Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059219.t1 | XP_024638076.1 | 87.2 | 125 | 16 | 0 | 2 | 126 | 20 | 144 | 4.00E-36 | 161.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059219.t1 | A0A396I3C8 | 87.2 | 125 | 16 | 0 | 2 | 126 | 20 | 144 | 2.9e-36 | 161.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059219 | MS.gene50163 | 0.8017 | 7.96E-49 | -1.69E-46 |
| MS.gene059219 | MS.gene64671 | 0.802164 | 6.39E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 38 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCGATGTAAACATCATCTT+TGG | 0.298416 | 4.1:+73238183 | None:intergenic |
| GCAAAGTTCTAGTTTAAATC+AGG | 0.322392 | 4.1:-73237870 | MS.gene059219:CDS |
| TGGTATTGGTTGCTACATTC+TGG | 0.328741 | 4.1:-73237421 | MS.gene059219:CDS |
| GAAAGGAGTAATAACAATAA+TGG | 0.362988 | 4.1:+73237478 | None:intergenic |
| TCAATGATTGTTGTTTCCTT+TGG | 0.367040 | 4.1:+73237603 | None:intergenic |
| ATAAAGGTTAGTAGATATTC+CGG | 0.374737 | 4.1:-73237963 | MS.gene059219:intron |
| CGTTTGATCATGCTTGAGAA+TGG | 0.397941 | 4.1:+73237769 | None:intergenic |
| CCTTTGGTCATGCTTGAGAA+TGG | 0.405971 | 4.1:+73237619 | None:intergenic |
| TGAGGCCATTGTGATGGTAT+TGG | 0.409319 | 4.1:-73237435 | MS.gene059219:CDS |
| GGAAGATCAATCCAAGTTGC+AGG | 0.431598 | 4.1:-73237725 | MS.gene059219:CDS |
| CCGATGTAAACATCATCTTT+GGG | 0.444317 | 4.1:+73238184 | None:intergenic |
| AGAAAGCATGTTTGAAAACC+TGG | 0.459709 | 4.1:+73237515 | None:intergenic |
| TTTGCACGGATCCTGCCGCT+TGG | 0.501469 | 4.1:+73237564 | None:intergenic |
| TTTGAGAAGAACCTGCAACT+TGG | 0.502572 | 4.1:+73237714 | None:intergenic |
| GCAGAGGACTTCGTAATGTC+CGG | 0.520535 | 4.1:+73237944 | None:intergenic |
| TTCTCCACTACAAGGTGGCA+TGG | 0.521633 | 4.1:+73238144 | None:intergenic |
| ACATCGGTGCACAACTCAAA+CGG | 0.524605 | 4.1:-73238168 | MS.gene059219:CDS |
| TTCTAGTTTAAATCAGGGGA+TGG | 0.525203 | 4.1:-73237864 | MS.gene059219:CDS |
| ATGACTACTATAGACTCTGC+TGG | 0.527721 | 4.1:-73237797 | MS.gene059219:CDS |
| CAAAGTTCTAGTTTAAATCA+GGG | 0.532231 | 4.1:-73237869 | MS.gene059219:CDS |
| GGAAGTTCAATCCAAGCGGC+AGG | 0.538723 | 4.1:-73237575 | MS.gene059219:CDS |
| TCTCCACTACAAGGTGGCAT+GGG | 0.539275 | 4.1:+73238145 | None:intergenic |
| CACTGTAATCTTCATTTGTG+AGG | 0.549757 | 4.1:-73237453 | MS.gene059219:CDS |
| CGCTTGGATTGAACTTCCAG+CGG | 0.555790 | 4.1:+73237580 | None:intergenic |
| CATTTGTGAGGCCATTGTGA+TGG | 0.559546 | 4.1:-73237441 | MS.gene059219:CDS |
| AAACAACAATCATTGACAGC+TGG | 0.563591 | 4.1:-73237746 | MS.gene059219:CDS |
| CGGCCCATGCCACCTTGTAG+TGG | 0.564484 | 4.1:-73238148 | MS.gene059219:CDS |
| ATGAAGATTACAGTGATGAA+AGG | 0.577087 | 4.1:+73237461 | None:intergenic |
| CATGTTAATGTGAACAATGA+TGG | 0.596679 | 4.1:-73238385 | MS.gene059219:intron |
| AAACAACAATCATTGACCGC+TGG | 0.601224 | 4.1:-73237596 | MS.gene059219:CDS |
| AAAGTTCTAGTTTAAATCAG+GGG | 0.604848 | 4.1:-73237868 | MS.gene059219:CDS |
| TGACTACTATAGACTCTGCT+GGG | 0.622977 | 4.1:-73237796 | MS.gene059219:CDS |
| CCCAAAGATGATGTTTACAT+CGG | 0.630117 | 4.1:-73238184 | MS.gene059219:CDS |
| CGCTGGAAGTTCAATCCAAG+CGG | 0.630144 | 4.1:-73237579 | MS.gene059219:CDS |
| ACTTACGGTTCTCCACTACA+AGG | 0.637620 | 4.1:+73238136 | None:intergenic |
| CCATTCTCAAGCATGACCAA+AGG | 0.649776 | 4.1:-73237619 | MS.gene059219:CDS |
| AGCAACCAATACCATCACAA+TGG | 0.720464 | 4.1:+73237430 | None:intergenic |
| TACGGTTCTCCACTACAAGG+TGG | 0.728989 | 4.1:+73238139 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATACATATATTAAATCAC+CGG | + | chr4.1:73237508-73237527 | None:intergenic | 15.0% |
| !!! | TTATACTTTTCAATTGATAA+AGG | - | chr4.1:73237833-73237852 | MS.gene059219:CDS | 15.0% |
| !! | AAAACTGAATTATCAAAAGA+AGG | - | chr4.1:73237734-73237753 | MS.gene059219:CDS | 20.0% |
| !! | AAGAATAATGTATATACTAG+AGG | + | chr4.1:73237564-73237583 | None:intergenic | 20.0% |
| !! | TAAACATAATGTGTTCTAAT+AGG | + | chr4.1:73237781-73237800 | None:intergenic | 20.0% |
| ! | ATAAAGGTTAGTAGATATTC+CGG | - | chr4.1:73237849-73237868 | MS.gene059219:CDS | 25.0% |
| ! | GAAAGGAGTAATAACAATAA+TGG | + | chr4.1:73238337-73238356 | None:intergenic | 25.0% |
| !! | CTTTTTATGTTGTATTTGCA+CGG | + | chr4.1:73238265-73238284 | None:intergenic | 25.0% |
| !!! | AAAGTTCTAGTTTAAATCAG+GGG | - | chr4.1:73237944-73237963 | MS.gene059219:CDS | 25.0% |
| !!! | CAAAGTTCTAGTTTAAATCA+GGG | - | chr4.1:73237943-73237962 | MS.gene059219:CDS | 25.0% |
| !!! | TTGTTGAAAGTTTGTTTTTC+CGG | - | chr4.1:73237486-73237505 | MS.gene059219:CDS | 25.0% |
| !!! | TTTTGATAATTCAGTTTTGC+AGG | + | chr4.1:73237732-73237751 | None:intergenic | 25.0% |
| AACATAAAAAGCAAGATCAC+AGG | - | chr4.1:73238274-73238293 | MS.gene059219:intron | 30.0% | |
| ATGAAGATTACAGTGATGAA+AGG | + | chr4.1:73238354-73238373 | None:intergenic | 30.0% | |
| ATGTGTTCTAATAGGTGAAA+AGG | + | chr4.1:73237773-73237792 | None:intergenic | 30.0% | |
| CATGTTAATGTGAACAATGA+TGG | - | chr4.1:73237427-73237446 | MS.gene059219:CDS | 30.0% | |
| GAACCAAGAAATGAAACTTA+CGG | + | chr4.1:73237694-73237713 | None:intergenic | 30.0% | |
| TAAGTTTCATTTCTTGGTTC+AGG | - | chr4.1:73237694-73237713 | MS.gene059219:CDS | 30.0% | |
| !! | GCAAAGTTCTAGTTTAAATC+AGG | - | chr4.1:73237942-73237961 | MS.gene059219:CDS | 30.0% |
| !! | TCAATGATTGTTGTTTCCTT+TGG | + | chr4.1:73238212-73238231 | None:intergenic | 30.0% |
| !!! | ATTTTGATTCAATTCAGCAG+AGG | + | chr4.1:73237887-73237906 | None:intergenic | 30.0% |
| AAACAACAATCATTGACAGC+TGG | - | chr4.1:73238066-73238085 | MS.gene059219:intron | 35.0% | |
| AAGAAAGAGAGAAACAGTAG+TGG | + | chr4.1:73237539-73237558 | None:intergenic | 35.0% | |
| ACCGATGTAAACATCATCTT+TGG | + | chr4.1:73237632-73237651 | None:intergenic | 35.0% | |
| CACTGTAATCTTCATTTGTG+AGG | - | chr4.1:73238359-73238378 | MS.gene059219:intron | 35.0% | |
| CCCAAAGATGATGTTTACAT+CGG | - | chr4.1:73237628-73237647 | MS.gene059219:CDS | 35.0% | |
| CCGATGTAAACATCATCTTT+GGG | + | chr4.1:73237631-73237650 | None:intergenic | 35.0% | |
| GAACCGTAAGTTTCATTTCT+TGG | - | chr4.1:73237688-73237707 | MS.gene059219:CDS | 35.0% | |
| ! | ACTACTGTTTCTCTCTTTCT+TGG | - | chr4.1:73237538-73237557 | MS.gene059219:CDS | 35.0% |
| ! | AGAAAGCATGTTTGAAAACC+TGG | + | chr4.1:73238300-73238319 | None:intergenic | 35.0% |
| !! | TTCTAGTTTAAATCAGGGGA+TGG | - | chr4.1:73237948-73237967 | MS.gene059219:CDS | 35.0% |
| AAACAACAATCATTGACCGC+TGG | - | chr4.1:73238216-73238235 | MS.gene059219:intron | 40.0% | |
| AGCAACCAATACCATCACAA+TGG | + | chr4.1:73238385-73238404 | None:intergenic | 40.0% | |
| ATGACTACTATAGACTCTGC+TGG | - | chr4.1:73238015-73238034 | MS.gene059219:intron | 40.0% | |
| CGTTTGATCATGCTTGAGAA+TGG | + | chr4.1:73238046-73238065 | None:intergenic | 40.0% | |
| TGACTACTATAGACTCTGCT+GGG | - | chr4.1:73238016-73238035 | MS.gene059219:intron | 40.0% | |
| TTTGAGAAGAACCTGCAACT+TGG | + | chr4.1:73238101-73238120 | None:intergenic | 40.0% | |
| !! | TGGTATTGGTTGCTACATTC+TGG | - | chr4.1:73238391-73238410 | MS.gene059219:CDS | 40.0% |
| AAAAAGCAAGATCACAGGCC+AGG | - | chr4.1:73238279-73238298 | MS.gene059219:intron | 45.0% | |
| ACATCGGTGCACAACTCAAA+CGG | - | chr4.1:73237644-73237663 | MS.gene059219:CDS | 45.0% | |
| ACTTACGGTTCTCCACTACA+AGG | + | chr4.1:73237679-73237698 | None:intergenic | 45.0% | |
| CATTTGTGAGGCCATTGTGA+TGG | - | chr4.1:73238371-73238390 | MS.gene059219:intron | 45.0% | |
| CCATTCTCAAGCATGACCAA+AGG | - | chr4.1:73238193-73238212 | MS.gene059219:CDS | 45.0% | |
| CCTTTGGTCATGCTTGAGAA+TGG | + | chr4.1:73238196-73238215 | None:intergenic | 45.0% | |
| GGAAGATCAATCCAAGTTGC+AGG | - | chr4.1:73238087-73238106 | MS.gene059219:intron | 45.0% | |
| TGAGGCCATTGTGATGGTAT+TGG | - | chr4.1:73238377-73238396 | MS.gene059219:intron | 45.0% | |
| CGCTGGAAGTTCAATCCAAG+CGG | - | chr4.1:73238233-73238252 | MS.gene059219:intron | 50.0% | |
| CGCTTGGATTGAACTTCCAG+CGG | + | chr4.1:73238235-73238254 | None:intergenic | 50.0% | |
| GCAGAGGACTTCGTAATGTC+CGG | + | chr4.1:73237871-73237890 | None:intergenic | 50.0% | |
| TACGGTTCTCCACTACAAGG+TGG | + | chr4.1:73237676-73237695 | None:intergenic | 50.0% | |
| TCTCCACTACAAGGTGGCAT+GGG | + | chr4.1:73237670-73237689 | None:intergenic | 50.0% | |
| TTCTCCACTACAAGGTGGCA+TGG | + | chr4.1:73237671-73237690 | None:intergenic | 50.0% | |
| GGAAGTTCAATCCAAGCGGC+AGG | - | chr4.1:73238237-73238256 | MS.gene059219:intron | 55.0% | |
| TTTGCACGGATCCTGCCGCT+TGG | + | chr4.1:73238251-73238270 | None:intergenic | 60.0% | |
| CGGCCCATGCCACCTTGTAG+TGG | - | chr4.1:73237664-73237683 | MS.gene059219:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 73237394 | 73238440 | 73237394 | ID=MS.gene059219 |
| chr4.1 | mRNA | 73237394 | 73238440 | 73237394 | ID=MS.gene059219.t1;Parent=MS.gene059219 |
| chr4.1 | exon | 73238386 | 73238440 | 73238386 | ID=MS.gene059219.t1.exon1;Parent=MS.gene059219.t1 |
| chr4.1 | CDS | 73238386 | 73238440 | 73238386 | ID=cds.MS.gene059219.t1;Parent=MS.gene059219.t1 |
| chr4.1 | exon | 73238142 | 73238217 | 73238142 | ID=MS.gene059219.t1.exon2;Parent=MS.gene059219.t1 |
| chr4.1 | CDS | 73238142 | 73238217 | 73238142 | ID=cds.MS.gene059219.t1;Parent=MS.gene059219.t1 |
| chr4.1 | exon | 73237394 | 73237979 | 73237394 | ID=MS.gene059219.t1.exon3;Parent=MS.gene059219.t1 |
| chr4.1 | CDS | 73237394 | 73237979 | 73237394 | ID=cds.MS.gene059219.t1;Parent=MS.gene059219.t1 |
| Gene Sequence |
| Protein sequence |
