Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060308.t1 | XP_013470028.1 | 98.2 | 224 | 4 | 0 | 1 | 224 | 1 | 224 | 2.70E-127 | 464.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060308.t1 | C0HJG8 | 55.0 | 60 | 11 | 1 | 162 | 221 | 17 | 60 | 3.0e-10 | 67.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060308.t1 | I3SK49 | 98.2 | 224 | 4 | 0 | 1 | 224 | 1 | 224 | 2.0e-127 | 464.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054093 | MS.gene060308 | 0.8098 | 1.58E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060308.t1 | MTR_1g107215 | 98.214 | 224 | 4 | 0 | 1 | 224 | 1 | 224 | 1.34e-167 | 461 |
| MS.gene060308.t1 | MTR_1g107225 | 49.519 | 208 | 94 | 1 | 15 | 222 | 20 | 216 | 3.99e-74 | 224 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060308.t1 | AT2G15220 | 64.865 | 222 | 77 | 1 | 1 | 221 | 3 | 224 | 2.09e-109 | 313 |
| MS.gene060308.t1 | AT2G15130 | 58.108 | 222 | 92 | 1 | 1 | 221 | 3 | 224 | 3.10e-96 | 280 |
| MS.gene060308.t1 | AT2G15130 | 65.035 | 143 | 50 | 0 | 79 | 221 | 1 | 143 | 3.32e-65 | 198 |
| MS.gene060308.t1 | AT2G15170 | 41.237 | 97 | 54 | 2 | 2 | 95 | 4 | 100 | 2.33e-23 | 90.5 |
Find 47 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAATTCTTTGTTCAACTTT+TGG | 0.208442 | 1.2:+79829390 | MS.gene060308:CDS |
| GATTTGAGAAAGGAGATTAC+AGG | 0.374014 | 1.2:+79829112 | MS.gene060308:CDS |
| TCAGTTGTGGACAGAGTATA+AGG | 0.379326 | 1.2:+79829426 | MS.gene060308:CDS |
| CTAATTATGCACCTAGTCAT+TGG | 0.392540 | 1.2:+79829236 | MS.gene060308:CDS |
| TGTCGGCAGGGTTGGTCTGT+TGG | 0.401432 | 1.2:-79828979 | None:intergenic |
| ATTCCGCGACACAATTCATA+TGG | 0.401613 | 1.2:+79828948 | MS.gene060308:CDS |
| ACAGAGTATAAGGCAAAGTA+TGG | 0.408063 | 1.2:+79829436 | MS.gene060308:CDS |
| TCCCACTTATTTCCTTGCCC+TGG | 0.425274 | 1.2:-79829265 | None:intergenic |
| GTACGTTTCCGTGATCAAAT+AGG | 0.437288 | 1.2:+79828899 | MS.gene060308:CDS |
| TAGTTTAAGAAGTGGGTTTG+TGG | 0.455276 | 1.2:+79829330 | MS.gene060308:CDS |
| CCTGGTTTCACCCAATGACT+AGG | 0.458525 | 1.2:-79829247 | None:intergenic |
| TGGAGTAGAGAGGGCATTGT+TGG | 0.474364 | 1.2:-79828405 | None:intergenic |
| TTGAATAAGTTGATGAGGAA+TGG | 0.487887 | 1.2:+79829358 | MS.gene060308:CDS |
| TAATTATGCACCTAGTCATT+GGG | 0.492399 | 1.2:+79829237 | MS.gene060308:CDS |
| ATTGTGATAGTTTAAGAAGT+GGG | 0.492465 | 1.2:+79829323 | MS.gene060308:CDS |
| TCAAAAGGTGAGTTTGTTTG+TGG | 0.492861 | 1.2:+79829012 | MS.gene060308:CDS |
| GCAAGATATGTTAATAGCTA+TGG | 0.493653 | 1.2:+79829085 | MS.gene060308:CDS |
| CCTAGTCATTGGGTGAAACC+AGG | 0.495577 | 1.2:+79829247 | MS.gene060308:CDS |
| GCTAATGGTGGATTAATTGA+AGG | 0.501572 | 1.2:+79829187 | MS.gene060308:CDS |
| AACAATGCCCTCTCTACTCC+AGG | 0.540173 | 1.2:+79828407 | MS.gene060308:CDS |
| AATGGTAATGGTCAAGCTAA+TGG | 0.545203 | 1.2:+79829172 | MS.gene060308:CDS |
| CTATGGTGGTGATTTGAGAA+AGG | 0.549048 | 1.2:+79829102 | MS.gene060308:CDS |
| GTACTCCTTGCTATAATACA+TGG | 0.553308 | 1.2:+79828356 | MS.gene060308:CDS |
| GGAAGACTGTTAATCAGTTG+TGG | 0.562250 | 1.2:+79829413 | MS.gene060308:CDS |
| ATCATGAAATGACACATGTA+TGG | 0.567977 | 1.2:+79829143 | MS.gene060308:CDS |
| GAATTCCATGTATTATAGCA+AGG | 0.581020 | 1.2:-79828361 | None:intergenic |
| AACCAGGGCAAGGAAATAAG+TGG | 0.582595 | 1.2:+79829263 | MS.gene060308:CDS |
| CAGAGTTGAATAAGTTGATG+AGG | 0.590170 | 1.2:+79829353 | MS.gene060308:CDS |
| ATTGTGCTCCTATTTGATCA+CGG | 0.594837 | 1.2:-79828907 | None:intergenic |
| AAATGACACATGTATGGCAG+TGG | 0.595561 | 1.2:+79829149 | MS.gene060308:CDS |
| TATTGTGATAGTTTAAGAAG+TGG | 0.595855 | 1.2:+79829322 | MS.gene060308:CDS |
| GAGTTTGTTTGTGGACGACA+TGG | 0.595884 | 1.2:+79829021 | MS.gene060308:CDS |
| ATTTGAGAAAGGAGATTACA+GGG | 0.596291 | 1.2:+79829113 | MS.gene060308:CDS |
| AGTAATACCTGGAGTAGAGA+GGG | 0.597095 | 1.2:-79828414 | None:intergenic |
| GTATGGCAGTGGAATGGTAA+TGG | 0.605899 | 1.2:+79829160 | MS.gene060308:CDS |
| TAGTAATACCTGGAGTAGAG+AGG | 0.616886 | 1.2:-79828415 | None:intergenic |
| GGTAATGGTCAAGCTAATGG+TGG | 0.618451 | 1.2:+79829175 | MS.gene060308:CDS |
| GAGCAGTAACATCATAACCT+TGG | 0.634171 | 1.2:-79829288 | None:intergenic |
| CTAGTCATTGGGTGAAACCA+GGG | 0.635998 | 1.2:+79829248 | MS.gene060308:CDS |
| ACCAGGGCAAGGAAATAAGT+GGG | 0.637731 | 1.2:+79829264 | MS.gene060308:CDS |
| CATTGGGTGAAACCAGGGCA+AGG | 0.644436 | 1.2:+79829253 | MS.gene060308:CDS |
| TTTCCATATGAATTGTGTCG+CGG | 0.650444 | 1.2:-79828951 | None:intergenic |
| TTGTTTGTGGACGACATGGA+TGG | 0.660046 | 1.2:+79829025 | MS.gene060308:CDS |
| CAAGGAAATAAGTGGGACCA+AGG | 0.660451 | 1.2:+79829271 | MS.gene060308:CDS |
| ACACATGTATGGCAGTGGAA+TGG | 0.661405 | 1.2:+79829154 | MS.gene060308:CDS |
| TTTGAGAAAGGAGATTACAG+GGG | 0.665077 | 1.2:+79829114 | MS.gene060308:CDS |
| AGATATGTTAATAGCTATGG+TGG | 0.666292 | 1.2:+79829088 | MS.gene060308:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTCTTATTTATTTATTTTTT+TGG | - | chr1.2:79828499-79828518 | None:intergenic | 10.0% |
| !!! | TTTTTTTGCTGTATTATTAA+GGG | - | chr1.2:79828680-79828699 | None:intergenic | 15.0% |
| !!! | TTTTTTTTGCTGTATTATTA+AGG | - | chr1.2:79828681-79828700 | None:intergenic | 15.0% |
| !! | AAAACCTACTTAATTACTAA+CGG | + | chr1.2:79828648-79828667 | MS.gene060308:intron | 20.0% |
| !! | AAAACTCTCAATAACATTAA+TGG | + | chr1.2:79828697-79828716 | MS.gene060308:intron | 20.0% |
| !! | AATTAACTTTATATGGTCAA+TGG | + | chr1.2:79828547-79828566 | MS.gene060308:intron | 20.0% |
| !! | ATTCAAAATTGCATTATTGA+CGG | + | chr1.2:79828621-79828640 | MS.gene060308:intron | 20.0% |
| !! | TCAGAAAATTACATTATTGA+TGG | + | chr1.2:79828740-79828759 | MS.gene060308:intron | 20.0% |
| !!! | AATATTTTTCATCTAATACC+AGG | + | chr1.2:79828872-79828891 | MS.gene060308:intron | 20.0% |
| !!! | GTAATTTTCTGAATTATTGA+GGG | - | chr1.2:79828732-79828751 | None:intergenic | 20.0% |
| !!! | TGTAATTTTCTGAATTATTG+AGG | - | chr1.2:79828733-79828752 | None:intergenic | 20.0% |
| ! | ATTGTGATAGTTTAAGAAGT+GGG | + | chr1.2:79829323-79829342 | MS.gene060308:CDS | 25.0% |
| ! | CCAATAATACATATTAGTGA+GGG | + | chr1.2:79828772-79828791 | MS.gene060308:intron | 25.0% |
| ! | CTATCAAAAATAGTAATACC+TGG | - | chr1.2:79828428-79828447 | None:intergenic | 25.0% |
| ! | GAAGCACAATTAACTTTATA+TGG | + | chr1.2:79828540-79828559 | MS.gene060308:intron | 25.0% |
| ! | TAAACCGTTAGTAATTAAGT+AGG | - | chr1.2:79828655-79828674 | None:intergenic | 25.0% |
| ! | TATTGTGATAGTTTAAGAAG+TGG | + | chr1.2:79829322-79829341 | MS.gene060308:CDS | 25.0% |
| ! | TCACTAATATGTATTATTGG+CGG | - | chr1.2:79828772-79828791 | None:intergenic | 25.0% |
| !! | AATTCTTTGTTCAACTTTTG+GGG | + | chr1.2:79829392-79829411 | MS.gene060308:CDS | 25.0% |
| !! | ATAATGCAATTTTGAATCTG+TGG | - | chr1.2:79828618-79828637 | None:intergenic | 25.0% |
| !! | CAATTCTTTGTTCAACTTTT+GGG | + | chr1.2:79829391-79829410 | MS.gene060308:CDS | 25.0% |
| !! | TAATGCAATTTTGAATCTGT+GGG | - | chr1.2:79828617-79828636 | None:intergenic | 25.0% |
| !! | TCAATTCTTTGTTCAACTTT+TGG | + | chr1.2:79829390-79829409 | MS.gene060308:CDS | 25.0% |
| !!! | AGAATTTTAGTTTTTACCTG+TGG | - | chr1.2:79828825-79828844 | None:intergenic | 25.0% |
| !!! | CTTTTGAACATTTTTTCTGT+CGG | - | chr1.2:79828999-79829018 | None:intergenic | 25.0% |
| ACTCTCAATAACATTAATGG+TGG | + | chr1.2:79828700-79828719 | MS.gene060308:intron | 30.0% | |
| AGATATGTTAATAGCTATGG+TGG | + | chr1.2:79829088-79829107 | MS.gene060308:CDS | 30.0% | |
| ATCATGAAATGACACATGTA+TGG | + | chr1.2:79829143-79829162 | MS.gene060308:CDS | 30.0% | |
| ATTTGAGAAAGGAGATTACA+GGG | + | chr1.2:79829113-79829132 | MS.gene060308:CDS | 30.0% | |
| CCCTCACTAATATGTATTAT+TGG | - | chr1.2:79828775-79828794 | None:intergenic | 30.0% | |
| CGACAGAAAAAATGTTCAAA+AGG | + | chr1.2:79828997-79829016 | MS.gene060308:CDS | 30.0% | |
| GAATTCCATGTATTATAGCA+AGG | - | chr1.2:79828364-79828383 | None:intergenic | 30.0% | |
| GCAAGATATGTTAATAGCTA+TGG | + | chr1.2:79829085-79829104 | MS.gene060308:CDS | 30.0% | |
| GCCAATAATACATATTAGTG+AGG | + | chr1.2:79828771-79828790 | MS.gene060308:intron | 30.0% | |
| TAATTATGCACCTAGTCATT+GGG | + | chr1.2:79829237-79829256 | MS.gene060308:CDS | 30.0% | |
| TTGATAGAAGTTGTTGATGT+GGG | + | chr1.2:79828441-79828460 | MS.gene060308:intron | 30.0% | |
| TTTGATAGAAGTTGTTGATG+TGG | + | chr1.2:79828440-79828459 | MS.gene060308:intron | 30.0% | |
| ! | AATATGTATTATTGGCGGAT+TGG | - | chr1.2:79828767-79828786 | None:intergenic | 30.0% |
| ! | ATTTTTCATCTAATACCAGG+TGG | + | chr1.2:79828875-79828894 | MS.gene060308:intron | 30.0% |
| !! | TTGAATAAGTTGATGAGGAA+TGG | + | chr1.2:79829358-79829377 | MS.gene060308:CDS | 30.0% |
| ACAGAGTATAAGGCAAAGTA+TGG | + | chr1.2:79829436-79829455 | MS.gene060308:CDS | 35.0% | |
| ATTGTGCTCCTATTTGATCA+CGG | - | chr1.2:79828910-79828929 | None:intergenic | 35.0% | |
| CTAATTATGCACCTAGTCAT+TGG | + | chr1.2:79829236-79829255 | MS.gene060308:CDS | 35.0% | |
| GATTTGAGAAAGGAGATTAC+AGG | + | chr1.2:79829112-79829131 | MS.gene060308:CDS | 35.0% | |
| TTTCCATATGAATTGTGTCG+CGG | - | chr1.2:79828954-79828973 | None:intergenic | 35.0% | |
| TTTGAGAAAGGAGATTACAG+GGG | + | chr1.2:79829114-79829133 | MS.gene060308:CDS | 35.0% | |
| ! | GTACTCCTTGCTATAATACA+TGG | + | chr1.2:79828356-79828375 | MS.gene060308:CDS | 35.0% |
| ! | TAGTTTAAGAAGTGGGTTTG+TGG | + | chr1.2:79829330-79829349 | MS.gene060308:CDS | 35.0% |
| ! | TCAAAAGGTGAGTTTGTTTG+TGG | + | chr1.2:79829012-79829031 | MS.gene060308:CDS | 35.0% |
| !! | AATGGTAATGGTCAAGCTAA+TGG | + | chr1.2:79829172-79829191 | MS.gene060308:CDS | 35.0% |
| !! | CAGAGTTGAATAAGTTGATG+AGG | + | chr1.2:79829353-79829372 | MS.gene060308:CDS | 35.0% |
| !! | GCTAATGGTGGATTAATTGA+AGG | + | chr1.2:79829187-79829206 | MS.gene060308:CDS | 35.0% |
| !!! | TGATGTTACTGCTCGTTTTT+TGG | + | chr1.2:79829297-79829316 | MS.gene060308:CDS | 35.0% |
| AAATGACACATGTATGGCAG+TGG | + | chr1.2:79829149-79829168 | MS.gene060308:CDS | 40.0% | |
| AGTAATACCTGGAGTAGAGA+GGG | - | chr1.2:79828417-79828436 | None:intergenic | 40.0% | |
| ATTCCGCGACACAATTCATA+TGG | + | chr1.2:79828948-79828967 | MS.gene060308:CDS | 40.0% | |
| GAGCAGTAACATCATAACCT+TGG | - | chr1.2:79829291-79829310 | None:intergenic | 40.0% | |
| GGAAGACTGTTAATCAGTTG+TGG | + | chr1.2:79829413-79829432 | MS.gene060308:CDS | 40.0% | |
| GTACGTTTCCGTGATCAAAT+AGG | + | chr1.2:79828899-79828918 | MS.gene060308:CDS | 40.0% | |
| TACCTGTGGAAATAAACCCT+CGG | - | chr1.2:79828811-79828830 | None:intergenic | 40.0% | |
| TAGTAATACCTGGAGTAGAG+AGG | - | chr1.2:79828418-79828437 | None:intergenic | 40.0% | |
| TCAGTTGTGGACAGAGTATA+AGG | + | chr1.2:79829426-79829445 | MS.gene060308:CDS | 40.0% | |
| ! | CTATGGTGGTGATTTGAGAA+AGG | + | chr1.2:79829102-79829121 | MS.gene060308:CDS | 40.0% |
| !!! | GAACATTTTTTCTGTCGGCA+GGG | - | chr1.2:79828994-79829013 | None:intergenic | 40.0% |
| !!! | TGAACATTTTTTCTGTCGGC+AGG | - | chr1.2:79828995-79829014 | None:intergenic | 40.0% |
| AACCAGGGCAAGGAAATAAG+TGG | + | chr1.2:79829263-79829282 | MS.gene060308:CDS | 45.0% | |
| ACACATGTATGGCAGTGGAA+TGG | + | chr1.2:79829154-79829173 | MS.gene060308:CDS | 45.0% | |
| ACCAGGGCAAGGAAATAAGT+GGG | + | chr1.2:79829264-79829283 | MS.gene060308:CDS | 45.0% | |
| CAAGGAAATAAGTGGGACCA+AGG | + | chr1.2:79829271-79829290 | MS.gene060308:CDS | 45.0% | |
| CTAGTCATTGGGTGAAACCA+GGG | + | chr1.2:79829248-79829267 | MS.gene060308:CDS | 45.0% | |
| GTATGGCAGTGGAATGGTAA+TGG | + | chr1.2:79829160-79829179 | MS.gene060308:CDS | 45.0% | |
| TACCGAGGGTTTATTTCCAC+AGG | + | chr1.2:79828806-79828825 | MS.gene060308:intron | 45.0% | |
| TTGTTTGTGGACGACATGGA+TGG | + | chr1.2:79829025-79829044 | MS.gene060308:CDS | 45.0% | |
| ! | AGGGTTTTTCTGCATTACCG+AGG | + | chr1.2:79828791-79828810 | MS.gene060308:intron | 45.0% |
| ! | GAGTTTGTTTGTGGACGACA+TGG | + | chr1.2:79829021-79829040 | MS.gene060308:CDS | 45.0% |
| ! | GGGTTTTTCTGCATTACCGA+GGG | + | chr1.2:79828792-79828811 | MS.gene060308:intron | 45.0% |
| ! | GGTAATGGTCAAGCTAATGG+TGG | + | chr1.2:79829175-79829194 | MS.gene060308:CDS | 45.0% |
| !!! | ATTTTTTCTGTCGGCAGGGT+TGG | - | chr1.2:79828990-79829009 | None:intergenic | 45.0% |
| AACAATGCCCTCTCTACTCC+AGG | + | chr1.2:79828407-79828426 | MS.gene060308:CDS | 50.0% | |
| CCTAGTCATTGGGTGAAACC+AGG | + | chr1.2:79829247-79829266 | MS.gene060308:CDS | 50.0% | |
| CCTGGTTTCACCCAATGACT+AGG | - | chr1.2:79829250-79829269 | None:intergenic | 50.0% | |
| TCCCACTTATTTCCTTGCCC+TGG | - | chr1.2:79829268-79829287 | None:intergenic | 50.0% | |
| !! | TGGAGTAGAGAGGGCATTGT+TGG | - | chr1.2:79828408-79828427 | None:intergenic | 50.0% |
| CATTGGGTGAAACCAGGGCA+AGG | + | chr1.2:79829253-79829272 | MS.gene060308:CDS | 55.0% | |
| TCACGGAAACGTACTCCACC+TGG | - | chr1.2:79828893-79828912 | None:intergenic | 55.0% | |
| !! | TGTCGGCAGGGTTGGTCTGT+TGG | - | chr1.2:79828982-79829001 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 79828332 | 79829471 | 79828332 | ID=MS.gene060308 |
| chr1.2 | mRNA | 79828332 | 79829471 | 79828332 | ID=MS.gene060308.t1;Parent=MS.gene060308 |
| chr1.2 | exon | 79828332 | 79828428 | 79828332 | ID=MS.gene060308.t1.exon1;Parent=MS.gene060308.t1 |
| chr1.2 | CDS | 79828332 | 79828428 | 79828332 | ID=cds.MS.gene060308.t1;Parent=MS.gene060308.t1 |
| chr1.2 | exon | 79828894 | 79829471 | 79828894 | ID=MS.gene060308.t1.exon2;Parent=MS.gene060308.t1 |
| chr1.2 | CDS | 79828894 | 79829471 | 79828894 | ID=cds.MS.gene060308.t1;Parent=MS.gene060308.t1 |
| Gene Sequence |
| Protein sequence |
