Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060747.t1 | XP_003629615.1 | 83.3 | 216 | 11 | 4 | 6 | 196 | 102 | 317 | 3.30E-12 | 82 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060747.t1 | A0A5B7A7V9 | 69.3 | 153 | 31 | 2 | 6 | 142 | 99 | 251 | 1.8e-12 | 82.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060747 | MS.gene069519 | 0.800365 | 1.49E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060747.t1 | MTR_8g083140 | 84.259 | 216 | 9 | 4 | 6 | 196 | 102 | 317 | 4.34e-88 | 265 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060747.t1 | AT4G08580 | 62.567 | 187 | 42 | 5 | 36 | 194 | 128 | 314 | 3.54e-39 | 139 |
| MS.gene060747.t1 | AT4G08580 | 62.567 | 187 | 42 | 5 | 36 | 194 | 128 | 314 | 3.54e-39 | 139 |
| MS.gene060747.t1 | AT5G17900 | 67.626 | 139 | 32 | 4 | 66 | 191 | 173 | 311 | 1.51e-38 | 137 |
Find 60 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACATCAATGAATTGTTAT+TGG | 0.202578 | 4.4:+71396511 | None:intergenic |
| TTTGAGGATTGAGAAGGTTT+TGG | 0.272484 | 4.4:-71397083 | None:intergenic |
| GGAGAGAAGAAGACGGATTA+AGG | 0.319392 | 4.4:+71396620 | MS.gene060747:CDS |
| AAACCTGCATTTCTACTTTG+AGG | 0.374346 | 4.4:-71397099 | None:intergenic |
| ATGGTATCTCTCTCCGACTT+TGG | 0.379028 | 4.4:-71396747 | None:intergenic |
| ATCAATGAATTGTTATTGGC+AGG | 0.417006 | 4.4:+71396515 | None:intergenic |
| AATATGAAGCTTGGAAGAAA+AGG | 0.436853 | 4.4:+71396967 | MS.gene060747:CDS |
| GGAGAAAGAAGAAGAGGAGG+AGG | 0.438698 | 4.4:+71396665 | MS.gene060747:CDS |
| GGAAGCAAGGAAGAGGAGAA+TGG | 0.439115 | 4.4:+71396812 | MS.gene060747:CDS |
| AGGTTGTTGTTGAGGAGATT+CGG | 0.445717 | 4.4:+71396859 | MS.gene060747:CDS |
| AAGAAAATGAAGAGGTTGAG+AGG | 0.454962 | 4.4:+71397027 | MS.gene060747:CDS |
| GGCGAAGAGGCAGGAAGGGT+TGG | 0.460524 | 4.4:+71396566 | MS.gene060747:CDS |
| ATGAAGAGGTTGAGAGGGTG+AGG | 0.462109 | 4.4:+71397033 | MS.gene060747:CDS |
| AGAAAAGGATCAAGCGGGAT+AGG | 0.473338 | 4.4:+71396982 | MS.gene060747:CDS |
| AGCTGAAGAGGAGGCTCTTG+AGG | 0.477467 | 4.4:+71396791 | MS.gene060747:CDS |
| CAAGCGGGATAGGGAGGACC+GGG | 0.489254 | 4.4:+71396992 | MS.gene060747:CDS |
| GGAATATGACAGAGGAGGAA+AGG | 0.492468 | 4.4:+71397054 | MS.gene060747:CDS |
| TGAAGAGGAGGCGAAGAGGC+AGG | 0.494479 | 4.4:+71396557 | MS.gene060747:CDS |
| GAAGTTGCGTCAGAGAGATC+AGG | 0.497425 | 4.4:+71396644 | MS.gene060747:CDS |
| GAAAAGGATCAAGCGGGATA+GGG | 0.505570 | 4.4:+71396983 | MS.gene060747:CDS |
| TCAGGAGAAAGAAGAAGAGG+AGG | 0.506559 | 4.4:+71396662 | MS.gene060747:CDS |
| AGATCAGGAGAAAGAAGAAG+AGG | 0.515041 | 4.4:+71396659 | MS.gene060747:CDS |
| CTCTTGAGGAAGCAAGGAAG+AGG | 0.518804 | 4.4:+71396805 | MS.gene060747:CDS |
| GAGAAGAAATGAGACGAGGC+AGG | 0.523794 | 4.4:+71396839 | MS.gene060747:CDS |
| GAGGAGGCGAAGAGGCAGGA+AGG | 0.535811 | 4.4:+71396561 | MS.gene060747:CDS |
| AGAAAATGAAGAGGTTGAGA+GGG | 0.535922 | 4.4:+71397028 | MS.gene060747:CDS |
| GACGAGGCAGGTTGTTGTTG+AGG | 0.538406 | 4.4:+71396851 | MS.gene060747:CDS |
| TTTCTTTCAACATTGCCTCC+CGG | 0.544703 | 4.4:-71397010 | None:intergenic |
| GAGACGCTCACGCTCAGCAA+TGG | 0.545728 | 4.4:-71396766 | None:intergenic |
| TCAAGCGGGATAGGGAGGAC+CGG | 0.548700 | 4.4:+71396991 | MS.gene060747:CDS |
| AGGAGGCTCTTGAGGAAGCA+AGG | 0.551309 | 4.4:+71396799 | MS.gene060747:CDS |
| AGGCAGAGGAATATGAAGCT+TGG | 0.552502 | 4.4:+71396958 | MS.gene060747:CDS |
| AGCAAGGAAGAGGAGAATGG+AGG | 0.552786 | 4.4:+71396815 | MS.gene060747:CDS |
| TGATGAACTCAACGAGGCAG+AGG | 0.554539 | 4.4:+71396944 | MS.gene060747:CDS |
| AGAACAAGATGAGGATGCTA+TGG | 0.559867 | 4.4:+71396596 | MS.gene060747:CDS |
| TGTTTCTACTATTGAAGAGG+AGG | 0.560892 | 4.4:+71396545 | MS.gene060747:CDS |
| AGGAGGCGAAGAGGCAGGAA+GGG | 0.561535 | 4.4:+71396562 | MS.gene060747:CDS |
| TATGGTGTTTGTTCCAAAGT+CGG | 0.565511 | 4.4:+71396734 | MS.gene060747:CDS |
| TGAGCGTCTCGAAGCTGAAG+AGG | 0.568841 | 4.4:+71396779 | MS.gene060747:CDS |
| GGAGTACACTGGAGTAGCTA+TGG | 0.568901 | 4.4:+71396716 | MS.gene060747:CDS |
| TTCTACTTTGAGGATTGAGA+AGG | 0.571512 | 4.4:-71397089 | None:intergenic |
| GATTGTTTCTACTATTGAAG+AGG | 0.583283 | 4.4:+71396542 | MS.gene060747:CDS |
| GGTGAGGAATATGACAGAGG+AGG | 0.583430 | 4.4:+71397049 | MS.gene060747:CDS |
| GGATTTAGAAGAACAAGATG+AGG | 0.588247 | 4.4:+71396587 | MS.gene060747:CDS |
| AAGGATCAAGCGGGATAGGG+AGG | 0.598194 | 4.4:+71396986 | MS.gene060747:CDS |
| GAGGGTGAGGAATATGACAG+AGG | 0.598749 | 4.4:+71397046 | MS.gene060747:CDS |
| TGTTGTTGAGGAGATTCGGA+AGG | 0.605323 | 4.4:+71396863 | MS.gene060747:CDS |
| GCGGGATAGGGAGGACCGGG+AGG | 0.608508 | 4.4:+71396995 | MS.gene060747:CDS |
| ACAAGATGAGGATGCTATGG+CGG | 0.622020 | 4.4:+71396599 | MS.gene060747:CDS |
| TTGGAAGAAAAGGATCAAGC+GGG | 0.622672 | 4.4:+71396977 | MS.gene060747:CDS |
| CTTGGAAGAAAAGGATCAAG+CGG | 0.624009 | 4.4:+71396976 | MS.gene060747:CDS |
| AATGTTGAAAGAAAATGAAG+AGG | 0.624932 | 4.4:+71397019 | MS.gene060747:CDS |
| GATTCTGATGAGGAGTACAC+TGG | 0.625099 | 4.4:+71396705 | MS.gene060747:CDS |
| CTATTGAAGAGGAGGCGAAG+AGG | 0.635858 | 4.4:+71396553 | MS.gene060747:CDS |
| GGAGGCGAATATTGCTGATG+TGG | 0.645251 | 4.4:+71396914 | MS.gene060747:CDS |
| GCGTCTCGAAGCTGAAGAGG+AGG | 0.648995 | 4.4:+71396782 | MS.gene060747:CDS |
| CTATGGCGGAGAGAAGAAGA+CGG | 0.676104 | 4.4:+71396613 | MS.gene060747:CDS |
| GTATGATACTGATTCTGATG+AGG | 0.693722 | 4.4:+71396695 | MS.gene060747:CDS |
| CACTGATGATGAACTCAACG+AGG | 0.706626 | 4.4:+71396938 | MS.gene060747:CDS |
| AGGAGAGAAGAAATGAGACG+AGG | 0.721838 | 4.4:+71396835 | MS.gene060747:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTCAGAAAAATATTGAAT+TGG | + | chr4.4:71396893-71396912 | MS.gene060747:CDS | 15.0% |
| ! | AATGTTGAAAGAAAATGAAG+AGG | + | chr4.4:71397019-71397038 | MS.gene060747:CDS | 25.0% |
| ! | TCAGAAAAATATTGAATTGG+AGG | + | chr4.4:71396896-71396915 | MS.gene060747:CDS | 25.0% |
| AATATGAAGCTTGGAAGAAA+AGG | + | chr4.4:71396967-71396986 | MS.gene060747:CDS | 30.0% | |
| !! | GATTGTTTCTACTATTGAAG+AGG | + | chr4.4:71396542-71396561 | MS.gene060747:CDS | 30.0% |
| AAGAAAATGAAGAGGTTGAG+AGG | + | chr4.4:71397027-71397046 | MS.gene060747:CDS | 35.0% | |
| AGAAAATGAAGAGGTTGAGA+GGG | + | chr4.4:71397028-71397047 | MS.gene060747:CDS | 35.0% | |
| GGATTTAGAAGAACAAGATG+AGG | + | chr4.4:71396587-71396606 | MS.gene060747:CDS | 35.0% | |
| TATGGTGTTTGTTCCAAAGT+CGG | + | chr4.4:71396734-71396753 | MS.gene060747:CDS | 35.0% | |
| ! | TGTTTCTACTATTGAAGAGG+AGG | + | chr4.4:71396545-71396564 | MS.gene060747:CDS | 35.0% |
| ! | TTTGAGGATTGAGAAGGTTT+TGG | - | chr4.4:71397086-71397105 | None:intergenic | 35.0% |
| !! | GTATGATACTGATTCTGATG+AGG | + | chr4.4:71396695-71396714 | MS.gene060747:CDS | 35.0% |
| AGAACAAGATGAGGATGCTA+TGG | + | chr4.4:71396596-71396615 | MS.gene060747:CDS | 40.0% | |
| AGATCAGGAGAAAGAAGAAG+AGG | + | chr4.4:71396659-71396678 | MS.gene060747:CDS | 40.0% | |
| CTTGGAAGAAAAGGATCAAG+CGG | + | chr4.4:71396976-71396995 | MS.gene060747:CDS | 40.0% | |
| TTGGAAGAAAAGGATCAAGC+GGG | + | chr4.4:71396977-71396996 | MS.gene060747:CDS | 40.0% | |
| TTTCTTTCAACATTGCCTCC+CGG | - | chr4.4:71397013-71397032 | None:intergenic | 40.0% | |
| ! | AGGTTGTTGTTGAGGAGATT+CGG | + | chr4.4:71396859-71396878 | MS.gene060747:CDS | 40.0% |
| ACAAGATGAGGATGCTATGG+CGG | + | chr4.4:71396599-71396618 | MS.gene060747:CDS | 45.0% | |
| AGAAAAGGATCAAGCGGGAT+AGG | + | chr4.4:71396982-71397001 | MS.gene060747:CDS | 45.0% | |
| AGGAGAGAAGAAATGAGACG+AGG | + | chr4.4:71396835-71396854 | MS.gene060747:CDS | 45.0% | |
| AGGCAGAGGAATATGAAGCT+TGG | + | chr4.4:71396958-71396977 | MS.gene060747:CDS | 45.0% | |
| ATGGTATCTCTCTCCGACTT+TGG | - | chr4.4:71396750-71396769 | None:intergenic | 45.0% | |
| GAAAAGGATCAAGCGGGATA+GGG | + | chr4.4:71396983-71397002 | MS.gene060747:CDS | 45.0% | |
| GGAATATGACAGAGGAGGAA+AGG | + | chr4.4:71397054-71397073 | MS.gene060747:CDS | 45.0% | |
| TCAGGAGAAAGAAGAAGAGG+AGG | + | chr4.4:71396662-71396681 | MS.gene060747:CDS | 45.0% | |
| TGTTGTTGAGGAGATTCGGA+AGG | + | chr4.4:71396863-71396882 | MS.gene060747:CDS | 45.0% | |
| ! | CACTGATGATGAACTCAACG+AGG | + | chr4.4:71396938-71396957 | MS.gene060747:CDS | 45.0% |
| ! | GATTCTGATGAGGAGTACAC+TGG | + | chr4.4:71396705-71396724 | MS.gene060747:CDS | 45.0% |
| !! | GGAGAGAAGAAGACGGATTA+AGG | + | chr4.4:71396620-71396639 | MS.gene060747:CDS | 45.0% |
| AGCAAGGAAGAGGAGAATGG+AGG | + | chr4.4:71396815-71396834 | MS.gene060747:CDS | 50.0% | |
| ATGAAGAGGTTGAGAGGGTG+AGG | + | chr4.4:71397033-71397052 | MS.gene060747:CDS | 50.0% | |
| CTATTGAAGAGGAGGCGAAG+AGG | + | chr4.4:71396553-71396572 | MS.gene060747:CDS | 50.0% | |
| CTCTTGAGGAAGCAAGGAAG+AGG | + | chr4.4:71396805-71396824 | MS.gene060747:CDS | 50.0% | |
| GAAGTTGCGTCAGAGAGATC+AGG | + | chr4.4:71396644-71396663 | MS.gene060747:CDS | 50.0% | |
| GAGAAGAAATGAGACGAGGC+AGG | + | chr4.4:71396839-71396858 | MS.gene060747:CDS | 50.0% | |
| GAGGGTGAGGAATATGACAG+AGG | + | chr4.4:71397046-71397065 | MS.gene060747:CDS | 50.0% | |
| GGAAGCAAGGAAGAGGAGAA+TGG | + | chr4.4:71396812-71396831 | MS.gene060747:CDS | 50.0% | |
| GGAGAAAGAAGAAGAGGAGG+AGG | + | chr4.4:71396665-71396684 | MS.gene060747:CDS | 50.0% | |
| GGAGGCGAATATTGCTGATG+TGG | + | chr4.4:71396914-71396933 | MS.gene060747:CDS | 50.0% | |
| GGAGTACACTGGAGTAGCTA+TGG | + | chr4.4:71396716-71396735 | MS.gene060747:CDS | 50.0% | |
| GGTGAGGAATATGACAGAGG+AGG | + | chr4.4:71397049-71397068 | MS.gene060747:CDS | 50.0% | |
| TGATGAACTCAACGAGGCAG+AGG | + | chr4.4:71396944-71396963 | MS.gene060747:CDS | 50.0% | |
| ! | CTATGGCGGAGAGAAGAAGA+CGG | + | chr4.4:71396613-71396632 | MS.gene060747:CDS | 50.0% |
| AAGGATCAAGCGGGATAGGG+AGG | + | chr4.4:71396986-71397005 | MS.gene060747:CDS | 55.0% | |
| AGCTGAAGAGGAGGCTCTTG+AGG | + | chr4.4:71396791-71396810 | MS.gene060747:CDS | 55.0% | |
| AGGAGGCTCTTGAGGAAGCA+AGG | + | chr4.4:71396799-71396818 | MS.gene060747:CDS | 55.0% | |
| TGAGCGTCTCGAAGCTGAAG+AGG | + | chr4.4:71396779-71396798 | MS.gene060747:CDS | 55.0% | |
| ! | GACGAGGCAGGTTGTTGTTG+AGG | + | chr4.4:71396851-71396870 | MS.gene060747:CDS | 55.0% |
| AGGAGGCGAAGAGGCAGGAA+GGG | + | chr4.4:71396562-71396581 | MS.gene060747:CDS | 60.0% | |
| GAGACGCTCACGCTCAGCAA+TGG | - | chr4.4:71396769-71396788 | None:intergenic | 60.0% | |
| GCGTCTCGAAGCTGAAGAGG+AGG | + | chr4.4:71396782-71396801 | MS.gene060747:CDS | 60.0% | |
| TCAAGCGGGATAGGGAGGAC+CGG | + | chr4.4:71396991-71397010 | MS.gene060747:CDS | 60.0% | |
| TGAAGAGGAGGCGAAGAGGC+AGG | + | chr4.4:71396557-71396576 | MS.gene060747:CDS | 60.0% | |
| CAAGCGGGATAGGGAGGACC+GGG | + | chr4.4:71396992-71397011 | MS.gene060747:CDS | 65.0% | |
| GAGGAGGCGAAGAGGCAGGA+AGG | + | chr4.4:71396561-71396580 | MS.gene060747:CDS | 65.0% | |
| GGCGAAGAGGCAGGAAGGGT+TGG | + | chr4.4:71396566-71396585 | MS.gene060747:CDS | 65.0% | |
| GCGGGATAGGGAGGACCGGG+AGG | + | chr4.4:71396995-71397014 | MS.gene060747:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 71396519 | 71397109 | 71396519 | ID=MS.gene060747 |
| chr4.4 | mRNA | 71396519 | 71397109 | 71396519 | ID=MS.gene060747.t1;Parent=MS.gene060747 |
| chr4.4 | exon | 71396519 | 71397109 | 71396519 | ID=MS.gene060747.t1.exon1;Parent=MS.gene060747.t1 |
| chr4.4 | CDS | 71396519 | 71397109 | 71396519 | ID=cds.MS.gene060747.t1;Parent=MS.gene060747.t1 |
| Gene Sequence |
| Protein sequence |
