AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene064158

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064158.t1 MTR_4g123750 97.106 311 9 0 1 311 1 311 0.0 633
MS.gene064158.t1 MTR_8g006190 39.310 290 157 4 35 307 5 292 1.85e-64 209
MS.gene064158.t1 MTR_8g006190 23.051 295 192 9 28 307 165 439 2.11e-11 64.7
MS.gene064158.t1 MTR_8g006190 37.956 274 151 4 51 307 13 284 9.05e-55 184
MS.gene064158.t1 MTR_8g006190 23.051 295 192 9 28 307 157 431 1.98e-11 64.7
MS.gene064158.t1 MTR_7g096550 94.000 50 3 0 1 50 1 50 3.83e-27 100
MS.gene064158.t1 MTR_5g091450 35.036 137 80 4 16 152 30 157 4.91e-19 88.2
MS.gene064158.t1 MTR_5g091450 30.000 130 88 2 174 302 31 158 2.06e-12 68.2
MS.gene064158.t1 MTR_5g086700 32.414 145 88 4 12 156 54 188 4.38e-17 82.4
MS.gene064158.t1 MTR_5g086700 31.915 141 92 2 169 308 54 191 1.38e-16 80.9
MS.gene064158.t1 MTR_5g086700 32.414 145 88 4 12 156 54 188 4.67e-17 82.4
MS.gene064158.t1 MTR_5g086700 31.915 141 92 2 169 308 54 191 1.43e-16 80.9
MS.gene064158.t1 MTR_3g079840 32.624 141 91 2 169 308 49 186 3.16e-16 79.7
MS.gene064158.t1 MTR_3g079840 31.138 167 95 6 9 165 40 196 3.50e-16 79.7
MS.gene064158.t1 MTR_3g079840 32.624 141 91 2 169 308 50 187 3.32e-16 79.7
MS.gene064158.t1 MTR_3g079840 31.138 167 95 6 9 165 41 197 3.67e-16 79.7
MS.gene064158.t1 MTR_5g091180 31.915 141 83 5 14 152 41 170 1.01e-15 78.2
MS.gene064158.t1 MTR_5g091180 30.370 135 89 4 170 302 40 171 3.93e-12 67.4
MS.gene064158.t1 MTR_4g123790 73.810 42 11 0 164 205 12 53 1.83e-15 71.2
MS.gene064158.t1 MTR_1g042160 23.871 155 110 3 20 174 14 160 7.20e-15 74.3
MS.gene064158.t1 MTR_1g041240 28.082 146 93 4 13 157 51 185 1.18e-14 75.1
MS.gene064158.t1 MTR_1g041240 26.486 185 126 5 129 308 8 187 3.30e-13 70.9
MS.gene064158.t1 MTR_1g041240 28.082 146 93 4 13 157 50 184 1.23e-14 75.1
MS.gene064158.t1 MTR_1g041240 28.671 143 97 3 167 308 48 186 1.25e-12 68.9
MS.gene064158.t1 MTR_1g041240 28.082 146 93 4 13 157 52 186 1.25e-14 75.1
MS.gene064158.t1 MTR_1g041240 25.946 185 128 4 129 308 8 188 4.51e-13 70.1
MS.gene064158.t1 MTR_5g091550 33.553 152 91 5 40 190 37 179 2.20e-13 70.9
MS.gene064158.t1 MTR_5g091550 29.921 127 85 2 177 302 20 143 1.90e-11 65.1
MS.gene064158.t1 MTR_5g091550 31.788 151 90 5 40 190 37 174 3.01e-13 70.5
MS.gene064158.t1 MTR_5g091550 29.921 127 85 2 177 302 20 143 2.05e-11 64.7
MS.gene064158.t1 MTR_3g106700 19.580 286 186 8 39 301 432 696 1.90e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064158.t1 AT3G17380 52.459 305 142 1 6 310 8 309 5.02e-112 327
MS.gene064158.t1 AT5G26280 32.353 306 201 4 7 311 49 349 2.64e-49 167
MS.gene064158.t1 AT5G26280 32.353 306 201 4 7 311 68 368 6.36e-49 167
MS.gene064158.t1 AT5G26260 32.895 304 198 4 9 311 52 350 1.33e-48 166
MS.gene064158.t1 AT5G26290 32.026 306 197 4 6 311 41 335 1.03e-44 155
MS.gene064158.t1 AT4G09780 34.641 306 183 10 8 309 53 345 5.87e-43 152
MS.gene064158.t1 AT2G04170 31.864 295 195 4 17 310 7 296 8.46e-43 149
MS.gene064158.t1 AT2G04170 31.864 295 195 4 17 310 7 296 8.46e-43 149
MS.gene064158.t1 AT5G26300 30.392 306 206 5 7 311 49 348 1.30e-42 150
MS.gene064158.t1 AT4G09770 33.333 306 186 10 8 309 26 317 3.97e-42 148
MS.gene064158.t1 AT4G09770 33.333 303 184 10 11 309 3 291 5.45e-42 147
MS.gene064158.t1 AT4G09770 33.333 303 184 10 11 309 3 291 5.45e-42 147
MS.gene064158.t1 AT2G04170 31.864 295 195 4 17 310 89 378 5.73e-42 149
MS.gene064158.t1 AT2G04170 31.864 295 195 4 17 310 129 418 1.28e-41 149
MS.gene064158.t1 AT2G04170 31.864 295 195 4 17 310 129 418 1.28e-41 149
MS.gene064158.t1 AT4G09780 33.333 315 186 9 8 309 53 356 8.23e-41 147
MS.gene064158.t1 AT3G28220 32.404 287 183 7 17 300 80 358 1.22e-40 145
MS.gene064158.t1 AT3G20370 30.897 301 195 7 10 304 56 349 1.26e-40 145
MS.gene064158.t1 AT3G20370 30.897 301 195 7 10 304 78 371 2.31e-40 145
MS.gene064158.t1 AT3G20360 30.341 323 206 9 1 310 45 361 1.21e-39 142
MS.gene064158.t1 AT4G09770 35.060 251 148 8 8 255 26 264 2.02e-38 137
MS.gene064158.t1 AT4G09770 35.081 248 146 8 11 255 3 238 2.82e-38 136
MS.gene064158.t1 AT3G22080 28.808 302 201 7 10 305 12 305 3.18e-38 137
MS.gene064158.t1 AT5G26320 28.571 308 212 5 7 311 49 351 2.44e-37 136
MS.gene064158.t1 AT2G04170 33.884 242 155 3 14 255 126 362 6.51e-37 135
MS.gene064158.t1 AT5G26280 28.431 306 190 5 7 311 49 326 1.59e-35 131
MS.gene064158.t1 AT2G04190 29.642 307 205 7 8 310 110 409 1.72e-35 132
MS.gene064158.t1 AT2G15710 30.420 286 179 7 5 287 86 354 1.06e-33 127
MS.gene064158.t1 AT4G00780 30.137 292 183 8 20 307 22 296 4.29e-32 121
MS.gene064158.t1 AT1G69650 27.273 308 184 9 8 307 13 288 8.61e-32 120
MS.gene064158.t1 AT4G01390 29.236 301 173 8 20 306 22 296 4.69e-31 118
MS.gene064158.t1 AT2G04170 37.306 193 116 3 14 206 126 313 1.51e-30 117
MS.gene064158.t1 AT1G58270 28.383 303 196 9 17 311 94 383 1.68e-29 116
MS.gene064158.t1 AT3G22085 28.996 269 164 7 6 272 7 250 1.82e-29 114
MS.gene064158.t1 AT1G65150 31.667 240 147 7 36 272 32 257 1.08e-28 112
MS.gene064158.t1 AT1G65150 31.667 240 147 7 36 272 32 257 1.08e-28 112
MS.gene064158.t1 AT3G20380 24.830 294 215 3 8 301 77 364 1.17e-28 113
MS.gene064158.t1 AT2G32870 25.000 320 213 7 5 310 110 416 6.14e-26 107
MS.gene064158.t1 AT1G65370 28.821 229 147 6 78 304 7 221 6.19e-25 100
MS.gene064158.t1 AT2G32880 25.155 322 212 10 5 310 10 318 8.45e-24 99.4
MS.gene064158.t1 AT1G69660 26.872 227 159 4 82 307 5 225 2.27e-23 96.3
MS.gene064158.t1 AT2G32880 26.336 262 169 8 5 255 10 258 5.29e-21 91.3
MS.gene064158.t1 AT2G32870 26.154 260 170 6 5 255 110 356 4.92e-20 89.7
MS.gene064158.t1 AT3G46190 25.510 294 178 7 14 304 29 284 1.72e-19 87.0
MS.gene064158.t1 AT3G46190 25.510 294 178 7 14 304 49 304 2.20e-19 87.0
MS.gene064158.t1 AT1G65050 34.545 110 68 2 36 145 32 137 6.89e-18 79.7
MS.gene064158.t1 AT1G65050 34.545 110 68 2 36 145 32 137 6.89e-18 79.7
MS.gene064158.t1 AT5G06600 31.250 160 94 5 1 154 32 181 6.54e-16 79.0
MS.gene064158.t1 AT5G06600 32.143 140 91 2 170 308 50 186 6.55e-15 75.9
MS.gene064158.t1 AT5G06600 31.250 160 94 5 1 154 31 180 6.87e-16 79.0
MS.gene064158.t1 AT5G06600 32.143 140 91 2 170 308 49 185 5.62e-15 76.3
MS.gene064158.t1 AT5G06600 31.250 160 94 5 1 154 32 181 7.13e-16 79.0
MS.gene064158.t1 AT5G06600 32.143 140 91 2 170 308 50 186 5.78e-15 75.9
MS.gene064158.t1 AT3G11910 31.008 129 82 3 26 154 58 179 1.15e-15 78.2
MS.gene064158.t1 AT3G11910 31.429 140 92 2 170 308 48 184 1.63e-14 74.7
MS.gene064158.t1 AT3G11910 31.008 129 82 3 26 154 58 179 1.15e-15 78.2
MS.gene064158.t1 AT3G11910 31.429 140 92 2 170 308 48 184 1.63e-14 74.7
MS.gene064158.t1 AT3G58340 33.103 145 89 3 169 310 1 140 1.17e-15 76.6
MS.gene064158.t1 AT3G11910 31.008 129 82 3 26 154 59 180 1.17e-15 78.2
MS.gene064158.t1 AT3G11910 31.429 140 92 2 170 308 49 185 1.69e-14 74.7
MS.gene064158.t1 AT3G11910 31.008 129 82 3 26 154 59 180 1.17e-15 78.2
MS.gene064158.t1 AT3G11910 31.429 140 92 2 170 308 49 185 1.69e-14 74.7
MS.gene064158.t1 AT3G58340 33.103 145 89 3 169 310 1 140 1.56e-15 76.3
MS.gene064158.t1 AT3G58210 32.168 143 95 2 169 310 1 142 1.28e-14 73.6
MS.gene064158.t1 AT3G58210 32.576 132 79 4 24 154 13 135 7.16e-12 65.5
MS.gene064158.t1 AT3G58370 32.192 146 94 2 168 310 1 144 6.90e-13 68.6
MS.gene064158.t1 AT3G58370 27.941 204 134 5 24 227 14 204 7.37e-11 62.4
MS.gene064158.t1 AT1G31390 32.540 126 80 2 169 291 1 124 7.29e-13 67.8
MS.gene064158.t1 AT3G58410 31.618 136 88 2 176 311 8 138 1.52e-12 67.4
MS.gene064158.t1 AT3G58360 31.724 145 91 3 169 310 1 140 1.71e-12 67.4
MS.gene064158.t1 AT2G05420 32.374 139 87 3 178 310 11 148 1.11e-11 64.7
MS.gene064158.t1 AT1G31400 31.690 142 89 3 169 307 1 137 3.17e-11 63.2
MS.gene064158.t1 AT3G58250 30.935 139 90 3 176 311 10 145 9.53e-11 62.4

Find 70 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTGTCTGTGCATATATTT+TGG 0.140548 8.4:+11058007 None:intergenic
GTCTACTTGCAGCTAATTAT+TGG 0.237931 8.4:-11057776 MS.gene064158:intron
GATGTTGAGGTTACAGTTCT+TGG 0.266368 8.4:-11057651 MS.gene064158:CDS
TTTAATAGATGATGTGAAAA+AGG 0.278698 8.4:-11058528 MS.gene064158:intron
TCCTCTCAAGGATTTCAATC+TGG 0.293183 8.4:-11058456 MS.gene064158:CDS
AGCAATCCAAGCATCAGTTT+TGG 0.342021 8.4:-11057965 MS.gene064158:CDS
AATCTCCCATACATGCTTGT+AGG 0.374747 8.4:+11058319 None:intergenic
CCCTCCCCTACAAGCATGTA+TGG 0.374887 8.4:-11058325 MS.gene064158:CDS
CTGGATGATATATGTGCATT+TGG 0.382581 8.4:-11058416 MS.gene064158:CDS
GATTAAAGACTTCTCAAAGT+TGG 0.382721 8.4:-11058300 MS.gene064158:CDS
GAGGAACAAACCGTCATCTT+TGG 0.387041 8.4:+11059456 None:intergenic
CTGTTGGGTTAGCTAAAGTT+AGG 0.387750 8.4:+11058045 None:intergenic
ATTACTGCACAAAATAAATC+TGG 0.389938 8.4:+11057722 None:intergenic
TGTATATTGTATACCATTTG+TGG 0.403396 8.4:+11058941 None:intergenic
AGACTTCTCAAAGTTGGATT+CGG 0.407331 8.4:-11058294 MS.gene064158:CDS
GGATCATATTTCACTATACT+TGG 0.407706 8.4:-11058782 MS.gene064158:CDS
TTGTAGTTTCCAACATTGAA+TGG 0.413486 8.4:+11058254 None:intergenic
TTCACAGAATGAAGGTTGAA+TGG 0.418718 8.4:-11058496 MS.gene064158:CDS
TTCTATAGAGAGATATGAAT+CGG 0.423671 8.4:-11058983 MS.gene064158:CDS
TCACAGAATGAAGGTTGAAT+GGG 0.425550 8.4:-11058495 MS.gene064158:CDS
CCTCTCAAGGATTTCAATCT+GGG 0.426272 8.4:-11058455 MS.gene064158:CDS
GAGCAGAAGTCTTTGTTTGT+AGG 0.431985 8.4:-11058394 MS.gene064158:CDS
ACCTTTCCAAAACTGATGCT+TGG 0.443667 8.4:+11057959 None:intergenic
ATCTCCCATACATGCTTGTA+GGG 0.444436 8.4:+11058320 None:intergenic
AGCAGAAGTCTTTGTTTGTA+GGG 0.457138 8.4:-11058393 MS.gene064158:CDS
GTAAAGTACCTTGGAGAACT+AGG 0.458919 8.4:+11058671 None:intergenic
GATTTCAATCTGGGCTCTAA+AGG 0.459503 8.4:-11058446 MS.gene064158:CDS
AAGCTCACTTCATCCTGGTT+GGG 0.466058 8.4:-11058746 MS.gene064158:CDS
TAATTTCACCAGTCAATACC+TGG 0.466177 8.4:-11057703 MS.gene064158:CDS
TATTTCACTATACTTGGCAT+TGG 0.479251 8.4:-11058776 MS.gene064158:CDS
TGATCAATTTATTCCTCTCA+AGG 0.485622 8.4:-11058468 MS.gene064158:CDS
GGGCTCTAAAGGTTATCTAC+TGG 0.485976 8.4:-11058435 MS.gene064158:CDS
CAAGCTCACTTCATCCTGGT+TGG 0.493535 8.4:-11058747 MS.gene064158:CDS
TCTATAGAGAGATATGAATC+GGG 0.505763 8.4:-11058982 MS.gene064158:CDS
AAACTGTATCCCAAAGGAAA+AGG 0.507112 8.4:-11058098 MS.gene064158:CDS
ACCGTCATCTTTGGTATCCA+TGG 0.513134 8.4:+11059465 None:intergenic
TTGAAGCTGGAGGCCACAAA+TGG 0.515480 8.4:-11058954 MS.gene064158:intron
AGAATCTTTGATCATGATGA+AGG 0.521528 8.4:-11058351 MS.gene064158:CDS
GACTCTAAGCCATTCAATGT+TGG 0.526449 8.4:-11058263 MS.gene064158:CDS
CATTTGCTTTGTAGATGTTG+AGG 0.531213 8.4:-11057664 MS.gene064158:CDS
AGGGAAAACTACACAGGTAA+AGG 0.536644 8.4:-11058374 MS.gene064158:CDS
CAAAGGAAAAGGTACTGACT+CGG 0.538845 8.4:-11058087 MS.gene064158:CDS
CCCATACATGCTTGTAGGGG+AGG 0.542077 8.4:+11058324 None:intergenic
GAATCGGGGAAGTTTGAAGC+TGG 0.551406 8.4:-11058967 MS.gene064158:CDS
TAGATACCCCAGGTATTGAC+TGG 0.568960 8.4:+11057695 None:intergenic
AAACAAAAGCAAGAATGTAA+AGG 0.570463 8.4:-11058803 MS.gene064158:CDS
GAATCAAGCTCACTTCATCC+TGG 0.576113 8.4:-11058751 MS.gene064158:CDS
CACAGAATGAAGGTTGAATG+GGG 0.578801 8.4:-11058494 MS.gene064158:CDS
CCATACATGCTTGTAGGGGA+GGG 0.581374 8.4:+11058325 None:intergenic
AAAGGAAAAGGTACTGACTC+GGG 0.585829 8.4:-11058086 MS.gene064158:CDS
GTTTGTAGGGAAAACTACAC+AGG 0.592836 8.4:-11058380 MS.gene064158:CDS
TTTACATAGATTTCCCAACC+AGG 0.601857 8.4:+11058733 None:intergenic
AATTTCACCAGTCAATACCT+GGG 0.612530 8.4:-11057702 MS.gene064158:CDS
CCTCCCCTACAAGCATGTAT+GGG 0.619373 8.4:-11058324 MS.gene064158:CDS
TGAGGTTACAGTTCTTGGAG+TGG 0.629632 8.4:-11057646 MS.gene064158:CDS
ATACCTGGGGTATCTAGTGA+AGG 0.632705 8.4:-11057688 MS.gene064158:CDS
CAGATAAAACTGTATCCCAA+AGG 0.633902 8.4:-11058104 MS.gene064158:CDS
TGTCCTTCACTAGATACCCC+AGG 0.633913 8.4:+11057685 None:intergenic
TCGGGGAAGTTTGAAGCTGG+AGG 0.635431 8.4:-11058964 MS.gene064158:CDS
GACAACTACCTAGTTCTCCA+AGG 0.638057 8.4:-11058679 MS.gene064158:intron
GCCATGGATACCAAAGATGA+CGG 0.641327 8.4:-11059466 MS.gene064158:intron
TCTCTATAGAATTAGTAGTG+AGG 0.653359 8.4:+11058995 None:intergenic
CCCAGATTGAAATCCTTGAG+AGG 0.660903 8.4:+11058455 None:intergenic
TGTGCCATGTTCATGACTTG+AGG 0.660953 8.4:+11057746 None:intergenic
AAGTTAGTTATGTATCCAAG+TGG 0.687329 8.4:-11058826 MS.gene064158:CDS
TCAATGTTGGAAACTACAAG+TGG 0.691657 8.4:-11058250 MS.gene064158:intron
CTATAGAGAGATATGAATCG+GGG 0.694653 8.4:-11058981 MS.gene064158:CDS
TCTCCCATACATGCTTGTAG+GGG 0.699554 8.4:+11058321 None:intergenic
AGTGCCTCAAGTCATGAACA+TGG 0.700230 8.4:-11057750 MS.gene064158:CDS
ATTTCACCAGTCAATACCTG+GGG 0.717112 8.4:-11057701 MS.gene064158:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATTAAAAAAAAAAAAAA+AGG + chr8.4:11058446-11058465 None:intergenic 0.0%
!! TAAAAAAAAAAATTAACTCA+AGG - chr8.4:11057920-11057939 MS.gene064158:intron 10.0%
!! CTTATAAAAAATAAAATTGC+AGG - chr8.4:11058008-11058027 MS.gene064158:CDS 15.0%
!!! AATTTCATGAATGATAATTT+TGG - chr8.4:11058197-11058216 MS.gene064158:intron 15.0%
!! AGCAATCCAAAAAAAAAATT+AGG + chr8.4:11058541-11058560 None:intergenic 20.0%
!! ATATATTTCATGGATAATGA+TGG - chr8.4:11057815-11057834 MS.gene064158:intron 20.0%
!! ATGTACAATTAATAAACCAA+TGG - chr8.4:11057752-11057771 MS.gene064158:CDS 20.0%
!! CAAAAAGTTATCATATAACT+TGG + chr8.4:11058964-11058983 None:intergenic 20.0%
!! CAATCCAAAAAAAAAATTAG+GGG + chr8.4:11058539-11058558 None:intergenic 20.0%
!! GCAATCCAAAAAAAAAATTA+GGG + chr8.4:11058540-11058559 None:intergenic 20.0%
!! TGAGTACATAATATATTTCA+TGG - chr8.4:11057805-11057824 MS.gene064158:intron 20.0%
!! TGTACAATTAATAAACCAAT+GGG - chr8.4:11057753-11057772 MS.gene064158:CDS 20.0%
!! TTTAATAGATGATGTGAAAA+AGG - chr8.4:11058564-11058583 MS.gene064158:intron 20.0%
!!! ATTTTCATTATGTAATGAAC+TGG + chr8.4:11058062-11058081 None:intergenic 20.0%
!!! TGTTTTCATAATATACTTCT+TGG - chr8.4:11057704-11057723 MS.gene064158:CDS 20.0%
!!! TTATTTAGTTTAGCAAGAAA+TGG + chr8.4:11058884-11058903 None:intergenic 20.0%
!!! TTCATGAAATTATTTTTAGC+AGG + chr8.4:11058188-11058207 None:intergenic 20.0%
! AAAAAGATTTCACAGAATGA+AGG - chr8.4:11058588-11058607 MS.gene064158:intron 25.0%
! AAACAAAAGCAAGAATGTAA+AGG - chr8.4:11058289-11058308 MS.gene064158:CDS 25.0%
! ATTACTGCACAAAATAAATC+TGG + chr8.4:11059373-11059392 None:intergenic 25.0%
! TGTATATTGTATACCATTTG+TGG + chr8.4:11058154-11058173 None:intergenic 25.0%
! TTAAACAGTTTAGTGTTTGA+CGG - chr8.4:11058512-11058531 MS.gene064158:CDS 25.0%
! TTCAAACAATACTCTTTGAA+TGG + chr8.4:11058909-11058928 None:intergenic 25.0%
! TTCTATAGAGAGATATGAAT+CGG - chr8.4:11058109-11058128 MS.gene064158:intron 25.0%
!! AAAAAAAAGGTAAAGTACCT+TGG + chr8.4:11058433-11058452 None:intergenic 25.0%
!! AATAAACCAATGGGATTTTT+GGG - chr8.4:11057762-11057781 MS.gene064158:CDS 25.0%
!! AGATCAAAATCTATTTTCGA+GGG + chr8.4:11058490-11058509 None:intergenic 25.0%
!! GTTATATGATAACTTTTTGC+AGG - chr8.4:11058965-11058984 MS.gene064158:CDS 25.0%
!! TAATAAACCAATGGGATTTT+TGG - chr8.4:11057761-11057780 MS.gene064158:CDS 25.0%
!! TATTGTCTGTGCATATATTT+TGG + chr8.4:11059088-11059107 None:intergenic 25.0%
!!! TTTGGGGAACAATTTTTTTT+TGG - chr8.4:11057779-11057798 MS.gene064158:intron 25.0%
AAAATGTGTTTATACCTCAC+AGG - chr8.4:11058243-11058262 MS.gene064158:intron 30.0%
AAGTTAGTTATGTATCCAAG+TGG - chr8.4:11058266-11058285 MS.gene064158:CDS 30.0%
AGAATCTTTGATCATGATGA+AGG - chr8.4:11058741-11058760 MS.gene064158:CDS 30.0%
CACATAGAATAACTAAGCAT+AGG + chr8.4:11057983-11058002 None:intergenic 30.0%
GATTAAAGACTTCTCAAAGT+TGG - chr8.4:11058792-11058811 MS.gene064158:CDS 30.0%
GGATCATATTTCACTATACT+TGG - chr8.4:11058310-11058329 MS.gene064158:CDS 30.0%
TATTTCACTATACTTGGCAT+TGG - chr8.4:11058316-11058335 MS.gene064158:CDS 30.0%
TCTATAGAGAGATATGAATC+GGG - chr8.4:11058110-11058129 MS.gene064158:intron 30.0%
TCTCTATAGAATTAGTAGTG+AGG + chr8.4:11058100-11058119 None:intergenic 30.0%
TGATCAATTTATTCCTCTCA+AGG - chr8.4:11058624-11058643 MS.gene064158:intron 30.0%
TTGTAGTTTCCAACATTGAA+TGG + chr8.4:11058841-11058860 None:intergenic 30.0%
! AATTCCAGATTTGTTTGAGT+AGG + chr8.4:11057957-11057976 None:intergenic 30.0%
! ATAAACCAATGGGATTTTTG+GGG - chr8.4:11057763-11057782 MS.gene064158:CDS 30.0%
! ATTCCAGATTTGTTTGAGTA+GGG + chr8.4:11057956-11057975 None:intergenic 30.0%
! CAGATCAAAATCTATTTTCG+AGG + chr8.4:11058491-11058510 None:intergenic 30.0%
! GTTACACTTTGTTGTGAAAT+TGG - chr8.4:11059163-11059182 MS.gene064158:intron 30.0%
! TTTAGTTTAGCAAGAAATGG+AGG + chr8.4:11058881-11058900 None:intergenic 30.0%
AAACTGTATCCCAAAGGAAA+AGG - chr8.4:11058994-11059013 MS.gene064158:CDS 35.0%
AATTTCACCAGTCAATACCT+GGG - chr8.4:11059390-11059409 MS.gene064158:intron 35.0%
AGACTTCTCAAAGTTGGATT+CGG - chr8.4:11058798-11058817 MS.gene064158:CDS 35.0%
CAGATAAAACTGTATCCCAA+AGG - chr8.4:11058988-11059007 MS.gene064158:CDS 35.0%
CATTTGCTTTGTAGATGTTG+AGG - chr8.4:11059428-11059447 MS.gene064158:intron 35.0%
CTAAAGATGCTACCGAATAA+AGG + chr8.4:11057667-11057686 None:intergenic 35.0%
CTATAGAGAGATATGAATCG+GGG - chr8.4:11058111-11058130 MS.gene064158:intron 35.0%
CTGGATGATATATGTGCATT+TGG - chr8.4:11058676-11058695 MS.gene064158:intron 35.0%
GTCTACTTGCAGCTAATTAT+TGG - chr8.4:11059316-11059335 MS.gene064158:intron 35.0%
TAATTTCACCAGTCAATACC+TGG - chr8.4:11059389-11059408 MS.gene064158:intron 35.0%
TACATAACTAACTTCCTGTG+AGG + chr8.4:11058260-11058279 None:intergenic 35.0%
TCAATGTTGGAAACTACAAG+TGG - chr8.4:11058842-11058861 MS.gene064158:intron 35.0%
TCACAGAATGAAGGTTGAAT+GGG - chr8.4:11058597-11058616 MS.gene064158:intron 35.0%
TTCACAGAATGAAGGTTGAA+TGG - chr8.4:11058596-11058615 MS.gene064158:intron 35.0%
TTTACATAGATTTCCCAACC+AGG + chr8.4:11058362-11058381 None:intergenic 35.0%
! ACTTTGTTGTGAAATTGGTC+AGG - chr8.4:11059168-11059187 MS.gene064158:intron 35.0%
! AGCAGAAGTCTTTGTTTGTA+GGG - chr8.4:11058699-11058718 MS.gene064158:CDS 35.0%
! GAGTATTGTTTGAACTCAAC+AGG - chr8.4:11058915-11058934 MS.gene064158:intron 35.0%
!! TTCTTGCTTTTGTTTCCACT+TGG + chr8.4:11058284-11058303 None:intergenic 35.0%
!!! CATTCAGCTGTCATTTTGTA+TGG + chr8.4:11059205-11059224 None:intergenic 35.0%
!!! CGGTCCCCTAATTTTTTTTT+TGG - chr8.4:11058532-11058551 MS.gene064158:intron 35.0%
!!! GTGCATATATTTTGGAACCT+GGG + chr8.4:11059080-11059099 None:intergenic 35.0%
!!! TGCATATATTTTGGAACCTG+GGG + chr8.4:11059079-11059098 None:intergenic 35.0%
!!! TGTGCATATATTTTGGAACC+TGG + chr8.4:11059081-11059100 None:intergenic 35.0%
AATCTCCCATACATGCTTGT+AGG + chr8.4:11058776-11058795 None:intergenic 40.0%
ACCTTTCCAAAACTGATGCT+TGG + chr8.4:11059136-11059155 None:intergenic 40.0%
AGGGAAAACTACACAGGTAA+AGG - chr8.4:11058718-11058737 MS.gene064158:CDS 40.0%
ATCTCCCATACATGCTTGTA+GGG + chr8.4:11058775-11058794 None:intergenic 40.0%
ATTTCACCAGTCAATACCTG+GGG - chr8.4:11059391-11059410 MS.gene064158:intron 40.0%
CACAGAATGAAGGTTGAATG+GGG - chr8.4:11058598-11058617 MS.gene064158:intron 40.0%
CCTCTCAAGGATTTCAATCT+GGG - chr8.4:11058637-11058656 MS.gene064158:intron 40.0%
GACTCTAAGCCATTCAATGT+TGG - chr8.4:11058829-11058848 MS.gene064158:CDS 40.0%
GATGTTGAGGTTACAGTTCT+TGG - chr8.4:11059441-11059460 MS.gene064158:intron 40.0%
GTTCCTCTCTCTCCTTTATT+CGG - chr8.4:11057652-11057671 MS.gene064158:CDS 40.0%
GTTTGTAGGGAAAACTACAC+AGG - chr8.4:11058712-11058731 MS.gene064158:CDS 40.0%
TCCTCTCAAGGATTTCAATC+TGG - chr8.4:11058636-11058655 MS.gene064158:intron 40.0%
TTGTTCCCCAAAAATCCCAT+TGG + chr8.4:11057771-11057790 None:intergenic 40.0%
! AGCAATCCAAGCATCAGTTT+TGG - chr8.4:11059127-11059146 MS.gene064158:intron 40.0%
! GAGCAGAAGTCTTTGTTTGT+AGG - chr8.4:11058698-11058717 MS.gene064158:CDS 40.0%
! GAGTCAGTACCTTTTCCTTT+GGG + chr8.4:11059006-11059025 None:intergenic 40.0%
! GATTTCAATCTGGGCTCTAA+AGG - chr8.4:11058646-11058665 MS.gene064158:intron 40.0%
! GTAAAGTACCTTGGAGAACT+AGG + chr8.4:11058424-11058443 None:intergenic 40.0%
!! AAAGGAAAAGGTACTGACTC+GGG - chr8.4:11059006-11059025 MS.gene064158:CDS 40.0%
!! CAAAGGAAAAGGTACTGACT+CGG - chr8.4:11059005-11059024 MS.gene064158:CDS 40.0%
!! CTGTTGGGTTAGCTAAAGTT+AGG + chr8.4:11059050-11059069 None:intergenic 40.0%
!!! CATATATTTTGGAACCTGGG+GGG + chr8.4:11059077-11059096 None:intergenic 40.0%
!!! GCATATATTTTGGAACCTGG+GGG + chr8.4:11059078-11059097 None:intergenic 40.0%
!!! TCCAAGCATCAGTTTTGGAA+AGG - chr8.4:11059132-11059151 MS.gene064158:intron 40.0%
AAGCTCACTTCATCCTGGTT+GGG - chr8.4:11058346-11058365 MS.gene064158:CDS 45.0%
ACGCCCTACTCAAACAAATC+TGG - chr8.4:11057950-11057969 MS.gene064158:intron 45.0%
AGTGCCTCAAGTCATGAACA+TGG - chr8.4:11059342-11059361 MS.gene064158:intron 45.0%
CCCAGATTGAAATCCTTGAG+AGG + chr8.4:11058640-11058659 None:intergenic 45.0%
CTACCGAATAAAGGAGAGAG+AGG + chr8.4:11057658-11057677 None:intergenic 45.0%
GAATCAAGCTCACTTCATCC+TGG - chr8.4:11058341-11058360 MS.gene064158:CDS 45.0%
GAGGAACAAACCGTCATCTT+TGG + chr8.4:11057639-11057658 None:intergenic 45.0%
TCTCCCATACATGCTTGTAG+GGG + chr8.4:11058774-11058793 None:intergenic 45.0%
TGAGGTTACAGTTCTTGGAG+TGG - chr8.4:11059446-11059465 MS.gene064158:intron 45.0%
TGTGCCATGTTCATGACTTG+AGG + chr8.4:11059349-11059368 None:intergenic 45.0%
! ATACCTGGGGTATCTAGTGA+AGG - chr8.4:11059404-11059423 MS.gene064158:intron 45.0%
! ATTTGTTTGAGTAGGGCGTC+TGG + chr8.4:11057949-11057968 None:intergenic 45.0%
! CGAGTCAGTACCTTTTCCTT+TGG + chr8.4:11059007-11059026 None:intergenic 45.0%
! GACAACTACCTAGTTCTCCA+AGG - chr8.4:11058413-11058432 MS.gene064158:CDS 45.0%
!! GGGCTCTAAAGGTTATCTAC+TGG - chr8.4:11058657-11058676 MS.gene064158:intron 45.0%
!! TAGATACCCCAGGTATTGAC+TGG + chr8.4:11059400-11059419 None:intergenic 45.0%
CAAGCTCACTTCATCCTGGT+TGG - chr8.4:11058345-11058364 MS.gene064158:CDS 50.0%
CCATACATGCTTGTAGGGGA+GGG + chr8.4:11058770-11058789 None:intergenic 50.0%
CCTCCCCTACAAGCATGTAT+GGG - chr8.4:11058768-11058787 MS.gene064158:CDS 50.0%
TGTCCTTCACTAGATACCCC+AGG + chr8.4:11059410-11059429 None:intergenic 50.0%
TTGAAGCTGGAGGCCACAAA+TGG - chr8.4:11058138-11058157 MS.gene064158:intron 50.0%
! GAATCGGGGAAGTTTGAAGC+TGG - chr8.4:11058125-11058144 MS.gene064158:intron 50.0%
CCCATACATGCTTGTAGGGG+AGG + chr8.4:11058771-11058790 None:intergenic 55.0%
CCCTCCCCTACAAGCATGTA+TGG - chr8.4:11058767-11058786 MS.gene064158:CDS 55.0%
! AACCTGGGGGGATTGCTGTT+GGG + chr8.4:11059065-11059084 None:intergenic 55.0%
! TCGGGGAAGTTTGAAGCTGG+AGG - chr8.4:11058128-11058147 MS.gene064158:intron 55.0%
AACCCAACAGCAATCCCCCC+AGG - chr8.4:11059060-11059079 MS.gene064158:CDS 60.0%
GAACCTGGGGGGATTGCTGT+TGG + chr8.4:11059066-11059085 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 11057629 11059485 11057629 ID=MS.gene064158
chr8.4 mRNA 11057629 11059485 11057629 ID=MS.gene064158.t1;Parent=MS.gene064158
chr8.4 exon 11059467 11059485 11059467 ID=MS.gene064158.t1.exon1;Parent=MS.gene064158.t1
chr8.4 CDS 11059467 11059485 11059467 ID=cds.MS.gene064158.t1;Parent=MS.gene064158.t1
chr8.4 exon 11058955 11059084 11058955 ID=MS.gene064158.t1.exon2;Parent=MS.gene064158.t1
chr8.4 CDS 11058955 11059084 11058955 ID=cds.MS.gene064158.t1;Parent=MS.gene064158.t1
chr8.4 exon 11058680 11058849 11058680 ID=MS.gene064158.t1.exon3;Parent=MS.gene064158.t1
chr8.4 CDS 11058680 11058849 11058680 ID=cds.MS.gene064158.t1;Parent=MS.gene064158.t1
chr8.4 exon 11058251 11058542 11058251 ID=MS.gene064158.t1.exon4;Parent=MS.gene064158.t1
chr8.4 CDS 11058251 11058542 11058251 ID=cds.MS.gene064158.t1;Parent=MS.gene064158.t1
chr8.4 exon 11057961 11058127 11057961 ID=MS.gene064158.t1.exon5;Parent=MS.gene064158.t1
chr8.4 CDS 11057961 11058127 11057961 ID=cds.MS.gene064158.t1;Parent=MS.gene064158.t1
chr8.4 exon 11057629 11057786 11057629 ID=MS.gene064158.t1.exon6;Parent=MS.gene064158.t1
chr8.4 CDS 11057629 11057786 11057629 ID=cds.MS.gene064158.t1;Parent=MS.gene064158.t1
Gene Sequence

>MS.gene064158

ATGGATACCAAAGATGACGGTTTGTTCCTCTCTCTCCTTTATTCGGTAGCATCTTTAGAAAAGTCACTTTTCATGTGTTTTCATAATATACTTCTTGGCTTGTTTGCGTCTATGCAACATGAAATGTACAATTAATAAACCAATGGGATTTTTGGGGAACAATTTTTTTTTGGTAATGAGTACATAATATATTTCATGGATAATGATGGAATAATAGAATAGTTATATAGATAGCAAGAAAAATAAATATTTAATTTCATAGATAGCAAGAAAAATAAATATTTTATTTCTTAAAAAAAAAAATTAACTCAAGGCAACCAGACGCCCTACTCAAACAAATCTGGAATTTTTCCTATGCTTAGTTATTCTATGTGATTTACTTATAAAAAATAAAATTGCAGGTATTTCTAGATCTATAGTAGAAGCTTCGCCAGTTCATTACATAATGAAAATACAATCATTTTCACTCCTCACTACTAATTCTATAGAGAGATATGAATCGGGGAAGTTTGAAGCTGGAGGCCACAAATGGTATACAATATACATTATATATGTTCCTGCTAAAAATAATTTCATGAATGATAATTTTGGTCTATTTTAGAGTGAATATGTTAAAAATGTGTTTATACCTCACAGGAAGTTAGTTATGTATCCAAGTGGAAACAAAAGCAAGAATGTAAAGGATCATATTTCACTATACTTGGCATTGGAAGAATCAAGCTCACTTCATCCTGGTTGGGAAATCTATGTAAATTTTAAATTGTTTTTATATGATCACAACAATGACAACTACCTAGTTCTCCAAGGTACTTTACCTTTTTTTTTTTTTTTAATATATATTCACTTAATAATTAATTTCCCTCGAAAATAGATTTTGATCTGCTTAAACAGTTTAGTGTTTGACGGTCCCCTAATTTTTTTTTTGGATTGCTGCATTTAATAGATGATGTGAAAAAGGAAAAAAGATTTCACAGAATGAAGGTTGAATGGGGATTTGATCAATTTATTCCTCTCAAGGATTTCAATCTGGGCTCTAAAGGTTATCTACTGGATGATATATGTGCATTTGGAGCAGAAGTCTTTGTTTGTAGGGAAAACTACACAGGTAAAGGAGAATCTTTGATCATGATGAAGGATGCCCTCCCCTACAAGCATGTATGGGAGATTAAAGACTTCTCAAAGTTGGATTCGGAGTGTTGTGACTCTAAGCCATTCAATGTTGGAAACTACAAGTGGTACAAATGCTTAGCCTCCATTTCTTGCTAAACTAAATAACTCCATTCAAAGAGTATTGTTTGAACTCAACAGGAAATTTGACAACTTAGTAATTTACCAAGTTATATGATAACTTTTTGCAGGCAGATAAAACTGTATCCCAAAGGAAAAGGTACTGACTCGGGCAGATATCTTGCTCTGTACCTAACTTTAGCTAACCCAACAGCAATCCCCCCAGGTTCCAAAATATATGCACAGACAATATTACGCATACTTGATCAAAAGCAATCCAAGCATCAGTTTTGGAAAGGTAAAGTTTGTTACACTTTGTTGTGAAATTGGTCAGGCAAAATGCTAGCCATACAAAATGACAGCTGAATGAATAGTTAACGTTGAGAACAACATTTTTTGCATCTCTAAGAAAGCATAAACATAGATCTGATTTGATAATCAAAAGTTCTATTTTTTGTCTGTCTACTTGCAGCTAATTATTGGTTTAGTGCCTCAAGTCATGAACATGGCACATCCAGATTTATTTTGTGCAGTAATTTCACCAGTCAATACCTGGGGTATCTAGTGAAGGACATTTGCTTTGTAGATGTTGAGGTTACAGTTCTTGGAGTGGTTGATGCATTGTCTTGA
Protein sequence

>MS.gene064158.t1

MDTKDDGISRSIVEASPVHYIMKIQSFSLLTTNSIERYESGKFEAGGHKWKLVMYPSGNKSKNVKDHISLYLALEESSSLHPGWEIYVNFKLFLYDHNNDNYLVLQDDVKKEKRFHRMKVEWGFDQFIPLKDFNLGSKGYLLDDICAFGAEVFVCRENYTGKGESLIMMKDALPYKHVWEIKDFSKLDSECCDSKPFNVGNYKWQIKLYPKGKGTDSGRYLALYLTLANPTAIPPGSKIYAQTILRILDQKQSKHQFWKANYWFSASSHEHGTSRFILCSNFTSQYLGYLVKDICFVDVEVTVLGVVDALS