AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065299

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065299.t1 MTR_7g103300 77.054 353 77 2 1 352 22 371 0.0 512
MS.gene065299.t1 MTR_7g103300 77.054 353 77 2 1 352 112 461 0.0 512
MS.gene065299.t1 MTR_7g103300 77.054 353 77 2 1 352 64 413 0.0 511
MS.gene065299.t1 MTR_7g103300 75.439 342 75 3 1 341 112 445 2.43e-166 472
MS.gene065299.t1 MTR_1g061710 95.951 247 10 0 1 247 113 359 2.52e-151 436
MS.gene065299.t1 MTR_1g061710 99.038 104 1 0 249 352 427 530 7.23e-61 204
MS.gene065299.t1 MTR_7g103300 75.000 284 67 2 1 283 112 392 2.00e-137 396
MS.gene065299.t1 MTR_7g103300 74.400 250 60 2 1 249 112 358 1.31e-116 342
MS.gene065299.t1 MTR_3g109950 49.716 352 164 5 1 351 128 467 2.69e-104 315
MS.gene065299.t1 MTR_3g109950 50.305 328 150 5 1 327 128 443 6.47e-96 293
MS.gene065299.t1 MTR_8g077830 47.278 349 176 2 2 350 125 465 9.80e-96 293
MS.gene065299.t1 MTR_5g019950 49.858 351 164 3 1 351 131 469 9.67e-92 282
MS.gene065299.t1 MTR_8g088080 44.818 357 180 3 2 351 128 474 2.34e-83 261
MS.gene065299.t1 MTR_8g088070 45.070 355 183 4 2 351 122 469 1.18e-82 259
MS.gene065299.t1 MTR_1g026910 42.297 357 188 5 2 350 109 455 1.60e-81 256
MS.gene065299.t1 MTR_3g109950 49.822 281 128 5 1 280 128 396 6.49e-78 245
MS.gene065299.t1 MTR_1g026910 41.617 334 177 5 2 327 109 432 4.03e-72 231
MS.gene065299.t1 MTR_2g010740 30.347 346 202 9 23 346 134 462 9.25e-34 130
MS.gene065299.t1 MTR_5g019970 56.731 104 45 0 248 351 6 109 1.77e-29 109
MS.gene065299.t1 MTR_2g010740 29.688 256 141 9 23 256 134 372 8.69e-21 92.8
MS.gene065299.t1 MTR_3g110012 51.163 86 20 1 218 281 60 145 3.64e-11 61.2
MS.gene065299.t1 MTR_3g109980 51.163 86 20 1 218 281 60 145 3.64e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065299.t1 AT2G36630 73.371 353 90 2 1 352 109 458 3.47e-174 492
MS.gene065299.t1 AT2G25737 50.139 359 157 5 1 351 129 473 1.25e-103 313
MS.gene065299.t1 AT2G25737 47.860 257 112 5 1 249 129 371 1.92e-60 199
MS.gene065299.t1 AT1G11540 28.090 356 231 7 2 346 22 363 2.81e-32 124
MS.gene065299.t1 AT1G11540 28.090 356 231 7 2 346 105 446 1.83e-31 123
MS.gene065299.t1 AT4G21250 29.524 315 186 6 33 330 134 429 6.77e-28 113
MS.gene065299.t1 AT1G61740 25.895 363 234 9 2 346 109 454 6.12e-26 108
MS.gene065299.t1 AT4G21260 29.870 308 170 7 23 330 112 373 1.46e-22 97.8

Find 75 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGGAGGTGGATTTATTT+TGG 0.132041 1.2:-46967342 MS.gene065299:CDS
CCATGAGTTCATGGGATTCT+TGG 0.257074 1.2:-46966326 MS.gene065299:CDS
GCTACTTTGGTTAATTCTAA+TGG 0.257433 1.2:-46968244 MS.gene065299:CDS
ATGCCTCACATATGTATTCT+AGG 0.262467 1.2:+46967444 None:intergenic
TTACCTTGATGATTTGAATA+AGG 0.268202 1.2:+46967900 None:intergenic
GTTGGAGGCTTACTTGGTTC+TGG 0.271593 1.2:-46967359 MS.gene065299:CDS
TTCTCTGTTTCAACCTTAGA+TGG 0.285945 1.2:-46967964 MS.gene065299:CDS
AGAAAATTAGTTGCATTTCT+TGG 0.286067 1.2:-46966574 MS.gene065299:CDS
TTGGGTGCTCTTCGGCTTGC+AGG 0.291877 1.2:-46967749 MS.gene065299:intron
CTAAAATCCTCTTCCATCTA+AGG 0.311247 1.2:+46967951 None:intergenic
GGGCCTCTTCTCCTTGAGAT+AGG 0.312567 1.2:-46967320 MS.gene065299:CDS
AGGACAGTGATAAGCCAAAA+AGG 0.314523 1.2:+46968606 None:intergenic
CCTCATCACAGTGTCTGTTT+TGG 0.316799 1.2:-46966623 MS.gene065299:CDS
CATGAGTTCATGGGATTCTT+GGG 0.327579 1.2:-46966325 MS.gene065299:CDS
CTCATTATCATTCTCTTCAT+AGG 0.331159 1.2:-46968585 MS.gene065299:intron
GCCAAATCATAGTCTAATAT+TGG 0.339930 1.2:+46968693 None:intergenic
GCCAATATTAGACTATGATT+TGG 0.343903 1.2:-46968694 MS.gene065299:CDS
GGCTTACTTGGTTCTGGAGG+TGG 0.346407 1.2:-46967353 MS.gene065299:CDS
GGAATTGTTGGAGGCTTACT+TGG 0.374041 1.2:-46967365 MS.gene065299:CDS
TTTGTGCAGGAACTTCTTTA+AGG 0.376797 1.2:-46968471 MS.gene065299:intron
GAAAATTAGTTGCATTTCTT+GGG 0.377774 1.2:-46966573 MS.gene065299:CDS
AAGAGGTTCCCTATGCCTTA+TGG 0.397338 1.2:-46966745 MS.gene065299:intron
TTTCAGCCAATGCTTATGCT+TGG 0.402032 1.2:-46968663 MS.gene065299:CDS
AACTGCTAAGCAAGCTACTT+TGG 0.413957 1.2:-46968257 MS.gene065299:CDS
TTCAGCCAATGCTTATGCTT+GGG 0.426089 1.2:-46968662 MS.gene065299:CDS
AGGCCTGCAGTGTATGGTAT+TGG 0.427218 1.2:-46967768 MS.gene065299:CDS
TAAAGAAAAGAAGTCAACTA+TGG 0.432207 1.2:-46968073 MS.gene065299:intron
GTCATTGTATTCATCCTCTC+TGG 0.433319 1.2:-46966541 MS.gene065299:CDS
GATACACAACCATGAGTTCA+TGG 0.435806 1.2:-46966335 MS.gene065299:CDS
TGTGCAATGAGTGGTATTGT+AGG 0.441307 1.2:-46967389 MS.gene065299:CDS
GTATGGTATTGGGTGCTCTT+CGG 0.446576 1.2:-46967757 MS.gene065299:CDS
GGGCATCAGCATCATCAGTT+TGG 0.457173 1.2:-46968752 MS.gene065299:CDS
CCTCCTTATTCAAATCATCA+AGG 0.464837 1.2:-46967903 MS.gene065299:intron
GTTTCAACCTTAGATGGAAG+AGG 0.471805 1.2:-46967958 MS.gene065299:CDS
CACAGAATGAGGATGGGCAC+AGG 0.484366 1.2:-46967470 MS.gene065299:CDS
GGCCTGCAGTGTATGGTATT+GGG 0.487342 1.2:-46967767 MS.gene065299:CDS
GGTATTGTAGGTGGAATTGT+TGG 0.495520 1.2:-46967377 MS.gene065299:CDS
TGAAGATTACCATAAGGCAT+AGG 0.509524 1.2:+46966736 None:intergenic
TTTGCCAGTGCTCTCACCAT+GGG 0.516548 1.2:-46966511 MS.gene065299:intron
ATTGTAGGTGGAATTGTTGG+AGG 0.521718 1.2:-46967374 MS.gene065299:CDS
TCTTTAAGGTCATTCTTCAA+AGG 0.527965 1.2:-46968457 MS.gene065299:CDS
CACTCATTGCACAAAATGCA+AGG 0.529460 1.2:+46967399 None:intergenic
CAAAGATCACAGAATGAGGA+TGG 0.531696 1.2:-46967477 MS.gene065299:CDS
TATGTGAGGCATCAATAGAA+TGG 0.532905 1.2:-46967433 MS.gene065299:CDS
ATGTAGAGGCCTGCAGTGTA+TGG 0.533143 1.2:-46967774 MS.gene065299:CDS
TGAGATAGGTGTCATCCCTC+AGG 0.534173 1.2:-46967306 MS.gene065299:intron
TCTAATGGAGAATTTAAAGC+AGG 0.535837 1.2:-46968229 MS.gene065299:intron
CCTTGATGATTTGAATAAGG+AGG 0.539252 1.2:+46967903 None:intergenic
TTGAATTCTACATTCTCAAG+AGG 0.545752 1.2:-46966762 MS.gene065299:CDS
TCAAAGGAATTGAAATGTGG+AGG 0.547426 1.2:-46968441 MS.gene065299:CDS
CCAAGAATCCCATGAACTCA+TGG 0.548829 1.2:+46966326 None:intergenic
ATTACCCATGGTGAGAGCAC+TGG 0.549613 1.2:+46966507 None:intergenic
GGAGGCTTACTTGGTTCTGG+AGG 0.552125 1.2:-46967356 MS.gene065299:CDS
AATTAGTTGCATTTCTTGGG+AGG 0.558277 1.2:-46966570 MS.gene065299:CDS
ATTAGTTGCATTTCTTGGGA+GGG 0.561323 1.2:-46966569 MS.gene065299:CDS
CTATGAAGAGAATGATAATG+AGG 0.573022 1.2:+46968586 None:intergenic
GAAGATTACCATAAGGCATA+GGG 0.576964 1.2:+46966737 None:intergenic
TGTACAAAGATCACAGAATG+AGG 0.585072 1.2:-46967481 MS.gene065299:CDS
CTTATGCTTGGGATAACTGT+TGG 0.586804 1.2:-46968651 MS.gene065299:CDS
TGGTTTGCAGACTGATGTAG+AGG 0.594763 1.2:-46967788 MS.gene065299:intron
GTTATCCCAAGCATAAGCAT+TGG 0.599534 1.2:+46968657 None:intergenic
CAAAGGAATTGAAATGTGGA+GGG 0.605096 1.2:-46968440 MS.gene065299:CDS
TCTTCAAAGGAATTGAAATG+TGG 0.607269 1.2:-46968444 MS.gene065299:CDS
ACACCTATCTCAAGGAGAAG+AGG 0.611353 1.2:+46967317 None:intergenic
ATACACAACCATGAGTTCAT+GGG 0.614070 1.2:-46966334 MS.gene065299:CDS
CACCCAATACCATACACTGC+AGG 0.618863 1.2:+46967765 None:intergenic
ACAGAATGAGGATGGGCACA+GGG 0.625351 1.2:-46967469 MS.gene065299:CDS
AGTTTGGTATAATTTGAGAG+TGG 0.626700 1.2:-46968736 MS.gene065299:CDS
GAGGGATGACACCTATCTCA+AGG 0.628407 1.2:+46967309 None:intergenic
GAGGGTAGAGACTCTTCTCA+AGG 0.635367 1.2:-46968422 MS.gene065299:intron
ACTCATTGCACAAAATGCAA+GGG 0.642562 1.2:+46967400 None:intergenic
AAAGATCACAGAATGAGGAT+GGG 0.663631 1.2:-46967476 MS.gene065299:CDS
GCAATGAGTGGTATTGTAGG+TGG 0.666887 1.2:-46967386 MS.gene065299:CDS
CCAAAACAGACACTGTGATG+AGG 0.668862 1.2:+46966623 None:intergenic
GAGCCTAGAATACATATGTG+AGG 0.685813 1.2:-46967447 MS.gene065299:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTATGTTTATGTTTTTTT+AGG - chr1.2:46968639-46968658 MS.gene065299:CDS 10.0%
!! AAAACAAAAATTAGAGAAAA+AGG + chr1.2:46966772-46966791 None:intergenic 15.0%
!! ATGCTAATTATTTAAATTGT+AGG - chr1.2:46968226-46968245 MS.gene065299:intron 15.0%
!! TAAAATAAACAGTTTATTGT+TGG - chr1.2:46967257-46967276 MS.gene065299:intron 15.0%
!!! ATTTATAGAGTTTTAAGAAA+TGG + chr1.2:46967467-46967486 None:intergenic 15.0%
!!! TTTTATTTTTATTTTTGTGC+AGG - chr1.2:46966581-46966600 MS.gene065299:CDS 15.0%
!!! AATCATTAACTCAAGTTTTT+AGG - chr1.2:46966731-46966750 MS.gene065299:intron 20.0%
!!! ACTGTTTTATTCATTTTTCT+AGG - chr1.2:46966550-46966569 MS.gene065299:CDS 20.0%
!!! ATGTTATACATTTTTGTTTC+AGG - chr1.2:46968677-46968696 MS.gene065299:CDS 20.0%
!!! TCATATTGATAATATTGTAC+TGG + chr1.2:46968399-46968418 None:intergenic 20.0%
!!! TGATCCATTTTCAATTTAAT+TGG - chr1.2:46968373-46968392 MS.gene065299:intron 20.0%
! AAAACTCTATAAATGCAATG+TGG - chr1.2:46967473-46967492 MS.gene065299:CDS 25.0%
! ACTGCAATTCATAATATGTT+TGG + chr1.2:46967363-46967382 None:intergenic 25.0%
! AGAAAATTAGTTGCATTTCT+TGG - chr1.2:46968491-46968510 MS.gene065299:intron 25.0%
! CTATATAAATCAGCAATTTG+AGG + chr1.2:46968596-46968615 None:intergenic 25.0%
! GAAAATTAGTTGCATTTCTT+GGG - chr1.2:46968492-46968511 MS.gene065299:intron 25.0%
! TAAAGAAAAGAAGTCAACTA+TGG - chr1.2:46966992-46967011 MS.gene065299:intron 25.0%
! TATACAAATTTCCACAATAG+TGG - chr1.2:46966859-46966878 MS.gene065299:intron 25.0%
!! TTACCTTGATGATTTGAATA+AGG + chr1.2:46967168-46967187 None:intergenic 25.0%
!!! ATAGTTGACTTCTTTTCTTT+AGG + chr1.2:46966993-46967012 None:intergenic 25.0%
!!! ATTTTTGTTTCAGGTATTGT+TGG - chr1.2:46968686-46968705 MS.gene065299:CDS 25.0%
!!! TAGTTGACTTCTTTTCTTTA+GGG + chr1.2:46966992-46967011 None:intergenic 25.0%
AGATAAGATAACCACTATTG+TGG + chr1.2:46966873-46966892 None:intergenic 30.0%
AGTTTGGTATAATTTGAGAG+TGG - chr1.2:46966329-46966348 MS.gene065299:CDS 30.0%
AGTTTGTGAAGATTACCATA+AGG + chr1.2:46968338-46968357 None:intergenic 30.0%
ATGTTATCAATTGTGGATCA+CGG - chr1.2:46967875-46967894 MS.gene065299:intron 30.0%
CTCATTATCATTCTCTTCAT+AGG - chr1.2:46966480-46966499 MS.gene065299:intron 30.0%
CTGGCCAATTAAATTGAAAA+TGG + chr1.2:46968380-46968399 None:intergenic 30.0%
GCCAAATCATAGTCTAATAT+TGG + chr1.2:46966375-46966394 None:intergenic 30.0%
GCCAATATTAGACTATGATT+TGG - chr1.2:46966371-46966390 MS.gene065299:intron 30.0%
TCTTCAAAGGAATTGAAATG+TGG - chr1.2:46966621-46966640 MS.gene065299:CDS 30.0%
TCTTGACTAGAATTGACTAA+TGG - chr1.2:46966674-46966693 MS.gene065299:intron 30.0%
TTGAATTCTACATTCTCAAG+AGG - chr1.2:46968303-46968322 MS.gene065299:intron 30.0%
TTGACCAATGTTATCAATTG+TGG - chr1.2:46967868-46967887 MS.gene065299:intron 30.0%
TTGGAAACTGAAACAGAAAA+TGG + chr1.2:46967528-46967547 None:intergenic 30.0%
! AATATTGGCACTTCTTTTGT+TGG + chr1.2:46966360-46966379 None:intergenic 30.0%
! ATAAATCAGCAATTTGAGGT+AGG + chr1.2:46968592-46968611 None:intergenic 30.0%
! ATTTTCGCAATTGAAAACTC+TGG + chr1.2:46968013-46968032 None:intergenic 30.0%
! CTATGAAGAGAATGATAATG+AGG + chr1.2:46966482-46966501 None:intergenic 30.0%
! GCTACTTTGGTTAATTCTAA+TGG - chr1.2:46966821-46966840 MS.gene065299:intron 30.0%
! TCAGTTTCCAATTGCATTTT+TGG - chr1.2:46967537-46967556 MS.gene065299:intron 30.0%
! TCTAATGGAGAATTTAAAGC+AGG - chr1.2:46966836-46966855 MS.gene065299:intron 30.0%
! TGATCCACAATTGATAACAT+TGG + chr1.2:46967875-46967894 None:intergenic 30.0%
!! AAACAGAGAATTTGCTGAAA+GGG + chr1.2:46967091-46967110 None:intergenic 30.0%
!! TCTTTAAGGTCATTCTTCAA+AGG - chr1.2:46966608-46966627 MS.gene065299:CDS 30.0%
!! TTCTTTTCTTTAGGGATCAA+TGG + chr1.2:46966984-46967003 None:intergenic 30.0%
AAAGATCACAGAATGAGGAT+GGG - chr1.2:46967589-46967608 MS.gene065299:intron 35.0%
AACAGAACTCTTATAGTGAG+AGG + chr1.2:46967196-46967215 None:intergenic 35.0%
AACATTGGTCAACACAATTG+CGG + chr1.2:46967860-46967879 None:intergenic 35.0%
AATTAGTTGCATTTCTTGGG+AGG - chr1.2:46968495-46968514 MS.gene065299:intron 35.0%
ACAGAAAATGGACATGTGTA+TGG + chr1.2:46967516-46967535 None:intergenic 35.0%
ACAGAACTCTTATAGTGAGA+GGG + chr1.2:46967195-46967214 None:intergenic 35.0%
ACTCATTGCACAAAATGCAA+GGG + chr1.2:46967668-46967687 None:intergenic 35.0%
ATACACAACCATGAGTTCAT+GGG - chr1.2:46968731-46968750 MS.gene065299:CDS 35.0%
ATCACATTGTAGCTATAGTG+TGG + chr1.2:46968069-46968088 None:intergenic 35.0%
ATGCCTCACATATGTATTCT+AGG + chr1.2:46967624-46967643 None:intergenic 35.0%
ATTAGTTGCATTTCTTGGGA+GGG - chr1.2:46968496-46968515 MS.gene065299:intron 35.0%
CAAAGGAATTGAAATGTGGA+GGG - chr1.2:46966625-46966644 MS.gene065299:CDS 35.0%
CCAAACACCAAAAATGCAAT+TGG + chr1.2:46967547-46967566 None:intergenic 35.0%
CCTCCTTATTCAAATCATCA+AGG - chr1.2:46967162-46967181 MS.gene065299:intron 35.0%
CTAAAATCCTCTTCCATCTA+AGG + chr1.2:46967117-46967136 None:intergenic 35.0%
GAAGATTACCATAAGGCATA+GGG + chr1.2:46968331-46968350 None:intergenic 35.0%
GGTAAGCAGAATTGAAAAGA+GGG + chr1.2:46967798-46967817 None:intergenic 35.0%
TCAAAGGAATTGAAATGTGG+AGG - chr1.2:46966624-46966643 MS.gene065299:CDS 35.0%
TCTGGTCAACACAACTAAAA+GGG + chr1.2:46967995-46968014 None:intergenic 35.0%
TGAAGATTACCATAAGGCAT+AGG + chr1.2:46968332-46968351 None:intergenic 35.0%
TGCTTACCAATGTTGTTGAT+CGG - chr1.2:46967810-46967829 MS.gene065299:intron 35.0%
TGGTAAGCAGAATTGAAAAG+AGG + chr1.2:46967799-46967818 None:intergenic 35.0%
TGTACAAAGATCACAGAATG+AGG - chr1.2:46967584-46967603 MS.gene065299:intron 35.0%
TTCTCTGTTTCAACCTTAGA+TGG - chr1.2:46967101-46967120 MS.gene065299:intron 35.0%
TTTGTGCAGGAACTTCTTTA+AGG - chr1.2:46966594-46966613 MS.gene065299:CDS 35.0%
! CCTTGATGATTTGAATAAGG+AGG + chr1.2:46967165-46967184 None:intergenic 35.0%
! TATGTGAGGCATCAATAGAA+TGG - chr1.2:46967632-46967651 MS.gene065299:intron 35.0%
!! GAAACAGAGAATTTGCTGAA+AGG + chr1.2:46967092-46967111 None:intergenic 35.0%
!! GAAGAGGATTTTAGTGCTTT+TGG - chr1.2:46967123-46967142 MS.gene065299:intron 35.0%
!! TCTGGAGGTGGATTTATTTT+GGG - chr1.2:46967724-46967743 MS.gene065299:intron 35.0%
!! TTCTGGAGGTGGATTTATTT+TGG - chr1.2:46967723-46967742 MS.gene065299:intron 35.0%
!!! CCAATTGCATTTTTGGTGTT+TGG - chr1.2:46967544-46967563 MS.gene065299:intron 35.0%
!!! TCAGTGTTGTTTTCCCTTTT+TGG - chr1.2:46966445-46966464 MS.gene065299:intron 35.0%
AACTGCTAAGCAAGCTACTT+TGG - chr1.2:46966808-46966827 MS.gene065299:CDS 40.0%
AATGGTTTATGACCACAGTC+TGG + chr1.2:46967449-46967468 None:intergenic 40.0%
AGGACAGTGATAAGCCAAAA+AGG + chr1.2:46966462-46966481 None:intergenic 40.0%
ATTGTAGGTGGAATTGTTGG+AGG - chr1.2:46967691-46967710 MS.gene065299:intron 40.0%
CAAAGATCACAGAATGAGGA+TGG - chr1.2:46967588-46967607 MS.gene065299:intron 40.0%
CACTCATTGCACAAAATGCA+AGG + chr1.2:46967669-46967688 None:intergenic 40.0%
CATGAGTTCATGGGATTCTT+GGG - chr1.2:46968740-46968759 MS.gene065299:CDS 40.0%
CGCATATCGTTGAACAATAG+CGG + chr1.2:46967937-46967956 None:intergenic 40.0%
CTCTGGTCAACACAACTAAA+AGG + chr1.2:46967996-46968015 None:intergenic 40.0%
CTTATGCTTGGGATAACTGT+TGG - chr1.2:46966414-46966433 MS.gene065299:intron 40.0%
GAGCCTAGAATACATATGTG+AGG - chr1.2:46967618-46967637 MS.gene065299:intron 40.0%
GATACACAACCATGAGTTCA+TGG - chr1.2:46968730-46968749 MS.gene065299:CDS 40.0%
GGAACATATAGTGTACCTGA+GGG + chr1.2:46967777-46967796 None:intergenic 40.0%
GGACAGTGATAAGCCAAAAA+GGG + chr1.2:46966461-46966480 None:intergenic 40.0%
GGTATTGTAGGTGGAATTGT+TGG - chr1.2:46967688-46967707 MS.gene065299:intron 40.0%
GTCATTGTATTCATCCTCTC+TGG - chr1.2:46968524-46968543 MS.gene065299:intron 40.0%
GTTATCCCAAGCATAAGCAT+TGG + chr1.2:46966411-46966430 None:intergenic 40.0%
GTTTCAACCTTAGATGGAAG+AGG - chr1.2:46967107-46967126 MS.gene065299:intron 40.0%
TAGGTGTCCATTATTACCCA+TGG + chr1.2:46968573-46968592 None:intergenic 40.0%
TGGCAAAAATAACACCAGAG+AGG + chr1.2:46968541-46968560 None:intergenic 40.0%
TGTGCAATGAGTGGTATTGT+AGG - chr1.2:46967676-46967695 MS.gene065299:intron 40.0%
TTCAGCCAATGCTTATGCTT+GGG - chr1.2:46966403-46966422 MS.gene065299:intron 40.0%
TTTCAGCCAATGCTTATGCT+TGG - chr1.2:46966402-46966421 MS.gene065299:intron 40.0%
! CTTGCATTTTGTGCAATGAG+TGG - chr1.2:46967667-46967686 MS.gene065299:intron 40.0%
!! TTTTAGTGCTTTTGGCTGTG+TGG - chr1.2:46967131-46967150 MS.gene065299:intron 40.0%
!!! CTGGAGGTGGATTTATTTTG+GGG - chr1.2:46967725-46967744 MS.gene065299:intron 40.0%
AAGAGGTTCCCTATGCCTTA+TGG - chr1.2:46968320-46968339 MS.gene065299:intron 45.0%
ACACCTATCTCAAGGAGAAG+AGG + chr1.2:46967751-46967770 None:intergenic 45.0%
ACAGTCTGGACTGTCACATA+AGG + chr1.2:46967435-46967454 None:intergenic 45.0%
ACTATTGTGTCACAGCGCAA+CGG + chr1.2:46968145-46968164 None:intergenic 45.0%
CCAAAACAGACACTGTGATG+AGG + chr1.2:46968445-46968464 None:intergenic 45.0%
CCAAGAATCCCATGAACTCA+TGG + chr1.2:46968742-46968761 None:intergenic 45.0%
CCATGAGTTCATGGGATTCT+TGG - chr1.2:46968739-46968758 MS.gene065299:CDS 45.0%
CGACTAGAACTGCAAATCTC+AGG + chr1.2:46966947-46966966 None:intergenic 45.0%
GATCGGATCGCAAAAACTAG+CGG - chr1.2:46967827-46967846 MS.gene065299:intron 45.0%
GCAATGAGTGGTATTGTAGG+TGG - chr1.2:46967679-46967698 MS.gene065299:intron 45.0%
GCTCTCACCATGGGTAATAA+TGG - chr1.2:46968563-46968582 MS.gene065299:intron 45.0%
GGAATTGTTGGAGGCTTACT+TGG - chr1.2:46967700-46967719 MS.gene065299:intron 45.0%
GGGAACATATAGTGTACCTG+AGG + chr1.2:46967778-46967797 None:intergenic 45.0%
TGCGATCCGATCAACAACAT+TGG + chr1.2:46967819-46967838 None:intergenic 45.0%
TTATGTGACAGTCCAGACTG+TGG - chr1.2:46967434-46967453 MS.gene065299:CDS 45.0%
! CCTCATCACAGTGTCTGTTT+TGG - chr1.2:46968442-46968461 MS.gene065299:CDS 45.0%
! GTATGGTATTGGGTGCTCTT+CGG - chr1.2:46967308-46967327 MS.gene065299:CDS 45.0%
! TGGTTTGCAGACTGATGTAG+AGG - chr1.2:46967277-46967296 MS.gene065299:intron 45.0%
!!! ATCACAGTGTCTGTTTTGGC+TGG - chr1.2:46968446-46968465 MS.gene065299:CDS 45.0%
ACAGAATGAGGATGGGCACA+GGG - chr1.2:46967596-46967615 MS.gene065299:intron 50.0%
AGGCCTGCAGTGTATGGTAT+TGG - chr1.2:46967297-46967316 MS.gene065299:intron 50.0%
ATGTAGAGGCCTGCAGTGTA+TGG - chr1.2:46967291-46967310 MS.gene065299:intron 50.0%
CACCCAATACCATACACTGC+AGG + chr1.2:46967303-46967322 None:intergenic 50.0%
GAGGGATGACACCTATCTCA+AGG + chr1.2:46967759-46967778 None:intergenic 50.0%
GAGGGTAGAGACTCTTCTCA+AGG - chr1.2:46966643-46966662 MS.gene065299:intron 50.0%
GGCCTGCAGTGTATGGTATT+GGG - chr1.2:46967298-46967317 MS.gene065299:intron 50.0%
GGGCATCAGCATCATCAGTT+TGG - chr1.2:46966313-46966332 MS.gene065299:CDS 50.0%
GTTGGAGGCTTACTTGGTTC+TGG - chr1.2:46967706-46967725 MS.gene065299:intron 50.0%
TTTGCCAGTGCTCTCACCAT+GGG - chr1.2:46968554-46968573 MS.gene065299:intron 50.0%
! ATTACCCATGGTGAGAGCAC+TGG + chr1.2:46968561-46968580 None:intergenic 50.0%
! TGAGATAGGTGTCATCCCTC+AGG - chr1.2:46967759-46967778 MS.gene065299:CDS 50.0%
! TTTTGCCAGTGCTCTCACCA+TGG - chr1.2:46968553-46968572 MS.gene065299:intron 50.0%
!!! GTCTGTTTTGGCTGGCTTCT+GGG - chr1.2:46968454-46968473 MS.gene065299:CDS 50.0%
!!! TCTGTTTTGGCTGGCTTCTG+GGG - chr1.2:46968455-46968474 MS.gene065299:CDS 50.0%
!!! TGTCTGTTTTGGCTGGCTTC+TGG - chr1.2:46968453-46968472 MS.gene065299:CDS 50.0%
CACAGAATGAGGATGGGCAC+AGG - chr1.2:46967595-46967614 MS.gene065299:intron 55.0%
GGGCCTCTTCTCCTTGAGAT+AGG - chr1.2:46967745-46967764 MS.gene065299:intron 55.0%
!! GGAGGCTTACTTGGTTCTGG+AGG - chr1.2:46967709-46967728 MS.gene065299:intron 55.0%
!! GGCTTACTTGGTTCTGGAGG+TGG - chr1.2:46967712-46967731 MS.gene065299:intron 55.0%
! GTGCTCTTCGGCTTGCAGGT+AGG - chr1.2:46967320-46967339 MS.gene065299:CDS 60.0%
! TTGGGTGCTCTTCGGCTTGC+AGG - chr1.2:46967316-46967335 MS.gene065299:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 46966309 46968778 46966309 ID=MS.gene065299
chr1.2 mRNA 46966309 46968778 46966309 ID=MS.gene065299.t1;Parent=MS.gene065299
chr1.2 exon 46968586 46968778 46968586 ID=MS.gene065299.t1.exon1;Parent=MS.gene065299.t1
chr1.2 CDS 46968586 46968778 46968586 ID=cds.MS.gene065299.t1;Parent=MS.gene065299.t1
chr1.2 exon 46968423 46968484 46968423 ID=MS.gene065299.t1.exon2;Parent=MS.gene065299.t1
chr1.2 CDS 46968423 46968484 46968423 ID=cds.MS.gene065299.t1;Parent=MS.gene065299.t1
chr1.2 exon 46968230 46968284 46968230 ID=MS.gene065299.t1.exon3;Parent=MS.gene065299.t1
chr1.2 CDS 46968230 46968284 46968230 ID=cds.MS.gene065299.t1;Parent=MS.gene065299.t1
chr1.2 exon 46968074 46968129 46968074 ID=MS.gene065299.t1.exon4;Parent=MS.gene065299.t1
chr1.2 CDS 46968074 46968129 46968074 ID=cds.MS.gene065299.t1;Parent=MS.gene065299.t1
chr1.2 exon 46967904 46967990 46967904 ID=MS.gene065299.t1.exon5;Parent=MS.gene065299.t1
chr1.2 CDS 46967904 46967990 46967904 ID=cds.MS.gene065299.t1;Parent=MS.gene065299.t1
chr1.2 exon 46967750 46967800 46967750 ID=MS.gene065299.t1.exon6;Parent=MS.gene065299.t1
chr1.2 CDS 46967750 46967800 46967750 ID=cds.MS.gene065299.t1;Parent=MS.gene065299.t1
chr1.2 exon 46967307 46967546 46967307 ID=MS.gene065299.t1.exon7;Parent=MS.gene065299.t1
chr1.2 CDS 46967307 46967546 46967307 ID=cds.MS.gene065299.t1;Parent=MS.gene065299.t1
chr1.2 exon 46966746 46966839 46966746 ID=MS.gene065299.t1.exon8;Parent=MS.gene065299.t1
chr1.2 CDS 46966746 46966839 46966746 ID=cds.MS.gene065299.t1;Parent=MS.gene065299.t1
chr1.2 exon 46966512 46966652 46966512 ID=MS.gene065299.t1.exon9;Parent=MS.gene065299.t1
chr1.2 CDS 46966512 46966652 46966512 ID=cds.MS.gene065299.t1;Parent=MS.gene065299.t1
chr1.2 exon 46966309 46966388 46966309 ID=MS.gene065299.t1.exon10;Parent=MS.gene065299.t1
chr1.2 CDS 46966309 46966388 46966309 ID=cds.MS.gene065299.t1;Parent=MS.gene065299.t1
Gene Sequence

>MS.gene065299

ATGGGGGCATCAGCATCATCAGTTTGGTATAATTTGAGAGTGGCTCATCCAACAAAAGAAGTGCCAATATTAGACTATGATTTGGCTCTTTTGTTTCAGCCAATGCTTATGCTTGGGATAACTGTTGGTGTTACTCTCAGTGTTGTTTTCCCTTTTTGGCTTATCACTGTCCTCATTATCATTCTCTTCATAGGTTTGTATAATCACTAAAATTGTTTTCTTTTTCAAATGAGTCTATTACACTGTTTTATTCATTTTTCTAGGAAACAATATTTTATTTTTATTTTTGTGCAGGAACTTCTTTAAGGTCATTCTTCAAAGGAATTGAAATGTGGAGGGTAGAGACTCTTCTCAAGGTCAACATTTCTTGACTAGAATTGACTAATGGATATATATATGCAGTATGCTTATAATAAGTCATCAATCATTAACTCAAGTTTTTAGGAAAAGTAACTATCTACCTTTTTCTCTAATTTTTGTTTTCTATTTTGCAGAATGAAACTGCTAAGCAAGCTACTTTGGTTAATTCTAATGGAGAATTTAAAGCAGGTATACAAATTTCCACAATAGTGGTTATCTTATCTTTGTATAATTTTAAAGATTTAAAGATAACATCTCATGCTATATTATTGTTTCCTGAGATTTGCAGTTCTAGTCGACACGAAGCTTGAGCCATTGATCCCTAAAGAAAAGAAGTCAACTATGGTATGTTCTATAGCAGATGATAATTGTTTTCATTTTGATGTATTTAAGATGAAATTAATCTTTCTCATCTGAAACCCTTTCAGCAAATTCTCTGTTTCAACCTTAGATGGAAGAGGATTTTAGTGCTTTTGGCTGTGTGGTTTTCTTTCCTCCTTATTCAAATCATCAAGGTAATTTTCCCTCTCACTATAAGAGTTCTGTTAAGTGTTTTTTGTTTTTTTTTTTTTTTTGCTTTTGCTTTTTTAAAATAAACAGTTTATTGTTGGTTTGCAGACTGATGTAGAGGCCTGCAGTGTATGGTATTGGGTGCTCTTCGGCTTGCAGGTAGGATTACAAAATTACATTACCAAACATATTATGAATTGCAGTAATGATTCGCGATCAGATGCAACTTTAGTTATTGTGAATACATCAAAAACCTTATGTGACAGTCCAGACTGTGGTCATAAACCATTTCTTAAAACTCTATAAATGCAATGTGGTTAGACTTTTCTAATTGCCATACACATGTCCATTTTCTGTTTCAGTTTCCAATTGCATTTTTGGTGTTTGGCTTTGAAGCAGTCAAGTTGTACAAAGATCACAGAATGAGGATGGGCACAGGGAGCCTAGAATACATATGTGAGGCATCAATAGAATGGAGTGCTACGTCCCTTGCATTTTGTGCAATGAGTGGTATTGTAGGTGGAATTGTTGGAGGCTTACTTGGTTCTGGAGGTGGATTTATTTTGGGGCCTCTTCTCCTTGAGATAGGTGTCATCCCTCAGGTACACTATATGTTCCCTCTTTTCAATTCTGCTTACCAATGTTGTTGATCGGATCGCAAAAACTAGCGGTCTGTAGCCGCAATTGTGTTGACCAATGTTATCAATTGTGGATCACGGAAAATAGTGATATGTACAAATTATGTTACACTATAACCGCTATTGTTCAACGATATGCGATTTAATATAAAAGTTGTCACACTGCAGCTATGATCCCTTTTAGTTGTGTTGACCAGAGTTTTCAATTGCGAAAATAGTAGTTTGTGCAAATTCTGTTACATGCTCTAGCCACACTATAGCTACAATGTGATAACATTTTTGTACTAAATCGCGTATCACTAAACAACAACTATTTATTCATATTCCGTTGCGCTGTGACACAATAGTGTTGCTACAGTTTCTATTTGACAACACTAGTAGTGACAACTAAGAAACTTGACTTATAAGTATGCTAATTATTTAAATTGTAGGTTGCTAGTGCAACAGCAACATTTGTGATGATGTTTTCATCATCTTTGTCTGTTTTTGAATTCTACATTCTCAAGAGGTTCCCTATGCCTTATGGTAATCTTCACAAACTCTTCAAAGCTTAATTTGATCCATTTTCAATTTAATTGGCCAGTACAATATTATCAATATGAATTTATTTGTTTGCAGCATTATACCTCATCACAGTGTCTGTTTTGGCTGGCTTCTGGGGACAGTTCTTTGTTAGAAAATTAGTTGCATTTCTTGGGAGGGCATCAGTCATTGTATTCATCCTCTCTGGTGTTATTTTTGCCAGTGCTCTCACCATGGGTAATAATGGACACCTACCTCAAATTGCTGATTTATATAGAATGATATTTGCACAAATATTAATATTATGTTTATGTTTTTTTAGGAGTAATATTATATTAATGTTATACATTTTTGTTTCAGGTATTGTTGGCACTGAGAAGAGTATTCAAATGATACACAACCATGAGTTCATGGGATTCTTGGGATTTTGTAGCACTTAA
Protein sequence

>MS.gene065299.t1

MGASASSVWYNLRVAHPTKEVPILDYDLALLFQPMLMLGITVGVTLSVVFPFWLITVLIIILFIGTSLRSFFKGIEMWRVETLLKNETAKQATLVNSNGEFKAVLVDTKLEPLIPKEKKSTMQILCFNLRWKRILVLLAVWFSFLLIQIIKTDVEACSVWYWVLFGLQFPIAFLVFGFEAVKLYKDHRMRMGTGSLEYICEASIEWSATSLAFCAMSGIVGGIVGGLLGSGGGFILGPLLLEIGVIPQVASATATFVMMFSSSLSVFEFYILKRFPMPYALYLITVSVLAGFWGQFFVRKLVAFLGRASVIVFILSGVIFASALTMGIVGTEKSIQMIHNHEFMGFLGFCST