AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060904

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060904.t1 MTR_1g013170 96.310 271 10 0 2 272 1 271 1.30e-170 482
MS.gene060904.t1 MTR_7g117705 80.524 267 50 2 2 267 1 266 4.12e-142 408
MS.gene060904.t1 MTR_1g013180 80.443 271 46 1 2 272 152 415 6.82e-142 415
MS.gene060904.t1 MTR_3g106220 69.091 275 78 3 3 272 5 277 3.56e-123 366
MS.gene060904.t1 MTR_2g450070 58.696 276 102 3 4 270 16 288 2.01e-98 303
MS.gene060904.t1 MTR_2g450070 58.696 276 102 3 4 270 16 288 3.08e-98 303
MS.gene060904.t1 MTR_4g121020 53.169 284 100 4 3 272 28 292 2.81e-94 293
MS.gene060904.t1 MTR_4g121020 53.169 284 100 4 3 272 28 292 2.97e-94 293
MS.gene060904.t1 MTR_2g034960 49.650 286 120 2 1 276 24 295 4.84e-93 288
MS.gene060904.t1 MTR_1g032570 53.846 260 115 2 5 260 10 268 5.02e-91 283
MS.gene060904.t1 MTR_4g131580 56.371 259 106 3 6 264 17 268 7.24e-89 277
MS.gene060904.t1 MTR_4g131600 56.371 259 105 4 1 258 15 266 1.71e-88 275
MS.gene060904.t1 MTR_8g079940 56.863 255 106 2 4 257 23 274 1.72e-88 274
MS.gene060904.t1 MTR_1g013160 93.243 148 10 0 68 215 1 148 3.97e-86 255
MS.gene060904.t1 MTR_4g131570 52.985 268 124 2 6 272 17 283 4.27e-86 269
MS.gene060904.t1 MTR_3g086100 51.254 279 127 3 6 280 13 286 1.26e-84 265
MS.gene060904.t1 MTR_3g102590 51.292 271 75 4 3 270 5 221 5.05e-76 232
MS.gene060904.t1 MTR_3g102590 53.750 240 58 2 3 241 5 192 6.85e-71 218
MS.gene060904.t1 MTR_1g032570 53.000 200 89 2 65 260 1 199 5.07e-64 211
MS.gene060904.t1 MTR_3g102600 46.988 249 70 3 2 250 4 190 6.97e-60 191
MS.gene060904.t1 MTR_3g086100 48.636 220 104 3 65 280 1 215 2.66e-58 194
MS.gene060904.t1 MTR_4g021760 38.406 276 139 5 6 280 11 256 1.02e-56 185
MS.gene060904.t1 MTR_3g102600 46.058 241 68 3 3 243 5 183 8.30e-56 179
MS.gene060904.t1 MTR_4g021790 37.407 270 140 5 3 272 7 247 2.16e-50 168
MS.gene060904.t1 MTR_4g021855 37.652 247 126 3 3 249 7 225 2.95e-50 168
MS.gene060904.t1 MTR_4g021845 38.912 239 118 4 3 241 7 217 5.05e-49 162
MS.gene060904.t1 MTR_4g121020 44.554 202 79 4 85 272 6 188 1.94e-47 167
MS.gene060904.t1 MTR_0450s0040 37.344 241 130 3 10 250 8 227 8.40e-45 155
MS.gene060904.t1 MTR_8g105600 36.000 275 147 7 6 272 9 262 4.64e-40 147
MS.gene060904.t1 MTR_3g102600 41.872 203 56 3 48 250 1 141 1.32e-39 137
MS.gene060904.t1 MTR_6g045327 33.878 245 132 3 5 248 13 228 5.87e-38 138
MS.gene060904.t1 MTR_7g026400 34.855 241 127 3 33 266 14 231 1.98e-37 140
MS.gene060904.t1 MTR_3g102600 40.306 196 55 3 48 243 1 134 1.27e-35 125
MS.gene060904.t1 MTR_5g055260 33.603 247 109 7 3 249 7 198 2.46e-32 120
MS.gene060904.t1 MTR_8g032710 30.040 253 159 5 9 244 20 271 6.89e-29 115
MS.gene060904.t1 MTR_4g064730 47.788 113 53 2 157 263 94 206 3.09e-23 97.4
MS.gene060904.t1 MTR_3g092780 34.532 139 86 2 10 144 56 193 3.30e-23 99.8
MS.gene060904.t1 MTR_6g016850 36.434 129 79 2 2 128 5 132 3.99e-23 94.0
MS.gene060904.t1 MTR_3g092780 34.532 139 86 2 10 144 56 193 4.06e-23 99.8
MS.gene060904.t1 MTR_4g023980 32.530 166 85 3 53 218 36 174 1.07e-22 92.8
MS.gene060904.t1 MTR_8g024260 34.524 168 96 3 12 165 38 205 1.23e-22 98.2
MS.gene060904.t1 MTR_3g092780 37.008 127 75 2 10 132 56 181 3.30e-22 97.1
MS.gene060904.t1 MTR_3g092780 37.008 127 75 2 10 132 56 181 3.61e-22 97.1
MS.gene060904.t1 MTR_7g118260 35.484 155 85 4 3 145 5 156 1.93e-21 94.7
MS.gene060904.t1 MTR_7g118260 30.366 191 118 4 3 181 5 192 1.01e-20 92.4
MS.gene060904.t1 MTR_7g089010 53.846 78 36 0 173 250 72 149 2.90e-20 89.0
MS.gene060904.t1 MTR_1g067110 36.885 122 74 2 10 128 85 206 4.99e-20 90.5
MS.gene060904.t1 MTR_1g067110 36.885 122 74 2 10 128 85 206 5.33e-20 90.5
MS.gene060904.t1 MTR_4g061360 36.885 122 74 1 10 128 89 210 1.05e-19 89.7
MS.gene060904.t1 MTR_8g093040 29.098 244 130 4 5 248 13 213 1.23e-19 87.0
MS.gene060904.t1 MTR_6g453220 77.358 53 12 0 208 260 379 431 1.95e-19 88.6
MS.gene060904.t1 MTR_3g037390 33.871 124 79 1 10 130 24 147 1.21e-18 86.3
MS.gene060904.t1 MTR_5g014040 52.703 74 34 1 185 257 304 377 2.62e-17 82.4
MS.gene060904.t1 MTR_5g014040 35.200 125 75 3 6 128 14 134 2.02e-12 67.8
MS.gene060904.t1 MTR_4g108880 32.258 124 81 1 10 130 33 156 4.10e-17 82.0
MS.gene060904.t1 MTR_4g098870 55.072 69 30 1 190 257 308 376 2.37e-16 79.7
MS.gene060904.t1 MTR_4g098870 32.258 124 80 2 6 128 14 134 4.48e-11 63.5
MS.gene060904.t1 MTR_4g098870 55.072 69 30 1 190 257 328 396 2.51e-16 79.3
MS.gene060904.t1 MTR_4g098870 32.258 124 80 2 6 128 34 154 4.64e-11 63.5
MS.gene060904.t1 MTR_4g098870 55.072 69 30 1 190 257 308 376 2.54e-16 79.3
MS.gene060904.t1 MTR_4g098870 32.258 124 80 2 6 128 14 134 5.92e-11 63.2
MS.gene060904.t1 MTR_4g098870 55.072 69 30 1 190 257 347 415 2.68e-16 79.3
MS.gene060904.t1 MTR_4g098870 32.258 124 80 2 6 128 53 173 5.71e-11 63.5
MS.gene060904.t1 MTR_4g098870 55.072 69 30 1 190 257 328 396 2.80e-16 79.3
MS.gene060904.t1 MTR_4g098870 32.258 124 80 2 6 128 34 154 5.97e-11 63.2
MS.gene060904.t1 MTR_5g014040 49.367 79 34 2 185 257 264 342 2.90e-15 76.3
MS.gene060904.t1 MTR_5g014040 49.367 79 34 2 185 257 304 382 3.16e-15 76.3
MS.gene060904.t1 MTR_5g014040 35.200 125 75 3 6 128 14 134 2.00e-12 67.8
MS.gene060904.t1 MTR_4g078810 55.357 56 25 0 189 244 155 210 3.26e-15 75.9
MS.gene060904.t1 MTR_2g090675 55.357 56 25 0 189 244 158 213 7.85e-15 74.7
MS.gene060904.t1 MTR_4g086835 50.746 67 33 0 187 253 269 335 8.09e-15 74.7
MS.gene060904.t1 MTR_1g093080 41.379 87 50 1 178 263 197 283 3.78e-14 72.4
MS.gene060904.t1 MTR_4g113140 52.857 70 31 2 183 251 227 295 6.20e-14 72.0
MS.gene060904.t1 MTR_5g017980 51.613 62 30 0 189 250 189 250 9.24e-14 70.9
MS.gene060904.t1 MTR_5g017980 51.613 62 30 0 189 250 190 251 9.40e-14 70.9
MS.gene060904.t1 MTR_7g115530 64.286 56 19 1 190 244 216 271 1.15e-13 70.5
MS.gene060904.t1 MTR_7g115530 64.286 56 19 1 190 244 216 271 1.62e-13 70.1
MS.gene060904.t1 MTR_7g115530 54.286 70 31 1 190 258 216 285 1.88e-13 70.1
MS.gene060904.t1 MTR_1g080330 60.345 58 22 1 188 244 171 228 2.52e-13 69.3
MS.gene060904.t1 MTR_7g115530 64.286 56 19 1 190 244 216 271 2.65e-13 70.1
MS.gene060904.t1 MTR_4g086835 50.000 68 33 1 187 253 269 336 2.93e-13 70.1
MS.gene060904.t1 MTR_1g080330 60.345 58 22 1 188 244 171 228 3.57e-13 69.7
MS.gene060904.t1 MTR_7g098250 52.941 68 31 1 188 254 258 325 8.08e-13 68.9
MS.gene060904.t1 MTR_2g023580 47.826 92 40 4 191 275 54 144 8.64e-13 67.0
MS.gene060904.t1 MTR_5g054300 43.590 78 43 1 173 249 179 256 1.66e-12 67.4
MS.gene060904.t1 MTR_5g017980 50.794 63 30 1 189 250 190 252 2.00e-12 67.0
MS.gene060904.t1 MTR_1g090670 55.932 59 25 1 188 245 40 98 2.90e-12 66.2
MS.gene060904.t1 MTR_3g093860 34.752 141 68 6 1 123 12 146 3.12e-12 63.9
MS.gene060904.t1 MTR_6g088950 30.645 124 85 1 8 130 5 128 4.76e-12 64.3
MS.gene060904.t1 MTR_7g115530 62.963 54 19 1 190 242 216 269 6.98e-12 65.1
MS.gene060904.t1 MTR_6g032990 54.545 55 24 1 192 245 42 96 7.20e-12 65.9
MS.gene060904.t1 MTR_2g086450 54.545 55 24 1 192 245 45 99 8.01e-12 65.5
MS.gene060904.t1 MTR_7g490310 30.216 139 76 3 12 129 11 149 9.82e-12 63.9
MS.gene060904.t1 MTR_5g027440 56.364 55 23 1 192 245 25 79 1.52e-11 63.9
MS.gene060904.t1 MTR_8g038620 28.986 138 78 3 12 129 11 148 1.74e-11 62.4
MS.gene060904.t1 MTR_5g041350 50.909 55 26 1 192 245 15 69 5.23e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060904.t1 AT2G25180 63.953 258 87 3 4 260 11 263 2.25e-106 322
MS.gene060904.t1 AT4G31920 58.803 284 95 5 4 286 11 273 1.53e-102 310
MS.gene060904.t1 AT3G16857 49.477 287 127 2 4 272 31 317 5.29e-91 284
MS.gene060904.t1 AT3G16857 49.477 287 127 2 4 272 31 317 1.07e-90 284
MS.gene060904.t1 AT5G58080 55.396 278 98 5 4 272 12 272 1.01e-83 264
MS.gene060904.t1 AT1G67710 54.150 253 109 3 7 255 8 257 4.93e-80 251
MS.gene060904.t1 AT2G01760 47.138 297 138 5 2 283 3 295 5.42e-79 245
MS.gene060904.t1 AT3G16857 48.780 246 108 2 45 272 2 247 8.26e-74 238
MS.gene060904.t1 AT2G01760 43.077 325 138 6 2 283 3 323 6.96e-73 230
MS.gene060904.t1 AT1G67710 52.308 195 86 3 65 255 1 192 8.87e-54 182
MS.gene060904.t1 AT5G49240 38.430 242 146 2 1 241 33 272 1.48e-51 171
MS.gene060904.t1 AT2G01760 44.017 234 112 5 65 283 1 230 3.45e-50 169
MS.gene060904.t1 AT3G62670 34.628 309 159 9 11 293 42 333 1.16e-46 162
MS.gene060904.t1 AT1G67710 46.857 175 86 3 85 255 12 183 7.99e-39 142
MS.gene060904.t1 AT1G49190 31.206 282 175 4 5 275 29 302 2.23e-35 132
MS.gene060904.t1 AT5G07210 31.047 277 149 7 8 251 14 281 3.75e-33 128
MS.gene060904.t1 AT5G07210 30.175 285 149 8 8 251 14 289 1.32e-30 121
MS.gene060904.t1 AT2G27070 29.577 284 145 7 8 250 14 283 2.39e-28 114
MS.gene060904.t1 AT2G46790 39.837 123 69 2 10 128 37 158 5.82e-25 103
MS.gene060904.t1 AT2G46790 39.837 123 69 2 10 128 37 158 6.36e-25 104
MS.gene060904.t1 AT5G59570 48.214 112 50 2 171 279 120 226 9.42e-24 98.6
MS.gene060904.t1 AT5G59570 48.214 112 50 2 171 279 120 226 9.42e-24 98.6
MS.gene060904.t1 AT2G40970 41.525 118 69 0 168 285 81 198 1.60e-23 97.1
MS.gene060904.t1 AT1G49190 37.600 125 78 0 5 129 29 153 1.68e-23 100
MS.gene060904.t1 AT5G24470 36.719 128 76 2 8 131 48 174 2.46e-22 97.4
MS.gene060904.t1 AT3G46640 36.420 162 83 4 105 250 45 202 4.41e-22 94.7
MS.gene060904.t1 AT3G46640 37.107 159 86 4 105 250 45 202 4.53e-22 94.4
MS.gene060904.t1 AT3G46640 37.107 159 86 4 105 250 45 202 4.53e-22 94.4
MS.gene060904.t1 AT5G02810 38.525 122 72 1 10 128 78 199 2.80e-21 94.4
MS.gene060904.t1 AT5G05090 53.165 79 37 0 172 250 61 139 9.56e-21 89.7
MS.gene060904.t1 AT1G68210 25.632 277 177 3 10 258 13 288 1.27e-20 92.4
MS.gene060904.t1 AT5G60100 37.795 127 75 2 6 128 59 185 1.44e-20 91.3
MS.gene060904.t1 AT5G60100 37.795 127 75 2 6 128 59 185 1.44e-20 91.3
MS.gene060904.t1 AT5G60100 37.795 127 75 2 6 128 59 185 1.44e-20 91.3
MS.gene060904.t1 AT5G60100 37.795 127 75 2 6 128 59 185 1.59e-20 91.7
MS.gene060904.t1 AT5G60100 37.795 127 75 2 6 128 59 185 1.59e-20 91.7
MS.gene060904.t1 AT5G60100 37.795 127 75 2 6 128 59 185 1.59e-20 91.7
MS.gene060904.t1 AT3G10760 65.000 60 21 0 191 250 104 163 2.52e-20 89.7
MS.gene060904.t1 AT5G60100 37.795 127 75 2 6 128 59 185 2.64e-20 91.3
MS.gene060904.t1 AT5G44190 40.984 122 64 3 184 299 139 258 6.76e-19 86.3
MS.gene060904.t1 AT5G61380 33.871 124 79 1 10 130 19 142 8.23e-19 87.0
MS.gene060904.t1 AT5G44190 40.984 122 64 3 184 299 139 258 8.49e-19 85.9
MS.gene060904.t1 AT2G20570 44.330 97 43 1 154 250 126 211 1.91e-16 79.3
MS.gene060904.t1 AT2G20570 61.111 54 21 0 197 250 174 227 4.26e-15 75.5
MS.gene060904.t1 AT4G18020 55.932 59 26 0 197 255 301 359 1.22e-14 74.3
MS.gene060904.t1 AT4G18020 55.932 59 26 0 197 255 301 359 1.22e-14 74.3
MS.gene060904.t1 AT4G18020 55.932 59 26 0 197 255 301 359 1.22e-14 74.3
MS.gene060904.t1 AT4G18020 55.932 59 26 0 197 255 301 359 1.22e-14 74.3
MS.gene060904.t1 AT4G18020 55.932 59 26 0 197 255 301 359 1.22e-14 74.3
MS.gene060904.t1 AT4G18020 55.932 59 26 0 197 255 301 359 1.22e-14 74.3
MS.gene060904.t1 AT4G18020 55.932 59 26 0 197 255 301 359 1.38e-14 74.3
MS.gene060904.t1 AT4G18020 55.932 59 26 0 197 255 301 359 1.38e-14 74.3
MS.gene060904.t1 AT4G18020 55.932 59 26 0 197 255 301 359 1.38e-14 74.3
MS.gene060904.t1 AT4G18020 55.932 59 26 0 197 255 301 359 1.38e-14 74.3
MS.gene060904.t1 AT4G18020 55.932 59 26 0 197 255 301 359 1.38e-14 74.3
MS.gene060904.t1 AT2G03500 53.125 64 29 1 189 251 230 293 2.85e-13 70.1
MS.gene060904.t1 AT3G57040 28.395 162 94 2 12 151 11 172 8.75e-13 67.0
MS.gene060904.t1 AT3G57040 28.395 162 94 2 12 151 11 172 8.75e-13 67.0
MS.gene060904.t1 AT5G45580 43.182 88 47 2 182 266 11 98 3.64e-12 65.9
MS.gene060904.t1 AT3G04030 54.545 55 24 1 192 245 45 99 6.95e-12 65.9
MS.gene060904.t1 AT3G04030 54.545 55 24 1 192 245 45 99 7.34e-12 65.9
MS.gene060904.t1 AT3G56380 31.298 131 76 2 10 126 20 150 8.12e-12 62.8
MS.gene060904.t1 AT5G45580 44.737 76 41 1 192 266 7 82 1.05e-11 64.3
MS.gene060904.t1 AT3G04030 51.852 54 26 0 192 245 45 98 1.10e-11 65.1
MS.gene060904.t1 AT1G25550 51.667 60 28 1 191 249 208 267 1.16e-11 65.1
MS.gene060904.t1 AT3G56380 31.538 130 76 2 10 126 20 149 1.53e-11 62.0
MS.gene060904.t1 AT4G13640 39.048 105 54 3 188 282 33 137 1.97e-11 63.9
MS.gene060904.t1 AT4G13640 39.048 105 54 3 188 282 33 137 2.01e-11 63.9
MS.gene060904.t1 AT1G13300 47.692 65 33 1 186 249 175 239 2.45e-11 63.9
MS.gene060904.t1 AT2G40670 32.331 133 74 2 10 126 29 161 2.52e-11 61.6
MS.gene060904.t1 AT3G24120 51.613 62 29 1 188 248 37 98 2.55e-11 63.5
MS.gene060904.t1 AT3G24120 51.613 62 29 1 188 248 37 98 2.63e-11 63.5
MS.gene060904.t1 AT1G49560 45.833 72 38 1 187 257 187 258 4.22e-11 63.2
MS.gene060904.t1 AT4G37180 49.180 61 30 1 190 249 208 268 5.29e-11 63.2
MS.gene060904.t1 AT2G40670 32.576 132 74 2 10 126 29 160 5.65e-11 60.5
MS.gene060904.t1 AT4G37180 49.180 61 30 1 190 249 215 275 6.28e-11 62.8

Find 60 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGAACATGTCTCAAGATTT+TGG 0.179210 1.2:-8511012 MS.gene060904:CDS
ATTCCTTTCATCACCATCTT+TGG 0.256348 1.2:+8510162 None:intergenic
CCATTTCCACTATCAGAATT+AGG 0.318838 1.2:+8510024 None:intergenic
ATATGTGAGACTGAGAAGTA+TGG 0.327860 1.2:+8509088 None:intergenic
ATGAATGATCAGTTTCCTAT+TGG 0.330551 1.2:-8511062 MS.gene060904:CDS
CCTAATTCTGATAGTGGAAA+TGG 0.330629 1.2:-8510024 MS.gene060904:CDS
TTTAAGCTTCTTGAGCTTGT+TGG 0.375182 1.2:-8510317 MS.gene060904:CDS
AAAGGTGGCCAGCCATCTTC+AGG 0.376644 1.2:-8509534 MS.gene060904:intron
ACATGGTGCGTGTGATTATC+TGG 0.387305 1.2:-8510136 MS.gene060904:CDS
TAAAAGAGGTACAGATCATT+TGG 0.388547 1.2:-8510098 MS.gene060904:CDS
TATGAACATTGCTGATTACT+AGG 0.423694 1.2:+8510357 None:intergenic
TACCATGGTATTGACACCTT+TGG 0.431203 1.2:+8510979 None:intergenic
AGAATGTCACTGGGAAAGAT+TGG 0.434197 1.2:+8509122 None:intergenic
TCATTTGGCAGCATGTAATC+AGG 0.452747 1.2:-8510083 MS.gene060904:CDS
GACGCGCCTAATTCTGATAG+TGG 0.455329 1.2:-8510030 MS.gene060904:CDS
AGAAGAAGCCTCGAACTGTT+TGG 0.455763 1.2:-8509873 MS.gene060904:CDS
TGAGCTTGTTGGACTTGAGA+TGG 0.457732 1.2:-8510306 MS.gene060904:CDS
GCAAGAACTCGCATTCCAAT+AGG 0.458411 1.2:+8511047 None:intergenic
AGCTTAAATCCATCCATGTC+TGG 0.471845 1.2:+8510332 None:intergenic
GTTGTTGCGGTCAATCAATT+AGG 0.476132 1.2:-8509826 MS.gene060904:CDS
GGGAAAGATTCGGCTGGGAC+AGG 0.479650 1.2:-8510003 MS.gene060904:CDS
CTAATTCTGATAGTGGAAAT+GGG 0.482228 1.2:-8510023 MS.gene060904:CDS
TGAAAATCTGACAAGGGAAA+AGG 0.489796 1.2:-8509552 MS.gene060904:CDS
TAGCTAAGGCTGCTTCCATA+TGG 0.490232 1.2:+8509176 None:intergenic
CACTGCTCAAAACGCGATAA+CGG 0.499559 1.2:-8510417 MS.gene060904:CDS
TAGTGGAAATGGGAAAGATT+CGG 0.502507 1.2:-8510013 MS.gene060904:CDS
GTCAATCAATTAGGCATTGA+CGG 0.502566 1.2:-8509817 MS.gene060904:intron
GGGTGGGAAAAGAATGTCAC+TGG 0.506615 1.2:+8509112 None:intergenic
AGCATAAAAGTACCTGAAGA+TGG 0.509866 1.2:+8509522 None:intergenic
ACATCGCAAGTTTGTTGTTG+CGG 0.516543 1.2:-8509839 MS.gene060904:CDS
TATGTGAGACTGAGAAGTAT+GGG 0.518755 1.2:+8509089 None:intergenic
GAAACCTGTTAGACTAAAAG+AGG 0.521944 1.2:-8510112 MS.gene060904:CDS
TGACAGTGAGGAAGAAAATG+AGG 0.529313 1.2:-8509926 MS.gene060904:CDS
GGAAATGGGAAAGATTCGGC+TGG 0.530128 1.2:-8510009 MS.gene060904:CDS
TGCATCTGAACTACTAGCTA+AGG 0.539318 1.2:+8509162 None:intergenic
TATTGGAATGCGAGTTCTTG+CGG 0.540178 1.2:-8511045 MS.gene060904:CDS
TTGTATAACTTACTTATGAC+AGG 0.544810 1.2:+8510278 None:intergenic
TAAAAGTACCTGAAGATGGC+TGG 0.546379 1.2:+8509526 None:intergenic
TCAGTGGACCAAACAGTTCG+AGG 0.556377 1.2:+8509865 None:intergenic
CAATGTTCATATGCCAGACA+TGG 0.556391 1.2:-8510345 MS.gene060904:CDS
AGATTGGCTGCTGAGTTGAT+AGG 0.561182 1.2:+8509138 None:intergenic
TAAGAATGAAGATGACAGTG+AGG 0.570920 1.2:-8509938 MS.gene060904:CDS
GGAAGACAGACAAGACCATA+TGG 0.582020 1.2:-8509191 MS.gene060904:CDS
GTTCATATGCCAGACATGGA+TGG 0.584384 1.2:-8510341 MS.gene060904:CDS
ATGTGAGACTGAGAAGTATG+GGG 0.595182 1.2:+8509090 None:intergenic
TGCGAATGATGATCCAAAGA+TGG 0.615837 1.2:-8510175 MS.gene060904:CDS
GGTCTTGTCTGTCTTCCGCA+CGG 0.622559 1.2:+8509197 None:intergenic
GAAATGGGAAAGATTCGGCT+GGG 0.627002 1.2:-8510008 MS.gene060904:CDS
AAATCTGACAAGGGAAAAGG+TGG 0.629315 1.2:-8509549 MS.gene060904:CDS
GATCCAAAGATGGTGATGAA+AGG 0.637771 1.2:-8510165 MS.gene060904:CDS
ATAACTTACTTATGACAGGT+AGG 0.639228 1.2:+8510282 None:intergenic
TGTGAGACTGAGAAGTATGG+GGG 0.643481 1.2:+8509091 None:intergenic
CTCCAAAGGTGTCAATACCA+TGG 0.650196 1.2:-8510981 MS.gene060904:intron
AAACTTGCGATGTAAATCAG+TGG 0.662856 1.2:+8509849 None:intergenic
GTGAGACTGAGAAGTATGGG+GGG 0.686210 1.2:+8509092 None:intergenic
GAATGTTGAAAATCTGACAA+GGG 0.692411 1.2:-8509558 MS.gene060904:CDS
TGAATGTTGAAAATCTGACA+AGG 0.698854 1.2:-8509559 MS.gene060904:CDS
GGTGGGAAAAGAATGTCACT+GGG 0.701317 1.2:+8509113 None:intergenic
GTGATGAAAGGAATTGAACA+TGG 0.742148 1.2:-8510153 MS.gene060904:CDS
TCTACAAAGAATTAGCCGTG+CGG 0.798557 1.2:-8509212 MS.gene060904:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGATATTAATTTTAGTTA+TGG - chr1.2:8509815-8509834 MS.gene060904:intron 10.0%
!!! AATTTTATTTGATATTGTTT+AGG - chr1.2:8509588-8509607 MS.gene060904:CDS 10.0%
!!! TATTTTGAAATATTAAAGAT+TGG - chr1.2:8509360-8509379 MS.gene060904:intron 10.0%
!! AAAAAACAAGATTAAAATCA+AGG + chr1.2:8509387-8509406 None:intergenic 15.0%
!! AAGTATATATACAAAAAAGA+AGG + chr1.2:8509081-8509100 None:intergenic 15.0%
!!! ATTATTATTTTCAATGAAGT+TGG - chr1.2:8509481-8509500 MS.gene060904:intron 15.0%
!!! CAAAAAAATAGTGATTTTTA+AGG + chr1.2:8509180-8509199 None:intergenic 15.0%
!! AAATCTAATGATATATAGCT+AGG - chr1.2:8509415-8509434 MS.gene060904:intron 20.0%
!! AATCTAATGATATATAGCTA+GGG - chr1.2:8509416-8509435 MS.gene060904:intron 20.0%
!! TATCGATAATATAAGATTGA+AGG + chr1.2:8510027-8510046 None:intergenic 20.0%
! ATGAAAAACCATCTAGAAAA+AGG - chr1.2:8510311-8510330 MS.gene060904:CDS 25.0%
! CATGATATTAATCTCAACAT+TGG + chr1.2:8510527-8510546 None:intergenic 25.0%
! TTGTATAACTTACTTATGAC+AGG + chr1.2:8510002-8510021 None:intergenic 25.0%
!! TAATTGATTTCTACAAGCAT+TGG + chr1.2:8509557-8509576 None:intergenic 25.0%
!!! ATCAGTTCAAGAATCTTTTT+TGG + chr1.2:8510699-8510718 None:intergenic 25.0%
AAAACCATCTAGAAAAAGGA+AGG - chr1.2:8510315-8510334 MS.gene060904:CDS 30.0%
AATCGGAAAAATTCATAGCA+TGG + chr1.2:8510627-8510646 None:intergenic 30.0%
ATAACTTACTTATGACAGGT+AGG + chr1.2:8509998-8510017 None:intergenic 30.0%
ATGAATGATCAGTTTCCTAT+TGG - chr1.2:8509215-8509234 MS.gene060904:CDS 30.0%
GAATGTTGAAAATCTGACAA+GGG - chr1.2:8510719-8510738 MS.gene060904:intron 30.0%
TGAATGTTGAAAATCTGACA+AGG - chr1.2:8510718-8510737 MS.gene060904:intron 30.0%
TGAGAAAAACACTTCAGAAT+CGG + chr1.2:8510644-8510663 None:intergenic 30.0%
TTCTCGATTCTTTCAAAGAT+TGG - chr1.2:8509641-8509660 MS.gene060904:intron 30.0%
! ATGATATATAGCTAGGGTTT+TGG - chr1.2:8509422-8509441 MS.gene060904:intron 30.0%
! GCTAAACATACGTATTTTTC+GGG + chr1.2:8510605-8510624 None:intergenic 30.0%
! GGCTAAACATACGTATTTTT+CGG + chr1.2:8510606-8510625 None:intergenic 30.0%
! TAAAAGAGGTACAGATCATT+TGG - chr1.2:8510179-8510198 MS.gene060904:CDS 30.0%
! TAGAACATGTCTCAAGATTT+TGG - chr1.2:8509265-8509284 MS.gene060904:intron 30.0%
! TATGAACATTGCTGATTACT+AGG + chr1.2:8509923-8509942 None:intergenic 30.0%
! TTATCCTTCCTTTTTCTAGA+TGG + chr1.2:8510322-8510341 None:intergenic 30.0%
!! CTAATTCTGATAGTGGAAAT+GGG - chr1.2:8510254-8510273 MS.gene060904:intron 30.0%
!!! TTTTTCGGGAAGAATATTTG+AGG + chr1.2:8510591-8510610 None:intergenic 30.0%
AAACTTGCGATGTAAATCAG+TGG + chr1.2:8510431-8510450 None:intergenic 35.0%
ACGTAAGATCACCAAAGATT+AGG + chr1.2:8509717-8509736 None:intergenic 35.0%
AGTTGTTATCTTACTTGCAG+AGG - chr1.2:8510668-8510687 MS.gene060904:intron 35.0%
ATATGTGAGACTGAGAAGTA+TGG + chr1.2:8511192-8511211 None:intergenic 35.0%
ATTCCTTTCATCACCATCTT+TGG + chr1.2:8510118-8510137 None:intergenic 35.0%
CCATTTCCACTATCAGAATT+AGG + chr1.2:8510256-8510275 None:intergenic 35.0%
GAAACCTGTTAGACTAAAAG+AGG - chr1.2:8510165-8510184 MS.gene060904:CDS 35.0%
GTGATGAAAGGAATTGAACA+TGG - chr1.2:8510124-8510143 MS.gene060904:CDS 35.0%
TAAGAATGAAGATGACAGTG+AGG - chr1.2:8510339-8510358 MS.gene060904:CDS 35.0%
TAGTGGAAATGGGAAAGATT+CGG - chr1.2:8510264-8510283 MS.gene060904:intron 35.0%
TGAAAATCTGACAAGGGAAA+AGG - chr1.2:8510725-8510744 MS.gene060904:intron 35.0%
TTTAAGCTTCTTGAGCTTGT+TGG - chr1.2:8509960-8509979 MS.gene060904:CDS 35.0%
! TATGTGAGACTGAGAAGTAT+GGG + chr1.2:8511191-8511210 None:intergenic 35.0%
! TCTTTTGTGTGCCTAATCTT+TGG - chr1.2:8509703-8509722 MS.gene060904:intron 35.0%
! TGTACCTCTTTTAGTCTAAC+AGG + chr1.2:8510172-8510191 None:intergenic 35.0%
!! AGCATAAAAGTACCTGAAGA+TGG + chr1.2:8510758-8510777 None:intergenic 35.0%
!! CCTAATTCTGATAGTGGAAA+TGG - chr1.2:8510253-8510272 MS.gene060904:intron 35.0%
!! GTCAATCAATTAGGCATTGA+CGG - chr1.2:8510460-8510479 MS.gene060904:intron 35.0%
!! TTTTGGAGAAACTTCTCCAA+AGG - chr1.2:8509282-8509301 MS.gene060904:intron 35.0%
AAATCTGACAAGGGAAAAGG+TGG - chr1.2:8510728-8510747 MS.gene060904:intron 40.0%
AATCTGCGCATTGAAGAAAG+AGG + chr1.2:8510496-8510515 None:intergenic 40.0%
AGAATGTCACTGGGAAAGAT+TGG + chr1.2:8511158-8511177 None:intergenic 40.0%
AGCTTAAATCCATCCATGTC+TGG + chr1.2:8509948-8509967 None:intergenic 40.0%
ATCTGCGCATTGAAGAAAGA+GGG + chr1.2:8510495-8510514 None:intergenic 40.0%
ATTAACAGTGTCCTTGTGTC+AGG + chr1.2:8510828-8510847 None:intergenic 40.0%
CAATGTTCATATGCCAGACA+TGG - chr1.2:8509932-8509951 MS.gene060904:CDS 40.0%
GTTGTTGCGGTCAATCAATT+AGG - chr1.2:8510451-8510470 MS.gene060904:intron 40.0%
TATTGGAATGCGAGTTCTTG+CGG - chr1.2:8509232-8509251 MS.gene060904:intron 40.0%
TCATTTGGCAGCATGTAATC+AGG - chr1.2:8510194-8510213 MS.gene060904:intron 40.0%
TCTACAAAGAATTAGCCGTG+CGG - chr1.2:8511065-8511084 MS.gene060904:CDS 40.0%
TGACACAAGGACACTGTTAA+TGG - chr1.2:8510827-8510846 MS.gene060904:intron 40.0%
TGACAGTGAGGAAGAAAATG+AGG - chr1.2:8510351-8510370 MS.gene060904:CDS 40.0%
TGCATCTGAACTACTAGCTA+AGG + chr1.2:8511118-8511137 None:intergenic 40.0%
TGCGAATGATGATCCAAAGA+TGG - chr1.2:8510102-8510121 MS.gene060904:CDS 40.0%
TGTCTTTGTCGTGTTAGTGT+CGG - chr1.2:8510945-8510964 MS.gene060904:intron 40.0%
TGTTGATGTCGTGTTAGTGT+CGG - chr1.2:8510885-8510904 MS.gene060904:intron 40.0%
! ATGTGAGACTGAGAAGTATG+GGG + chr1.2:8511190-8511209 None:intergenic 40.0%
! GATCCAAAGATGGTGATGAA+AGG - chr1.2:8510112-8510131 MS.gene060904:CDS 40.0%
!! ACATCGCAAGTTTGTTGTTG+CGG - chr1.2:8510438-8510457 MS.gene060904:intron 40.0%
!! GGTGATCTTACGTTGTTTCT+TGG - chr1.2:8509724-8509743 MS.gene060904:intron 40.0%
!! GTTGATGATGAAGCATACCA+TGG + chr1.2:8509316-8509335 None:intergenic 40.0%
!! TAAAAGTACCTGAAGATGGC+TGG + chr1.2:8510754-8510773 None:intergenic 40.0%
!! TACCATGGTATTGACACCTT+TGG + chr1.2:8509301-8509320 None:intergenic 40.0%
!!! CTAGGGTTTTGGCTAAGTTT+TGG - chr1.2:8509433-8509452 MS.gene060904:intron 40.0%
ACATGGTGCGTGTGATTATC+TGG - chr1.2:8510141-8510160 MS.gene060904:CDS 45.0%
AGAAGAAGCCTCGAACTGTT+TGG - chr1.2:8510404-8510423 MS.gene060904:CDS 45.0%
CACTGCTCAAAACGCGATAA+CGG - chr1.2:8509860-8509879 MS.gene060904:CDS 45.0%
CCTATAATGAGCCTGACACA+AGG - chr1.2:8510814-8510833 MS.gene060904:intron 45.0%
CCTTGTGTCAGGCTCATTAT+AGG + chr1.2:8510817-8510836 None:intergenic 45.0%
GAAATGGGAAAGATTCGGCT+GGG - chr1.2:8510269-8510288 MS.gene060904:intron 45.0%
GCAAGAACTCGCATTCCAAT+AGG + chr1.2:8509233-8509252 None:intergenic 45.0%
GGAAGACAGACAAGACCATA+TGG - chr1.2:8511086-8511105 MS.gene060904:intron 45.0%
GGTGGGAAAAGAATGTCACT+GGG + chr1.2:8511167-8511186 None:intergenic 45.0%
GTTCATATGCCAGACATGGA+TGG - chr1.2:8509936-8509955 MS.gene060904:CDS 45.0%
TAGCTAAGGCTGCTTCCATA+TGG + chr1.2:8511104-8511123 None:intergenic 45.0%
! TGTGAGACTGAGAAGTATGG+GGG + chr1.2:8511189-8511208 None:intergenic 45.0%
!! AGATTGGCTGCTGAGTTGAT+AGG + chr1.2:8511142-8511161 None:intergenic 45.0%
!! CTCCAAAGGTGTCAATACCA+TGG - chr1.2:8509296-8509315 MS.gene060904:intron 45.0%
!! TGAGCTTGTTGGACTTGAGA+TGG - chr1.2:8509971-8509990 MS.gene060904:CDS 45.0%
GACGCGCCTAATTCTGATAG+TGG - chr1.2:8510247-8510266 MS.gene060904:intron 50.0%
GGAAATGGGAAAGATTCGGC+TGG - chr1.2:8510268-8510287 MS.gene060904:intron 50.0%
GGGTGGGAAAAGAATGTCAC+TGG + chr1.2:8511168-8511187 None:intergenic 50.0%
TCAGTGGACCAAACAGTTCG+AGG + chr1.2:8510415-8510434 None:intergenic 50.0%
! GTGAGACTGAGAAGTATGGG+GGG + chr1.2:8511188-8511207 None:intergenic 50.0%
!! CGTTATCGCGTTTTGAGCAG+TGG + chr1.2:8509862-8509881 None:intergenic 50.0%
AAAGGTGGCCAGCCATCTTC+AGG - chr1.2:8510743-8510762 MS.gene060904:intron 55.0%
! AGACTGAGAAGTATGGGGGG+TGG + chr1.2:8511185-8511204 None:intergenic 55.0%
! GACTGAGAAGTATGGGGGGT+GGG + chr1.2:8511184-8511203 None:intergenic 55.0%
! GGTCTTGTCTGTCTTCCGCA+CGG + chr1.2:8511083-8511102 None:intergenic 55.0%
GGACGCAGAATGCACAGCGT+CGG - chr1.2:8510906-8510925 MS.gene060904:intron 60.0%
GGGAAAGATTCGGCTGGGAC+AGG - chr1.2:8510274-8510293 MS.gene060904:intron 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 8509072 8511227 8509072 ID=MS.gene060904
chr1.2 mRNA 8509072 8511227 8509072 ID=MS.gene060904.t1;Parent=MS.gene060904
chr1.2 exon 8511225 8511227 8511225 ID=MS.gene060904.t1.exon1;Parent=MS.gene060904.t1
chr1.2 CDS 8511225 8511227 8511225 ID=cds.MS.gene060904.t1;Parent=MS.gene060904.t1
chr1.2 exon 8510982 8511084 8510982 ID=MS.gene060904.t1.exon2;Parent=MS.gene060904.t1
chr1.2 CDS 8510982 8511084 8510982 ID=cds.MS.gene060904.t1;Parent=MS.gene060904.t1
chr1.2 exon 8510291 8510443 8510291 ID=MS.gene060904.t1.exon3;Parent=MS.gene060904.t1
chr1.2 CDS 8510291 8510443 8510291 ID=cds.MS.gene060904.t1;Parent=MS.gene060904.t1
chr1.2 exon 8509818 8510204 8509818 ID=MS.gene060904.t1.exon4;Parent=MS.gene060904.t1
chr1.2 CDS 8509818 8510204 8509818 ID=cds.MS.gene060904.t1;Parent=MS.gene060904.t1
chr1.2 exon 8509535 8509611 8509535 ID=MS.gene060904.t1.exon5;Parent=MS.gene060904.t1
chr1.2 CDS 8509535 8509611 8509535 ID=cds.MS.gene060904.t1;Parent=MS.gene060904.t1
chr1.2 exon 8509072 8509248 8509072 ID=MS.gene060904.t1.exon6;Parent=MS.gene060904.t1
chr1.2 CDS 8509072 8509248 8509072 ID=cds.MS.gene060904.t1;Parent=MS.gene060904.t1
Gene Sequence

>MS.gene060904

ATGGTACCTTCTTTTTTGTATATATACTTCATAACATTCTTTTGTCAATCATTCTATCTTTGCATTCATCACTTATTATTTTTCTCTTTGTCAACGAAAATTCTTCCTTAAAAATCACTATTTTTTTGCTTTGATTTCTCAAGATGAATGATCAGTTTCCTATTGGAATGCGAGTTCTTGCGGTTGATGATGATAGAACATGTCTCAAGATTTTGGAGAAACTTCTCCAAAGGTGTCAATACCATGGTATGCTTCATCATCAACATCATATTATTGTTGTTTTTGTTTTATTTTGAAATATTAAAGATTGGTCCTTGATTTTAATCTTGTTTTTTGATGTGACAAATCTAATGATATATAGCTAGGGTTTTGGCTAAGTTTTGGATACATATATTTATGCATATGTTAGATTATTATTTTCAATGAAGTTGGTTCATTTTGAATCAAATATCTATTTTTCAATATTATGTTCAACACTACATCCAATGCTTGTAGAAATCAATTAGATTAATTTTAAATTTTATTTGATATTGTTTAGGTTTAGACTAAATTATATTTGATCGATCACATTCTCGATTCTTTCAAAGATTGGACATGATTTTGCTTTTAAAGTTGCAATTTTTTTTAAATATCTTTTGTGTGCCTAATCTTTGGTGATCTTACGTTGTTTCTTGGATTTCAAAACATTTCGTAGATTTTATGTTCTGATTGTATATGCAGTGTATAATTTTTTTCTCTTTGAGAATGATATTAATTTTAGTTATGGTTTGTTAATTTGTTTCAGTAACCACTGCTCAAAACGCGATAACGGCGTTGAACTTGTTGAGAGAAAACAAAAGCAATTTTGACCTAGTAATCAGCAATGTTCATATGCCAGACATGGATGGATTTAAGCTTCTTGAGCTTGTTGGACTTGAGATGGACCTACCTGTCATAAGTAAGTTATACAATTCCTTCAATCTTATATTATCGATACATGTATAACAAATTATAGTATTTTGATTACGAAGTGTTTGATTTCAGTGTTTTCTGCGAATGATGATCCAAAGATGGTGATGAAAGGAATTGAACATGGTGCGTGTGATTATCTGGTGAAACCTGTTAGACTAAAAGAGGTACAGATCATTTGGCAGCATGTAATCAGGAAGAAGAAAACTAGCAAACGAAGCAATCATGACGCGCCTAATTCTGATAGTGGAAATGGGAAAGATTCGGCTGGGACAGGAAATTCTGATAAGAATGAAAAACCATCTAGAAAAAGGAAGGATAAGAATGAAGATGACAGTGAGGAAGAAAATGAGGATGATTATGACAATGATGATCAAACTGCGCAGAAGAAGCCTCGAACTGTTTGGTCCACTGATTTACATCGCAAGTTTGTTGTTGCGGTCAATCAATTAGGCATTGACGGTACGTTCTTCCCTCTTTCTTCAATGCGCAGATTATAACGATCCAATGTTGAGATTAATATCATGATATAAAAATAAAAATTCTACTTTGTATTATTTGAAGACTTCCTCAAATATTCTTCCCGAAAAATACGTATGTTTAGCCATGCTATGAATTTTTCCGATTCTGAAGTGTTTTTCTCATGATAGTTGTTATCTTACTTGCAGAGGCTGTACCAAAAAAGATTCTTGAACTGATGAATGTTGAAAATCTGACAAGGGAAAAGGTGGCCAGCCATCTTCAGGTACTTTTATGCTTTAGCATTATAAGTTACGAAGATATAGACACGTAAACCTATAATGAGCCTGACACAAGGACACTGTTAATGGTTTGATTGAATGAAGTAATTGAATTTAATCACATATGTTGATGTCGTGTTAGTGTCGGACGCAGAATGCACAGCGTCGGTGCTACACAAATTATCTGTCTTTGTCGTGTTAGTGTCGGACGCAGAATGCACAGCGTCGGTGCTACACAAATTATCTGTCTTTTCATTCAAGAAAACTTACTGTTTTTTAATTTTTCTGCAGAAGTATAGACTCTATCTACAAAGAATTAGCCGTGCGGAAGACAGACAAGACCATATGGAAGCAGCCTTAGCTAGTAGTTCAGATGCATCCTATCAACTCAGCAGCCAATCTTTCCCAGTGACATTCTTTTCCCACCCCCCATACTTCTCAGTCTCACATATCTTTCGTGATTTGTAA
Protein sequence

>MS.gene060904.t1

MMNDQFPIGMRVLAVDDDRTCLKILEKLLQRCQYHVTTAQNAITALNLLRENKSNFDLVISNVHMPDMDGFKLLELVGLEMDLPVIMFSANDDPKMVMKGIEHGACDYLVKPVRLKEVQIIWQHVIRKKKTSKRSNHDAPNSDSGNGKDSAGTGNSDKNEKPSRKRKDKNEDDSEEENEDDYDNDDQTAQKKPRTVWSTDLHRKFVVAVNQLGIDEAVPKKILELMNVENLTREKVASHLQKYRLYLQRISRAEDRQDHMEAALASSSDASYQLSSQSFPVTFFSHPPYFSVSHIFRDL