Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065648.t1 | XP_003592552.2 | 99.4 | 179 | 1 | 0 | 1 | 179 | 1 | 179 | 9.40E-93 | 349.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065648.t1 | Q9C998 | 86.2 | 145 | 20 | 0 | 34 | 178 | 27 | 171 | 3.8e-66 | 252.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065648.t1 | G7IAT2 | 99.4 | 179 | 1 | 0 | 1 | 179 | 1 | 179 | 6.8e-93 | 349.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060330 | MS.gene065648 | 0.802959 | 4.38E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065648.t1 | MTR_1g108440 | 98.889 | 180 | 2 | 0 | 1 | 180 | 1 | 180 | 1.83e-128 | 363 |
| MS.gene065648.t1 | MTR_7g114900 | 67.456 | 169 | 45 | 1 | 31 | 199 | 44 | 202 | 5.45e-75 | 228 |
| MS.gene065648.t1 | MTR_2g032540 | 30.457 | 197 | 108 | 4 | 9 | 179 | 1 | 194 | 1.16e-19 | 85.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065648.t1 | AT2G01290 | 68.718 | 195 | 49 | 4 | 6 | 193 | 7 | 196 | 1.96e-87 | 259 |
| MS.gene065648.t1 | AT1G71100 | 85.034 | 147 | 22 | 0 | 34 | 180 | 27 | 173 | 3.73e-87 | 258 |
| MS.gene065648.t1 | AT3G04790 | 63.934 | 183 | 49 | 2 | 24 | 199 | 26 | 198 | 9.44e-72 | 219 |
| MS.gene065648.t1 | AT5G44520 | 33.103 | 145 | 86 | 4 | 43 | 179 | 43 | 184 | 2.58e-12 | 65.1 |
| MS.gene065648.t1 | AT5G44520 | 33.103 | 145 | 86 | 4 | 43 | 179 | 73 | 214 | 3.40e-12 | 64.7 |
Find 0 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATCAAAACTTGTTAACTATT+TGG | - | 10693:8433-8452 | MS.gene065648:CDS | 20.0% |
| !!! | TCAAAACTTGTTAACTATTT+GGG | - | 10693:8434-8453 | MS.gene065648:CDS | 20.0% |
| ! | AACCTTATGTTACTGATAAT+GGG | - | 10693:8584-8603 | MS.gene065648:CDS | 25.0% |
| AGTTGATCCTTATCTGAATT+TGG | - | 10693:8331-8350 | MS.gene065648:CDS | 30.0% | |
| GAACCTTATGTTACTGATAA+TGG | - | 10693:8583-8602 | MS.gene065648:CDS | 30.0% | |
| GGAAAACTCAAGAACATAAT+CGG | - | 10693:8200-8219 | MS.gene065648:CDS | 30.0% | |
| TTCCCATTATCAGTAACATA+AGG | + | 10693:8589-8608 | MS.gene065648:intergenic | 30.0% | |
| ! | GATTTTCTTCAAATCGTCTT+GGG | + | 10693:8072-8091 | MS.gene065648:intergenic | 30.0% |
| !! | AAACTTGTTAACTATTTGGG+TGG | - | 10693:8437-8456 | MS.gene065648:CDS | 30.0% |
| !! | TTCACTTTTGAGAGAGAAAA+TGG | - | 10693:8370-8389 | MS.gene065648:CDS | 30.0% |
| !! | TTGAAGTTATTCGCTTTTGT+TGG | - | 10693:8480-8499 | MS.gene065648:CDS | 30.0% |
| CAAAAGCGAATAACTTCAAC+AGG | + | 10693:8479-8498 | MS.gene065648:intergenic | 35.0% | |
| CAATAGCCAGATCAATAACA+GGG | + | 10693:8298-8317 | MS.gene065648:intergenic | 35.0% | |
| CCTTATCTGAATTTGGTCAA+AGG | - | 10693:8338-8357 | MS.gene065648:CDS | 35.0% | |
| CCTTTGACCAAATTCAGATA+AGG | + | 10693:8341-8360 | MS.gene065648:intergenic | 35.0% | |
| CTTAGAACTTTCGGTGAAAA+GGG | - | 10693:8559-8578 | MS.gene065648:CDS | 35.0% | |
| GTTATTGATCTGGCTATTGA+TGG | - | 10693:8299-8318 | MS.gene065648:CDS | 35.0% | |
| GTTGCAAAGCTTAGAACTTT+CGG | - | 10693:8550-8569 | MS.gene065648:CDS | 35.0% | |
| TCAATAGCCAGATCAATAAC+AGG | + | 10693:8299-8318 | MS.gene065648:intergenic | 35.0% | |
| ! | CGATTTTCTTCAAATCGTCT+TGG | + | 10693:8073-8092 | MS.gene065648:intergenic | 35.0% |
| !! | ACTTTTGAGAGAGAAAATGG+TGG | - | 10693:8373-8392 | MS.gene065648:CDS | 35.0% |
| AACAGGGTGAGAATCTAGAT+CGG | + | 10693:8282-8301 | MS.gene065648:intergenic | 40.0% | |
| CAGAATCTGTTTGAGGAATC+TGG | - | 10693:8523-8542 | MS.gene065648:CDS | 40.0% | |
| GCTTAGAACTTTCGGTGAAA+AGG | - | 10693:8558-8577 | MS.gene065648:CDS | 40.0% | |
| GTTAACTATTTGGGTGGTAG+TGG | - | 10693:8443-8462 | MS.gene065648:CDS | 40.0% | |
| TAGGCTTCAGAATCTGTTTG+AGG | - | 10693:8516-8535 | MS.gene065648:CDS | 40.0% | |
| TCTTCAAATCGTCTTGGGAT+AGG | + | 10693:8067-8086 | MS.gene065648:intergenic | 40.0% | |
| TTCTCACCCTGTTATTGATC+TGG | - | 10693:8289-8308 | MS.gene065648:CDS | 40.0% | |
| ! | CTTTTGTTGGAATTCACTGC+TGG | - | 10693:8493-8512 | MS.gene065648:CDS | 40.0% |
| ! | TCATGGGTTATGGTTGAAGT+GGG | + | 10693:8230-8249 | MS.gene065648:intergenic | 40.0% |
| ! | TGTTCTTGAGTTTTCCCTGA+CGG | + | 10693:8196-8215 | MS.gene065648:intergenic | 40.0% |
| ! | TTCATGGGTTATGGTTGAAG+TGG | + | 10693:8231-8250 | MS.gene065648:intergenic | 40.0% |
| ! | TTGAGAGAGAAAATGGTGGA+AGG | - | 10693:8377-8396 | MS.gene065648:CDS | 40.0% |
| ! | TTTTGCGGAGGAGATGAAAT+GGG | + | 10693:7985-8004 | MS.gene065648:intergenic | 40.0% |
| AATTTGGTCAAAGGTCGTGG+TGG | - | 10693:8347-8366 | MS.gene065648:CDS | 45.0% | |
| ATCGTCTTGGGATAGGATGA+CGG | + | 10693:8060-8079 | MS.gene065648:intergenic | 45.0% | |
| CTGAATTTGGTCAAAGGTCG+TGG | - | 10693:8344-8363 | MS.gene065648:CDS | 45.0% | |
| TGAGAATCTAGATCGGAGAG+AGG | + | 10693:8275-8294 | MS.gene065648:intergenic | 45.0% | |
| TGTTGGAATTCACTGCTGGT+AGG | - | 10693:8497-8516 | MS.gene065648:CDS | 45.0% | |
| TTTGCGGAGGAGATGAAATG+GGG | + | 10693:7984-8003 | MS.gene065648:intergenic | 45.0% | |
| ! | AAGCGACACAGCTTGTTCAT+GGG | + | 10693:8246-8265 | MS.gene065648:intergenic | 45.0% |
| ! | CACAGCTTGTTCATGGGTTA+TGG | + | 10693:8240-8259 | MS.gene065648:intergenic | 45.0% |
| ! | CTTTTGCGGAGGAGATGAAA+TGG | + | 10693:7986-8005 | MS.gene065648:intergenic | 45.0% |
| !! | ATGAACAAGCTGTGTCGCTT+GGG | - | 10693:8246-8265 | MS.gene065648:CDS | 45.0% |
| AAGATGGGGAGGAAGGGATT+AGG | + | 10693:8028-8047 | MS.gene065648:intergenic | 50.0% | |
| AAGCTCTCCAATTCTGGCGA+CGG | + | 10693:8171-8190 | MS.gene065648:intergenic | 50.0% | |
| AATTGGAGAGCTTCTCCGTC+AGG | - | 10693:8178-8197 | MS.gene065648:CDS | 50.0% | |
| ACCGGATTCGACGTATTCGA+CGG | + | 10693:8108-8127 | MS.gene065648:intergenic | 50.0% | |
| ACGGAGAAGCTCTCCAATTC+TGG | + | 10693:8177-8196 | MS.gene065648:intergenic | 50.0% | |
| ATTGGAGAGCTTCTCCGTCA+GGG | - | 10693:8179-8198 | MS.gene065648:CDS | 50.0% | |
| CGAATACGTCGAATCCGGTA+TGG | - | 10693:8109-8128 | MS.gene065648:CDS | 50.0% | |
| GTCGAATCCGGTATGGTTCT+AGG | - | 10693:8116-8135 | MS.gene065648:CDS | 50.0% | |
| TTGCGGAGGAGATGAAATGG+GGG | + | 10693:7983-8002 | MS.gene065648:intergenic | 50.0% | |
| ! | CAAGCGACACAGCTTGTTCA+TGG | + | 10693:8247-8266 | MS.gene065648:intergenic | 50.0% |
| ! | CATGAACAAGCTGTGTCGCT+TGG | - | 10693:8245-8264 | MS.gene065648:CDS | 50.0% |
| ! | GACGTATTCGACGGCTTTGT+AGG | + | 10693:8099-8118 | MS.gene065648:intergenic | 50.0% |
| ! | GTATTCGACGGCTTTGTAGG+CGG | + | 10693:8096-8115 | MS.gene065648:intergenic | 50.0% |
| !! | TTGGGATAGGATGACGGAGA+CGG | + | 10693:8054-8073 | MS.gene065648:intergenic | 50.0% |
| AAACACGCCGTCGCCAGAAT+TGG | - | 10693:8161-8180 | MS.gene065648:CDS | 55.0% | |
| AATTCTGGCGACGGCGTGTT+TGG | + | 10693:8162-8181 | MS.gene065648:intergenic | 55.0% | |
| GAGGAGATGAAATGGGGGCA+AGG | + | 10693:7978-7997 | MS.gene065648:intergenic | 55.0% | |
| GCCGTCGAATACGTCGAATC+CGG | - | 10693:8104-8123 | MS.gene065648:CDS | 55.0% | |
| GGAGGAAGGGATTAGGAGAC+CGG | + | 10693:8021-8040 | MS.gene065648:intergenic | 55.0% | |
| TCCGAGGCCTAGAACCATAC+CGG | + | 10693:8126-8145 | MS.gene065648:intergenic | 55.0% | |
| TGACGGAGACGGAGGAAGAT+GGG | + | 10693:8043-8062 | MS.gene065648:intergenic | 55.0% | |
| ! | ATGACGGAGACGGAGGAAGA+TGG | + | 10693:8044-8063 | MS.gene065648:intergenic | 55.0% |
| ! | GATTAGGAGACCGGCTTCCA+TGG | + | 10693:8012-8031 | MS.gene065648:intergenic | 55.0% |
| !! | ATGGTTCTAGGCCTCGGAAC+CGG | - | 10693:8128-8147 | MS.gene065648:CDS | 55.0% |
| !! | TCCGGTATGGTTCTAGGCCT+CGG | - | 10693:8122-8141 | MS.gene065648:CDS | 55.0% |
| ACGGAGGAAGATGGGGAGGA+AGG | + | 10693:8035-8054 | MS.gene065648:intergenic | 60.0% | |
| CGGAGGAAGATGGGGAGGAA+GGG | + | 10693:8034-8053 | MS.gene065648:intergenic | 60.0% | |
| GACGGAGACGGAGGAAGATG+GGG | + | 10693:8042-8061 | MS.gene065648:intergenic | 60.0% | |
| GCAAAAGCCGCCATGGAAGC+CGG | - | 10693:7999-8018 | MS.gene065648:CDS | 60.0% | |
| ! | GGCTTCCATGGCGGCTTTTG+CGG | + | 10693:8000-8019 | MS.gene065648:intergenic | 60.0% |
| ! | TAGGAGACCGGCTTCCATGG+CGG | + | 10693:8009-8028 | MS.gene065648:intergenic | 60.0% |
| ! | TTCCATGGCGGCTTTTGCGG+AGG | + | 10693:7997-8016 | MS.gene065648:intergenic | 60.0% |
| !! | GGATAGGATGACGGAGACGG+AGG | + | 10693:8051-8070 | MS.gene065648:intergenic | 60.0% |
| CTCCTCCGCAAAAGCCGCCA+TGG | - | 10693:7992-8011 | MS.gene065648:CDS | 65.0% | |
| GGAGACGGAGGAAGATGGGG+AGG | + | 10693:8039-8058 | MS.gene065648:intergenic | 65.0% | |
| GGCGTGTTTGGCGGTTGAGC+CGG | + | 10693:8150-8169 | MS.gene065648:intergenic | 65.0% | |
| TCTGGCGACGGCGTGTTTGG+CGG | + | 10693:8159-8178 | MS.gene065648:intergenic | 65.0% | |
| ! | TGGCGGTTGAGCCGGTTCCG+AGG | + | 10693:8142-8161 | MS.gene065648:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 10693 | gene | 7966 | 8616 | 7966 | ID=MS.gene065648 |
| 10693 | mRNA | 7966 | 8616 | 7966 | ID=MS.gene065648.t1;Parent=MS.gene065648 |
| 10693 | exon | 7966 | 8616 | 7966 | ID=MS.gene065648.t1.exon1;Parent=MS.gene065648.t1 |
| 10693 | CDS | 7966 | 8616 | 7966 | ID=cds.MS.gene065648.t1;Parent=MS.gene065648.t1 |
| Gene Sequence |
| Protein sequence |
