Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065481.t1 | XP_003624429.1 | 98.6 | 72 | 1 | 0 | 115 | 186 | 115 | 186 | 8.80E-31 | 143.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065481.t1 | B9RH17 | 59.4 | 69 | 28 | 0 | 115 | 183 | 119 | 187 | 2.4e-16 | 87.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065481.t1 | G7KW47 | 98.6 | 72 | 1 | 0 | 115 | 186 | 115 | 186 | 6.3e-31 | 143.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene025030 | MS.gene065481 | PPI |
| MS.gene065481 | MS.gene63947 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065481.t1 | MTR_7g083120 | 96.774 | 186 | 6 | 0 | 1 | 186 | 1 | 186 | 1.45e-133 | 371 |
| MS.gene065481.t1 | MTR_7g083120 | 95.726 | 117 | 5 | 0 | 1 | 117 | 1 | 117 | 3.03e-76 | 227 |
| MS.gene065481.t1 | MTR_4g118810 | 29.655 | 145 | 88 | 3 | 33 | 166 | 36 | 177 | 5.29e-16 | 72.8 |
| MS.gene065481.t1 | MTR_0004s0210 | 29.927 | 137 | 88 | 1 | 33 | 169 | 11 | 139 | 1.59e-15 | 70.9 |
| MS.gene065481.t1 | MTR_4g081880 | 32.099 | 162 | 105 | 3 | 11 | 169 | 10 | 169 | 1.85e-14 | 68.6 |
| MS.gene065481.t1 | MTR_7g108130 | 30.496 | 141 | 92 | 3 | 33 | 169 | 32 | 170 | 6.94e-14 | 67.0 |
| MS.gene065481.t1 | MTR_4g118800 | 36.000 | 125 | 79 | 1 | 33 | 157 | 31 | 154 | 9.21e-13 | 63.9 |
| MS.gene065481.t1 | MTR_2g020060 | 27.778 | 162 | 104 | 2 | 33 | 184 | 26 | 184 | 2.74e-12 | 62.8 |
| MS.gene065481.t1 | MTR_7g011090 | 30.435 | 161 | 107 | 3 | 11 | 169 | 11 | 168 | 1.23e-11 | 60.8 |
| MS.gene065481.t1 | MTR_5g076480 | 24.242 | 165 | 118 | 3 | 22 | 186 | 29 | 186 | 3.80e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065481.t1 | AT4G25040 | 36.424 | 151 | 92 | 1 | 31 | 181 | 18 | 164 | 3.48e-21 | 85.9 |
| MS.gene065481.t1 | AT4G03540 | 34.959 | 123 | 72 | 1 | 47 | 169 | 26 | 140 | 6.31e-18 | 77.0 |
| MS.gene065481.t1 | AT4G15630 | 26.984 | 189 | 119 | 4 | 6 | 184 | 10 | 189 | 1.12e-15 | 72.0 |
| MS.gene065481.t1 | AT4G15620 | 30.435 | 161 | 100 | 3 | 34 | 184 | 31 | 189 | 3.41e-15 | 70.5 |
| MS.gene065481.t1 | AT1G03700 | 32.143 | 112 | 68 | 1 | 45 | 156 | 24 | 127 | 3.23e-14 | 67.4 |
| MS.gene065481.t1 | AT4G15610 | 28.105 | 153 | 105 | 2 | 10 | 157 | 9 | 161 | 4.20e-12 | 62.4 |
| MS.gene065481.t1 | AT5G15290 | 30.216 | 139 | 90 | 3 | 33 | 167 | 31 | 166 | 7.69e-12 | 61.6 |
| MS.gene065481.t1 | AT3G06390 | 25.455 | 165 | 108 | 5 | 5 | 157 | 9 | 170 | 4.19e-11 | 59.7 |
Find 29 sgRNAs with CRISPR-Local
Find 87 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGGCCTTCTTTGCCAATT+TGG | 0.186565 | 7.3:+24842757 | MS.gene065481:CDS |
| TTGGCCTTCTTTGCCAATTT+GGG | 0.251825 | 7.3:+24842758 | MS.gene065481:CDS |
| AAAGTTCTGCAAAACCAATT+TGG | 0.258540 | 7.3:+24842715 | MS.gene065481:CDS |
| TAAAATTGTGAGTCCCAAAT+TGG | 0.269455 | 7.3:-24842771 | None:intergenic |
| TTCCATCCTGAAGGAATCTT+TGG | 0.318409 | 7.3:-24841337 | None:intergenic |
| AAGCAAAAGTGAAACCAAAT+TGG | 0.326060 | 7.3:-24842729 | None:intergenic |
| GGATGAGGTTGTTGAAGATT+TGG | 0.339479 | 7.3:-24841361 | None:intergenic |
| ACATCATAGGACTTTCTTCA+TGG | 0.382860 | 7.3:+24841392 | MS.gene065481:CDS |
| CTGTTATGCTCTTCTCTATC+CGG | 0.383937 | 7.3:+24841490 | MS.gene065481:CDS |
| GATGAGGTTGTTGAAGATTT+GGG | 0.392076 | 7.3:-24841360 | None:intergenic |
| TAATTAAGAGGTGAACTTGT+AGG | 0.396788 | 7.3:-24842798 | None:intergenic |
| GTTGGATACGTGGGACAGTA+TGG | 0.412618 | 7.3:+24842650 | MS.gene065481:CDS |
| CTGATGCCAAAGATTCCTTC+AGG | 0.434784 | 7.3:+24841331 | MS.gene065481:CDS |
| TCAAAACATCCTCAGAATCT+TGG | 0.440400 | 7.3:+24841416 | MS.gene065481:CDS |
| GAGAGGAGAAAATAATTAAG+AGG | 0.459921 | 7.3:-24842810 | None:intergenic |
| ATGACGGTGTTGCTGATAGC+TGG | 0.468432 | 7.3:+24842608 | MS.gene065481:CDS |
| ATGGAGAGAAACACGTTGGA+TGG | 0.514689 | 7.3:+24842669 | MS.gene065481:CDS |
| GAGCATAACAGATTGGTTGT+TGG | 0.526003 | 7.3:-24841478 | None:intergenic |
| GAGTCCCAAATTGGCAAAGA+AGG | 0.541836 | 7.3:-24842762 | None:intergenic |
| TAGAGAAGAGCATAACAGAT+TGG | 0.552990 | 7.3:-24841485 | None:intergenic |
| AGATTTGGGTTCCATCCTGA+AGG | 0.555523 | 7.3:-24841346 | None:intergenic |
| AGCCACAGCCGTTGGATACG+TGG | 0.562855 | 7.3:+24842640 | MS.gene065481:CDS |
| TGTTCAGCAGCCACAGCCGT+TGG | 0.589627 | 7.3:+24842632 | MS.gene065481:CDS |
| TCCCACGTATCCAACGGCTG+TGG | 0.593429 | 7.3:-24842642 | None:intergenic |
| GCCACAGCCGTTGGATACGT+GGG | 0.599202 | 7.3:+24842641 | MS.gene065481:CDS |
| TGCCAAAGATTCCTTCAGGA+TGG | 0.603471 | 7.3:+24841335 | MS.gene065481:CDS |
| GAACAATGACCAAGATTCTG+AGG | 0.616784 | 7.3:-24841425 | None:intergenic |
| ATACTGTCCCACGTATCCAA+CGG | 0.624247 | 7.3:-24842648 | None:intergenic |
| CAGTATGGAGAGAAACACGT+TGG | 0.708078 | 7.3:+24842665 | MS.gene065481:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATGACATTATATTTTTTAT+AGG | + | chr7.3:24842246-24842265 | MS.gene065481:intron | 10.0% |
| !!! | ATGACATTATATTTTTTATA+GGG | + | chr7.3:24842247-24842266 | MS.gene065481:intron | 10.0% |
| !! | TCATATGTATAAACAAAAAA+GGG | + | chr7.3:24841584-24841603 | MS.gene065481:intron | 15.0% |
| !! | TTCAATATAATTATACATGT+CGG | - | chr7.3:24842381-24842400 | None:intergenic | 15.0% |
| !! | TTCATATGTATAAACAAAAA+AGG | + | chr7.3:24841583-24841602 | MS.gene065481:intron | 15.0% |
| !! | TTTGTTTATACATATGAAAA+TGG | - | chr7.3:24841581-24841600 | None:intergenic | 15.0% |
| !!! | TGACATTATATTTTTTATAG+GGG | + | chr7.3:24842248-24842267 | MS.gene065481:intron | 15.0% |
| !!! | TGATTTTCTTAAATTATTAG+CGG | + | chr7.3:24842406-24842425 | MS.gene065481:intron | 15.0% |
| !! | AAACAATGACATGTATAATT+TGG | - | chr7.3:24842194-24842213 | None:intergenic | 20.0% |
| !! | ACTTGACTAAAACATTAAAT+GGG | - | chr7.3:24842138-24842157 | None:intergenic | 20.0% |
| !! | ATACATATGAAAATGGAAAA+TGG | - | chr7.3:24841574-24841593 | None:intergenic | 20.0% |
| !! | TACATATGAAAATGGAAAAT+GGG | - | chr7.3:24841573-24841592 | None:intergenic | 20.0% |
| !!! | CTATTATTTCTTCCTATTTT+TGG | + | chr7.3:24841763-24841782 | MS.gene065481:CDS | 20.0% |
| !!! | TCTGTATTTATTTTTGTCTT+TGG | + | chr7.3:24841638-24841657 | MS.gene065481:intron | 20.0% |
| ! | AATAAAAATGAGCTTCAAAC+CGG | - | chr7.3:24841512-24841531 | None:intergenic | 25.0% |
| ! | ATACATGTCATTGTTTATCA+TGG | + | chr7.3:24842198-24842217 | MS.gene065481:intron | 25.0% |
| ! | GACTTGACTAAAACATTAAA+TGG | - | chr7.3:24842139-24842158 | None:intergenic | 25.0% |
| ! | TCTTTCTAATCCACAAAATT+AGG | + | chr7.3:24842516-24842535 | MS.gene065481:intron | 25.0% |
| ! | TTACCAAATCAACCAAAAAT+AGG | - | chr7.3:24841778-24841797 | None:intergenic | 25.0% |
| ! | TTAGAAAGAAAGGTAAATGA+TGG | - | chr7.3:24842505-24842524 | None:intergenic | 25.0% |
| !! | AAATAATAGTCTTTTCGTTG+AGG | - | chr7.3:24841752-24841771 | None:intergenic | 25.0% |
| !! | GGAATATGATTGATAATATG+TGG | + | chr7.3:24842219-24842238 | MS.gene065481:intron | 25.0% |
| !! | TGATAATCAATCTTTTGATG+AGG | + | chr7.3:24841717-24841736 | MS.gene065481:CDS | 25.0% |
| !!! | AAAATTGGTGAATTTTGATC+AGG | + | chr7.3:24842583-24842602 | MS.gene065481:intron | 25.0% |
| !!! | AAGCCAATAACATTTTAACA+TGG | - | chr7.3:24841872-24841891 | None:intergenic | 25.0% |
| !!! | ATTTTGTGGATTAGAAAGAA+AGG | - | chr7.3:24842515-24842534 | None:intergenic | 25.0% |
| !!! | CATGTTTGTTATGAGAAAAT+TGG | + | chr7.3:24842568-24842587 | MS.gene065481:intron | 25.0% |
| !!! | TTACTAGTGTCAATTTTAGT+GGG | + | chr7.3:24842051-24842070 | MS.gene065481:intron | 25.0% |
| !!! | TTCACTTTTGCTTTCATATT+TGG | + | chr7.3:24842739-24842758 | MS.gene065481:CDS | 25.0% |
| AAAGTTCTGCAAAACCAATT+TGG | + | chr7.3:24842715-24842734 | MS.gene065481:CDS | 30.0% | |
| AAGCAAAAGTGAAACCAAAT+TGG | - | chr7.3:24842732-24842751 | None:intergenic | 30.0% | |
| GGTCCATGTTAAAATGTTAT+TGG | + | chr7.3:24841866-24841885 | MS.gene065481:intron | 30.0% | |
| TAAAATTGTGAGTCCCAAAT+TGG | - | chr7.3:24842774-24842793 | None:intergenic | 30.0% | |
| TCTTAAATTATTAGCGGTGT+CGG | + | chr7.3:24842412-24842431 | MS.gene065481:intron | 30.0% | |
| ! | CTTACTAGTGTCAATTTTAG+TGG | + | chr7.3:24842050-24842069 | MS.gene065481:intron | 30.0% |
| !!! | AATAGTTTTGAGTTAGACCT+TGG | + | chr7.3:24841995-24842014 | MS.gene065481:intron | 30.0% |
| !!! | AGATTTTTTGTTGCTGCAAA+TGG | + | chr7.3:24841665-24841684 | MS.gene065481:intron | 30.0% |
| !!! | CTTCCTATTTTTGGTTGATT+TGG | + | chr7.3:24841772-24841791 | MS.gene065481:CDS | 30.0% |
| ACATCATAGGACTTTCTTCA+TGG | + | chr7.3:24841392-24841411 | MS.gene065481:CDS | 35.0% | |
| CATCCTCACAAAAACATCAT+AGG | + | chr7.3:24841379-24841398 | MS.gene065481:CDS | 35.0% | |
| GATGAGGTTGTTGAAGATTT+GGG | - | chr7.3:24841363-24841382 | None:intergenic | 35.0% | |
| TATCCGTATTCATGCTTCAT+AGG | + | chr7.3:24842467-24842486 | MS.gene065481:intron | 35.0% | |
| TCAAAACATCCTCAGAATCT+TGG | + | chr7.3:24841416-24841435 | MS.gene065481:CDS | 35.0% | |
| TGACCTATGAAGCATGAATA+CGG | - | chr7.3:24842473-24842492 | None:intergenic | 35.0% | |
| ! | CTTATTCGCTCCTAATTTTG+TGG | - | chr7.3:24842529-24842548 | None:intergenic | 35.0% |
| ! | TAGAGAAGAGCATAACAGAT+TGG | - | chr7.3:24841488-24841507 | None:intergenic | 35.0% |
| ! | TTCACAGATGTCTTTTACTC+TGG | + | chr7.3:24842350-24842369 | MS.gene065481:intron | 35.0% |
| ! | TTGGCTTAGACATGTTTTGT+CGG | + | chr7.3:24841885-24841904 | MS.gene065481:intron | 35.0% |
| ! | TTTTGCAGAACTTTTGCACA+TGG | - | chr7.3:24842709-24842728 | None:intergenic | 35.0% |
| !! | AGTCCTATGATGTTTTTGTG+AGG | - | chr7.3:24841385-24841404 | None:intergenic | 35.0% |
| !! | ATGATGTTTTTGTGAGGATG+AGG | - | chr7.3:24841379-24841398 | None:intergenic | 35.0% |
| !! | GAATTTTGATCAGGTGATGA+CGG | + | chr7.3:24842592-24842611 | MS.gene065481:intron | 35.0% |
| !! | TGAAAATGGAAAATGGGTTG+TGG | - | chr7.3:24841567-24841586 | None:intergenic | 35.0% |
| !!! | GTCAATTTTAGTGGGTTAGT+CGG | + | chr7.3:24842059-24842078 | MS.gene065481:intron | 35.0% |
| ATTGAAGTCGAGACTTTGAG+AGG | - | chr7.3:24842029-24842048 | None:intergenic | 40.0% | |
| CTGTTATGCTCTTCTCTATC+CGG | + | chr7.3:24841490-24841509 | MS.gene065481:CDS | 40.0% | |
| GAACAATGACCAAGATTCTG+AGG | - | chr7.3:24841428-24841447 | None:intergenic | 40.0% | |
| GACAGTAGCTATGTTATGTC+TGG | + | chr7.3:24842438-24842457 | MS.gene065481:intron | 40.0% | |
| GAGTTGTAACCTACTTAGGT+TGG | + | chr7.3:24841964-24841983 | MS.gene065481:intron | 40.0% | |
| GGATGAGGTTGTTGAAGATT+TGG | - | chr7.3:24841364-24841383 | None:intergenic | 40.0% | |
| TCAACCAAACCAACCTAAGT+AGG | - | chr7.3:24841976-24841995 | None:intergenic | 40.0% | |
| TGGTTAATTACCTCAGCACT+AGG | - | chr7.3:24842174-24842193 | None:intergenic | 40.0% | |
| TGTCTGAAAACAGAAATCGC+AGG | + | chr7.3:24841845-24841864 | MS.gene065481:intron | 40.0% | |
| TTCCATCCTGAAGGAATCTT+TGG | - | chr7.3:24841340-24841359 | None:intergenic | 40.0% | |
| ! | GAGCATAACAGATTGGTTGT+TGG | - | chr7.3:24841481-24841500 | None:intergenic | 40.0% |
| ! | TTGGCCTTCTTTGCCAATTT+GGG | + | chr7.3:24842758-24842777 | MS.gene065481:CDS | 40.0% |
| ! | TTTGAGAGGAGTATACTCCA+AGG | - | chr7.3:24842015-24842034 | None:intergenic | 40.0% |
| ! | TTTGGCCTTCTTTGCCAATT+TGG | + | chr7.3:24842757-24842776 | MS.gene065481:CDS | 40.0% |
| ! | TTTTGTTGCTGCAAATGGTG+TGG | + | chr7.3:24841670-24841689 | MS.gene065481:CDS | 40.0% |
| !! | GTAACCTACTTAGGTTGGTT+TGG | + | chr7.3:24841969-24841988 | MS.gene065481:intron | 40.0% |
| ATACTGTCCCACGTATCCAA+CGG | - | chr7.3:24842651-24842670 | None:intergenic | 45.0% | |
| ATGGAGAGAAACACGTTGGA+TGG | + | chr7.3:24842669-24842688 | MS.gene065481:CDS | 45.0% | |
| CAGTATGGAGAGAAACACGT+TGG | + | chr7.3:24842665-24842684 | MS.gene065481:CDS | 45.0% | |
| CGCAGAGTTGTAACCTACTT+AGG | + | chr7.3:24841960-24841979 | MS.gene065481:intron | 45.0% | |
| CTGATGCCAAAGATTCCTTC+AGG | + | chr7.3:24841331-24841350 | MS.gene065481:CDS | 45.0% | |
| GAGTCCCAAATTGGCAAAGA+AGG | - | chr7.3:24842765-24842784 | None:intergenic | 45.0% | |
| TGCCAAAGATTCCTTCAGGA+TGG | + | chr7.3:24841335-24841354 | MS.gene065481:CDS | 45.0% | |
| TTACCTCAGCACTAGGCAAA+CGG | - | chr7.3:24842167-24842186 | None:intergenic | 45.0% | |
| ! | AGATTTGGGTTCCATCCTGA+AGG | - | chr7.3:24841349-24841368 | None:intergenic | 45.0% |
| !!! | ATATTTTTTTTTATGTAAAT+CGG | + | chr7.3:24841927-24841946 | MS.gene065481:intron | 5.0% |
| GTTGGATACGTGGGACAGTA+TGG | + | chr7.3:24842650-24842669 | MS.gene065481:CDS | 50.0% | |
| !! | ATGACGGTGTTGCTGATAGC+TGG | + | chr7.3:24842608-24842627 | MS.gene065481:CDS | 50.0% |
| TCTCCGTTTGCCTAGTGCTG+AGG | + | chr7.3:24842161-24842180 | MS.gene065481:intron | 55.0% | |
| AGCCACAGCCGTTGGATACG+TGG | + | chr7.3:24842640-24842659 | MS.gene065481:CDS | 60.0% | |
| GCCACAGCCGTTGGATACGT+GGG | + | chr7.3:24842641-24842660 | MS.gene065481:CDS | 60.0% | |
| TCCCACGTATCCAACGGCTG+TGG | - | chr7.3:24842645-24842664 | None:intergenic | 60.0% | |
| TGTTCAGCAGCCACAGCCGT+TGG | + | chr7.3:24842632-24842651 | MS.gene065481:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 24841327 | 24842817 | 24841327 | ID=MS.gene065481 |
| chr7.3 | mRNA | 24841327 | 24842817 | 24841327 | ID=MS.gene065481.t1;Parent=MS.gene065481 |
| chr7.3 | exon | 24841327 | 24841547 | 24841327 | ID=MS.gene065481.t1.exon1;Parent=MS.gene065481.t1 |
| chr7.3 | CDS | 24841327 | 24841547 | 24841327 | ID=cds.MS.gene065481.t1;Parent=MS.gene065481.t1 |
| chr7.3 | exon | 24841667 | 24841793 | 24841667 | ID=MS.gene065481.t1.exon2;Parent=MS.gene065481.t1 |
| chr7.3 | CDS | 24841667 | 24841793 | 24841667 | ID=cds.MS.gene065481.t1;Parent=MS.gene065481.t1 |
| chr7.3 | exon | 24842605 | 24842817 | 24842605 | ID=MS.gene065481.t1.exon3;Parent=MS.gene065481.t1 |
| chr7.3 | CDS | 24842605 | 24842817 | 24842605 | ID=cds.MS.gene065481.t1;Parent=MS.gene065481.t1 |
| Gene Sequence |
| Protein sequence |
