AlfalfaGEDB | MS.gene062744

M. sativa cultivar XinJiangDaYe / MS.gene062744

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062744.t1	XP_003589266.1	95.6	183	8	0	3	185	30	212	1.70E-98	368.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062744.t1	Q8L3W1	36.4	118	63	3	3	112	21	134	3.5e-12	73.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062744.t1	G7I4K0	95.6	183	8	0	3	185	30	212	1.2e-98	368.6

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene062744.t1	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene062744	0.806091	9.73E-50	-1.69E-46
MS.gene052609	MS.gene062744	0.825695	4.07E-54	-1.69E-46
MS.gene053079	MS.gene062744	0.809039	2.30E-50	-1.69E-46
MS.gene058010	MS.gene062744	0.81516	1.07E-51	-1.69E-46
MS.gene058919	MS.gene062744	0.804249	2.36E-49	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062744.t1	MTR_1g021270	95.628	183	8	0	3	185	30	212	6.67e-128	372
MS.gene062744.t1	MTR_4g068320	40.741	81	48	0	3	83	21	101	2.98e-16	75.9
MS.gene062744.t1	MTR_1g021320	40.698	86	50	1	4	88	198	283	3.04e-16	76.3
MS.gene062744.t1	MTR_1g021320	36.937	111	59	3	4	103	41	151	5.73e-16	75.5
MS.gene062744.t1	MTR_1g021290	34.532	139	71	4	4	130	171	301	3.77e-16	75.1
MS.gene062744.t1	MTR_1g021290	34.884	86	55	1	4	88	43	128	1.89e-11	62.0
MS.gene062744.t1	MTR_1g021440	28.758	153	102	2	4	149	38	190	7.49e-16	73.9
MS.gene062744.t1	MTR_1g021440	28.758	153	102	2	4	149	38	190	8.48e-16	73.9
MS.gene062744.t1	MTR_1g021440	28.758	153	102	2	4	149	38	190	1.68e-15	73.6
MS.gene062744.t1	MTR_1g021440	28.758	153	102	2	4	149	38	190	1.74e-15	73.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062744.t1	AT3G18990	36.441	118	63	3	3	112	21	134	7.01e-17	77.4
MS.gene062744.t1	AT3G18990	36.441	118	63	3	3	112	21	134	1.29e-16	76.6
MS.gene062744.t1	AT5G66980	24.828	145	102	3	4	142	25	168	8.60e-13	65.9
MS.gene062744.t1	AT4G01580	32.530	83	56	0	1	83	43	125	1.37e-11	60.8
MS.gene062744.t1	AT4G33280	35.238	105	60	3	4	100	39	143	2.70e-11	61.6
MS.gene062744.t1	AT3G06220	25.581	129	87	2	4	131	25	145	3.00e-11	59.7
MS.gene062744.t1	AT4G33280	35.238	105	60	3	4	100	39	143	3.59e-11	60.8

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Find 37 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCTATCCAATTATGAGT+TGG	0.532358	1.4:-3013496	MS.gene062744:CDS
AATCATCTGTTCTTCCATCA+TGG	0.499739	1.4:-3013807	MS.gene062744:CDS
ACTAGCTGAACATTCTTTGC+AGG	0.330880	1.4:+3013437	None:intergenic
ACTTGCCAAGTATTTCCACT+AGG	0.637449	1.4:+3013849	None:intergenic
ATCTGTTCTTCCATCATGGA+TGG	0.561421	1.4:-3013803	MS.gene062744:CDS
ATGCCGTGCCCTCCATTTGA+TGG	0.415676	1.4:+3013895	None:intergenic
ATTCTCACCAAATACTTGCA+CGG	0.664539	1.4:+3013704	None:intergenic
ATTTCTTCTTTGGATGTTGT+TGG	0.389381	1.4:-3013588	MS.gene062744:CDS
ATTTGATGGACAAAACCATC+TGG	0.494945	1.4:+3013909	None:intergenic
CAAATGGAGGGCACGGCATG+CGG	0.548985	1.4:-3013891	MS.gene062744:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAGAGGACGTAAATGATT+GGG	+	chr1.4:3013780-3013799	None:intergenic	30.0%
AGCAAATTCGATTTCATTCT+GGG	-	chr1.4:3013507-3013526	MS.gene062744:CDS	30.0%
ATTTCTTCTTTGGATGTTGT+TGG	-	chr1.4:3013283-3013302	MS.gene062744:intron	30.0%
GGAAATAGTAATGAAGTTGA+AGG	-	chr1.4:3013481-3013500	MS.gene062744:CDS	30.0%
GGAGATCATCATTTACAATA+TGG	-	chr1.4:3013103-3013122	MS.gene062744:intron	30.0%
TAGCAAATTCGATTTCATTC+TGG	-	chr1.4:3013506-3013525	MS.gene062744:CDS	30.0%
AATCATCTGTTCTTCCATCA+TGG	-	chr1.4:3013064-3013083	MS.gene062744:intron	35.0%
ATCAAAACCTTCAATGTCAG+TGG	-	chr1.4:3013818-3013837	MS.gene062744:CDS	35.0%
ATCTTCTTGAGAAACTGATC+TGG	-	chr1.4:3013907-3013926	MS.gene062744:CDS	35.0%
ATTCTCACCAAATACTTGCA+CGG	+	chr1.4:3013170-3013189	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	3012950	3013943	3012950	ID=MS.gene062744
chr1.4	mRNA	3012950	3013943	3012950	ID=MS.gene062744.t1;Parent=MS.gene062744
chr1.4	exon	3013391	3013943	3013391	ID=MS.gene062744.t1.exon1;Parent=MS.gene062744.t1
chr1.4	CDS	3013391	3013943	3013391	ID=cds.MS.gene062744.t1;Parent=MS.gene062744.t1
chr1.4	exon	3012950	3012954	3012950	ID=MS.gene062744.t1.exon2;Parent=MS.gene062744.t1
chr1.4	CDS	3012950	3012954	3012950	ID=cds.MS.gene062744.t1;Parent=MS.gene062744.t1

Gene Sequence

>MS.gene062744

ATGCAGAGAGTTCCAGATGGTTTTGTCCATCAAATGGAGGGCACGGCATGCGGATTAGTTTCATTAACTGGTCCTAGTGGAAATACTTGGCAAGTTCGATTAGTTGAGCAGGACAATCATCTGTTCTTCCATCATGGATGGTCAACATTTGTGGGAGATCATCATTTACAATATGGCGACCTTTTGGTTTTCAGATACGAGGGTCACTTGCATTTCACCGTGCAAGTATTTGGTGAGAATGCATGCGAGAAAGAAGCTGCATTTCATTCTGAGTGCAATCTAAATTCATTAAACTTCAATAATATTGAAGGACAGAAAAGTGATGCTGATGTGATTTCTTCTTTGGATGTTGTTGGAGATGTTCCGAAGAAAATGAGGTGCGACGCCATTGAGAACCAGGAGCCTGAGTTGGCTATTGCTGGTAAAAATCTATCCAATTATGAGTTGGTTAGACCAATCAGTATGATCAGAGAAACAGAAGAAACCTGCAAAGAATGTTCAGCTAGTGCTGTCCATGTTCCTTTTCAAACAGGAAATAGTAATGAAGTTGAAGGTATAGCAAATTCGATTTCATTCTGGGTCTCAAAAACATGTACTAAAACTGATACGATCTTCCCTAGAAAACTGATACTTGTCTAGTATAGAGTATTTTGCATTCAAATCCTTATATTTATGCCAGAGCTTATCATTTTATTTAAAACTCATGATTTAATTGTTGAATCTCACAGATGCAGCAATCCTGTATAGAAGTGGGAAGGAAGATGACCACGATATTCTCAGCGGAGCTTCACTATCAAAGTTATCAGCACATGATGAGAAAAAGATAGCCCAATCATTTACGTCCTCTTTTCCATATTTTGTTAAAATAATCAAAACCTTCAATGTCAGTGGTTCGTATACTCTGGTGAGTTGTTTCCTTTTGTCATTTATAATTTATCTCCCAAATAATGCTGTTTTATCTTCTTGAGAAACTGATCTGGTTGGGGCAGAGTAA

Protein sequence

>MS.gene062744.t1

MQRVPDGFVHQMEGTACGLVSLTGPSGNTWQVRLVEQDNHLFFHHGWSTFVGDHHLQYGDLLVFRYEGHLHFTVQVFGENACEKEAAFHSECNLNSLNFNNIEGQKSDADVISSLDVVGDVPKKMRCDAIENQEPELAIAGKNLSNYELVRPISMIRETEETCKECSASAVHVPFQTGNSNEVEE