AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061875

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061875.t1 MTR_7g099580 76.174 298 35 3 1 284 9 284 2.20e-154 439
MS.gene061875.t1 MTR_1g069110 47.287 387 172 8 1 362 15 394 4.48e-111 333
MS.gene061875.t1 MTR_7g099570 73.333 195 51 1 1 195 6 199 1.40e-91 287
MS.gene061875.t1 MTR_7g099570 73.750 160 42 0 211 370 398 557 2.36e-79 256
MS.gene061875.t1 MTR_8g072510 26.371 383 223 9 2 333 8 382 3.98e-34 132
MS.gene061875.t1 MTR_8g069880 26.214 412 244 10 7 375 10 404 4.98e-34 132
MS.gene061875.t1 MTR_8g068990 26.374 364 206 6 7 327 15 359 1.68e-32 127
MS.gene061875.t1 MTR_8g073260 27.378 347 201 9 7 311 14 351 1.88e-32 127
MS.gene061875.t1 MTR_7g099550 55.963 109 43 2 63 171 136 239 6.62e-31 118
MS.gene061875.t1 MTR_3g016210 25.113 442 248 12 3 383 9 428 6.65e-31 122
MS.gene061875.t1 MTR_8g039620 25.538 372 223 9 4 329 14 377 6.39e-30 120
MS.gene061875.t1 MTR_7g099600 86.154 65 9 0 306 370 1 65 2.45e-29 109
MS.gene061875.t1 MTR_8g062410 27.041 392 242 10 3 360 9 390 8.06e-29 117
MS.gene061875.t1 MTR_7g021090 25.635 394 212 13 2 341 7 373 2.63e-28 115
MS.gene061875.t1 MTR_8g467780 25.428 409 241 9 3 360 10 405 1.36e-27 114
MS.gene061875.t1 MTR_8g066670 24.533 375 229 10 1 330 1072 1437 2.97e-25 108
MS.gene061875.t1 MTR_1g059930 25.484 310 184 6 4 273 12 314 4.35e-25 106
MS.gene061875.t1 MTR_7g451420 25.962 312 188 7 1 274 6 312 9.16e-22 95.1
MS.gene061875.t1 MTR_3g105650 30.366 191 120 3 7 189 8 193 1.47e-18 87.4
MS.gene061875.t1 MTR_8g040930 22.051 390 227 11 46 375 1 373 3.07e-18 85.9
MS.gene061875.t1 MTR_8g041240 22.051 390 227 11 46 375 1 373 3.07e-18 85.9
MS.gene061875.t1 MTR_7g087540 39.669 121 68 3 232 349 24 142 4.02e-18 82.0
MS.gene061875.t1 MTR_8g019260 36.207 116 74 0 244 359 15 130 7.37e-17 78.2
MS.gene061875.t1 MTR_8g019110 36.207 116 74 0 244 359 15 130 7.37e-17 78.2
MS.gene061875.t1 MTR_8g019290 30.065 153 89 1 216 368 5 139 1.06e-16 77.8
MS.gene061875.t1 MTR_1g115575 23.150 419 224 14 4 357 135 520 2.48e-16 81.3
MS.gene061875.t1 MTR_4g062100 24.862 362 214 13 30 357 133 470 3.31e-16 80.5
MS.gene061875.t1 MTR_7g100390 25.061 411 236 14 4 357 53 448 1.16e-15 79.0
MS.gene061875.t1 MTR_2g101310 32.298 161 102 3 35 195 137 290 2.22e-15 78.2
MS.gene061875.t1 MTR_7g117620 32.692 156 94 4 39 193 119 264 3.38e-15 77.4
MS.gene061875.t1 MTR_7g117620 24.734 376 236 12 7 357 123 476 1.12e-13 72.8
MS.gene061875.t1 MTR_7g117620 27.273 198 104 6 28 190 35 227 4.26e-11 64.7
MS.gene061875.t1 MTR_2g101320 31.098 164 106 2 32 195 134 290 6.10e-15 77.0
MS.gene061875.t1 MTR_2g101310 32.298 161 102 3 35 195 137 290 9.07e-15 76.3
MS.gene061875.t1 MTR_5g085480 31.183 186 118 5 4 188 54 230 1.45e-14 75.5
MS.gene061875.t1 MTR_5g085480 31.183 186 118 5 4 188 214 390 2.10e-14 75.5
MS.gene061875.t1 MTR_7g099560 30.058 173 60 5 212 382 50 163 2.30e-14 72.8
MS.gene061875.t1 MTR_2g096190 27.979 193 127 5 4 195 70 251 2.49e-14 74.7
MS.gene061875.t1 MTR_2g096190 25.000 408 235 18 7 378 108 480 4.90e-13 70.9
MS.gene061875.t1 MTR_0470s0010 32.558 129 78 3 241 362 15 141 2.77e-14 71.2
MS.gene061875.t1 MTR_8g019020 32.558 129 78 3 241 362 15 141 2.77e-14 71.2
MS.gene061875.t1 MTR_5g085500 39.167 120 65 5 73 190 8 121 6.78e-13 70.1
MS.gene061875.t1 MTR_7g100430 30.380 158 99 3 39 195 161 308 2.17e-12 68.9
MS.gene061875.t1 MTR_7g055630 32.237 152 86 3 244 378 16 167 5.62e-12 64.7
MS.gene061875.t1 MTR_8g019280 33.945 109 72 0 260 368 1 109 6.47e-12 63.5
MS.gene061875.t1 MTR_8g019450 30.519 154 74 4 239 360 32 184 7.78e-12 65.5
MS.gene061875.t1 MTR_7g087560 31.967 122 60 2 232 353 24 122 4.31e-11 61.6
MS.gene061875.t1 MTR_0514s0010 26.923 182 92 6 210 359 13 185 4.65e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061875.t1 AT5G15500 26.154 390 210 11 2 337 4 369 1.09e-26 111
MS.gene061875.t1 AT4G10720 25.519 337 191 7 11 301 12 334 1.20e-25 107
MS.gene061875.t1 AT1G14480 28.231 294 181 10 3 281 4 282 3.80e-25 106
MS.gene061875.t1 AT4G10720 25.962 312 180 6 11 276 12 318 5.28e-25 106
MS.gene061875.t1 AT1G14480 23.853 327 196 6 3 281 4 325 4.25e-23 100
MS.gene061875.t1 AT5G51160 23.404 329 230 5 26 332 34 362 1.96e-21 95.9
MS.gene061875.t1 AT1G14500 24.224 322 190 5 3 276 4 319 2.31e-21 95.5
MS.gene061875.t1 AT5G54610 33.514 185 112 4 10 188 11 190 1.04e-20 93.6
MS.gene061875.t1 AT5G54620 23.718 312 188 6 2 268 3 309 1.87e-20 92.8
MS.gene061875.t1 AT3G13950 38.406 138 78 4 216 349 6 140 1.11e-19 87.0
MS.gene061875.t1 AT5G60070 30.645 186 108 7 39 217 111 282 3.78e-17 83.6
MS.gene061875.t1 AT2G31820 32.474 194 117 6 4 195 193 374 1.78e-16 81.6
MS.gene061875.t1 AT2G31820 23.478 345 219 11 38 357 260 584 2.77e-11 65.9
MS.gene061875.t1 AT1G10340 24.000 425 201 13 3 359 135 505 1.98e-15 78.6
MS.gene061875.t1 AT4G13266 33.824 136 80 5 234 362 24 156 2.03e-15 75.5
MS.gene061875.t1 AT1G34050 24.607 382 216 13 25 359 140 496 1.03e-14 76.3
MS.gene061875.t1 AT5G50140 30.952 168 96 4 35 188 26 187 3.85e-14 74.3
MS.gene061875.t1 AT3G12360 24.703 421 215 16 4 357 121 506 4.29e-14 74.3
MS.gene061875.t1 AT3G12360 24.703 421 215 16 4 357 133 518 4.44e-14 74.3
MS.gene061875.t1 AT1G05640 30.570 193 122 6 4 195 157 338 6.45e-14 73.9
MS.gene061875.t1 AT1G05640 25.439 228 121 7 37 220 114 336 7.49e-13 70.5
MS.gene061875.t1 AT1G07710 27.919 197 128 5 4 197 70 255 4.77e-13 70.9
MS.gene061875.t1 AT1G07710 27.919 197 128 5 4 197 70 255 4.77e-13 70.9
MS.gene061875.t1 AT3G09550 31.013 158 96 5 40 194 182 329 5.96e-12 67.8
MS.gene061875.t1 AT3G09550 31.013 158 96 5 40 194 182 329 5.96e-12 67.8
MS.gene061875.t1 AT3G09550 31.013 158 96 5 40 194 189 336 6.45e-12 67.8
MS.gene061875.t1 AT4G03500 23.164 354 213 11 4 327 196 520 8.78e-12 67.4
MS.gene061875.t1 AT4G03500 23.164 354 213 11 4 327 196 520 8.78e-12 67.4
MS.gene061875.t1 AT4G03500 23.164 354 213 11 4 327 219 543 8.96e-12 67.4

Find 74 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTTAATGATTTCGGTTTC+TGG 0.183664 7.4:+10651929 None:intergenic
GTTCACTTCAGCTGCTAAAT+TGG 0.191406 7.4:+10652132 None:intergenic
TCCAACTACCTTGGTTTATT+AGG 0.226783 7.4:+10650676 None:intergenic
TGGATTTCCACTTGGGAATA+AGG 0.247146 7.4:-10650394 MS.gene061875:CDS
CTCTACAATGCATCATTAAA+TGG 0.285327 7.4:-10652301 MS.gene061875:CDS
AAATTACAAGAGTGGTGTTT+TGG 0.298168 7.4:-10650197 MS.gene061875:CDS
AAACATCACCTTAATGATTT+CGG 0.302136 7.4:+10651922 None:intergenic
TCGCAAACTGGGATATGTTT+TGG 0.305620 7.4:-10650262 MS.gene061875:CDS
TCTTTGGATCAATGTTCTTA+AGG 0.306596 7.4:+10652267 None:intergenic
AGGACGTGTGGAAGCAATTA+AGG 0.310242 7.4:-10651969 MS.gene061875:CDS
TGTGTTGACTGTGAATTCTT+TGG 0.332031 7.4:+10650771 None:intergenic
TCTTTCTTACTTCTAGTTGT+TGG 0.336507 7.4:-10650228 MS.gene061875:CDS
TCAAAATGAGAGGGTCTCTT+TGG 0.353711 7.4:+10652251 None:intergenic
TGCTTCTCTGTGTGTGGTGT+TGG 0.358882 7.4:-10650427 MS.gene061875:CDS
GCCAGAAACCGAAATCATTA+AGG 0.359546 7.4:-10651930 MS.gene061875:CDS
AGGACGTTGCCCTCTTCATT+TGG 0.363159 7.4:-10652104 MS.gene061875:CDS
CTTCATTTGGCTTCTGCAAA+AGG 0.368217 7.4:-10652091 MS.gene061875:CDS
CTGCAGTTGTGGGTTATGTT+TGG 0.382850 7.4:-10650497 MS.gene061875:CDS
GCAGCTGAAGTGAACTTAGA+AGG 0.388437 7.4:-10652124 MS.gene061875:CDS
AAAGACAAAAGAACAATTCA+TGG 0.391103 7.4:-10650646 MS.gene061875:CDS
TGTCAGACGTGCACAAATAA+AGG 0.392121 7.4:-10651747 MS.gene061875:intron
AATATAAATTTCATAAAATC+TGG 0.393458 7.4:+10650471 None:intergenic
TTCACTTCAGCTGCTAAATT+GGG 0.395520 7.4:+10652133 None:intergenic
CTCACATACATGTGGGCACT+TGG 0.410282 7.4:-10650318 MS.gene061875:CDS
TAAGTCCACCAGGCAGTGTT+TGG 0.416873 7.4:-10650581 MS.gene061875:CDS
CTCATCAGTGGATTTCCACT+TGG 0.422915 7.4:-10650402 MS.gene061875:CDS
TTATTACAGAATTCGCAAAC+TGG 0.438524 7.4:-10650274 MS.gene061875:intron
TTGCACATAGCCTGTTTGCT+TGG 0.442386 7.4:-10652193 MS.gene061875:CDS
CTCGAAGTTTCCAAGCAAAC+AGG 0.446295 7.4:+10652183 None:intergenic
TGAAGCAGGCACTGCAGTTG+TGG 0.460591 7.4:-10650508 MS.gene061875:CDS
TCTTCTTGCCAAACACTGCC+TGG 0.477194 7.4:+10650573 None:intergenic
ACCTAATAAACCAAGGTAGT+TGG 0.477504 7.4:-10650677 MS.gene061875:CDS
TTCATTTGGCTTCTGCAAAA+GGG 0.485852 7.4:-10652090 MS.gene061875:CDS
ACTTATGATTTCTGTGAAGC+AGG 0.486383 7.4:-10650522 MS.gene061875:CDS
TTCTTTGGTGATGTTTGTGT+TGG 0.490831 7.4:+10650786 None:intergenic
GGTGATGTTTGTGTTGGTAG+TGG 0.503491 7.4:+10650792 None:intergenic
TGCAATGGTGTTTCAGAGAA+GGG 0.506363 7.4:+10652211 None:intergenic
ACTTGGGAATAAGGCCATGA+TGG 0.509567 7.4:-10650385 MS.gene061875:CDS
CTTGGGAATAAGGCCATGAT+GGG 0.510342 7.4:-10650384 MS.gene061875:CDS
GCGAACACACAAGTGTAGAA+TGG 0.520677 7.4:+10651880 None:intergenic
TCATCAGTGGATTTCCACTT+GGG 0.523634 7.4:-10650401 MS.gene061875:CDS
TTTCTTTGCTTCTCTGTGTG+TGG 0.530696 7.4:-10650433 MS.gene061875:CDS
AACTGCTAAAATCCCCATCA+TGG 0.551879 7.4:+10650371 None:intergenic
ATACATGTGGGCACTTGGAT+TGG 0.556164 7.4:-10650313 MS.gene061875:intron
TATTACAGAATTCGCAAACT+GGG 0.559117 7.4:-10650273 MS.gene061875:intron
GTGGTGTTGGTTCTCATCAG+TGG 0.560935 7.4:-10650414 MS.gene061875:CDS
GTGCAATGGTGTTTCAGAGA+AGG 0.565252 7.4:+10652210 None:intergenic
GAAGCAGGCACTGCAGTTGT+GGG 0.565583 7.4:-10650507 MS.gene061875:CDS
AATCATAAGTAGTGCATGCA+TGG 0.567093 7.4:+10650535 None:intergenic
TTTGATTCAAATTACAAGAG+TGG 0.567229 7.4:-10650205 MS.gene061875:CDS
TTGCAGAAGCCAAATGAAGA+GGG 0.567237 7.4:+10652095 None:intergenic
AACAACACAGACGCAACAGA+TGG 0.576839 7.4:-10650728 MS.gene061875:CDS
GTCCTCTCATTGCAGCAAAG+TGG 0.579978 7.4:+10651987 None:intergenic
TTATATGTCAAAGACAAAGA+AGG 0.586486 7.4:-10651791 MS.gene061875:CDS
CAATCAGTAATAAGTCCACC+AGG 0.590985 7.4:-10650591 MS.gene061875:CDS
ATCCACTTTGCTGCAATGAG+AGG 0.593694 7.4:-10651989 MS.gene061875:CDS
TTCCTCTCACTAGCTGCACG+AGG 0.594845 7.4:+10651831 None:intergenic
AGCAAACAGGCTATGTGCAA+TGG 0.598670 7.4:+10652196 None:intergenic
CTCATTGCAGCAAAGTGGAT+AGG 0.598957 7.4:+10651992 None:intergenic
ATGTTTGAGACTGATCATCA+TGG 0.605579 7.4:-10651905 MS.gene061875:CDS
TCATGTTGCTCACATACATG+TGG 0.606238 7.4:-10650326 MS.gene061875:CDS
TTTGCAGAAGCCAAATGAAG+AGG 0.607614 7.4:+10652094 None:intergenic
GAAACTTTGCTCAAAATGAG+AGG 0.611802 7.4:+10652241 None:intergenic
AAACTTTGCTCAAAATGAGA+GGG 0.617414 7.4:+10652242 None:intergenic
ATCCTCGTGCAGCTAGTGAG+AGG 0.624423 7.4:-10651833 MS.gene061875:CDS
TCTTGCCAAACACTGCCTGG+TGG 0.647998 7.4:+10650576 None:intergenic
CATGTTGCTCACATACATGT+GGG 0.656114 7.4:-10650325 MS.gene061875:CDS
TGTTCGCTATAACCATCTAG+AGG 0.665347 7.4:-10651864 MS.gene061875:CDS
ATCATGGCCTTATTCCCAAG+TGG 0.672138 7.4:+10650387 None:intergenic
AACTAGAAGTAAGAAAGACC+AGG 0.672399 7.4:+10650233 None:intergenic
TGCTGCAATGAGAGGACGTG+TGG 0.676824 7.4:-10651981 MS.gene061875:CDS
TTGGGAATAAGGCCATGATG+GGG 0.695312 7.4:-10650383 MS.gene061875:CDS
ACGACATACCTAATAAACCA+AGG 0.719709 7.4:-10650684 MS.gene061875:CDS
TGGCAAGAAGATACAAAACA+CGG 0.732277 7.4:-10650561 MS.gene061875:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATAAATTTCATAAAATC+TGG + chr7.4:10652034-10652053 None:intergenic 10.0%
!! ATAAATAATTTGAACAACAT+GGG - chr7.4:10651464-10651483 MS.gene061875:intron 15.0%
!! GTGATTTGATATAAATAATT+TGG + chr7.4:10650838-10650857 None:intergenic 15.0%
!! TATAAATAATTTGAACAACA+TGG - chr7.4:10651463-10651482 MS.gene061875:intron 15.0%
!! TTTATAGACTTGAAAATTTA+AGG - chr7.4:10651150-10651169 MS.gene061875:intron 15.0%
!!! CTATTTTAATCTATAACAAA+CGG - chr7.4:10650860-10650879 MS.gene061875:intron 15.0%
!!! TTCAAGTTATTTTAGAAAAA+TGG + chr7.4:10650790-10650809 None:intergenic 15.0%
!!! TTTGTTATAGATTAAAATAG+AGG + chr7.4:10650860-10650879 None:intergenic 15.0%
!! ATATAAACCATTACTGTTAT+TGG - chr7.4:10651048-10651067 MS.gene061875:intron 20.0%
!! ATTCTGTAATAAACATGATT+AGG + chr7.4:10652220-10652239 None:intergenic 20.0%
!! TATAAACCATTACTGTTATT+GGG - chr7.4:10651049-10651068 MS.gene061875:intron 20.0%
!! TCAACATAAAACAATAGAAA+AGG - chr7.4:10651373-10651392 MS.gene061875:intron 20.0%
!! TGGTTTATATACAAGAATTA+AGG + chr7.4:10651038-10651057 None:intergenic 20.0%
! AAACATCACCTTAATGATTT+CGG + chr7.4:10650583-10650602 None:intergenic 25.0%
! AAAGACAAAAGAACAATTCA+TGG - chr7.4:10651856-10651875 MS.gene061875:CDS 25.0%
! ATGAACATGTAAGTTTCATA+CGG + chr7.4:10651354-10651373 None:intergenic 25.0%
! TTATATGTCAAAGACAAAGA+AGG - chr7.4:10650711-10650730 MS.gene061875:CDS 25.0%
! TTTGATTCAAATTACAAGAG+TGG - chr7.4:10652297-10652316 MS.gene061875:CDS 25.0%
!! TATGAATGTGTTCTTTTAGA+CGG + chr7.4:10651243-10651262 None:intergenic 25.0%
!! TATTTTGTGACATTGTCATT+TGG + chr7.4:10651646-10651665 None:intergenic 25.0%
AAACTTTGCTCAAAATGAGA+GGG + chr7.4:10650263-10650282 None:intergenic 30.0%
CAATCAATACTCTTCACATT+GGG + chr7.4:10651105-10651124 None:intergenic 30.0%
CTCTACAATGCATCATTAAA+TGG - chr7.4:10650201-10650220 MS.gene061875:CDS 30.0%
GATCATATGTTAGCTAAAGA+AGG - chr7.4:10651551-10651570 MS.gene061875:intron 30.0%
TATTACAGAATTCGCAAACT+GGG - chr7.4:10652229-10652248 MS.gene061875:CDS 30.0%
TCAATCAATACTCTTCACAT+TGG + chr7.4:10651106-10651125 None:intergenic 30.0%
TCTTTGGATCAATGTTCTTA+AGG + chr7.4:10650238-10650257 None:intergenic 30.0%
TGAACATGTAAGTTTCATAC+GGG + chr7.4:10651353-10651372 None:intergenic 30.0%
TTATTACAGAATTCGCAAAC+TGG - chr7.4:10652228-10652247 MS.gene061875:CDS 30.0%
! AAATTACAAGAGTGGTGTTT+TGG - chr7.4:10652305-10652324 MS.gene061875:CDS 30.0%
! ATCCAAAAACTTGCTTCATT+AGG - chr7.4:10650469-10650488 MS.gene061875:CDS 30.0%
! GTACATTTGAAGTGATATTG+CGG + chr7.4:10651192-10651211 None:intergenic 30.0%
! TCCAAAAACTTGCTTCATTA+GGG - chr7.4:10650470-10650489 MS.gene061875:CDS 30.0%
! TCTATAAAGTCTAGTCGTTT+TGG + chr7.4:10651138-10651157 None:intergenic 30.0%
! TCTTTCTTACTTCTAGTTGT+TGG - chr7.4:10652274-10652293 MS.gene061875:CDS 30.0%
! TTTTATCAATCCAACTACCT+TGG + chr7.4:10651838-10651857 None:intergenic 30.0%
!! ATTTTCGGAGCATTTTTACT+TGG + chr7.4:10651300-10651319 None:intergenic 30.0%
AACTAGAAGTAAGAAAGACC+AGG + chr7.4:10652272-10652291 None:intergenic 35.0%
AATCATAAGTAGTGCATGCA+TGG + chr7.4:10651970-10651989 None:intergenic 35.0%
ACCTAATAAACCAAGGTAGT+TGG - chr7.4:10651825-10651844 MS.gene061875:CDS 35.0%
ACCTTAATGATTTCGGTTTC+TGG + chr7.4:10650576-10650595 None:intergenic 35.0%
ACGACATACCTAATAAACCA+AGG - chr7.4:10651818-10651837 MS.gene061875:CDS 35.0%
ACTTATGATTTCTGTGAAGC+AGG - chr7.4:10651980-10651999 MS.gene061875:CDS 35.0%
GAAACTTTGCTCAAAATGAG+AGG + chr7.4:10650264-10650283 None:intergenic 35.0%
GGATAAATTAAAGAGAGCGA+AGG + chr7.4:10651332-10651351 None:intergenic 35.0%
TATGTTAACTTCAGTTGACG+AGG + chr7.4:10651621-10651640 None:intergenic 35.0%
TCCAACTACCTTGGTTTATT+AGG + chr7.4:10651829-10651848 None:intergenic 35.0%
TGAGAACCCAATAACAGTAA+TGG + chr7.4:10651058-10651077 None:intergenic 35.0%
TGGCAAGAAGATACAAAACA+CGG - chr7.4:10651941-10651960 MS.gene061875:CDS 35.0%
TTAAGGAAAAAGCAGACTTG+AGG + chr7.4:10651021-10651040 None:intergenic 35.0%
TTCACTTCAGCTGCTAAATT+GGG + chr7.4:10650372-10650391 None:intergenic 35.0%
TTTAACACCATTGGATATGC+TGG - chr7.4:10651487-10651506 MS.gene061875:intron 35.0%
! ATGTTTGAGACTGATCATCA+TGG - chr7.4:10650597-10650616 MS.gene061875:CDS 35.0%
! TCCCTAATGAAGCAAGTTTT+TGG + chr7.4:10650474-10650493 None:intergenic 35.0%
! TTCATTTGGCTTCTGCAAAA+GGG - chr7.4:10650412-10650431 MS.gene061875:CDS 35.0%
!! CGAAGGTGTACATACATTTT+CGG + chr7.4:10651315-10651334 None:intergenic 35.0%
!! TGTGTTGACTGTGAATTCTT+TGG + chr7.4:10651734-10651753 None:intergenic 35.0%
!! TTCTTTGGTGATGTTTGTGT+TGG + chr7.4:10651719-10651738 None:intergenic 35.0%
!! TTGGAAACTTCGAGTTTTGT+CGG - chr7.4:10650328-10650347 MS.gene061875:CDS 35.0%
!!! AAACTGGGATATGTTTTGGT+TGG - chr7.4:10652244-10652263 MS.gene061875:CDS 35.0%
AAAGGCTCAACTTGAACACA+AGG + chr7.4:10650994-10651013 None:intergenic 40.0%
AACTGCTAAAATCCCCATCA+TGG + chr7.4:10652134-10652153 None:intergenic 40.0%
ACACAAGGTCAGATAGAATC+AGG + chr7.4:10650979-10650998 None:intergenic 40.0%
AGAATCAGGGTGAGCTAAAA+TGG + chr7.4:10650965-10650984 None:intergenic 40.0%
AGCTAAAGAAGGAGCTCAAA+GGG - chr7.4:10651562-10651581 MS.gene061875:intron 40.0%
CAACATGGGTTTAACACCAT+TGG - chr7.4:10651478-10651497 MS.gene061875:intron 40.0%
CAATCAGTAATAAGTCCACC+AGG - chr7.4:10651911-10651930 MS.gene061875:CDS 40.0%
CACAAGGTCAGATAGAATCA+GGG + chr7.4:10650978-10650997 None:intergenic 40.0%
CATGTTGCTCACATACATGT+GGG - chr7.4:10652177-10652196 MS.gene061875:CDS 40.0%
GCCAGAAACCGAAATCATTA+AGG - chr7.4:10650572-10650591 MS.gene061875:CDS 40.0%
GTTCACTTCAGCTGCTAAAT+TGG + chr7.4:10650373-10650392 None:intergenic 40.0%
TAGCTAAAGAAGGAGCTCAA+AGG - chr7.4:10651561-10651580 MS.gene061875:intron 40.0%
TCAAAATGAGAGGGTCTCTT+TGG + chr7.4:10650254-10650273 None:intergenic 40.0%
TCATGTTGCTCACATACATG+TGG - chr7.4:10652176-10652195 MS.gene061875:CDS 40.0%
TGCAATGGTGTTTCAGAGAA+GGG + chr7.4:10650294-10650313 None:intergenic 40.0%
TGGATTTCCACTTGGGAATA+AGG - chr7.4:10652108-10652127 MS.gene061875:CDS 40.0%
TGTCAGACGTGCACAAATAA+AGG - chr7.4:10650755-10650774 MS.gene061875:CDS 40.0%
TGTTATTGGGTTCTCATAGC+AGG - chr7.4:10651062-10651081 MS.gene061875:intron 40.0%
TGTTCGCTATAACCATCTAG+AGG - chr7.4:10650638-10650657 MS.gene061875:CDS 40.0%
TTGCAGAAGCCAAATGAAGA+GGG + chr7.4:10650410-10650429 None:intergenic 40.0%
TTTCTTTGCTTCTCTGTGTG+TGG - chr7.4:10652069-10652088 MS.gene061875:CDS 40.0%
TTTGCAGAAGCCAAATGAAG+AGG + chr7.4:10650411-10650430 None:intergenic 40.0%
! CTTCATTTGGCTTCTGCAAA+AGG - chr7.4:10650411-10650430 MS.gene061875:CDS 40.0%
! GGATTTTCAAAGCCTCTAGA+TGG + chr7.4:10650653-10650672 None:intergenic 40.0%
! TCATCAGTGGATTTCCACTT+GGG - chr7.4:10652101-10652120 MS.gene061875:CDS 40.0%
! TCGCAAACTGGGATATGTTT+TGG - chr7.4:10652240-10652259 MS.gene061875:CDS 40.0%
!!! GATATGTTTTGGTTGGTACC+TGG - chr7.4:10652251-10652270 MS.gene061875:CDS 40.0%
AACAACACAGACGCAACAGA+TGG - chr7.4:10651774-10651793 MS.gene061875:CDS 45.0%
AACTCACCTAGCAAGACAGT+TGG + chr7.4:10650926-10650945 None:intergenic 45.0%
ACTTGGGAATAAGGCCATGA+TGG - chr7.4:10652117-10652136 MS.gene061875:CDS 45.0%
AGCAAACAGGCTATGTGCAA+TGG + chr7.4:10650309-10650328 None:intergenic 45.0%
AGCTAAAATGGTATGCAGGC+AGG + chr7.4:10650953-10650972 None:intergenic 45.0%
AGGACGTGTGGAAGCAATTA+AGG - chr7.4:10650533-10650552 MS.gene061875:CDS 45.0%
ATACATGTGGGCACTTGGAT+TGG - chr7.4:10652189-10652208 MS.gene061875:CDS 45.0%
ATCCACTTTGCTGCAATGAG+AGG - chr7.4:10650513-10650532 MS.gene061875:CDS 45.0%
CTAGCAAGACAGTTGGAGTT+GGG + chr7.4:10650919-10650938 None:intergenic 45.0%
CTCATTGCAGCAAAGTGGAT+AGG + chr7.4:10650513-10650532 None:intergenic 45.0%
CTCGAAGTTTCCAAGCAAAC+AGG + chr7.4:10650322-10650341 None:intergenic 45.0%
CTGTGATCCAGCATATCCAA+TGG + chr7.4:10651497-10651516 None:intergenic 45.0%
CTTGGGAATAAGGCCATGAT+GGG - chr7.4:10652118-10652137 MS.gene061875:CDS 45.0%
GCAGCTGAAGTGAACTTAGA+AGG - chr7.4:10650378-10650397 MS.gene061875:CDS 45.0%
GCGAACACACAAGTGTAGAA+TGG + chr7.4:10650625-10650644 None:intergenic 45.0%
GGTGAGCTAAAATGGTATGC+AGG + chr7.4:10650957-10650976 None:intergenic 45.0%
GTGCAATGGTGTTTCAGAGA+AGG + chr7.4:10650295-10650314 None:intergenic 45.0%
TTGCACATAGCCTGTTTGCT+TGG - chr7.4:10650309-10650328 MS.gene061875:intron 45.0%
TTGGGAATAAGGCCATGATG+GGG - chr7.4:10652119-10652138 MS.gene061875:CDS 45.0%
! CTCATCAGTGGATTTCCACT+TGG - chr7.4:10652100-10652119 MS.gene061875:CDS 45.0%
! CTGCAGTTGTGGGTTATGTT+TGG - chr7.4:10652005-10652024 MS.gene061875:CDS 45.0%
! GGTGATGTTTGTGTTGGTAG+TGG + chr7.4:10651713-10651732 None:intergenic 45.0%
!! ATCATGGCCTTATTCCCAAG+TGG + chr7.4:10652118-10652137 None:intergenic 45.0%
!! GTGTTCAAGTTGAGCCTTTG+AGG - chr7.4:10650995-10651014 MS.gene061875:intron 45.0%
AAGCAGACTTGAGGCCTCAA+AGG + chr7.4:10651012-10651031 None:intergenic 50.0%
ACTTCAGTTGACGAGGAAGC+TGG + chr7.4:10651614-10651633 None:intergenic 50.0%
AGGACGTTGCCCTCTTCATT+TGG - chr7.4:10650398-10650417 MS.gene061875:CDS 50.0%
CCAACTCCAACTGTCTTGCT+AGG - chr7.4:10650917-10650936 MS.gene061875:intron 50.0%
CCTAGCAAGACAGTTGGAGT+TGG + chr7.4:10650920-10650939 None:intergenic 50.0%
CTCACATACATGTGGGCACT+TGG - chr7.4:10652184-10652203 MS.gene061875:CDS 50.0%
GAAGTGATATTGCGGTGCGT+GGG + chr7.4:10651184-10651203 None:intergenic 50.0%
GTCCTCTCATTGCAGCAAAG+TGG + chr7.4:10650518-10650537 None:intergenic 50.0%
TAAGTCCACCAGGCAGTGTT+TGG - chr7.4:10651921-10651940 MS.gene061875:CDS 50.0%
TCTTCTTGCCAAACACTGCC+TGG + chr7.4:10651932-10651951 None:intergenic 50.0%
TGAAGTGATATTGCGGTGCG+TGG + chr7.4:10651185-10651204 None:intergenic 50.0%
TGCTTCTCTGTGTGTGGTGT+TGG - chr7.4:10652075-10652094 MS.gene061875:CDS 50.0%
!! GTGGTGTTGGTTCTCATCAG+TGG - chr7.4:10652088-10652107 MS.gene061875:CDS 50.0%
!! GTTTGTGTTGGTAGTGGCTG+TGG + chr7.4:10651707-10651726 None:intergenic 50.0%
ATCCTCGTGCAGCTAGTGAG+AGG - chr7.4:10650669-10650688 MS.gene061875:CDS 55.0%
GCAAGACAGTTGGAGTTGGG+AGG + chr7.4:10650916-10650935 None:intergenic 55.0%
TCTTGCCAAACACTGCCTGG+TGG + chr7.4:10651929-10651948 None:intergenic 55.0%
TGCTGCAATGAGAGGACGTG+TGG - chr7.4:10650521-10650540 MS.gene061875:CDS 55.0%
TTCCTCTCACTAGCTGCACG+AGG + chr7.4:10650674-10650693 None:intergenic 55.0%
! GAAGCAGGCACTGCAGTTGT+GGG - chr7.4:10651995-10652014 MS.gene061875:CDS 55.0%
! TGAAGCAGGCACTGCAGTTG+TGG - chr7.4:10651994-10652013 MS.gene061875:CDS 55.0%
!! GTTGGTAGTGGCTGTGGTTG+TGG + chr7.4:10651701-10651720 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 10650192 10652332 10650192 ID=MS.gene061875
chr7.4 mRNA 10650192 10652332 10650192 ID=MS.gene061875.t1;Parent=MS.gene061875
chr7.4 exon 10651748 10652332 10651748 ID=MS.gene061875.t1.exon1;Parent=MS.gene061875.t1
chr7.4 CDS 10651748 10652332 10651748 ID=cds.MS.gene061875.t1;Parent=MS.gene061875.t1
chr7.4 exon 10650314 10650814 10650314 ID=MS.gene061875.t1.exon2;Parent=MS.gene061875.t1
chr7.4 CDS 10650314 10650814 10650314 ID=cds.MS.gene061875.t1;Parent=MS.gene061875.t1
chr7.4 exon 10650192 10650287 10650192 ID=MS.gene061875.t1.exon3;Parent=MS.gene061875.t1
chr7.4 CDS 10650192 10650287 10650192 ID=cds.MS.gene061875.t1;Parent=MS.gene061875.t1
Gene Sequence

>MS.gene061875

ATGAGAGAGCTCTACAATGCATCATTAAATGGAAGTGTAAGTTCCTTAAGAACATTGATCCAAAGAGACCCTCTCATTTTGAGCAAAGTTTCGTTGTATCCCTTCTCTGAAACACCATTGCACATAGCCTGTTTGCTTGGAAACTTCGAGTTTTGTCGGATTCTTCTTGATATAGATCCCAATTTAGCAGCTGAAGTGAACTTAGAAGGACGTTGCCCTCTTCATTTGGCTTCTGCAAAAGGGTACACTGAGATTGTGAGAGCAATATTGTTGACAAATCCAAAAACTTGCTTCATTAGGGACAAAGATGACAAGCTTCCTATCCACTTTGCTGCAATGAGAGGACGTGTGGAAGCAATTAAGGAATTGAATAGTGTCATGCCAGAAACCGAAATCATTAAGGTGATGTTTGAGACTGATCATCATGGTTCCATTCTACACTTGTGTGTTCGCTATAACCATCTAGAGGCTTTGAAAATCCTCGTGCAGCTAGTGAGAGGAAACAATAGATTACAATTCTTATATGTCAAAGACAAAGAAGGTAACACTGTTCTGCATTTAGCTGTCAGACGTGCACAAATAAAGGTACTGCTTGCCATTTTTCTAAAATAACTTGAATGTTTTCTCAACTATAGTATTTTCTCCAAATTATTTATATCAAATCACCTCTATTTTAATCTATAACAAACGGACTTATAGTTGACTCAATCAATACATTTTACCTCCCAACTCCAACTGTCTTGCTAGGTGAGTTGTGACCTGCCTGCATACCATTTTAGCTCACCCTGATTCTATCTGACCTTGTGTTCAAGTTGAGCCTTTGAGGCCTCAAGTCTGCTTTTTCCTTAATTCTTGTATATAAACCATTACTGTTATTGGGTTCTCATAGCAGGTTGAACTTTGAGAGCGACCCAATGTGAAGAGTATTGATTGAGATATCGATCCAAAACGACTAGACTTTATAGACTTGAAAATTTAAGGAAGAACTTCCCACGCACCGCAATATCACTTCAAATGTACATGTCATAAAAAAAATGTAAATTTTATGCCGTCTAAAAGAACACATTCATACAAAACATGTCATTCAAAAGTACACAAATGTGTGCCAAGTAAAAATGCTCCGAAAATGTATGTACACCTTCGCTCTCTTTAATTTATCCCGTATGAAACTTACATGTTCATCAACATAAAACAATAGAAAAGGCTCTACTAACTTGTGTAACATTGCAGATCATTAAATACTTGCTATCACTGTCTGAAATGAGCACAGCTATAAATAATTTGAACAACATGGGTTTAACACCATTGGATATGCTGGATCACAGTTCAAGAGATTTTGATTTTATTAGTCTTACAATCGATCATATGTTAGCTAAAGAAGGAGCTCAAAGGGCTACAAATATTGTCACAACACATCTACCAGCTTCCTCGTCAACTGAAGTTAACATACACCAAATGACAATGTCACAAAATATAGTTTCTGCACAACAAGATCATATGCTCTCGCCACAACCACAGCCACTACCAACACAAACATCACCAAAGAATTCACAGTCAACACAAACATCAAACACTACTAATGAACAACACAGACGCAACAGATGGAACATGTTTGAGAACTTCTGCACGACATACCTAATAAACCAAGGTAGTTGGATTGATAAAAAGACAAAAGAACAATTCATGGTAGCAGCAACTGTAATCGCAACAATGACATTTCAATCAGTAATAAGTCCACCAGGCAGTGTTTGGCAAGAAGATACAAAACACGGTAACCATGCATGCACTACTTATGATTTCTGTGAAGCAGGCACTGCAGTTGTGGGTTATGTTTGGTCACCAGATTTTATGAAATTTATATTTTTCAACTCTGCATCTTTCTTTGCTTCTCTGTGTGTGGTGTTGGTTCTCATCAGTGGATTTCCACTTGGGAATAAGGCCATGATGGGGATTTTAGCAGTTTTAATGATAATTGCTGTCACGTTCATGTTGCTCACATACATGTGGGCACTTGGATTGGTGAGTCCTAATCATGTTTATTACAGAATTCGCAAACTGGGATATGTTTTGGTTGGTACCTGGTCTTTCTTACTTCTAGTTGTTGGTTTGATTCAAATTACAAGAGTGGTGTTTTGGATTAG
Protein sequence

>MS.gene061875.t1

MRELYNASLNGSVSSLRTLIQRDPLILSKVSLYPFSETPLHIACLLGNFEFCRILLDIDPNLAAEVNLEGRCPLHLASAKGYTEIVRAILLTNPKTCFIRDKDDKLPIHFAAMRGRVEAIKELNSVMPETEIIKVMFETDHHGSILHLCVRYNHLEALKILVQLVRGNNRLQFLYVKDKEGNTVLHLAVRRAQIKPLPTQTSPKNSQSTQTSNTTNEQHRRNRWNMFENFCTTYLINQGSWIDKKTKEQFMVAATVIATMTFQSVISPPGSVWQEDTKHGNHACTTYDFCEAGTAVVGYVWSPDFMKFIFFNSASFFASLCVVLVLISGFPLGNKAMMGILAVLMIIAVTFMLLTYMWALGLNSQTGICFGWYLVFLTSSCWFDSNYKSGVLD