AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060668

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060668.t1 MTR_2g078370 96.757 185 5 1 1 185 30 213 1.96e-130 369
MS.gene060668.t1 MTR_2g090900 44.079 304 113 8 5 273 28 309 6.49e-66 209
MS.gene060668.t1 MTR_2g090900 44.079 304 113 8 5 273 28 309 7.06e-66 209
MS.gene060668.t1 MTR_4g079500 45.675 289 98 12 27 274 43 313 5.86e-59 191
MS.gene060668.t1 MTR_2g078360 98.851 87 1 0 201 287 1 87 1.60e-56 177
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060668.t1 AT3G58120 44.599 287 85 7 26 254 39 309 6.03e-61 196
MS.gene060668.t1 AT2G42380 42.366 262 92 8 29 253 42 281 1.30e-48 164
MS.gene060668.t1 AT2G42380 41.392 273 90 8 29 253 42 292 3.70e-48 163
MS.gene060668.t1 AT3G58120 40.769 260 80 7 26 227 39 282 5.82e-42 146
MS.gene060668.t1 AT2G42380 38.618 246 81 8 29 226 42 265 3.04e-33 123
MS.gene060668.t1 AT2G42380 57.576 66 19 2 29 93 42 99 4.28e-15 73.2
MS.gene060668.t1 AT2G42380 57.576 66 19 2 29 93 42 99 4.28e-15 73.2

Find 87 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTGCAATCCGTTGCTTTA+TGG 0.114715 2.2:-16285753 None:intergenic
CTTCATTGTTATTGCTATTT+TGG 0.139041 2.2:-16284391 None:intergenic
TTCAGTGCAGGTGATGTTTC+CGG 0.261998 2.2:-16284549 None:intergenic
GTCACCACGAGTTGCATTCT+TGG 0.266371 2.2:+16285694 MS.gene060668:CDS
CAGAGAACATGGATATTAAT+TGG 0.292855 2.2:-16284295 None:intergenic
GGTGGCTGTGGTCGTGGTGG+TGG 0.293722 2.2:-16284060 None:intergenic
GATGGTCAGAAGTATTATTC+TGG 0.294836 2.2:-16284085 None:intergenic
TGTTGTTGTGGCTGTGATGT+TGG 0.396153 2.2:-16286740 None:intergenic
AGGAGTGGTGGTGGCGGTGG+TGG 0.401882 2.2:+16284248 MS.gene060668:CDS
GTGATGGTGGTGATTGTTGA+TGG 0.405926 2.2:-16284103 None:intergenic
AGTGGTGGTGGCGGTGGTGG+AGG 0.410009 2.2:+16284251 MS.gene060668:CDS
GGCTGTGGTCGTGGTGGTGG+AGG 0.412837 2.2:-16284057 None:intergenic
CTGGGTGGTGGCTGTGGTCG+TGG 0.413794 2.2:-16284066 None:intergenic
TGCAGGAGTGGTGGTGGCGG+TGG 0.414536 2.2:+16284245 MS.gene060668:CDS
CCAACAAAACATACAAAAGA+TGG 0.422195 2.2:+16286664 MS.gene060668:CDS
TGTGGCTGTGATGTTGGTGT+TGG 0.423054 2.2:-16286734 None:intergenic
ATGTTCTCTGACAATGTTGC+CGG 0.425101 2.2:+16284308 MS.gene060668:CDS
GGATTTGCTCCTTGTGCTGA+TGG 0.435960 2.2:-16286769 None:intergenic
GACTGCAGGAGTGGTGGTGG+CGG 0.440441 2.2:+16284242 MS.gene060668:CDS
CGATAAGGCGTGGGGCTCAC+AGG 0.446573 2.2:+16284168 MS.gene060668:CDS
CATCTTTGAAAAGCTTGTCC+TGG 0.451454 2.2:-16285781 None:intergenic
AAGGAGCAAATCCTCAGTTA+AGG 0.456072 2.2:+16286779 MS.gene060668:CDS
AAAGCAACGGATTGCAGCAT+TGG 0.460058 2.2:+16285757 MS.gene060668:CDS
TCATCGACGGCTTCATCCTT+AGG 0.467958 2.2:-16284479 None:intergenic
ATGGTCAGAAGTATTATTCT+GGG 0.476907 2.2:-16284084 None:intergenic
ATCTTTGAAAAGCTTGTCCT+GGG 0.477116 2.2:-16285780 None:intergenic
TCCTCGACTTCTCCTCGATA+AGG 0.487643 2.2:+16284153 MS.gene060668:CDS
ACGGATTGCAGCATTGGCCC+AGG 0.491712 2.2:+16285763 MS.gene060668:CDS
ATCCACAACGGTTTCAGTGC+AGG 0.497157 2.2:-16284561 None:intergenic
AGAAGTATTATTCTGGGTGG+TGG 0.505308 2.2:-16284078 None:intergenic
AGGAGCAAATCCTCAGTTAA+GGG 0.505684 2.2:+16286780 MS.gene060668:CDS
AGAAGGAAATAGAAAGGTTG+AGG 0.509958 2.2:+16286630 MS.gene060668:CDS
GCATCCAACACCATTGCCAT+TGG 0.510208 2.2:-16284419 None:intergenic
CAGTAAGAATCATACGTTGA+TGG 0.511877 2.2:-16285718 None:intergenic
ACTTCTCCTCGATAAGGCGT+GGG 0.514254 2.2:+16284159 MS.gene060668:CDS
GAAGACTGCAGGAGTGGTGG+TGG 0.518418 2.2:+16284239 MS.gene060668:CDS
ACACGAACTGTCGTCATCGA+CGG 0.522351 2.2:-16284492 None:intergenic
ATTATTCTGGGTGGTGGCTG+TGG 0.527672 2.2:-16284072 None:intergenic
TGGTGGAGGATTTGATAGAC+TGG 0.528585 2.2:+16284265 MS.gene060668:CDS
CATCACAACAATTACCATCA+TGG 0.531974 2.2:+16284120 MS.gene060668:CDS
GACTTCTCCTCGATAAGGCG+TGG 0.532570 2.2:+16284158 MS.gene060668:CDS
GAAACCAATGGCAATGGTGT+TGG 0.533241 2.2:+16284415 MS.gene060668:CDS
GGCAACATTGTCAGAGAACA+TGG 0.535418 2.2:-16284306 None:intergenic
TGATGGAAAGATTGTTGTTG+TGG 0.543696 2.2:-16286752 None:intergenic
GAGGAAGGCAATAGAGTCAC+TGG 0.543901 2.2:-16284198 None:intergenic
TGGTCGGAGGATGGTGACGA+TGG 0.544700 2.2:-16284371 None:intergenic
GGTCGGAGGATGGTGACGAT+GGG 0.544837 2.2:-16284370 None:intergenic
CATTAAAGAAGGAAATAGAA+AGG 0.550196 2.2:+16286624 MS.gene060668:CDS
GCGGCAGCACCATCATCCTC+CGG 0.550627 2.2:+16284530 MS.gene060668:CDS
GGAGGATGGTGACGATGGGT+TGG 0.554167 2.2:-16284366 None:intergenic
CATGATGGTAATTGTTGTGA+TGG 0.559426 2.2:-16284119 None:intergenic
CAACAAAACATACAAAAGAT+GGG 0.561228 2.2:+16286665 MS.gene060668:CDS
ACATCAAGAAGCATTAAAGA+AGG 0.562831 2.2:+16286613 MS.gene060668:CDS
CTCGAGGAAGACTGCAGGAG+TGG 0.574880 2.2:+16284233 MS.gene060668:CDS
TCATCATGAGGTGAAGCCTA+AGG 0.575470 2.2:+16284463 MS.gene060668:CDS
GGTGGTGGCTGTGGTCGTGG+TGG 0.585497 2.2:-16284063 None:intergenic
GACGTTGATGACAACGGCAA+AGG 0.589110 2.2:-16284344 None:intergenic
GACCCTCTTGGGATCCACAA+CGG 0.590222 2.2:-16284573 None:intergenic
TGATGAAGATAATGCTCTTG+AGG 0.591787 2.2:-16284446 None:intergenic
CAAAGGCGGGAAAGATACGC+CGG 0.592526 2.2:-16284327 None:intergenic
TTGATGACAACGGCAAAGGC+GGG 0.594747 2.2:-16284340 None:intergenic
GAGAAATGATGTCTCGAGGA+AGG 0.595132 2.2:-16284213 None:intergenic
CATTTCTCGAGGAAGACTGC+AGG 0.596326 2.2:+16284228 MS.gene060668:CDS
GTTGATGACAACGGCAAAGG+CGG 0.597344 2.2:-16284341 None:intergenic
AGGAATTCGTCTATCCATGA+TGG 0.597813 2.2:-16284134 None:intergenic
GTGAGCCCCACGCCTTATCG+AGG 0.599484 2.2:-16284165 None:intergenic
TAAGGCGTGGGGCTCACAGG+CGG 0.603287 2.2:+16284171 MS.gene060668:CDS
GTGATGTTTCCGGAGGATGA+TGG 0.605743 2.2:-16284539 None:intergenic
GTTAATAGTAACATTAACAA+TGG 0.606660 2.2:+16286695 MS.gene060668:CDS
TGAAGAGAAACCAATGGCAA+TGG 0.607257 2.2:+16284409 MS.gene060668:CDS
CACCTGCACTGAAACCGTTG+TGG 0.612559 2.2:+16284559 MS.gene060668:CDS
CCTCGAGACATCATTTCTCG+AGG 0.612795 2.2:+16284217 MS.gene060668:CDS
GAGGAAGACTGCAGGAGTGG+TGG 0.618813 2.2:+16284236 MS.gene060668:CDS
AACCGTTGTGGATCCCAAGA+GGG 0.623443 2.2:+16284571 MS.gene060668:CDS
GATGGTAATTGTTGTGATGG+TGG 0.623528 2.2:-16284116 None:intergenic
TAACAATGAAGAGAAACCAA+TGG 0.626582 2.2:+16284403 MS.gene060668:CDS
AGAGCATTATCTTCATCATG+AGG 0.643559 2.2:+16284451 MS.gene060668:CDS
CCTCGAGAAATGATGTCTCG+AGG 0.646437 2.2:-16284217 None:intergenic
GCCTTATCGAGGAGAAGTCG+AGG 0.651890 2.2:-16284154 None:intergenic
AGTGCAGGTGATGTTTCCGG+AGG 0.655701 2.2:-16284546 None:intergenic
CTTCTCCTCGATAAGGCGTG+GGG 0.658846 2.2:+16284160 MS.gene060668:CDS
ACAATCTTTCCATCAGCACA+AGG 0.663801 2.2:+16286760 MS.gene060668:CDS
AAACCGTTGTGGATCCCAAG+AGG 0.664635 2.2:+16284570 MS.gene060668:CDS
GTCAGAAGTATTATTCTGGG+TGG 0.668741 2.2:-16284081 None:intergenic
ACAACAGTGCCATAAAGCAA+CGG 0.673543 2.2:+16285744 MS.gene060668:CDS
GGGTTGGACGTTGATGACAA+CGG 0.716785 2.2:-16284350 None:intergenic
TGGTCCAAGAATGCAACTCG+TGG 0.753629 2.2:-16285698 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAATATTTTAGAAATAAAAT+GGG - chr2.2:16286569-16286588 None:intergenic 10.0%
!!! TAATTATCAAAACAATTTTA+TGG - chr2.2:16285017-16285036 None:intergenic 10.0%
!!! TCAATATTTTAGAAATAAAA+TGG - chr2.2:16286570-16286589 None:intergenic 10.0%
!! TGAAAAATGTAAAAATGAAT+TGG + chr2.2:16285612-16285631 MS.gene060668:intron 15.0%
!! TTTCATATTGTATGTAAAAT+TGG + chr2.2:16285568-16285587 MS.gene060668:intron 15.0%
!!! GTAATTTTTGTTAATTAGAA+AGG + chr2.2:16285891-16285910 MS.gene060668:intron 15.0%
!!! TAGTAAATATTTGACTTTTA+TGG + chr2.2:16285260-16285279 MS.gene060668:intron 15.0%
!! AAAAAATTCTCTGTTAATTG+AGG + chr2.2:16284813-16284832 MS.gene060668:intron 20.0%
!! AAAAATTCTCTGTTAATTGA+GGG + chr2.2:16284814-16284833 MS.gene060668:intron 20.0%
!! AAAATTAATGATCAAAACCA+TGG + chr2.2:16285047-16285066 MS.gene060668:intron 20.0%
!! AATCTGACTATAATACAATT+GGG - chr2.2:16286390-16286409 None:intergenic 20.0%
!! ATTAATCAAGCTTATAAAGA+TGG - chr2.2:16285838-16285857 None:intergenic 20.0%
!! CTATGATATGATATAGTAAA+TGG + chr2.2:16284638-16284657 MS.gene060668:intron 20.0%
!! CTTAAACAAATTAATATCCA+TGG - chr2.2:16285067-16285086 None:intergenic 20.0%
!! GTTAATAGTAACATTAACAA+TGG + chr2.2:16286695-16286714 MS.gene060668:CDS 20.0%
!! TGATAATAAGCAATTAGATT+TGG - chr2.2:16285409-16285428 None:intergenic 20.0%
!! TTAGTTCAATTATAGTAAAC+CGG + chr2.2:16286498-16286517 MS.gene060668:intron 20.0%
!!! AATAAATTGAAAGTTTTTGC+TGG + chr2.2:16284913-16284932 MS.gene060668:intron 20.0%
!!! TAGATTTGGAATTTTTCATA+TGG + chr2.2:16286046-16286065 MS.gene060668:intron 20.0%
!!! TTCAAAAATGTGTTTTTGTA+GGG - chr2.2:16286079-16286098 None:intergenic 20.0%
!!! TTGTATATATACCAGAATTT+TGG + chr2.2:16285430-16285449 MS.gene060668:intron 20.0%
!!! TTTCAAAAATGTGTTTTTGT+AGG - chr2.2:16286080-16286099 None:intergenic 20.0%
!!! TTTTCTTCAAGAAAAAACAA+TGG - chr2.2:16284796-16284815 None:intergenic 20.0%
! AATTGTAGTATAGATATGCA+TGG + chr2.2:16284942-16284961 MS.gene060668:intron 25.0%
! AGTTTATCCTAGTTTAATCT+TGG - chr2.2:16286427-16286446 None:intergenic 25.0%
! CAACAAAACATACAAAAGAT+GGG + chr2.2:16286665-16286684 MS.gene060668:CDS 25.0%
! CAATCTGACTATAATACAAT+TGG - chr2.2:16286391-16286410 None:intergenic 25.0%
! CATTAAAGAAGGAAATAGAA+AGG + chr2.2:16286624-16286643 MS.gene060668:CDS 25.0%
! GAAAAATGTGAAAATGAAGA+CGG + chr2.2:16285658-16285677 MS.gene060668:intron 25.0%
! GCTGTTAAAAAAATCACTTT+AGG + chr2.2:16286258-16286277 MS.gene060668:intron 25.0%
! GTTTAAACCAAGATTAAACT+AGG + chr2.2:16286417-16286436 MS.gene060668:intron 25.0%
! TATGATCATATTGAGATAGT+GGG + chr2.2:16286288-16286307 MS.gene060668:intron 25.0%
! TATTATAGTCAGATTGAACT+TGG + chr2.2:16286395-16286414 MS.gene060668:intron 25.0%
! TTATGATCATATTGAGATAG+TGG + chr2.2:16286287-16286306 MS.gene060668:intron 25.0%
! TTTCTTAATTGTGTAAGACT+GGG - chr2.2:16284769-16284788 None:intergenic 25.0%
!! TAAACACATTTAAAGTCACA+GGG + chr2.2:16285283-16285302 MS.gene060668:intron 25.0%
!! TTCAATTGAACATATTGGTT+TGG - chr2.2:16286340-16286359 None:intergenic 25.0%
!! TTTTCTTAATTGTGTAAGAC+TGG - chr2.2:16284770-16284789 None:intergenic 25.0%
!!! ATTGTTATTGCTATTTTGGT+CGG - chr2.2:16284390-16284409 None:intergenic 25.0%
!!! CTTCATTGTTATTGCTATTT+TGG - chr2.2:16284394-16284413 None:intergenic 25.0%
!!! TGTTGATAATCACTTAGATT+TGG + chr2.2:16286032-16286051 MS.gene060668:intron 25.0%
AATACATTTCTGAGCTTGAA+AGG + chr2.2:16285495-16285514 MS.gene060668:intron 30.0%
ATCATAAATAGAACGTGCAT+GGG - chr2.2:16284682-16284701 None:intergenic 30.0%
CAACTACAAAACGATACTTA+GGG - chr2.2:16286196-16286215 None:intergenic 30.0%
CAGAGAACATGGATATTAAT+TGG - chr2.2:16284298-16284317 None:intergenic 30.0%
CATGGTTTAATTGAATCAAC+CGG - chr2.2:16286520-16286539 None:intergenic 30.0%
CCAACAAAACATACAAAAGA+TGG + chr2.2:16286664-16286683 MS.gene060668:CDS 30.0%
GAAGATCAACAAACAATTCT+AGG - chr2.2:16285998-16286017 None:intergenic 30.0%
GTTCGTTCAATTGAACATAT+TGG - chr2.2:16286345-16286364 None:intergenic 30.0%
TAACAATGAAGAGAAACCAA+TGG + chr2.2:16284403-16284422 MS.gene060668:CDS 30.0%
TATCATAAATAGAACGTGCA+TGG - chr2.2:16284683-16284702 None:intergenic 30.0%
! AAACACATTTAAAGTCACAG+GGG + chr2.2:16285284-16285303 MS.gene060668:intron 30.0%
! CCATCTTTTGTATGTTTTGT+TGG - chr2.2:16286667-16286686 None:intergenic 30.0%
! GTAAACACATTTAAAGTCAC+AGG + chr2.2:16285282-16285301 MS.gene060668:intron 30.0%
! TATCGTTTTGTAGTTGGATT+GGG + chr2.2:16286200-16286219 MS.gene060668:intron 30.0%
!! ACATCAAGAAGCATTAAAGA+AGG + chr2.2:16286613-16286632 MS.gene060668:CDS 30.0%
!! ATGGTCAGAAGTATTATTCT+GGG - chr2.2:16284087-16284106 None:intergenic 30.0%
!!! ATACCAGAATTTTGGCTAAT+AGG + chr2.2:16285438-16285457 MS.gene060668:intron 30.0%
AAATGTGAAAATGAAGACGG+AGG + chr2.2:16285661-16285680 MS.gene060668:intron 35.0%
AACCAACTTTGATTCAACCA+TGG - chr2.2:16286457-16286476 None:intergenic 35.0%
AACCATGGTTGAATCAAAGT+TGG + chr2.2:16286452-16286471 MS.gene060668:intron 35.0%
AACTAATTCTTCATGCTAGG+TGG + chr2.2:16286153-16286172 MS.gene060668:intron 35.0%
AGAAGGAAATAGAAAGGTTG+AGG + chr2.2:16286630-16286649 MS.gene060668:CDS 35.0%
AGGAACTAATTCTTCATGCT+AGG + chr2.2:16286150-16286169 MS.gene060668:intron 35.0%
AGGATAAACTAGTTGAACCA+TGG + chr2.2:16286437-16286456 MS.gene060668:intron 35.0%
ATTGGGAATGTCGAATTGAA+CGG - chr2.2:16286373-16286392 None:intergenic 35.0%
CAGTAAGAATCATACGTTGA+TGG - chr2.2:16285721-16285740 None:intergenic 35.0%
CATCACAACAATTACCATCA+TGG + chr2.2:16284120-16284139 MS.gene060668:CDS 35.0%
CATGATGGTAATTGTTGTGA+TGG - chr2.2:16284122-16284141 None:intergenic 35.0%
CCAACTACAAAACGATACTT+AGG - chr2.2:16286197-16286216 None:intergenic 35.0%
CTAAAGTGACTAATTCTCAC+AGG - chr2.2:16286132-16286151 None:intergenic 35.0%
TGATGAAGATAATGCTCTTG+AGG - chr2.2:16284449-16284468 None:intergenic 35.0%
TTCGTGTAAAAATGAGGTAG+CGG + chr2.2:16284508-16284527 MS.gene060668:CDS 35.0%
TTGCCTATTAGCCAAAATTC+TGG - chr2.2:16285444-16285463 None:intergenic 35.0%
! AACACATTTAAAGTCACAGG+GGG + chr2.2:16285285-16285304 MS.gene060668:intron 35.0%
! AAGGAGTGTAACATCTTTAC+AGG + chr2.2:16285514-16285533 MS.gene060668:intron 35.0%
! AGAGCATTATCTTCATCATG+AGG + chr2.2:16284451-16284470 MS.gene060668:CDS 35.0%
! ATCGTTTTGTAGTTGGATTG+GGG + chr2.2:16286201-16286220 MS.gene060668:intron 35.0%
! ATCTTTGAAAAGCTTGTCCT+GGG - chr2.2:16285783-16285802 None:intergenic 35.0%
! CTGTGAGAATTAGTCACTTT+AGG + chr2.2:16286130-16286149 MS.gene060668:intron 35.0%
! GTATCGTTTTGTAGTTGGAT+TGG + chr2.2:16286199-16286218 MS.gene060668:intron 35.0%
! TGATGGAAAGATTGTTGTTG+TGG - chr2.2:16286755-16286774 None:intergenic 35.0%
!! CCTAAGTATCGTTTTGTAGT+TGG + chr2.2:16286194-16286213 MS.gene060668:intron 35.0%
!! GATGGTCAGAAGTATTATTC+TGG - chr2.2:16284088-16284107 None:intergenic 35.0%
ACAACAGTGCCATAAAGCAA+CGG + chr2.2:16285744-16285763 MS.gene060668:CDS 40.0%
ACAATCTTTCCATCAGCACA+AGG + chr2.2:16286760-16286779 MS.gene060668:CDS 40.0%
AGGAATTCGTCTATCCATGA+TGG - chr2.2:16284137-16284156 None:intergenic 40.0%
ATAAATAGAACGTGCATGGG+TGG - chr2.2:16284679-16284698 None:intergenic 40.0%
ATCGAACTAGTGAGACATCT+GGG - chr2.2:16286544-16286563 None:intergenic 40.0%
ATGTTCTCTGACAATGTTGC+CGG + chr2.2:16284308-16284327 MS.gene060668:CDS 40.0%
CCACAAAATTCTTGCATGGA+TGG + chr2.2:16284743-16284762 MS.gene060668:intron 40.0%
CGACAGTTCGTGTAAAAATG+AGG + chr2.2:16284502-16284521 MS.gene060668:CDS 40.0%
GATGGTAATTGTTGTGATGG+TGG - chr2.2:16284119-16284138 None:intergenic 40.0%
GTTGCCACAAAATTCTTGCA+TGG + chr2.2:16284739-16284758 MS.gene060668:intron 40.0%
TGAAGAGAAACCAATGGCAA+TGG + chr2.2:16284409-16284428 MS.gene060668:CDS 40.0%
! AGATTACCTTTTGACCCTCT+TGG - chr2.2:16284588-16284607 None:intergenic 40.0%
! CAGGACAAGCTTTTCAAAGA+TGG + chr2.2:16285782-16285801 MS.gene060668:CDS 40.0%
! CATCTTTGAAAAGCTTGTCC+TGG - chr2.2:16285784-16285803 None:intergenic 40.0%
! CCATCCATGCAAGAATTTTG+TGG - chr2.2:16284746-16284765 None:intergenic 40.0%
! GATTACCTTTTGACCCTCTT+GGG - chr2.2:16284587-16284606 None:intergenic 40.0%
!! AGAAGTATTATTCTGGGTGG+TGG - chr2.2:16284081-16284100 None:intergenic 40.0%
!! GTCAGAAGTATTATTCTGGG+TGG - chr2.2:16284084-16284103 None:intergenic 40.0%
!!! GTTATTGCTATTTTGGTCGG+AGG - chr2.2:16284387-16284406 None:intergenic 40.0%
CTAATAGGCAATCAGCTCAG+AGG + chr2.2:16285453-16285472 MS.gene060668:intron 45.0%
GAAACCAATGGCAATGGTGT+TGG + chr2.2:16284415-16284434 MS.gene060668:CDS 45.0%
GAGAAATGATGTCTCGAGGA+AGG - chr2.2:16284216-16284235 None:intergenic 45.0%
GATCGAACTAGTGAGACATC+TGG - chr2.2:16286545-16286564 None:intergenic 45.0%
GGCAACATTGTCAGAGAACA+TGG - chr2.2:16284309-16284328 None:intergenic 45.0%
TCATCATGAGGTGAAGCCTA+AGG + chr2.2:16284463-16284482 MS.gene060668:CDS 45.0%
TGGTGGAGGATTTGATAGAC+TGG + chr2.2:16284265-16284284 MS.gene060668:CDS 45.0%
TGTTGTTGTGGCTGTGATGT+TGG - chr2.2:16286743-16286762 None:intergenic 45.0%
TTCAGTGCAGGTGATGTTTC+CGG - chr2.2:16284552-16284571 None:intergenic 45.0%
! CTCTGTTAATTGAGGGTACG+TGG + chr2.2:16284821-16284840 MS.gene060668:intron 45.0%
! GTGTAAAAATGAGGTAGCGG+CGG + chr2.2:16284511-16284530 MS.gene060668:CDS 45.0%
! TGCTGCAATCCGTTGCTTTA+TGG - chr2.2:16285756-16285775 None:intergenic 45.0%
!! AAAGCAACGGATTGCAGCAT+TGG + chr2.2:16285757-16285776 MS.gene060668:CDS 45.0%
!! GTGATGGTGGTGATTGTTGA+TGG - chr2.2:16284106-16284125 None:intergenic 45.0%
!!! TTGCTATTTTGGTCGGAGGA+TGG - chr2.2:16284383-16284402 None:intergenic 45.0%
!! AGAAATATATATAATAAATT+AGG - chr2.2:16285815-16285834 None:intergenic 5.0%
!! ATATTTCATATAATAAAATA+AGG + chr2.2:16284700-16284719 MS.gene060668:intron 5.0%
AAACCGTTGTGGATCCCAAG+AGG + chr2.2:16284570-16284589 MS.gene060668:CDS 50.0%
AACCGTTGTGGATCCCAAGA+GGG + chr2.2:16284571-16284590 MS.gene060668:CDS 50.0%
AATAGAACGTGCATGGGTGG+TGG - chr2.2:16284676-16284695 None:intergenic 50.0%
ACACGAACTGTCGTCATCGA+CGG - chr2.2:16284495-16284514 None:intergenic 50.0%
ACTTCTCCTCGATAAGGCGT+GGG + chr2.2:16284159-16284178 MS.gene060668:CDS 50.0%
ATCCACAACGGTTTCAGTGC+AGG - chr2.2:16284564-16284583 None:intergenic 50.0%
CATTTCTCGAGGAAGACTGC+AGG + chr2.2:16284228-16284247 MS.gene060668:CDS 50.0%
CCTCGAGAAATGATGTCTCG+AGG - chr2.2:16284220-16284239 None:intergenic 50.0%
CCTCGAGACATCATTTCTCG+AGG + chr2.2:16284217-16284236 MS.gene060668:CDS 50.0%
CTAGTGAGACATCTGGGTCA+TGG - chr2.2:16286538-16286557 None:intergenic 50.0%
GAGGAAGGCAATAGAGTCAC+TGG - chr2.2:16284201-16284220 None:intergenic 50.0%
GCAATCAGCTCAGAGGTCAA+GGG + chr2.2:16285460-16285479 MS.gene060668:intron 50.0%
GCATCCAACACCATTGCCAT+TGG - chr2.2:16284422-16284441 None:intergenic 50.0%
GTCACCACGAGTTGCATTCT+TGG + chr2.2:16285694-16285713 MS.gene060668:CDS 50.0%
GTGATGTTTCCGGAGGATGA+TGG - chr2.2:16284542-16284561 None:intergenic 50.0%
TCATCGACGGCTTCATCCTT+AGG - chr2.2:16284482-16284501 None:intergenic 50.0%
TCCTCGACTTCTCCTCGATA+AGG + chr2.2:16284153-16284172 MS.gene060668:CDS 50.0%
TGGATCCCAAGAGGGTCAAA+AGG + chr2.2:16284579-16284598 MS.gene060668:intron 50.0%
TGGTCCAAGAATGCAACTCG+TGG - chr2.2:16285701-16285720 None:intergenic 50.0%
TGTGGCTGTGATGTTGGTGT+TGG - chr2.2:16286737-16286756 None:intergenic 50.0%
! ATTATTCTGGGTGGTGGCTG+TGG - chr2.2:16284075-16284094 None:intergenic 50.0%
! GGATTTGCTCCTTGTGCTGA+TGG - chr2.2:16286772-16286791 None:intergenic 50.0%
! TTGATGACAACGGCAAAGGC+GGG - chr2.2:16284343-16284362 None:intergenic 50.0%
!! GACGTTGATGACAACGGCAA+AGG - chr2.2:16284347-16284366 None:intergenic 50.0%
!! GGGTTGGACGTTGATGACAA+CGG - chr2.2:16284353-16284372 None:intergenic 50.0%
!! GTTGATGACAACGGCAAAGG+CGG - chr2.2:16284344-16284363 None:intergenic 50.0%
AGTGCAGGTGATGTTTCCGG+AGG - chr2.2:16284549-16284568 None:intergenic 55.0%
CAAAGGCGGGAAAGATACGC+CGG - chr2.2:16284330-16284349 None:intergenic 55.0%
CACCTGCACTGAAACCGTTG+TGG + chr2.2:16284559-16284578 MS.gene060668:CDS 55.0%
CTTCTCCTCGATAAGGCGTG+GGG + chr2.2:16284160-16284179 MS.gene060668:CDS 55.0%
GACCCTCTTGGGATCCACAA+CGG - chr2.2:16284576-16284595 None:intergenic 55.0%
GACTTCTCCTCGATAAGGCG+TGG + chr2.2:16284158-16284177 MS.gene060668:CDS 55.0%
GCCTTATCGAGGAGAAGTCG+AGG - chr2.2:16284157-16284176 None:intergenic 55.0%
GGCAATCAGCTCAGAGGTCA+AGG + chr2.2:16285459-16285478 MS.gene060668:intron 55.0%
CTCGAGGAAGACTGCAGGAG+TGG + chr2.2:16284233-16284252 MS.gene060668:CDS 60.0%
GAAGACTGCAGGAGTGGTGG+TGG + chr2.2:16284239-16284258 MS.gene060668:CDS 60.0%
GAGGAAGACTGCAGGAGTGG+TGG + chr2.2:16284236-16284255 MS.gene060668:CDS 60.0%
! CGTGCATGGGTGGTGGTGAT+TGG - chr2.2:16284669-16284688 None:intergenic 60.0%
! GGAGGATGGTGACGATGGGT+TGG - chr2.2:16284369-16284388 None:intergenic 60.0%
! GGTCGGAGGATGGTGACGAT+GGG - chr2.2:16284373-16284392 None:intergenic 60.0%
! TGGTCGGAGGATGGTGACGA+TGG - chr2.2:16284374-16284393 None:intergenic 60.0%
!! ACGGATTGCAGCATTGGCCC+AGG + chr2.2:16285763-16285782 MS.gene060668:CDS 60.0%
CGATAAGGCGTGGGGCTCAC+AGG + chr2.2:16284168-16284187 MS.gene060668:CDS 65.0%
GCGGCAGCACCATCATCCTC+CGG + chr2.2:16284530-16284549 MS.gene060668:CDS 65.0%
GTGAGCCCCACGCCTTATCG+AGG - chr2.2:16284168-16284187 None:intergenic 65.0%
TAAGGCGTGGGGCTCACAGG+CGG + chr2.2:16284171-16284190 MS.gene060668:CDS 65.0%
!! GACTGCAGGAGTGGTGGTGG+CGG + chr2.2:16284242-16284261 MS.gene060668:CDS 65.0%
GGTGGCTGTGGTCGTGGTGG+TGG - chr2.2:16284063-16284082 None:intergenic 70.0%
! CTGGGTGGTGGCTGTGGTCG+TGG - chr2.2:16284069-16284088 None:intergenic 70.0%
! GGTGGTGGCTGTGGTCGTGG+TGG - chr2.2:16284066-16284085 None:intergenic 70.0%
!! AGGAGTGGTGGTGGCGGTGG+TGG + chr2.2:16284248-16284267 MS.gene060668:CDS 70.0%
!! AGTGGTGGTGGCGGTGGTGG+AGG + chr2.2:16284251-16284270 MS.gene060668:CDS 70.0%
!! GGCTGTGGTCGTGGTGGTGG+AGG - chr2.2:16284060-16284079 None:intergenic 70.0%
!! TGCAGGAGTGGTGGTGGCGG+TGG + chr2.2:16284245-16284264 MS.gene060668:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 16284044 16286799 16284044 ID=MS.gene060668
chr2.2 mRNA 16284044 16286799 16284044 ID=MS.gene060668.t1;Parent=MS.gene060668
chr2.2 exon 16284044 16284592 16284044 ID=MS.gene060668.t1.exon1;Parent=MS.gene060668.t1
chr2.2 CDS 16284044 16284592 16284044 ID=cds.MS.gene060668.t1;Parent=MS.gene060668.t1
chr2.2 exon 16285677 16285803 16285677 ID=MS.gene060668.t1.exon2;Parent=MS.gene060668.t1
chr2.2 CDS 16285677 16285803 16285677 ID=cds.MS.gene060668.t1;Parent=MS.gene060668.t1
chr2.2 exon 16286612 16286799 16286612 ID=MS.gene060668.t1.exon3;Parent=MS.gene060668.t1
chr2.2 CDS 16286612 16286799 16286612 ID=cds.MS.gene060668.t1;Parent=MS.gene060668.t1
Gene Sequence

>MS.gene060668

ATGTCAAACATCACCTCCACCACCACGACCACAGCCACCACCCAGAATAATACTTCTGACCATCAACAATCACCACCATCACAACAATTACCATCATGGATAGACGAATTCCTCGACTTCTCCTCGATAAGGCGTGGGGCTCACAGGCGGTCTGCCAGTGACTCTATTGCCTTCCTCGAGACATCATTTCTCGAGGAAGACTGCAGGAGTGGTGGTGGCGGTGGTGGAGGATTTGATAGACTGGATGATGACCAATTAATATCCATGTTCTCTGACAATGTTGCCGGCGTATCTTTCCCGCCTTTGCCGTTGTCATCAACGTCCAACCCATCGTCACCATCCTCCGACCAAAATAGCAATAACAATGAAGAGAAACCAATGGCAATGGTGTTGGATGCACAGCCTCAAGAGCATTATCTTCATCATGAGGTGAAGCCTAAGGATGAAGCCGTCGATGACGACAGTTCGTGTAAAAATGAGGTAGCGGCGGCAGCACCATCATCCTCCGGAAACATCACCTGCACTGAAACCGTTGTGGATCCCAAGAGGGTCAAAAGGTAATCTTAGTATAATCTTATACAATATTATTTTTATCTATGATATGATATAGTAAATGGTTTCACCAATCACCACCACCCATGCACGTTCTATTTATGATATTTCATATAATAAAATAAGGTCGTTCATAGAATTTAGTTGCCACAAAATTCTTGCATGGATGGCCCAGTCTTACACAATTAAGAAAATGTCCATTGTTTTTTCTTGAAGAAAAAAATTCTCTGTTAATTGAGGGTACGTGGCTTGCTGAATGTTACATTTTTCAATGATAAATTAATAAATTATTATAAAACTGAGAAATTCATGAGTAGAATAAATTGAAAGTTTTTGCTGGATATAAAATTGTAGTATAGATATGCATGGTATATATAAGCAAAAGCAATTTATACACTAAATTGTTATAGTAAAAAAACCATAAAATTGTTTTGATAATTATTTAATTTATAAAATTAATGATCAAAACCATGGATATTAATTTGTTTAAGATTCATCAAATGTAAATAATTTTAACATTAGAGATACTTATCTTTGAAAACTTAAACAATTGAATGAAAACACTTGTAAATAATTTTTAGTACGTATTAAGTTTTCTCAACAAGATTTGAATGAAACTATAAAGAGTTTTTACACTTGTCAATCAATCACAACTATAATAACATAGTAAATATTTGACTTTTATGGTAAACACATTTAAAGTCACAGGGGGTACACACATGATTGTCAATTTGTCATTGAAGAACAATTAAACTAAAATATTATACTATATTATATAATATATACGTATAAAATGAAATTATGTAGATGCCAAATCTAATTGCTTATTATCATTTGTATATATACCAGAATTTTGGCTAATAGGCAATCAGCTCAGAGGTCAAGGGTGAGAAAGTTACAATACATTTCTGAGCTTGAAAGGAGTGTAACATCTTTACAGGTGAATAAATAGTTTAACTATTTGTTTTTTCTTTTCATATTGTATGTAAAATTGGATTTATGCAAATTAGTAATTGTGAAAAATGTAAAAATGAATTGGATTTATGCAAATGAATTAATTGTGAAAAATGTGAAAATGAAGACGGAGGTTTCAGCACTGTCACCACGAGTTGCATTCTTGGACCATCAACGTATGATTCTTACTGTCGACAACAGTGCCATAAAGCAACGGATTGCAGCATTGGCCCAGGACAAGCTTTTCAAAGATGGTACAATACCTAATTTATTATATATATTTCTCCATCTTTATAAGCTTGATTAATTTTCAAACTTTATACACAAAATATCACTAATTAGTAATTTTTGTTAATTAGAAAGGTAAATGTTAAAGACTAATATGATTTTTTTAGTATAATTTTCACAGTAAATTCAAATTTTTAGTGAAATAATGAAGTCATATCCTAGAATTGTTTGTTGATCTTCATGAATAATTTTGATGTTGATAATCACTTAGATTTGGAATTTTTCATATGGAGAATGTCCCTACAAAAACACATTTTTGAAATAAATGAGTTTGAAATTCTTTTATTCGATCCTGTGAGAATTAGTCACTTTAGGAACTAATTCTTCATGCTAGGTGGAGTTTTGTTTGTGTTTTCCCTAAGTATCGTTTTGTAGTTGGATTGGGGTTGTAATTTTTTTTAATATATAAATATATATTTTGCTGTTAAAAAAATCACTTTAGGAACTTTTTATGATCATATTGAGATAGTGGGTGTACATGTATATTTTAAAAACTGAACCAAACCAATATGTTCAATTGAACGAACGAAAACCGTTCAATTCGACATTCCCAATTGTATTATAGTCAGATTGAACTTGGTTTAAACCAAGATTAAACTAGGATAAACTAGTTGAACCATGGTTGAATCAAAGTTGGTTTAAGTAGTTTTTATTTTTTTTTTAGTTCAATTATAGTAAACCGGTTGATTCAATTAAACCATGACCCAGATGTCTCACTAGTTCGATCTCCCATTTTATTTCTAAAATATTGATATATACTTTTGAATTTATCAGCACATCAAGAAGCATTAAAGAAGGAAATAGAAAGGTTGAGGCAAATCTATCACCAACAAAACATACAAAAGATGGGAAATTCAGTTAATAGTAACATTAACAATGGTCATTCTCTTCAATCTCCAACACCAACATCACAGCCACAACAACAATCTTTCCATCAGCACAAGGAGCAAATCCTCAGTTAA
Protein sequence

>MS.gene060668.t1

MSNITSTTTTTATTQNNTSDHQQSPPSQQLPSWIDEFLDFSSIRRGAHRRSASDSIAFLETSFLEEDCRSGGGGGGGFDRLDDDQLISMFSDNVAGVSFPPLPLSSTSNPSSPSSDQNSNNNEEKPMAMVLDAQPQEHYLHHEVKPKDEAVDDDSSCKNEVAAAAPSSSGNITCTETVVDPKRTEVSALSPRVAFLDHQRMILTVDNSAIKQRIAALAQDKLFKDAHQEALKKEIERLRQIYHQQNIQKMGNSVNSNINNGHSLQSPTPTSQPQQQSFHQHKEQILS