AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065760

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065760.t1 MTR_6g083210 93.578 109 7 0 1 109 170 278 5.69e-74 223
MS.gene065760.t1 MTR_6g083290 93.578 109 7 0 1 109 182 290 1.07e-73 223
MS.gene065760.t1 MTR_6g083270 93.578 109 7 0 1 109 325 433 2.17e-72 224
MS.gene065760.t1 MTR_6g083300 88.073 109 13 0 1 109 170 278 1.37e-68 209
MS.gene065760.t1 MTR_6g082950 87.156 109 14 0 1 109 290 398 2.20e-67 210
MS.gene065760.t1 MTR_6g082870 86.239 109 15 0 1 109 527 635 2.28e-64 207
MS.gene065760.t1 MTR_6g083760 83.486 109 18 0 1 109 490 598 2.37e-63 204
MS.gene065760.t1 MTR_6g082860 71.028 107 30 1 1 107 226 331 4.87e-50 163
MS.gene065760.t1 MTR_6g083780 71.963 107 29 1 1 107 519 624 2.17e-49 167
MS.gene065760.t1 MTR_6g082830 70.093 107 31 1 1 107 170 275 3.48e-49 159
MS.gene065760.t1 MTR_6g083740 74.468 94 24 0 14 107 122 215 2.68e-46 150
MS.gene065760.t1 MTR_8g104520 66.364 110 34 3 1 107 510 619 3.43e-44 153
MS.gene065760.t1 MTR_1g110140 57.547 106 45 0 2 107 223 328 1.15e-40 139
MS.gene065760.t1 MTR_1g110260 57.658 111 47 0 2 112 401 511 2.90e-39 139
MS.gene065760.t1 MTR_1g110220 56.604 106 46 0 2 107 421 526 3.04e-39 139
MS.gene065760.t1 MTR_7g085860 57.944 107 44 1 1 107 331 436 3.87e-38 134
MS.gene065760.t1 MTR_7g082280 56.481 108 46 1 1 107 593 700 5.49e-37 134
MS.gene065760.t1 MTR_7g082470 56.481 108 46 1 1 107 500 607 5.58e-37 133
MS.gene065760.t1 MTR_7g082510 54.206 107 49 0 1 107 512 618 8.85e-37 132
MS.gene065760.t1 MTR_7g082360 56.481 108 43 3 1 107 204 308 2.24e-36 128
MS.gene065760.t1 MTR_7g082300 56.075 107 47 0 1 107 716 822 3.31e-36 131
MS.gene065760.t1 MTR_7g082430 54.206 107 49 0 1 107 481 587 5.65e-36 130
MS.gene065760.t1 MTR_7g082490 53.271 107 50 0 1 107 277 383 7.69e-36 127
MS.gene065760.t1 MTR_4g044393 52.336 107 51 0 1 107 700 806 9.80e-36 130
MS.gene065760.t1 MTR_1g115485 54.206 107 49 0 1 107 527 633 1.38e-35 129
MS.gene065760.t1 MTR_7g082380 53.704 108 49 1 1 107 227 334 2.00e-35 125
MS.gene065760.t1 MTR_7g082400 53.704 108 49 1 1 107 250 357 1.06e-34 124
MS.gene065760.t1 MTR_7g082460 54.206 107 49 0 1 107 516 622 2.85e-34 125
MS.gene065760.t1 MTR_0394s0020 49.533 107 54 0 1 107 240 346 1.15e-33 121
MS.gene065760.t1 MTR_1g110230 50.909 110 51 1 1 107 463 572 3.68e-33 122
MS.gene065760.t1 MTR_1g027690 48.673 113 56 2 1 113 454 564 5.88e-33 122
MS.gene065760.t1 MTR_7g082110 50.467 107 53 0 1 107 702 808 6.05e-33 122
MS.gene065760.t1 MTR_1g027460 47.222 108 54 2 1 107 195 300 2.30e-32 117
MS.gene065760.t1 MTR_1g028290 49.558 113 56 1 1 113 455 566 2.85e-32 120
MS.gene065760.t1 MTR_1g031430 46.729 107 56 1 1 107 48 153 6.45e-32 112
MS.gene065760.t1 MTR_4g073230 50.000 114 54 2 1 113 465 576 8.75e-32 119
MS.gene065760.t1 MTR_1g027250 45.794 107 57 1 1 107 168 273 2.10e-31 114
MS.gene065760.t1 MTR_1g027160 45.794 107 57 1 1 107 168 273 2.76e-31 114
MS.gene065760.t1 MTR_1g027320 43.307 127 66 4 1 125 168 290 3.21e-31 114
MS.gene065760.t1 MTR_1g031280 49.533 107 53 1 1 107 512 617 5.16e-31 116
MS.gene065760.t1 MTR_1g026990 41.985 131 72 3 1 130 168 295 1.12e-30 112
MS.gene065760.t1 MTR_1g031530 45.794 107 57 1 1 107 196 301 1.82e-30 112
MS.gene065760.t1 MTR_1g028130 43.307 127 66 3 1 127 456 576 3.90e-30 114
MS.gene065760.t1 MTR_1g028080 43.307 127 66 3 1 127 456 576 4.74e-30 114
MS.gene065760.t1 MTR_1g027990 44.643 112 62 0 1 112 561 672 4.99e-30 114
MS.gene065760.t1 MTR_1g027540 44.860 107 58 1 1 107 270 375 7.41e-30 112
MS.gene065760.t1 MTR_1g031580 48.276 116 57 2 1 116 467 579 1.59e-29 112
MS.gene065760.t1 MTR_1g028280 46.729 107 56 1 1 107 539 644 1.78e-29 112
MS.gene065760.t1 MTR_1g028280 46.729 107 56 1 1 107 500 605 1.85e-29 112
MS.gene065760.t1 MTR_1g110270 51.485 101 43 1 2 102 489 583 2.17e-29 112
MS.gene065760.t1 MTR_1g031540 50.926 108 50 2 1 107 454 559 2.21e-29 112
MS.gene065760.t1 MTR_1g031540 50.926 108 50 2 1 107 162 267 2.51e-29 112
MS.gene065760.t1 MTR_1g027680 44.643 112 62 0 1 112 558 669 2.96e-29 111
MS.gene065760.t1 MTR_1g031320 49.533 107 53 1 1 107 471 576 3.73e-29 111
MS.gene065760.t1 MTR_1g031510 46.729 107 56 1 1 107 483 588 3.95e-29 111
MS.gene065760.t1 MTR_3g028630 43.925 107 59 1 1 107 265 370 4.41e-29 109
MS.gene065760.t1 MTR_1g031460 48.598 107 54 1 1 107 458 563 4.93e-29 110
MS.gene065760.t1 MTR_1g027820 48.276 116 54 3 1 113 423 535 5.56e-29 110
MS.gene065760.t1 MTR_1g027970 45.946 111 58 2 1 111 456 564 1.26e-28 109
MS.gene065760.t1 MTR_1g027500 45.794 107 55 1 1 107 470 573 2.06e-28 109
MS.gene065760.t1 MTR_1g031560 49.515 103 49 2 1 102 501 601 2.88e-28 108
MS.gene065760.t1 MTR_1g028020 42.056 107 62 0 1 107 313 419 3.57e-28 108
MS.gene065760.t1 MTR_6g045030 42.991 107 60 1 1 107 274 379 3.71e-28 107
MS.gene065760.t1 MTR_1g028100 41.121 107 63 0 1 107 255 361 3.75e-28 107
MS.gene065760.t1 MTR_1g108910 49.057 106 37 1 2 107 209 297 6.71e-28 105
MS.gene065760.t1 MTR_1g031520 46.078 102 54 1 1 102 480 580 6.89e-28 107
MS.gene065760.t1 MTR_1g027410 46.296 108 55 2 1 107 267 372 9.62e-28 105
MS.gene065760.t1 MTR_1g027370 43.103 116 64 1 1 116 470 583 9.70e-28 107
MS.gene065760.t1 MTR_1g031200 46.729 107 55 2 1 107 367 471 1.51e-27 106
MS.gene065760.t1 MTR_1g027840 42.056 107 61 1 1 107 194 299 2.46e-27 104
MS.gene065760.t1 MTR_1g027420 43.103 116 63 1 1 116 413 525 3.09e-27 105
MS.gene065760.t1 MTR_1g028170 41.121 107 63 0 1 107 282 388 3.42e-27 105
MS.gene065760.t1 MTR_1g027070 41.284 109 64 0 1 109 564 672 3.83e-27 105
MS.gene065760.t1 MTR_1g027700 48.246 114 54 3 1 112 200 310 3.98e-27 103
MS.gene065760.t1 MTR_1g027040 43.846 130 62 4 1 127 427 548 4.54e-27 105
MS.gene065760.t1 MTR_1g027930 41.121 107 62 1 1 107 138 243 4.64e-27 102
MS.gene065760.t1 MTR_1g027060 43.411 129 63 4 1 127 372 492 5.03e-27 105
MS.gene065760.t1 MTR_1g027960 45.455 121 61 3 1 119 444 561 5.22e-27 105
MS.gene065760.t1 MTR_1g026940 47.664 107 52 2 1 107 469 571 6.19e-27 105
MS.gene065760.t1 MTR_1g027830 42.056 107 61 1 1 107 521 626 7.89e-27 104
MS.gene065760.t1 MTR_1g027890 42.500 120 65 2 1 119 269 385 1.72e-26 102
MS.gene065760.t1 MTR_1g027730 41.121 107 62 1 1 107 194 299 1.86e-26 102
MS.gene065760.t1 MTR_3g028650 45.455 121 61 3 1 119 425 542 2.33e-26 103
MS.gene065760.t1 MTR_1g027050 41.121 107 63 0 1 107 601 707 2.93e-26 103
MS.gene065760.t1 MTR_1g027140 41.121 107 63 0 1 107 598 704 3.84e-26 102
MS.gene065760.t1 MTR_0690s0010 42.149 121 64 3 1 119 404 520 4.14e-26 102
MS.gene065760.t1 MTR_7g060570 47.170 106 53 2 2 107 507 609 6.37e-26 102
MS.gene065760.t1 MTR_6g445600 42.149 121 64 3 1 119 430 546 9.59e-26 101
MS.gene065760.t1 MTR_1g027030 36.364 143 87 3 1 142 550 689 2.46e-25 100
MS.gene065760.t1 MTR_1g027030 36.364 143 87 3 1 142 600 739 2.51e-25 100
MS.gene065760.t1 MTR_1g028220 44.628 121 62 3 1 119 427 544 3.24e-25 100
MS.gene065760.t1 MTR_1g027150 46.364 110 52 4 1 107 425 530 2.77e-24 97.4
MS.gene065760.t1 MTR_1g110160 43.564 101 42 1 7 107 466 551 1.78e-23 95.1
MS.gene065760.t1 MTR_1g027200 42.400 125 60 3 1 125 399 511 1.08e-22 92.8
MS.gene065760.t1 MTR_4g073220 43.119 109 54 4 1 107 481 583 1.56e-21 89.7
MS.gene065760.t1 MTR_1g027440 38.583 127 59 4 1 125 446 555 5.38e-21 88.2
MS.gene065760.t1 MTR_2g036460 40.000 135 66 7 2 132 310 433 2.72e-20 85.9
MS.gene065760.t1 MTR_2g036440 38.168 131 70 5 2 130 242 363 4.65e-20 85.1
MS.gene065760.t1 MTR_2g036500 38.168 131 70 5 2 130 241 362 1.62e-19 83.6
MS.gene065760.t1 MTR_2g036490 38.636 132 68 6 2 130 300 421 3.35e-19 82.8
MS.gene065760.t1 MTR_1g099400 40.441 136 60 8 2 130 689 810 2.23e-18 80.9
MS.gene065760.t1 MTR_1g031400 53.226 62 29 0 1 62 186 247 4.11e-18 78.2
MS.gene065760.t1 MTR_1g031470 46.575 73 38 1 36 108 4 75 2.24e-17 73.2
MS.gene065760.t1 MTR_2g073250 43.363 113 50 7 2 107 651 756 1.54e-16 75.5
MS.gene065760.t1 MTR_4g113710 33.544 158 82 8 2 139 274 428 5.97e-16 73.6
MS.gene065760.t1 MTR_2g080080 40.909 110 57 5 2 107 647 752 6.14e-16 73.9
MS.gene065760.t1 MTR_3g107070 41.071 112 53 6 2 107 639 743 7.04e-16 73.6
MS.gene065760.t1 MTR_2g036430 34.351 131 75 5 2 130 225 346 8.58e-16 73.2
MS.gene065760.t1 MTR_2g054870 34.810 158 75 7 2 136 276 428 9.06e-16 73.2
MS.gene065760.t1 MTR_6g043510 41.071 112 54 6 2 107 656 761 2.05e-15 72.4
MS.gene065760.t1 MTR_1g031220 60.000 50 19 1 1 50 36 84 2.14e-15 67.4
MS.gene065760.t1 MTR_2g080100 39.091 110 60 4 2 107 615 721 3.07e-15 71.6
MS.gene065760.t1 MTR_4g093070 36.283 113 66 3 1 110 513 622 3.59e-15 71.6
MS.gene065760.t1 MTR_4g114270 42.574 101 51 4 2 100 691 786 6.45e-15 70.9
MS.gene065760.t1 MTR_6g043790 40.909 110 57 6 2 107 661 766 1.81e-14 69.7
MS.gene065760.t1 MTR_2g036480 36.752 117 66 5 2 115 258 369 1.88e-14 69.3
MS.gene065760.t1 MTR_4g114250 39.604 101 54 4 2 100 693 788 2.00e-14 69.3
MS.gene065760.t1 MTR_8g030500 36.364 121 58 5 1 107 648 763 3.51e-14 68.6
MS.gene065760.t1 MTR_8g028065 34.653 101 63 2 1 100 737 835 2.05e-13 66.6
MS.gene065760.t1 MTR_1g012550 38.393 112 56 7 2 107 667 771 4.28e-13 65.5
MS.gene065760.t1 MTR_0280s0040 37.168 113 57 7 2 107 659 764 1.59e-12 63.9
MS.gene065760.t1 MTR_6g057750 37.273 110 64 4 1 108 754 860 2.63e-12 63.5
MS.gene065760.t1 MTR_6g463700 37.273 110 64 4 1 108 709 815 2.64e-12 63.2
MS.gene065760.t1 MTR_2g089440 36.283 113 58 5 2 107 434 539 2.67e-12 63.2
MS.gene065760.t1 MTR_2g089440 36.283 113 58 5 2 107 666 771 4.24e-12 62.8
MS.gene065760.t1 MTR_2g090410 32.800 125 78 4 1 124 513 632 6.00e-12 62.4
MS.gene065760.t1 MTR_8g052170 39.823 113 63 4 2 112 204 313 6.42e-12 62.0
MS.gene065760.t1 MTR_2g103810 40.367 109 58 5 2 107 659 763 7.44e-12 62.0
MS.gene065760.t1 MTR_5g033690 33.858 127 72 4 1 119 476 598 8.11e-12 62.0
MS.gene065760.t1 MTR_8g052080 39.823 113 63 4 2 112 653 762 1.08e-11 61.6
MS.gene065760.t1 MTR_2g089360 37.168 113 57 6 2 107 661 766 1.16e-11 61.6
MS.gene065760.t1 MTR_8g461110 35.000 120 72 4 1 118 853 968 1.25e-11 61.6
MS.gene065760.t1 MTR_2g067990 32.174 115 66 5 2 111 883 990 1.40e-11 61.2
MS.gene065760.t1 MTR_2g068650 32.174 115 66 5 2 111 621 728 1.95e-11 60.8
MS.gene065760.t1 MTR_8g052490 36.667 120 57 3 2 112 231 340 2.58e-11 60.5
MS.gene065760.t1 MTR_8g052050 38.739 111 63 4 2 110 652 759 3.72e-11 60.1
MS.gene065760.t1 MTR_7g115740 32.759 116 67 4 1 111 524 633 3.96e-11 60.1
MS.gene065760.t1 MTR_7g115740 32.759 116 67 4 1 111 448 557 4.09e-11 59.7
MS.gene065760.t1 MTR_8g052570 38.053 113 65 4 2 112 230 339 6.34e-11 59.3
MS.gene065760.t1 MTR_8g052517 38.053 113 65 4 2 112 230 339 6.34e-11 59.3
MS.gene065760.t1 MTR_8g052290 38.053 113 65 4 2 112 257 366 8.34e-11 58.9
MS.gene065760.t1 MTR_8g445800 35.000 120 72 4 1 118 720 835 8.71e-11 58.9
MS.gene065760.t1 MTR_8g445800 35.000 120 72 4 1 118 865 980 8.83e-11 58.9
MS.gene065760.t1 MTR_8g445800 35.000 120 72 4 1 118 667 782 9.81e-11 58.9
MS.gene065760.t1 MTR_8g052190 38.053 113 65 4 2 112 645 754 9.94e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065760.t1 AT1G66980 54.206 107 43 3 1 102 952 1057 3.01e-32 120
MS.gene065760.t1 AT1G66980 54.206 107 43 3 1 102 969 1074 3.04e-32 120
MS.gene065760.t1 AT1G66980 54.206 107 43 3 1 102 998 1103 3.29e-32 120
MS.gene065760.t1 AT1G66980 54.206 107 43 3 1 102 996 1101 3.59e-32 120
MS.gene065760.t1 AT1G70250 50.893 112 49 3 1 107 624 734 4.57e-32 120
MS.gene065760.t1 AT1G67000 51.304 115 42 3 1 102 721 834 4.99e-31 117
MS.gene065760.t1 AT5G38240 51.351 111 49 4 1 107 429 538 2.37e-30 114
MS.gene065760.t1 AT4G18250 50.893 112 50 3 1 107 521 632 2.55e-30 114
MS.gene065760.t1 AT5G38240 53.774 106 44 4 1 102 493 597 2.95e-30 114
MS.gene065760.t1 AT5G38240 53.774 106 44 4 1 102 475 579 3.11e-30 114
MS.gene065760.t1 AT1G66920 52.294 109 49 2 1 107 472 579 4.07e-30 114
MS.gene065760.t1 AT1G66920 52.294 109 49 2 1 107 446 553 4.19e-30 114
MS.gene065760.t1 AT1G66920 52.294 109 49 2 1 107 464 571 5.00e-30 114
MS.gene065760.t1 AT5G39030 48.182 110 54 2 1 107 662 771 1.11e-29 113
MS.gene065760.t1 AT5G39030 48.182 110 54 2 1 107 656 765 1.30e-29 112
MS.gene065760.t1 AT1G66910 51.429 105 48 2 1 102 509 613 2.38e-29 112
MS.gene065760.t1 AT1G66910 51.429 105 48 2 1 102 512 616 2.46e-29 112
MS.gene065760.t1 AT5G38250 51.887 106 46 4 1 102 472 576 3.47e-29 111
MS.gene065760.t1 AT5G38280 46.847 111 54 3 1 107 496 605 3.96e-29 111
MS.gene065760.t1 AT5G38280 46.847 111 54 3 1 107 496 605 4.03e-29 111
MS.gene065760.t1 AT5G38260 50.476 105 49 2 1 102 485 589 1.36e-28 109
MS.gene065760.t1 AT5G39020 46.296 108 57 1 1 107 660 767 1.51e-28 109
MS.gene065760.t1 AT1G66930 49.533 107 49 2 1 102 511 617 2.26e-26 103
MS.gene065760.t1 AT1G66930 49.533 107 49 2 1 102 480 586 2.56e-26 103
MS.gene065760.t1 AT1G66930 49.533 107 49 2 1 102 720 826 2.91e-26 103
MS.gene065760.t1 AT5G24080 41.912 136 57 8 2 130 570 690 1.56e-18 81.3
MS.gene065760.t1 AT5G24080 41.912 136 57 8 2 130 570 690 1.56e-18 81.3
MS.gene065760.t1 AT5G24080 41.912 136 57 8 2 130 697 817 1.70e-18 81.3
MS.gene065760.t1 AT4G32300 44.545 110 53 6 2 107 659 764 2.41e-18 80.9
MS.gene065760.t1 AT1G34300 38.938 113 56 5 2 107 650 756 1.02e-14 70.5
MS.gene065760.t1 AT4G00340 36.441 118 58 5 2 107 647 759 5.67e-14 68.2
MS.gene065760.t1 AT4G00340 36.441 118 58 5 2 107 626 738 6.23e-14 68.2
MS.gene065760.t1 AT2G19130 38.739 111 59 5 2 107 660 766 7.81e-14 67.8
MS.gene065760.t1 AT4G04540 38.000 100 56 3 2 101 520 613 2.17e-13 66.6
MS.gene065760.t1 AT4G04540 38.000 100 56 3 2 101 491 584 2.45e-13 66.2
MS.gene065760.t1 AT5G20050 33.333 120 58 4 2 107 277 388 2.27e-12 63.5
MS.gene065760.t1 AT4G04570 35.965 114 64 4 2 115 515 619 3.18e-12 63.2
MS.gene065760.t1 AT4G04490 34.746 118 63 4 2 115 507 614 1.08e-11 61.6
MS.gene065760.t1 AT2G48010 32.110 109 69 3 1 107 452 557 5.59e-11 59.7

Find 32 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGAGTCTTCTAAGTCTTAA+AGG 0.208362 6.2:-82154432 MS.gene065760:CDS
AGCTTCACAAGATGATTAAA+AGG 0.244078 6.2:+82154337 None:intergenic
AATCATCTTGTGAAGCTATT+AGG 0.256175 6.2:-82154331 MS.gene065760:CDS
GAATGACAATTGTGGGTTTA+TGG 0.269136 6.2:-82154579 MS.gene065760:CDS
AAATGTCTATTCCACACTTC+TGG 0.331819 6.2:+82154805 None:intergenic
GAAGTGTGGAATAGACATTT+TGG 0.335930 6.2:-82154802 MS.gene065760:CDS
TCAATCACTTTACTCATTGT+AGG 0.342711 6.2:+82154529 None:intergenic
AAGTCTGATGTTTATAGTTA+TGG 0.356517 6.2:-82154766 MS.gene065760:CDS
TTCCACATTGGGTATACAAT+AGG 0.363248 6.2:-82154669 MS.gene065760:CDS
TTTGAGACCTGATGGAGTTA+TGG 0.423088 6.2:-82154629 MS.gene065760:CDS
GCAAGGAGAATGACAATTGT+GGG 0.440757 6.2:-82154586 MS.gene065760:CDS
TGGAATGATGCTTCTAGAAA+TGG 0.450669 6.2:-82154746 MS.gene065760:CDS
TTCAAACATTTCCAAGTGAT+AGG 0.452091 6.2:-82154552 MS.gene065760:CDS
CTCATTGTAGGCCTATCACT+TGG 0.456559 6.2:+82154541 None:intergenic
TGAGATATACTTTCCACATT+GGG 0.458771 6.2:-82154680 MS.gene065760:CDS
TCTGTATCCATAACTCCATC+AGG 0.481369 6.2:+82154622 None:intergenic
GTGAGATATACTTTCCACAT+TGG 0.531157 6.2:-82154681 MS.gene065760:CDS
GGCACTAATTTGAGACCTGA+TGG 0.536783 6.2:-82154637 MS.gene065760:CDS
GCTTCACAAGATGATTAAAA+GGG 0.545897 6.2:+82154338 None:intergenic
GAGATTGTCATCATTTGAAG+TGG 0.552747 6.2:+82154363 None:intergenic
TGCAAGGAGAATGACAATTG+TGG 0.556123 6.2:-82154587 MS.gene065760:CDS
ATGCTTCTAGAAATGGTTGG+AGG 0.562070 6.2:-82154739 MS.gene065760:CDS
GTATACAATAGGCTTGAGCT+TGG 0.571113 6.2:-82154658 MS.gene065760:CDS
CAGAAGAAGATGAGATTGCA+AGG 0.572000 6.2:-82154603 MS.gene065760:CDS
CTTCTAAGTCTTAAAGGAGA+GGG 0.595765 6.2:-82154426 MS.gene065760:intron
TCACAAGATGATTAAAAGGG+TGG 0.596422 6.2:+82154341 None:intergenic
TGCTTCTAGAAATGGTTGGA+GGG 0.598653 6.2:-82154738 MS.gene065760:CDS
TCTTCTAAGTCTTAAAGGAG+AGG 0.608683 6.2:-82154427 MS.gene065760:CDS
AGCCTATTGTATACCCAATG+TGG 0.611017 6.2:+82154667 None:intergenic
GATATGTAGCTCCAGAAGTG+TGG 0.626015 6.2:-82154816 MS.gene065760:CDS
TTCTAGAAATGGTTGGAGGG+AGG 0.644218 6.2:-82154735 MS.gene065760:CDS
ATGATGCTTCTAGAAATGGT+TGG 0.661502 6.2:-82154742 MS.gene065760:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAGTCTGATGTTTATAGTTA+TGG - chr6.2:82154354-82154373 MS.gene065760:CDS 25.0%
AATCATCTTGTGAAGCTATT+AGG - chr6.2:82154789-82154808 MS.gene065760:CDS 30.0%
AGCTTCACAAGATGATTAAA+AGG + chr6.2:82154786-82154805 None:intergenic 30.0%
GCTTCACAAGATGATTAAAA+GGG + chr6.2:82154785-82154804 None:intergenic 30.0%
TCAATCACTTTACTCATTGT+AGG + chr6.2:82154594-82154613 None:intergenic 30.0%
TGAGATATACTTTCCACATT+GGG - chr6.2:82154440-82154459 MS.gene065760:CDS 30.0%
TTCAAACATTTCCAAGTGAT+AGG - chr6.2:82154568-82154587 MS.gene065760:CDS 30.0%
AAATGTCTATTCCACACTTC+TGG + chr6.2:82154318-82154337 None:intergenic 35.0%
CAGAGTCTTCTAAGTCTTAA+AGG - chr6.2:82154688-82154707 MS.gene065760:CDS 35.0%
CTTCTAAGTCTTAAAGGAGA+GGG - chr6.2:82154694-82154713 MS.gene065760:CDS 35.0%
GAATGACAATTGTGGGTTTA+TGG - chr6.2:82154541-82154560 MS.gene065760:intron 35.0%
GAGATTGTCATCATTTGAAG+TGG + chr6.2:82154760-82154779 None:intergenic 35.0%
GTGAGATATACTTTCCACAT+TGG - chr6.2:82154439-82154458 MS.gene065760:CDS 35.0%
TCACAAGATGATTAAAAGGG+TGG + chr6.2:82154782-82154801 None:intergenic 35.0%
TCTTCTAAGTCTTAAAGGAG+AGG - chr6.2:82154693-82154712 MS.gene065760:CDS 35.0%
TTCCACATTGGGTATACAAT+AGG - chr6.2:82154451-82154470 MS.gene065760:intron 35.0%
! ATGATGCTTCTAGAAATGGT+TGG - chr6.2:82154378-82154397 MS.gene065760:intron 35.0%
! GAAGTGTGGAATAGACATTT+TGG - chr6.2:82154318-82154337 MS.gene065760:CDS 35.0%
! TGGAATGATGCTTCTAGAAA+TGG - chr6.2:82154374-82154393 MS.gene065760:CDS 35.0%
AGCCTATTGTATACCCAATG+TGG + chr6.2:82154456-82154475 None:intergenic 40.0%
AGGAGAAGAAAGCAATGGTT+TGG + chr6.2:82154655-82154674 None:intergenic 40.0%
CAGAAGAAGATGAGATTGCA+AGG - chr6.2:82154517-82154536 MS.gene065760:intron 40.0%
CATTGCTTTCTTCTCCTACT+AGG - chr6.2:82154658-82154677 MS.gene065760:CDS 40.0%
CTAGTAGGAGAAGAAAGCAA+TGG + chr6.2:82154660-82154679 None:intergenic 40.0%
GACTCTGATATTGACCTAGT+AGG + chr6.2:82154675-82154694 None:intergenic 40.0%
GCAAGGAGAATGACAATTGT+GGG - chr6.2:82154534-82154553 MS.gene065760:intron 40.0%
GGAGAAGAAAGCAATGGTTT+GGG + chr6.2:82154654-82154673 None:intergenic 40.0%
GTATACAATAGGCTTGAGCT+TGG - chr6.2:82154462-82154481 MS.gene065760:intron 40.0%
TCTGTATCCATAACTCCATC+AGG + chr6.2:82154501-82154520 None:intergenic 40.0%
TGCAAGGAGAATGACAATTG+TGG - chr6.2:82154533-82154552 MS.gene065760:intron 40.0%
! ATGCTTCTAGAAATGGTTGG+AGG - chr6.2:82154381-82154400 MS.gene065760:intron 40.0%
! GTGTGGAATAGACATTTTGG+AGG - chr6.2:82154321-82154340 MS.gene065760:CDS 40.0%
! TGCTTCTAGAAATGGTTGGA+GGG - chr6.2:82154382-82154401 MS.gene065760:intron 40.0%
! TTTGAGACCTGATGGAGTTA+TGG - chr6.2:82154491-82154510 MS.gene065760:intron 40.0%
CTCATTGTAGGCCTATCACT+TGG + chr6.2:82154582-82154601 None:intergenic 45.0%
GATATGTAGCTCCAGAAGTG+TGG - chr6.2:82154304-82154323 MS.gene065760:CDS 45.0%
! GAAGAAAGCAATGGTTTGGG+TGG + chr6.2:82154651-82154670 None:intergenic 45.0%
! GGCACTAATTTGAGACCTGA+TGG - chr6.2:82154483-82154502 MS.gene065760:intron 45.0%
! TTCTAGAAATGGTTGGAGGG+AGG - chr6.2:82154385-82154404 MS.gene065760:intron 45.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 82154300 82154842 82154300 ID=MS.gene065760
chr6.2 mRNA 82154300 82154842 82154300 ID=MS.gene065760.t1;Parent=MS.gene065760
chr6.2 exon 82154542 82154842 82154542 ID=MS.gene065760.t1.exon1;Parent=MS.gene065760.t1
chr6.2 CDS 82154542 82154842 82154542 ID=cds.MS.gene065760.t1;Parent=MS.gene065760.t1
chr6.2 exon 82154427 82154462 82154427 ID=MS.gene065760.t1.exon2;Parent=MS.gene065760.t1
chr6.2 CDS 82154427 82154462 82154427 ID=cds.MS.gene065760.t1;Parent=MS.gene065760.t1
chr6.2 exon 82154300 82154394 82154300 ID=MS.gene065760.t1.exon3;Parent=MS.gene065760.t1
chr6.2 CDS 82154300 82154394 82154300 ID=cds.MS.gene065760.t1;Parent=MS.gene065760.t1
Gene Sequence

>MS.gene065760

ATGGGATATGTAGCTCCAGAAGTGTGGAATAGACATTTTGGAGGAGTTTCACACAAGTCTGATGTTTATAGTTATGGAATGATGCTTCTAGAAATGGTTGGAGGGAGGAAAAACATTATTGCTGATGCAAGTCACACAAGTGAGATATACTTTCCACATTGGGTATACAATAGGCTTGAGCTTGGCACTAATTTGAGACCTGATGGAGTTATGGATACAGAAGAAGATGAGATTGCAAGGAGAATGACAATTGTGGGTTTATGGTGCATTCAAACATTTCCAAGTGATAGGCCTACAATGAGTAAAGTGATTGAAATGTTAGAAGTTAACATGAATTCACTTGAAATACCACCCAAACCATTGCTTTCTTCTCCTACTAGGTCAATATCAGAGTCTTCTAAGTCTTAAAGGAGAGGGTAAGTATAATTGTTATATATCTTTTAATCAGAGTCGATAACCACTTCAAATGATGACAATCTCCACCCTTTTAATCATCTTGTGAAGCTATTAGGTTTAAGTGTCTCAAATAGTTTGTACATTTAA
Protein sequence

>MS.gene065760.t1

MGYVAPEVWNRHFGGVSHKSDVYSYGMMLLEMVGGRKNIIADASHTSEIYFPHWVYNRLELGTNLRPDGVMDTEEDEIARRMTIVGLWCIQTFPSDRPTMSQYQSLLSLKGEESITTSNDDNLHPFNHLVKLLGLSVSNSLYI