Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06241.t1 | XP_003602781.1 | 98.4 | 369 | 6 | 0 | 1 | 369 | 12 | 380 | 2.20E-214 | 754.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06241.t1 | Q0E0Y3 | 69.0 | 377 | 106 | 3 | 3 | 369 | 8 | 383 | 3.8e-151 | 535.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06241.t1 | G7J3D1 | 98.4 | 369 | 6 | 0 | 1 | 369 | 12 | 380 | 1.6e-214 | 754.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene06241 | MS.gene005659 | PPI |
| MS.gene06241 | MS.gene07153 | PPI |
| MS.gene02998 | MS.gene06241 | PPI |
| MS.gene21545 | MS.gene06241 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06241.t1 | MTR_3g098940 | 98.374 | 369 | 6 | 0 | 1 | 369 | 12 | 380 | 0.0 | 757 |
| MS.gene06241.t1 | MTR_3g098940 | 98.225 | 338 | 6 | 0 | 1 | 338 | 12 | 349 | 0.0 | 686 |
| MS.gene06241.t1 | MTR_1g095580 | 26.616 | 263 | 163 | 7 | 42 | 275 | 29 | 290 | 1.15e-16 | 80.5 |
| MS.gene06241.t1 | MTR_1g095580 | 26.616 | 263 | 163 | 7 | 42 | 275 | 29 | 290 | 1.96e-16 | 79.7 |
| MS.gene06241.t1 | MTR_1g095580 | 25.490 | 306 | 185 | 9 | 42 | 313 | 29 | 325 | 4.51e-16 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06241.t1 | AT5G51140 | 68.785 | 362 | 93 | 4 | 16 | 369 | 46 | 395 | 3.51e-179 | 503 |
| MS.gene06241.t1 | AT5G51140 | 68.555 | 353 | 91 | 4 | 16 | 360 | 46 | 386 | 3.06e-172 | 486 |
| MS.gene06241.t1 | AT3G52260 | 25.000 | 300 | 193 | 7 | 45 | 313 | 34 | 332 | 7.08e-18 | 84.3 |
| MS.gene06241.t1 | AT3G52260 | 26.136 | 264 | 164 | 6 | 45 | 277 | 34 | 297 | 1.97e-17 | 82.8 |
| MS.gene06241.t1 | AT3G52260 | 25.581 | 258 | 161 | 6 | 45 | 271 | 34 | 291 | 3.50e-15 | 75.9 |
| MS.gene06241.t1 | AT3G52260 | 25.984 | 254 | 151 | 6 | 71 | 294 | 91 | 337 | 9.42e-13 | 69.3 |
Find 86 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACCCCTTCGGTGCCAAATT+TGG | 0.184524 | 3.1:+78817717 | None:intergenic |
| CGCGTTCATTTACAGTCTTC+TGG | 0.226994 | 3.1:-78817933 | MS.gene06241:CDS |
| TAGCATTTCTGGCTATAATA+AGG | 0.255944 | 3.1:+78821596 | None:intergenic |
| GTGTACCAACTGTCCAAATT+TGG | 0.306598 | 3.1:-78817730 | MS.gene06241:CDS |
| AGTTTCGCCCAATCAGGATA+TGG | 0.308758 | 3.1:+78817006 | None:intergenic |
| TTATTCGCTGAGGAATTTAA+AGG | 0.319656 | 3.1:-78823749 | MS.gene06241:CDS |
| TCGAGCATTATAGTCTATAT+TGG | 0.345385 | 3.1:+78819835 | None:intergenic |
| GCTTCTTTGACATCAAATTT+CGG | 0.348641 | 3.1:-78817813 | MS.gene06241:CDS |
| TGCGGTAAAGGAGGATTTGC+TGG | 0.374155 | 3.1:+78824061 | None:intergenic |
| AATATATTGCAAAAGTAGTT+GGG | 0.377867 | 3.1:-78820710 | MS.gene06241:CDS |
| TACAGCATGTCATTGGCTAT+TGG | 0.388217 | 3.1:+78817906 | None:intergenic |
| GAGTACCATTGCTACTAATC+CGG | 0.396097 | 3.1:+78818542 | None:intergenic |
| CAATATATTGCAAAAGTAGT+TGG | 0.398952 | 3.1:-78820711 | MS.gene06241:CDS |
| CCAGCCCATGTTCTGCTTGA+AGG | 0.399831 | 3.1:+78821937 | None:intergenic |
| ACTGTTGATAGATCTATTAA+TGG | 0.400312 | 3.1:-78817870 | MS.gene06241:CDS |
| ATGAATGCCCATATCCTGAT+TGG | 0.401822 | 3.1:-78817014 | MS.gene06241:CDS |
| ATCAATGCTAAACTCCTCAT+TGG | 0.413066 | 3.1:+78817758 | None:intergenic |
| TTACAAACAGTTAGCACATC+TGG | 0.415839 | 3.1:+78823326 | None:intergenic |
| AGCTAAAGTTTCGCCCAATC+AGG | 0.416919 | 3.1:+78817000 | None:intergenic |
| ACGATGTAATCTTGCGGTAA+AGG | 0.424137 | 3.1:+78824049 | None:intergenic |
| TCTGGTTCTGTATGAAGAAT+AGG | 0.424198 | 3.1:+78823344 | None:intergenic |
| ACAGCATGTCATTGGCTATT+GGG | 0.433925 | 3.1:+78817907 | None:intergenic |
| TATCGCAAGAACACTGTTGT+TGG | 0.444093 | 3.1:-78821963 | MS.gene06241:CDS |
| AAGGTCAGAGACTCTACAAA+AGG | 0.453239 | 3.1:-78818589 | MS.gene06241:CDS |
| CGGTGCCAAATTTGGACAGT+TGG | 0.457022 | 3.1:+78817725 | None:intergenic |
| AGTCACTGGCCGCACTCATC+AGG | 0.471463 | 3.1:-78818497 | MS.gene06241:intron |
| GATTTAACTACGTATGAAAC+CGG | 0.475395 | 3.1:+78823501 | None:intergenic |
| TTACTTACCGGGACAGATGC+AGG | 0.477862 | 3.1:+78823302 | None:intergenic |
| CTGTCCAAATTTGGCACCGA+AGG | 0.478848 | 3.1:-78817721 | MS.gene06241:CDS |
| CTGTTGATAGATCTATTAAT+GGG | 0.482702 | 3.1:-78817869 | MS.gene06241:CDS |
| CCTTCAAGCAGAACATGGGC+TGG | 0.487356 | 3.1:-78821937 | MS.gene06241:CDS |
| AAACTTACATAATATTCATA+AGG | 0.492762 | 3.1:+78823722 | None:intergenic |
| CGACTGGATCGCCTTGTCTC+CGG | 0.493010 | 3.1:-78821622 | MS.gene06241:CDS |
| TGATGAGTGCGGCCAGTGAC+TGG | 0.494032 | 3.1:+78818499 | None:intergenic |
| TGAGATGTACAGCATGTCAT+TGG | 0.495292 | 3.1:+78817899 | None:intergenic |
| TAAAGAATTGCATACCCCTT+CGG | 0.504219 | 3.1:+78817705 | None:intergenic |
| GCGATGAATTCGAAGTAGTA+TGG | 0.504359 | 3.1:+78823912 | None:intergenic |
| GTTTCGCCCAATCAGGATAT+GGG | 0.504686 | 3.1:+78817007 | None:intergenic |
| TGAATGCCCATATCCTGATT+GGG | 0.506186 | 3.1:-78817013 | MS.gene06241:CDS |
| TTACATTGTATGCGGTACTC+TGG | 0.510906 | 3.1:-78817051 | MS.gene06241:CDS |
| CAAATAAAATACCTCATCCT+CGG | 0.512397 | 3.1:+78820680 | None:intergenic |
| AAATAAAATACCTCATCCTC+GGG | 0.514851 | 3.1:+78820681 | None:intergenic |
| GGCATCCTTCAAGCAGAACA+TGG | 0.516228 | 3.1:-78821942 | MS.gene06241:CDS |
| TGTCTCAGCTGTCCATCGAC+TGG | 0.517338 | 3.1:-78821638 | MS.gene06241:intron |
| TGGGGAATTTCCCGAGGATG+AGG | 0.518507 | 3.1:-78820691 | MS.gene06241:intron |
| CAGGCATGAACCACCTGTGA+TGG | 0.518717 | 3.1:-78823378 | MS.gene06241:CDS |
| TCTGGCTATAATAAGGAGTC+CGG | 0.521448 | 3.1:+78821603 | None:intergenic |
| AGTACCATTGCTACTAATCC+GGG | 0.522821 | 3.1:+78818543 | None:intergenic |
| TGGTTGTTACAGATTGAGGC+TGG | 0.531299 | 3.1:-78820747 | MS.gene06241:intron |
| AGATATGATACTGATGAAGA+AGG | 0.536074 | 3.1:-78817081 | MS.gene06241:CDS |
| TTGTAAGCCTGCATCTGTCC+CGG | 0.551212 | 3.1:-78823309 | MS.gene06241:intron |
| GGGCATTCATATGTCCATCC+AGG | 0.555137 | 3.1:+78817027 | None:intergenic |
| TAGGTTAGTGCGGTGAAATG+TGG | 0.563936 | 3.1:-78823552 | MS.gene06241:CDS |
| TGTCCAAATTTGGCACCGAA+GGG | 0.570374 | 3.1:-78817720 | MS.gene06241:CDS |
| AGGTCAGAGACTCTACAAAA+GGG | 0.571744 | 3.1:-78818588 | MS.gene06241:CDS |
| TGTGGAAGGATTCAAGTTGA+CGG | 0.572120 | 3.1:-78823534 | MS.gene06241:CDS |
| CAAGATTACATCGTTCGAAA+CGG | 0.576471 | 3.1:-78824040 | MS.gene06241:intron |
| TTGCCTCCACAAAATTTACC+CGG | 0.581737 | 3.1:-78818561 | MS.gene06241:CDS |
| TGCGGTACTCTGGACCTGGA+TGG | 0.588015 | 3.1:-78817041 | MS.gene06241:CDS |
| ATACTGATGAAGAAGGTTTG+TGG | 0.590363 | 3.1:-78817074 | MS.gene06241:CDS |
| AGACAAGGCGATCCAGTCGA+TGG | 0.592536 | 3.1:+78821626 | None:intergenic |
| ATAGACTATAATGCTCGAGA+AGG | 0.597554 | 3.1:-78819830 | MS.gene06241:CDS |
| TGTATGCGGTACTCTGGACC+TGG | 0.597884 | 3.1:-78817045 | MS.gene06241:CDS |
| TAATAAGGAGTCCGGAGACA+AGG | 0.599949 | 3.1:+78821611 | None:intergenic |
| TTTACCCGGATTAGTAGCAA+TGG | 0.600058 | 3.1:-78818547 | MS.gene06241:CDS |
| GGAACATCACAAGCCATCAC+AGG | 0.601035 | 3.1:+78823365 | None:intergenic |
| AGAAGGAAGAAGCACAGCAG+AGG | 0.605852 | 3.1:-78819813 | MS.gene06241:intron |
| ATATATTGCAAAAGTAGTTG+GGG | 0.609841 | 3.1:-78820709 | MS.gene06241:CDS |
| TTAGTGCGGTGAAATGTGGA+AGG | 0.615192 | 3.1:-78823548 | MS.gene06241:CDS |
| AGCCAGAAATGCTACAAAAG+CGG | 0.615290 | 3.1:-78821587 | MS.gene06241:CDS |
| ATGTAATCTTGCGGTAAAGG+AGG | 0.615710 | 3.1:+78824052 | None:intergenic |
| TATTGTAGATTTATTCGCTG+AGG | 0.616478 | 3.1:-78823759 | MS.gene06241:CDS |
| TTTGGACAGTTGGTACACAT+AGG | 0.616779 | 3.1:+78817735 | None:intergenic |
| AGTTGACGGTGAAATCGTGC+CGG | 0.620508 | 3.1:-78823520 | MS.gene06241:CDS |
| CAGAGACTCTACAAAAGGGA+AGG | 0.630227 | 3.1:-78818584 | MS.gene06241:CDS |
| GTATGGATCTTAGGTTAGTG+CGG | 0.630653 | 3.1:-78823562 | MS.gene06241:intron |
| GTTTGTGGTTACATTGTATG+CGG | 0.636819 | 3.1:-78817059 | MS.gene06241:CDS |
| GTCCAAATTTGGCACCGAAG+GGG | 0.645456 | 3.1:-78817719 | MS.gene06241:intron |
| GCATCCTTCAAGCAGAACAT+GGG | 0.646483 | 3.1:-78821941 | MS.gene06241:CDS |
| TTCTTGCGATATTGACCACA+TGG | 0.665528 | 3.1:+78821975 | None:intergenic |
| TTGTCAAGAAAATTCCAATG+AGG | 0.670627 | 3.1:-78817772 | MS.gene06241:CDS |
| ACATCACAAGCCATCACAGG+TGG | 0.674685 | 3.1:+78823368 | None:intergenic |
| AGTAGTTGGGGAATTTCCCG+AGG | 0.679238 | 3.1:-78820697 | MS.gene06241:CDS |
| TTTCGAACGATGTAATCTTG+CGG | 0.684194 | 3.1:+78824043 | None:intergenic |
| AAGAGTGTATGGATATCGTG+TGG | 0.692668 | 3.1:-78824090 | None:intergenic |
| GGTACATAGGTTCATCCATG+TGG | 0.702272 | 3.1:-78821990 | MS.gene06241:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 78817002 | 78824104 | 78817002 | ID=MS.gene06241 |
| chr3.1 | mRNA | 78817002 | 78824104 | 78817002 | ID=MS.gene06241.t1;Parent=MS.gene06241 |
| chr3.1 | exon | 78824041 | 78824104 | 78824041 | ID=MS.gene06241.t1.exon1;Parent=MS.gene06241.t1 |
| chr3.1 | CDS | 78824041 | 78824104 | 78824041 | ID=cds.MS.gene06241.t1;Parent=MS.gene06241.t1 |
| chr3.1 | exon | 78823908 | 78823951 | 78823908 | ID=MS.gene06241.t1.exon2;Parent=MS.gene06241.t1 |
| chr3.1 | CDS | 78823908 | 78823951 | 78823908 | ID=cds.MS.gene06241.t1;Parent=MS.gene06241.t1 |
| chr3.1 | exon | 78823730 | 78823807 | 78823730 | ID=MS.gene06241.t1.exon3;Parent=MS.gene06241.t1 |
| chr3.1 | CDS | 78823730 | 78823807 | 78823730 | ID=cds.MS.gene06241.t1;Parent=MS.gene06241.t1 |
| chr3.1 | exon | 78823471 | 78823571 | 78823471 | ID=MS.gene06241.t1.exon4;Parent=MS.gene06241.t1 |
| chr3.1 | CDS | 78823471 | 78823571 | 78823471 | ID=cds.MS.gene06241.t1;Parent=MS.gene06241.t1 |
| chr3.1 | exon | 78823310 | 78823397 | 78823310 | ID=MS.gene06241.t1.exon5;Parent=MS.gene06241.t1 |
| chr3.1 | CDS | 78823310 | 78823397 | 78823310 | ID=cds.MS.gene06241.t1;Parent=MS.gene06241.t1 |
| chr3.1 | exon | 78821925 | 78822003 | 78821925 | ID=MS.gene06241.t1.exon6;Parent=MS.gene06241.t1 |
| chr3.1 | CDS | 78821925 | 78822003 | 78821925 | ID=cds.MS.gene06241.t1;Parent=MS.gene06241.t1 |
| chr3.1 | exon | 78821570 | 78821652 | 78821570 | ID=MS.gene06241.t1.exon7;Parent=MS.gene06241.t1 |
| chr3.1 | CDS | 78821570 | 78821652 | 78821570 | ID=cds.MS.gene06241.t1;Parent=MS.gene06241.t1 |
| chr3.1 | exon | 78820692 | 78820757 | 78820692 | ID=MS.gene06241.t1.exon8;Parent=MS.gene06241.t1 |
| chr3.1 | CDS | 78820692 | 78820757 | 78820692 | ID=cds.MS.gene06241.t1;Parent=MS.gene06241.t1 |
| chr3.1 | exon | 78819814 | 78819870 | 78819814 | ID=MS.gene06241.t1.exon9;Parent=MS.gene06241.t1 |
| chr3.1 | CDS | 78819814 | 78819870 | 78819814 | ID=cds.MS.gene06241.t1;Parent=MS.gene06241.t1 |
| chr3.1 | exon | 78818498 | 78818608 | 78818498 | ID=MS.gene06241.t1.exon10;Parent=MS.gene06241.t1 |
| chr3.1 | CDS | 78818498 | 78818608 | 78818498 | ID=cds.MS.gene06241.t1;Parent=MS.gene06241.t1 |
| chr3.1 | exon | 78817720 | 78817958 | 78817720 | ID=MS.gene06241.t1.exon11;Parent=MS.gene06241.t1 |
| chr3.1 | CDS | 78817720 | 78817958 | 78817720 | ID=cds.MS.gene06241.t1;Parent=MS.gene06241.t1 |
| chr3.1 | exon | 78817002 | 78817101 | 78817002 | ID=MS.gene06241.t1.exon12;Parent=MS.gene06241.t1 |
| chr3.1 | CDS | 78817002 | 78817101 | 78817002 | ID=cds.MS.gene06241.t1;Parent=MS.gene06241.t1 |
| Gene Sequence |
| Protein sequence |
