Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065950.t1 | XP_024630632.1 | 99.2 | 127 | 1 | 0 | 8 | 134 | 1 | 127 | 6.10E-66 | 260 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065950.t1 | P0DPD7 | 35.4 | 127 | 73 | 2 | 11 | 129 | 70 | 195 | 4.5e-17 | 89.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065950.t1 | A0A396I324 | 99.2 | 124 | 1 | 0 | 11 | 134 | 61 | 184 | 4.1e-64 | 253.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052896 | MS.gene065950 | 0.810821 | 9.53E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGGGATATGAGCTTCTTTC+CGG | 0.249268 | 4.1:+58528470 | MS.gene065950:CDS |
| AGGAACTCTTGATTCATTAA+TGG | 0.250105 | 4.1:+58528658 | MS.gene065950:intron |
| TAGATTTGCAGATGGATGTT+AGG | 0.316176 | 4.1:+58528451 | MS.gene065950:intron |
| TTTGATGGTGTAATTGATAA+AGG | 0.329452 | 4.1:+58528501 | MS.gene065950:CDS |
| AGCATCTGAGAAGCACTAAT+TGG | 0.333558 | 4.1:-58528786 | None:intergenic |
| TGCAGTTATATCGGAGGATA+TGG | 0.336489 | 4.1:+58528232 | MS.gene065950:CDS |
| TAAGCAAACCTGTATACAAT+TGG | 0.406097 | 4.1:+58529097 | MS.gene065950:CDS |
| AGATTTGCAGATGGATGTTA+GGG | 0.451225 | 4.1:+58528452 | MS.gene065950:intron |
| CGTCTCTTGCAGATTACATA+TGG | 0.451583 | 4.1:+58529048 | MS.gene065950:intron |
| AATTGTATACAGGTTTGCTT+AGG | 0.468189 | 4.1:-58529095 | None:intergenic |
| TATGGTCAAAGATGGTTATG+AGG | 0.486363 | 4.1:+58528250 | MS.gene065950:CDS |
| GAAAATTTACAAGGAGCAAA+CGG | 0.493433 | 4.1:+58528201 | None:intergenic |
| ACACCATCAAAAGATTCATC+CGG | 0.525816 | 4.1:-58528489 | None:intergenic |
| ACCTGGAGGGACTTATATGT+TGG | 0.559844 | 4.1:+58528938 | MS.gene065950:CDS |
| TATACAGGTTTGCTTAGGTG+AGG | 0.565749 | 4.1:-58529090 | None:intergenic |
| ACCAACATATAAGTCCCTCC+AGG | 0.570329 | 4.1:-58528939 | None:intergenic |
| TCGGAGGATATGGTCAAAGA+TGG | 0.576243 | 4.1:+58528242 | MS.gene065950:CDS |
| AGGTGAGGCATCCGTACTGT+TGG | 0.584528 | 4.1:-58529075 | None:intergenic |
| AATCTCAATAGATTTGCAGA+TGG | 0.610505 | 4.1:+58528443 | MS.gene065950:intron |
| GCAGACTTCTAAAACCTGGA+GGG | 0.617930 | 4.1:+58528925 | MS.gene065950:intron |
| TGCAGACTTCTAAAACCTGG+AGG | 0.625568 | 4.1:+58528924 | MS.gene065950:intron |
| CATATGGTGATCCAACAGTA+CGG | 0.631426 | 4.1:+58529064 | MS.gene065950:CDS |
| AGATGCTTGCAGAAGTGTGT+AGG | 0.643334 | 4.1:+58528802 | MS.gene065950:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACAAAAAAAAAAAGTATAAT+TGG | - | chr4.1:58528902-58528921 | None:intergenic | 10.0% |
| !! | AAAAAAGAAAAGAAAATTTG+AGG | - | chr4.1:58528742-58528761 | None:intergenic | 15.0% |
| !! | AACAAGTAAAATAATGTTAT+TGG | - | chr4.1:58528361-58528380 | None:intergenic | 15.0% |
| !! | CAAATCTATAATTATATACA+TGG | + | chr4.1:58528565-58528584 | MS.gene065950:intron | 15.0% |
| !!! | AATAATTTAAGATATGTCAA+TGG | - | chr4.1:58528418-58528437 | None:intergenic | 15.0% |
| !!! | TTCTTTTTTGTATTTTAGTG+TGG | + | chr4.1:58528753-58528772 | MS.gene065950:intron | 20.0% |
| ! | AGATTTGATTAACTTCGAAA+AGG | - | chr4.1:58528552-58528571 | None:intergenic | 25.0% |
| !! | GATGTATTTTGCAGTTATAT+CGG | + | chr4.1:58528223-58528242 | MS.gene065950:CDS | 25.0% |
| !! | GTAATTTTCCAATTGTATAC+AGG | - | chr4.1:58529108-58529127 | None:intergenic | 25.0% |
| !!! | ATGTTGGTAATCTCATTTAA+TGG | + | chr4.1:58528954-58528973 | MS.gene065950:intron | 25.0% |
| !!! | TTTGATGGTGTAATTGATAA+AGG | + | chr4.1:58528501-58528520 | MS.gene065950:CDS | 25.0% |
| !!! | TTTTTTTCTCATCATGTCAA+TGG | - | chr4.1:58528306-58528325 | None:intergenic | 25.0% |
| AATCTCAATAGATTTGCAGA+TGG | + | chr4.1:58528443-58528462 | MS.gene065950:intron | 30.0% | |
| AGGAACTCTTGATTCATTAA+TGG | + | chr4.1:58528658-58528677 | MS.gene065950:intron | 30.0% | |
| CTCTTGATTCATTAATGGTA+TGG | + | chr4.1:58528663-58528682 | MS.gene065950:intron | 30.0% | |
| TAAGCAAACCTGTATACAAT+TGG | + | chr4.1:58529097-58529116 | MS.gene065950:CDS | 30.0% | |
| TGATTAACTTCGAAAAGGTA+CGG | - | chr4.1:58528547-58528566 | None:intergenic | 30.0% | |
| ! | AATTGTATACAGGTTTGCTT+AGG | - | chr4.1:58529098-58529117 | None:intergenic | 30.0% |
| ACACCATCAAAAGATTCATC+CGG | - | chr4.1:58528492-58528511 | None:intergenic | 35.0% | |
| AGATTTGCAGATGGATGTTA+GGG | + | chr4.1:58528452-58528471 | MS.gene065950:intron | 35.0% | |
| GGTGTAATTGATAAAGGTAG+TGG | + | chr4.1:58528507-58528526 | MS.gene065950:intron | 35.0% | |
| TAAACGATGAGAACAATCAG+TGG | - | chr4.1:58528693-58528712 | None:intergenic | 35.0% | |
| TAGATTTGCAGATGGATGTT+AGG | + | chr4.1:58528451-58528470 | MS.gene065950:intron | 35.0% | |
| TATGGTCAAAGATGGTTATG+AGG | + | chr4.1:58528250-58528269 | MS.gene065950:CDS | 35.0% | |
| TGCTATTGCATATACACTTG+TGG | + | chr4.1:58528620-58528639 | MS.gene065950:intron | 35.0% | |
| ! | CGTTTGCTCCTTGTAAATTT+TGG | - | chr4.1:58528203-58528222 | None:intergenic | 35.0% |
| ! | GTATTTTGCAGTTATATCGG+AGG | + | chr4.1:58528226-58528245 | MS.gene065950:CDS | 35.0% |
| ! | TTTCCGGATGAATCTTTTGA+TGG | + | chr4.1:58528486-58528505 | MS.gene065950:CDS | 35.0% |
| !!! | GGTAAGTACATTTTAGCTGA+GGG | - | chr4.1:58528340-58528359 | None:intergenic | 35.0% |
| !!! | TGGTAAGTACATTTTAGCTG+AGG | - | chr4.1:58528341-58528360 | None:intergenic | 35.0% |
| !!! | TTTTGCAGACTTCTAAAACC+TGG | + | chr4.1:58528921-58528940 | MS.gene065950:intron | 35.0% |
| CATATGGTGATCCAACAGTA+CGG | + | chr4.1:58529064-58529083 | MS.gene065950:CDS | 40.0% | |
| CGTCTCTTGCAGATTACATA+TGG | + | chr4.1:58529048-58529067 | MS.gene065950:intron | 40.0% | |
| TAGGGATATGAGCTTCTTTC+CGG | + | chr4.1:58528470-58528489 | MS.gene065950:CDS | 40.0% | |
| TCAAGAGTTCCTAGATGCAT+CGG | - | chr4.1:58528650-58528669 | None:intergenic | 40.0% | |
| TGCAGTTATATCGGAGGATA+TGG | + | chr4.1:58528232-58528251 | MS.gene065950:CDS | 40.0% | |
| ! | TATACAGGTTTGCTTAGGTG+AGG | - | chr4.1:58529093-58529112 | None:intergenic | 40.0% |
| !! | AGCATCTGAGAAGCACTAAT+TGG | - | chr4.1:58528789-58528808 | None:intergenic | 40.0% |
| ACCAACATATAAGTCCCTCC+AGG | - | chr4.1:58528942-58528961 | None:intergenic | 45.0% | |
| ACCTGGAGGGACTTATATGT+TGG | + | chr4.1:58528938-58528957 | MS.gene065950:CDS | 45.0% | |
| CAAGAGTTCCTAGATGCATC+GGG | - | chr4.1:58528649-58528668 | None:intergenic | 45.0% | |
| TCGGAGGATATGGTCAAAGA+TGG | + | chr4.1:58528242-58528261 | MS.gene065950:CDS | 45.0% | |
| ! | AGATGCTTGCAGAAGTGTGT+AGG | + | chr4.1:58528802-58528821 | MS.gene065950:CDS | 45.0% |
| !! | GCAGACTTCTAAAACCTGGA+GGG | + | chr4.1:58528925-58528944 | MS.gene065950:intron | 45.0% |
| !! | TGCAGACTTCTAAAACCTGG+AGG | + | chr4.1:58528924-58528943 | MS.gene065950:intron | 45.0% |
| TGTGGTATCCCGATGCATCT+AGG | + | chr4.1:58528638-58528657 | MS.gene065950:intron | 50.0% | |
| ! | AGGTGAGGCATCCGTACTGT+TGG | - | chr4.1:58529078-58529097 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 58528202 | 58529141 | 58528202 | ID=MS.gene065950 |
| chr4.1 | mRNA | 58528202 | 58529141 | 58528202 | ID=MS.gene065950.t1;Parent=MS.gene065950 |
| chr4.1 | exon | 58528202 | 58528350 | 58528202 | ID=MS.gene065950.t1.exon1;Parent=MS.gene065950.t1 |
| chr4.1 | CDS | 58528202 | 58528350 | 58528202 | ID=cds.MS.gene065950.t1;Parent=MS.gene065950.t1 |
| chr4.1 | exon | 58528454 | 58528522 | 58528454 | ID=MS.gene065950.t1.exon2;Parent=MS.gene065950.t1 |
| chr4.1 | CDS | 58528454 | 58528522 | 58528454 | ID=cds.MS.gene065950.t1;Parent=MS.gene065950.t1 |
| chr4.1 | exon | 58528660 | 58528679 | 58528660 | ID=MS.gene065950.t1.exon3;Parent=MS.gene065950.t1 |
| chr4.1 | CDS | 58528660 | 58528679 | 58528660 | ID=cds.MS.gene065950.t1;Parent=MS.gene065950.t1 |
| chr4.1 | exon | 58528771 | 58528823 | 58528771 | ID=MS.gene065950.t1.exon4;Parent=MS.gene065950.t1 |
| chr4.1 | CDS | 58528771 | 58528823 | 58528771 | ID=cds.MS.gene065950.t1;Parent=MS.gene065950.t1 |
| chr4.1 | exon | 58528929 | 58528959 | 58528929 | ID=MS.gene065950.t1.exon5;Parent=MS.gene065950.t1 |
| chr4.1 | CDS | 58528929 | 58528959 | 58528929 | ID=cds.MS.gene065950.t1;Parent=MS.gene065950.t1 |
| chr4.1 | exon | 58529060 | 58529141 | 58529060 | ID=MS.gene065950.t1.exon6;Parent=MS.gene065950.t1 |
| chr4.1 | CDS | 58529060 | 58529141 | 58529060 | ID=cds.MS.gene065950.t1;Parent=MS.gene065950.t1 |
| Gene Sequence |
| Protein sequence |
