Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064683.t1 | KEH36177.1 | 85.8 | 288 | 30 | 2 | 1 | 288 | 1 | 277 | 4.30E-109 | 404.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064683.t1 | A0A072V2U5 | 85.8 | 288 | 30 | 2 | 1 | 288 | 1 | 277 | 3.1e-109 | 404.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051360 | MS.gene064683 | 0.811725 | 6.06E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064683.t1 | MTR_3g112100 | 87.153 | 288 | 26 | 2 | 1 | 288 | 1 | 277 | 2.33e-172 | 478 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 60 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAAAATTAATTTCCTAATA+TGG | 0.169496 | 3.4:-97098128 | None:intergenic |
| TGAATCTCCTCAACTCTTTC+GGG | 0.202962 | 3.4:-97097938 | None:intergenic |
| TTGAATCTCCTCAACTCTTT+CGG | 0.229356 | 3.4:-97097939 | None:intergenic |
| CTTGGCCCTGACTCCATATT+AGG | 0.296205 | 3.4:+97098115 | MS.gene064683:CDS |
| GACTCTACGCGATCGGTAAC+AGG | 0.314731 | 3.4:-97097672 | None:intergenic |
| TGGTGGAAACGTCGTCGTTT+TGG | 0.336274 | 3.4:-97097488 | None:intergenic |
| GACAACTACTGGAGATAATA+AGG | 0.340624 | 3.4:+97097848 | MS.gene064683:CDS |
| TAAAATAACCGCAGAGGTTC+CGG | 0.353978 | 3.4:+97097584 | MS.gene064683:CDS |
| TTGGTTGGAGAGTTTATATA+TGG | 0.358818 | 3.4:-97097469 | None:intergenic |
| TTTCGGAGGAAAAGTGTTAC+AGG | 0.363849 | 3.4:+97097871 | MS.gene064683:CDS |
| AGATCTTGTTGTTGTTTCTC+AGG | 0.378452 | 3.4:-97097618 | None:intergenic |
| TAATTTCCTAATATGGAGTC+AGG | 0.394555 | 3.4:-97098121 | None:intergenic |
| TACTGGAGATAATAAGGTTT+CGG | 0.401649 | 3.4:+97097854 | MS.gene064683:CDS |
| AGAGTCGCTGAAGAGGGATT+TGG | 0.407242 | 3.4:-97097409 | None:intergenic |
| AAAGCAAGAGAGTGGCAAAA+AGG | 0.415088 | 3.4:-97097326 | None:intergenic |
| GTAACTGCCATGACAGGTTC+AGG | 0.423281 | 3.4:-97097777 | None:intergenic |
| TGCACGACCGTGACAACTAC+TGG | 0.446775 | 3.4:+97097837 | MS.gene064683:CDS |
| TTACCGATCGCGTAGAGTCT+TGG | 0.447245 | 3.4:+97097676 | MS.gene064683:CDS |
| CAACACATGTTTGAAGAAGA+AGG | 0.449031 | 3.4:+97097959 | MS.gene064683:CDS |
| ACAAGTGGAAAATGTATTCA+AGG | 0.458274 | 3.4:-97097299 | None:intergenic |
| TGTTGAAGAATTGAGTAAAG+AGG | 0.462598 | 3.4:+97098004 | MS.gene064683:CDS |
| GTCTCAGAAAACAGAGTACT+TGG | 0.484229 | 3.4:+97098097 | MS.gene064683:CDS |
| GGAGTGTTATGAACGGAGTC+TGG | 0.486988 | 3.4:+97097905 | MS.gene064683:CDS |
| AAGAGCGTAGAGATGAGAAA+AGG | 0.490470 | 3.4:-97097385 | None:intergenic |
| ACATAAGAAGATGATGCAAT+GGG | 0.497256 | 3.4:+97098061 | MS.gene064683:CDS |
| AGATCTTGTAGTGTCTTCGT+TGG | 0.509843 | 3.4:+97097635 | MS.gene064683:CDS |
| TCTTAAAATAACCTCTTCAT+CGG | 0.520835 | 3.4:+97097527 | MS.gene064683:CDS |
| GTGTTACAGGAGAGTTCAAT+CGG | 0.523641 | 3.4:+97097884 | MS.gene064683:CDS |
| AACATAAGAAGATGATGCAA+TGG | 0.524063 | 3.4:+97098060 | MS.gene064683:CDS |
| GTCTCAGTAACTGCCATGAC+AGG | 0.529041 | 3.4:-97097783 | None:intergenic |
| CACCAACAAACACCATGAAA+AGG | 0.532293 | 3.4:+97097256 | None:intergenic |
| CATAGAAAGAGTCGCTGAAG+AGG | 0.548724 | 3.4:-97097416 | None:intergenic |
| TCGCGTAGAGTCTTGGTTGG+AGG | 0.551551 | 3.4:+97097683 | MS.gene064683:CDS |
| AAAACATTGGTAATCGGTGT+CGG | 0.558677 | 3.4:-97097562 | None:intergenic |
| CGTGCAACACGTTGTTGTTG+TGG | 0.561323 | 3.4:-97097820 | None:intergenic |
| TTCTCAGGCAAAGACTCTAC+CGG | 0.563642 | 3.4:-97097603 | None:intergenic |
| AAGACATTGGTTGTAGACAA+TGG | 0.574027 | 3.4:-97097508 | None:intergenic |
| CGATCGCGTAGAGTCTTGGT+TGG | 0.577565 | 3.4:+97097680 | MS.gene064683:CDS |
| ATAGAAAGAGTCGCTGAAGA+GGG | 0.577864 | 3.4:-97097415 | None:intergenic |
| GGAGGAACCTGAACCTGTCA+TGG | 0.581065 | 3.4:+97097770 | MS.gene064683:CDS |
| AGAAAAGGATTTGAGAGCGA+TGG | 0.581973 | 3.4:-97097370 | None:intergenic |
| ATTATCTCCAGTAGTTGTCA+CGG | 0.591123 | 3.4:-97097844 | None:intergenic |
| CGAATAACCCGAAAGAGTTG+AGG | 0.594150 | 3.4:+97097931 | MS.gene064683:CDS |
| AGTAACTAACTCCGATGAAG+AGG | 0.607096 | 3.4:-97097538 | None:intergenic |
| ATATGGAGAGAATGATAACA+TGG | 0.610639 | 3.4:-97097452 | None:intergenic |
| AATTTCCTAATATGGAGTCA+GGG | 0.612963 | 3.4:-97098120 | None:intergenic |
| TGGAGATAATAAGGTTTCGG+AGG | 0.614568 | 3.4:+97097857 | MS.gene064683:CDS |
| CAACCAAGACTCTACGCGAT+CGG | 0.620858 | 3.4:-97097679 | None:intergenic |
| TAAAGAGGAGTTCAAGCGCA+CGG | 0.627740 | 3.4:+97098019 | MS.gene064683:CDS |
| CATGGCAGTTACTGAGACCG+CGG | 0.630430 | 3.4:+97097788 | MS.gene064683:CDS |
| TTCAATCGGAGTGTTATGAA+CGG | 0.647297 | 3.4:+97097898 | MS.gene064683:CDS |
| GTGTTATGAACGGAGTCTGG+TGG | 0.647712 | 3.4:+97097908 | MS.gene064683:CDS |
| TGAAGAAGAAGGAACCGCAG+AGG | 0.656653 | 3.4:+97097970 | MS.gene064683:CDS |
| TAAGAAGATGATGCAATGGG+AGG | 0.667634 | 3.4:+97098064 | MS.gene064683:CDS |
| TGCAATGGGAGGAACAAGCA+CGG | 0.671220 | 3.4:+97098075 | MS.gene064683:CDS |
| AAATGTATTCAAGGTTACGA+CGG | 0.671817 | 3.4:-97097290 | None:intergenic |
| TGTTGTGGTCTCAGTAACCG+CGG | 0.693857 | 3.4:-97097805 | None:intergenic |
| AGACTCTACCGGAACCTCTG+CGG | 0.703722 | 3.4:-97097592 | None:intergenic |
| ACATTGGTTGTAGACAATGG+TGG | 0.711206 | 3.4:-97097505 | None:intergenic |
| ACATAACACAAACTCCTCTG+CGG | 0.740468 | 3.4:-97097984 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTTTAAAACATTGGTAAT+CGG | - | chr3.4:97097571-97097590 | None:intergenic | 15.0% |
| !!! | ACAACAAAATTTTTGCAATT+CGG | + | chr3.4:97097707-97097726 | MS.gene064683:CDS | 20.0% |
| ! | TCTTAAAATAACCTCTTCAT+CGG | + | chr3.4:97097527-97097546 | MS.gene064683:CDS | 25.0% |
| !!! | AAGAGGTTATTTTAAGACAT+TGG | - | chr3.4:97097524-97097543 | None:intergenic | 25.0% |
| AAATGTATTCAAGGTTACGA+CGG | - | chr3.4:97097293-97097312 | None:intergenic | 30.0% | |
| AACATAAGAAGATGATGCAA+TGG | + | chr3.4:97098060-97098079 | MS.gene064683:CDS | 30.0% | |
| ACAAGTGGAAAATGTATTCA+AGG | - | chr3.4:97097302-97097321 | None:intergenic | 30.0% | |
| ACATAAGAAGATGATGCAAT+GGG | + | chr3.4:97098061-97098080 | MS.gene064683:CDS | 30.0% | |
| ATATGGAGAGAATGATAACA+TGG | - | chr3.4:97097455-97097474 | None:intergenic | 30.0% | |
| TACTGGAGATAATAAGGTTT+CGG | + | chr3.4:97097854-97097873 | MS.gene064683:CDS | 30.0% | |
| TGTTGAAGAATTGAGTAAAG+AGG | + | chr3.4:97098004-97098023 | MS.gene064683:CDS | 30.0% | |
| TTGGTTGGAGAGTTTATATA+TGG | - | chr3.4:97097472-97097491 | None:intergenic | 30.0% | |
| ! | AATTTTTGCAATTCGGAAGT+TGG | + | chr3.4:97097714-97097733 | MS.gene064683:CDS | 30.0% |
| !!! | ATGTTTTAAAATAACCGCAG+AGG | + | chr3.4:97097578-97097597 | MS.gene064683:CDS | 30.0% |
| !!! | CTGCGGTTATTTTAAAACAT+TGG | - | chr3.4:97097578-97097597 | None:intergenic | 30.0% |
| ATTATCTCCAGTAGTTGTCA+CGG | - | chr3.4:97097847-97097866 | None:intergenic | 35.0% | |
| CAACACATGTTTGAAGAAGA+AGG | + | chr3.4:97097959-97097978 | MS.gene064683:CDS | 35.0% | |
| GACAACTACTGGAGATAATA+AGG | + | chr3.4:97097848-97097867 | MS.gene064683:CDS | 35.0% | |
| TTGAATCTCCTCAACTCTTT+CGG | - | chr3.4:97097942-97097961 | None:intergenic | 35.0% | |
| ! | AAAACATTGGTAATCGGTGT+CGG | - | chr3.4:97097565-97097584 | None:intergenic | 35.0% |
| ! | AAGACATTGGTTGTAGACAA+TGG | - | chr3.4:97097511-97097530 | None:intergenic | 35.0% |
| ! | AGATTCGTCTCTGATTTTTG+AGG | + | chr3.4:97097737-97097756 | MS.gene064683:CDS | 35.0% |
| ! | TTCAATCGGAGTGTTATGAA+CGG | + | chr3.4:97097898-97097917 | MS.gene064683:CDS | 35.0% |
| !! | AGATCTTGTTGTTGTTTCTC+AGG | - | chr3.4:97097621-97097640 | None:intergenic | 35.0% |
| AAAGCAAGAGAGTGGCAAAA+AGG | - | chr3.4:97097329-97097348 | None:intergenic | 40.0% | |
| ACATAACACAAACTCCTCTG+CGG | - | chr3.4:97097987-97098006 | None:intergenic | 40.0% | |
| AGAAAAGGATTTGAGAGCGA+TGG | - | chr3.4:97097373-97097392 | None:intergenic | 40.0% | |
| AGATCTTGTAGTGTCTTCGT+TGG | + | chr3.4:97097635-97097654 | MS.gene064683:CDS | 40.0% | |
| AGTAACTAACTCCGATGAAG+AGG | - | chr3.4:97097541-97097560 | None:intergenic | 40.0% | |
| ATAGAAAGAGTCGCTGAAGA+GGG | - | chr3.4:97097418-97097437 | None:intergenic | 40.0% | |
| GTGTTACAGGAGAGTTCAAT+CGG | + | chr3.4:97097884-97097903 | MS.gene064683:CDS | 40.0% | |
| TAAAATAACCGCAGAGGTTC+CGG | + | chr3.4:97097584-97097603 | MS.gene064683:CDS | 40.0% | |
| TAAGAAGATGATGCAATGGG+AGG | + | chr3.4:97098064-97098083 | MS.gene064683:CDS | 40.0% | |
| TGAATCTCCTCAACTCTTTC+GGG | - | chr3.4:97097941-97097960 | None:intergenic | 40.0% | |
| TGGAGATAATAAGGTTTCGG+AGG | + | chr3.4:97097857-97097876 | MS.gene064683:CDS | 40.0% | |
| TGGCAAAAAGGTTGAACAAG+TGG | - | chr3.4:97097317-97097336 | None:intergenic | 40.0% | |
| ! | AAGAGCGTAGAGATGAGAAA+AGG | - | chr3.4:97097388-97097407 | None:intergenic | 40.0% |
| ! | ACATTGGTTGTAGACAATGG+TGG | - | chr3.4:97097508-97097527 | None:intergenic | 40.0% |
| ! | GTCTCAGAAAACAGAGTACT+TGG | + | chr3.4:97098097-97098116 | MS.gene064683:CDS | 40.0% |
| !! | GATTTTTGAGGAAACTGTCG+CGG | + | chr3.4:97097749-97097768 | MS.gene064683:CDS | 40.0% |
| !! | TTTCGGAGGAAAAGTGTTAC+AGG | + | chr3.4:97097871-97097890 | MS.gene064683:CDS | 40.0% |
| !!! | TTGCCACTCTCTTGCTTTTT+TGG | + | chr3.4:97097331-97097350 | MS.gene064683:CDS | 40.0% |
| CATAGAAAGAGTCGCTGAAG+AGG | - | chr3.4:97097419-97097438 | None:intergenic | 45.0% | |
| CGAATAACCCGAAAGAGTTG+AGG | + | chr3.4:97097931-97097950 | MS.gene064683:CDS | 45.0% | |
| GTGCCAAAAAAGCAAGAGAG+TGG | - | chr3.4:97097337-97097356 | None:intergenic | 45.0% | |
| TAAAGAGGAGTTCAAGCGCA+CGG | + | chr3.4:97098019-97098038 | MS.gene064683:CDS | 45.0% | |
| TTCTCAGGCAAAGACTCTAC+CGG | - | chr3.4:97097606-97097625 | None:intergenic | 45.0% | |
| AGAGTCGCTGAAGAGGGATT+TGG | - | chr3.4:97097412-97097431 | None:intergenic | 50.0% | |
| CAACCAAGACTCTACGCGAT+CGG | - | chr3.4:97097682-97097701 | None:intergenic | 50.0% | |
| GTAACTGCCATGACAGGTTC+AGG | - | chr3.4:97097780-97097799 | None:intergenic | 50.0% | |
| GTCTCAGTAACTGCCATGAC+AGG | - | chr3.4:97097786-97097805 | None:intergenic | 50.0% | |
| GTGTTATGAACGGAGTCTGG+TGG | + | chr3.4:97097908-97097927 | MS.gene064683:CDS | 50.0% | |
| TGAAGAAGAAGGAACCGCAG+AGG | + | chr3.4:97097970-97097989 | MS.gene064683:CDS | 50.0% | |
| TGCAATGGGAGGAACAAGCA+CGG | + | chr3.4:97098075-97098094 | MS.gene064683:CDS | 50.0% | |
| TGTTGTGGTCTCAGTAACCG+CGG | - | chr3.4:97097808-97097827 | None:intergenic | 50.0% | |
| TTACCGATCGCGTAGAGTCT+TGG | + | chr3.4:97097676-97097695 | MS.gene064683:CDS | 50.0% | |
| ! | CGTGCAACACGTTGTTGTTG+TGG | - | chr3.4:97097823-97097842 | None:intergenic | 50.0% |
| ! | GGAGTGTTATGAACGGAGTC+TGG | + | chr3.4:97097905-97097924 | MS.gene064683:CDS | 50.0% |
| ! | TGGTGGAAACGTCGTCGTTT+TGG | - | chr3.4:97097491-97097510 | None:intergenic | 50.0% |
| !! | GGAAACGTCGTCGTTTTGGT+TGG | - | chr3.4:97097487-97097506 | None:intergenic | 50.0% |
| !! | TTTTGAGGAAACTGTCGCGG+AGG | + | chr3.4:97097752-97097771 | MS.gene064683:CDS | 50.0% |
| AGACTCTACCGGAACCTCTG+CGG | - | chr3.4:97097595-97097614 | None:intergenic | 55.0% | |
| CATGGCAGTTACTGAGACCG+CGG | + | chr3.4:97097788-97097807 | MS.gene064683:CDS | 55.0% | |
| CGATCGCGTAGAGTCTTGGT+TGG | + | chr3.4:97097680-97097699 | MS.gene064683:CDS | 55.0% | |
| GACTCTACGCGATCGGTAAC+AGG | - | chr3.4:97097675-97097694 | None:intergenic | 55.0% | |
| GGAGGAACCTGAACCTGTCA+TGG | + | chr3.4:97097770-97097789 | MS.gene064683:CDS | 55.0% | |
| TCGCGTAGAGTCTTGGTTGG+AGG | + | chr3.4:97097683-97097702 | MS.gene064683:CDS | 55.0% | |
| TGCACGACCGTGACAACTAC+TGG | + | chr3.4:97097837-97097856 | MS.gene064683:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 97097270 | 97098136 | 97097270 | ID=MS.gene064683 |
| chr3.4 | mRNA | 97097270 | 97098136 | 97097270 | ID=MS.gene064683.t1;Parent=MS.gene064683 |
| chr3.4 | exon | 97097270 | 97098136 | 97097270 | ID=MS.gene064683.t1.exon1;Parent=MS.gene064683.t1 |
| chr3.4 | CDS | 97097270 | 97098136 | 97097270 | ID=cds.MS.gene064683.t1;Parent=MS.gene064683.t1 |
| Gene Sequence |
| Protein sequence |
