AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene063006

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063006.t1 MTR_4g073240 100.000 148 0 0 1 148 1 148 4.12e-110 309
MS.gene063006.t1 MTR_3g062450 91.892 148 12 0 1 148 1 148 6.35e-103 291
MS.gene063006.t1 MTR_7g116940 92.568 148 11 0 1 148 1 148 9.75e-103 290
MS.gene063006.t1 MTR_5g075580 91.216 148 13 0 1 148 1 148 2.80e-102 289
MS.gene063006.t1 MTR_1g021150 88.514 148 17 0 1 148 1 148 6.90e-100 283
MS.gene063006.t1 MTR_7g116940 88.387 155 11 1 1 148 1 155 1.49e-99 283
MS.gene063006.t1 MTR_8g071040 87.838 148 18 0 1 148 1 148 1.44e-98 280
MS.gene063006.t1 MTR_4g073240 100.000 119 0 0 30 148 1 119 1.88e-87 251
MS.gene063006.t1 MTR_1g021150 88.889 126 14 0 23 148 31 156 1.59e-84 244
MS.gene063006.t1 MTR_5g075580 91.597 119 10 0 30 148 1 119 2.28e-81 235
MS.gene063006.t1 MTR_7g116940 90.826 109 10 0 1 109 1 109 2.77e-70 207
MS.gene063006.t1 MTR_4g073240 98.795 83 1 0 66 148 4 86 2.36e-57 173
MS.gene063006.t1 MTR_8g085690 51.724 145 70 0 2 146 23 167 1.10e-54 169
MS.gene063006.t1 MTR_2g078010 46.897 145 77 0 4 148 8 152 1.06e-45 146
MS.gene063006.t1 MTR_8g468530 45.205 146 75 1 2 142 21 166 3.65e-44 143
MS.gene063006.t1 MTR_4g108080 44.521 146 76 1 2 142 5 150 2.18e-43 140
MS.gene063006.t1 MTR_2g078010 42.767 159 77 1 4 148 8 166 1.23e-41 136
MS.gene063006.t1 MTR_2g078010 46.715 137 73 0 4 140 8 144 3.37e-41 135
MS.gene063006.t1 MTR_3g098780 48.611 144 72 2 5 146 6 149 2.56e-40 134
MS.gene063006.t1 MTR_3g098780 48.611 144 72 2 5 146 30 173 6.74e-40 134
MS.gene063006.t1 MTR_3g110110 41.497 147 82 3 5 147 7 153 1.53e-34 118
MS.gene063006.t1 MTR_4g076200 44.715 123 68 0 4 126 35 157 4.81e-34 117
MS.gene063006.t1 MTR_1g095510 47.200 125 65 1 3 126 43 167 6.76e-34 117
MS.gene063006.t1 MTR_4g108080 46.667 105 56 0 2 106 5 109 7.05e-34 115
MS.gene063006.t1 MTR_1g095510 50.476 105 52 0 22 126 9 113 1.04e-32 113
MS.gene063006.t1 MTR_5g009020 43.089 123 70 0 4 126 35 157 3.05e-32 113
MS.gene063006.t1 MTR_4g076200 44.737 114 63 0 13 126 3 116 3.92e-32 111
MS.gene063006.t1 MTR_1g095510 49.550 111 55 1 3 112 43 153 1.49e-31 111
MS.gene063006.t1 MTR_2g078010 48.515 101 52 0 4 104 8 108 3.68e-31 108
MS.gene063006.t1 MTR_4g108080 45.872 109 54 1 39 142 42 150 3.42e-30 107
MS.gene063006.t1 MTR_5g019480 38.971 136 80 2 5 139 33 166 1.88e-28 103
MS.gene063006.t1 MTR_5g019480 38.971 136 80 2 5 139 36 169 2.00e-28 103
MS.gene063006.t1 MTR_4g094872 34.868 152 92 2 1 145 4 155 1.65e-27 100
MS.gene063006.t1 MTR_5g019480 40.870 115 68 0 25 139 4 118 5.06e-27 98.6
MS.gene063006.t1 MTR_4g063490 35.821 134 72 2 15 134 18 151 1.51e-26 98.2
MS.gene063006.t1 MTR_8g028920 35.772 123 77 2 26 146 25 147 1.30e-25 96.3
MS.gene063006.t1 MTR_5g016630 32.895 152 95 2 1 145 4 155 1.39e-25 95.5
MS.gene063006.t1 MTR_8g098595 37.273 110 67 2 39 146 23 132 2.08e-24 92.8
MS.gene063006.t1 MTR_2g096690 34.028 144 81 2 15 144 18 161 5.41e-24 91.7
MS.gene063006.t1 MTR_7g087590 34.532 139 91 0 4 142 13 151 5.92e-24 94.0
MS.gene063006.t1 MTR_2g096690 37.600 125 64 2 15 125 18 142 1.15e-23 90.9
MS.gene063006.t1 MTR_8g028920 37.736 106 64 2 43 146 3 108 7.37e-23 88.2
MS.gene063006.t1 MTR_4g106690 29.268 123 85 2 26 146 25 147 4.13e-21 84.7
MS.gene063006.t1 MTR_8g076490 39.806 103 60 2 1 102 15 116 3.70e-19 79.0
MS.gene063006.t1 MTR_4g121960 33.613 119 69 4 2 114 11 125 2.18e-17 77.0
MS.gene063006.t1 MTR_5g021260 36.893 103 63 2 1 102 15 116 3.12e-17 73.9
MS.gene063006.t1 MTR_2g096690 37.374 99 48 2 15 99 18 116 2.08e-16 70.9
MS.gene063006.t1 MTR_7g451800 35.246 122 69 3 4 115 38 159 1.06e-15 72.8
MS.gene063006.t1 MTR_7g047620 35.246 122 69 3 4 115 38 159 1.06e-15 72.8
MS.gene063006.t1 MTR_8g023380 31.624 117 71 3 4 115 8 120 1.71e-15 71.2
MS.gene063006.t1 MTR_7g047570 35.246 122 69 3 4 115 38 159 2.50e-15 71.6
MS.gene063006.t1 MTR_7g451770 35.246 122 69 3 4 115 38 159 2.50e-15 71.6
MS.gene063006.t1 MTR_0274s0030 32.520 123 73 3 3 115 113 235 2.43e-14 68.9
MS.gene063006.t1 MTR_7g023760 30.894 123 75 3 3 115 90 212 2.48e-14 68.6
MS.gene063006.t1 MTR_4g088835 30.000 150 92 4 4 143 672 818 3.73e-14 68.9
MS.gene063006.t1 MTR_4g020620 31.707 123 74 3 3 115 628 750 2.51e-13 66.6
MS.gene063006.t1 MTR_4g020620 31.707 123 74 3 3 115 570 692 3.13e-13 66.2
MS.gene063006.t1 MTR_2g013650 27.815 151 96 4 3 143 681 828 3.34e-13 66.2
MS.gene063006.t1 MTR_4g086440 31.967 122 73 3 4 115 37 158 7.68e-13 64.7
MS.gene063006.t1 MTR_1g028540 67.391 46 15 0 102 147 83 128 9.72e-13 61.6
MS.gene063006.t1 MTR_1g092460 29.268 123 77 3 3 115 176 298 3.30e-12 63.2
MS.gene063006.t1 MTR_2g079270 30.894 123 75 3 3 115 306 428 3.80e-12 63.2
MS.gene063006.t1 MTR_1g073600 25.688 109 67 2 33 140 25 120 5.30e-12 59.7
MS.gene063006.t1 MTR_4g007210 30.216 139 86 4 3 130 80 218 1.03e-11 61.6
MS.gene063006.t1 MTR_1g092440 34.783 92 50 3 34 115 411 502 1.21e-11 61.6
MS.gene063006.t1 MTR_1g092465 29.221 154 79 6 3 144 241 376 1.94e-11 60.8
MS.gene063006.t1 MTR_2g018540 32.000 125 73 3 3 115 107 231 3.17e-11 60.1
MS.gene063006.t1 MTR_1g112730 30.328 122 75 3 4 115 856 977 5.55e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063006.t1 AT1G64230 91.892 148 12 0 1 148 1 148 1.84e-103 292
MS.gene063006.t1 AT1G64230 91.892 148 12 0 1 148 1 148 1.84e-103 292
MS.gene063006.t1 AT5G53300 91.892 148 12 0 1 148 1 148 1.40e-102 290
MS.gene063006.t1 AT5G53300 91.892 148 12 0 1 148 1 148 1.40e-102 290
MS.gene063006.t1 AT5G53300 91.892 148 12 0 1 148 1 148 1.40e-102 290
MS.gene063006.t1 AT4G27960 91.216 148 13 0 1 148 1 148 3.34e-102 289
MS.gene063006.t1 AT5G41700 90.541 148 14 0 1 148 1 148 7.44e-102 288
MS.gene063006.t1 AT5G41700 90.541 148 14 0 1 148 1 148 7.44e-102 288
MS.gene063006.t1 AT5G41700 90.541 148 14 0 1 148 1 148 7.44e-102 288
MS.gene063006.t1 AT4G27960 91.216 148 13 0 1 148 31 178 2.20e-101 288
MS.gene063006.t1 AT3G08690 88.514 148 17 0 1 148 1 148 8.16e-100 283
MS.gene063006.t1 AT3G08690 88.514 148 17 0 1 148 1 148 8.16e-100 283
MS.gene063006.t1 AT5G56150 88.514 148 17 0 1 148 1 148 4.67e-99 281
MS.gene063006.t1 AT5G41700 89.262 149 15 1 1 148 1 149 5.95e-99 281
MS.gene063006.t1 AT1G64230 85.987 157 13 1 1 148 1 157 1.01e-98 281
MS.gene063006.t1 AT2G16740 85.714 147 21 0 1 147 1 147 3.23e-97 276
MS.gene063006.t1 AT1G64230 71.053 190 13 1 1 148 1 190 5.96e-94 270
MS.gene063006.t1 AT1G64230 71.053 190 13 1 1 148 1 190 5.96e-94 270
MS.gene063006.t1 AT3G08700 75.839 149 35 1 1 148 1 149 5.25e-84 243
MS.gene063006.t1 AT3G08700 75.839 149 35 1 1 148 1 149 5.25e-84 243
MS.gene063006.t1 AT5G41700 91.346 104 9 0 1 104 1 104 1.95e-69 206
MS.gene063006.t1 AT5G53300 89.908 109 9 1 1 107 1 109 2.11e-69 205
MS.gene063006.t1 AT5G56150 87.379 103 13 0 1 103 1 103 1.02e-65 196
MS.gene063006.t1 AT3G13550 48.966 145 74 0 2 146 37 181 1.45e-53 167
MS.gene063006.t1 AT1G16890 48.276 145 75 0 4 148 8 152 5.39e-47 149
MS.gene063006.t1 AT1G78870 47.586 145 76 0 4 148 8 152 3.56e-46 147
MS.gene063006.t1 AT5G62540 48.872 133 68 0 2 134 5 137 1.91e-44 143
MS.gene063006.t1 AT1G16890 46.104 154 74 1 4 148 8 161 2.87e-44 143
MS.gene063006.t1 AT2G02760 44.521 146 76 1 2 142 5 150 1.63e-43 140
MS.gene063006.t1 AT2G02760 44.521 146 76 1 2 142 5 150 1.63e-43 140
MS.gene063006.t1 AT2G02760 44.521 146 76 1 2 142 5 150 1.63e-43 140
MS.gene063006.t1 AT2G02760 44.521 146 76 1 2 142 5 150 1.63e-43 140
MS.gene063006.t1 AT1G14400 44.521 146 76 1 2 142 5 150 1.74e-43 140
MS.gene063006.t1 AT1G14400 44.521 146 76 1 2 142 5 150 1.74e-43 140
MS.gene063006.t1 AT3G13550 51.754 114 55 0 2 115 37 150 1.87e-43 141
MS.gene063006.t1 AT1G36340 46.897 145 76 1 2 146 9 152 4.48e-43 140
MS.gene063006.t1 AT1G36340 46.897 145 76 1 2 146 9 152 4.48e-43 140
MS.gene063006.t1 AT1G16890 50.420 119 59 0 30 148 1 119 5.77e-40 130
MS.gene063006.t1 AT1G78870 50.420 119 59 0 30 148 1 119 9.75e-40 130
MS.gene063006.t1 AT5G50870 47.917 144 73 2 5 146 6 149 2.02e-38 129
MS.gene063006.t1 AT1G78870 50.000 120 59 1 30 148 1 120 5.77e-38 125
MS.gene063006.t1 AT5G50870 44.516 155 73 3 5 146 6 160 1.80e-35 122
MS.gene063006.t1 AT3G24515 43.949 157 75 3 3 146 8 164 1.98e-33 121
MS.gene063006.t1 AT5G25760 40.136 147 84 3 5 147 7 153 4.26e-33 114
MS.gene063006.t1 AT5G25760 40.136 147 84 3 5 147 7 153 4.26e-33 114
MS.gene063006.t1 AT2G32790 51.587 126 57 2 25 148 54 177 9.20e-33 114
MS.gene063006.t1 AT1G78870 48.571 105 54 0 4 108 8 112 2.75e-32 111
MS.gene063006.t1 AT3G20060 43.089 123 70 0 4 126 39 161 7.62e-31 109
MS.gene063006.t1 AT3G24515 45.902 122 62 1 29 146 68 189 1.71e-30 114
MS.gene063006.t1 AT1G50490 43.902 123 69 0 4 126 38 160 2.26e-30 108
MS.gene063006.t1 AT3G20060 45.714 105 57 0 22 126 12 116 3.86e-29 103
MS.gene063006.t1 AT3G57870 35.526 152 91 3 1 145 5 156 1.84e-27 100
MS.gene063006.t1 AT4G36800 38.235 136 81 2 5 139 34 167 3.12e-27 100
MS.gene063006.t1 AT4G36800 38.235 136 81 2 5 139 34 167 3.12e-27 100
MS.gene063006.t1 AT2G46030 35.135 148 93 3 1 146 1 147 3.66e-27 100
MS.gene063006.t1 AT2G46030 35.135 148 93 3 1 146 1 147 3.66e-27 100
MS.gene063006.t1 AT5G05080 37.410 139 87 0 4 142 13 151 5.70e-27 101
MS.gene063006.t1 AT5G05080 37.410 139 87 0 4 142 13 151 5.70e-27 101
MS.gene063006.t1 AT5G05080 37.410 139 87 0 4 142 13 151 5.70e-27 101
MS.gene063006.t1 AT5G05080 37.410 139 87 0 4 142 13 151 5.70e-27 101
MS.gene063006.t1 AT2G46030 35.135 148 93 3 1 146 73 219 9.78e-27 100
MS.gene063006.t1 AT2G46030 38.211 123 74 2 26 146 28 150 9.95e-27 99.4
MS.gene063006.t1 AT2G46030 38.211 123 74 2 26 146 28 150 9.95e-27 99.4
MS.gene063006.t1 AT5G41340 36.585 123 76 2 26 146 25 147 6.78e-26 97.1
MS.gene063006.t1 AT1G63800 36.585 123 76 2 26 146 10 132 8.49e-26 96.3
MS.gene063006.t1 AT1G63800 36.585 123 76 2 26 146 25 147 9.21e-26 96.7
MS.gene063006.t1 AT3G55380 35.075 134 73 2 15 134 19 152 2.55e-25 95.1
MS.gene063006.t1 AT5G59300 36.296 135 72 2 15 135 18 152 1.05e-24 93.6
MS.gene063006.t1 AT3G46460 34.028 144 81 2 15 144 18 161 1.57e-24 93.2
MS.gene063006.t1 AT2G18600 36.496 137 82 4 5 139 35 168 3.03e-24 92.8
MS.gene063006.t1 AT2G18600 36.496 137 82 4 5 139 35 168 3.03e-24 92.8
MS.gene063006.t1 AT5G41340 38.462 104 62 2 45 146 9 112 9.90e-23 88.2
MS.gene063006.t1 AT3G17000 36.134 119 66 4 2 114 12 126 3.13e-19 82.0
MS.gene063006.t1 AT1G75440 38.835 103 61 2 1 102 15 116 6.08e-19 78.6
MS.gene063006.t1 AT3G55380 35.965 114 57 3 15 112 19 132 3.29e-18 77.4
MS.gene063006.t1 AT1G45050 37.864 103 62 2 1 102 15 116 4.78e-18 76.3
MS.gene063006.t1 AT5G42990 38.835 103 61 2 1 102 15 116 9.67e-18 75.5
MS.gene063006.t1 AT1G17280 33.333 117 69 3 4 115 8 120 9.98e-17 74.3
MS.gene063006.t1 AT1G17280 33.333 117 69 3 4 115 8 120 9.98e-17 74.3
MS.gene063006.t1 AT1G17280 33.333 117 69 3 4 115 8 120 9.98e-17 74.3
MS.gene063006.t1 AT1G17280 33.333 117 69 3 4 115 8 120 9.98e-17 74.3
MS.gene063006.t1 AT1G17280 33.333 117 69 3 4 115 8 120 9.98e-17 74.3
MS.gene063006.t1 AT1G17280 33.333 117 69 3 4 115 8 120 9.98e-17 74.3
MS.gene063006.t1 AT1G17280 33.333 117 69 3 4 115 8 120 9.98e-17 74.3
MS.gene063006.t1 AT1G17280 33.333 117 69 3 4 115 8 120 9.98e-17 74.3
MS.gene063006.t1 AT4G36410 35.185 108 64 3 1 107 15 117 1.30e-16 72.4
MS.gene063006.t1 AT5G50430 33.333 117 69 3 4 115 8 120 1.31e-16 74.3
MS.gene063006.t1 AT5G50430 33.333 117 69 3 4 115 8 120 1.31e-16 74.3
MS.gene063006.t1 AT3G46460 30.631 111 64 1 47 144 9 119 4.22e-16 70.1
MS.gene063006.t1 AT3G46460 30.631 111 64 1 47 144 9 119 4.22e-16 70.1
MS.gene063006.t1 AT1G17280 30.894 123 70 3 4 115 8 126 1.90e-15 70.9
MS.gene063006.t1 AT3G15355 29.139 151 94 4 3 143 334 481 7.03e-12 62.4
MS.gene063006.t1 AT1G53025 31.148 122 74 3 4 115 274 395 2.00e-11 60.8
MS.gene063006.t1 AT1G45050 34.831 89 56 2 1 88 15 102 2.04e-11 57.8
MS.gene063006.t1 AT1G53025 31.148 122 74 3 4 115 274 395 2.09e-11 60.8
MS.gene063006.t1 AT1G53023 31.148 122 74 3 4 115 60 181 9.10e-11 58.9

Find 48 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGGCAAGCAACCATCAT+TGG 0.350944 4.2:+35206133 MS.gene063006:CDS
GAGAATAGTTAAGGAGCTTA+AGG 0.366328 4.2:+35205973 MS.gene063006:CDS
GGAGGTTTGAAGGGATAATC+AGG 0.402929 4.2:-35206211 None:intergenic
ACCTTGGATATTGTGAGAGC+AGG 0.413179 4.2:-35206943 None:intergenic
ACAAGAGGGTCATCAGGATT+TGG 0.424690 4.2:-35207093 None:intergenic
ATCCGTTAGCAGAGAACATA+TGG 0.433316 4.2:-35207070 None:intergenic
CCAGCATAGGGACTATCATT+TGG 0.439200 4.2:-35206157 None:intergenic
AAGCTCCTTAACTATTCTCT+TGG 0.446675 4.2:-35205969 None:intergenic
GTATACCTTGGGAGGTTTGA+AGG 0.451790 4.2:-35206221 None:intergenic
TCGGCGAGGACATGTTTCAT+TGG 0.472144 4.2:+35206116 MS.gene063006:CDS
ATTGTTCTTTGAGTATGTCT+AGG 0.473064 4.2:-35206915 None:intergenic
TCAGGCACAAGAGGGTCATC+AGG 0.475511 4.2:-35207099 None:intergenic
ACTATCATTTGGACCAATGA+TGG 0.481239 4.2:-35206146 None:intergenic
TGAAACATGTCCTCGCCGAC+TGG 0.490214 4.2:-35206112 None:intergenic
TTGCACATATGAGCAACTTC+AGG 0.491907 4.2:-35207117 None:intergenic
CTCCATATGTTCTCTGCTAA+CGG 0.494453 4.2:+35207068 MS.gene063006:CDS
TTGCCATTGCTGTTAATGTT+GGG 0.496480 4.2:-35206886 None:intergenic
CCTGCACTGCATGAGGTTGG+TGG 0.496861 4.2:-35206010 None:intergenic
CCACCAACCTCATGCAGTGC+AGG 0.500851 4.2:+35206010 MS.gene063006:CDS
CTGGACTCAGAAATTTGCTA+TGG 0.506602 4.2:+35207176 MS.gene063006:CDS
AAGGAAAACACCACCAGCAT+AGG 0.515996 4.2:-35206170 None:intergenic
ATTGCCATTGCTGTTAATGT+TGG 0.518283 4.2:-35206887 None:intergenic
ATGAGTCCACTGCTAGAAGC+TGG 0.523736 4.2:+35207157 MS.gene063006:CDS
TTACCTGCACTGCATGAGGT+TGG 0.530132 4.2:-35206013 None:intergenic
GGCGTCCAAGAGAATAGTTA+AGG 0.531160 4.2:+35205964 MS.gene063006:CDS
TATACCTTGGGAGGTTTGAA+GGG 0.541616 4.2:-35206220 None:intergenic
CTCTTTACCTGCACTGCATG+AGG 0.548184 4.2:-35206017 None:intergenic
CCTTGGATATTGTGAGAGCA+GGG 0.575241 4.2:-35206942 None:intergenic
CTGAGTCCAGCTTCTAGCAG+TGG 0.582567 4.2:-35207163 None:intergenic
AGGAAAACACCACCAGCATA+GGG 0.585785 4.2:-35206169 None:intergenic
CCAAATGATAGTCCCTATGC+TGG 0.590321 4.2:+35206157 MS.gene063006:CDS
ACTTTGGTGTATACCTTGGG+AGG 0.590340 4.2:-35206229 None:intergenic
TTATCCCTTCAAACCTCCCA+AGG 0.591046 4.2:+35206216 MS.gene063006:CDS
ATAATCAGGAGGAAAATGAA+TGG 0.593685 4.2:-35206197 None:intergenic
CATTGCTGTTAATGTTGGGA+TGG 0.595351 4.2:-35206882 None:intergenic
TGGACTCAGAAATTTGCTAT+GGG 0.595611 4.2:+35207177 MS.gene063006:CDS
CCCTGCTCTCACAATATCCA+AGG 0.597960 4.2:+35206942 MS.gene063006:CDS
TGAGCAACTTCAGGCACAAG+AGG 0.601737 4.2:-35207108 None:intergenic
CATCCCAACATTAACAGCAA+TGG 0.604832 4.2:+35206883 MS.gene063006:CDS
TGATGAGTTTCAGGTCCAGT+CGG 0.607354 4.2:+35206097 MS.gene063006:intron
GCAGGTTGCATTCAGGACCA+AGG 0.630304 4.2:+35206855 MS.gene063006:intron
TAGACATACTCAAAGAACAA+TGG 0.637759 4.2:+35206917 MS.gene063006:CDS
GAGCAACTTCAGGCACAAGA+GGG 0.655538 4.2:-35207107 None:intergenic
GAGGAAAATGAATGGTGACA+AGG 0.659276 4.2:-35206189 None:intergenic
AGTTTCAGGTCCAGTCGGCG+AGG 0.671300 4.2:+35206102 MS.gene063006:intron
AATGTTGGGATGGAATACCT+TGG 0.674072 4.2:-35206872 None:intergenic
AATGATAGTCCCTATGCTGG+TGG 0.684815 4.2:+35206160 MS.gene063006:CDS
GGTTTGAAGGGATAATCAGG+AGG 0.711960 4.2:-35206208 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAATAAACCTATTATA+TGG - chr4.2:35206638-35206657 None:intergenic 10.0%
!! ATATTATATGATTGTATAAT+AGG + chr4.2:35206390-35206409 MS.gene063006:intron 10.0%
!! TATTATATGATTGTATAATA+GGG + chr4.2:35206391-35206410 MS.gene063006:intron 10.0%
!!! AATATCAAGATTTTAAATCT+TGG - chr4.2:35206800-35206819 None:intergenic 15.0%
!!! TTCAGCATTTTTATATAAAA+CGG - chr4.2:35207013-35207032 None:intergenic 15.0%
!! AAGACAAACATATCTATTTA+AGG - chr4.2:35206361-35206380 None:intergenic 20.0%
!!! AGTATAAAAAATGATTTTGC+AGG + chr4.2:35206837-35206856 MS.gene063006:intron 20.0%
! AAAAAGATCTGTTGTGATTT+AGG + chr4.2:35206661-35206680 MS.gene063006:intron 25.0%
! AATAAACCTATTATATGGAC+TGG - chr4.2:35206633-35206652 None:intergenic 25.0%
! CTGGAATAAATAAAGAAAAC+AGG - chr4.2:35206275-35206294 None:intergenic 25.0%
! GTTAAAAATCATGATGATAC+AGG + chr4.2:35207038-35207057 MS.gene063006:intron 25.0%
!!! TGTTTTTTATGATGAGTTTC+AGG + chr4.2:35206088-35206107 MS.gene063006:intron 25.0%
ACCATCTAAACTCACAATAA+CGG + chr4.2:35206445-35206464 MS.gene063006:intron 30.0%
AGTTTGAATGAAACTTGAAC+TGG + chr4.2:35206038-35206057 MS.gene063006:intron 30.0%
ATAATCAGGAGGAAAATGAA+TGG - chr4.2:35206200-35206219 None:intergenic 30.0%
ATTGTTCTTTGAGTATGTCT+AGG - chr4.2:35206918-35206937 None:intergenic 30.0%
GTTAATAAGTGACTCTAGAT+AGG + chr4.2:35206304-35206323 MS.gene063006:intron 30.0%
TAGACATACTCAAAGAACAA+TGG + chr4.2:35206917-35206936 MS.gene063006:CDS 30.0%
TGCAACTATCTGTAATATCA+AGG + chr4.2:35206759-35206778 MS.gene063006:intron 30.0%
! ATCAGTCGTTAATTTGAGAT+CGG - chr4.2:35206517-35206536 None:intergenic 30.0%
! GTATAATAGGGATTTGAGTT+TGG + chr4.2:35206403-35206422 MS.gene063006:intron 30.0%
!!! ACAGTGTTTTAAAAGCACTT+TGG - chr4.2:35206248-35206267 None:intergenic 30.0%
AAGCTCCTTAACTATTCTCT+TGG - chr4.2:35205972-35205991 None:intergenic 35.0%
ACCGTTATTGTGAGTTTAGA+TGG - chr4.2:35206449-35206468 None:intergenic 35.0%
ACTATCATTTGGACCAATGA+TGG - chr4.2:35206149-35206168 None:intergenic 35.0%
ACTTATTAACAAGATGCAGC+TGG - chr4.2:35206294-35206313 None:intergenic 35.0%
AGTTACGGTTACAGATACAT+CGG - chr4.2:35206704-35206723 None:intergenic 35.0%
GAGAATAGTTAAGGAGCTTA+AGG + chr4.2:35205973-35205992 MS.gene063006:CDS 35.0%
GTTATTGTGAGTTTAGATGG+TGG - chr4.2:35206446-35206465 None:intergenic 35.0%
TAAGGCTATCACACACAAAA+AGG - chr4.2:35206343-35206362 None:intergenic 35.0%
TTTAGGCCAGTCCATATAAT+AGG + chr4.2:35206624-35206643 MS.gene063006:intron 35.0%
! ATTGCCATTGCTGTTAATGT+TGG - chr4.2:35206890-35206909 None:intergenic 35.0%
! TCAGTCGTTAATTTGAGATC+GGG - chr4.2:35206516-35206535 None:intergenic 35.0%
! TTGCCATTGCTGTTAATGTT+GGG - chr4.2:35206889-35206908 None:intergenic 35.0%
!! AAGGTTTGCTGTTGCAATTT+AGG + chr4.2:35206607-35206626 MS.gene063006:intron 35.0%
!! TGGACTCAGAAATTTGCTAT+GGG + chr4.2:35207177-35207196 MS.gene063006:CDS 35.0%
AATGTTGGGATGGAATACCT+TGG - chr4.2:35206875-35206894 None:intergenic 40.0%
AGAAACTAATGACGTCAACG+AGG - chr4.2:35206988-35207007 None:intergenic 40.0%
ATCCGTTAGCAGAGAACATA+TGG - chr4.2:35207073-35207092 None:intergenic 40.0%
CATCCCAACATTAACAGCAA+TGG + chr4.2:35206883-35206902 MS.gene063006:CDS 40.0%
CATCGGAAATCTTGATGTCA+AGG - chr4.2:35206687-35206706 None:intergenic 40.0%
CTCCATATGTTCTCTGCTAA+CGG + chr4.2:35207068-35207087 MS.gene063006:CDS 40.0%
GAGGAAAATGAATGGTGACA+AGG - chr4.2:35206192-35206211 None:intergenic 40.0%
GGTTCACAATAACATGACCT+TGG - chr4.2:35206962-35206981 None:intergenic 40.0%
TATACCTTGGGAGGTTTGAA+GGG - chr4.2:35206223-35206242 None:intergenic 40.0%
TTGCACATATGAGCAACTTC+AGG - chr4.2:35207120-35207139 None:intergenic 40.0%
! AAGCACTTTGGTGTATACCT+TGG - chr4.2:35206236-35206255 None:intergenic 40.0%
! AGCACTTTGGTGTATACCTT+GGG - chr4.2:35206235-35206254 None:intergenic 40.0%
! CATTGCTGTTAATGTTGGGA+TGG - chr4.2:35206885-35206904 None:intergenic 40.0%
! TAGGGATTTGAGTTTGGACA+CGG + chr4.2:35206409-35206428 MS.gene063006:intron 40.0%
! TGATTTTGCAGGTTGCATTC+AGG + chr4.2:35206848-35206867 MS.gene063006:intron 40.0%
!! CTGGACTCAGAAATTTGCTA+TGG + chr4.2:35207176-35207195 MS.gene063006:CDS 40.0%
AAGGAAAACACCACCAGCAT+AGG - chr4.2:35206173-35206192 None:intergenic 45.0%
AATGATAGTCCCTATGCTGG+TGG + chr4.2:35206160-35206179 MS.gene063006:CDS 45.0%
ACAAGAGGGTCATCAGGATT+TGG - chr4.2:35207096-35207115 None:intergenic 45.0%
ACCTTGGATATTGTGAGAGC+AGG - chr4.2:35206946-35206965 None:intergenic 45.0%
AGGAAAACACCACCAGCATA+GGG - chr4.2:35206172-35206191 None:intergenic 45.0%
CATTGGCAAGCAACCATCAT+TGG + chr4.2:35206133-35206152 MS.gene063006:CDS 45.0%
CCAAATGATAGTCCCTATGC+TGG + chr4.2:35206157-35206176 MS.gene063006:CDS 45.0%
CCAGCATAGGGACTATCATT+TGG - chr4.2:35206160-35206179 None:intergenic 45.0%
CCTTGGATATTGTGAGAGCA+GGG - chr4.2:35206945-35206964 None:intergenic 45.0%
CTAATGACGTCAACGAGGAA+TGG - chr4.2:35206983-35207002 None:intergenic 45.0%
CTGTAACCGTAACTTGACAG+CGG + chr4.2:35206710-35206729 MS.gene063006:intron 45.0%
GGAGGTTTGAAGGGATAATC+AGG - chr4.2:35206214-35206233 None:intergenic 45.0%
GGCGTCCAAGAGAATAGTTA+AGG + chr4.2:35205964-35205983 MS.gene063006:CDS 45.0%
GGTTTGAAGGGATAATCAGG+AGG - chr4.2:35206211-35206230 None:intergenic 45.0%
GTATACCTTGGGAGGTTTGA+AGG - chr4.2:35206224-35206243 None:intergenic 45.0%
TTATCCCTTCAAACCTCCCA+AGG + chr4.2:35206216-35206235 MS.gene063006:CDS 45.0%
! ACTTTGGTGTATACCTTGGG+AGG - chr4.2:35206232-35206251 None:intergenic 45.0%
! AGGGATTTGAGTTTGGACAC+GGG + chr4.2:35206410-35206429 MS.gene063006:intron 45.0%
! GTCGTTAATTTGAGATCGGG+CGG - chr4.2:35206513-35206532 None:intergenic 45.0%
! TGATGAGTTTCAGGTCCAGT+CGG + chr4.2:35206097-35206116 MS.gene063006:intron 45.0%
!! TCTTAAAAAAAAAAAAAAAA+AGG - chr4.2:35206585-35206604 None:intergenic 5.0%
!!! TTTTTTTTTTTTTAAGAAAA+AGG + chr4.2:35206588-35206607 MS.gene063006:intron 5.0%
ATGAGTCCACTGCTAGAAGC+TGG + chr4.2:35207157-35207176 MS.gene063006:CDS 50.0%
CCCTGCTCTCACAATATCCA+AGG + chr4.2:35206942-35206961 MS.gene063006:CDS 50.0%
CCTCAACCGCTGTCAAGTTA+CGG - chr4.2:35206719-35206738 None:intergenic 50.0%
CTCTTTACCTGCACTGCATG+AGG - chr4.2:35206020-35206039 None:intergenic 50.0%
GAGCAACTTCAGGCACAAGA+GGG - chr4.2:35207110-35207129 None:intergenic 50.0%
TCGGCGAGGACATGTTTCAT+TGG + chr4.2:35206116-35206135 MS.gene063006:CDS 50.0%
TGAGCAACTTCAGGCACAAG+AGG - chr4.2:35207111-35207130 None:intergenic 50.0%
TTACCTGCACTGCATGAGGT+TGG - chr4.2:35206016-35206035 None:intergenic 50.0%
CCGTAACTTGACAGCGGTTG+AGG + chr4.2:35206716-35206735 MS.gene063006:intron 55.0%
CTGAGTCCAGCTTCTAGCAG+TGG - chr4.2:35207166-35207185 None:intergenic 55.0%
GCAGGTTGCATTCAGGACCA+AGG + chr4.2:35206855-35206874 MS.gene063006:intron 55.0%
TCAGGCACAAGAGGGTCATC+AGG - chr4.2:35207102-35207121 None:intergenic 55.0%
TGAAACATGTCCTCGCCGAC+TGG - chr4.2:35206115-35206134 None:intergenic 55.0%
CCACCAACCTCATGCAGTGC+AGG + chr4.2:35206010-35206029 MS.gene063006:CDS 60.0%
CCTGCACTGCATGAGGTTGG+TGG - chr4.2:35206013-35206032 None:intergenic 60.0%
! AGTTTCAGGTCCAGTCGGCG+AGG + chr4.2:35206102-35206121 MS.gene063006:intron 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 35205962 35207203 35205962 ID=MS.gene063006
chr4.2 mRNA 35205962 35207203 35205962 ID=MS.gene063006.t1;Parent=MS.gene063006
chr4.2 exon 35205962 35206031 35205962 ID=MS.gene063006.t1.exon1;Parent=MS.gene063006.t1
chr4.2 CDS 35205962 35206031 35205962 ID=cds.MS.gene063006.t1;Parent=MS.gene063006.t1
chr4.2 exon 35206110 35206237 35206110 ID=MS.gene063006.t1.exon2;Parent=MS.gene063006.t1
chr4.2 CDS 35206110 35206237 35206110 ID=cds.MS.gene063006.t1;Parent=MS.gene063006.t1
chr4.2 exon 35206859 35206963 35206859 ID=MS.gene063006.t1.exon3;Parent=MS.gene063006.t1
chr4.2 CDS 35206859 35206963 35206859 ID=cds.MS.gene063006.t1;Parent=MS.gene063006.t1
chr4.2 exon 35207060 35207203 35207060 ID=MS.gene063006.t1.exon4;Parent=MS.gene063006.t1
chr4.2 CDS 35207060 35207203 35207060 ID=cds.MS.gene063006.t1;Parent=MS.gene063006.t1
Gene Sequence

>MS.gene063006

ATGGCGTCCAAGAGAATAGTTAAGGAGCTTAAGGACTTGCAGAGAGATCCACCAACCTCATGCAGTGCAGGTAAAGAGTTTGAATGAAACTTGAACTGGTTTATTTAACAAAACTGTAGTGTGATGTGTTTTTTATGATGAGTTTCAGGTCCAGTCGGCGAGGACATGTTTCATTGGCAAGCAACCATCATTGGTCCAAATGATAGTCCCTATGCTGGTGGTGTTTTCCTTGTCACCATTCATTTTCCTCCTGATTATCCCTTCAAACCTCCCAAGGTATACACCAAAGTGCTTTTAAAACACTGTGATACCTGTTTTCTTTATTTATTCCAGCTGCATCTTGTTAATAAGTGACTCTAGATAGGCAAAAAGTCTCTTCCTTTTTGTGTGTGATAGCCTTAAATAGATATGTTTGTCTTCAACTTTATATATTATATGATTGTATAATAGGGATTTGAGTTTGGACACGGGTTTTCAATCTCCACCATCTAAACTCACAATAACGGTCAACGTCGTTTTGAACTGATTTTGTTATTGTGTAATCTGAACCGCCCGATCTCAAATTAACGACTGATATAGAAAACTGCATGTAACTGTGTCATTTTTTTACTGCAGTAGATCCTTTTTTTTTTTTTTTTTAAGAAAAAGGTTTGCTGTTGCAATTTAGGCCAGTCCATATAATAGGTTTATTTTTTTAAGAAAAAGATCTGTTGTGATTTAGGCCTTGACATCAAGATTTCCGATGTATCTGTAACCGTAACTTGACAGCGGTTGAGGCTGCATCAGCTGTATTTGACTGCAACTATCTGTAATATCAAGGATTGATGCTACATAACCAAGATTTAAAATCTTGATATTCAATATAATTGATGACTAGTATAAAAAATGATTTTGCAGGTTGCATTCAGGACCAAGGTATTCCATCCCAACATTAACAGCAATGGCAATATTTGCCTAGACATACTCAAAGAACAATGGAGCCCTGCTCTCACAATATCCAAGGTCATGTTATTGTGAACCATTCCTCGTTGACGTCATTAGTTTCTATCCGTTTTATATAAAAATGCTGAATGATTGTTAAAAATCATGATGATACAGGTACTGCTCTCCATATGTTCTCTGCTAACGGATCCAAATCCTGATGACCCTCTTGTGCCTGAAGTTGCTCATATGTGCAAGACAGACAGAGTCAAGTATGAGTCCACTGCTAGAAGCTGGACTCAGAAATTTGCTATGGGCTAA
Protein sequence

>MS.gene063006.t1

MASKRIVKELKDLQRDPPTSCSAGPVGEDMFHWQATIIGPNDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEVAHMCKTDRVKYESTARSWTQKFAMG