-17	75.9
MS.gene058960.t1	MTR_4g057230	31.707	164	56	3	43	159	113	267	5.56e-16	73.9
MS.gene058960.t1	MTR_4g027440	46.429	84	35	2	43	116	220	303	6.76e-16	74.3
MS.gene058960.t1	MTR_0160s0030	38.983	118	54	6	43	157	240	342	2.09e-15	72.8
MS.gene058960.t1	MTR_3g102980	36.620	142	66	5	7	124	37	178	1.50e-13	66.2
MS.gene058960.t1	MTR_1g018420	36.066	122	58	4	41	151	79	191	4.75e-13	65.1
MS.gene058960.t1	MTR_1g077730	34.783	115	34	3	43	117	44	157	1.41e-12	63.2
MS.gene058960.t1	MTR_1g077700	40.217	92	40	3	43	119	60	151	1.68e-12	62.8
MS.gene058960.t1	MTR_4g109340	33.333	126	45	2	43	129	348	473	8.13e-12	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058960.t1	AT2G37430	45.614	171	80	5	6	166	1	168	3.63e-42	139
MS.gene058960.t1	AT3G53600	44.444	171	73	6	6	163	1	162	1.31e-34	119
MS.gene058960.t1	AT2G28710	46.061	165	69	7	6	163	1	152	5.89e-33	115
MS.gene058960.t1	AT5G59820	45.455	154	71	5	16	163	12	158	4.42e-29	105
MS.gene058960.t1	AT2G28200	35.714	196	73	7	15	159	73	266	2.01e-26	101
MS.gene058960.t1	AT3G46090	43.293	164	67	6	14	163	11	162	2.22e-26	98.6
MS.gene058960.t1	AT3G46080	43.902	164	75	7	10	163	4	160	6.41e-26	97.4
MS.gene058960.t1	AT3G46070	40.491	163	79	7	6	156	1	157	1.36e-22	89.0
MS.gene058960.t1	AT5G03510	41.497	147	39	6	15	114	69	215	7.88e-22	89.7
MS.gene058960.t1	AT5G04390	36.471	170	47	5	6	117	89	255	1.17e-20	87.4
MS.gene058960.t1	AT3G10470	44.660	103	26	2	43	114	182	284	1.96e-18	81.6
MS.gene058960.t1	AT2G45120	36.196	163	74	5	18	157	150	305	1.77e-17	78.2
MS.gene058960.t1	AT3G60580	32.530	166	76	4	18	157	126	281	2.29e-16	74.7
MS.gene058960.t1	AT1G02030	48.718	78	39	1	43	119	160	237	1.03e-15	72.8
MS.gene058960.t1	AT1G27730	33.113	151	87	4	14	154	50	196	3.97e-15	70.5
MS.gene058960.t1	AT1G49900	38.318	107	60	3	30	132	735	839	1.25e-14	70.9
MS.gene058960.t1	AT1G49900	44.304	79	40	2	43	117	193	271	6.32e-13	66.2
MS.gene058960.t1	AT3G49930	34.532	139	58	6	4	113	37	171	2.92e-14	68.2
MS.gene058960.t1	AT5G43170	34.483	145	76	5	14	155	45	173	5.02e-13	64.3
MS.gene058960.t1	AT5G56200	44.444	90	29	2	45	113	344	433	9.73e-13	65.5
MS.gene058960.t1	AT4G35280	39.286	112	50	3	15	113	138	244	9.81e-13	64.7
MS.gene058960.t1	AT3G19580	35.433	127	60	6	9	113	61	187	1.19e-12	64.3
MS.gene058960.t1	AT3G19580	35.433	127	60	6	9	113	61	187	1.19e-12	64.3
MS.gene058960.t1	AT5G67450	37.363	91	38	2	42	113	96	186	2.61e-12	63.2
MS.gene058960.t1	AT5G04340	41.176	85	39	2	43	116	89	173	2.28e-11	60.5

Find 41 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTCTTTGGTTTGGTTTCTT+TGG	0.133578	1.2:-51096507	None:intergenic
TTCTCTCTTGGCCAGGCTTT+GGG	0.201073	1.2:+51096719	MS.gene058960:CDS
AAGCTACATGCCCAAACTTT+AGG	0.279584	1.2:+51096647	MS.gene058960:CDS
TTCTTTGGTTTGGTTTCTTT+GGG	0.287528	1.2:-51096506	None:intergenic
ACAGGACTAAAATGTTTCTT+TGG	0.294036	1.2:-51096521	None:intergenic
TATTCAGCAAACCTAAAGTT+TGG	0.298957	1.2:-51096658	None:intergenic
ACTAAAATGTTTCTTTGGTT+TGG	0.313841	1.2:-51096516	None:intergenic
GAGTTTCACGGTATCTAGTT+TGG	0.314780	1.2:-51096624	None:intergenic
ATTCTCTCTTGGCCAGGCTT+TGG	0.331279	1.2:+51096718	MS.gene058960:CDS
TTATTGTTCGGAAATATATA+TGG	0.336579	1.2:+51096899	MS.gene058960:CDS
TGAAAAGATCGAATAGTAAG+AGG	0.344770	1.2:+51096828	MS.gene058960:CDS
AGTTTCACGGTATCTAGTTT+GGG	0.350342	1.2:-51096623	None:intergenic
AATGTAGCACCCATGCGTTT+TGG	0.353287	1.2:-51096684	None:intergenic
ATTCAGCAAACCTAAAGTTT+GGG	0.384341	1.2:-51096657	None:intergenic
AAGAGTGGAGAATATAGATT+TGG	0.395790	1.2:+51096457	MS.gene058960:CDS
CATATGAGAAGGCATAAAAT+TGG	0.416173	1.2:+51096746	MS.gene058960:CDS
AATGACTTGAAGTTATTGTT+CGG	0.424062	1.2:+51096887	MS.gene058960:CDS
TGTGGTAAAGAATTCTCTCT+TGG	0.452482	1.2:+51096707	MS.gene058960:CDS
CTGAATAACCCCAAAACGCA+TGG	0.471935	1.2:+51096674	MS.gene058960:CDS
TTAATTTCGTAGCCATGAAA+AGG	0.477789	1.2:+51096423	MS.gene058960:intron
ACGCATGGGTGCTACATTTG+TGG	0.490183	1.2:+51096689	MS.gene058960:CDS
GGCATGTAGCTTGAGTTTCA+CGG	0.491665	1.2:-51096636	None:intergenic
TCATATGTCCTCCCAAAGCC+TGG	0.497850	1.2:-51096730	None:intergenic
TAGTAAGAGGGTTATGTGCT+TGG	0.501643	1.2:+51096841	MS.gene058960:CDS
TAAAGAATTCTCTCTTGGCC+AGG	0.519657	1.2:+51096712	MS.gene058960:CDS
TTCTCTTCGTTTCAAGCCCT+AGG	0.530440	1.2:+51096575	MS.gene058960:CDS
GAAAAGATCGAATAGTAAGA+GGG	0.539509	1.2:+51096829	MS.gene058960:CDS
TCTCTTGGCCAGGCTTTGGG+AGG	0.541746	1.2:+51096722	MS.gene058960:CDS
GACTAGCTCTATGGCCACCT+AGG	0.545631	1.2:-51096592	None:intergenic
TTTCTTTGGGATGAGAGAGT+AGG	0.548800	1.2:-51096493	None:intergenic
CATAAAATTGGCGTCAACGA+GGG	0.557665	1.2:+51096758	MS.gene058960:CDS
AAGGCAGAGAGACGAAAGAG+TGG	0.561042	1.2:+51096442	MS.gene058960:CDS
ACTAGCTCTATGGCCACCTA+GGG	0.585030	1.2:-51096591	None:intergenic
AACTTCAAGTCATTCTGCAA+AGG	0.606866	1.2:-51096878	None:intergenic
TGAATAACCCCAAAACGCAT+GGG	0.610752	1.2:+51096675	MS.gene058960:CDS
GTGGTGCACTCAAATTCCAC+AGG	0.615607	1.2:-51096539	None:intergenic
CTTTGGGAGGACATATGAGA+AGG	0.625985	1.2:+51096735	MS.gene058960:CDS
TCTTCGTTTCAAGCCCTAGG+TGG	0.635082	1.2:+51096578	MS.gene058960:CDS
CTTGAAACGAAGAGAACTTG+CGG	0.655759	1.2:-51096568	None:intergenic
GCATAAAATTGGCGTCAACG+AGG	0.656146	1.2:+51096757	MS.gene058960:CDS
AGAGAACTTGCGGTTGCATG+TGG	0.663988	1.2:-51096558	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATTATTAAAATGTCAAT+AGG	-	chr1.2:51096374-51096393	None:intergenic	10.0%
!!	AAATAGAAATTGGTATTTAT+AGG	-	chr1.2:51096292-51096311	None:intergenic	15.0%
!!	ATGTTATGAGAAATAGAAAT+TGG	-	chr1.2:51096302-51096321	None:intergenic	20.0%
!!	TTATTGTTCGGAAATATATA+TGG	+	chr1.2:51096899-51096918	MS.gene058960:CDS	20.0%
!!!	TATTCGATCTTTTCAATATT+GGG	-	chr1.2:51096823-51096842	None:intergenic	20.0%
!	AATGACTTGAAGTTATTGTT+CGG	+	chr1.2:51096887-51096906	MS.gene058960:CDS	25.0%
!	ACTAAAATGTTTCTTTGGTT+TGG	-	chr1.2:51096519-51096538	None:intergenic	25.0%
!	TAGGCAAGGTAATTAAATTA+AGG	-	chr1.2:51096355-51096374	None:intergenic	25.0%
!	TATTAAAATGTCAATAGGCA+AGG	-	chr1.2:51096369-51096388	None:intergenic	25.0%
!	TGATGAATGTTTCAAATCTA+AGG	-	chr1.2:51096207-51096226	None:intergenic	25.0%
!	TTCTTTGGTGAAGAAAAATA+AGG	+	chr1.2:51096784-51096803	MS.gene058960:CDS	25.0%
!	TTTCAAATCTAAGGAAACTT+AGG	-	chr1.2:51096198-51096217	None:intergenic	25.0%
!!	CTATTCGATCTTTTCAATAT+TGG	-	chr1.2:51096824-51096843	None:intergenic	25.0%
	AAGAGTGGAGAATATAGATT+TGG	+	chr1.2:51096457-51096476	MS.gene058960:CDS	30.0%
	ACAGGACTAAAATGTTTCTT+TGG	-	chr1.2:51096524-51096543	None:intergenic	30.0%
	ATTCAGCAAACCTAAAGTTT+GGG	-	chr1.2:51096660-51096679	None:intergenic	30.0%
	GAAAAGATCGAATAGTAAGA+GGG	+	chr1.2:51096829-51096848	MS.gene058960:CDS	30.0%
	GTGAAGAAAAATAAGGTTGT+TGG	+	chr1.2:51096791-51096810	MS.gene058960:CDS	30.0%
	TAAGGAAACTTAGGAAGTAA+GGG	-	chr1.2:51096189-51096208	None:intergenic	30.0%
	TATTCAGCAAACCTAAAGTT+TGG	-	chr1.2:51096661-51096680	None:intergenic	30.0%
	TGAAAAGATCGAATAGTAAG+AGG	+	chr1.2:51096828-51096847	MS.gene058960:CDS	30.0%
	TTAATTTCGTAGCCATGAAA+AGG	+	chr1.2:51096423-51096442	MS.gene058960:intron	30.0%
!	CATATGAGAAGGCATAAAAT+TGG	+	chr1.2:51096746-51096765	MS.gene058960:CDS	30.0%
!	CTTTGACTTTAACCAATTGT+TGG	-	chr1.2:51096247-51096266	None:intergenic	30.0%
!!	AAAGAAACATTTTAGTCCTG+TGG	+	chr1.2:51096523-51096542	MS.gene058960:CDS	30.0%
!!	TTCTTTGGTTTGGTTTCTTT+GGG	-	chr1.2:51096509-51096528	None:intergenic	30.0%
!!	TTTCTTTGGTTTGGTTTCTT+TGG	-	chr1.2:51096510-51096529	None:intergenic	30.0%
	AACTTCAAGTCATTCTGCAA+AGG	-	chr1.2:51096881-51096900	None:intergenic	35.0%
	AATTGGTATTTATAGGCCGT+TGG	-	chr1.2:51096285-51096304	None:intergenic	35.0%
	CTAAGGAAACTTAGGAAGTA+AGG	-	chr1.2:51096190-51096209	None:intergenic	35.0%
	TGACTTTAACCAATTGTTGG+AGG	-	chr1.2:51096244-51096263	None:intergenic	35.0%
	TGTGGTAAAGAATTCTCTCT+TGG	+	chr1.2:51096707-51096726	MS.gene058960:CDS	35.0%
!	AGTTTCACGGTATCTAGTTT+GGG	-	chr1.2:51096626-51096645	None:intergenic	35.0%
	AAGCTACATGCCCAAACTTT+AGG	+	chr1.2:51096647-51096666	MS.gene058960:CDS	40.0%
	CAAACAAAGCCTCCAACAAT+TGG	+	chr1.2:51096232-51096251	MS.gene058960:intron	40.0%
	CATAAAATTGGCGTCAACGA+GGG	+	chr1.2:51096758-51096777	MS.gene058960:CDS	40.0%
	CTTGAAACGAAGAGAACTTG+CGG	-	chr1.2:51096571-51096590	None:intergenic	40.0%
	GAGTTTCACGGTATCTAGTT+TGG	-	chr1.2:51096627-51096646	None:intergenic	40.0%
	GTCCTTGAGTTTATCTCCAA+CGG	+	chr1.2:51096266-51096285	MS.gene058960:intron	40.0%
	TAAAGAATTCTCTCTTGGCC+AGG	+	chr1.2:51096712-51096731	MS.gene058960:CDS	40.0%
	TAGTAAGAGGGTTATGTGCT+TGG	+	chr1.2:51096841-51096860	MS.gene058960:CDS	40.0%
	TGAATAACCCCAAAACGCAT+GGG	+	chr1.2:51096675-51096694	MS.gene058960:CDS	40.0%
!!	TTTCTTTGGGATGAGAGAGT+AGG	-	chr1.2:51096496-51096515	None:intergenic	40.0%
!!!	GCTTTTTGTGACTAGCTCTA+TGG	-	chr1.2:51096604-51096623	None:intergenic	40.0%
	CTGAATAACCCCAAAACGCA+TGG	+	chr1.2:51096674-51096693	MS.gene058960:CDS	45.0%
	GCATAAAATTGGCGTCAACG+AGG	+	chr1.2:51096757-51096776	MS.gene058960:CDS	45.0%
	GGCATGTAGCTTGAGTTTCA+CGG	-	chr1.2:51096639-51096658	None:intergenic	45.0%
	TTCTCTTCGTTTCAAGCCCT+AGG	+	chr1.2:51096575-51096594	MS.gene058960:CDS	45.0%
!	AATGTAGCACCCATGCGTTT+TGG	-	chr1.2:51096687-51096706	None:intergenic	45.0%
!	ATGTAGCACCCATGCGTTTT+GGG	-	chr1.2:51096686-51096705	None:intergenic	45.0%
!	CGTCAACGAGGGATTTTCTT+TGG	+	chr1.2:51096769-51096788	MS.gene058960:CDS	45.0%
!	CTTTGGGAGGACATATGAGA+AGG	+	chr1.2:51096735-51096754	MS.gene058960:CDS	45.0%
!	TTCGTCTCTCTGCCTTTTCA+TGG	-	chr1.2:51096438-51096457	None:intergenic	45.0%
	AAGGCAGAGAGACGAAAGAG+TGG	+	chr1.2:51096442-51096461	MS.gene058960:CDS	50.0%
	AGAGAACTTGCGGTTGCATG+TGG	-	chr1.2:51096561-51096580	None:intergenic	50.0%
	ATTCTCTCTTGGCCAGGCTT+TGG	+	chr1.2:51096718-51096737	MS.gene058960:CDS	50.0%
	GGCCGTTGGAGATAAACTCA+AGG	-	chr1.2:51096271-51096290	None:intergenic	50.0%
	GTGGTGCACTCAAATTCCAC+AGG	-	chr1.2:51096542-51096561	None:intergenic	50.0%
	TCATATGTCCTCCCAAAGCC+TGG	-	chr1.2:51096733-51096752	None:intergenic	50.0%
	TCTTCGTTTCAAGCCCTAGG+TGG	+	chr1.2:51096578-51096597	MS.gene058960:CDS	50.0%
	TTCTCTCTTGGCCAGGCTTT+GGG	+	chr1.2:51096719-51096738	MS.gene058960:CDS	50.0%
!	ACGCATGGGTGCTACATTTG+TGG	+	chr1.2:51096689-51096708	MS.gene058960:CDS	50.0%
!	TGTAGCACCCATGCGTTTTG+GGG	-	chr1.2:51096685-51096704	None:intergenic	50.0%
!!	ACTAGCTCTATGGCCACCTA+GGG	-	chr1.2:51096594-51096613	None:intergenic	50.0%
!!	GACTAGCTCTATGGCCACCT+AGG	-	chr1.2:51096595-51096614	None:intergenic	55.0%
!!	TCTCTTGGCCAGGCTTTGGG+AGG	+	chr1.2:51096722-51096741	MS.gene058960:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	51096123	51096928	51096123	ID=MS.gene058960
chr1.2	mRNA	51096123	51096928	51096123	ID=MS.gene058960.t1;Parent=MS.gene058960
chr1.2	exon	51096123	51096135	51096123	ID=MS.gene058960.t1.exon1;Parent=MS.gene058960.t1
chr1.2	CDS	51096123	51096135	51096123	ID=cds.MS.gene058960.t1;Parent=MS.gene058960.t1
chr1.2	exon	51096435	51096928	51096435	ID=MS.gene058960.t1.exon2;Parent=MS.gene058960.t1
chr1.2	CDS	51096435	51096928	51096435	ID=cds.MS.gene058960.t1;Parent=MS.gene058960.t1

Gene Sequence

>MS.gene058960

ATGGTAAAAACAGGTAAAGAGGACAAATATTGATTCATTTCATTATTATAAACTCTTATATAACCCTTACTTCCTAAGTTTCCTTAGATTTGAAACATTCATCAATAAACAAACAAAGCCTCCAACAATTGGTTAAAGTCAAAGTCCTTGAGTTTATCTCCAACGGCCTATAAATACCAATTTCTATTTCTCATAACATCTTCATCACAATATTTTGAGTATTTCATTTCCTTAATTTAATTACCTTGCCTATTGACATTTTAATAATTTTTTTTTTTTGCATAGACAATATTTTTATTCTTAATTTCGTAGCCATGAAAAGGCAGAGAGACGAAAGAGTGGAGAATATAGATTTGGCAAATTGTCTAATCCTACTCTCTCATCCCAAAGAAACCAAACCAAAGAAACATTTTAGTCCTGTGGAATTTGAGTGCACCACATGCAACCGCAAGTTCTCTTCGTTTCAAGCCCTAGGTGGCCATAGAGCTAGTCACAAAAAGCCCAAACTAGATACCGTGAAACTCAAGCTACATGCCCAAACTTTAGGTTTGCTGAATAACCCCAAAACGCATGGGTGCTACATTTGTGGTAAAGAATTCTCTCTTGGCCAGGCTTTGGGAGGACATATGAGAAGGCATAAAATTGGCGTCAACGAGGGATTTTCTTTGGTGAAGAAAAATAAGGTTGTTGGAAAAATCCCAATATTGAAAAGATCGAATAGTAAGAGGGTTATGTGCTTGGACTTGAACTTAACACCTTTGCAGAATGACTTGAAGTTATTGTTCGGAAATATATATGGCACCTGA

Protein sequence

>MS.gene058960.t1

MVKTAMKRQRDERVENIDLANCLILLSHPKETKPKKHFSPVEFECTTCNRKFSSFQALGGHRASHKKPKLDTVKLKLHAQTLGLLNNPKTHGCYICGKEFSLGQALGGHMRRHKIGVNEGFSLVKKNKVVGKIPILKRSNSKRVMCLDLNLTPLQNDLKLLFGNIYGT