Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060726.t1 | XP_013458098.1 | 91.7 | 60 | 5 | 0 | 1 | 60 | 132 | 191 | 3.40E-24 | 120.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060726.t1 | Q93WC4 | 44.4 | 63 | 32 | 1 | 1 | 60 | 164 | 226 | 1.3e-08 | 59.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060726.t1 | A0A072UQL9 | 91.7 | 60 | 5 | 0 | 1 | 60 | 132 | 191 | 2.4e-24 | 120.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene060726.t1 | TR | AUX/IAA |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060726 | MS.gene59936 | 0.830273 | 3.22E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060726.t1 | MTR_4g115075 | 91.667 | 60 | 5 | 0 | 1 | 60 | 132 | 191 | 4.74e-36 | 119 |
| MS.gene060726.t1 | MTR_3g106850 | 39.683 | 63 | 35 | 1 | 1 | 60 | 130 | 192 | 5.96e-12 | 57.8 |
| MS.gene060726.t1 | MTR_1g069495 | 36.047 | 86 | 29 | 2 | 1 | 60 | 150 | 235 | 2.74e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060726.t1 | AT4G32280 | 44.444 | 63 | 32 | 1 | 1 | 60 | 122 | 184 | 4.09e-14 | 63.5 |
| MS.gene060726.t1 | AT4G32280 | 44.444 | 63 | 32 | 1 | 1 | 60 | 164 | 226 | 7.38e-14 | 63.2 |
Find 10 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAACATGTCCATCAAAGTT+TGG | 0.287284 | 8.3:-12083929 | None:intergenic |
| TACTCTCAACCAAACTTTGA+TGG | 0.301864 | 8.3:+12083920 | MS.gene060726:CDS |
| ATCAAGACAAAGAAGGTGAT+TGG | 0.417147 | 8.3:+12084109 | MS.gene060726:CDS |
| TGATCAACAACAGTATGAAC+TGG | 0.501567 | 8.3:+12084083 | MS.gene060726:CDS |
| GAATTACGTGAAGGTGAAGA+TGG | 0.506755 | 8.3:+12083845 | None:intergenic |
| CTGGTTTATCAAGACAAAGA+AGG | 0.557412 | 8.3:+12084102 | MS.gene060726:CDS |
| GGTTGAGAGTATGAAATGAA+TGG | 0.558722 | 8.3:-12083908 | None:intergenic |
| TACGTGAAGGTGAAGATGGA+AGG | 0.560134 | 8.3:+12083849 | None:intergenic |
| AGGTGTAGGAATAGCTCGAA+AGG | 0.621460 | 8.3:+12083869 | MS.gene060726:CDS |
| AAGGTGAAGATGGAAGGTGT+AGG | 0.649720 | 8.3:+12083855 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTAGTAGTACTAATTATTT+TGG | - | chr8.3:12083996-12084015 | None:intergenic | 15.0% |
| ! | GTATATATAGTGTATATGCA+CGG | + | chr8.3:12084024-12084043 | MS.gene060726:intron | 25.0% |
| ! | TTAGTACTACTAATTAGTTG+AGG | + | chr8.3:12084002-12084021 | MS.gene060726:intron | 25.0% |
| TCATGTAGTTGGAAATTAAC+AGG | + | chr8.3:12084054-12084073 | MS.gene060726:intron | 30.0% | |
| ! | TCTTGCTCAAGATATTTTCA+TGG | + | chr8.3:12084133-12084152 | MS.gene060726:CDS | 30.0% |
| ACGGCATTAATTCATGTAGT+TGG | + | chr8.3:12084043-12084062 | MS.gene060726:intron | 35.0% | |
| ATCAAGACAAAGAAGGTGAT+TGG | + | chr8.3:12084109-12084128 | MS.gene060726:CDS | 35.0% | |
| CAAACATGTCCATCAAAGTT+TGG | - | chr8.3:12083932-12083951 | None:intergenic | 35.0% | |
| CTGGTTTATCAAGACAAAGA+AGG | + | chr8.3:12084102-12084121 | MS.gene060726:CDS | 35.0% | |
| TACTCTCAACCAAACTTTGA+TGG | + | chr8.3:12083920-12083939 | MS.gene060726:CDS | 35.0% | |
| TGATCAACAACAGTATGAAC+TGG | + | chr8.3:12084083-12084102 | MS.gene060726:CDS | 35.0% | |
| ! | GGTTGAGAGTATGAAATGAA+TGG | - | chr8.3:12083911-12083930 | None:intergenic | 35.0% |
| ! | TGCTCAAGATATTTTCATGG+AGG | + | chr8.3:12084136-12084155 | MS.gene060726:CDS | 35.0% |
| AGGTGTAGGAATAGCTCGAA+AGG | + | chr8.3:12083869-12083888 | MS.gene060726:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 12083858 | 12084157 | 12083858 | ID=MS.gene060726 |
| chr8.3 | mRNA | 12083858 | 12084157 | 12083858 | ID=MS.gene060726.t1;Parent=MS.gene060726 |
| chr8.3 | exon | 12083858 | 12083957 | 12083858 | ID=MS.gene060726.t1.exon1;Parent=MS.gene060726.t1 |
| chr8.3 | CDS | 12083858 | 12083957 | 12083858 | ID=cds.MS.gene060726.t1;Parent=MS.gene060726.t1 |
| chr8.3 | exon | 12084076 | 12084157 | 12084076 | ID=MS.gene060726.t1.exon2;Parent=MS.gene060726.t1 |
| chr8.3 | CDS | 12084076 | 12084157 | 12084076 | ID=cds.MS.gene060726.t1;Parent=MS.gene060726.t1 |
| Gene Sequence |
| Protein sequence |
