Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061770.t1 | RHN40044.1 | 68 | 253 | 58 | 8 | 1 | 248 | 1 | 235 | 1.40E-84 | 322.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061770.t1 | Q7M443 | 34.9 | 241 | 134 | 7 | 14 | 248 | 2 | 225 | 2.3e-27 | 124.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061770.t1 | A0A396GKD7 | 68.0 | 253 | 58 | 8 | 1 | 248 | 1 | 235 | 1.0e-84 | 322.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051860 | MS.gene061770 | 0.830519 | 2.81E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061770.t1 | MTR_0002s0040 | 56.757 | 259 | 83 | 7 | 1 | 248 | 1 | 241 | 5.50e-86 | 258 |
| MS.gene061770.t1 | MTR_0263s0020 | 56.757 | 259 | 83 | 7 | 1 | 248 | 1 | 241 | 4.12e-85 | 255 |
| MS.gene061770.t1 | MTR_1457s0010 | 66.310 | 187 | 42 | 6 | 65 | 248 | 1 | 169 | 2.84e-74 | 225 |
| MS.gene061770.t1 | MTR_0002s1060 | 52.326 | 258 | 91 | 10 | 4 | 248 | 6 | 244 | 5.06e-66 | 207 |
| MS.gene061770.t1 | MTR_0443s0040 | 40.856 | 257 | 111 | 10 | 14 | 247 | 16 | 254 | 7.23e-43 | 147 |
| MS.gene061770.t1 | MTR_0062s0090 | 40.726 | 248 | 114 | 8 | 14 | 244 | 4 | 235 | 3.54e-41 | 143 |
| MS.gene061770.t1 | MTR_0062s0040 | 40.726 | 248 | 114 | 8 | 14 | 244 | 4 | 235 | 3.54e-41 | 143 |
| MS.gene061770.t1 | MTR_0062s0060 | 40.726 | 248 | 114 | 8 | 14 | 244 | 4 | 235 | 3.54e-41 | 143 |
| MS.gene061770.t1 | MTR_0062s0020 | 39.200 | 250 | 120 | 6 | 14 | 247 | 4 | 237 | 3.20e-40 | 140 |
| MS.gene061770.t1 | MTR_6g079630 | 33.884 | 242 | 139 | 6 | 12 | 248 | 32 | 257 | 3.47e-31 | 117 |
| MS.gene061770.t1 | MTR_0062s0170 | 35.955 | 267 | 132 | 8 | 1 | 245 | 1 | 250 | 2.66e-30 | 115 |
| MS.gene061770.t1 | MTR_0062s0110 | 35.955 | 267 | 132 | 8 | 1 | 245 | 1 | 250 | 2.66e-30 | 115 |
| MS.gene061770.t1 | MTR_0062s0130 | 35.955 | 267 | 132 | 8 | 1 | 245 | 1 | 250 | 2.66e-30 | 115 |
| MS.gene061770.t1 | MTR_2g036240 | 35.955 | 267 | 132 | 8 | 1 | 245 | 1 | 250 | 2.66e-30 | 115 |
| MS.gene061770.t1 | MTR_2g036260 | 35.955 | 267 | 132 | 8 | 1 | 245 | 1 | 250 | 2.66e-30 | 115 |
| MS.gene061770.t1 | MTR_2g036290 | 35.955 | 267 | 132 | 8 | 1 | 245 | 1 | 250 | 2.66e-30 | 115 |
| MS.gene061770.t1 | MTR_8g046730 | 35.955 | 267 | 132 | 8 | 1 | 245 | 1 | 250 | 2.66e-30 | 115 |
| MS.gene061770.t1 | MTR_2g096030 | 34.167 | 240 | 137 | 7 | 14 | 248 | 31 | 254 | 8.10e-29 | 110 |
| MS.gene061770.t1 | MTR_2g096040 | 34.562 | 217 | 119 | 5 | 39 | 247 | 26 | 227 | 1.07e-26 | 104 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATATAAAAGATTTTGGGTA+TGG | - | 57516:21942-21961 | MS.gene061770:intergenic | 20.0% |
| !! | CAGGGAATATAAAAGATTTT+GGG | - | 57516:21947-21966 | MS.gene061770:intergenic | 25.0% |
| !! | TCAGGGAATATAAAAGATTT+TGG | - | 57516:21948-21967 | MS.gene061770:intergenic | 25.0% |
| !! | TTCATTTTACGAGAATACAT+TGG | + | 57516:21909-21928 | MS.gene061770:CDS | 25.0% |
| AGAGAAAGGATATTTAGTAG+GGG | - | 57516:22201-22220 | MS.gene061770:intergenic | 30.0% | |
| ATTTCGTTGTAGTCAATATC+AGG | - | 57516:21966-21985 | MS.gene061770:intergenic | 30.0% | |
| GAGAGAAAGGATATTTAGTA+GGG | - | 57516:22202-22221 | MS.gene061770:intergenic | 30.0% | |
| TATAAAAAGACTCAAGGAAG+AGG | + | 57516:22364-22383 | MS.gene061770:CDS | 30.0% | |
| TTTCGTTGTAGTCAATATCA+GGG | - | 57516:21965-21984 | MS.gene061770:intergenic | 30.0% | |
| ! | GTAGTGTTCATTAGTTTCAT+GGG | - | 57516:22420-22439 | MS.gene061770:intergenic | 30.0% |
| ! | TGTAGTGTTCATTAGTTTCA+TGG | - | 57516:22421-22440 | MS.gene061770:intergenic | 30.0% |
| !! | AATCGATTTCCATGTCATTT+TGG | + | 57516:21983-22002 | MS.gene061770:CDS | 30.0% |
| !! | ACTTTTGAAAAACTCCTAGT+AGG | + | 57516:22569-22588 | MS.gene061770:CDS | 30.0% |
| !!! | TCTTTTTCAATAGGAGAGAA+AGG | - | 57516:22215-22234 | MS.gene061770:intergenic | 30.0% |
| AAGATTTCTCCAACTACGTT+GGG | + | 57516:22336-22355 | MS.gene061770:CDS | 35.0% | |
| ACCACAGTGACGATATTAAT+TGG | + | 57516:22456-22475 | MS.gene061770:CDS | 35.0% | |
| ACTTACAACGAAGATGGAAA+AGG | + | 57516:22020-22039 | MS.gene061770:CDS | 35.0% | |
| ATCTTCGTTGTAAGTCTCAT+GGG | - | 57516:22015-22034 | MS.gene061770:intergenic | 35.0% | |
| CCACAGTGACGATATTAATT+GGG | + | 57516:22457-22476 | MS.gene061770:CDS | 35.0% | |
| CTCCTATTGAAAAAGAATCC+TGG | + | 57516:22219-22238 | MS.gene061770:CDS | 35.0% | |
| GAGAAAGGATATTTAGTAGG+GGG | - | 57516:22200-22219 | MS.gene061770:intergenic | 35.0% | |
| GGACGTTATAAAAAGACTCA+AGG | + | 57516:22358-22377 | MS.gene061770:CDS | 35.0% | |
| GGAGAGAAAGGATATTTAGT+AGG | - | 57516:22203-22222 | MS.gene061770:intergenic | 35.0% | |
| GTTGGAGAAATCTTCTTCAT+TGG | - | 57516:22330-22349 | MS.gene061770:intergenic | 35.0% | |
| ! | TGTTCATTAGTTTCATGGGA+TGG | - | 57516:22416-22435 | MS.gene061770:intergenic | 35.0% |
| !!! | CACCAGGATTCTTTTTCAAT+AGG | - | 57516:22224-22243 | MS.gene061770:intergenic | 35.0% |
| AAAAGACTCAAGGAAGAGGT+AGG | + | 57516:22368-22387 | MS.gene061770:CDS | 40.0% | |
| AACACTACAAGCTACTCTAC+AGG | + | 57516:22432-22451 | MS.gene061770:CDS | 40.0% | |
| ACACTACAAGCTACTCTACA+GGG | + | 57516:22433-22452 | MS.gene061770:CDS | 40.0% | |
| AGATTTCTCCAACTACGTTG+GGG | + | 57516:22337-22356 | MS.gene061770:CDS | 40.0% | |
| ATCCAAGACTACACGTTTGA+TGG | + | 57516:22275-22294 | MS.gene061770:CDS | 40.0% | |
| CAGATATCAAGATCCCAATC+GGG | - | 57516:22062-22081 | MS.gene061770:intergenic | 40.0% | |
| CATCTTCGTTGTAAGTCTCA+TGG | - | 57516:22016-22035 | MS.gene061770:intergenic | 40.0% | |
| CATGAGACTTACAACGAAGA+TGG | + | 57516:22014-22033 | MS.gene061770:CDS | 40.0% | |
| CCCAATTAATATCGTCACTG+TGG | - | 57516:22460-22479 | MS.gene061770:intergenic | 40.0% | |
| CGGAAAATTCTATCCCGATT+GGG | + | 57516:22046-22065 | MS.gene061770:CDS | 40.0% | |
| GAAGATTTCTCCAACTACGT+TGG | + | 57516:22335-22354 | MS.gene061770:CDS | 40.0% | |
| GAATCGATCAACAGTGTTAC+CGG | - | 57516:21892-21911 | MS.gene061770:intergenic | 40.0% | |
| GATCTATGTTAGACTCGCAA+GGG | + | 57516:22538-22557 | MS.gene061770:CDS | 40.0% | |
| GATTGGGATCTTGATATCTG+CGG | + | 57516:22062-22081 | MS.gene061770:CDS | 40.0% | |
| GCAGATATCAAGATCCCAAT+CGG | - | 57516:22063-22082 | MS.gene061770:intergenic | 40.0% | |
| TATCAAGCTCAAGTATCCCA+AGG | + | 57516:22103-22122 | MS.gene061770:CDS | 40.0% | |
| TCGTGATATACAAAGTGTGC+AGG | + | 57516:22151-22170 | MS.gene061770:CDS | 40.0% | |
| TGATCTATGTTAGACTCGCA+AGG | + | 57516:22537-22556 | MS.gene061770:CDS | 40.0% | |
| TTGCGAGTCTAACATAGATC+AGG | - | 57516:22538-22557 | MS.gene061770:intergenic | 40.0% | |
| ! | CCAATCGGGATAGAATTTTC+CGG | - | 57516:22048-22067 | MS.gene061770:intergenic | 40.0% |
| !! | GATCTTTTTGAGCGAATCGA+CGG | - | 57516:22255-22274 | MS.gene061770:intergenic | 40.0% |
| ACAAAGTCAGTCTCGTCAGT+GGG | - | 57516:22512-22531 | MS.gene061770:intergenic | 45.0% | |
| AGTTTCATGGGATGGAGCAA+TGG | - | 57516:22408-22427 | MS.gene061770:intergenic | 45.0% | |
| CCGGAAAATTCTATCCCGAT+TGG | + | 57516:22045-22064 | MS.gene061770:CDS | 45.0% | |
| CTAAGTACCACCTTGACCTT+GGG | - | 57516:22122-22141 | MS.gene061770:intergenic | 45.0% | |
| GCAGGATATCATCAAGCACA+AGG | + | 57516:22169-22188 | MS.gene061770:CDS | 45.0% | |
| GGAAGAGGTAGGTATCAATG+TGG | + | 57516:22379-22398 | MS.gene061770:CDS | 45.0% | |
| GGTTAGGCTGCATGTAGAAT+TGG | - | 57516:22490-22509 | MS.gene061770:intergenic | 45.0% | |
| GTCAAGGTGGTACTTAGCAT+TGG | + | 57516:22125-22144 | MS.gene061770:CDS | 45.0% | |
| GTTGTAAGTCTCATGGGCAA+AGG | - | 57516:22009-22028 | MS.gene061770:intergenic | 45.0% | |
| TACAAAGTCAGTCTCGTCAG+TGG | - | 57516:22513-22532 | MS.gene061770:intergenic | 45.0% | |
| TATAACGTCCCCAACGTAGT+TGG | - | 57516:22348-22367 | MS.gene061770:intergenic | 45.0% | |
| TGCCATCAAACGTGTAGTCT+TGG | - | 57516:22280-22299 | MS.gene061770:intergenic | 45.0% | |
| ! | AACGAAGATGGAAAAGGCAC+CGG | + | 57516:22026-22045 | MS.gene061770:CDS | 45.0% |
| ! | AAGGTGGTACTTAGCATTGG+TGG | + | 57516:22128-22147 | MS.gene061770:CDS | 45.0% |
| AGCTTGATATCCTGCACACG+TGG | - | 57516:22092-22111 | MS.gene061770:intergenic | 50.0% | |
| ATCGACGGCATTATCACACC+AGG | - | 57516:22240-22259 | MS.gene061770:intergenic | 50.0% | |
| CAAAGTCAGTCTCGTCAGTG+GGG | - | 57516:22511-22530 | MS.gene061770:intergenic | 50.0% | |
| CAAGTATCCCAAGGTCAAGG+TGG | + | 57516:22112-22131 | MS.gene061770:CDS | 50.0% | |
| GCTAAGTACCACCTTGACCT+TGG | - | 57516:22123-22142 | MS.gene061770:intergenic | 50.0% | |
| GCTCAAGTATCCCAAGGTCA+AGG | + | 57516:22109-22128 | MS.gene061770:CDS | 50.0% | |
| GGGCAAAGGCCAAAATGACA+TGG | - | 57516:21995-22014 | MS.gene061770:intergenic | 50.0% | |
| TCAGTCTCGTCAGTGGGGTT+AGG | - | 57516:22506-22525 | MS.gene061770:intergenic | 55.0% | |
| ! | GATCGGTGCTTGCTCCTACT+AGG | - | 57516:22586-22605 | MS.gene061770:intergenic | 55.0% |
| CTGCGGTATACCACGTGTGC+AGG | + | 57516:22079-22098 | MS.gene061770:CDS | 60.0% | |
| !! | GCAAGCACCGATCCACGTGA+CGG | + | 57516:22593-22612 | MS.gene061770:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57516 | gene | 21873 | 22617 | 21873 | ID=MS.gene061770 |
| 57516 | mRNA | 21873 | 22617 | 21873 | ID=MS.gene061770.t1;Parent=MS.gene061770 |
| 57516 | exon | 21873 | 22617 | 21873 | ID=MS.gene061770.t1.exon1;Parent=MS.gene061770.t1 |
| 57516 | CDS | 21873 | 22617 | 21873 | ID=cds.MS.gene061770.t1;Parent=MS.gene061770.t1 |
| Gene Sequence |
| Protein sequence |
