Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061771.t1 | RHN40044.1 | 68 | 253 | 58 | 8 | 1 | 248 | 1 | 235 | 1.40E-84 | 322.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061771.t1 | Q7M443 | 34.9 | 241 | 134 | 7 | 14 | 248 | 2 | 225 | 2.3e-27 | 124.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061771.t1 | A0A396GKD7 | 68.0 | 253 | 58 | 8 | 1 | 248 | 1 | 235 | 1.0e-84 | 322.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051860 | MS.gene061771 | 0.831517 | 1.60E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061771.t1 | MTR_0002s0040 | 56.757 | 259 | 83 | 7 | 1 | 248 | 1 | 241 | 5.50e-86 | 258 |
| MS.gene061771.t1 | MTR_0263s0020 | 56.757 | 259 | 83 | 7 | 1 | 248 | 1 | 241 | 4.12e-85 | 255 |
| MS.gene061771.t1 | MTR_1457s0010 | 66.310 | 187 | 42 | 6 | 65 | 248 | 1 | 169 | 2.84e-74 | 225 |
| MS.gene061771.t1 | MTR_0002s1060 | 52.326 | 258 | 91 | 10 | 4 | 248 | 6 | 244 | 5.06e-66 | 207 |
| MS.gene061771.t1 | MTR_0443s0040 | 40.856 | 257 | 111 | 10 | 14 | 247 | 16 | 254 | 7.23e-43 | 147 |
| MS.gene061771.t1 | MTR_0062s0090 | 40.726 | 248 | 114 | 8 | 14 | 244 | 4 | 235 | 3.54e-41 | 143 |
| MS.gene061771.t1 | MTR_0062s0040 | 40.726 | 248 | 114 | 8 | 14 | 244 | 4 | 235 | 3.54e-41 | 143 |
| MS.gene061771.t1 | MTR_0062s0060 | 40.726 | 248 | 114 | 8 | 14 | 244 | 4 | 235 | 3.54e-41 | 143 |
| MS.gene061771.t1 | MTR_0062s0020 | 39.200 | 250 | 120 | 6 | 14 | 247 | 4 | 237 | 3.20e-40 | 140 |
| MS.gene061771.t1 | MTR_6g079630 | 33.884 | 242 | 139 | 6 | 12 | 248 | 32 | 257 | 3.47e-31 | 117 |
| MS.gene061771.t1 | MTR_0062s0170 | 35.955 | 267 | 132 | 8 | 1 | 245 | 1 | 250 | 2.66e-30 | 115 |
| MS.gene061771.t1 | MTR_0062s0110 | 35.955 | 267 | 132 | 8 | 1 | 245 | 1 | 250 | 2.66e-30 | 115 |
| MS.gene061771.t1 | MTR_0062s0130 | 35.955 | 267 | 132 | 8 | 1 | 245 | 1 | 250 | 2.66e-30 | 115 |
| MS.gene061771.t1 | MTR_2g036240 | 35.955 | 267 | 132 | 8 | 1 | 245 | 1 | 250 | 2.66e-30 | 115 |
| MS.gene061771.t1 | MTR_2g036260 | 35.955 | 267 | 132 | 8 | 1 | 245 | 1 | 250 | 2.66e-30 | 115 |
| MS.gene061771.t1 | MTR_2g036290 | 35.955 | 267 | 132 | 8 | 1 | 245 | 1 | 250 | 2.66e-30 | 115 |
| MS.gene061771.t1 | MTR_8g046730 | 35.955 | 267 | 132 | 8 | 1 | 245 | 1 | 250 | 2.66e-30 | 115 |
| MS.gene061771.t1 | MTR_2g096030 | 34.167 | 240 | 137 | 7 | 14 | 248 | 31 | 254 | 8.10e-29 | 110 |
| MS.gene061771.t1 | MTR_2g096040 | 34.562 | 217 | 119 | 5 | 39 | 247 | 26 | 227 | 1.07e-26 | 104 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATATAAAAGATTTTGGGTA+TGG | - | 57526:1121-1140 | MS.gene061771:intergenic | 20.0% |
| !! | CAGGGAATATAAAAGATTTT+GGG | - | 57526:1126-1145 | MS.gene061771:intergenic | 25.0% |
| !! | TCAGGGAATATAAAAGATTT+TGG | - | 57526:1127-1146 | MS.gene061771:intergenic | 25.0% |
| !! | TTCATTTTACGAGAATACAT+TGG | + | 57526:1088-1107 | MS.gene061771:CDS | 25.0% |
| AGAGAAAGGATATTTAGTAG+GGG | - | 57526:1380-1399 | MS.gene061771:intergenic | 30.0% | |
| ATTTCGTTGTAGTCAATATC+AGG | - | 57526:1145-1164 | MS.gene061771:intergenic | 30.0% | |
| GAGAGAAAGGATATTTAGTA+GGG | - | 57526:1381-1400 | MS.gene061771:intergenic | 30.0% | |
| TATAAAAAGACTCAAGGAAG+AGG | + | 57526:1543-1562 | MS.gene061771:CDS | 30.0% | |
| TTTCGTTGTAGTCAATATCA+GGG | - | 57526:1144-1163 | MS.gene061771:intergenic | 30.0% | |
| ! | GTAGTGTTCATTAGTTTCAT+GGG | - | 57526:1599-1618 | MS.gene061771:intergenic | 30.0% |
| ! | TGTAGTGTTCATTAGTTTCA+TGG | - | 57526:1600-1619 | MS.gene061771:intergenic | 30.0% |
| !! | AATCGATTTCCATGTCATTT+TGG | + | 57526:1162-1181 | MS.gene061771:CDS | 30.0% |
| !! | ACTTTTGAAAAACTCCTAGT+AGG | + | 57526:1748-1767 | MS.gene061771:CDS | 30.0% |
| !!! | TCTTTTTCAATAGGAGAGAA+AGG | - | 57526:1394-1413 | MS.gene061771:intergenic | 30.0% |
| AAGATTTCTCCAACTACGTT+GGG | + | 57526:1515-1534 | MS.gene061771:CDS | 35.0% | |
| ACCACAGTGACGATATTAAT+TGG | + | 57526:1635-1654 | MS.gene061771:CDS | 35.0% | |
| ACTTACAACGAAGATGGAAA+AGG | + | 57526:1199-1218 | MS.gene061771:CDS | 35.0% | |
| ATCTTCGTTGTAAGTCTCAT+GGG | - | 57526:1194-1213 | MS.gene061771:intergenic | 35.0% | |
| CCACAGTGACGATATTAATT+GGG | + | 57526:1636-1655 | MS.gene061771:CDS | 35.0% | |
| CTCCTATTGAAAAAGAATCC+TGG | + | 57526:1398-1417 | MS.gene061771:CDS | 35.0% | |
| GAGAAAGGATATTTAGTAGG+GGG | - | 57526:1379-1398 | MS.gene061771:intergenic | 35.0% | |
| GGACGTTATAAAAAGACTCA+AGG | + | 57526:1537-1556 | MS.gene061771:CDS | 35.0% | |
| GGAGAGAAAGGATATTTAGT+AGG | - | 57526:1382-1401 | MS.gene061771:intergenic | 35.0% | |
| GTTGGAGAAATCTTCTTCAT+TGG | - | 57526:1509-1528 | MS.gene061771:intergenic | 35.0% | |
| ! | TGTTCATTAGTTTCATGGGA+TGG | - | 57526:1595-1614 | MS.gene061771:intergenic | 35.0% |
| !!! | CACCAGGATTCTTTTTCAAT+AGG | - | 57526:1403-1422 | MS.gene061771:intergenic | 35.0% |
| AAAAGACTCAAGGAAGAGGT+AGG | + | 57526:1547-1566 | MS.gene061771:CDS | 40.0% | |
| AACACTACAAGCTACTCTAC+AGG | + | 57526:1611-1630 | MS.gene061771:CDS | 40.0% | |
| ACACTACAAGCTACTCTACA+GGG | + | 57526:1612-1631 | MS.gene061771:CDS | 40.0% | |
| AGATTTCTCCAACTACGTTG+GGG | + | 57526:1516-1535 | MS.gene061771:CDS | 40.0% | |
| ATCCAAGACTACACGTTTGA+TGG | + | 57526:1454-1473 | MS.gene061771:CDS | 40.0% | |
| CAGATATCAAGATCCCAATC+GGG | - | 57526:1241-1260 | MS.gene061771:intergenic | 40.0% | |
| CATCTTCGTTGTAAGTCTCA+TGG | - | 57526:1195-1214 | MS.gene061771:intergenic | 40.0% | |
| CATGAGACTTACAACGAAGA+TGG | + | 57526:1193-1212 | MS.gene061771:CDS | 40.0% | |
| CCCAATTAATATCGTCACTG+TGG | - | 57526:1639-1658 | MS.gene061771:intergenic | 40.0% | |
| CGGAAAATTCTATCCCGATT+GGG | + | 57526:1225-1244 | MS.gene061771:CDS | 40.0% | |
| GAAGATTTCTCCAACTACGT+TGG | + | 57526:1514-1533 | MS.gene061771:CDS | 40.0% | |
| GAATCGATCAACAGTGTTAC+CGG | - | 57526:1071-1090 | MS.gene061771:intergenic | 40.0% | |
| GATCTATGTTAGACTCGCAA+GGG | + | 57526:1717-1736 | MS.gene061771:CDS | 40.0% | |
| GATTGGGATCTTGATATCTG+CGG | + | 57526:1241-1260 | MS.gene061771:CDS | 40.0% | |
| GCAGATATCAAGATCCCAAT+CGG | - | 57526:1242-1261 | MS.gene061771:intergenic | 40.0% | |
| TATCAAGCTCAAGTATCCCA+AGG | + | 57526:1282-1301 | MS.gene061771:CDS | 40.0% | |
| TCGTGATATACAAAGTGTGC+AGG | + | 57526:1330-1349 | MS.gene061771:CDS | 40.0% | |
| TGATCTATGTTAGACTCGCA+AGG | + | 57526:1716-1735 | MS.gene061771:CDS | 40.0% | |
| TTGCGAGTCTAACATAGATC+AGG | - | 57526:1717-1736 | MS.gene061771:intergenic | 40.0% | |
| ! | CCAATCGGGATAGAATTTTC+CGG | - | 57526:1227-1246 | MS.gene061771:intergenic | 40.0% |
| !! | GATCTTTTTGAGCGAATCGA+CGG | - | 57526:1434-1453 | MS.gene061771:intergenic | 40.0% |
| ACAAAGTCAGTCTCGTCAGT+GGG | - | 57526:1691-1710 | MS.gene061771:intergenic | 45.0% | |
| AGTTTCATGGGATGGAGCAA+TGG | - | 57526:1587-1606 | MS.gene061771:intergenic | 45.0% | |
| CCGGAAAATTCTATCCCGAT+TGG | + | 57526:1224-1243 | MS.gene061771:CDS | 45.0% | |
| CTAAGTACCACCTTGACCTT+GGG | - | 57526:1301-1320 | MS.gene061771:intergenic | 45.0% | |
| GCAGGATATCATCAAGCACA+AGG | + | 57526:1348-1367 | MS.gene061771:CDS | 45.0% | |
| GGAAGAGGTAGGTATCAATG+TGG | + | 57526:1558-1577 | MS.gene061771:CDS | 45.0% | |
| GGTTAGGCTGCATGTAGAAT+TGG | - | 57526:1669-1688 | MS.gene061771:intergenic | 45.0% | |
| GTCAAGGTGGTACTTAGCAT+TGG | + | 57526:1304-1323 | MS.gene061771:CDS | 45.0% | |
| GTTGTAAGTCTCATGGGCAA+AGG | - | 57526:1188-1207 | MS.gene061771:intergenic | 45.0% | |
| TACAAAGTCAGTCTCGTCAG+TGG | - | 57526:1692-1711 | MS.gene061771:intergenic | 45.0% | |
| TATAACGTCCCCAACGTAGT+TGG | - | 57526:1527-1546 | MS.gene061771:intergenic | 45.0% | |
| TGCCATCAAACGTGTAGTCT+TGG | - | 57526:1459-1478 | MS.gene061771:intergenic | 45.0% | |
| ! | AACGAAGATGGAAAAGGCAC+CGG | + | 57526:1205-1224 | MS.gene061771:CDS | 45.0% |
| ! | AAGGTGGTACTTAGCATTGG+TGG | + | 57526:1307-1326 | MS.gene061771:CDS | 45.0% |
| AGCTTGATATCCTGCACACG+TGG | - | 57526:1271-1290 | MS.gene061771:intergenic | 50.0% | |
| ATCGACGGCATTATCACACC+AGG | - | 57526:1419-1438 | MS.gene061771:intergenic | 50.0% | |
| CAAAGTCAGTCTCGTCAGTG+GGG | - | 57526:1690-1709 | MS.gene061771:intergenic | 50.0% | |
| CAAGTATCCCAAGGTCAAGG+TGG | + | 57526:1291-1310 | MS.gene061771:CDS | 50.0% | |
| GCTAAGTACCACCTTGACCT+TGG | - | 57526:1302-1321 | MS.gene061771:intergenic | 50.0% | |
| GCTCAAGTATCCCAAGGTCA+AGG | + | 57526:1288-1307 | MS.gene061771:CDS | 50.0% | |
| GGGCAAAGGCCAAAATGACA+TGG | - | 57526:1174-1193 | MS.gene061771:intergenic | 50.0% | |
| TCAGTCTCGTCAGTGGGGTT+AGG | - | 57526:1685-1704 | MS.gene061771:intergenic | 55.0% | |
| ! | GATCGGTGCTTGCTCCTACT+AGG | - | 57526:1765-1784 | MS.gene061771:intergenic | 55.0% |
| CTGCGGTATACCACGTGTGC+AGG | + | 57526:1258-1277 | MS.gene061771:CDS | 60.0% | |
| !! | GCAAGCACCGATCCACGTGA+CGG | + | 57526:1772-1791 | MS.gene061771:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57526 | gene | 1052 | 1796 | 1052 | ID=MS.gene061771 |
| 57526 | mRNA | 1052 | 1796 | 1052 | ID=MS.gene061771.t1;Parent=MS.gene061771 |
| 57526 | exon | 1052 | 1796 | 1052 | ID=MS.gene061771.t1.exon1;Parent=MS.gene061771.t1 |
| 57526 | CDS | 1052 | 1796 | 1052 | ID=cds.MS.gene061771.t1;Parent=MS.gene061771.t1 |
| Gene Sequence |
| Protein sequence |
