AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene064950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064950.t1 MTR_1g046210 96.536 433 15 0 1 433 1 433 0.0 881
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064950.t1 AT2G05320 65.349 430 144 2 4 432 5 430 0.0 602

Find 116 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGCATCTTTACCAATATT+TGG 0.160032 1.4:+30687376 None:intergenic
GTTTCTTTGATGATTATAAT+TGG 0.161902 1.4:-30686721 MS.gene064950:CDS
TTTGTTGCCACCAAGGAATT+TGG 0.239817 1.4:-30687407 MS.gene064950:CDS
TTGGATTTGTATCCAAATAT+TGG 0.255178 1.4:-30687388 MS.gene064950:CDS
GGTTATAAAGCCGGGTTTAA+AGG 0.263690 1.4:-30686470 MS.gene064950:CDS
GTTTAAAGGTTGGGGAGGTT+GGG 0.274547 1.4:-30686456 MS.gene064950:CDS
GGTTTAAAGGTTGGGGAGGT+TGG 0.289854 1.4:-30686457 MS.gene064950:CDS
TTTAAAGGTTGGGGAGGTTG+GGG 0.302739 1.4:-30686455 MS.gene064950:CDS
ATAAGATCATTGGTGGTATT+AGG 0.307860 1.4:-30687225 MS.gene064950:CDS
GAAACCACGTCTTAGAGATT+TGG 0.321204 1.4:-30687641 MS.gene064950:CDS
CTTCAAATCGCGACGATAAC+CGG 0.329379 1.4:+30687492 None:intergenic
TGGGAAATGTGGTTTGCATC+AGG 0.335039 1.4:-30686612 MS.gene064950:CDS
TTGGAAGAAAATACACAATA+AGG 0.346190 1.4:-30686756 MS.gene064950:CDS
ATGTGGTTTGCATCAGGGTC+AGG 0.352273 1.4:-30686606 MS.gene064950:CDS
CAAGGGAGATACAGTGCCTT+TGG 0.365705 1.4:+30686379 None:intergenic
TAAAGCCGGGTTTAAAGGTT+GGG 0.368431 1.4:-30686465 MS.gene064950:CDS
GGACTAGTGCTGTTCACTTT+GGG 0.377896 1.4:-30686631 MS.gene064950:CDS
TTTCTTTGATGATTATAATT+GGG 0.381332 1.4:-30686720 MS.gene064950:CDS
TTAAAGGTTGGGGAGGTTGG+GGG 0.381860 1.4:-30686454 MS.gene064950:CDS
GAAAGGGTTGATGATGGTTC+CGG 0.383637 1.4:-30687511 MS.gene064950:CDS
AGTGAGACGAGAGCGCATTC+TGG 0.383801 1.4:-30686986 MS.gene064950:CDS
GAGAGTCTTGCTAAGGTTGT+AGG 0.386703 1.4:-30687301 MS.gene064950:CDS
TAAAGGTTGGGGAGGTTGGG+GGG 0.398412 1.4:-30686453 MS.gene064950:CDS
TTGAAACTAATGATTTAGAA+AGG 0.399269 1.4:-30687528 MS.gene064950:CDS
GAAGGGAATCCTGATCAGTA+TGG 0.399926 1.4:-30687085 MS.gene064950:CDS
GGAAACAAATGAGGCGAATA+CGG 0.413406 1.4:+30687175 None:intergenic
GCGACTGTTTATCCCTCATT+TGG 0.423496 1.4:-30686683 MS.gene064950:CDS
TAGTCACGATTGTTACTTTG+AGG 0.425100 1.4:-30687254 MS.gene064950:CDS
GTGGTGTTTCTTACTTTATG+TGG 0.425344 1.4:-30687598 MS.gene064950:CDS
TAGTTTAGTTGCGGAGAGAA+TGG 0.426139 1.4:-30686810 MS.gene064950:CDS
AGTTTAGTTGCGGAGAGAAT+GGG 0.429923 1.4:-30686809 MS.gene064950:CDS
AGGACTAGTGCTGTTCACTT+TGG 0.433822 1.4:-30686632 MS.gene064950:CDS
GGATACAAATCCAAATTCCT+TGG 0.434662 1.4:+30687397 None:intergenic
TACGAACATTGATTCAGATT+GGG 0.435160 1.4:-30686519 MS.gene064950:CDS
GGTTTCGATTGGTGATTGTA+AGG 0.444803 1.4:-30687137 MS.gene064950:CDS
TGTAAATTCCGATACGCATT+GGG 0.445185 1.4:+30686926 None:intergenic
GAAACCCCTGGAAAACTATT+CGG 0.447339 1.4:+30687154 None:intergenic
CTGTAAATTCCGATACGCAT+TGG 0.453724 1.4:+30686925 None:intergenic
GTGAGACGAGAGCGCATTCT+GGG 0.454046 1.4:-30686985 MS.gene064950:CDS
GAGGAAATGAATAAGATCAT+TGG 0.454084 1.4:-30687235 MS.gene064950:CDS
TTACGAACATTGATTCAGAT+TGG 0.458017 1.4:-30686520 MS.gene064950:CDS
CCAGAAACTGAACTCTTACA+AGG 0.460434 1.4:+30686361 None:intergenic
TGCTTCAACGACACAATCTT+CGG 0.463204 1.4:+30687049 None:intergenic
TTCTAACTTCGTTGAAGCCT+AGG 0.464553 1.4:-30686901 MS.gene064950:CDS
TAACCGGCCGCAGTATCTTA+AGG 0.468443 1.4:-30687332 MS.gene064950:CDS
ATCAGCCTGGTTATAAAGCC+GGG 0.475227 1.4:-30686478 MS.gene064950:CDS
CGGTGGTTCCCATACTGATC+AGG 0.478161 1.4:+30687076 None:intergenic
AGTGAACAGCACTAGTCCTT+GGG 0.478854 1.4:+30686636 None:intergenic
TGAAACTAATGATTTAGAAA+GGG 0.487518 1.4:-30687527 MS.gene064950:CDS
TAACAATCAGGACATTTCCT+AGG 0.500121 1.4:+30686884 None:intergenic
AAGTGAACAGCACTAGTCCT+TGG 0.502432 1.4:+30686635 None:intergenic
ACTCGTTTAATCGAACGGTT+TGG 0.504792 1.4:-30686775 MS.gene064950:CDS
AAATTTGCAGCATAACAATC+AGG 0.508654 1.4:+30686872 None:intergenic
AATCAGCCTGGTTATAAAGC+CGG 0.509207 1.4:-30686479 MS.gene064950:CDS
GGGAAATGTGGTTTGCATCA+GGG 0.510464 1.4:-30686611 MS.gene064950:CDS
GATTTAGAAAGGGTTGATGA+TGG 0.515891 1.4:-30687517 MS.gene064950:CDS
CGGCAAAGCTCAAGCATAAG+TGG 0.517983 1.4:+30686417 None:intergenic
GGTGGATGATGAACACGGTA+TGG 0.518093 1.4:-30687018 MS.gene064950:CDS
TGTGGTTTGCATCAGGGTCA+GGG 0.518341 1.4:-30686605 MS.gene064950:CDS
TTGTGTCGTTGAAGCATCAT+TGG 0.520834 1.4:-30687042 MS.gene064950:CDS
ATAAAGCCGGGTTTAAAGGT+TGG 0.530281 1.4:-30686466 MS.gene064950:CDS
AAAGCCGGGTTTAAAGGTTG+GGG 0.534299 1.4:-30686464 MS.gene064950:CDS
AGAGTCTTGCTAAGGTTGTA+GGG 0.539393 1.4:-30687300 MS.gene064950:CDS
GCTGTTCACTTTGGGAAATG+TGG 0.541333 1.4:-30686623 MS.gene064950:CDS
ACAAATCACTCTGCTGTGGA+AGG 0.550303 1.4:+30687452 None:intergenic
CGTTGAAGCATCATTGGTGG+TGG 0.550314 1.4:-30687036 MS.gene064950:CDS
CAATCACCAATCGAAACCCC+TGG 0.550689 1.4:+30687142 None:intergenic
TCGTGTAGAAGATCATGATA+AGG 0.553739 1.4:-30686543 MS.gene064950:CDS
GGGAGATACAGTGCCTTTGG+AGG 0.556951 1.4:+30686382 None:intergenic
AGTTGAATTTGTTGCCACCA+AGG 0.557616 1.4:-30687414 MS.gene064950:CDS
GTGGTTTGCATCAGGGTCAG+GGG 0.558079 1.4:-30686604 MS.gene064950:CDS
TTAAACCCGGCTTTATAACC+AGG 0.558447 1.4:+30686473 None:intergenic
TTATATTTCCCAATGCGTAT+CGG 0.560807 1.4:-30686934 MS.gene064950:CDS
TACAAATCCAAATTCCTTGG+TGG 0.561392 1.4:+30687400 None:intergenic
AATAAGGCTTGCATTGATAA+CGG 0.564891 1.4:-30686578 MS.gene064950:CDS
GGTTGTCGAGAGTCTTGCTA+AGG 0.571851 1.4:-30687308 MS.gene064950:CDS
GTACACAGGTCTACCAAATG+AGG 0.572927 1.4:+30686670 None:intergenic
AGGTTACTCGTTTAATCGAA+CGG 0.577224 1.4:-30686780 MS.gene064950:CDS
AAACTATTCGGAAACAAATG+AGG 0.578354 1.4:+30687166 None:intergenic
GAAATGAATAAGATCATTGG+TGG 0.579179 1.4:-30687232 MS.gene064950:CDS
GCGGAGAGAATGGGAAACAT+AGG 0.580513 1.4:-30686800 MS.gene064950:CDS
GCCGGGTTTAAAGGTTGGGG+AGG 0.581968 1.4:-30686461 MS.gene064950:CDS
TTCGACAAATCACTCTGCTG+TGG 0.582757 1.4:+30687448 None:intergenic
GACATGTATCGCTCCTCCAA+AGG 0.585930 1.4:-30686395 MS.gene064950:CDS
AAGGGAATCCTGATCAGTAT+GGG 0.587521 1.4:-30687084 MS.gene064950:CDS
CGAAAGAGAAGCATTGTGAA+GGG 0.594406 1.4:-30687102 MS.gene064950:CDS
TTTGCGTCGTTTATTATCTG+TGG 0.594905 1.4:-30687617 MS.gene064950:CDS
TGTCGTTGAAGCATCATTGG+TGG 0.597927 1.4:-30687039 MS.gene064950:CDS
GCGAAAGAGAAGCATTGTGA+AGG 0.598171 1.4:-30687103 MS.gene064950:CDS
ACGCATTGGGAAATATAAAG+TGG 0.599578 1.4:+30686939 None:intergenic
ACCTCCCCAACCTTTAAACC+CGG 0.600678 1.4:+30686460 None:intergenic
CAGAAACTGAACTCTTACAA+GGG 0.604016 1.4:+30686362 None:intergenic
GTGGATGATGAACACGGTAT+GGG 0.605345 1.4:-30687017 MS.gene064950:CDS
TGAACTCGAGAGGTGAAGAG+TGG 0.605955 1.4:-30686835 MS.gene064950:CDS
TAATTGGGATATCACTATGT+GGG 0.611867 1.4:-30686705 MS.gene064950:CDS
ATAATTGGGATATCACTATG+TGG 0.612789 1.4:-30686706 MS.gene064950:CDS
AGAGTGGGATAGTTTAGTTG+CGG 0.613905 1.4:-30686819 MS.gene064950:CDS
ACGACACAATCTTCGGCGCA+CGG 0.615233 1.4:+30687056 None:intergenic
CCTCTCGAGTTCACATCAGA+AGG 0.618268 1.4:+30686845 None:intergenic
GAACTCGAGAGGTGAAGAGT+GGG 0.621084 1.4:-30686834 MS.gene064950:CDS
CCTTCTGATGTGAACTCGAG+AGG 0.621406 1.4:-30686845 MS.gene064950:CDS
TGGTTTGCATCAGGGTCAGG+GGG 0.622031 1.4:-30686603 MS.gene064950:CDS
CCACCTTAAGATACTGCGGC+CGG 0.633502 1.4:+30687329 None:intergenic
GGCTTGCATTGATAACGGCA+TGG 0.633628 1.4:-30686573 MS.gene064950:CDS
AGACCTGTGTACACGTTACG+AGG 0.633777 1.4:-30686659 MS.gene064950:CDS
ATGATGAACACGGTATGGGA+TGG 0.648748 1.4:-30687013 MS.gene064950:CDS
TTTGGAGGAGCGATACATGT+CGG 0.658581 1.4:+30686397 None:intergenic
TACACAGGTCTACCAAATGA+GGG 0.661524 1.4:+30686671 None:intergenic
TGTACACGTTACGAGGCCCA+AGG 0.664181 1.4:-30686652 MS.gene064950:CDS
CCGGCCGCAGTATCTTAAGG+TGG 0.691625 1.4:-30687329 MS.gene064950:CDS
GGGCCTCGTAACGTGTACAC+AGG 0.697516 1.4:+30686656 None:intergenic
ACAACCACCTTAAGATACTG+CGG 0.698025 1.4:+30687325 None:intergenic
CAAAGCTCAAGCATAAGTGG+CGG 0.706570 1.4:+30686420 None:intergenic
AAAGCCAAATCTCTAAGACG+TGG 0.718926 1.4:+30687637 None:intergenic
ACACAATCTTCGGCGCACGG+TGG 0.723175 1.4:+30687059 None:intergenic
TTGGTGGTGGATGATGAACA+CGG 0.805859 1.4:-30687023 MS.gene064950:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGTTATTGATATTTTTACT+TGG - chr1.4:30686460-30686479 MS.gene064950:CDS 15.0%
!!! TTTCTTTGATGATTATAATT+GGG - chr1.4:30687311-30687330 MS.gene064950:CDS 15.0%
!! TGAAACTAATGATTTAGAAA+GGG - chr1.4:30686504-30686523 MS.gene064950:CDS 20.0%
!! TTGAAACTAATGATTTAGAA+AGG - chr1.4:30686503-30686522 MS.gene064950:CDS 20.0%
!!! GTTTCTTTGATGATTATAAT+TGG - chr1.4:30687310-30687329 MS.gene064950:CDS 20.0%
! ATAGCATCTTTACCAATATT+TGG + chr1.4:30686658-30686677 None:intergenic 25.0%
! TTGGAAGAAAATACACAATA+AGG - chr1.4:30687275-30687294 MS.gene064950:CDS 25.0%
! TTGGATTTGTATCCAAATAT+TGG - chr1.4:30686643-30686662 MS.gene064950:CDS 25.0%
!!! TTGTTTTGTATGTTCATAAC+CGG - chr1.4:30686683-30686702 MS.gene064950:CDS 25.0%
AAACTATTCGGAAACAAATG+AGG + chr1.4:30686868-30686887 None:intergenic 30.0%
AAATTTGCAGCATAACAATC+AGG + chr1.4:30687162-30687181 None:intergenic 30.0%
ATAATTGGGATATCACTATG+TGG - chr1.4:30687325-30687344 MS.gene064950:CDS 30.0%
GAAATGAATAAGATCATTGG+TGG - chr1.4:30686799-30686818 MS.gene064950:CDS 30.0%
GAGGAAATGAATAAGATCAT+TGG - chr1.4:30686796-30686815 MS.gene064950:CDS 30.0%
TAATTGGGATATCACTATGT+GGG - chr1.4:30687326-30687345 MS.gene064950:CDS 30.0%
TTATATTTCCCAATGCGTAT+CGG - chr1.4:30687097-30687116 MS.gene064950:CDS 30.0%
! AATAAGGCTTGCATTGATAA+CGG - chr1.4:30687453-30687472 MS.gene064950:CDS 30.0%
! ATAAGATCATTGGTGGTATT+AGG - chr1.4:30686806-30686825 MS.gene064950:CDS 30.0%
! GAGTGATTTGTCGAAAAAAT+TGG - chr1.4:30686591-30686610 MS.gene064950:CDS 30.0%
!! TACGAACATTGATTCAGATT+GGG - chr1.4:30687512-30687531 MS.gene064950:CDS 30.0%
!! TTACGAACATTGATTCAGAT+TGG - chr1.4:30687511-30687530 MS.gene064950:CDS 30.0%
ACGCATTGGGAAATATAAAG+TGG + chr1.4:30687095-30687114 None:intergenic 35.0%
AGGTTACTCGTTTAATCGAA+CGG - chr1.4:30687251-30687270 MS.gene064950:CDS 35.0%
CATGTCTATAAAAATCAGCC+TGG - chr1.4:30687540-30687559 MS.gene064950:CDS 35.0%
GGATACAAATCCAAATTCCT+TGG + chr1.4:30686637-30686656 None:intergenic 35.0%
GTGGTGTTTCTTACTTTATG+TGG - chr1.4:30686433-30686452 MS.gene064950:CDS 35.0%
TAACAATCAGGACATTTCCT+AGG + chr1.4:30687150-30687169 None:intergenic 35.0%
TACAAATCCAAATTCCTTGG+TGG + chr1.4:30686634-30686653 None:intergenic 35.0%
TCGTGTAGAAGATCATGATA+AGG - chr1.4:30687488-30687507 MS.gene064950:CDS 35.0%
TGTAAATTCCGATACGCATT+GGG + chr1.4:30687108-30687127 None:intergenic 35.0%
TTTGCGTCGTTTATTATCTG+TGG - chr1.4:30686414-30686433 MS.gene064950:CDS 35.0%
! TGTTTCCGAATAGTTTTCCA+GGG - chr1.4:30686872-30686891 MS.gene064950:CDS 35.0%
! TTGTTTCCGAATAGTTTTCC+AGG - chr1.4:30686871-30686890 MS.gene064950:CDS 35.0%
!! GATTTAGAAAGGGTTGATGA+TGG - chr1.4:30686514-30686533 MS.gene064950:CDS 35.0%
!! TAGTCACGATTGTTACTTTG+AGG - chr1.4:30686777-30686796 MS.gene064950:CDS 35.0%
AAGGGAATCCTGATCAGTAT+GGG - chr1.4:30686947-30686966 MS.gene064950:CDS 40.0%
AATCAGCCTGGTTATAAAGC+CGG - chr1.4:30687552-30687571 MS.gene064950:CDS 40.0%
ACAACCACCTTAAGATACTG+CGG + chr1.4:30686709-30686728 None:intergenic 40.0%
ACTCGTTTAATCGAACGGTT+TGG - chr1.4:30687256-30687275 MS.gene064950:CDS 40.0%
AGAGTCTTGCTAAGGTTGTA+GGG - chr1.4:30686731-30686750 MS.gene064950:CDS 40.0%
AGAGTGGGATAGTTTAGTTG+CGG - chr1.4:30687212-30687231 MS.gene064950:CDS 40.0%
AGTTTAGTTGCGGAGAGAAT+GGG - chr1.4:30687222-30687241 MS.gene064950:CDS 40.0%
ATAAAGCCGGGTTTAAAGGT+TGG - chr1.4:30687565-30687584 MS.gene064950:CDS 40.0%
CTGTAAATTCCGATACGCAT+TGG + chr1.4:30687109-30687128 None:intergenic 40.0%
GAAACCACGTCTTAGAGATT+TGG - chr1.4:30686390-30686409 MS.gene064950:CDS 40.0%
GAAACCCCTGGAAAACTATT+CGG + chr1.4:30686880-30686899 None:intergenic 40.0%
GGAAACAAATGAGGCGAATA+CGG + chr1.4:30686859-30686878 None:intergenic 40.0%
GGTTATAAAGCCGGGTTTAA+AGG - chr1.4:30687561-30687580 MS.gene064950:CDS 40.0%
TAAAGCCGGGTTTAAAGGTT+GGG - chr1.4:30687566-30687585 MS.gene064950:CDS 40.0%
TACACAGGTCTACCAAATGA+GGG + chr1.4:30687363-30687382 None:intergenic 40.0%
TAGTTTAGTTGCGGAGAGAA+TGG - chr1.4:30687221-30687240 MS.gene064950:CDS 40.0%
TGCTTCAACGACACAATCTT+CGG + chr1.4:30686985-30687004 None:intergenic 40.0%
TTCTAACTTCGTTGAAGCCT+AGG - chr1.4:30687130-30687149 MS.gene064950:CDS 40.0%
TTTGTTGCCACCAAGGAATT+TGG - chr1.4:30686624-30686643 MS.gene064950:CDS 40.0%
! GGTTTCGATTGGTGATTGTA+AGG - chr1.4:30686894-30686913 MS.gene064950:CDS 40.0%
! GTTTCCGAATAGTTTTCCAG+GGG - chr1.4:30686873-30686892 MS.gene064950:CDS 40.0%
! TTAAACCCGGCTTTATAACC+AGG + chr1.4:30687561-30687580 None:intergenic 40.0%
! TTGTGTCGTTGAAGCATCAT+TGG - chr1.4:30686989-30687008 MS.gene064950:CDS 40.0%
!! AAAGCCAAATCTCTAAGACG+TGG + chr1.4:30686397-30686416 None:intergenic 40.0%
!! AGTTGAATTTGTTGCCACCA+AGG - chr1.4:30686617-30686636 MS.gene064950:CDS 40.0%
!! CGAAAGAGAAGCATTGTGAA+GGG - chr1.4:30686929-30686948 MS.gene064950:CDS 40.0%
AAAGCCGGGTTTAAAGGTTG+GGG - chr1.4:30687567-30687586 MS.gene064950:CDS 45.0%
AAGTGAACAGCACTAGTCCT+TGG + chr1.4:30687399-30687418 None:intergenic 45.0%
ACAAATCACTCTGCTGTGGA+AGG + chr1.4:30686582-30686601 None:intergenic 45.0%
AGTGAACAGCACTAGTCCTT+GGG + chr1.4:30687398-30687417 None:intergenic 45.0%
ATCAGCCTGGTTATAAAGCC+GGG - chr1.4:30687553-30687572 MS.gene064950:CDS 45.0%
ATGATGAACACGGTATGGGA+TGG - chr1.4:30687018-30687037 MS.gene064950:CDS 45.0%
CAAAGCTCAAGCATAAGTGG+CGG + chr1.4:30687614-30687633 None:intergenic 45.0%
CTTCAAATCGCGACGATAAC+CGG + chr1.4:30686542-30686561 None:intergenic 45.0%
GAAGGGAATCCTGATCAGTA+TGG - chr1.4:30686946-30686965 MS.gene064950:CDS 45.0%
GAGAGTCTTGCTAAGGTTGT+AGG - chr1.4:30686730-30686749 MS.gene064950:CDS 45.0%
GCGACTGTTTATCCCTCATT+TGG - chr1.4:30687348-30687367 MS.gene064950:CDS 45.0%
GTACACAGGTCTACCAAATG+AGG + chr1.4:30687364-30687383 None:intergenic 45.0%
GTGGATGATGAACACGGTAT+GGG - chr1.4:30687014-30687033 MS.gene064950:CDS 45.0%
GTTTAAAGGTTGGGGAGGTT+GGG - chr1.4:30687575-30687594 MS.gene064950:CDS 45.0%
TTCGACAAATCACTCTGCTG+TGG + chr1.4:30686586-30686605 None:intergenic 45.0%
TTTAAAGGTTGGGGAGGTTG+GGG - chr1.4:30687576-30687595 MS.gene064950:CDS 45.0%
TTTGGAGGAGCGATACATGT+CGG + chr1.4:30687637-30687656 None:intergenic 45.0%
! AGGACTAGTGCTGTTCACTT+TGG - chr1.4:30687399-30687418 MS.gene064950:CDS 45.0%
! AGTTTTCCAGGGGTTTCGAT+TGG - chr1.4:30686883-30686902 MS.gene064950:CDS 45.0%
! GCTGTTCACTTTGGGAAATG+TGG - chr1.4:30687408-30687427 MS.gene064950:CDS 45.0%
! GGACTAGTGCTGTTCACTTT+GGG - chr1.4:30687400-30687419 MS.gene064950:CDS 45.0%
! GGGAAATGTGGTTTGCATCA+GGG - chr1.4:30687420-30687439 MS.gene064950:CDS 45.0%
! TGGGAAATGTGGTTTGCATC+AGG - chr1.4:30687419-30687438 MS.gene064950:CDS 45.0%
! TGTCGTTGAAGCATCATTGG+TGG - chr1.4:30686992-30687011 MS.gene064950:CDS 45.0%
!! GAAAGGGTTGATGATGGTTC+CGG - chr1.4:30686520-30686539 MS.gene064950:CDS 45.0%
!! GCGAAAGAGAAGCATTGTGA+AGG - chr1.4:30686928-30686947 MS.gene064950:CDS 45.0%
!! TTGGTGGTGGATGATGAACA+CGG - chr1.4:30687008-30687027 MS.gene064950:CDS 45.0%
ACCTCCCCAACCTTTAAACC+CGG + chr1.4:30687574-30687593 None:intergenic 50.0%
AGACCTGTGTACACGTTACG+AGG - chr1.4:30687372-30687391 MS.gene064950:CDS 50.0%
CAAGGGAGATACAGTGCCTT+TGG + chr1.4:30687655-30687674 None:intergenic 50.0%
CAATCACCAATCGAAACCCC+TGG + chr1.4:30686892-30686911 None:intergenic 50.0%
CCTCTCGAGTTCACATCAGA+AGG + chr1.4:30687189-30687208 None:intergenic 50.0%
CCTTCTGATGTGAACTCGAG+AGG - chr1.4:30687186-30687205 MS.gene064950:CDS 50.0%
CGGCAAAGCTCAAGCATAAG+TGG + chr1.4:30687617-30687636 None:intergenic 50.0%
GAACTCGAGAGGTGAAGAGT+GGG - chr1.4:30687197-30687216 MS.gene064950:CDS 50.0%
GACATGTATCGCTCCTCCAA+AGG - chr1.4:30687636-30687655 MS.gene064950:CDS 50.0%
GCGGAGAGAATGGGAAACAT+AGG - chr1.4:30687231-30687250 MS.gene064950:CDS 50.0%
GGTGGATGATGAACACGGTA+TGG - chr1.4:30687013-30687032 MS.gene064950:CDS 50.0%
GGTTGTCGAGAGTCTTGCTA+AGG - chr1.4:30686723-30686742 MS.gene064950:CDS 50.0%
GGTTTAAAGGTTGGGGAGGT+TGG - chr1.4:30687574-30687593 MS.gene064950:CDS 50.0%
TAACCGGCCGCAGTATCTTA+AGG - chr1.4:30686699-30686718 MS.gene064950:CDS 50.0%
TGAACTCGAGAGGTGAAGAG+TGG - chr1.4:30687196-30687215 MS.gene064950:CDS 50.0%
TTAAAGGTTGGGGAGGTTGG+GGG - chr1.4:30687577-30687596 MS.gene064950:CDS 50.0%
! ATGTGGTTTGCATCAGGGTC+AGG - chr1.4:30687425-30687444 MS.gene064950:CDS 50.0%
! CGTTGAAGCATCATTGGTGG+TGG - chr1.4:30686995-30687014 MS.gene064950:CDS 50.0%
! TGTGGTTTGCATCAGGGTCA+GGG - chr1.4:30687426-30687445 MS.gene064950:CDS 50.0%
!! GGCTTGCATTGATAACGGCA+TGG - chr1.4:30687458-30687477 MS.gene064950:CDS 50.0%
ACGACACAATCTTCGGCGCA+CGG + chr1.4:30686978-30686997 None:intergenic 55.0%
CCACCTTAAGATACTGCGGC+CGG + chr1.4:30686705-30686724 None:intergenic 55.0%
CGGTGGTTCCCATACTGATC+AGG + chr1.4:30686958-30686977 None:intergenic 55.0%
GGGAGATACAGTGCCTTTGG+AGG + chr1.4:30687652-30687671 None:intergenic 55.0%
TAAAGGTTGGGGAGGTTGGG+GGG - chr1.4:30687578-30687597 MS.gene064950:CDS 55.0%
TCAGGGTCAGGGGGAGAATA+AGG - chr1.4:30687437-30687456 MS.gene064950:CDS 55.0%
TGTACACGTTACGAGGCCCA+AGG - chr1.4:30687379-30687398 MS.gene064950:CDS 55.0%
! AGTGAGACGAGAGCGCATTC+TGG - chr1.4:30687045-30687064 MS.gene064950:CDS 55.0%
! GTGAGACGAGAGCGCATTCT+GGG - chr1.4:30687046-30687065 MS.gene064950:CDS 55.0%
! GTGGTTTGCATCAGGGTCAG+GGG - chr1.4:30687427-30687446 MS.gene064950:CDS 55.0%
! TGGTTTGCATCAGGGTCAGG+GGG - chr1.4:30687428-30687447 MS.gene064950:CDS 55.0%
ACACAATCTTCGGCGCACGG+TGG + chr1.4:30686975-30686994 None:intergenic 60.0%
CCGGCCGCAGTATCTTAAGG+TGG - chr1.4:30686702-30686721 MS.gene064950:CDS 60.0%
GCCGGGTTTAAAGGTTGGGG+AGG - chr1.4:30687570-30687589 MS.gene064950:CDS 60.0%
GGGCCTCGTAACGTGTACAC+AGG + chr1.4:30687378-30687397 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 30686376 30687677 30686376 ID=MS.gene064950
chr1.4 mRNA 30686376 30687677 30686376 ID=MS.gene064950.t1;Parent=MS.gene064950
chr1.4 exon 30686376 30687677 30686376 ID=MS.gene064950.t1.exon1;Parent=MS.gene064950.t1
chr1.4 CDS 30686376 30687677 30686376 ID=cds.MS.gene064950.t1;Parent=MS.gene064950.t1
Gene Sequence

>MS.gene064950

ATGGCTTCTTATAAGAAACCACGTCTTAGAGATTTGGCTTTGCGTCGTTTATTATCTGTGGTGTTTCTTACTTTATGTGGTGTTTTGTTATTGATATTTTTACTTGGATCAAATTCAAGTACAACTATTGAAACTAATGATTTAGAAAGGGTTGATGATGGTTCCGGTTATCGTCGCGATTTGAAGTTTACTAAGTTGCAAAACCTTCCACAGCAGAGTGATTTGTCGAAAAAATTGGAAAAGTTGAATTTGTTGCCACCAAGGAATTTGGATTTGTATCCAAATATTGGTAAAGATGCTATCATTGTTGTTTTGTATGTTCATAACCGGCCGCAGTATCTTAAGGTGGTTGTCGAGAGTCTTGCTAAGGTTGTAGGGATTAATGAGACTTTATTGATTGTTAGTCACGATTGTTACTTTGAGGAAATGAATAAGATCATTGGTGGTATTAGGTTTTGTCAAGTTAAACAGATATATGCTCCGTATTCGCCTCATTTGTTTCCGAATAGTTTTCCAGGGGTTTCGATTGGTGATTGTAAGGAGAAAGATGATGCGAAAGAGAAGCATTGTGAAGGGAATCCTGATCAGTATGGGAACCACCGTGCGCCGAAGATTGTGTCGTTGAAGCATCATTGGTGGTGGATGATGAACACGGTATGGGATGGATTGAGTGAGACGAGAGCGCATTCTGGGCATGTTCTTTTTATTGAAGAAGACCACTTTATATTTCCCAATGCGTATCGGAATTTACAGATTCTAACTTCGTTGAAGCCTAGGAAATGTCCTGATTGTTATGCTGCAAATTTAGCACCTTCTGATGTGAACTCGAGAGGTGAAGAGTGGGATAGTTTAGTTGCGGAGAGAATGGGAAACATAGGTTACTCGTTTAATCGAACGGTTTGGAAGAAAATACACAATAAGGCTAAAGAATTTTGTTTCTTTGATGATTATAATTGGGATATCACTATGTGGGCGACTGTTTATCCCTCATTTGGTAGACCTGTGTACACGTTACGAGGCCCAAGGACTAGTGCTGTTCACTTTGGGAAATGTGGTTTGCATCAGGGTCAGGGGGAGAATAAGGCTTGCATTGATAACGGCATGGCGAACATTCGTGTAGAAGATCATGATAAGGTTACGAACATTGATTCAGATTGGGATGTTCATGTCTATAAAAATCAGCCTGGTTATAAAGCCGGGTTTAAAGGTTGGGGAGGTTGGGGGGATGACAGAGACCGCCACTTATGCTTGAGCTTTGCCGACATGTATCGCTCCTCCAAAGGCACTGTATCTCCCTTGTAA
Protein sequence

>MS.gene064950.t1

MASYKKPRLRDLALRRLLSVVFLTLCGVLLLIFLLGSNSSTTIETNDLERVDDGSGYRRDLKFTKLQNLPQQSDLSKKLEKLNLLPPRNLDLYPNIGKDAIIVVLYVHNRPQYLKVVVESLAKVVGINETLLIVSHDCYFEEMNKIIGGIRFCQVKQIYAPYSPHLFPNSFPGVSIGDCKEKDDAKEKHCEGNPDQYGNHRAPKIVSLKHHWWWMMNTVWDGLSETRAHSGHVLFIEEDHFIFPNAYRNLQILTSLKPRKCPDCYAANLAPSDVNSRGEEWDSLVAERMGNIGYSFNRTVWKKIHNKAKEFCFFDDYNWDITMWATVYPSFGRPVYTLRGPRTSAVHFGKCGLHQGQGENKACIDNGMANIRVEDHDKVTNIDSDWDVHVYKNQPGYKAGFKGWGGWGDDRDRHLCLSFADMYRSSKGTVSPL