Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062513.t1 | XP_003618038.1 | 78.7 | 75 | 15 | 1 | 1 | 74 | 1 | 75 | 1.70E-22 | 114.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062513.t1 | G7K0L2 | 78.7 | 75 | 15 | 1 | 1 | 74 | 1 | 75 | 1.3e-22 | 114.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049418 | MS.gene062513 | 0.842207 | 2.98E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062513.t1 | MTR_5g098300 | 88.000 | 75 | 8 | 1 | 1 | 74 | 1 | 75 | 1.80e-30 | 102 |
| MS.gene062513.t1 | MTR_5g098270 | 79.245 | 53 | 10 | 1 | 6 | 57 | 1 | 53 | 1.16e-23 | 84.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062513.t1 | AT1G31335 | 80.488 | 41 | 8 | 0 | 1 | 41 | 1 | 41 | 1.06e-19 | 75.1 |
Find 26 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACACAAACCCTCAAGTTTA+TGG | 0.161657 | 5.1:-78772724 | MS.gene062513:CDS |
| ATCTTCTTCTGCTTAATTCT+TGG | 0.163666 | 5.1:-78772793 | MS.gene062513:CDS |
| TTCTGCTTAATTCTTGGTTT+TGG | 0.239621 | 5.1:-78772787 | MS.gene062513:CDS |
| ATTAAGCAGAAGAAGATTAA+AGG | 0.258762 | 5.1:+78772799 | None:intergenic |
| GGAGCACCTGGTGGTGGAAT+TGG | 0.383062 | 5.1:+78772697 | None:intergenic |
| GTTGGAAGCAAGGTTTGGTT+TGG | 0.389775 | 5.1:+78772630 | None:intergenic |
| CAAACGTTGGAAGCAAGGTT+TGG | 0.414160 | 5.1:+78772625 | None:intergenic |
| GAAGGGAAGGTAGTATTTGC+AGG | 0.442054 | 5.1:+78772676 | None:intergenic |
| TTGGCATACCATAAACTTGA+GGG | 0.504350 | 5.1:+78772716 | None:intergenic |
| GAACTCAAACGTTGGAAGCA+AGG | 0.528191 | 5.1:+78772620 | None:intergenic |
| AATTAAGATGGGAATGGTAG+TGG | 0.531670 | 5.1:-78772833 | None:intergenic |
| GTAGTATTTGCAGGAGCACC+TGG | 0.535931 | 5.1:+78772685 | None:intergenic |
| TTTGCAGGAGCACCTGGTGG+TGG | 0.537498 | 5.1:+78772691 | None:intergenic |
| GGTTTGGAAAATTGTGGTGG+AGG | 0.551320 | 5.1:+78772646 | None:intergenic |
| TGGAGGAGATGATGAAGGGA+AGG | 0.551662 | 5.1:+78772663 | None:intergenic |
| TACTTCTTTGGAAGAGCTAG+AGG | 0.553790 | 5.1:-78772760 | MS.gene062513:CDS |
| TATGAGTTGAACTCAAACGT+TGG | 0.555554 | 5.1:+78772612 | None:intergenic |
| TGTGGTGGAGGAGATGATGA+AGG | 0.573146 | 5.1:+78772658 | None:intergenic |
| AGAGAAAATTAAGATGGGAA+TGG | 0.575884 | 5.1:-78772839 | None:intergenic |
| AGGTTTGGTTTGGAAAATTG+TGG | 0.581383 | 5.1:+78772640 | None:intergenic |
| TTTGGTTTGGAAAATTGTGG+TGG | 0.586635 | 5.1:+78772643 | None:intergenic |
| GGTATGCCAATTCCACCACC+AGG | 0.644332 | 5.1:-78772703 | MS.gene062513:CDS |
| GTGGTGGAGGAGATGATGAA+GGG | 0.655536 | 5.1:+78772659 | None:intergenic |
| ATTGGCATACCATAAACTTG+AGG | 0.668164 | 5.1:+78772715 | None:intergenic |
| GTATTTGCAGGAGCACCTGG+TGG | 0.685211 | 5.1:+78772688 | None:intergenic |
| GGAAGAGCTAGAGGAAGACG+TGG | 0.708953 | 5.1:-78772751 | MS.gene062513:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTAAGCAGAAGAAGATTAA+AGG | + | chr5.1:78772654-78772673 | None:intergenic | 25.0% |
| ATCTTCTTCTGCTTAATTCT+TGG | - | chr5.1:78772657-78772676 | MS.gene062513:CDS | 30.0% | |
| ! | TTCTGCTTAATTCTTGGTTT+TGG | - | chr5.1:78772663-78772682 | MS.gene062513:CDS | 30.0% |
| ATTGGCATACCATAAACTTG+AGG | + | chr5.1:78772738-78772757 | None:intergenic | 35.0% | |
| TACACAAACCCTCAAGTTTA+TGG | - | chr5.1:78772726-78772745 | MS.gene062513:CDS | 35.0% | |
| TTGGCATACCATAAACTTGA+GGG | + | chr5.1:78772737-78772756 | None:intergenic | 35.0% | |
| !! | AGGTTTGGTTTGGAAAATTG+TGG | + | chr5.1:78772813-78772832 | None:intergenic | 35.0% |
| !! | TTTGGTTTGGAAAATTGTGG+TGG | + | chr5.1:78772810-78772829 | None:intergenic | 35.0% |
| !!! | GGTTTTGGTTGTTACTTCTT+TGG | - | chr5.1:78772678-78772697 | MS.gene062513:CDS | 35.0% |
| ! | TACTTCTTTGGAAGAGCTAG+AGG | - | chr5.1:78772690-78772709 | MS.gene062513:CDS | 40.0% |
| CAAACGTTGGAAGCAAGGTT+TGG | + | chr5.1:78772828-78772847 | None:intergenic | 45.0% | |
| GAAGGGAAGGTAGTATTTGC+AGG | + | chr5.1:78772777-78772796 | None:intergenic | 45.0% | |
| GGTTTGGAAAATTGTGGTGG+AGG | + | chr5.1:78772807-78772826 | None:intergenic | 45.0% | |
| ! | GTTGGAAGCAAGGTTTGGTT+TGG | + | chr5.1:78772823-78772842 | None:intergenic | 45.0% |
| TGGAGGAGATGATGAAGGGA+AGG | + | chr5.1:78772790-78772809 | None:intergenic | 50.0% | |
| ! | GTGGTGGAGGAGATGATGAA+GGG | + | chr5.1:78772794-78772813 | None:intergenic | 50.0% |
| ! | TGTGGTGGAGGAGATGATGA+AGG | + | chr5.1:78772795-78772814 | None:intergenic | 50.0% |
| !! | GTAGTATTTGCAGGAGCACC+TGG | + | chr5.1:78772768-78772787 | None:intergenic | 50.0% |
| GGAAGAGCTAGAGGAAGACG+TGG | - | chr5.1:78772699-78772718 | MS.gene062513:CDS | 55.0% | |
| GGTATGCCAATTCCACCACC+AGG | - | chr5.1:78772747-78772766 | MS.gene062513:CDS | 55.0% | |
| !! | GTATTTGCAGGAGCACCTGG+TGG | + | chr5.1:78772765-78772784 | None:intergenic | 55.0% |
| !! | GGAGCACCTGGTGGTGGAAT+TGG | + | chr5.1:78772756-78772775 | None:intergenic | 60.0% |
| !! | TTTGCAGGAGCACCTGGTGG+TGG | + | chr5.1:78772762-78772781 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 78772624 | 78772848 | 78772624 | ID=MS.gene062513 |
| chr5.1 | mRNA | 78772624 | 78772848 | 78772624 | ID=MS.gene062513.t1;Parent=MS.gene062513 |
| chr5.1 | exon | 78772624 | 78772848 | 78772624 | ID=MS.gene062513.t1.exon1;Parent=MS.gene062513.t1 |
| chr5.1 | CDS | 78772624 | 78772848 | 78772624 | ID=cds.MS.gene062513.t1;Parent=MS.gene062513.t1 |
| Gene Sequence |
| Protein sequence |
