Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063556.t1 | XP_003612270.1 | 79.4 | 180 | 10 | 2 | 1 | 154 | 7 | 185 | 2.60E-68 | 268.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063556.t1 | Q38853 | 60.8 | 125 | 47 | 2 | 31 | 154 | 59 | 182 | 9.9e-37 | 154.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063556.t1 | B7FGV4 | 79.4 | 180 | 10 | 2 | 1 | 154 | 7 | 185 | 1.8e-68 | 268.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052414 | MS.gene063556 | 0.800104 | 1.69E-48 | -1.69E-46 |
| MS.gene057396 | MS.gene063556 | 0.821965 | 3.05E-53 | -1.69E-46 |
| MS.gene058928 | MS.gene063556 | 0.806131 | 9.54E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTAGCTGGCCATAAGTATT+TGG | 0.181385 | 5.2:+14559949 | MS.gene063556:CDS |
| ACCTTACATCCAAATACTTA+TGG | 0.228445 | 5.2:-14559958 | None:intergenic |
| GTGTGGTTTAAAGGGGAATT+TGG | 0.241136 | 5.2:+14559871 | MS.gene063556:CDS |
| AAGCACGATGTGTGGTTTAA+AGG | 0.244947 | 5.2:+14559862 | MS.gene063556:intron |
| AGCACGATGTGTGGTTTAAA+GGG | 0.328888 | 5.2:+14559863 | MS.gene063556:intron |
| GATCCAACTTTGTACATATA+AGG | 0.335693 | 5.2:-14560314 | None:intergenic |
| TATGCTGCTTGGACTCAAAA+TGG | 0.348576 | 5.2:+14561201 | MS.gene063556:CDS |
| ATTAGAGTGTGACCTTGTTA+TGG | 0.398979 | 5.2:-14559543 | None:intergenic |
| GGGAATTTGGAGGAAGTTGT+TGG | 0.404121 | 5.2:+14559884 | MS.gene063556:CDS |
| GCCATAAGTATTTGGATGTA+AGG | 0.427420 | 5.2:+14559957 | MS.gene063556:CDS |
| TAGCTGGTGGTTATGCTGCT+TGG | 0.436274 | 5.2:+14561190 | MS.gene063556:CDS |
| ACACGCACTGGCACTGAAGT+TGG | 0.437130 | 5.2:-14559911 | None:intergenic |
| ACCAGCTATGTCTGTGAGTC+CGG | 0.440393 | 5.2:-14561175 | None:intergenic |
| GTGTTCTGCCGTAGAATTAG+AGG | 0.450733 | 5.2:-14559518 | None:intergenic |
| TATATGTACAAAGTTGGATC+AGG | 0.472445 | 5.2:+14560317 | MS.gene063556:CDS |
| TTTAATGCTGGACACGCTCC+TGG | 0.480736 | 5.2:+14560278 | MS.gene063556:CDS |
| ATACCTTATATGTACAAAGT+TGG | 0.502957 | 5.2:+14560311 | MS.gene063556:CDS |
| TGCATTGTATAGGGGTTCAC+CGG | 0.511879 | 5.2:+14561156 | MS.gene063556:intron |
| GCTCACGAGCTTCTTCTAGC+TGG | 0.514761 | 5.2:+14559935 | MS.gene063556:CDS |
| AGGGTTGTCAGCTTGGGAAA+AGG | 0.515357 | 5.2:+14560752 | MS.gene063556:intron |
| AGCTCGTGAGCTACACGCAC+TGG | 0.517304 | 5.2:-14559923 | None:intergenic |
| ATTCACGTCCTCTAATTCTA+CGG | 0.536092 | 5.2:+14559510 | MS.gene063556:CDS |
| ACCACGGAAGAGTTTAATGC+TGG | 0.548422 | 5.2:+14560266 | MS.gene063556:CDS |
| TGGTTTAAAGGGGAATTTGG+AGG | 0.552343 | 5.2:+14559874 | MS.gene063556:CDS |
| GCTTGGGAAAAGGTCTATGA+TGG | 0.555495 | 5.2:+14560762 | MS.gene063556:CDS |
| ACCGGACTCACAGACATAGC+TGG | 0.570324 | 5.2:+14561174 | MS.gene063556:CDS |
| TAAGGTATGTTAATCGCACC+AGG | 0.610995 | 5.2:-14560296 | None:intergenic |
| GCAAGCGGCGGAGACATCAA+AGG | 0.618010 | 5.2:-14559483 | None:intergenic |
| GCACGATGTGTGGTTTAAAG+GGG | 0.620601 | 5.2:+14559864 | MS.gene063556:intron |
| ATTGTGATTGTGCAGGACCA+CGG | 0.621639 | 5.2:+14560250 | MS.gene063556:intron |
| TCCAGCATTAAACTCTTCCG+TGG | 0.640037 | 5.2:-14560267 | None:intergenic |
| GGCAGAACACGACCATAACA+AGG | 0.641310 | 5.2:+14559531 | MS.gene063556:CDS |
| AGGACGTGAATGAAAGCAAG+CGG | 0.673997 | 5.2:-14559498 | None:intergenic |
| ACGTGAATGAAAGCAAGCGG+CGG | 0.698419 | 5.2:-14559495 | None:intergenic |
| GGACTCACAGACATAGCTGG+TGG | 0.717723 | 5.2:+14561177 | MS.gene063556:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAATCTTCCAATTTAATA+AGG | + | chr5.2:14559700-14559719 | MS.gene063556:intron | 15.0% |
| !! | ATATGAATAGATTAGAAAAA+AGG | - | chr5.2:14560529-14560548 | None:intergenic | 15.0% |
| !! | CAAAACATTAGTATATTAAT+CGG | - | chr5.2:14560181-14560200 | None:intergenic | 15.0% |
| !! | TAAATCTTCCAATTTAATAA+GGG | + | chr5.2:14559701-14559720 | MS.gene063556:intron | 15.0% |
| !! | AAATAACATACAAAATCTCA+TGG | - | chr5.2:14560687-14560706 | None:intergenic | 20.0% |
| !! | AGTATATAGATATAGAAGAA+TGG | - | chr5.2:14559573-14559592 | None:intergenic | 20.0% |
| !! | TAAAGTAACCAATTTAATCA+AGG | + | chr5.2:14559980-14559999 | MS.gene063556:intron | 20.0% |
| !! | TAAGAGATATATAATACTCT+TGG | + | chr5.2:14560418-14560437 | MS.gene063556:intron | 20.0% |
| !! | TATTTAAGCCTTGATTAAAT+TGG | - | chr5.2:14559991-14560010 | None:intergenic | 20.0% |
| !! | TTGTAATATTCTTAGTGTTT+AGG | + | chr5.2:14560367-14560386 | MS.gene063556:intron | 20.0% |
| !!! | AGTTGTTTTGATTAATCTAA+TGG | - | chr5.2:14560444-14560463 | None:intergenic | 20.0% |
| !!! | CATAATAGTTTTGTAGTTTT+AGG | - | chr5.2:14560054-14560073 | None:intergenic | 20.0% |
| !!! | CGATTAATATACTAATGTTT+TGG | + | chr5.2:14560179-14560198 | MS.gene063556:intron | 20.0% |
| !!! | TTAACTTCTTGAAAATTTTG+AGG | + | chr5.2:14561107-14561126 | MS.gene063556:intron | 20.0% |
| !!! | TTATTTTTGTTTACACTTTC+AGG | - | chr5.2:14561015-14561034 | None:intergenic | 20.0% |
| !!! | TTTTCATCAATAATTAGTCT+TGG | + | chr5.2:14561047-14561066 | MS.gene063556:intron | 20.0% |
| ! | AGATCAAAAAGAGATCAAAT+TGG | + | chr5.2:14560489-14560508 | MS.gene063556:intron | 25.0% |
| ! | ATACCTTATATGTACAAAGT+TGG | + | chr5.2:14560311-14560330 | MS.gene063556:CDS | 25.0% |
| ! | ATTAGAAAAAAGGACACTAA+TGG | - | chr5.2:14560519-14560538 | None:intergenic | 25.0% |
| ! | CATTAGCAGATGAATTAATT+TGG | - | chr5.2:14560719-14560738 | None:intergenic | 25.0% |
| ! | TAGATTAATCAAAACAACTG+AGG | + | chr5.2:14560445-14560464 | MS.gene063556:intron | 25.0% |
| ! | TATTGATGAAAAAAGTAGCA+CGG | - | chr5.2:14561039-14561058 | None:intergenic | 25.0% |
| ! | TTCTATGTCCCTTATTAAAT+TGG | - | chr5.2:14559712-14559731 | None:intergenic | 25.0% |
| !! | GTTAATAAAGTATCTATCAC+TGG | - | chr5.2:14561092-14561111 | None:intergenic | 25.0% |
| !! | TTAACAAAATTGCTGTTCTT+TGG | - | chr5.2:14560598-14560617 | None:intergenic | 25.0% |
| !!! | AATGTTTTGGAATTGTGATT+TGG | + | chr5.2:14560192-14560211 | MS.gene063556:intron | 25.0% |
| !!! | AATTGTTTTGCATTGTATAG+GGG | + | chr5.2:14561148-14561167 | MS.gene063556:intron | 25.0% |
| !!! | ACAATTGTTTTGCATTGTAT+AGG | + | chr5.2:14561146-14561165 | MS.gene063556:intron | 25.0% |
| !!! | CAATTGTTTTGCATTGTATA+GGG | + | chr5.2:14561147-14561166 | MS.gene063556:intron | 25.0% |
| AAACTCAAACAAGAAGTGAA+AGG | - | chr5.2:14560954-14560973 | None:intergenic | 30.0% | |
| AACTCAAACAAGAAGTGAAA+GGG | - | chr5.2:14560953-14560972 | None:intergenic | 30.0% | |
| AAGTATCTATCACTGGAAAT+TGG | - | chr5.2:14561085-14561104 | None:intergenic | 30.0% | |
| AATTATGTGTTCATGCATAC+AGG | + | chr5.2:14560567-14560586 | MS.gene063556:intron | 30.0% | |
| ACAATCACAATAAACAGGTA+TGG | - | chr5.2:14560241-14560260 | None:intergenic | 30.0% | |
| ACCTTACATCCAAATACTTA+TGG | - | chr5.2:14559961-14559980 | None:intergenic | 30.0% | |
| ATTATGTGTTCATGCATACA+GGG | + | chr5.2:14560568-14560587 | MS.gene063556:intron | 30.0% | |
| GATCCAACTTTGTACATATA+AGG | - | chr5.2:14560317-14560336 | None:intergenic | 30.0% | |
| TATATGTACAAAGTTGGATC+AGG | + | chr5.2:14560317-14560336 | MS.gene063556:CDS | 30.0% | |
| ! | ATCTTCTTTTCTAAACTGTG+AGG | - | chr5.2:14560629-14560648 | None:intergenic | 30.0% |
| ! | GAACAGCAATTTTGTTAAAG+AGG | + | chr5.2:14560600-14560619 | MS.gene063556:intron | 30.0% |
| !! | CTAATGTTATTGCTTTTGCA+GGG | + | chr5.2:14560733-14560752 | MS.gene063556:intron | 30.0% |
| !! | TCAACACTTTTTACACTTAC+CGG | - | chr5.2:14559767-14559786 | None:intergenic | 30.0% |
| ACTCAAACAAGAAGTGAAAG+GGG | - | chr5.2:14560952-14560971 | None:intergenic | 35.0% | |
| ATTAGAGTGTGACCTTGTTA+TGG | - | chr5.2:14559546-14559565 | None:intergenic | 35.0% | |
| ATTCACGTCCTCTAATTCTA+CGG | + | chr5.2:14559510-14559529 | MS.gene063556:CDS | 35.0% | |
| CAACTGAGGTTTGATAGAAT+AGG | + | chr5.2:14560459-14560478 | MS.gene063556:intron | 35.0% | |
| GCCATAAGTATTTGGATGTA+AGG | + | chr5.2:14559957-14559976 | MS.gene063556:CDS | 35.0% | |
| TAGCACTCAACTAAAATGCT+AGG | - | chr5.2:14560980-14560999 | None:intergenic | 35.0% | |
| ! | TTTTTACACTTACCGGAAGT+TGG | - | chr5.2:14559760-14559779 | None:intergenic | 35.0% |
| !! | GCTAATGTTATTGCTTTTGC+AGG | + | chr5.2:14560732-14560751 | MS.gene063556:intron | 35.0% |
| !!! | ATTTTGAGGTCAAAGCTTGT+TGG | + | chr5.2:14561121-14561140 | MS.gene063556:intron | 35.0% |
| !!! | TAGCATTTTAGTTGAGTGCT+AGG | + | chr5.2:14560979-14560998 | MS.gene063556:intron | 35.0% |
| !!! | TTTTTTCAAAGCACGATGTG+TGG | + | chr5.2:14559854-14559873 | MS.gene063556:intron | 35.0% |
| AAGCACGATGTGTGGTTTAA+AGG | + | chr5.2:14559862-14559881 | MS.gene063556:intron | 40.0% | |
| ACATACAAAATCTCATGGCG+CGG | - | chr5.2:14560682-14560701 | None:intergenic | 40.0% | |
| AGCACGATGTGTGGTTTAAA+GGG | + | chr5.2:14559863-14559882 | MS.gene063556:intron | 40.0% | |
| CCTGCACAATCACAATAAAC+AGG | - | chr5.2:14560246-14560265 | None:intergenic | 40.0% | |
| CCTGTTTATTGTGATTGTGC+AGG | + | chr5.2:14560243-14560262 | MS.gene063556:intron | 40.0% | |
| CTCAAACAAGAAGTGAAAGG+GGG | - | chr5.2:14560951-14560970 | None:intergenic | 40.0% | |
| GTGTGGTTTAAAGGGGAATT+TGG | + | chr5.2:14559871-14559890 | MS.gene063556:CDS | 40.0% | |
| TAAGGTATGTTAATCGCACC+AGG | - | chr5.2:14560299-14560318 | None:intergenic | 40.0% | |
| TCTAGCTGGCCATAAGTATT+TGG | + | chr5.2:14559949-14559968 | MS.gene063556:CDS | 40.0% | |
| TGGTTTAAAGGGGAATTTGG+AGG | + | chr5.2:14559874-14559893 | MS.gene063556:CDS | 40.0% | |
| ! | AACCAAAGTGTTCCAACTTC+CGG | + | chr5.2:14559745-14559764 | MS.gene063556:intron | 40.0% |
| ! | GTATGGAAAGTCGTGAGTTT+TGG | - | chr5.2:14560224-14560243 | None:intergenic | 40.0% |
| ! | TATGCTGCTTGGACTCAAAA+TGG | + | chr5.2:14561201-14561220 | MS.gene063556:CDS | 40.0% |
| ACCACGGAAGAGTTTAATGC+TGG | + | chr5.2:14560266-14560285 | MS.gene063556:CDS | 45.0% | |
| AGGACGTGAATGAAAGCAAG+CGG | - | chr5.2:14559501-14559520 | None:intergenic | 45.0% | |
| ATTGTGATTGTGCAGGACCA+CGG | + | chr5.2:14560250-14560269 | MS.gene063556:intron | 45.0% | |
| GCACGATGTGTGGTTTAAAG+GGG | + | chr5.2:14559864-14559883 | MS.gene063556:intron | 45.0% | |
| GCTTGGGAAAAGGTCTATGA+TGG | + | chr5.2:14560762-14560781 | MS.gene063556:CDS | 45.0% | |
| GGGAATTTGGAGGAAGTTGT+TGG | + | chr5.2:14559884-14559903 | MS.gene063556:CDS | 45.0% | |
| GTGTTCTGCCGTAGAATTAG+AGG | - | chr5.2:14559521-14559540 | None:intergenic | 45.0% | |
| TACCGGAAGTTGGAACACTT+TGG | - | chr5.2:14559750-14559769 | None:intergenic | 45.0% | |
| TCCAGCATTAAACTCTTCCG+TGG | - | chr5.2:14560270-14560289 | None:intergenic | 45.0% | |
| TGCATTGTATAGGGGTTCAC+CGG | + | chr5.2:14561156-14561175 | MS.gene063556:intron | 45.0% | |
| TGGACTGAAAGTCGAGTACA+CGG | + | chr5.2:14560090-14560109 | MS.gene063556:intron | 45.0% | |
| ! | TTTTGCAGGGTTGTCAGCTT+GGG | + | chr5.2:14560746-14560765 | MS.gene063556:intron | 45.0% |
| !!! | TATGTAATTTTTAAATTTAT+TGG | + | chr5.2:14560070-14560089 | MS.gene063556:intron | 5.0% |
| ACCAGCTATGTCTGTGAGTC+CGG | - | chr5.2:14561178-14561197 | None:intergenic | 50.0% | |
| ACGTGAATGAAAGCAAGCGG+CGG | - | chr5.2:14559498-14559517 | None:intergenic | 50.0% | |
| AGGGTTGTCAGCTTGGGAAA+AGG | + | chr5.2:14560752-14560771 | MS.gene063556:intron | 50.0% | |
| GGCAGAACACGACCATAACA+AGG | + | chr5.2:14559531-14559550 | MS.gene063556:CDS | 50.0% | |
| TAGCTGGTGGTTATGCTGCT+TGG | + | chr5.2:14561190-14561209 | MS.gene063556:CDS | 50.0% | |
| TTTAATGCTGGACACGCTCC+TGG | + | chr5.2:14560278-14560297 | MS.gene063556:CDS | 50.0% | |
| ! | CTTTTGCAGGGTTGTCAGCT+TGG | + | chr5.2:14560745-14560764 | MS.gene063556:intron | 50.0% |
| AAAATCTCATGGCGCGGCGA+AGG | - | chr5.2:14560676-14560695 | None:intergenic | 55.0% | |
| ACCGGACTCACAGACATAGC+TGG | + | chr5.2:14561174-14561193 | MS.gene063556:CDS | 55.0% | |
| GCTCACGAGCTTCTTCTAGC+TGG | + | chr5.2:14559935-14559954 | MS.gene063556:CDS | 55.0% | |
| GGACTCACAGACATAGCTGG+TGG | + | chr5.2:14561177-14561196 | MS.gene063556:CDS | 55.0% | |
| ! | ACACGCACTGGCACTGAAGT+TGG | - | chr5.2:14559914-14559933 | None:intergenic | 55.0% |
| AGCTCGTGAGCTACACGCAC+TGG | - | chr5.2:14559926-14559945 | None:intergenic | 60.0% | |
| GCAAGCGGCGGAGACATCAA+AGG | - | chr5.2:14559486-14559505 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 14559477 | 14561242 | 14559477 | ID=MS.gene063556 |
| chr5.2 | mRNA | 14559477 | 14561242 | 14559477 | ID=MS.gene063556.t1;Parent=MS.gene063556 |
| chr5.2 | exon | 14559477 | 14559552 | 14559477 | ID=MS.gene063556.t1.exon1;Parent=MS.gene063556.t1 |
| chr5.2 | CDS | 14559477 | 14559552 | 14559477 | ID=cds.MS.gene063556.t1;Parent=MS.gene063556.t1 |
| chr5.2 | exon | 14559865 | 14559978 | 14559865 | ID=MS.gene063556.t1.exon2;Parent=MS.gene063556.t1 |
| chr5.2 | CDS | 14559865 | 14559978 | 14559865 | ID=cds.MS.gene063556.t1;Parent=MS.gene063556.t1 |
| chr5.2 | exon | 14560265 | 14560357 | 14560265 | ID=MS.gene063556.t1.exon3;Parent=MS.gene063556.t1 |
| chr5.2 | CDS | 14560265 | 14560357 | 14560265 | ID=cds.MS.gene063556.t1;Parent=MS.gene063556.t1 |
| chr5.2 | exon | 14560606 | 14560660 | 14560606 | ID=MS.gene063556.t1.exon4;Parent=MS.gene063556.t1 |
| chr5.2 | CDS | 14560606 | 14560660 | 14560606 | ID=cds.MS.gene063556.t1;Parent=MS.gene063556.t1 |
| chr5.2 | exon | 14560754 | 14560807 | 14560754 | ID=MS.gene063556.t1.exon5;Parent=MS.gene063556.t1 |
| chr5.2 | CDS | 14560754 | 14560807 | 14560754 | ID=cds.MS.gene063556.t1;Parent=MS.gene063556.t1 |
| chr5.2 | exon | 14561168 | 14561242 | 14561168 | ID=MS.gene063556.t1.exon6;Parent=MS.gene063556.t1 |
| chr5.2 | CDS | 14561168 | 14561242 | 14561168 | ID=cds.MS.gene063556.t1;Parent=MS.gene063556.t1 |
| Gene Sequence |
| Protein sequence |
