Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064784.t1 | XP_003603811.3 | 91 | 377 | 32 | 2 | 1 | 376 | 1 | 376 | 1.30E-177 | 632.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064784.t1 | Q6DYE5 | 39.8 | 364 | 198 | 7 | 25 | 373 | 29 | 386 | 1.0e-58 | 228.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064784.t1 | A0A396J381 | 91.0 | 377 | 32 | 2 | 1 | 376 | 1 | 376 | 9.1e-178 | 632.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057208 | MS.gene064784 | 0.803049 | 4.20E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064784.t1 | MTR_3g114460 | 91.351 | 370 | 31 | 1 | 7 | 376 | 9 | 377 | 0.0 | 658 |
| MS.gene064784.t1 | MTR_8g068520 | 51.596 | 376 | 154 | 9 | 13 | 375 | 17 | 377 | 1.48e-117 | 346 |
| MS.gene064784.t1 | MTR_1g025960 | 51.451 | 379 | 152 | 9 | 8 | 375 | 9 | 366 | 3.85e-114 | 337 |
| MS.gene064784.t1 | MTR_1g100200 | 27.596 | 366 | 204 | 9 | 30 | 372 | 10 | 337 | 1.92e-27 | 111 |
| MS.gene064784.t1 | MTR_1g100200 | 27.671 | 365 | 203 | 9 | 31 | 372 | 14 | 340 | 4.03e-27 | 110 |
| MS.gene064784.t1 | MTR_1g100200 | 27.671 | 365 | 203 | 9 | 31 | 372 | 34 | 360 | 6.36e-27 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064784.t1 | AT1G20180 | 40.385 | 364 | 196 | 7 | 25 | 373 | 29 | 386 | 2.85e-69 | 223 |
| MS.gene064784.t1 | AT1G20180 | 37.088 | 364 | 177 | 8 | 25 | 373 | 29 | 355 | 5.61e-58 | 192 |
| MS.gene064784.t1 | AT3G49070 | 29.211 | 380 | 216 | 8 | 12 | 374 | 16 | 359 | 3.98e-33 | 127 |
| MS.gene064784.t1 | AT3G49070 | 29.211 | 380 | 216 | 8 | 12 | 374 | 56 | 399 | 6.58e-33 | 127 |
| MS.gene064784.t1 | AT3G19250 | 22.632 | 380 | 261 | 10 | 1 | 372 | 1 | 355 | 6.07e-12 | 66.6 |
| MS.gene064784.t1 | AT3G19330 | 26.216 | 370 | 237 | 13 | 14 | 372 | 28 | 372 | 1.29e-11 | 65.9 |
| MS.gene064784.t1 | AT3G19330 | 26.216 | 370 | 237 | 13 | 14 | 372 | 28 | 372 | 1.29e-11 | 65.9 |
Find 56 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATTGCCAAACTAACTTTA+TGG | 0.110914 | 3.4:+98130462 | MS.gene064784:CDS |
| TTTGCTTTCCATAGCATAAT+TGG | 0.142059 | 3.4:+98130148 | MS.gene064784:CDS |
| GCGGAAGTTAATAACATTAT+TGG | 0.198214 | 3.4:-98129972 | None:intergenic |
| CGTCAAACTAGATTTGATTA+TGG | 0.245353 | 3.4:+98129809 | MS.gene064784:CDS |
| AAGAGATCAAAGTGGGAAAA+TGG | 0.266547 | 3.4:+98130223 | MS.gene064784:CDS |
| TTATATAGTGATAAATGATT+TGG | 0.292938 | 3.4:+98130303 | MS.gene064784:CDS |
| GATATTTGTGTGAAGAATTA+TGG | 0.298600 | 3.4:+98130388 | MS.gene064784:CDS |
| TGTAACAAAGCTCAAGGATA+TGG | 0.306198 | 3.4:+98129596 | MS.gene064784:CDS |
| TGTCATATTCAACGACTCTT+TGG | 0.315226 | 3.4:-98129862 | None:intergenic |
| ACTGATCCATAAAGTTAGTT+TGG | 0.327126 | 3.4:-98130468 | None:intergenic |
| TATGTAGTTGGTGGATCCTT+TGG | 0.338019 | 3.4:+98130187 | MS.gene064784:CDS |
| AGAAGAAGAAGAAGTGGAAC+AGG | 0.349044 | 3.4:-98129657 | None:intergenic |
| CGTTTCAGAAACCGCTCTTT+TGG | 0.357402 | 3.4:+98130111 | MS.gene064784:CDS |
| TGCAAGAAAGTTGGAGGAAT+CGG | 0.391183 | 3.4:+98130082 | MS.gene064784:CDS |
| GGTTAAGAGGTTAGAGGATG+AGG | 0.399784 | 3.4:+98130342 | MS.gene064784:CDS |
| GCTGCTGCCCCTTATGTAGT+TGG | 0.407925 | 3.4:+98130175 | MS.gene064784:CDS |
| TTCTTTCTTCCGAAGAAGAT+CGG | 0.414371 | 3.4:+98129249 | MS.gene064784:CDS |
| TTTGTAACAATTTCTTGTCT+AGG | 0.414371 | 3.4:-98129689 | None:intergenic |
| TTGCTTTCCATAGCATAATT+GGG | 0.423917 | 3.4:+98130149 | MS.gene064784:CDS |
| AAGAGGTTATGCAAGAAAGT+TGG | 0.425159 | 3.4:+98130073 | MS.gene064784:CDS |
| AAGGATCCACCAACTACATA+AGG | 0.429019 | 3.4:-98130184 | None:intergenic |
| TGCTGCGAGAAAATCCTTCA+AGG | 0.432150 | 3.4:+98129782 | MS.gene064784:CDS |
| CAAGTTGATGTTGCTGCAAA+AGG | 0.432286 | 3.4:+98130277 | MS.gene064784:CDS |
| AAGAATTATGGTCATGGTAA+TGG | 0.433359 | 3.4:+98130400 | MS.gene064784:CDS |
| TTAGCTCACCCGATCTTCTT+CGG | 0.435671 | 3.4:-98129258 | None:intergenic |
| TAGTTTGACGAACACCTTGA+AGG | 0.455617 | 3.4:-98129796 | None:intergenic |
| TCTTTCTTCCGAAGAAGATC+GGG | 0.456820 | 3.4:+98129250 | MS.gene064784:CDS |
| CCGTATGGTTAAGAGGTTAG+AGG | 0.460969 | 3.4:+98130336 | MS.gene064784:CDS |
| AACTAACTTTATGGATCAGT+TGG | 0.461239 | 3.4:+98130471 | MS.gene064784:CDS |
| CTATGAGCCGTATGGTTAAG+AGG | 0.474901 | 3.4:+98130329 | MS.gene064784:CDS |
| AAGTTGATGTTGCTGCAAAA+GGG | 0.480653 | 3.4:+98130278 | MS.gene064784:CDS |
| AGGATCCACCAACTACATAA+GGG | 0.488584 | 3.4:-98130183 | None:intergenic |
| GCAAAGACATGCTTCTAAGC+TGG | 0.506421 | 3.4:-98129762 | None:intergenic |
| TAGAGGATGAGGTTGAACAT+TGG | 0.517451 | 3.4:+98130353 | MS.gene064784:CDS |
| GAAGAAGAAGAGATCAAAGT+GGG | 0.525471 | 3.4:+98130216 | MS.gene064784:CDS |
| TGAGGTTGAACATTGGAGAG+AGG | 0.530933 | 3.4:+98130360 | MS.gene064784:CDS |
| AGGTTATGCAAGAAAGTTGG+AGG | 0.533933 | 3.4:+98130076 | MS.gene064784:CDS |
| CTTCTTCTTCATCAAACCAA+AGG | 0.546523 | 3.4:-98130203 | None:intergenic |
| CAGCTAACCCAATTATGCTA+TGG | 0.556147 | 3.4:-98130156 | None:intergenic |
| GGATCAGTTGGAAGAGCTTG+AGG | 0.556162 | 3.4:+98130483 | MS.gene064784:CDS |
| GCTGCCCCTTATGTAGTTGG+TGG | 0.559630 | 3.4:+98130178 | MS.gene064784:CDS |
| AGTTGATGTTGCTGCAAAAG+GGG | 0.563403 | 3.4:+98130279 | MS.gene064784:CDS |
| TAAGGTGCATCATCTTCTAG+TGG | 0.565366 | 3.4:+98129727 | MS.gene064784:CDS |
| GACATATGTAACAAAGCTCA+AGG | 0.571855 | 3.4:+98129590 | MS.gene064784:CDS |
| CCTCTAACCTCTTAACCATA+CGG | 0.573081 | 3.4:-98130336 | None:intergenic |
| AATTTGTTCTGAATGCATTG+AGG | 0.584357 | 3.4:-98129559 | None:intergenic |
| TGAAGAAGAAGAGATCAAAG+TGG | 0.592386 | 3.4:+98130215 | MS.gene064784:CDS |
| TTTGGATACTATGAGCCGTA+TGG | 0.601069 | 3.4:+98130321 | MS.gene064784:CDS |
| ATTATGGTCATGGTAATGGA+AGG | 0.610111 | 3.4:+98130404 | MS.gene064784:CDS |
| AATATTGAAGATGGTTCTGA+GGG | 0.614569 | 3.4:+98130432 | MS.gene064784:CDS |
| AAGGTGTGTAATATTGAAGA+TGG | 0.621343 | 3.4:+98130423 | MS.gene064784:CDS |
| ATTATCGAAAGAACGTGATG+TGG | 0.633657 | 3.4:+98129496 | MS.gene064784:CDS |
| GGATCCACCAACTACATAAG+GGG | 0.639730 | 3.4:-98130182 | None:intergenic |
| TAATATTGAAGATGGTTCTG+AGG | 0.641414 | 3.4:+98130431 | MS.gene064784:CDS |
| TGTGTGAAGAATTATGGTCA+TGG | 0.650409 | 3.4:+98130394 | MS.gene064784:CDS |
| TAACAAAGCCACCAAAAGAG+CGG | 0.690854 | 3.4:-98130122 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTAAATTAAAAATAAA+CGG | - | chr3.4:98129315-98129334 | None:intergenic | 0.0% |
| !! | TAACTAATTAATTAATTACT+TGG | - | chr3.4:98129408-98129427 | None:intergenic | 10.0% |
| !! | TTATATAGTGATAAATGATT+TGG | + | chr3.4:98130303-98130322 | MS.gene064784:CDS | 15.0% |
| !! | GAAATAATTAAAAAACATGC+AGG | + | chr3.4:98129467-98129486 | MS.gene064784:intron | 20.0% |
| !! | GAAGAAAAATAACAATAAAG+AGG | + | chr3.4:98130056-98130075 | MS.gene064784:CDS | 20.0% |
| ! | AAAAATGATCAAGTGCTTTA+AGG | + | chr3.4:98129709-98129728 | MS.gene064784:CDS | 25.0% |
| ! | ATAATTAAAAAACATGCAGG+TGG | + | chr3.4:98129470-98129489 | MS.gene064784:intron | 25.0% |
| ! | GATATTTGTGTGAAGAATTA+TGG | + | chr3.4:98130388-98130407 | MS.gene064784:CDS | 25.0% |
| ! | TTTGTAACAATTTCTTGTCT+AGG | - | chr3.4:98129692-98129711 | None:intergenic | 25.0% |
| AACTAACTTTATGGATCAGT+TGG | + | chr3.4:98130471-98130490 | MS.gene064784:CDS | 30.0% | |
| AAGAATTATGGTCATGGTAA+TGG | + | chr3.4:98130400-98130419 | MS.gene064784:CDS | 30.0% | |
| AAGGTGTGTAATATTGAAGA+TGG | + | chr3.4:98130423-98130442 | MS.gene064784:CDS | 30.0% | |
| ACTGATCCATAAAGTTAGTT+TGG | - | chr3.4:98130471-98130490 | None:intergenic | 30.0% | |
| CGTCAAACTAGATTTGATTA+TGG | + | chr3.4:98129809-98129828 | MS.gene064784:CDS | 30.0% | |
| GCGGAAGTTAATAACATTAT+TGG | - | chr3.4:98129975-98129994 | None:intergenic | 30.0% | |
| TGATTGCCAAACTAACTTTA+TGG | + | chr3.4:98130462-98130481 | MS.gene064784:CDS | 30.0% | |
| TTGCTTTCCATAGCATAATT+GGG | + | chr3.4:98130149-98130168 | MS.gene064784:CDS | 30.0% | |
| TTTGCTTTCCATAGCATAAT+TGG | + | chr3.4:98130148-98130167 | MS.gene064784:CDS | 30.0% | |
| ! | AATTTGTTCTGAATGCATTG+AGG | - | chr3.4:98129562-98129581 | None:intergenic | 30.0% |
| ! | TAATATTGAAGATGGTTCTG+AGG | + | chr3.4:98130431-98130450 | MS.gene064784:CDS | 30.0% |
| ! | TGTGTTTTTCTTGAATATCG+CGG | - | chr3.4:98129994-98130013 | None:intergenic | 30.0% |
| !! | AATATTGAAGATGGTTCTGA+GGG | + | chr3.4:98130432-98130451 | MS.gene064784:CDS | 30.0% |
| AAGAGATCAAAGTGGGAAAA+TGG | + | chr3.4:98130223-98130242 | MS.gene064784:CDS | 35.0% | |
| AAGAGGTTATGCAAGAAAGT+TGG | + | chr3.4:98130073-98130092 | MS.gene064784:CDS | 35.0% | |
| ATTAAAAAACATGCAGGTGG+TGG | + | chr3.4:98129473-98129492 | MS.gene064784:intron | 35.0% | |
| ATTATCGAAAGAACGTGATG+TGG | + | chr3.4:98129496-98129515 | MS.gene064784:CDS | 35.0% | |
| CTTCTTCTTCATCAAACCAA+AGG | - | chr3.4:98130206-98130225 | None:intergenic | 35.0% | |
| GAAGAAGAAGAGATCAAAGT+GGG | + | chr3.4:98130216-98130235 | MS.gene064784:CDS | 35.0% | |
| GAATTGAACTCCAATATCAG+AGG | + | chr3.4:98129368-98129387 | MS.gene064784:intron | 35.0% | |
| GACATATGTAACAAAGCTCA+AGG | + | chr3.4:98129590-98129609 | MS.gene064784:CDS | 35.0% | |
| TAGCAAAGAAGAAGAAGAAG+TGG | - | chr3.4:98129666-98129685 | None:intergenic | 35.0% | |
| TGAAGAAGAAGAGATCAAAG+TGG | + | chr3.4:98130215-98130234 | MS.gene064784:CDS | 35.0% | |
| TGTAACAAAGCTCAAGGATA+TGG | + | chr3.4:98129596-98129615 | MS.gene064784:CDS | 35.0% | |
| TGTCATATTCAACGACTCTT+TGG | - | chr3.4:98129865-98129884 | None:intergenic | 35.0% | |
| TGTGTGAAGAATTATGGTCA+TGG | + | chr3.4:98130394-98130413 | MS.gene064784:CDS | 35.0% | |
| TTCTTTCTTCCGAAGAAGAT+CGG | + | chr3.4:98129249-98129268 | MS.gene064784:CDS | 35.0% | |
| !! | AAGTTGATGTTGCTGCAAAA+GGG | + | chr3.4:98130278-98130297 | MS.gene064784:CDS | 35.0% |
| !! | ATTATGGTCATGGTAATGGA+AGG | + | chr3.4:98130404-98130423 | MS.gene064784:CDS | 35.0% |
| AAGGATCCACCAACTACATA+AGG | - | chr3.4:98130187-98130206 | None:intergenic | 40.0% | |
| AGAAGAAGAAGAAGTGGAAC+AGG | - | chr3.4:98129660-98129679 | None:intergenic | 40.0% | |
| AGGATCCACCAACTACATAA+GGG | - | chr3.4:98130186-98130205 | None:intergenic | 40.0% | |
| AGGTTATGCAAGAAAGTTGG+AGG | + | chr3.4:98130076-98130095 | MS.gene064784:CDS | 40.0% | |
| CAGCTAACCCAATTATGCTA+TGG | - | chr3.4:98130159-98130178 | None:intergenic | 40.0% | |
| CCTCTAACCTCTTAACCATA+CGG | - | chr3.4:98130339-98130358 | None:intergenic | 40.0% | |
| TAACAAAGCCACCAAAAGAG+CGG | - | chr3.4:98130125-98130144 | None:intergenic | 40.0% | |
| TAAGGTGCATCATCTTCTAG+TGG | + | chr3.4:98129727-98129746 | MS.gene064784:CDS | 40.0% | |
| TAGAGGATGAGGTTGAACAT+TGG | + | chr3.4:98130353-98130372 | MS.gene064784:CDS | 40.0% | |
| TAGTTTGACGAACACCTTGA+AGG | - | chr3.4:98129799-98129818 | None:intergenic | 40.0% | |
| TCTTTCTTCCGAAGAAGATC+GGG | + | chr3.4:98129250-98129269 | MS.gene064784:CDS | 40.0% | |
| TGCAAGAAAGTTGGAGGAAT+CGG | + | chr3.4:98130082-98130101 | MS.gene064784:CDS | 40.0% | |
| TTGAACTCCAATATCAGAGG+AGG | + | chr3.4:98129371-98129390 | MS.gene064784:intron | 40.0% | |
| TTTGGATACTATGAGCCGTA+TGG | + | chr3.4:98130321-98130340 | MS.gene064784:CDS | 40.0% | |
| ! | AGTTGATGTTGCTGCAAAAG+GGG | + | chr3.4:98130279-98130298 | MS.gene064784:CDS | 40.0% |
| !! | CAAGTTGATGTTGCTGCAAA+AGG | + | chr3.4:98130277-98130296 | MS.gene064784:CDS | 40.0% |
| !! | TATGTAGTTGGTGGATCCTT+TGG | + | chr3.4:98130187-98130206 | MS.gene064784:CDS | 40.0% |
| AAGCACACCTCCTCTGATAT+TGG | - | chr3.4:98129381-98129400 | None:intergenic | 45.0% | |
| CCGTATGGTTAAGAGGTTAG+AGG | + | chr3.4:98130336-98130355 | MS.gene064784:CDS | 45.0% | |
| CTATGAGCCGTATGGTTAAG+AGG | + | chr3.4:98130329-98130348 | MS.gene064784:CDS | 45.0% | |
| GCAAAGACATGCTTCTAAGC+TGG | - | chr3.4:98129765-98129784 | None:intergenic | 45.0% | |
| GGATCCACCAACTACATAAG+GGG | - | chr3.4:98130185-98130204 | None:intergenic | 45.0% | |
| GGTTAAGAGGTTAGAGGATG+AGG | + | chr3.4:98130342-98130361 | MS.gene064784:CDS | 45.0% | |
| TGAGGTTGAACATTGGAGAG+AGG | + | chr3.4:98130360-98130379 | MS.gene064784:CDS | 45.0% | |
| TGCTGCGAGAAAATCCTTCA+AGG | + | chr3.4:98129782-98129801 | MS.gene064784:CDS | 45.0% | |
| TTAGCTCACCCGATCTTCTT+CGG | - | chr3.4:98129261-98129280 | None:intergenic | 45.0% | |
| ! | AATATCAGAGGAGGTGTGCT+TGG | + | chr3.4:98129380-98129399 | MS.gene064784:intron | 45.0% |
| ! | CGTTTCAGAAACCGCTCTTT+TGG | + | chr3.4:98130111-98130130 | MS.gene064784:CDS | 45.0% |
| ! | TTCAGAAACCGCTCTTTTGG+TGG | + | chr3.4:98130114-98130133 | MS.gene064784:CDS | 45.0% |
| GGATCAGTTGGAAGAGCTTG+AGG | + | chr3.4:98130483-98130502 | MS.gene064784:CDS | 50.0% | |
| GCTGCCCCTTATGTAGTTGG+TGG | + | chr3.4:98130178-98130197 | MS.gene064784:CDS | 55.0% | |
| GCTGCTGCCCCTTATGTAGT+TGG | + | chr3.4:98130175-98130194 | MS.gene064784:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 98129235 | 98130585 | 98129235 | ID=MS.gene064784 |
| chr3.4 | mRNA | 98129235 | 98130585 | 98129235 | ID=MS.gene064784.t1;Parent=MS.gene064784 |
| chr3.4 | exon | 98129235 | 98129271 | 98129235 | ID=MS.gene064784.t1.exon1;Parent=MS.gene064784.t1 |
| chr3.4 | CDS | 98129235 | 98129271 | 98129235 | ID=cds.MS.gene064784.t1;Parent=MS.gene064784.t1 |
| chr3.4 | exon | 98129489 | 98130585 | 98129489 | ID=MS.gene064784.t1.exon2;Parent=MS.gene064784.t1 |
| chr3.4 | CDS | 98129489 | 98130585 | 98129489 | ID=cds.MS.gene064784.t1;Parent=MS.gene064784.t1 |
| Gene Sequence |
| Protein sequence |
