AlfalfaGEDB | MS.gene062158

M. sativa cultivar XinJiangDaYe / MS.gene062158

KEGG: K01761
GO: GO:0000096 GO:0003674 GO:0003824 GO:0005488 GO:0006082 GO:0006520 GO:0006534 GO:0006790 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009069 GO:0009092 GO:0009987 GO:0019346 GO:0019752 GO:0019842 GO:0030170 GO:0036094 GO:0043167 GO:0043168 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0048037 GO:0050662 GO:0050667 GO:0070279 GO:0071704 GO:0097159 GO:1901363 GO:1901564 GO:1901605
PFAM: PF01053.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062158.t1	XP_013468871.1	99.4	180	1	0	1	180	271	450	1.60E-98	368.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062158.t1	Q9SGU9	76.0	171	41	0	1	171	271	441	5.0e-72	271.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062158.t1	A0A072VNE2	99.4	180	1	0	1	180	271	450	1.2e-98	368.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene057694	MS.gene062158	0.80364	3.16E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062158.t1	MTR_1g077890	99.444	180	1	0	1	180	271	450	7.22e-133	379
MS.gene062158.t1	MTR_3g080850	83.333	168	28	0	6	173	274	441	5.37e-93	278

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062158.t1	AT1G64660	76.023	171	41	0	1	171	271	441	1.99e-85	258

Find 48 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGATAGTTCAAATGCTATTT+TGG	0.073928	1.2:+62737979	None:intergenic
AAAGTTATTCAATTCTTATT+AGG	0.141789	1.2:+62737503	None:intergenic
TTGCAAAACTATGGACAATT+TGG	0.197366	1.2:-62737731	MS.gene062158:CDS
CATGATGTGGATGGTCTTCT+AGG	0.248249	1.2:+62737852	None:intergenic
TGGTTTCATGGCTGTTAGTT+TGG	0.268438	1.2:-62737711	MS.gene062158:CDS
CTATGGACAATTTGGTTTCA+TGG	0.269872	1.2:-62737723	MS.gene062158:CDS
AAGTTATTCAATTCTTATTA+GGG	0.278319	1.2:+62737504	None:intergenic
ATGAGCTATTTGCAAAACTA+TGG	0.283875	1.2:-62737740	MS.gene062158:CDS
GGTTTCATGGCTGTTAGTTT+GGG	0.314829	1.2:-62737710	MS.gene062158:CDS
AGGATGTCAATAGGGTATAT+TGG	0.322482	1.2:-62737590	MS.gene062158:CDS
TGTTGGTCCTAATAGCATTA+TGG	0.326242	1.2:+62738009	None:intergenic
GTATCTAGACTTCAGGAATA+TGG	0.330404	1.2:-62737530	MS.gene062158:CDS
TCTGAGGAAAAGGTTCTTGC+TGG	0.335620	1.2:-62737632	MS.gene062158:CDS
CAAGGGGCCATAATGCTATT+AGG	0.360521	1.2:-62738016	MS.gene062158:CDS
GAGACACTTATGTCTTGTTC+TGG	0.369668	1.2:-62737680	MS.gene062158:CDS
TTGGGACTTTGGAGCAGAAA+TGG	0.390914	1.2:-62737571	MS.gene062158:CDS
TCAATTCACAACAAAGACTA+TGG	0.391666	1.2:-62737818	MS.gene062158:CDS
TGCTATGTTCTTTCATCCTT+AGG	0.394998	1.2:+62737936	None:intergenic
GGATGTCAATAGGGTATATT+GGG	0.402478	1.2:-62737589	MS.gene062158:CDS
AAAGGCAGTATCTAGACTTC+AGG	0.410254	1.2:-62737537	MS.gene062158:CDS
CACAACAAAGACTATGGTTA+TGG	0.412875	1.2:-62737812	MS.gene062158:CDS
GGTCTTGTGAGGATGTCAAT+AGG	0.421102	1.2:-62737599	MS.gene062158:CDS
AATAGGGTATATTGGGACTT+TGG	0.430260	1.2:-62737582	MS.gene062158:CDS
TGAGTTGAATTCTGAGGAAA+AGG	0.445451	1.2:-62737642	MS.gene062158:CDS
GTCTTGTGAGGATGTCAATA+GGG	0.477443	1.2:-62737598	MS.gene062158:CDS
CTAATAGCATTATGGCCCCT+TGG	0.496849	1.2:+62738017	None:intergenic
TGTGGATGGTCTTCTAGGCC+AGG	0.509408	1.2:+62737857	None:intergenic
GAAATGGAATCAGCTTGAAA+AGG	0.516409	1.2:-62737555	MS.gene062158:CDS
GAATCCCTCACTTAGGCCTA+AGG	0.525026	1.2:-62737952	MS.gene062158:CDS
GTTCTTGCTGGAATTTCACC+AGG	0.532752	1.2:-62737620	MS.gene062158:CDS
ATAGCATTATGGCCCCTTGG+TGG	0.543434	1.2:+62738020	None:intergenic
GTGGATGGTCTTCTAGGCCA+GGG	0.553045	1.2:+62737858	None:intergenic
TCTTTGCATTGACATGGAAA+CGG	0.553898	1.2:-62737783	MS.gene062158:CDS
GATTTCAACAATTCATGATG+TGG	0.554996	1.2:+62737839	None:intergenic
TCAACAATTCATGATGTGGA+TGG	0.562975	1.2:+62737843	None:intergenic
TGGACTTCTTTGCATTGACA+TGG	0.579725	1.2:-62737789	MS.gene062158:CDS
AACAAAGACTATGGTTATGG+TGG	0.583091	1.2:-62737809	MS.gene062158:CDS
CTATGATGGATCTCCACCAA+GGG	0.585796	1.2:-62738033	MS.gene062158:CDS
ATTGACATCCTCACAAGACC+TGG	0.585919	1.2:+62737602	None:intergenic
GAATTTCACCAGGTCTTGTG+AGG	0.587396	1.2:-62737610	MS.gene062158:CDS
TCAGAAAGAATCCCTCACTT+AGG	0.590587	1.2:-62737959	MS.gene062158:CDS
GGATTCAAGGTAATCTACCC+TGG	0.598316	1.2:-62737875	MS.gene062158:CDS
TCATCCTTAGGCCTAAGTGA+GGG	0.616919	1.2:+62737948	None:intergenic
TTCATCCTTAGGCCTAAGTG+AGG	0.628773	1.2:+62737947	None:intergenic
TCTATGATGGATCTCCACCA+AGG	0.633733	1.2:-62738034	MS.gene062158:CDS
TAGTAGTGAGTTGAATTCTG+AGG	0.646330	1.2:-62737648	MS.gene062158:CDS
TTGTTGCAAACACTAATGCA+CGG	0.650041	1.2:+62737912	None:intergenic
TATGATGGATCTCCACCAAG+GGG	0.715539	1.2:-62738032	MS.gene062158:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACTAAAAAAATTAGGATTCA+AGG	-	chr1.2:62737654-62737673	MS.gene062158:CDS	20.0%
!	GCAACAAGACTAAAAAAATT+AGG	-	chr1.2:62737646-62737665	MS.gene062158:CDS	25.0%
!!!	ATAGTTCAAATGCTATTTTG+GGG	+	chr1.2:62737564-62737583	None:intergenic	25.0%
!!!	GATAGTTCAAATGCTATTTT+GGG	+	chr1.2:62737565-62737584	None:intergenic	25.0%
!!!	TGATAGTTCAAATGCTATTT+TGG	+	chr1.2:62737566-62737585	None:intergenic	25.0%
	ATGAGCTATTTGCAAAACTA+TGG	-	chr1.2:62737802-62737821	MS.gene062158:CDS	30.0%
	GATTTCAACAATTCATGATG+TGG	+	chr1.2:62737706-62737725	None:intergenic	30.0%
	TCAATTCACAACAAAGACTA+TGG	-	chr1.2:62737724-62737743	MS.gene062158:CDS	30.0%
	TTGCAAAACTATGGACAATT+TGG	-	chr1.2:62737811-62737830	MS.gene062158:CDS	30.0%
	AACAAAGACTATGGTTATGG+TGG	-	chr1.2:62737733-62737752	MS.gene062158:CDS	35.0%
	AATAGGGTATATTGGGACTT+TGG	-	chr1.2:62737960-62737979	MS.gene062158:CDS	35.0%
	AGGATGTCAATAGGGTATAT+TGG	-	chr1.2:62737952-62737971	MS.gene062158:CDS	35.0%
	CACAACAAAGACTATGGTTA+TGG	-	chr1.2:62737730-62737749	MS.gene062158:CDS	35.0%
	GAAATGGAATCAGCTTGAAA+AGG	-	chr1.2:62737987-62738006	MS.gene062158:CDS	35.0%
	GGATGTCAATAGGGTATATT+GGG	-	chr1.2:62737953-62737972	MS.gene062158:CDS	35.0%
	GTATCTAGACTTCAGGAATA+TGG	-	chr1.2:62738012-62738031	MS.gene062158:CDS	35.0%
	TCAACAATTCATGATGTGGA+TGG	+	chr1.2:62737702-62737721	None:intergenic	35.0%
	TGCTATGTTCTTTCATCCTT+AGG	+	chr1.2:62737609-62737628	None:intergenic	35.0%
	TGTTGGTCCTAATAGCATTA+TGG	+	chr1.2:62737536-62737555	None:intergenic	35.0%
	TTGTTGCAAACACTAATGCA+CGG	+	chr1.2:62737633-62737652	None:intergenic	35.0%
!	CTATGGACAATTTGGTTTCA+TGG	-	chr1.2:62737819-62737838	MS.gene062158:CDS	35.0%
!	TAGTAGTGAGTTGAATTCTG+AGG	-	chr1.2:62737894-62737913	MS.gene062158:CDS	35.0%
!	TCTTTGCATTGACATGGAAA+CGG	-	chr1.2:62737759-62737778	MS.gene062158:CDS	35.0%
!	TGAGTTGAATTCTGAGGAAA+AGG	-	chr1.2:62737900-62737919	MS.gene062158:CDS	35.0%
	AAAGGCAGTATCTAGACTTC+AGG	-	chr1.2:62738005-62738024	MS.gene062158:CDS	40.0%
	GTCTTGTGAGGATGTCAATA+GGG	-	chr1.2:62737944-62737963	MS.gene062158:CDS	40.0%
	TCAGAAAGAATCCCTCACTT+AGG	-	chr1.2:62737583-62737602	MS.gene062158:CDS	40.0%
	TGGTTTCATGGCTGTTAGTT+TGG	-	chr1.2:62737831-62737850	MS.gene062158:CDS	40.0%
!	GAGACACTTATGTCTTGTTC+TGG	-	chr1.2:62737862-62737881	MS.gene062158:CDS	40.0%
!	GGTTTCATGGCTGTTAGTTT+GGG	-	chr1.2:62737832-62737851	MS.gene062158:CDS	40.0%
!	TGGACTTCTTTGCATTGACA+TGG	-	chr1.2:62737753-62737772	MS.gene062158:CDS	40.0%
!!!	GCTATTTTGGGGTTCATTGT+TGG	+	chr1.2:62737553-62737572	None:intergenic	40.0%
	ATTGACATCCTCACAAGACC+TGG	+	chr1.2:62737943-62737962	None:intergenic	45.0%
	CAAGGGGCCATAATGCTATT+AGG	-	chr1.2:62737526-62737545	MS.gene062158:CDS	45.0%
	CATGATGTGGATGGTCTTCT+AGG	+	chr1.2:62737693-62737712	None:intergenic	45.0%
	CTAATAGCATTATGGCCCCT+TGG	+	chr1.2:62737528-62737547	None:intergenic	45.0%
	GGATTCAAGGTAATCTACCC+TGG	-	chr1.2:62737667-62737686	MS.gene062158:CDS	45.0%
	GTTCTTGCTGGAATTTCACC+AGG	-	chr1.2:62737922-62737941	MS.gene062158:CDS	45.0%
	TCATCCTTAGGCCTAAGTGA+GGG	+	chr1.2:62737597-62737616	None:intergenic	45.0%
	TCTGAGGAAAAGGTTCTTGC+TGG	-	chr1.2:62737910-62737929	MS.gene062158:CDS	45.0%
	TTCATCCTTAGGCCTAAGTG+AGG	+	chr1.2:62737598-62737617	None:intergenic	45.0%
!	GAATTTCACCAGGTCTTGTG+AGG	-	chr1.2:62737932-62737951	MS.gene062158:CDS	45.0%
!	GGTCTTGTGAGGATGTCAAT+AGG	-	chr1.2:62737943-62737962	MS.gene062158:CDS	45.0%
!!	TTGGGACTTTGGAGCAGAAA+TGG	-	chr1.2:62737971-62737990	MS.gene062158:CDS	45.0%
	ATAGCATTATGGCCCCTTGG+TGG	+	chr1.2:62737525-62737544	None:intergenic	50.0%
	GAATCCCTCACTTAGGCCTA+AGG	-	chr1.2:62737590-62737609	MS.gene062158:CDS	50.0%
	GTGGATGGTCTTCTAGGCCA+GGG	+	chr1.2:62737687-62737706	None:intergenic	55.0%
	TGTGGATGGTCTTCTAGGCC+AGG	+	chr1.2:62737688-62737707	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	62737511	62738053	62737511	ID=MS.gene062158
chr1.2	mRNA	62737511	62738053	62737511	ID=MS.gene062158.t1;Parent=MS.gene062158
chr1.2	exon	62737511	62738053	62737511	ID=MS.gene062158.t1.exon1;Parent=MS.gene062158.t1
chr1.2	CDS	62737511	62738053	62737511	ID=cds.MS.gene062158.t1;Parent=MS.gene062158.t1

Gene Sequence

>MS.gene062158

ATGATGGATCTCCACCAAGGGGCCATAATGCTATTAGGACCAACAATGAACCCCAAAATAGCATTTGAACTATCAGAAAGAATCCCTCACTTAGGCCTAAGGATGAAAGAACATAGCAACCGTGCATTAGTGTTTGCAACAAGACTAAAAAAATTAGGATTCAAGGTAATCTACCCTGGCCTAGAAGACCATCCACATCATGAATTGTTGAAATCAATTCACAACAAAGACTATGGTTATGGTGGACTTCTTTGCATTGACATGGAAACGGAAGAAAGAGCTAACAAATTGATGAGCTATTTGCAAAACTATGGACAATTTGGTTTCATGGCTGTTAGTTTGGGATACTATGAGACACTTATGTCTTGTTCTGGAAGTAGCACTAGTAGTGAGTTGAATTCTGAGGAAAAGGTTCTTGCTGGAATTTCACCAGGTCTTGTGAGGATGTCAATAGGGTATATTGGGACTTTGGAGCAGAAATGGAATCAGCTTGAAAAGGCAGTATCTAGACTTCAGGAATATGGTTTCCCTAATAAGAATTGA

Protein sequence

>MS.gene062158.t1

MMDLHQGAIMLLGPTMNPKIAFELSERIPHLGLRMKEHSNRALVFATRLKKLGFKVIYPGLEDHPHHELLKSIHNKDYGYGGLLCIDMETEERANKLMSYLQNYGQFGFMAVSLGYYETLMSCSGSSTSSELNSEEKVLAGISPGLVRMSIGYIGTLEQKWNQLEKAVSRLQEYGFPNKN