AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065001

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065001.t1 MTR_1g112860 97.909 287 6 0 1 287 1 287 0.0 564
MS.gene065001.t1 MTR_1g112860 94.077 287 6 1 1 287 1 276 0.0 533
MS.gene065001.t1 MTR_4g112540 24.215 223 142 4 34 250 15 216 4.11e-13 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065001.t1 AT2G20920 62.245 294 94 6 3 287 2 287 1.96e-113 328

Find 87 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATAATCATCTCTCAGTTCT+TGG 0.239865 1.2:-83626541 MS.gene065001:CDS
CTCGTAGCCGGTGCTGTTGT+AGG 0.250905 1.2:-83626645 MS.gene065001:CDS
GTAGATATTGTCCCATTTCT+AGG 0.260172 1.2:-83626597 MS.gene065001:CDS
TTATGCTGGGGCTGATGTTC+CGG 0.280484 1.2:-83627922 MS.gene065001:CDS
CTTATCTTGGCTGGTAGCTT+TGG 0.288436 1.2:-83627897 MS.gene065001:CDS
GGTCTACAAGCTGGAGTATA+TGG 0.336678 1.2:-83627996 MS.gene065001:CDS
TGATGTTCCGGGACTTATCT+TGG 0.337818 1.2:-83627910 MS.gene065001:CDS
CGAAGAAGAGCCACTAAAAT+AGG 0.339775 1.2:+83627956 None:intergenic
CTGCGAACCGGTGGTGGTCT+TGG 0.339944 1.2:+83628754 None:intergenic
GAGGAGCGTGGGACCGGTTC+AGG 0.342637 1.2:-83628668 MS.gene065001:CDS
TGAAACTGGTGCTTTCTTCA+TGG 0.383086 1.2:+83628046 None:intergenic
TATGCTGGGGCTGATGTTCC+GGG 0.398811 1.2:-83627921 MS.gene065001:CDS
TGCGAACCGGTGGTGGTCTT+GGG 0.401013 1.2:+83628755 None:intergenic
TAACCTGAGAAATAGTTTCT+TGG 0.402696 1.2:+83628506 None:intergenic
GGGGAGCGTATCCCTAGAAA+TGG 0.412971 1.2:+83626585 None:intergenic
AACCGTGGCCGCTACGTAGA+AGG 0.413617 1.2:-83628708 MS.gene065001:CDS
TCACCTTCCACCAGTGATCT+TGG 0.418919 1.2:-83628017 MS.gene065001:CDS
AAGGCTGTTGTTATAACAAT+AGG 0.421906 1.2:-83626672 MS.gene065001:CDS
GCGCGCGAACTCGCCTGAAC+CGG 0.436685 1.2:+83628655 None:intergenic
ACTTCGCTAACAACAGCAGC+TGG 0.463530 1.2:+83626564 None:intergenic
TCTACGTAGCGGCCACGGTT+CGG 0.469629 1.2:+83628711 None:intergenic
TAGGGTCAGTTGTAGAGAAC+TGG 0.471767 1.2:-83626626 MS.gene065001:CDS
GAAAACGCACTGAAGCTGTT+AGG 0.474719 1.2:-83628601 MS.gene065001:CDS
GTTCCGGGACTTATCTTGGC+TGG 0.475158 1.2:-83627906 MS.gene065001:CDS
CGACCAAGAAACTATTTCTC+AGG 0.482341 1.2:-83628509 MS.gene065001:intron
TCGTAGCCGGTGCTGTTGTA+GGG 0.487487 1.2:-83626644 MS.gene065001:CDS
ATCTTTCCCAAGACCACCAC+CGG 0.487703 1.2:-83628761 MS.gene065001:CDS
GTCAACGAGGAGCGTGGGAC+CGG 0.488203 1.2:-83628674 MS.gene065001:CDS
AGGTTAACTCAGCGGCGAGC+AGG 0.492386 1.2:-83628158 MS.gene065001:CDS
AAGATGAACCGGCAGAGATA+CGG 0.494662 1.2:+83628779 None:intergenic
GAAGGTGACTCAATTGAAAC+TGG 0.499493 1.2:+83628032 None:intergenic
GAGATACGGTCGGACCGGAC+GGG 0.499884 1.2:+83628793 None:intergenic
GAGGCATTGAACTATTAGCC+TGG 0.508666 1.2:+83628084 None:intergenic
CTAATAGTTCAATGCCTCAA+TGG 0.518261 1.2:-83628079 MS.gene065001:CDS
TGTAAAACGTGTTCAGTCCC+AGG 0.519566 1.2:-83628102 MS.gene065001:CDS
TAGATATTGTCCCATTTCTA+GGG 0.520925 1.2:-83626596 MS.gene065001:CDS
CTCAGCACCGTTCGAGATGT+CGG 0.522136 1.2:-83628626 MS.gene065001:CDS
TCTTGGTTTCGCTATACCTA+AGG 0.523449 1.2:-83626524 MS.gene065001:CDS
TGGTCTTGGGAAAGATGAAC+CGG 0.528342 1.2:+83628768 None:intergenic
ACTGAAGCTGTTAGGTGTAT+CGG 0.529175 1.2:-83628593 MS.gene065001:CDS
CGGTCCGACCGTATCTCTGC+CGG 0.529633 1.2:-83628787 MS.gene065001:CDS
TCAGCCCCAGCATAACCTGC+AGG 0.529775 1.2:+83627930 None:intergenic
GCTGTTGTTATAACAATAGG+AGG 0.530149 1.2:-83626669 MS.gene065001:CDS
GACCGGACGGGGATAGTAGC+TGG 0.530833 1.2:+83628805 None:intergenic
AACTGGTGCTTTCTTCATGG+TGG 0.533216 1.2:+83628049 None:intergenic
ACGAGACTGCGAACCGGTGG+TGG 0.533492 1.2:+83628748 None:intergenic
ACAATAGGAGGGCTCGTAGC+CGG 0.533690 1.2:-83626657 MS.gene065001:CDS
CAGCCCCAGCATAACCTGCA+GGG 0.534202 1.2:+83627931 None:intergenic
AGTGATCTTGGTCTACAAGC+TGG 0.534395 1.2:-83628005 MS.gene065001:CDS
CACTCGTCAACGAGGAGCGT+GGG 0.536752 1.2:-83628679 MS.gene065001:CDS
CGGCAGAGATACGGTCGGAC+CGG 0.541076 1.2:+83628788 None:intergenic
GGCGCGAAGTATATCATCAA+AGG 0.548559 1.2:+83628561 None:intergenic
AGAGATACGGTCGGACCGGA+CGG 0.551833 1.2:+83628792 None:intergenic
TGGAGTATATGGTGCACTGA+TGG 0.556326 1.2:-83627985 MS.gene065001:CDS
CTTCGCTAACAACAGCAGCT+GGG 0.557128 1.2:+83626565 None:intergenic
AGGCATTGAACTATTAGCCT+GGG 0.559812 1.2:+83628085 None:intergenic
GGGAGCGTATCCCTAGAAAT+GGG 0.559951 1.2:+83626586 None:intergenic
GGCCACGGTTCGGTGAGGAT+CGG 0.561057 1.2:+83628721 None:intergenic
CACCTTCTACGTAGCGGCCA+CGG 0.561943 1.2:+83628706 None:intergenic
CACCAGCTACTATCCCCGTC+CGG 0.566811 1.2:-83628807 MS.gene065001:CDS
AGCTTGTAGACCAAGATCAC+TGG 0.567895 1.2:+83628007 None:intergenic
AGATACGGTCGGACCGGACG+GGG 0.570930 1.2:+83628794 None:intergenic
AGTTGTAGAGAACTGGCTGC+AGG 0.574354 1.2:-83626619 MS.gene065001:CDS
CTACCAGCCAAGATAAGTCC+CGG 0.574753 1.2:+83627903 None:intergenic
AACTGACCCTACAACAGCAC+CGG 0.576918 1.2:+83626638 None:intergenic
AGCCCCAGCATAACCTGCAG+GGG 0.587242 1.2:+83627932 None:intergenic
GGAGTATATGGTGCACTGAT+GGG 0.588659 1.2:-83627984 MS.gene065001:CDS
GACGGGGATAGTAGCTGGTG+TGG 0.596353 1.2:+83628810 None:intergenic
GGTTTAACGAGACTGCGAAC+CGG 0.597083 1.2:+83628742 None:intergenic
TTCGCTAACAACAGCAGCTG+GGG 0.602984 1.2:+83626566 None:intergenic
GCACTCGTCAACGAGGAGCG+TGG 0.612531 1.2:-83628680 MS.gene065001:CDS
GTCTTTGCAAGAAGCAACGA+TGG 0.615598 1.2:+83628531 None:intergenic
GCCCCAGCATAACCTGCAGG+GGG 0.620500 1.2:+83627933 None:intergenic
ATGACATGTTGCTTATGCAG+AGG 0.621680 1.2:-83628178 MS.gene065001:CDS
CGTGGCCGCTACGTAGAAGG+TGG 0.636433 1.2:-83628705 MS.gene065001:CDS
ATGGTGGATTGCATCCATTG+AGG 0.641012 1.2:+83628065 None:intergenic
TTGTAGACCAAGATCACTGG+TGG 0.661997 1.2:+83628010 None:intergenic
AGACCAAGATCACTGGTGGA+AGG 0.664781 1.2:+83628014 None:intergenic
AACCGATCCTCACCGAACCG+TGG 0.674182 1.2:-83628723 MS.gene065001:CDS
CTGTTGTTATAACAATAGGA+GGG 0.684835 1.2:-83626668 MS.gene065001:CDS
AAGCTGTTAGGTGTATCGGA+AGG 0.687616 1.2:-83628589 MS.gene065001:CDS
TTAACGAGACTGCGAACCGG+TGG 0.689656 1.2:+83628745 None:intergenic
GTAGCGGCCACGGTTCGGTG+AGG 0.697717 1.2:+83628716 None:intergenic
TTATGCAGAGGTTAACTCAG+CGG 0.705557 1.2:-83628166 MS.gene065001:CDS
TGCTTCCACCTTCTACGTAG+CGG 0.712698 1.2:+83628700 None:intergenic
GGTGGAAGCACTCGTCAACG+AGG 0.726269 1.2:-83628687 MS.gene065001:CDS
TGAACCGGCAGAGATACGGT+CGG 0.737243 1.2:+83628783 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTTTTGATATTAAAAGTTT+TGG - chr1.2:83628076-83628095 MS.gene065001:CDS 15.0%
!! AAGCTAAAAATCAATTCTAA+AGG + chr1.2:83628453-83628472 None:intergenic 20.0%
!! AAGTTGTTTATCTTAACAAA+TGG - chr1.2:83628209-83628228 MS.gene065001:intron 20.0%
!! AATAAAAGTAAGCATTTAGA+TGG - chr1.2:83627594-83627613 MS.gene065001:intron 20.0%
!! ATTATGAATGTCTACAAAAT+TGG + chr1.2:83627936-83627955 None:intergenic 20.0%
!!! AACTTTTAATATCAAAAGTC+TGG + chr1.2:83628075-83628094 None:intergenic 20.0%
!!! ACTTTTAATATCAAAAGTCT+GGG + chr1.2:83628074-83628093 None:intergenic 20.0%
!!! TTAACTTGAAGATAGAATTT+GGG - chr1.2:83628420-83628439 MS.gene065001:intron 20.0%
!!! TTTAACTTGAAGATAGAATT+TGG - chr1.2:83628419-83628438 MS.gene065001:intron 20.0%
!!! TTTTGTAGACATTCATAATA+AGG - chr1.2:83627937-83627956 MS.gene065001:CDS 20.0%
! AAAGAATGTTAAGTTAGGTA+AGG - chr1.2:83627488-83627507 MS.gene065001:intron 25.0%
! AAATGTACAGTTTCAATAAG+AGG + chr1.2:83627836-83627855 None:intergenic 25.0%
! AATGTACAGTTTCAATAAGA+GGG + chr1.2:83627835-83627854 None:intergenic 25.0%
! ACATTGATATTGTAATGCAA+TGG - chr1.2:83627524-83627543 MS.gene065001:intron 25.0%
! ACCAAAAAGAATGTTAAGTT+AGG - chr1.2:83627483-83627502 MS.gene065001:intron 25.0%
! AGAAATGAAGTCTATTCAAT+AGG + chr1.2:83628043-83628062 None:intergenic 25.0%
! CAAAATCAATTATACACCTT+TGG + chr1.2:83628350-83628369 None:intergenic 25.0%
! GCTTATATTATAGCTTATAC+AGG + chr1.2:83626951-83626970 None:intergenic 25.0%
! TAACTTGAAGATAGAATTTG+GGG - chr1.2:83628421-83628440 MS.gene065001:intron 25.0%
! TAATTCTATACACCTATTGA+TGG - chr1.2:83627105-83627124 MS.gene065001:intron 25.0%
! TAGTTTAAGAAAGAGGTAAA+CGG + chr1.2:83628281-83628300 None:intergenic 25.0%
!! ACCTAACTTAACATTCTTTT+TGG + chr1.2:83627487-83627506 None:intergenic 25.0%
!! CTTCAAGTTAAAATAAATCC+TGG + chr1.2:83628411-83628430 None:intergenic 25.0%
!! TAGAACTTTTCTTTCAACAA+AGG + chr1.2:83627685-83627704 None:intergenic 25.0%
!!! AGGGTTTTATTTACCTAATA+TGG - chr1.2:83628150-83628169 MS.gene065001:CDS 25.0%
!!! ATAATTGATTTTGGCATGTT+TGG - chr1.2:83628357-83628376 MS.gene065001:intron 25.0%
!!! CAAAGGTGTATAATTGATTT+TGG - chr1.2:83628348-83628367 MS.gene065001:intron 25.0%
!!! GAGATTGTTTCTGTATAAAA+TGG - chr1.2:83628172-83628191 MS.gene065001:CDS 25.0%
AAAGCTATTCAGATGAAAGT+AGG + chr1.2:83628492-83628511 None:intergenic 30.0%
AAATGCTATGCTAATCCAAA+AGG + chr1.2:83628591-83628610 None:intergenic 30.0%
AAGGCTGTTGTTATAACAAT+AGG - chr1.2:83628666-83628685 MS.gene065001:CDS 30.0%
ACAGAAACAATCTCCATATT+AGG + chr1.2:83628166-83628185 None:intergenic 30.0%
ATGAAGTCTATTCAATAGGT+TGG + chr1.2:83628039-83628058 None:intergenic 30.0%
ATGTACAGTTTCAATAAGAG+GGG + chr1.2:83627834-83627853 None:intergenic 30.0%
CAAATCAACAACAAGTAAAG+TGG + chr1.2:83626860-83626879 None:intergenic 30.0%
CAAGTTAAAATAAATCCTGG+AGG + chr1.2:83628408-83628427 None:intergenic 30.0%
CATTCTTTGTCGAAACAATA+AGG - chr1.2:83628013-83628032 MS.gene065001:CDS 30.0%
CCAAAAGGATTAAGTGTATT+TGG + chr1.2:83628576-83628595 None:intergenic 30.0%
CCATTAGAACTTTAACCAAT+AGG - chr1.2:83627780-83627799 MS.gene065001:intron 30.0%
CTGAGAATAGTTTAAGAAAG+AGG + chr1.2:83628288-83628307 None:intergenic 30.0%
CTGTTGTTATAACAATAGGA+GGG - chr1.2:83628670-83628689 MS.gene065001:CDS 30.0%
CTTATTGTTTCGACAAAGAA+TGG + chr1.2:83628015-83628034 None:intergenic 30.0%
TAACCTGAGAAATAGTTTCT+TGG + chr1.2:83626835-83626854 None:intergenic 30.0%
TACATGAATTTCAAGACCAT+GGG + chr1.2:83627015-83627034 None:intergenic 30.0%
TAGATATTGTCCCATTTCTA+GGG - chr1.2:83628742-83628761 MS.gene065001:CDS 30.0%
TCAGTTTCACTAAAGTTATG+TGG - chr1.2:83627739-83627758 MS.gene065001:intron 30.0%
! ATAGAAAAGGGAAGTGTAAA+AGG + chr1.2:83628636-83628655 None:intergenic 30.0%
! CCAAATACACTTAATCCTTT+TGG - chr1.2:83628573-83628592 MS.gene065001:CDS 30.0%
! CCTATTGGTTAAAGTTCTAA+TGG + chr1.2:83627783-83627802 None:intergenic 30.0%
! TCTAATGGATCTAATTCATG+TGG + chr1.2:83627768-83627787 None:intergenic 30.0%
! TGGAATTTCTGTGCATTTTT+AGG - chr1.2:83627125-83627144 MS.gene065001:intron 30.0%
!! ATTCGTATTGACTTGTATCA+TGG - chr1.2:83627571-83627590 MS.gene065001:intron 30.0%
!! TGGAATTTTTGTTTCATGAG+TGG - chr1.2:83627544-83627563 MS.gene065001:intron 30.0%
AACTAGAGACTGAAATTGTG+AGG + chr1.2:83627061-83627080 None:intergenic 35.0%
AAGCATTTAGATGGCCATAA+TGG - chr1.2:83627603-83627622 MS.gene065001:intron 35.0%
AGCATTTAGATGGCCATAAT+GGG - chr1.2:83627604-83627623 MS.gene065001:intron 35.0%
CATAATCATCTCTCAGTTCT+TGG - chr1.2:83628797-83628816 MS.gene065001:CDS 35.0%
CTAATAGTTCAATGCCTCAA+TGG - chr1.2:83627259-83627278 MS.gene065001:intron 35.0%
CTTTAACCAATAGGAGATAG+TGG - chr1.2:83627789-83627808 MS.gene065001:intron 35.0%
GCTGTTGTTATAACAATAGG+AGG - chr1.2:83628669-83628688 MS.gene065001:CDS 35.0%
GTACATGAATTTCAAGACCA+TGG + chr1.2:83627016-83627035 None:intergenic 35.0%
GTAGATATTGTCCCATTTCT+AGG - chr1.2:83628741-83628760 MS.gene065001:CDS 35.0%
TGCACAGAAATTCCATCAAT+AGG + chr1.2:83627120-83627139 None:intergenic 35.0%
TTCAACAAAGGCAAACATGA+AGG + chr1.2:83627673-83627692 None:intergenic 35.0%
TTCCCTGCAGAAATAGAAAA+GGG + chr1.2:83628648-83628667 None:intergenic 35.0%
TTCTAACCACTATCTCCTAT+TGG + chr1.2:83627798-83627817 None:intergenic 35.0%
TTCTTATACTGCTAATCTGC+TGG - chr1.2:83628101-83628120 MS.gene065001:CDS 35.0%
! ACAAATGGTACTTCTAGTTG+TGG - chr1.2:83628224-83628243 MS.gene065001:intron 35.0%
! ACTGAAATTGTGAGGTATTC+AGG + chr1.2:83627053-83627072 None:intergenic 35.0%
! CAAATGGTACTTCTAGTTGT+GGG - chr1.2:83628225-83628244 MS.gene065001:intron 35.0%
! TTGGCATGTTTGGATGTTTT+CGG - chr1.2:83628367-83628386 MS.gene065001:intron 35.0%
!! ATAGAATTGATTTTGCCTCC+AGG - chr1.2:83628390-83628409 MS.gene065001:intron 35.0%
!! ATGGGTTTCACCTATTTTAG+TGG - chr1.2:83627372-83627391 MS.gene065001:intron 35.0%
!! ATTAAGTGTATTTGGCTCTG+CGG + chr1.2:83628568-83628587 None:intergenic 35.0%
!! CTTTTTGGTCATGAAGTACA+AGG + chr1.2:83627472-83627491 None:intergenic 35.0%
!!! TACACCTTTGGAGTCAATTT+TGG + chr1.2:83628338-83628357 None:intergenic 35.0%
!!! TTCCCTTTTCTATTTCTGCA+GGG - chr1.2:83628643-83628662 MS.gene065001:CDS 35.0%
AATCTGCTGGATATTACTGC+TGG - chr1.2:83628114-83628133 MS.gene065001:CDS 40.0%
ACTGAAGCTGTTAGGTGTAT+CGG - chr1.2:83626745-83626764 MS.gene065001:intron 40.0%
CGAAGAAGAGCCACTAAAAT+AGG + chr1.2:83627385-83627404 None:intergenic 40.0%
CTTCCCTGCAGAAATAGAAA+AGG + chr1.2:83628649-83628668 None:intergenic 40.0%
GAAGGTGACTCAATTGAAAC+TGG + chr1.2:83627309-83627328 None:intergenic 40.0%
TCTTGGTTTCGCTATACCTA+AGG - chr1.2:83628814-83628833 MS.gene065001:CDS 40.0%
TGAAACTGGTGCTTTCTTCA+TGG + chr1.2:83627295-83627314 None:intergenic 40.0%
TGAAGACACAAAGCAATGAG+TGG - chr1.2:83627871-83627890 MS.gene065001:CDS 40.0%
TTATGCAGAGGTTAACTCAG+CGG - chr1.2:83627172-83627191 MS.gene065001:intron 40.0%
! AGAAGCAACGATGGAGTTTT+TGG + chr1.2:83626801-83626820 None:intergenic 40.0%
! AGAGCCAAAATTGACTCCAA+AGG - chr1.2:83628331-83628350 MS.gene065001:intron 40.0%
! ATGACATGTTGCTTATGCAG+AGG - chr1.2:83627160-83627179 MS.gene065001:intron 40.0%
! ATTTAGATGGCCATAATGGG+TGG - chr1.2:83627607-83627626 MS.gene065001:intron 40.0%
! CGACCAAGAAACTATTTCTC+AGG - chr1.2:83626829-83626848 MS.gene065001:intron 40.0%
! GAAAAGGGAAGTGTAAAAGG+AGG + chr1.2:83628633-83628652 None:intergenic 40.0%
! TGAGGTATTCAGGACATCAA+GGG + chr1.2:83627043-83627062 None:intergenic 40.0%
! TTTTCAACCGACATCTCGAA+CGG + chr1.2:83626722-83626741 None:intergenic 40.0%
!! AGGCATTGAACTATTAGCCT+GGG + chr1.2:83627256-83627275 None:intergenic 40.0%
!! TTTAGATGGCCATAATGGGT+GGG - chr1.2:83627608-83627627 MS.gene065001:intron 40.0%
!!! ATGCTTGAGTTTTTTGCCCA+TGG - chr1.2:83626996-83627015 MS.gene065001:intron 40.0%
!!! CTTCCCTTTTCTATTTCTGC+AGG - chr1.2:83628642-83628661 MS.gene065001:CDS 40.0%
!!! CTTTTCTATTTCTGCAGGGA+AGG - chr1.2:83628647-83628666 MS.gene065001:CDS 40.0%
!!! TTCTGTGCATTTTTAGGTCG+AGG - chr1.2:83627131-83627150 MS.gene065001:intron 40.0%
AACTGGTGCTTTCTTCATGG+TGG + chr1.2:83627292-83627311 None:intergenic 45.0%
AAGATGAACCGGCAGAGATA+CGG + chr1.2:83626562-83626581 None:intergenic 45.0%
AAGCTGTTAGGTGTATCGGA+AGG - chr1.2:83626749-83626768 MS.gene065001:intron 45.0%
AGCTTGTAGACCAAGATCAC+TGG + chr1.2:83627334-83627353 None:intergenic 45.0%
AGTGATCTTGGTCTACAAGC+TGG - chr1.2:83627333-83627352 MS.gene065001:intron 45.0%
CTTATCTTGGCTGGTAGCTT+TGG - chr1.2:83627441-83627460 MS.gene065001:intron 45.0%
GAAAACGCACTGAAGCTGTT+AGG - chr1.2:83626737-83626756 MS.gene065001:intron 45.0%
GGCGCGAAGTATATCATCAA+AGG + chr1.2:83626780-83626799 None:intergenic 45.0%
GGTCTACAAGCTGGAGTATA+TGG - chr1.2:83627342-83627361 MS.gene065001:intron 45.0%
GTCTTTGCAAGAAGCAACGA+TGG + chr1.2:83626810-83626829 None:intergenic 45.0%
TAGGGTCAGTTGTAGAGAAC+TGG - chr1.2:83628712-83628731 MS.gene065001:CDS 45.0%
TGATGTTCCGGGACTTATCT+TGG - chr1.2:83627428-83627447 MS.gene065001:intron 45.0%
TGGAGTATATGGTGCACTGA+TGG - chr1.2:83627353-83627372 MS.gene065001:intron 45.0%
TGTAAAACGTGTTCAGTCCC+AGG - chr1.2:83627236-83627255 MS.gene065001:intron 45.0%
TTGGTGCACTGTATGATGCA+AGG + chr1.2:83627917-83627936 None:intergenic 45.0%
TTGTAGACCAAGATCACTGG+TGG + chr1.2:83627331-83627350 None:intergenic 45.0%
! ATGGTACTTCTAGTTGTGGG+TGG - chr1.2:83628228-83628247 MS.gene065001:intron 45.0%
! GAGGTATTCAGGACATCAAG+GGG + chr1.2:83627042-83627061 None:intergenic 45.0%
! GGAGTATATGGTGCACTGAT+GGG - chr1.2:83627354-83627373 MS.gene065001:intron 45.0%
! GTGAGGTATTCAGGACATCA+AGG + chr1.2:83627044-83627063 None:intergenic 45.0%
! TGGTCTTGGGAAAGATGAAC+CGG + chr1.2:83626573-83626592 None:intergenic 45.0%
!! ATGGTGGATTGCATCCATTG+AGG + chr1.2:83627276-83627295 None:intergenic 45.0%
!! GAGGCATTGAACTATTAGCC+TGG + chr1.2:83627257-83627276 None:intergenic 45.0%
!! TGCTGGTGTCTTTGAAGTGA+GGG - chr1.2:83628131-83628150 MS.gene065001:CDS 45.0%
AACTGACCCTACAACAGCAC+CGG + chr1.2:83628703-83628722 None:intergenic 50.0%
ACTTCGCTAACAACAGCAGC+TGG + chr1.2:83628777-83628796 None:intergenic 50.0%
AGACCAAGATCACTGGTGGA+AGG + chr1.2:83627327-83627346 None:intergenic 50.0%
AGTTGTAGAGAACTGGCTGC+AGG - chr1.2:83628719-83628738 MS.gene065001:CDS 50.0%
ATCTTTCCCAAGACCACCAC+CGG - chr1.2:83626577-83626596 MS.gene065001:CDS 50.0%
CTACCAGCCAAGATAAGTCC+CGG + chr1.2:83627438-83627457 None:intergenic 50.0%
CTTCGCTAACAACAGCAGCT+GGG + chr1.2:83628776-83628795 None:intergenic 50.0%
GGTTTAACGAGACTGCGAAC+CGG + chr1.2:83626599-83626618 None:intergenic 50.0%
TCACCTTCCACCAGTGATCT+TGG - chr1.2:83627321-83627340 MS.gene065001:intron 50.0%
TCCTACTCTCCCACCCATTA+TGG + chr1.2:83627620-83627639 None:intergenic 50.0%
TGCTTCCACCTTCTACGTAG+CGG + chr1.2:83626641-83626660 None:intergenic 50.0%
TTATGCTGGGGCTGATGTTC+CGG - chr1.2:83627416-83627435 MS.gene065001:intron 50.0%
TTCGCTAACAACAGCAGCTG+GGG + chr1.2:83628775-83628794 None:intergenic 50.0%
! GGGAGCGTATCCCTAGAAAT+GGG + chr1.2:83628755-83628774 None:intergenic 50.0%
!! CTGCTGGTGTCTTTGAAGTG+AGG - chr1.2:83628130-83628149 MS.gene065001:CDS 50.0%
ACAATAGGAGGGCTCGTAGC+CGG - chr1.2:83628681-83628700 MS.gene065001:CDS 55.0%
CTCAGCACCGTTCGAGATGT+CGG - chr1.2:83626712-83626731 MS.gene065001:intron 55.0%
GTTCCGGGACTTATCTTGGC+TGG - chr1.2:83627432-83627451 MS.gene065001:intron 55.0%
TATGCTGGGGCTGATGTTCC+GGG - chr1.2:83627417-83627436 MS.gene065001:intron 55.0%
TGAACCGGCAGAGATACGGT+CGG + chr1.2:83626558-83626577 None:intergenic 55.0%
TTAACGAGACTGCGAACCGG+TGG + chr1.2:83626596-83626615 None:intergenic 55.0%
! GGGGAGCGTATCCCTAGAAA+TGG + chr1.2:83628756-83628775 None:intergenic 55.0%
!! GCCATAATGGGTGGGAGAGT+AGG - chr1.2:83627616-83627635 MS.gene065001:intron 55.0%
!! TCGTAGCCGGTGCTGTTGTA+GGG - chr1.2:83628694-83628713 MS.gene065001:CDS 55.0%
AACCGATCCTCACCGAACCG+TGG - chr1.2:83626615-83626634 MS.gene065001:CDS 60.0%
AACCGTGGCCGCTACGTAGA+AGG - chr1.2:83626630-83626649 MS.gene065001:CDS 60.0%
AGAGATACGGTCGGACCGGA+CGG + chr1.2:83626549-83626568 None:intergenic 60.0%
AGCCCCAGCATAACCTGCAG+GGG + chr1.2:83627409-83627428 None:intergenic 60.0%
AGGTTAACTCAGCGGCGAGC+AGG - chr1.2:83627180-83627199 MS.gene065001:intron 60.0%
CACCAGCTACTATCCCCGTC+CGG - chr1.2:83626531-83626550 MS.gene065001:CDS 60.0%
CACCTTCTACGTAGCGGCCA+CGG + chr1.2:83626635-83626654 None:intergenic 60.0%
CAGCCCCAGCATAACCTGCA+GGG + chr1.2:83627410-83627429 None:intergenic 60.0%
GACGGGGATAGTAGCTGGTG+TGG + chr1.2:83626531-83626550 None:intergenic 60.0%
TCAGCCCCAGCATAACCTGC+AGG + chr1.2:83627411-83627430 None:intergenic 60.0%
TCTACGTAGCGGCCACGGTT+CGG + chr1.2:83626630-83626649 None:intergenic 60.0%
! CACTCGTCAACGAGGAGCGT+GGG - chr1.2:83626659-83626678 MS.gene065001:CDS 60.0%
! TGCGAACCGGTGGTGGTCTT+GGG + chr1.2:83626586-83626605 None:intergenic 60.0%
!! CTCGTAGCCGGTGCTGTTGT+AGG - chr1.2:83628693-83628712 MS.gene065001:CDS 60.0%
!! GGTGGAAGCACTCGTCAACG+AGG - chr1.2:83626651-83626670 MS.gene065001:CDS 60.0%
ACGAGACTGCGAACCGGTGG+TGG + chr1.2:83626593-83626612 None:intergenic 65.0%
AGATACGGTCGGACCGGACG+GGG + chr1.2:83626547-83626566 None:intergenic 65.0%
CGCCCCCTGCAGGTTATGCT+GGG - chr1.2:83627403-83627422 MS.gene065001:intron 65.0%
CGGCAGAGATACGGTCGGAC+CGG + chr1.2:83626553-83626572 None:intergenic 65.0%
CGGTCCGACCGTATCTCTGC+CGG - chr1.2:83626551-83626570 MS.gene065001:CDS 65.0%
CGTGGCCGCTACGTAGAAGG+TGG - chr1.2:83626633-83626652 MS.gene065001:CDS 65.0%
GACCGGACGGGGATAGTAGC+TGG + chr1.2:83626536-83626555 None:intergenic 65.0%
GAGATACGGTCGGACCGGAC+GGG + chr1.2:83626548-83626567 None:intergenic 65.0%
GCACTCGTCAACGAGGAGCG+TGG - chr1.2:83626658-83626677 MS.gene065001:CDS 65.0%
GCCCCAGCATAACCTGCAGG+GGG + chr1.2:83627408-83627427 None:intergenic 65.0%
GCCCCCTGCAGGTTATGCTG+GGG - chr1.2:83627404-83627423 MS.gene065001:intron 65.0%
TCGCCCCCTGCAGGTTATGC+TGG - chr1.2:83627402-83627421 MS.gene065001:intron 65.0%
! CTGCGAACCGGTGGTGGTCT+TGG + chr1.2:83626587-83626606 None:intergenic 65.0%
! GTCAACGAGGAGCGTGGGAC+CGG - chr1.2:83626664-83626683 MS.gene065001:CDS 65.0%
!! GGCCACGGTTCGGTGAGGAT+CGG + chr1.2:83626620-83626639 None:intergenic 65.0%
GCGCGCGAACTCGCCTGAAC+CGG + chr1.2:83626686-83626705 None:intergenic 70.0%
GGCTCTTCTTCGCCCCCTGC+AGG - chr1.2:83627393-83627412 MS.gene065001:intron 70.0%
! GAGGAGCGTGGGACCGGTTC+AGG - chr1.2:83626670-83626689 MS.gene065001:CDS 70.0%
!! GTAGCGGCCACGGTTCGGTG+AGG + chr1.2:83626625-83626644 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 83626521 83628839 83626521 ID=MS.gene065001
chr1.2 mRNA 83626521 83628839 83626521 ID=MS.gene065001.t1;Parent=MS.gene065001
chr1.2 exon 83628510 83628839 83628510 ID=MS.gene065001.t1.exon1;Parent=MS.gene065001.t1
chr1.2 CDS 83628510 83628839 83628510 ID=cds.MS.gene065001.t1;Parent=MS.gene065001.t1
chr1.2 exon 83627856 83628213 83627856 ID=MS.gene065001.t1.exon2;Parent=MS.gene065001.t1
chr1.2 CDS 83627856 83628213 83627856 ID=cds.MS.gene065001.t1;Parent=MS.gene065001.t1
chr1.2 exon 83626521 83626696 83626521 ID=MS.gene065001.t1.exon3;Parent=MS.gene065001.t1
chr1.2 CDS 83626521 83626696 83626521 ID=cds.MS.gene065001.t1;Parent=MS.gene065001.t1
Gene Sequence

>MS.gene065001

ATGGCTGCCACACCAGCTACTATCCCCGTCCGGTCCGACCGTATCTCTGCCGGTTCATCTTTCCCAAGACCACCACCGGTTCGCAGTCTCGTTAAACCGATCCTCACCGAACCGTGGCCGCTACGTAGAAGGTGGAAGCACTCGTCAACGAGGAGCGTGGGACCGGTTCAGGCGAGTTCGCGCGCAGATGACTCAGCACCGTTCGAGATGTCGGTTGAAAACGCACTGAAGCTGTTAGGTGTATCGGAAGGTGCTTCCTTTGATGATATACTTCGCGCCAAAAACTCCATCGTTGCTTCTTGCAAAGACGACCAAGAAACTATTTCTCAGGTTACACCACTTTACTTGTTGTTGATTTGATTTGATTTGATTTGCAGCTTATTTATTATCAGTTATTTCTTCTATAATGTAAAGTCAAACAACTGTTCCTGTATAAGCTATAATATAAGCTATTTTCATGAGCTGTTATTTATACATGCTTGAGTTTTTTGCCCATGGTCTTGAAATTCATGTACGTACCCCTTGATGTCCTGAATACCTCACAATTTCAGTCTCTAGTTAAGTACTTTTAGTGTTTTATATATTAATTCTATACACCTATTGATGGAATTTCTGTGCATTTTTAGGTCGAGGCTGCATATGACATGTTGCTTATGCAGAGGTTAACTCAGCGGCGAGCAGGAAAAGTAGTGAGCAGCAGTGTTCGTTATGCTGATGTAAAACGTGTTCAGTCCCAGGCTAATAGTTCAATGCCTCAATGGATGCAATCCACCATGAAGAAAGCACCAGTTTCAATTGAGTCACCTTCCACCAGTGATCTTGGTCTACAAGCTGGAGTATATGGTGCACTGATGGGTTTCACCTATTTTAGTGGCTCTTCTTCGCCCCCTGCAGGTTATGCTGGGGCTGATGTTCCGGGACTTATCTTGGCTGGTAGCTTTGGAGCTTCCTTGTACTTCATGACCAAAAAGAATGTTAAGTTAGGTAAGGAGATTTATCATGCACATTGATATTGTAATGCAATGGAATTTTTGTTTCATGAGTGGCAATATTCGTATTGACTTGTATCATGGAATAAAAGTAAGCATTTAGATGGCCATAATGGGTGGGAGAGTAGGATGAAAAAAAAAATGAATGTGATGAGCTTTTCCTTCATGTTTGCCTTTGTTGAAAGAAAAGTTCTAGCAATACTCTATCTTCTATTTGTTAAAATTCATATCAGTTTCACTAAAGTTATGTGGAATCCACATGAATTAGATCCATTAGAACTTTAACCAATAGGAGATAGTGGTTAGAAAATGTTACTAACACCCCTCTTATTGAAACTGTACATTTGAAGACTCATTGCTTTGAAGACACAAAGCAATGAGTGGTATGAGTATGCATAATGAGACCTTGCATCATACAGTGCACCAATTTTGTAGACATTCATAATAAGGTTTTTTTGTATGTTGAATCTTTCACTTACATAGCAACGAGACATGCAACACACCATTCTTTGTCGAAACAATAAGGCCAACCTATTGAATAGACTTCATTTCTTTTGTTATCCCAGACTTTTGATATTAAAAGTTTTGGTATTCTTATACTGCTAATCTGCTGGATATTACTGCTGGTGTCTTTGAAGTGAGGGTTTTATTTACCTAATATGGAGATTGTTTCTGTATAAAATGGTACTGCTGTCAAAAAAGTTGTTTATCTTAACAAATGGTACTTCTAGTTGTGGGTGGTTGAGATCTTTTTCAAAACTTGTAAGACCGTTTACCTCTTTCTTAAACTATTCTCAGTGTAGCTCTACTCTACGTTTAGAAGAGCCAAAATTGACTCCAAAGGTGTATAATTGATTTTGGCATGTTTGGATGTTTTCGGATAGAATTGATTTTGCCTCCAGGATTTATTTTAACTTGAAGATAGAATTTGGGGCTTTTGCCTTTAGAATTGATTTTTAGCTTTGAATTTATTGTATAACCTACTTTCATCTGAATAGCTTTACATTCAACTTACTTTTAGTCAAAATCAATCAATCAAAAAAATTTCTTTGTTACCGCAGAGCCAAATACACTTAATCCTTTTGGATTAGCATAGCATTTTTATTTCTGTTCACATTTTCCTCCTTTTACACTTCCCTTTTCTATTTCTGCAGGGAAGGCTGTTGTTATAACAATAGGAGGGCTCGTAGCCGGTGCTGTTGTAGGGTCAGTTGTAGAGAACTGGCTGCAGGTAGATATTGTCCCATTTCTAGGGATACGCTCCCCAGCTGCTGTTGTTAGCGAAGTCATAATCATCTCTCAGTTCTTGGTTTCGCTATACCTAAGGTAG
Protein sequence

>MS.gene065001.t1

MAATPATIPVRSDRISAGSSFPRPPPVRSLVKPILTEPWPLRRRWKHSSTRSVGPVQASSRADDSAPFEMSVENALKLLGVSEGASFDDILRAKNSIVASCKDDQETISQVEAAYDMLLMQRLTQRRAGKVVSSSVRYADVKRVQSQANSSMPQWMQSTMKKAPVSIESPSTSDLGLQAGVYGALMGFTYFSGSSSPPAGYAGADVPGLILAGSFGASLYFMTKKNVKLGKAVVITIGGLVAGAVVGSVVENWLQVDIVPFLGIRSPAAVVSEVIIISQFLVSLYLR