Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063688.t1 | XP_003613292.1 | 94.6 | 240 | 13 | 0 | 1 | 240 | 64 | 303 | 1.50E-112 | 415.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063688.t1 | Q94JU2 | 42.9 | 240 | 132 | 2 | 2 | 240 | 69 | 304 | 5.7e-47 | 189.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063688.t1 | G7K2S5 | 94.6 | 240 | 13 | 0 | 1 | 240 | 64 | 303 | 1.1e-112 | 415.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052333 | MS.gene063688 | 0.807259 | 5.52E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGAAATCAGATTGGATAAT+TGG | 0.197562 | 5.2:+25356459 | MS.gene063688:CDS |
| GTGTTTATCACTAGCTCTTC+TGG | 0.211473 | 5.2:-25356944 | None:intergenic |
| TCCAATCTGATTTCCCTAAA+AGG | 0.218236 | 5.2:-25356452 | None:intergenic |
| CAGAAAATTGATGTTGAAAT+TGG | 0.226936 | 5.2:-25341339 | None:intergenic |
| TTCTGCAGATTACTTCTTCT+TGG | 0.233740 | 5.2:+25341357 | MS.gene063688:CDS |
| AGTGCAATGACCACTCTTAA+AGG | 0.236929 | 5.2:-25357570 | None:intergenic |
| ACTCATATGTTAATGACTTC+TGG | 0.288657 | 5.2:+25341485 | MS.gene063688:CDS |
| ATAGCAAGTATGAAGGTAAA+AGG | 0.300566 | 5.2:-25341560 | None:intergenic |
| GGCACAGTGGTCTCTATTGC+AGG | 0.308068 | 5.2:+25356345 | MS.gene063688:CDS |
| AGCAGCCAGGCAAAAGTTAT+TGG | 0.314638 | 5.2:+25356324 | MS.gene063688:CDS |
| TTGAACATAGCTACATATAT+TGG | 0.334507 | 5.2:-25357546 | None:intergenic |
| TACTTCTTCTTGGTTGCCTC+AGG | 0.379746 | 5.2:+25341367 | MS.gene063688:CDS |
| TCATACTTGCTATAATTTCA+AGG | 0.384041 | 5.2:+25341570 | MS.gene063688:CDS |
| TTGGGAGACAATCTTTATCT+TGG | 0.387204 | 5.2:+25357612 | MS.gene063688:CDS |
| ATATACAGACTTGGATTGTC+AGG | 0.424821 | 5.2:+25356915 | MS.gene063688:intron |
| ACAGATAGCAACTCTTTATC+AGG | 0.429389 | 5.2:-25357049 | None:intergenic |
| CAGAGGGAAATTCAAATGCT+TGG | 0.454003 | 5.2:+25357017 | MS.gene063688:CDS |
| TTAATGGCATCATTAATCAA+TGG | 0.480091 | 5.2:-25356399 | None:intergenic |
| TACCACAAAACAGCAACAAA+TGG | 0.500109 | 5.2:-25356966 | None:intergenic |
| AAAATTGATGTTGAAATTGG+AGG | 0.500522 | 5.2:-25341336 | None:intergenic |
| TGAAATTATAGCAAGTATGA+AGG | 0.526932 | 5.2:-25341567 | None:intergenic |
| AGTGGTCATTGCACTTAGCA+TGG | 0.527230 | 5.2:+25357578 | MS.gene063688:CDS |
| AATATAGAAATGGGAGCAAG+TGG | 0.531385 | 5.2:+25356423 | MS.gene063688:CDS |
| GAGACAATCTTTATCTTGGA+AGG | 0.532255 | 5.2:+25357616 | MS.gene063688:CDS |
| AACAATAGCTGAAGCCAACA+TGG | 0.534674 | 5.2:-25341525 | None:intergenic |
| ATTGATGTTGAAATTGGAGG+AGG | 0.546987 | 5.2:-25341333 | None:intergenic |
| GTGGTCATTGCACTTAGCAT+GGG | 0.547313 | 5.2:+25357579 | MS.gene063688:CDS |
| TAAATTCAGTTCTCCCATGT+TGG | 0.547322 | 5.2:+25341511 | MS.gene063688:CDS |
| CTCTGCAATGAGAGCTACAA+TGG | 0.554415 | 5.2:-25356999 | None:intergenic |
| ACATGGGCATGTCGTAAGAA+AGG | 0.565963 | 5.2:+25357522 | MS.gene063688:CDS |
| GCACAGTGGTCTCTATTGCA+GGG | 0.571242 | 5.2:+25356346 | MS.gene063688:CDS |
| CACAGTGGTCTCTATTGCAG+GGG | 0.584216 | 5.2:+25356347 | MS.gene063688:CDS |
| TATGTTCAAACCTTTAAGAG+TGG | 0.597273 | 5.2:+25357560 | MS.gene063688:CDS |
| TGAAGAATGTTATACTCACA+TGG | 0.605157 | 5.2:+25357505 | MS.gene063688:CDS |
| GCAAGTATGAAGGTAAAAGG+AGG | 0.605835 | 5.2:-25341557 | None:intergenic |
| ATTGTAGCTCTCATTGCAGA+GGG | 0.608307 | 5.2:+25357001 | MS.gene063688:CDS |
| TGGTCATTGCACTTAGCATG+GGG | 0.620952 | 5.2:+25357580 | MS.gene063688:CDS |
| CAAGTATGAAGGTAAAAGGA+GGG | 0.622141 | 5.2:-25341556 | None:intergenic |
| TATACAGACTTGGATTGTCA+GGG | 0.626722 | 5.2:+25356916 | MS.gene063688:intron |
| CATTGTAGCTCTCATTGCAG+AGG | 0.632723 | 5.2:+25357000 | MS.gene063688:CDS |
| GAAGAATGTTATACTCACAT+GGG | 0.641061 | 5.2:+25357506 | MS.gene063688:CDS |
| ACAATAGCTGAAGCCAACAT+GGG | 0.645656 | 5.2:-25341524 | None:intergenic |
| GGCAAAAGTTATTGGCACAG+TGG | 0.705210 | 5.2:+25356332 | MS.gene063688:CDS |
| GGTCATTGCACTTAGCATGG+GGG | 0.733530 | 5.2:+25357581 | MS.gene063688:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 25341331 | 25357637 | 25341331 | ID=MS.gene063688 |
| chr5.2 | mRNA | 25341331 | 25357637 | 25341331 | ID=MS.gene063688.t1;Parent=MS.gene063688 |
| chr5.2 | exon | 25341331 | 25341388 | 25341331 | ID=MS.gene063688.t1.exon1;Parent=MS.gene063688.t1 |
| chr5.2 | CDS | 25341331 | 25341388 | 25341331 | ID=cds.MS.gene063688.t1;Parent=MS.gene063688.t1 |
| chr5.2 | exon | 25341478 | 25341591 | 25341478 | ID=MS.gene063688.t1.exon2;Parent=MS.gene063688.t1 |
| chr5.2 | CDS | 25341478 | 25341591 | 25341478 | ID=cds.MS.gene063688.t1;Parent=MS.gene063688.t1 |
| chr5.2 | exon | 25356296 | 25356536 | 25356296 | ID=MS.gene063688.t1.exon3;Parent=MS.gene063688.t1 |
| chr5.2 | CDS | 25356296 | 25356536 | 25356296 | ID=cds.MS.gene063688.t1;Parent=MS.gene063688.t1 |
| chr5.2 | exon | 25356923 | 25357081 | 25356923 | ID=MS.gene063688.t1.exon4;Parent=MS.gene063688.t1 |
| chr5.2 | CDS | 25356923 | 25357081 | 25356923 | ID=cds.MS.gene063688.t1;Parent=MS.gene063688.t1 |
| chr5.2 | exon | 25357486 | 25357637 | 25357486 | ID=MS.gene063688.t1.exon5;Parent=MS.gene063688.t1 |
| chr5.2 | CDS | 25357486 | 25357637 | 25357486 | ID=cds.MS.gene063688.t1;Parent=MS.gene063688.t1 |
| Gene Sequence |
| Protein sequence |
