AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene063911

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063911.t1 MTR_8g042900 97.246 581 16 0 1 581 1 581 0.0 1176
MS.gene063911.t1 MTR_3g010780 72.165 582 153 4 1 581 1 574 0.0 845
MS.gene063911.t1 MTR_8g023150 48.744 597 272 13 1 581 4 582 3.14e-166 486
MS.gene063911.t1 MTR_4g087980 46.170 483 231 7 109 581 53 516 2.83e-144 427
MS.gene063911.t1 MTR_7g112590 40.941 574 304 10 23 570 8 572 2.04e-143 427
MS.gene063911.t1 MTR_1g008140 41.497 588 302 15 23 581 33 607 2.90e-143 428
MS.gene063911.t1 MTR_1g083510 40.992 605 291 16 17 579 1 581 1.42e-141 422
MS.gene063911.t1 MTR_3g091380 40.893 582 311 12 22 581 27 597 2.07e-140 420
MS.gene063911.t1 MTR_7g050950 43.883 515 272 9 74 581 49 553 5.40e-138 412
MS.gene063911.t1 MTR_2g044880 45.011 451 227 5 141 581 86 525 4.51e-136 406
MS.gene063911.t1 MTR_7g108905 43.160 519 265 9 77 577 46 552 7.25e-136 407
MS.gene063911.t1 MTR_8g104655 49.036 467 205 9 135 581 88 541 2.92e-135 405
MS.gene063911.t1 MTR_7g112580 40.987 527 288 7 65 581 29 542 1.41e-134 403
MS.gene063911.t1 MTR_8g023310 43.019 530 270 9 76 581 68 589 1.63e-133 402
MS.gene063911.t1 MTR_4g087970 44.643 448 222 7 147 581 83 517 3.14e-133 399
MS.gene063911.t1 MTR_1g083470 37.986 566 328 9 26 577 10 566 3.51e-133 401
MS.gene063911.t1 MTR_1g083500 37.986 566 328 9 26 577 10 566 3.51e-133 401
MS.gene063911.t1 MTR_4g124550 38.294 598 330 15 3 574 2 586 9.64e-133 400
MS.gene063911.t1 MTR_8g104645 55.587 349 143 5 241 581 28 372 3.68e-132 391
MS.gene063911.t1 MTR_7g050870 43.327 517 273 11 74 581 49 554 7.42e-132 397
MS.gene063911.t1 MTR_4g025690 45.661 484 223 12 107 581 61 513 1.18e-131 395
MS.gene063911.t1 MTR_1g054880 44.821 502 242 12 110 581 62 558 2.01e-131 395
MS.gene063911.t1 MTR_1g083490 38.179 571 325 11 26 577 10 571 2.71e-131 396
MS.gene063911.t1 MTR_4g025730 45.809 513 243 17 74 581 27 509 1.63e-130 392
MS.gene063911.t1 MTR_4g005170 38.070 570 325 11 27 577 11 571 2.54e-130 395
MS.gene063911.t1 MTR_1g088020 42.272 537 268 13 77 581 29 555 6.09e-128 387
MS.gene063911.t1 MTR_8g042910 42.086 537 274 9 80 581 78 612 6.45e-127 386
MS.gene063911.t1 MTR_4g025670 43.621 486 233 11 113 581 28 489 3.07e-126 380
MS.gene063911.t1 MTR_1g083520 42.125 527 268 10 71 581 23 528 6.65e-126 380
MS.gene063911.t1 MTR_3g010770 40.734 545 276 7 78 581 37 575 3.42e-125 380
MS.gene063911.t1 MTR_4g005180 37.255 561 324 11 36 577 2 553 1.86e-124 378
MS.gene063911.t1 MTR_1g088225 45.570 474 222 12 140 581 84 553 1.95e-124 377
MS.gene063911.t1 MTR_7g108875 41.219 558 273 14 61 581 25 564 2.52e-124 377
MS.gene063911.t1 MTR_7g050980 44.295 447 232 8 143 581 118 555 1.07e-123 375
MS.gene063911.t1 MTR_2g044810 40.000 510 282 8 74 577 39 530 2.06e-123 374
MS.gene063911.t1 MTR_1g086190 38.148 540 303 11 60 577 29 559 2.07e-123 375
MS.gene063911.t1 MTR_4g124520 41.700 506 253 9 74 570 38 510 1.48e-122 372
MS.gene063911.t1 MTR_1g086210 38.686 548 292 13 65 577 27 565 9.11e-122 371
MS.gene063911.t1 MTR_7g108900 40.074 539 276 10 77 581 35 560 9.29e-121 368
MS.gene063911.t1 MTR_7g108890 41.035 541 268 14 77 581 37 562 2.29e-119 365
MS.gene063911.t1 MTR_7g053360 40.918 523 271 12 73 581 29 527 1.16e-118 362
MS.gene063911.t1 MTR_4g120280 37.391 575 320 11 24 577 13 568 5.39e-118 362
MS.gene063911.t1 MTR_3g008700 44.898 441 219 9 150 581 102 527 5.42e-117 357
MS.gene063911.t1 MTR_3g008640 44.898 441 219 9 150 581 102 527 1.13e-116 357
MS.gene063911.t1 MTR_3g008720 44.898 441 219 9 150 581 102 527 1.34e-116 356
MS.gene063911.t1 MTR_2g436040 36.106 565 312 12 26 577 14 542 4.25e-116 358
MS.gene063911.t1 MTR_3g033690 40.918 523 270 13 73 581 29 526 4.71e-116 355
MS.gene063911.t1 MTR_4g120290 36.458 576 324 10 24 577 13 568 1.67e-114 353
MS.gene063911.t1 MTR_4g035980 42.451 457 233 10 140 581 98 539 3.77e-110 340
MS.gene063911.t1 MTR_7g110440 37.729 546 305 12 63 581 29 566 5.79e-109 338
MS.gene063911.t1 MTR_2g436050 36.189 572 320 12 26 577 14 560 4.38e-108 335
MS.gene063911.t1 MTR_0458s0030 58.077 260 105 1 326 581 55 314 1.74e-106 323
MS.gene063911.t1 MTR_1g088040 58.077 260 105 1 326 581 55 314 1.74e-106 323
MS.gene063911.t1 MTR_7g110490 35.911 582 327 13 27 581 4 566 4.17e-106 331
MS.gene063911.t1 MTR_7g110510 35.911 582 327 13 27 581 4 566 4.17e-106 331
MS.gene063911.t1 MTR_8g104580 40.381 525 238 14 80 581 28 500 7.54e-105 325
MS.gene063911.t1 MTR_7g110560 34.974 569 326 12 26 577 4 545 2.79e-104 325
MS.gene063911.t1 MTR_2g044660 48.197 305 156 2 271 574 322 625 1.60e-97 310
MS.gene063911.t1 MTR_0618s0020 58.590 227 89 2 360 581 2 228 3.35e-93 285
MS.gene063911.t1 MTR_7g110420 38.655 476 256 12 142 581 103 578 1.39e-91 293
MS.gene063911.t1 MTR_5g083620 42.286 350 190 8 240 581 11 356 1.02e-89 281
MS.gene063911.t1 MTR_8g093460 35.000 520 301 14 74 573 41 543 3.34e-88 284
MS.gene063911.t1 MTR_2g044810 50.000 246 119 1 336 577 216 461 8.63e-88 280
MS.gene063911.t1 MTR_7g110430 36.422 464 239 9 141 581 102 532 7.59e-86 277
MS.gene063911.t1 MTR_8g088610 41.141 333 182 7 253 577 46 372 2.03e-84 268
MS.gene063911.t1 MTR_3g095920 32.348 575 325 19 27 570 12 553 2.50e-81 266
MS.gene063911.t1 MTR_7g107180 40.705 312 168 7 276 581 7 307 1.48e-69 227
MS.gene063911.t1 MTR_0458s0010 35.319 470 241 15 140 581 84 518 1.21e-68 231
MS.gene063911.t1 MTR_7g111560 31.014 503 301 13 105 579 70 554 1.01e-63 219
MS.gene063911.t1 MTR_2g105560 35.799 338 193 8 255 581 15 339 2.94e-63 211
MS.gene063911.t1 MTR_2g039530 38.941 321 153 9 243 546 22 316 3.98e-55 190
MS.gene063911.t1 MTR_6g088810 36.913 298 167 6 284 570 90 377 2.68e-51 181
MS.gene063911.t1 MTR_7g102600 31.722 331 203 7 254 570 72 393 2.52e-50 179
MS.gene063911.t1 MTR_6g012800 33.775 302 181 5 276 567 6 298 7.64e-50 175
MS.gene063911.t1 MTR_5g083590 33.010 309 153 5 274 577 5 264 1.09e-49 173
MS.gene063911.t1 MTR_6g024165 34.251 327 179 11 260 570 28 334 1.67e-49 175
MS.gene063911.t1 MTR_6g042660 35.036 274 159 7 276 544 37 296 7.62e-49 172
MS.gene063911.t1 MTR_8g102380 35.667 300 170 5 284 570 88 377 1.97e-48 173
MS.gene063911.t1 MTR_7g092780 33.443 305 186 6 276 570 90 387 2.08e-48 173
MS.gene063911.t1 MTR_1g033050 32.704 318 196 5 260 570 2 308 2.94e-47 168
MS.gene063911.t1 MTR_1g086540 32.075 318 192 7 267 570 37 344 1.45e-45 164
MS.gene063911.t1 MTR_7g011530 31.544 298 185 5 278 565 8 296 2.28e-43 157
MS.gene063911.t1 MTR_5g095490 33.444 302 177 7 282 570 67 357 2.78e-42 155
MS.gene063911.t1 MTR_6g042690 34.767 279 158 8 276 544 39 303 7.42e-42 154
MS.gene063911.t1 MTR_5g012600 30.769 312 194 6 276 575 63 364 8.40e-39 146
MS.gene063911.t1 MTR_8g102380 37.500 248 133 5 284 518 88 326 1.01e-38 145
MS.gene063911.t1 MTR_5g093970 31.438 299 189 6 276 570 43 329 1.89e-38 144
MS.gene063911.t1 MTR_4g124740 29.268 328 195 12 258 570 34 339 3.75e-37 141
MS.gene063911.t1 MTR_2g033970 30.312 320 196 8 263 572 46 348 6.08e-37 140
MS.gene063911.t1 MTR_5g088280 29.492 295 193 5 276 570 39 318 3.84e-36 138
MS.gene063911.t1 MTR_5g088290 30.405 296 190 5 276 570 48 328 3.91e-36 138
MS.gene063911.t1 MTR_5g088300 29.831 295 192 4 276 570 37 316 2.53e-34 133
MS.gene063911.t1 MTR_2g436070 30.514 331 198 10 26 347 14 321 2.77e-34 134
MS.gene063911.t1 MTR_2g073390 29.276 304 193 8 276 570 71 361 5.81e-32 127
MS.gene063911.t1 MTR_8g036225 28.289 304 195 6 276 570 33 322 6.82e-32 126
MS.gene063911.t1 MTR_4g074290 28.808 302 197 7 276 570 71 361 9.72e-32 126
MS.gene063911.t1 MTR_4g051810 28.808 302 187 6 276 570 37 317 1.54e-30 122
MS.gene063911.t1 MTR_2g436600 27.726 321 194 11 263 572 45 338 1.30e-28 117
MS.gene063911.t1 MTR_0009s0350 27.483 302 201 7 276 570 71 361 3.81e-28 116
MS.gene063911.t1 MTR_0009s0340 27.483 302 201 7 276 570 71 361 3.81e-28 116
MS.gene063911.t1 MTR_6g025590 34.831 178 105 6 260 429 36 210 5.55e-22 95.5
MS.gene063911.t1 MTR_0408s0030 40.860 93 50 2 452 544 36 123 3.33e-17 79.3
MS.gene063911.t1 MTR_7g102580 38.462 130 55 5 319 429 29 152 1.97e-16 77.0
MS.gene063911.t1 MTR_4g130790 27.755 245 152 7 279 513 25 254 6.18e-16 78.6
MS.gene063911.t1 MTR_8g104765 28.638 213 131 5 29 228 3 207 1.00e-15 77.0
MS.gene063911.t1 MTR_0036s0140 28.235 255 136 9 4 244 7 228 2.50e-15 75.9
MS.gene063911.t1 MTR_4g120830 28.169 213 123 5 29 228 3 198 6.20e-15 74.3
MS.gene063911.t1 MTR_4g120825 27.700 213 124 5 29 228 3 198 5.64e-14 71.6
MS.gene063911.t1 MTR_7g108790 31.111 180 103 5 61 237 29 190 9.15e-14 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063911.t1 AT3G10710 57.366 577 224 9 7 581 5 561 0.0 670
MS.gene063911.t1 AT5G04960 56.507 584 224 9 6 581 3 564 0.0 645
MS.gene063911.t1 AT1G53840 46.399 597 277 14 1 574 4 580 3.62e-162 475
MS.gene063911.t1 AT3G05610 38.644 590 322 11 6 581 2 565 2.50e-144 432
MS.gene063911.t1 AT5G27870 39.492 590 306 11 6 577 2 558 3.90e-143 431
MS.gene063911.t1 AT3G14300 47.784 519 236 13 73 571 456 959 5.14e-143 438
MS.gene063911.t1 AT3G14300 34.118 255 147 7 5 247 9 254 1.13e-27 119
MS.gene063911.t1 AT3G14300 36.257 171 87 6 73 228 268 431 8.19e-15 78.6
MS.gene063911.t1 AT5G53370 41.567 587 307 13 12 581 18 585 7.69e-142 423
MS.gene063911.t1 AT5G53370 41.567 587 307 13 12 581 54 621 2.12e-141 423
MS.gene063911.t1 AT5G49180 41.519 566 298 11 25 571 9 560 2.51e-138 414
MS.gene063911.t1 AT3G05620 40.490 531 279 7 69 581 30 541 3.96e-137 410
MS.gene063911.t1 AT3G43270 45.916 453 230 6 142 581 75 525 1.03e-136 408
MS.gene063911.t1 AT1G11580 41.682 547 296 9 43 581 26 557 3.26e-135 405
MS.gene063911.t1 AT3G49220 40.909 572 312 12 22 581 39 596 5.93e-135 406
MS.gene063911.t1 AT1G11580 41.682 547 296 9 43 581 47 578 9.49e-135 405
MS.gene063911.t1 AT2G26450 38.080 625 326 14 3 581 2 611 1.34e-134 405
MS.gene063911.t1 AT3G49220 40.909 572 312 12 22 581 84 641 6.23e-134 405
MS.gene063911.t1 AT4G33220 41.948 503 256 8 105 581 31 523 1.33e-132 397
MS.gene063911.t1 AT1G53830 42.058 554 277 13 62 581 44 587 1.91e-131 397
MS.gene063911.t1 AT3G14310 41.145 559 273 13 67 581 46 592 4.61e-131 396
MS.gene063911.t1 AT4G33230 39.418 619 321 16 3 581 2 606 6.11e-131 396
MS.gene063911.t1 AT4G02330 42.287 551 269 14 73 581 28 571 6.71e-131 395
MS.gene063911.t1 AT1G23200 44.402 527 244 14 80 574 38 547 4.58e-128 387
MS.gene063911.t1 AT2G47550 44.946 465 227 9 144 581 96 558 1.14e-124 378
MS.gene063911.t1 AT4G33220 51.786 336 150 4 253 581 72 402 1.89e-124 372
MS.gene063911.t1 AT1G02810 39.787 563 280 12 70 581 23 577 2.78e-124 378
MS.gene063911.t1 AT3G06830 38.361 537 305 9 64 581 39 568 6.62e-124 377
MS.gene063911.t1 AT2G26440 40.971 515 273 9 74 570 35 536 1.40e-122 372
MS.gene063911.t1 AT2G45220 44.639 513 246 16 76 581 30 511 1.29e-121 369
MS.gene063911.t1 AT5G51490 41.506 518 268 14 80 581 38 536 2.35e-120 366
MS.gene063911.t1 AT5G51500 40.962 520 282 13 73 581 35 540 3.43e-117 358
MS.gene063911.t1 AT2G43050 42.198 455 235 7 136 581 81 516 6.37e-117 357
MS.gene063911.t1 AT4G03930 43.596 445 232 7 142 581 94 524 6.14e-115 352
MS.gene063911.t1 AT3G60730 41.704 446 227 8 157 581 86 519 8.92e-114 349
MS.gene063911.t1 AT3G59010 42.495 473 243 12 124 581 70 528 2.38e-112 345
MS.gene063911.t1 AT1G11590 42.630 441 236 7 145 581 97 524 2.86e-112 345
MS.gene063911.t1 AT3G10720 40.471 467 246 7 147 581 149 615 1.62e-111 346
MS.gene063911.t1 AT3G27980 44.581 406 205 7 182 581 106 497 2.00e-111 342
MS.gene063911.t1 AT5G04970 40.043 467 248 7 147 581 154 620 1.29e-109 342
MS.gene063911.t1 AT4G02320 38.641 471 252 7 133 581 63 518 5.00e-109 337
MS.gene063911.t1 AT3G47400 37.618 529 305 8 68 581 76 594 2.60e-108 337
MS.gene063911.t1 AT4G02300 39.602 452 252 8 139 577 85 528 1.21e-104 326
MS.gene063911.t1 AT4G00190 40.858 443 228 8 144 581 61 474 3.92e-102 317
MS.gene063911.t1 AT5G20860 38.063 444 247 9 150 581 111 538 1.67e-101 318
MS.gene063911.t1 AT3G62170 33.846 585 329 12 30 570 7 577 2.31e-97 308
MS.gene063911.t1 AT3G10720 52.510 259 119 1 327 581 1 259 1.29e-95 293
MS.gene063911.t1 AT4G15980 45.509 334 176 3 246 573 361 694 2.03e-93 301
MS.gene063911.t1 AT4G15980 32.121 165 98 6 74 233 46 201 3.63e-15 79.3
MS.gene063911.t1 AT2G47040 33.002 603 320 13 29 570 5 584 2.46e-93 298
MS.gene063911.t1 AT4G15980 45.509 334 176 3 246 573 371 704 4.95e-93 301
MS.gene063911.t1 AT4G15980 32.121 165 98 6 74 233 56 211 3.94e-15 79.3
MS.gene063911.t1 AT2G47030 32.886 596 323 13 29 570 5 577 4.35e-92 295
MS.gene063911.t1 AT5G09760 36.398 533 287 15 67 569 36 546 3.92e-89 286
MS.gene063911.t1 AT5G09760 36.398 533 287 15 67 569 62 572 9.68e-89 286
MS.gene063911.t1 AT1G11370 48.810 252 125 1 267 514 37 288 3.77e-84 264
MS.gene063911.t1 AT5G64640 32.590 583 339 15 24 569 32 597 2.22e-83 272
MS.gene063911.t1 AT5G20860 35.349 430 222 8 150 567 111 496 1.30e-82 268
MS.gene063911.t1 AT1G05310 33.115 305 187 5 276 570 91 388 4.41e-52 183
MS.gene063911.t1 AT2G36710 34.528 307 185 5 273 570 86 385 1.85e-49 177
MS.gene063911.t1 AT5G19730 35.082 305 177 6 277 570 87 381 7.24e-49 174
MS.gene063911.t1 AT1G44980 44.444 189 104 1 253 440 54 242 2.84e-45 160
MS.gene063911.t1 AT2G21610 30.945 307 195 6 276 580 51 342 9.92e-45 162
MS.gene063911.t1 AT2G21610 30.945 307 195 6 276 580 56 347 1.44e-44 162
MS.gene063911.t1 AT3G29090 31.683 303 188 4 276 568 7 300 3.72e-44 160
MS.gene063911.t1 AT2G36700 31.667 300 188 5 278 570 44 333 3.43e-42 155
MS.gene063911.t1 AT5G55590 34.202 307 180 7 276 570 82 378 5.94e-42 155
MS.gene063911.t1 AT5G47500 28.134 359 232 7 224 570 13 357 1.56e-40 151
MS.gene063911.t1 AT5G18990 30.968 310 192 6 272 570 25 323 1.86e-40 150
MS.gene063911.t1 AT3G24130 30.968 310 190 7 273 570 31 328 2.20e-40 150
MS.gene063911.t1 AT3G17060 28.618 304 197 6 276 571 46 337 6.35e-40 149
MS.gene063911.t1 AT2G19150 31.579 304 187 6 276 570 41 332 1.22e-39 148
MS.gene063911.t1 AT3G49220 31.884 345 214 10 22 359 84 414 9.53e-39 147
MS.gene063911.t1 AT5G55590 35.714 252 142 6 276 515 82 325 6.96e-37 140
MS.gene063911.t1 AT5G07420 26.923 364 236 8 224 570 5 355 2.57e-35 136
MS.gene063911.t1 AT5G26810 28.617 311 195 6 276 570 34 333 3.24e-35 135
MS.gene063911.t1 AT5G07430 26.027 365 238 8 224 570 5 355 1.53e-34 134
MS.gene063911.t1 AT2G19150 32.530 249 149 5 276 515 41 279 3.56e-33 129
MS.gene063911.t1 AT2G47280 28.571 308 192 7 276 570 35 327 6.37e-32 126
MS.gene063911.t1 AT5G61680 27.632 304 197 7 276 570 69 358 1.51e-30 123
MS.gene063911.t1 AT1G69940 25.926 297 197 7 283 570 73 355 3.72e-28 116
MS.gene063911.t1 AT5G07410 25.926 297 197 7 283 570 73 355 1.20e-27 115
MS.gene063911.t1 AT4G25260 29.949 197 117 6 41 228 10 194 2.58e-12 66.6
MS.gene063911.t1 AT1G62760 31.098 164 92 5 76 228 148 301 4.14e-12 67.8

Find 105 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAACCATGTCTTCCTTTA+AGG 0.044203 8.1:-61456224 None:intergenic
TAAGGATTTGAACTTGAATT+TGG 0.174635 8.1:-61455819 MS.gene063911:CDS
GACTTTACCATAGGCCTTAA+AGG 0.212569 8.1:+61456210 None:intergenic
TTGAATAGCTCTTTGTCTTA+TGG 0.218218 8.1:-61455734 MS.gene063911:CDS
ATGAATGTTACTATTGTTTC+TGG 0.218323 8.1:-61455113 MS.gene063911:CDS
GATCAATTGTAAGGATCTTT+TGG 0.225160 8.1:-61455774 MS.gene063911:CDS
AGTTCTGTTAAAACATATTT+AGG 0.227942 8.1:-61454323 MS.gene063911:CDS
ATCTCTTTCAAGTTCAATTA+AGG 0.244852 8.1:-61456008 MS.gene063911:CDS
AATTGCAATGTGTCGCCTTT+TGG 0.251914 8.1:-61454353 MS.gene063911:CDS
AAATGGAATGTTATGATTAT+TGG 0.258364 8.1:-61455143 MS.gene063911:CDS
GGATTTATTATGAGAATGTT+AGG 0.275021 8.1:-61455178 MS.gene063911:CDS
GAAATTCCTGTGTTCATGTT+AGG 0.284061 8.1:+61454383 None:intergenic
GAAACTTGGTTGAGTGCTTC+AGG 0.293398 8.1:-61455674 MS.gene063911:intron
CCACTTGACCCTATTCTTCA+TGG 0.298592 8.1:+61454151 None:intergenic
TTAGGGTGGAAAAGACTAAA+TGG 0.331655 8.1:-61455160 MS.gene063911:CDS
CCTTAGTCCTCTTGTTCAAC+CGG 0.337493 8.1:-61455942 MS.gene063911:CDS
GTTTAATATTCAGCTGTGTT+TGG 0.340708 8.1:-61454704 MS.gene063911:intron
TGTTGCATTCGCGGTAGAAT+TGG 0.341900 8.1:+61454528 None:intergenic
TACCAGCAAACATGCATTGA+TGG 0.346136 8.1:-61455539 MS.gene063911:CDS
TTCGAACTTCGAACATGGCT+TGG 0.346240 8.1:+61456176 None:intergenic
GATAGATGGATCCCTGCTTC+TGG 0.358027 8.1:-61454062 MS.gene063911:CDS
AGCTCATAAGTTTACAGTTA+AGG 0.369384 8.1:-61454099 MS.gene063911:CDS
GTTACCTTGGGTTGGAGATT+CGG 0.370568 8.1:-61454225 MS.gene063911:CDS
TTTAATATTCAGCTGTGTTT+GGG 0.376483 8.1:-61454703 MS.gene063911:intron
GTTATTGTGTTCTGCTGTCC+TGG 0.383824 8.1:+61454425 None:intergenic
AACCATCAATGCATGTTTGC+TGG 0.390697 8.1:+61455537 None:intergenic
TCTTTATGATGAAGCCAGTT+TGG 0.390868 8.1:+61455353 None:intergenic
GGAAGCTTTGATCAATTGTA+AGG 0.395095 8.1:-61455783 MS.gene063911:CDS
TCGAACTTCGAACATGGCTT+GGG 0.401179 8.1:+61456177 None:intergenic
GGTGTTTATGCAATCGAACT+TGG 0.413873 8.1:-61454273 MS.gene063911:CDS
ATGTCTTCCTTTAAGGCCTA+TGG 0.419251 8.1:-61456217 MS.gene063911:CDS
GCTTAACAAGGCTGCATCTT+CGG 0.420371 8.1:-61455420 MS.gene063911:CDS
GTCGGCTTTCTTCTTCAAAT+CGG 0.425906 8.1:+61455307 None:intergenic
TTCCGTAGATGTTGCATTCG+CGG 0.433043 8.1:+61454519 None:intergenic
ATCAAAATGAAGAACCAAAC+TGG 0.433147 8.1:-61455367 MS.gene063911:CDS
TATGCTGAATTTCAGAATGT+TGG 0.433530 8.1:-61454185 MS.gene063911:CDS
TTAAATGTTGCACCAGAAGC+AGG 0.437960 8.1:+61454050 None:intergenic
GTGGTGCTTCCATGAAGAAT+AGG 0.438477 8.1:-61454160 MS.gene063911:CDS
ACCCAAGGTAACCATCCATT+TGG 0.439713 8.1:+61454236 None:intergenic
ACCGTGATCTATGTGAAGAA+AGG 0.447852 8.1:-61455200 MS.gene063911:CDS
TTGTCCACAATTGTCCTCAT+TGG 0.452200 8.1:-61456121 MS.gene063911:CDS
GTGTTCTGCTGTCCTGGCAT+AGG 0.453171 8.1:+61454431 None:intergenic
TCCAAATGGATGGTTACCTT+GGG 0.459205 8.1:-61454237 MS.gene063911:CDS
GTCCAAATGGATGGTTACCT+TGG 0.461469 8.1:-61454238 MS.gene063911:CDS
AAGCACTCAACCAAGTTTCA+AGG 0.464276 8.1:+61455678 None:intergenic
AATTTCATTGCAAGAGACAT+AGG 0.466408 8.1:-61454677 MS.gene063911:CDS
TCAGCATAAAATATAGTATC+CGG 0.473076 8.1:+61454200 None:intergenic
TCCCTAATAAAAGTGACAAT+AGG 0.479786 8.1:-61455223 MS.gene063911:CDS
TGGTGCTTCCATGAAGAATA+GGG 0.495686 8.1:-61454159 MS.gene063911:CDS
TTGTCGCGAAAGACGGGAGT+GGG 0.497689 8.1:-61455280 MS.gene063911:CDS
AAACATATTTAGGTAGACCT+TGG 0.501761 8.1:-61454313 MS.gene063911:CDS
CCGTGATCTATGTGAAGAAA+GGG 0.502068 8.1:-61455199 MS.gene063911:CDS
GATTTATTATGAGAATGTTA+GGG 0.506310 8.1:-61455177 MS.gene063911:CDS
CAGAACACAATAACAGCACA+AGG 0.511799 8.1:-61454416 MS.gene063911:CDS
TGTCCTGGCATAGGTTGTCT+TGG 0.512818 8.1:+61454440 None:intergenic
ACATTGTTGTCGCGAAAGAC+GGG 0.518230 8.1:-61455286 MS.gene063911:CDS
AATGGATGGTTACCTTGGGT+TGG 0.521909 8.1:-61454233 MS.gene063911:CDS
GACATTGTTGTCGCGAAAGA+CGG 0.522332 8.1:-61455287 MS.gene063911:CDS
ACAGAACTCAGATTTCCAAA+AGG 0.528200 8.1:+61454338 None:intergenic
AATAGCTCTTTGTCTTATGG+AGG 0.533210 8.1:-61455731 MS.gene063911:CDS
TGCTGACCCGGTTGAACAAG+AGG 0.534157 8.1:+61455935 None:intergenic
GTGTTTATGCAATCGAACTT+GGG 0.536740 8.1:-61454272 MS.gene063911:CDS
TTTCCTTCGAACTTCGAACA+TGG 0.537460 8.1:+61456171 None:intergenic
GAAGAATAGGGTCAAGTGGA+AGG 0.538939 8.1:-61454147 MS.gene063911:CDS
GACAATTTGAACAACTCCTC+GGG 0.540472 8.1:+61455905 None:intergenic
TCAAATCCTTAAAAGTTCCA+TGG 0.541268 8.1:+61455831 None:intergenic
GTTCAAATTGTCGATTCAAG+TGG 0.542567 8.1:-61455894 MS.gene063911:CDS
TTGTCATTAGTGCTACTGCT+TGG 0.546362 8.1:+61454624 None:intergenic
AATGTTATGATTATTGGTGA+TGG 0.547054 8.1:-61455137 MS.gene063911:CDS
GTTGTTGAGTAATTCTTCCA+AGG 0.554426 8.1:+61454296 None:intergenic
CTTGTTAAGCCATGTGATGA+TGG 0.554658 8.1:+61455433 None:intergenic
AATGTTGCTGTTGAAAATGT+TGG 0.554684 8.1:+61455065 None:intergenic
ACGAGCATTGTTGTGAGCAA+CGG 0.555809 8.1:+61456072 None:intergenic
GCTGAATTTCAGAATGTTGG+AGG 0.560795 8.1:-61454182 MS.gene063911:CDS
CTTAGTCCTCTTGTTCAACC+GGG 0.562802 8.1:-61455941 MS.gene063911:CDS
CCCTTTCTTCACATAGATCA+CGG 0.563259 8.1:+61455199 None:intergenic
GTCTTTCGCGACAACAATGT+CGG 0.565235 8.1:+61455289 None:intergenic
GTTGTCGCGAAAGACGGGAG+TGG 0.565313 8.1:-61455281 MS.gene063911:CDS
TTGTGATGTTACATTGTACA+AGG 0.567974 8.1:-61455981 MS.gene063911:CDS
CGACAATTTGAACAACTCCT+CGG 0.568069 8.1:+61455904 None:intergenic
TGATTCGTCGACTTTACCAT+AGG 0.568245 8.1:+61456201 None:intergenic
CATCATCACATGGCTTAACA+AGG 0.570892 8.1:-61455432 MS.gene063911:CDS
GCATTAACTGCAGTTTCGAA+AGG 0.570948 8.1:-61455872 MS.gene063911:CDS
TTACCAAGACAACCTATGCC+AGG 0.573972 8.1:-61454443 MS.gene063911:CDS
ATAGGCTTTAAGAACACAGC+TGG 0.577241 8.1:-61454659 MS.gene063911:CDS
CCGGTTGAACAAGAGGACTA+AGG 0.579986 8.1:+61455942 None:intergenic
TTATTATGAGAATGTTAGGG+TGG 0.582078 8.1:-61455174 MS.gene063911:CDS
TCGGGTCGTATTTGCTGACC+CGG 0.587545 8.1:+61455923 None:intergenic
GATACCAATGAGGACAATTG+TGG 0.590032 8.1:+61456117 None:intergenic
TACCGCGAATGCAACATCTA+CGG 0.593477 8.1:-61454521 MS.gene063911:CDS
GTTCAGTATTTATCTGACCA+TGG 0.597410 8.1:-61455848 MS.gene063911:CDS
TAAATGTTGCACCAGAAGCA+GGG 0.599444 8.1:+61454051 None:intergenic
ATAGCTCTTTGTCTTATGGA+GGG 0.602541 8.1:-61455730 MS.gene063911:CDS
CCATGAAGAATAGGGTCAAG+TGG 0.608609 8.1:-61454151 MS.gene063911:CDS
AAGAATAGGGTCAAGTGGAA+GGG 0.609533 8.1:-61454146 MS.gene063911:CDS
AAGCCATGTTCGAAGTTCGA+AGG 0.613588 8.1:-61456174 MS.gene063911:CDS
GAATTTCAGAATGTTGGAGG+TGG 0.614077 8.1:-61454179 MS.gene063911:CDS
ACAGTCTTGCCATCATCACA+TGG 0.638538 8.1:-61455442 MS.gene063911:CDS
TTGGGTTGGAGATTCGGCGC+CGG 0.641411 8.1:-61454219 MS.gene063911:CDS
GAATTTGGATAATAGAACAA+AGG 0.642422 8.1:-61455804 MS.gene063911:CDS
ATAGGCCTTAAAGGAAGACA+TGG 0.648840 8.1:+61456219 None:intergenic
ACTGATCCTAACATGAACAC+AGG 0.655015 8.1:-61454389 MS.gene063911:CDS
GGCGCCGAATCTCCAACCCA+AGG 0.664024 8.1:+61454221 None:intergenic
GGGTCAGCAAATACGACCCG+AGG 0.685682 8.1:-61455921 MS.gene063911:CDS
TTGGAAGAATTACTCAACAA+CGG 0.709135 8.1:-61454294 MS.gene063911:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGAAACAATAAATTAATA+TGG + chr8.1:61455524-61455543 None:intergenic 10.0%
!! ATTACTTAATTTCAATTTAA+AGG + chr8.1:61455231-61455250 None:intergenic 10.0%
!!! AATTTATTGTTTCTTTTCTA+TGG - chr8.1:61455528-61455547 MS.gene063911:CDS 15.0%
!!! TTTTATGATTATTTCTTTGT+TGG + chr8.1:61455469-61455488 None:intergenic 15.0%
!! AAATGGAATGTTATGATTAT+TGG - chr8.1:61455111-61455130 MS.gene063911:CDS 20.0%
!! AGTTCTGTTAAAACATATTT+AGG - chr8.1:61455931-61455950 MS.gene063911:CDS 20.0%
!! GATTTATTATGAGAATGTTA+GGG - chr8.1:61455077-61455096 MS.gene063911:CDS 20.0%
! AATGTTATGATTATTGGTGA+TGG - chr8.1:61455117-61455136 MS.gene063911:CDS 25.0%
! ATGAATGTTACTATTGTTTC+TGG - chr8.1:61455141-61455160 MS.gene063911:CDS 25.0%
! CATTGTTTATTCATCTATAC+AGG - chr8.1:61454688-61454707 MS.gene063911:CDS 25.0%
! GAATTTGGATAATAGAACAA+AGG - chr8.1:61454450-61454469 MS.gene063911:CDS 25.0%
! GGATTTATTATGAGAATGTT+AGG - chr8.1:61455076-61455095 MS.gene063911:CDS 25.0%
! TAAGGATTTGAACTTGAATT+TGG - chr8.1:61454435-61454454 MS.gene063911:CDS 25.0%
! TCAGCATAAAATATAGTATC+CGG + chr8.1:61456057-61456076 None:intergenic 25.0%
! TTTAATATTCAGCTGTGTTT+GGG - chr8.1:61455551-61455570 MS.gene063911:intron 25.0%
!! ATCAATTGTAAGGATCTTTT+GGG - chr8.1:61454481-61454500 MS.gene063911:CDS 25.0%
!! ATTTTATTAGTCCAAATGGA+TGG - chr8.1:61456007-61456026 MS.gene063911:CDS 25.0%
!! GGAAATTTTATTAGTCCAAA+TGG - chr8.1:61456003-61456022 MS.gene063911:CDS 25.0%
!! GGAAGTCTTAATTTTATAGA+TGG - chr8.1:61455162-61455181 MS.gene063911:CDS 25.0%
!!! ATCTCTTTCAAGTTCAATTA+AGG - chr8.1:61454246-61454265 MS.gene063911:CDS 25.0%
!!! TCCTATTGTCACTTTTATTA+GGG + chr8.1:61455035-61455054 None:intergenic 25.0%
AAACATATTTAGGTAGACCT+TGG - chr8.1:61455941-61455960 MS.gene063911:CDS 30.0%
AATTTCATTGCAAGAGACAT+AGG - chr8.1:61455577-61455596 MS.gene063911:intron 30.0%
AGCTCATAAGTTTACAGTTA+AGG - chr8.1:61456155-61456174 MS.gene063911:CDS 30.0%
ATCAAAATGAAGAACCAAAC+TGG - chr8.1:61454887-61454906 MS.gene063911:intron 30.0%
GTATAAATTAACTTATGCCG+AGG + chr8.1:61454657-61454676 None:intergenic 30.0%
GTTTAATATTCAGCTGTGTT+TGG - chr8.1:61455550-61455569 MS.gene063911:intron 30.0%
TATGCTGAATTTCAGAATGT+TGG - chr8.1:61456069-61456088 MS.gene063911:CDS 30.0%
TCAAATCCTTAAAAGTTCCA+TGG + chr8.1:61454426-61454445 None:intergenic 30.0%
TCCCTAATAAAAGTGACAAT+AGG - chr8.1:61455031-61455050 MS.gene063911:intron 30.0%
TTATTATGAGAATGTTAGGG+TGG - chr8.1:61455080-61455099 MS.gene063911:CDS 30.0%
TTGAATAGCTCTTTGTCTTA+TGG - chr8.1:61454520-61454539 MS.gene063911:CDS 30.0%
TTGGAAGAATTACTCAACAA+CGG - chr8.1:61455960-61455979 MS.gene063911:CDS 30.0%
TTGTGATGTTACATTGTACA+AGG - chr8.1:61454273-61454292 MS.gene063911:CDS 30.0%
! AATGTTGCTGTTGAAAATGT+TGG + chr8.1:61455192-61455211 None:intergenic 30.0%
! ACAGTTAAGGCTTTTCTTAA+TGG - chr8.1:61456168-61456187 MS.gene063911:CDS 30.0%
! ACATTTTCAACAGCAACATT+TGG - chr8.1:61455192-61455211 MS.gene063911:CDS 30.0%
! AGCTATAAATTTTGTGCATC+TGG + chr8.1:61455403-61455422 None:intergenic 30.0%
! AGTTTTCTACAAATGTTCGA+TGG - chr8.1:61455666-61455685 MS.gene063911:intron 30.0%
! CTTTTCTTAATGGAGATAGA+TGG - chr8.1:61456178-61456197 MS.gene063911:CDS 30.0%
! GATCAATTGTAAGGATCTTT+TGG - chr8.1:61454480-61454499 MS.gene063911:CDS 30.0%
! GCTATAAATTTTGTGCATCT+GGG + chr8.1:61455402-61455421 None:intergenic 30.0%
! GTCTTCTTTTCTTGATGTTT+TGG - chr8.1:61454549-61454568 MS.gene063911:CDS 30.0%
!! TTCTTTTCTTGATGTTTTGG+AGG - chr8.1:61454552-61454571 MS.gene063911:CDS 30.0%
!!! GTCCTATTGTCACTTTTATT+AGG + chr8.1:61455036-61455055 None:intergenic 30.0%
!!! TTCTGTAGAGTTTTTGAGAT+TGG + chr8.1:61454782-61454801 None:intergenic 30.0%
AATAGCTCTTTGTCTTATGG+AGG - chr8.1:61454523-61454542 MS.gene063911:CDS 35.0%
ACAGAACTCAGATTTCCAAA+AGG + chr8.1:61455919-61455938 None:intergenic 35.0%
ATAGCTCTTTGTCTTATGGA+GGG - chr8.1:61454524-61454543 MS.gene063911:CDS 35.0%
GAAATTCCTGTGTTCATGTT+AGG + chr8.1:61455874-61455893 None:intergenic 35.0%
GTATGCAATATATTCGTCCT+CGG - chr8.1:61454637-61454656 MS.gene063911:CDS 35.0%
GTGTTTATGCAATCGAACTT+GGG - chr8.1:61455982-61456001 MS.gene063911:CDS 35.0%
GTTCAGTATTTATCTGACCA+TGG - chr8.1:61454406-61454425 MS.gene063911:CDS 35.0%
GTTGTTGAGTAATTCTTCCA+AGG + chr8.1:61455961-61455980 None:intergenic 35.0%
TCTTTATGATGAAGCCAGTT+TGG + chr8.1:61454904-61454923 None:intergenic 35.0%
TTAGGGTGGAAAAGACTAAA+TGG - chr8.1:61455094-61455113 MS.gene063911:CDS 35.0%
! ATCTGACCATGGAACTTTTA+AGG - chr8.1:61454417-61454436 MS.gene063911:CDS 35.0%
! GGAAGCTTTGATCAATTGTA+AGG - chr8.1:61454471-61454490 MS.gene063911:CDS 35.0%
!! GGAACAGTCGATTTCATTTT+CGG - chr8.1:61455754-61455773 MS.gene063911:CDS 35.0%
!! GTTCAAATTGTCGATTCAAG+TGG - chr8.1:61454360-61454379 MS.gene063911:CDS 35.0%
!! GTTGCAGTTTTGTAATACGA+CGG + chr8.1:61455788-61455807 None:intergenic 35.0%
AACCATCAATGCATGTTTGC+TGG + chr8.1:61454720-61454739 None:intergenic 40.0%
AAGAATAGGGTCAAGTGGAA+GGG - chr8.1:61456108-61456127 MS.gene063911:CDS 40.0%
AAGCACTCAACCAAGTTTCA+AGG + chr8.1:61454579-61454598 None:intergenic 40.0%
ACCGTGATCTATGTGAAGAA+AGG - chr8.1:61455054-61455073 MS.gene063911:intron 40.0%
ACTGATCCTAACATGAACAC+AGG - chr8.1:61455865-61455884 MS.gene063911:CDS 40.0%
AGCTTAAAATCTAGACCGTG+TGG - chr8.1:61455323-61455342 MS.gene063911:CDS 40.0%
CAGAACACAATAACAGCACA+AGG - chr8.1:61455838-61455857 MS.gene063911:CDS 40.0%
CATCATCACATGGCTTAACA+AGG - chr8.1:61454822-61454841 MS.gene063911:intron 40.0%
CCCTTTCTTCACATAGATCA+CGG + chr8.1:61455058-61455077 None:intergenic 40.0%
CCGTGATCTATGTGAAGAAA+GGG - chr8.1:61455055-61455074 MS.gene063911:intron 40.0%
CGACAATTTGAACAACTCCT+CGG + chr8.1:61454353-61454372 None:intergenic 40.0%
CTGCAAAAACGATACCAATG+AGG + chr8.1:61454150-61454169 None:intergenic 40.0%
CTTGTTAAGCCATGTGATGA+TGG + chr8.1:61454824-61454843 None:intergenic 40.0%
GAATTTCAGAATGTTGGAGG+TGG - chr8.1:61456075-61456094 MS.gene063911:CDS 40.0%
GACAATTTGAACAACTCCTC+GGG + chr8.1:61454352-61454371 None:intergenic 40.0%
GACTTTACCATAGGCCTTAA+AGG + chr8.1:61454047-61454066 None:intergenic 40.0%
GATACCAATGAGGACAATTG+TGG + chr8.1:61454140-61454159 None:intergenic 40.0%
GCATTAACTGCAGTTTCGAA+AGG - chr8.1:61454382-61454401 MS.gene063911:CDS 40.0%
GCTGAATTTCAGAATGTTGG+AGG - chr8.1:61456072-61456091 MS.gene063911:CDS 40.0%
GGTGTTTATGCAATCGAACT+TGG - chr8.1:61455981-61456000 MS.gene063911:CDS 40.0%
TAAAATCTAGACCGTGTGGT+CGG - chr8.1:61455327-61455346 MS.gene063911:CDS 40.0%
TAAATGTTGCACCAGAAGCA+GGG + chr8.1:61456206-61456225 None:intergenic 40.0%
TACATGCAGACTGACATATC+AGG + chr8.1:61455354-61455373 None:intergenic 40.0%
TACCAGCAAACATGCATTGA+TGG - chr8.1:61454715-61454734 MS.gene063911:intron 40.0%
TCCAAATGGATGGTTACCTT+GGG - chr8.1:61456017-61456036 MS.gene063911:CDS 40.0%
TGGTGCTTCCATGAAGAATA+GGG - chr8.1:61456095-61456114 MS.gene063911:CDS 40.0%
TTAAATGTTGCACCAGAAGC+AGG + chr8.1:61456207-61456226 None:intergenic 40.0%
TTGTCCACAATTGTCCTCAT+TGG - chr8.1:61454133-61454152 MS.gene063911:CDS 40.0%
TTTCCTTCGAACTTCGAACA+TGG + chr8.1:61454086-61454105 None:intergenic 40.0%
! AATTGCAATGTGTCGCCTTT+TGG - chr8.1:61455901-61455920 MS.gene063911:CDS 40.0%
! CACGGTCTAGATTTTAAGCT+CGG + chr8.1:61455323-61455342 None:intergenic 40.0%
! GTCGGCTTTCTTCTTCAAAT+CGG + chr8.1:61454950-61454969 None:intergenic 40.0%
! TTGTCATTAGTGCTACTGCT+TGG + chr8.1:61455633-61455652 None:intergenic 40.0%
!! ATAGGCTTTAAGAACACAGC+TGG - chr8.1:61455595-61455614 MS.gene063911:intron 40.0%
!! ATGTCTTCCTTTAAGGCCTA+TGG - chr8.1:61454037-61454056 MS.gene063911:CDS 40.0%
!! CATTGGTATCGTTTTTGCAG+CGG - chr8.1:61454150-61454169 MS.gene063911:CDS 40.0%
!! TGATTCGTCGACTTTACCAT+AGG + chr8.1:61454056-61454075 None:intergenic 40.0%
!! TTTTGGAGGACCTTGAAACT+TGG - chr8.1:61454566-61454585 MS.gene063911:CDS 40.0%
AAGCCATGTTCGAAGTTCGA+AGG - chr8.1:61454080-61454099 MS.gene063911:CDS 45.0%
AATGGATGGTTACCTTGGGT+TGG - chr8.1:61456021-61456040 MS.gene063911:CDS 45.0%
ACAGTCTTGCCATCATCACA+TGG - chr8.1:61454812-61454831 MS.gene063911:intron 45.0%
ACATTGTTGTCGCGAAAGAC+GGG - chr8.1:61454968-61454987 MS.gene063911:intron 45.0%
ACCCAAGGTAACCATCCATT+TGG + chr8.1:61456021-61456040 None:intergenic 45.0%
CCACTTGACCCTATTCTTCA+TGG + chr8.1:61456106-61456125 None:intergenic 45.0%
CCATGAAGAATAGGGTCAAG+TGG - chr8.1:61456103-61456122 MS.gene063911:CDS 45.0%
CCTTAGTCCTCTTGTTCAAC+CGG - chr8.1:61454312-61454331 MS.gene063911:CDS 45.0%
CTTAGTCCTCTTGTTCAACC+GGG - chr8.1:61454313-61454332 MS.gene063911:CDS 45.0%
GAAACTTGGTTGAGTGCTTC+AGG - chr8.1:61454580-61454599 MS.gene063911:CDS 45.0%
GAAGAATAGGGTCAAGTGGA+AGG - chr8.1:61456107-61456126 MS.gene063911:CDS 45.0%
GACATTGTTGTCGCGAAAGA+CGG - chr8.1:61454967-61454986 MS.gene063911:intron 45.0%
GCTTAACAAGGCTGCATCTT+CGG - chr8.1:61454834-61454853 MS.gene063911:intron 45.0%
GTCCAAATGGATGGTTACCT+TGG - chr8.1:61456016-61456035 MS.gene063911:CDS 45.0%
GTCTTTCGCGACAACAATGT+CGG + chr8.1:61454968-61454987 None:intergenic 45.0%
GTGGTGCTTCCATGAAGAAT+AGG - chr8.1:61456094-61456113 MS.gene063911:CDS 45.0%
GTTATTGTGTTCTGCTGTCC+TGG + chr8.1:61455832-61455851 None:intergenic 45.0%
TACCGCGAATGCAACATCTA+CGG - chr8.1:61455733-61455752 MS.gene063911:CDS 45.0%
TCAGTCTGCATGTAGTCACA+AGG - chr8.1:61455360-61455379 MS.gene063911:CDS 45.0%
TCGAACTTCGAACATGGCTT+GGG + chr8.1:61454080-61454099 None:intergenic 45.0%
TGTTGCATTCGCGGTAGAAT+TGG + chr8.1:61455729-61455748 None:intergenic 45.0%
TTACCAAGACAACCTATGCC+AGG - chr8.1:61455811-61455830 MS.gene063911:CDS 45.0%
TTCCGTAGATGTTGCATTCG+CGG + chr8.1:61455738-61455757 None:intergenic 45.0%
TTCGAACTTCGAACATGGCT+TGG + chr8.1:61454081-61454100 None:intergenic 45.0%
! ACGAGCATTGTTGTGAGCAA+CGG + chr8.1:61454185-61454204 None:intergenic 45.0%
!! ATCGTTTTTGCAGCGGTGTT+TGG - chr8.1:61454157-61454176 MS.gene063911:CDS 45.0%
!! GTTACCTTGGGTTGGAGATT+CGG - chr8.1:61456029-61456048 MS.gene063911:CDS 45.0%
!!! GCTACTGCTTGGTGTTTTTG+TGG + chr8.1:61455622-61455641 None:intergenic 45.0%
ACATATCAGGACCGACCACA+CGG + chr8.1:61455341-61455360 None:intergenic 50.0%
CCGGTTGAACAAGAGGACTA+AGG + chr8.1:61454315-61454334 None:intergenic 50.0%
GATAGATGGATCCCTGCTTC+TGG - chr8.1:61456192-61456211 MS.gene063911:CDS 50.0%
TGTCCTGGCATAGGTTGTCT+TGG + chr8.1:61455817-61455836 None:intergenic 50.0%
GTGTTCTGCTGTCCTGGCAT+AGG + chr8.1:61455826-61455845 None:intergenic 55.0%
TGCTGACCCGGTTGAACAAG+AGG + chr8.1:61454322-61454341 None:intergenic 55.0%
TTGTCGCGAAAGACGGGAGT+GGG - chr8.1:61454974-61454993 MS.gene063911:intron 55.0%
! TCGGGTCGTATTTGCTGACC+CGG + chr8.1:61454334-61454353 None:intergenic 55.0%
GGGTCAGCAAATACGACCCG+AGG - chr8.1:61454333-61454352 MS.gene063911:CDS 60.0%
GTTGTCGCGAAAGACGGGAG+TGG - chr8.1:61454973-61454992 MS.gene063911:intron 60.0%
!! TTGGGTTGGAGATTCGGCGC+CGG - chr8.1:61456035-61456054 MS.gene063911:CDS 60.0%
! GGCGCCGAATCTCCAACCCA+AGG + chr8.1:61456036-61456055 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 61454037 61456239 61454037 ID=MS.gene063911
chr8.1 mRNA 61454037 61456239 61454037 ID=MS.gene063911.t1;Parent=MS.gene063911
chr8.1 exon 61455675 61456239 61455675 ID=MS.gene063911.t1.exon1;Parent=MS.gene063911.t1
chr8.1 CDS 61455675 61456239 61455675 ID=cds.MS.gene063911.t1;Parent=MS.gene063911.t1
chr8.1 exon 61455063 61455566 61455063 ID=MS.gene063911.t1.exon2;Parent=MS.gene063911.t1
chr8.1 CDS 61455063 61455566 61455063 ID=cds.MS.gene063911.t1;Parent=MS.gene063911.t1
chr8.1 exon 61454037 61454713 61454037 ID=MS.gene063911.t1.exon3;Parent=MS.gene063911.t1
chr8.1 CDS 61454037 61454713 61454037 ID=cds.MS.gene063911.t1;Parent=MS.gene063911.t1
Gene Sequence

>MS.gene063911

ATGTCTTCCTTTAAGGCCTATGGTAAAGTCGACGAATCAGCCCAAGCCATGTTCGAAGTTCGAAGGAAATCACGAAAGCGAATAACTATCATAAGCTTGTCCACAATTGTCCTCATTGGTATCGTTTTTGCAGCGGTGTTTGGAACCGTTGCTCACAACAATGCTCGTAATGATGACAACAACAACAACAATAATGATAACGACGATCAATCTCTTTCAAGTTCAATTAAGGCTGTTTGTGATGTTACATTGTACAAGGATTCATGTGAAAGCAGCCTTAGTCCTCTTGTTCAACCGGGTCAGCAAATACGACCCGAGGAGTTGTTCAAATTGTCGATTCAAGTGGCATTAACTGCAGTTTCGAAAGGTGTTCAGTATTTATCTGACCATGGAACTTTTAAGGATTTGAACTTGAATTTGGATAATAGAACAAAGGAAGCTTTGATCAATTGTAAGGATCTTTTGGGTCTTGCTGTTGATCATTTGAATAGCTCTTTGTCTTATGGAGGGAAGTCTTCTTTTCTTGATGTTTTGGAGGACCTTGAAACTTGGTTGAGTGCTTCAGGTAAACTTTATAATTAAGCATTTATATACTTTTTTGTATGCAATATATTCGTCCTCGGCATAAGTTAATTTATACTTCATAACAAGCATTGTTTATTCATCTATACAGGTACTTACCAGCAAACATGCATTGATGGTTTAGAACAAGCAGAAGAAGCATTAAAGTCTAGTATTACAACCAATCTCAAAAACTCTACAGAATTTACGAGCAACAGTCTTGCCATCATCACATGGCTTAACAAGGCTGCATCTTCGGTGAACTTTAGACGCTTACTGAGTTTATCGCATCAAAATGAAGAACCAAACTGGCTTCATCATAAAGACAGAAAGTTACTTCAAAAAGACACCGATTTGAAGAAGAAAGCCGACATTGTTGTCGCGAAAGACGGGAGTGGGAAATACAAAACAATTTCAGCTGCATTGAAACATGTCCCTAATAAAAGTGACAATAGGACCGTGATCTATGTGAAGAAAGGGATTTATTATGAGAATGTTAGGGTGGAAAAGACTAAATGGAATGTTATGATTATTGGTGATGGAATGAATGTTACTATTGTTTCTGGAAGTCTTAATTTTATAGATGGCACACCAACATTTTCAACAGCAACATTTGGTATGTCAAACTTTCCTTTAAATTGAAATTAAGTAATTATATACAACATTTTTTCGATTCAGATTTCAATGCTGATTTTTTATATTTTTTTTTGAGAATTAAAACACCGAGCTTAAAATCTAGACCGTGTGGTCGGTCCTGATATGTCAGTCTGCATGTAGTCACAAGGTTGTGAATGCACACAACCCAGATGCACAAAATTTATAGCTATTCTATTTCTTTTTGCGTATCAATTTGAAAACTTCTTATTCTCCAACAAAGAAATAATCATAAAAAATCTTTTAACATGATTTATAAAACTGTCTAACCATATTAATTTATTGTTTCTTTTCTATGGTTTAATATTCAGCTGTGTTTGGGAGAAATTTCATTGCAAGAGACATAGGCTTTAAGAACACAGCTGGTCCACAAAAACACCAAGCAGTAGCACTAATGACAAGTGCAGATCAAGCAGTTTTCTACAAATGTTCGATGGATGCATATCAAGACACTCTCTACGCTCACTCAAATCGCCAATTCTACCGCGAATGCAACATCTACGGAACAGTCGATTTCATTTTCGGAAACTCAGCCGTCGTATTACAAAACTGCAACATTTTACCAAGACAACCTATGCCAGGACAGCAGAACACAATAACAGCACAAGGCAAAACTGATCCTAACATGAACACAGGAATTTCAATTCAAAATTGCAATGTGTCGCCTTTTGGAAATCTGAGTTCTGTTAAAACATATTTAGGTAGACCTTGGAAGAATTACTCAACAACGGTGTTTATGCAATCGAACTTGGGAAATTTTATTAGTCCAAATGGATGGTTACCTTGGGTTGGAGATTCGGCGCCGGATACTATATTTTATGCTGAATTTCAGAATGTTGGAGGTGGTGCTTCCATGAAGAATAGGGTCAAGTGGAAGGGTTTGAAAACTATTACAAGTAAACAAGCTCATAAGTTTACAGTTAAGGCTTTTCTTAATGGAGATAGATGGATCCCTGCTTCTGGTGCAACATTTAAATCTTCTCTGTAA
Protein sequence

>MS.gene063911.t1

MSSFKAYGKVDESAQAMFEVRRKSRKRITIISLSTIVLIGIVFAAVFGTVAHNNARNDDNNNNNNDNDDQSLSSSIKAVCDVTLYKDSCESSLSPLVQPGQQIRPEELFKLSIQVALTAVSKGVQYLSDHGTFKDLNLNLDNRTKEALINCKDLLGLAVDHLNSSLSYGGKSSFLDVLEDLETWLSASGTYQQTCIDGLEQAEEALKSSITTNLKNSTEFTSNSLAIITWLNKAASSVNFRRLLSLSHQNEEPNWLHHKDRKLLQKDTDLKKKADIVVAKDGSGKYKTISAALKHVPNKSDNRTVIYVKKGIYYENVRVEKTKWNVMIIGDGMNVTIVSGSLNFIDGTPTFSTATFAVFGRNFIARDIGFKNTAGPQKHQAVALMTSADQAVFYKCSMDAYQDTLYAHSNRQFYRECNIYGTVDFIFGNSAVVLQNCNILPRQPMPGQQNTITAQGKTDPNMNTGISIQNCNVSPFGNLSSVKTYLGRPWKNYSTTVFMQSNLGNFISPNGWLPWVGDSAPDTIFYAEFQNVGGGASMKNRVKWKGLKTITSKQAHKFTVKAFLNGDRWIPASGATFKSSL