Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064815.t1 | XP_013462246.1 | 98.1 | 107 | 2 | 0 | 1 | 107 | 1 | 107 | 2.30E-47 | 198 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064815.t1 | A0A072V3Q4 | 98.1 | 107 | 2 | 0 | 1 | 107 | 1 | 107 | 1.6e-47 | 198.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05595 | MS.gene064815 | -0.821946 | 3.08E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064815.t1 | MTR_3g115060 | 98.131 | 107 | 2 | 0 | 1 | 107 | 1 | 107 | 4.64e-73 | 212 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064815.t1 | AT1G20430 | 65.882 | 85 | 29 | 0 | 23 | 107 | 29 | 113 | 1.05e-33 | 113 |
Find 21 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCTTAAGCATGAAATCTT+TGG | 0.222509 | 3.4:-98470552 | MS.gene064815:CDS |
| CTCTTAAGCATGAAATCTTT+GGG | 0.311239 | 3.4:-98470551 | MS.gene064815:CDS |
| CCGAATGCCGCCGGTGCCGT+TGG | 0.453325 | 3.4:-98470659 | MS.gene064815:CDS |
| CCTCTTGGAGTCCATGGATT+CGG | 0.472053 | 3.4:+98470519 | None:intergenic |
| CCAACGGCACCGGCGGCATT+CGG | 0.480360 | 3.4:+98470659 | None:intergenic |
| TCAGATCATGAGTATCCTCT+TGG | 0.496481 | 3.4:+98470504 | None:intergenic |
| GAGTATCCTCTTGGAGTCCA+TGG | 0.510166 | 3.4:+98470513 | None:intergenic |
| ATTCGGTCGCACTGAGGAGG+AGG | 0.524049 | 3.4:+98470676 | None:intergenic |
| GAAGCTTCTCAAGATTCTAC+TGG | 0.533479 | 3.4:-98470422 | MS.gene064815:CDS |
| GGACAAAAGTACCGAATCCA+TGG | 0.544794 | 3.4:-98470530 | MS.gene064815:CDS |
| CTTCTTCGAACCAACGGCAC+CGG | 0.551741 | 3.4:+98470649 | None:intergenic |
| TAGCTTCTTCTTCGAACCAA+CGG | 0.568765 | 3.4:+98470643 | None:intergenic |
| CTTCGAACCAACGGCACCGG+CGG | 0.570741 | 3.4:+98470652 | None:intergenic |
| GGCATTCGGTCGCACTGAGG+AGG | 0.571642 | 3.4:+98470673 | None:intergenic |
| CACTGAGGAGGAGGAAGACA+TGG | 0.572521 | 3.4:+98470685 | None:intergenic |
| TCAGTGCGACCGAATGCCGC+CGG | 0.579043 | 3.4:-98470668 | MS.gene064815:CDS |
| AAGAGGATACTCATGATCTG+AGG | 0.589972 | 3.4:-98470502 | MS.gene064815:CDS |
| AGAGGATACTCATGATCTGA+GGG | 0.604407 | 3.4:-98470501 | MS.gene064815:CDS |
| CCGAATCCATGGACTCCAAG+AGG | 0.665176 | 3.4:-98470519 | MS.gene064815:CDS |
| GGCGGCATTCGGTCGCACTG+AGG | 0.704327 | 3.4:+98470670 | None:intergenic |
| ATTCATTGAAGAGACTACGA+AGG | 0.718138 | 3.4:+98470384 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTCTTAAGCATGAAATCTTT+GGG | - | chr3.4:98470514-98470533 | MS.gene064815:CDS | 30.0% | |
| TCTCTTAAGCATGAAATCTT+TGG | - | chr3.4:98470513-98470532 | MS.gene064815:CDS | 30.0% | |
| ! | CTTGATGTTTTTCATACGAT+CGG | + | chr3.4:98470608-98470627 | None:intergenic | 30.0% |
| ATTCATTGAAGAGACTACGA+AGG | + | chr3.4:98470684-98470703 | None:intergenic | 35.0% | |
| ! | ATTCAACTTTTCGCAATGAC+TGG | - | chr3.4:98470484-98470503 | MS.gene064815:CDS | 35.0% |
| AAGAGGATACTCATGATCTG+AGG | - | chr3.4:98470563-98470582 | MS.gene064815:CDS | 40.0% | |
| AGAGGATACTCATGATCTGA+GGG | - | chr3.4:98470564-98470583 | MS.gene064815:CDS | 40.0% | |
| GAAGCTTCTCAAGATTCTAC+TGG | - | chr3.4:98470643-98470662 | MS.gene064815:CDS | 40.0% | |
| TAGCTTCTTCTTCGAACCAA+CGG | + | chr3.4:98470425-98470444 | None:intergenic | 40.0% | |
| TCAGATCATGAGTATCCTCT+TGG | + | chr3.4:98470564-98470583 | None:intergenic | 40.0% | |
| !! | GGACAAAAGTACCGAATCCA+TGG | - | chr3.4:98470535-98470554 | MS.gene064815:CDS | 45.0% |
| CCTCTTGGAGTCCATGGATT+CGG | + | chr3.4:98470549-98470568 | None:intergenic | 50.0% | |
| GAGTATCCTCTTGGAGTCCA+TGG | + | chr3.4:98470555-98470574 | None:intergenic | 50.0% | |
| CCGAATCCATGGACTCCAAG+AGG | - | chr3.4:98470546-98470565 | MS.gene064815:CDS | 55.0% | |
| CTTCTTCGAACCAACGGCAC+CGG | + | chr3.4:98470419-98470438 | None:intergenic | 55.0% | |
| ATTCGGTCGCACTGAGGAGG+AGG | + | chr3.4:98470392-98470411 | None:intergenic | 60.0% | |
| GGCATTCGGTCGCACTGAGG+AGG | + | chr3.4:98470395-98470414 | None:intergenic | 65.0% | |
| TCAGTGCGACCGAATGCCGC+CGG | - | chr3.4:98470397-98470416 | MS.gene064815:CDS | 65.0% | |
| ! | CTTCGAACCAACGGCACCGG+CGG | + | chr3.4:98470416-98470435 | None:intergenic | 65.0% |
| GGCGGCATTCGGTCGCACTG+AGG | + | chr3.4:98470398-98470417 | None:intergenic | 70.0% | |
| !! | CCAACGGCACCGGCGGCATT+CGG | + | chr3.4:98470409-98470428 | None:intergenic | 70.0% |
| !! | CCGAATGCCGCCGGTGCCGT+TGG | - | chr3.4:98470406-98470425 | MS.gene064815:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 98470382 | 98470705 | 98470382 | ID=MS.gene064815 |
| chr3.4 | mRNA | 98470382 | 98470705 | 98470382 | ID=MS.gene064815.t1;Parent=MS.gene064815 |
| chr3.4 | exon | 98470382 | 98470705 | 98470382 | ID=MS.gene064815.t1.exon1;Parent=MS.gene064815.t1 |
| chr3.4 | CDS | 98470382 | 98470705 | 98470382 | ID=cds.MS.gene064815.t1;Parent=MS.gene064815.t1 |
| Gene Sequence |
| Protein sequence |
