AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065976

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065976.t1 MTR_8g087750 97.935 678 14 0 1 678 1 678 0.0 1357
MS.gene065976.t1 MTR_8g087770 81.967 671 112 5 11 677 6 671 0.0 1109
MS.gene065976.t1 MTR_8g087763 72.000 225 63 0 376 600 4 228 7.89e-118 356
MS.gene065976.t1 MTR_4g093750 38.272 324 180 7 69 378 35 352 1.10e-46 176
MS.gene065976.t1 MTR_4g093750 40.550 291 153 7 69 345 35 319 2.03e-46 176
MS.gene065976.t1 MTR_2g009690 38.488 291 167 6 65 345 40 328 4.53e-43 167
MS.gene065976.t1 MTR_8g105580 36.054 294 165 8 71 349 35 320 2.19e-34 139
MS.gene065976.t1 MTR_8g105580 36.054 294 165 8 71 349 35 320 2.38e-34 139
MS.gene065976.t1 MTR_5g009150 35.836 293 168 7 71 349 35 321 5.13e-34 138
MS.gene065976.t1 MTR_1g032550 32.865 356 197 12 71 392 35 382 9.79e-34 137
MS.gene065976.t1 MTR_7g451010 34.810 316 182 9 71 371 40 346 1.77e-33 136
MS.gene065976.t1 MTR_8g009510 35.127 316 181 9 71 371 40 346 6.75e-33 135
MS.gene065976.t1 MTR_4g098920 32.402 358 196 12 71 392 34 381 1.22e-31 130
MS.gene065976.t1 MTR_8g027675 42.211 199 104 5 71 266 34 224 1.33e-31 130
MS.gene065976.t1 MTR_8g099550 33.441 311 193 7 71 371 34 340 1.72e-31 130
MS.gene065976.t1 MTR_4g085860 72.368 76 20 1 208 282 2 77 2.59e-29 111
MS.gene065976.t1 MTR_6g045107 71.053 76 21 1 208 282 2 77 2.49e-27 105
MS.gene065976.t1 MTR_8g009510 31.707 246 148 6 138 371 2 239 2.61e-18 89.4
MS.gene065976.t1 MTR_8g463270 29.583 240 146 9 56 281 27 257 5.48e-15 79.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065976.t1 AT1G60500 59.438 641 256 3 35 673 29 667 0.0 794
MS.gene065976.t1 AT1G60530 71.181 288 77 1 22 309 19 300 3.92e-141 414
MS.gene065976.t1 AT4G33650 35.604 323 187 8 38 345 30 346 1.07e-42 166
MS.gene065976.t1 AT4G33650 35.604 323 187 8 38 345 30 346 1.16e-42 166
MS.gene065976.t1 AT2G14120 34.954 329 192 8 36 348 12 334 1.76e-41 162
MS.gene065976.t1 AT2G14120 34.954 329 192 8 36 348 12 334 1.76e-41 161
MS.gene065976.t1 AT2G14120 34.954 329 192 8 36 348 12 334 1.79e-41 162
MS.gene065976.t1 AT2G14120 34.954 329 192 8 36 348 12 334 2.22e-41 161
MS.gene065976.t1 AT1G14830 34.405 311 190 7 71 371 35 341 7.34e-36 144
MS.gene065976.t1 AT2G44590 32.915 319 197 8 71 378 35 347 1.19e-34 140
MS.gene065976.t1 AT3G60190 34.084 311 191 7 71 371 40 346 8.62e-34 137
MS.gene065976.t1 AT5G42080 34.727 311 189 7 71 371 34 340 8.01e-33 132
MS.gene065976.t1 AT5G42080 34.727 311 189 7 71 371 34 340 1.87e-32 133
MS.gene065976.t1 AT5G42080 34.727 311 189 7 71 371 34 340 1.87e-32 133
MS.gene065976.t1 AT5G42080 34.727 311 189 7 71 371 34 340 2.09e-32 133
MS.gene065976.t1 AT3G61760 34.177 316 184 9 71 371 34 340 2.63e-31 128
MS.gene065976.t1 AT3G61760 34.177 316 184 9 71 371 34 340 3.43e-31 129
MS.gene065976.t1 AT3G61760 34.177 316 184 9 71 371 34 340 3.66e-31 129
MS.gene065976.t1 AT2G44590 30.094 319 189 9 71 378 35 330 3.63e-25 110
MS.gene065976.t1 AT2G44590 30.094 319 189 9 71 378 35 330 4.13e-25 110
MS.gene065976.t1 AT1G53140 25.000 280 190 6 1 271 1 269 7.16e-15 79.0

Find 112 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAGTCTCCGTCTATTTC+TGG 0.150723 4.3:+13308251 MS.gene065976:CDS
AGTCTCACTCACTTCTTTCT+AGG 0.252212 4.3:+13307211 MS.gene065976:CDS
ATTCTGAAGGCGCATAATTA+AGG 0.255203 4.3:-13306650 None:intergenic
GCAGTTCTTGGCATGAAATT+TGG 0.255839 4.3:-13307642 None:intergenic
TTACTACTTGTGAGTCTATC+AGG 0.258224 4.3:+13306995 MS.gene065976:CDS
TGGAAGCTGAATTCCTTCTT+TGG 0.309988 4.3:-13306524 None:intergenic
TTCAAATTATTATCAGCTTC+AGG 0.310159 4.3:+13307782 MS.gene065976:CDS
TTCTCAAGGGCGTCGAGAAC+TGG 0.318424 4.3:-13306481 None:intergenic
AGTATTTGCTCGAGTATAAT+AGG 0.323700 4.3:+13307919 MS.gene065976:CDS
TTAACTTTGTTGATAATGTT+TGG 0.326087 4.3:+13307715 MS.gene065976:CDS
CTTCCAACAATTGTTGTTGT+TGG 0.331397 4.3:+13306541 MS.gene065976:CDS
GGAGACTGTTGCAAGAATTA+TGG 0.333112 4.3:+13308322 MS.gene065976:CDS
GTTGTTGGAGATCAATCTTC+AGG 0.340523 4.3:+13306556 MS.gene065976:CDS
TCACAAATGGTTGATAAAAC+TGG 0.343252 4.3:+13307021 MS.gene065976:CDS
TTTAGCTCAGAAGCTTGTTC+AGG 0.351831 4.3:+13307263 MS.gene065976:CDS
TTGAATTCTAACAAGAGTTC+TGG 0.352154 4.3:-13306688 None:intergenic
TTCAAGATAATACTTTCTTC+AGG 0.356152 4.3:-13306949 None:intergenic
CTTACCATGGTTGATCTTCC+AGG 0.356170 4.3:+13306847 MS.gene065976:CDS
TCAGATAAAAGATATTATTA+TGG 0.384953 4.3:+13306915 MS.gene065976:CDS
AGGCTAGGATGATTGCTTAC+TGG 0.386618 4.3:+13308087 MS.gene065976:CDS
AAGTTCAGTCCAACATGTTA+TGG 0.389392 4.3:+13307851 MS.gene065976:CDS
ACATTATCAACAAAGTTAAC+TGG 0.400837 4.3:-13307711 None:intergenic
ACCTTCTCCAACAATCCTTC+AGG 0.403379 4.3:-13307084 None:intergenic
CAAAGTTCTTAGAGAAAGTA+AGG 0.404659 4.3:+13308301 MS.gene065976:CDS
CATAGGTCTCATCTCCAATT+CGG 0.409654 4.3:-13307155 None:intergenic
TTAATCTTCTTGATGATCTC+TGG 0.410614 4.3:-13307315 None:intergenic
TCTCCAACAACAACAATTGT+TGG 0.419207 4.3:-13306544 None:intergenic
AATACTGCAGAGAAAAGTTA+AGG 0.423316 4.3:+13307674 MS.gene065976:CDS
GTCTGTGTGCGAAACCGAAT+TGG 0.426739 4.3:+13307141 MS.gene065976:CDS
TCGACGCCCTTGAGAATCTA+AGG 0.430085 4.3:+13306488 MS.gene065976:CDS
ATCTGATCATAGATATTGTC+AGG 0.431759 4.3:-13306898 None:intergenic
CAGGTTTCCACTCGCAGAGC+AGG 0.433533 4.3:+13307801 MS.gene065976:CDS
CCAACACATGTGAAGCTTGA+AGG 0.436407 4.3:+13307996 MS.gene065976:CDS
CAATGCATGTGCTTGTCCTC+AGG 0.447247 4.3:-13307489 None:intergenic
AGGATTGACAAATCCATTGT+TGG 0.451509 4.3:+13307231 MS.gene065976:CDS
TAAATCTCCTGAAGGATTGT+TGG 0.453191 4.3:+13307077 MS.gene065976:CDS
GACCTATGAAGATGCTAGAT+TGG 0.455034 4.3:+13307170 MS.gene065976:CDS
ATCACCCGTGTACCTGTTCA+TGG 0.456607 4.3:+13306871 MS.gene065976:CDS
GAACTCTTGTTAGAATTCAA+TGG 0.458145 4.3:+13306691 MS.gene065976:CDS
ATTTGGTAGACCAATCCATT+TGG 0.458705 4.3:-13307625 None:intergenic
ACAAGCTTCTGAGCTAAAAC+AGG 0.462376 4.3:-13307258 None:intergenic
TATGGAAGAGATTAAGGTGT+TGG 0.462977 4.3:+13307596 MS.gene065976:CDS
AGGAGTGTTGGAAATCCCTT+TGG 0.463701 4.3:-13306788 None:intergenic
TGATACCAGCTAAAGATTCA+AGG 0.464998 4.3:-13306591 None:intergenic
TTCTGAAGGCGCATAATTAA+GGG 0.465234 4.3:-13306649 None:intergenic
AGTGTCCTTGAATCTTTAGC+TGG 0.467085 4.3:+13306586 MS.gene065976:CDS
AAGAGTTCTGGCTTTGAAAG+TGG 0.480074 4.3:-13306676 None:intergenic
CCTTCAAGCTTCACATGTGT+TGG 0.480389 4.3:-13307996 None:intergenic
CGTCTCAACATTGCCAAAGA+AGG 0.485759 4.3:+13306511 MS.gene065976:CDS
CTCTAAGAACTTTGACACTT+CGG 0.488083 4.3:-13308292 None:intergenic
CTCCGTCTATTTCTGGAAAG+AGG 0.488105 4.3:+13308258 MS.gene065976:CDS
ATAGCTACTGAAGAGATTGC+TGG 0.488901 4.3:+13306760 MS.gene065976:CDS
TCATTGTAGGATGAAACAAT+TGG 0.490493 4.3:-13306448 None:intergenic
AAAGTGATGTTTCATCTCGT+TGG 0.495363 4.3:-13306381 None:intergenic
TTGTCAGGTTGACCATGAAC+AGG 0.495557 4.3:-13306883 None:intergenic
AGGTGTTGGAAGAGGCCAAA+TGG 0.496352 4.3:+13307610 MS.gene065976:CDS
ACAGGTACACGGGTGATACC+TGG 0.496559 4.3:-13306865 None:intergenic
TTACCCTCATGTGCTAAGTC+AGG 0.501551 4.3:+13308052 MS.gene065976:CDS
GCAACAGAAGATAAATTAGC+AGG 0.505796 4.3:-13307378 None:intergenic
GCAAGAATTATGGACAGGAT+TGG 0.508761 4.3:+13308332 MS.gene065976:CDS
AGATCAACCATGGTAAGATC+AGG 0.510480 4.3:-13306841 None:intergenic
AATCAAAGTCAAAGGAGTGT+TGG 0.514073 4.3:-13306800 None:intergenic
TTCCAATCTAGCATCTTCAT+AGG 0.522397 4.3:-13307172 None:intergenic
GTCTATCAGGATGTCACAAA+TGG 0.523985 4.3:+13307008 MS.gene065976:CDS
GATGACGTTAACATCGGTCT+AGG 0.524437 4.3:+13307114 MS.gene065976:CDS
CTCTCAATCCCACCACCACT+TGG 0.524638 4.3:-13308221 None:intergenic
TTGGAAGAGGCCAAATGGAT+TGG 0.525795 4.3:+13307615 MS.gene065976:CDS
AGAGATTAAGGTGTTGGAAG+AGG 0.526369 4.3:+13307602 MS.gene065976:CDS
GAGATTGCTGGTTCTGCCAA+AGG 0.527844 4.3:+13306772 MS.gene065976:CDS
TTCTTGACAATCAAAGTCAA+AGG 0.538928 4.3:-13306808 None:intergenic
ATCAGCTTGCCACGTGGACA+AGG 0.540401 4.3:+13306610 MS.gene065976:CDS
CTGTTGCAAGAATTATGGAC+AGG 0.542640 4.3:+13308327 MS.gene065976:CDS
AGATTGCTGGTTCTGCCAAA+GGG 0.550639 4.3:+13306773 MS.gene065976:CDS
GAGGTTGAGCTTGCTCAACA+TGG 0.552600 4.3:-13306411 None:intergenic
ATACTGCAGAGAAAAGTTAA+GGG 0.552698 4.3:+13307675 MS.gene065976:CDS
AATGCATGTGCTTGTCCTCA+GGG 0.555069 4.3:-13307488 None:intergenic
CAAGTGGTGGTGGGATTGAG+AGG 0.561054 4.3:+13308222 MS.gene065976:CDS
TGGTTGATAAAACTGGTTTG+AGG 0.562514 4.3:+13307028 MS.gene065976:CDS
ATTGGTGCAACAACAGAAAG+AGG 0.569115 4.3:-13306430 None:intergenic
TCCTGAAGGATTGTTGGAGA+AGG 0.571650 4.3:+13307083 MS.gene065976:CDS
AGCTAAAGATTCAAGGACAC+TGG 0.572468 4.3:-13306584 None:intergenic
TAAAATCAACAGTGGCGGAA+AGG 0.574096 4.3:-13306975 None:intergenic
TCCCTCTTTCCAGAAATAGA+CGG 0.574959 4.3:-13308260 None:intergenic
TCCGTCTATTTCTGGAAAGA+GGG 0.578371 4.3:+13308259 MS.gene065976:CDS
ACTGGAAGATTGTTCTGAGG+AGG 0.593317 4.3:+13308105 MS.gene065976:CDS
CAACAGCTTCCATAACATGT+TGG 0.595030 4.3:-13307860 None:intergenic
TGTTATGGAAGCTGTTGAGA+TGG 0.595595 4.3:+13307866 MS.gene065976:CDS
GATTAACAATGATTTGCAGA+AGG 0.597025 4.3:+13308175 MS.gene065976:CDS
AAAGCCTGACTTAGCACATG+AGG 0.603423 4.3:-13308056 None:intergenic
CTTACTGGAAGATTGTTCTG+AGG 0.604360 4.3:+13308102 MS.gene065976:CDS
TTGAAAGTGGATGATTCTGA+AGG 0.605131 4.3:-13306663 None:intergenic
GTTGACCATGAACAGGTACA+CGG 0.609991 4.3:-13306876 None:intergenic
TTTCTCGCCTGCTCTGCGAG+TGG 0.614106 4.3:-13307808 None:intergenic
TGAGACGCCTTAGATTCTCA+AGG 0.620323 4.3:-13306495 None:intergenic
AAAGCAGATAAATCTCCTGA+AGG 0.623780 4.3:+13307069 MS.gene065976:CDS
GAGACGCCTTAGATTCTCAA+GGG 0.625868 4.3:-13306494 None:intergenic
TAAAACAGGAATACCAACAA+TGG 0.629558 4.3:-13307244 None:intergenic
TTATACTCGAGCAAATACTC+AGG 0.634135 4.3:-13307915 None:intergenic
TTGACCATGAACAGGTACAC+GGG 0.647300 4.3:-13306875 None:intergenic
AAATTGATGTTGTTAATCTG+AGG 0.650762 4.3:+13308027 MS.gene065976:CDS
AAGCCTGACTTAGCACATGA+GGG 0.658164 4.3:-13308055 None:intergenic
AGTAGTAAAATCAACAGTGG+CGG 0.662087 4.3:-13306980 None:intergenic
GTGCAGATTCCTTGTCCACG+TGG 0.668352 4.3:-13306619 None:intergenic
CATGTGAAGCTTGAAGGTGT+TGG 0.674753 4.3:+13308002 MS.gene065976:CDS
TGGTGTTCCTGATCTTACCA+TGG 0.685138 4.3:+13306834 MS.gene065976:CDS
ACTGCTGATGACGTTAACAT+CGG 0.693473 4.3:+13307108 MS.gene065976:CDS
GCTGGTATCAGCTTGCCACG+TGG 0.693476 4.3:+13306604 MS.gene065976:CDS
GTGGACAAGGAATCTGCACG+AGG 0.706834 4.3:+13306623 MS.gene065976:CDS
ACAAGTAGTAAAATCAACAG+TGG 0.707970 4.3:-13306983 None:intergenic
GATACCTGGAAGATCAACCA+TGG 0.712735 4.3:-13306851 None:intergenic
TGGACAAGGAATCTGCACGA+GGG 0.740882 4.3:+13306624 MS.gene065976:CDS
TGATTTCGAAGAGTACCCTG+AGG 0.804578 4.3:+13307473 MS.gene065976:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAGATAAAAGATATTATTA+TGG + chr4.3:13306915-13306934 MS.gene065976:CDS 15.0%
!!! TTAACTTTGTTGATAATGTT+TGG + chr4.3:13307715-13307734 MS.gene065976:CDS 20.0%
! AAATTGATGTTGTTAATCTG+AGG + chr4.3:13308027-13308046 MS.gene065976:CDS 25.0%
! ACATTATCAACAAAGTTAAC+TGG - chr4.3:13307714-13307733 None:intergenic 25.0%
! ACTTTGATTGTCAAGAAAAA+TGG + chr4.3:13306814-13306833 MS.gene065976:CDS 25.0%
! TTCAAATTATTATCAGCTTC+AGG + chr4.3:13307782-13307801 MS.gene065976:CDS 25.0%
! TTCAAGATAATACTTTCTTC+AGG - chr4.3:13306952-13306971 None:intergenic 25.0%
!! TGCGAAAAAGAATTTTCTTA+TGG + chr4.3:13307578-13307597 MS.gene065976:CDS 25.0%
!! TTTTCTTATGGAAGAGATTA+AGG + chr4.3:13307590-13307609 MS.gene065976:CDS 25.0%
AATACTGCAGAGAAAAGTTA+AGG + chr4.3:13307674-13307693 MS.gene065976:CDS 30.0%
ACAAGTAGTAAAATCAACAG+TGG - chr4.3:13306986-13307005 None:intergenic 30.0%
AGTATTTGCTCGAGTATAAT+AGG + chr4.3:13307919-13307938 MS.gene065976:CDS 30.0%
ATACTGCAGAGAAAAGTTAA+GGG + chr4.3:13307675-13307694 MS.gene065976:CDS 30.0%
ATCTGATCATAGATATTGTC+AGG - chr4.3:13306901-13306920 None:intergenic 30.0%
ATTGTCAAAAATGCAGTTCT+TGG - chr4.3:13307657-13307676 None:intergenic 30.0%
CAAAGTTCTTAGAGAAAGTA+AGG + chr4.3:13308301-13308320 MS.gene065976:CDS 30.0%
CTCAAAAAGATTCTTCTTAC+AGG + chr4.3:13307450-13307469 MS.gene065976:CDS 30.0%
GAACTCTTGTTAGAATTCAA+TGG + chr4.3:13306691-13306710 MS.gene065976:CDS 30.0%
TAAAACAGGAATACCAACAA+TGG - chr4.3:13307247-13307266 None:intergenic 30.0%
TCATTGTAGGATGAAACAAT+TGG - chr4.3:13306451-13306470 None:intergenic 30.0%
TTCTTGACAATCAAAGTCAA+AGG - chr4.3:13306811-13306830 None:intergenic 30.0%
TTGAATTCTAACAAGAGTTC+TGG - chr4.3:13306691-13306710 None:intergenic 30.0%
! ACAGCTTTTATGCATATTCT+TGG + chr4.3:13307411-13307430 MS.gene065976:CDS 30.0%
! GATTAACAATGATTTGCAGA+AGG + chr4.3:13308175-13308194 MS.gene065976:CDS 30.0%
! TTAATCTTCTTGATGATCTC+TGG - chr4.3:13307318-13307337 None:intergenic 30.0%
!! TCACAAATGGTTGATAAAAC+TGG + chr4.3:13307021-13307040 MS.gene065976:CDS 30.0%
!!! TGGACGTATTTTTTCATTGT+AGG - chr4.3:13306464-13306483 None:intergenic 30.0%
AAAGCAGATAAATCTCCTGA+AGG + chr4.3:13307069-13307088 MS.gene065976:CDS 35.0%
AAAGTGATGTTTCATCTCGT+TGG - chr4.3:13306384-13306403 None:intergenic 35.0%
AAGTTCAGTCCAACATGTTA+TGG + chr4.3:13307851-13307870 MS.gene065976:CDS 35.0%
AGGATTGACAAATCCATTGT+TGG + chr4.3:13307231-13307250 MS.gene065976:CDS 35.0%
AGTAGTAAAATCAACAGTGG+CGG - chr4.3:13306983-13307002 None:intergenic 35.0%
ATTTGGTAGACCAATCCATT+TGG - chr4.3:13307628-13307647 None:intergenic 35.0%
GCAACAGAAGATAAATTAGC+AGG - chr4.3:13307381-13307400 None:intergenic 35.0%
TAAATCTCCTGAAGGATTGT+TGG + chr4.3:13307077-13307096 MS.gene065976:CDS 35.0%
TATGGAAGAGATTAAGGTGT+TGG + chr4.3:13307596-13307615 MS.gene065976:CDS 35.0%
TCTCCAACAACAACAATTGT+TGG - chr4.3:13306547-13306566 None:intergenic 35.0%
TGATACCAGCTAAAGATTCA+AGG - chr4.3:13306594-13306613 None:intergenic 35.0%
TTACTACTTGTGAGTCTATC+AGG + chr4.3:13306995-13307014 MS.gene065976:CDS 35.0%
TTATACTCGAGCAAATACTC+AGG - chr4.3:13307918-13307937 None:intergenic 35.0%
TTCCAATCTAGCATCTTCAT+AGG - chr4.3:13307175-13307194 None:intergenic 35.0%
TTGAAAGTGGATGATTCTGA+AGG - chr4.3:13306666-13306685 None:intergenic 35.0%
! AATCAAAGTCAAAGGAGTGT+TGG - chr4.3:13306803-13306822 None:intergenic 35.0%
! AATGATCTTTTGTCTCCAAG+TGG + chr4.3:13308206-13308225 MS.gene065976:CDS 35.0%
! ACTAACAGAAGCTTTTGTAG+TGG - chr4.3:13306356-13306375 None:intergenic 35.0%
! ATTCTGAAGGCGCATAATTA+AGG - chr4.3:13306653-13306672 None:intergenic 35.0%
! CTCTAAGAACTTTGACACTT+CGG - chr4.3:13308295-13308314 None:intergenic 35.0%
! CTTCCAACAATTGTTGTTGT+TGG + chr4.3:13306541-13306560 MS.gene065976:CDS 35.0%
! TTCTGAAGGCGCATAATTAA+GGG - chr4.3:13306652-13306671 None:intergenic 35.0%
!! TGGTTGATAAAACTGGTTTG+AGG + chr4.3:13307028-13307047 MS.gene065976:CDS 35.0%
!!! AAGTCAGGCTTTTGATTTGA+AGG + chr4.3:13308067-13308086 MS.gene065976:CDS 35.0%
ACAAGCTTCTGAGCTAAAAC+AGG - chr4.3:13307261-13307280 None:intergenic 40.0%
AGATCAACCATGGTAAGATC+AGG - chr4.3:13306844-13306863 None:intergenic 40.0%
AGCTAAAGATTCAAGGACAC+TGG - chr4.3:13306587-13306606 None:intergenic 40.0%
AGTCTCACTCACTTCTTTCT+AGG + chr4.3:13307211-13307230 MS.gene065976:CDS 40.0%
AGTGTCCTTGAATCTTTAGC+TGG + chr4.3:13306586-13306605 MS.gene065976:CDS 40.0%
ATAGCTACTGAAGAGATTGC+TGG + chr4.3:13306760-13306779 MS.gene065976:CDS 40.0%
ATTGGTGCAACAACAGAAAG+AGG - chr4.3:13306433-13306452 None:intergenic 40.0%
CAACAGCTTCCATAACATGT+TGG - chr4.3:13307863-13307882 None:intergenic 40.0%
CATAGGTCTCATCTCCAATT+CGG - chr4.3:13307158-13307177 None:intergenic 40.0%
CTGTTGCAAGAATTATGGAC+AGG + chr4.3:13308327-13308346 MS.gene065976:CDS 40.0%
CTTACTGGAAGATTGTTCTG+AGG + chr4.3:13308102-13308121 MS.gene065976:CDS 40.0%
GACCTATGAAGATGCTAGAT+TGG + chr4.3:13307170-13307189 MS.gene065976:CDS 40.0%
GCAAGAATTATGGACAGGAT+TGG + chr4.3:13308332-13308351 MS.gene065976:CDS 40.0%
GCAGTTCTTGGCATGAAATT+TGG - chr4.3:13307645-13307664 None:intergenic 40.0%
GGAGACTGTTGCAAGAATTA+TGG + chr4.3:13308322-13308341 MS.gene065976:CDS 40.0%
GTCTATCAGGATGTCACAAA+TGG + chr4.3:13307008-13307027 MS.gene065976:CDS 40.0%
GTTGTTGGAGATCAATCTTC+AGG + chr4.3:13306556-13306575 MS.gene065976:CDS 40.0%
TCCCTCTTTCCAGAAATAGA+CGG - chr4.3:13308263-13308282 None:intergenic 40.0%
TGGAAGCTGAATTCCTTCTT+TGG - chr4.3:13306527-13306546 None:intergenic 40.0%
TGTTATGGAAGCTGTTGAGA+TGG + chr4.3:13307866-13307885 MS.gene065976:CDS 40.0%
! ACTGCTGATGACGTTAACAT+CGG + chr4.3:13307108-13307127 MS.gene065976:CDS 40.0%
! TAAAATCAACAGTGGCGGAA+AGG - chr4.3:13306978-13306997 None:intergenic 40.0%
! TTTAGCTCAGAAGCTTGTTC+AGG + chr4.3:13307263-13307282 MS.gene065976:CDS 40.0%
!! AAGAGTTCTGGCTTTGAAAG+TGG - chr4.3:13306679-13306698 None:intergenic 40.0%
!! AGAGATTAAGGTGTTGGAAG+AGG + chr4.3:13307602-13307621 MS.gene065976:CDS 40.0%
!! TCCGTCTATTTCTGGAAAGA+GGG + chr4.3:13308259-13308278 MS.gene065976:CDS 40.0%
!!! AGGCTTTTGATTTGAAGGCT+AGG + chr4.3:13308072-13308091 MS.gene065976:CDS 40.0%
AAAGCCTGACTTAGCACATG+AGG - chr4.3:13308059-13308078 None:intergenic 45.0%
AAGCCTGACTTAGCACATGA+GGG - chr4.3:13308058-13308077 None:intergenic 45.0%
AATGCATGTGCTTGTCCTCA+GGG - chr4.3:13307491-13307510 None:intergenic 45.0%
ACCTTCTCCAACAATCCTTC+AGG - chr4.3:13307087-13307106 None:intergenic 45.0%
AGGCTAGGATGATTGCTTAC+TGG + chr4.3:13308087-13308106 MS.gene065976:CDS 45.0%
CCAACACATGTGAAGCTTGA+AGG + chr4.3:13307996-13308015 MS.gene065976:CDS 45.0%
CCTTCAAGCTTCACATGTGT+TGG - chr4.3:13307999-13308018 None:intergenic 45.0%
CGTCTCAACATTGCCAAAGA+AGG + chr4.3:13306511-13306530 MS.gene065976:CDS 45.0%
CTTACCATGGTTGATCTTCC+AGG + chr4.3:13306847-13306866 MS.gene065976:CDS 45.0%
GAAGAGTCTCCGTCTATTTC+TGG + chr4.3:13308251-13308270 MS.gene065976:CDS 45.0%
GAGACGCCTTAGATTCTCAA+GGG - chr4.3:13306497-13306516 None:intergenic 45.0%
GATACCTGGAAGATCAACCA+TGG - chr4.3:13306854-13306873 None:intergenic 45.0%
GATGACGTTAACATCGGTCT+AGG + chr4.3:13307114-13307133 MS.gene065976:CDS 45.0%
GTTGACCATGAACAGGTACA+CGG - chr4.3:13306879-13306898 None:intergenic 45.0%
TCCTGAAGGATTGTTGGAGA+AGG + chr4.3:13307083-13307102 MS.gene065976:CDS 45.0%
TGAGACGCCTTAGATTCTCA+AGG - chr4.3:13306498-13306517 None:intergenic 45.0%
TGGTGTTCCTGATCTTACCA+TGG + chr4.3:13306834-13306853 MS.gene065976:CDS 45.0%
TTACCCTCATGTGCTAAGTC+AGG + chr4.3:13308052-13308071 MS.gene065976:CDS 45.0%
TTGACCATGAACAGGTACAC+GGG - chr4.3:13306878-13306897 None:intergenic 45.0%
TTGGAAGAGGCCAAATGGAT+TGG + chr4.3:13307615-13307634 MS.gene065976:CDS 45.0%
! CATGTGAAGCTTGAAGGTGT+TGG + chr4.3:13308002-13308021 MS.gene065976:CDS 45.0%
! GATCTTTTGTCTCCAAGTGG+TGG + chr4.3:13308209-13308228 MS.gene065976:CDS 45.0%
! TTTTGTCTCCAAGTGGTGGT+GGG + chr4.3:13308213-13308232 MS.gene065976:CDS 45.0%
!! ACTGGAAGATTGTTCTGAGG+AGG + chr4.3:13308105-13308124 MS.gene065976:CDS 45.0%
!! AGATTGCTGGTTCTGCCAAA+GGG + chr4.3:13306773-13306792 MS.gene065976:CDS 45.0%
!! AGGAGTGTTGGAAATCCCTT+TGG - chr4.3:13306791-13306810 None:intergenic 45.0%
!! CTCCGTCTATTTCTGGAAAG+AGG + chr4.3:13308258-13308277 MS.gene065976:CDS 45.0%
!! TGATTTCGAAGAGTACCCTG+AGG + chr4.3:13307473-13307492 MS.gene065976:CDS 45.0%
!! TTGTCAGGTTGACCATGAAC+AGG - chr4.3:13306886-13306905 None:intergenic 45.0%
ATCACCCGTGTACCTGTTCA+TGG + chr4.3:13306871-13306890 MS.gene065976:CDS 50.0%
CAATGCATGTGCTTGTCCTC+AGG - chr4.3:13307492-13307511 None:intergenic 50.0%
GTCTGTGTGCGAAACCGAAT+TGG + chr4.3:13307141-13307160 MS.gene065976:CDS 50.0%
TCGACGCCCTTGAGAATCTA+AGG + chr4.3:13306488-13306507 MS.gene065976:CDS 50.0%
TGGACAAGGAATCTGCACGA+GGG + chr4.3:13306624-13306643 MS.gene065976:CDS 50.0%
! CTTTTGTCTCCAAGTGGTGG+TGG + chr4.3:13308212-13308231 MS.gene065976:CDS 50.0%
! GAGGTTGAGCTTGCTCAACA+TGG - chr4.3:13306414-13306433 None:intergenic 50.0%
!! AGGTGTTGGAAGAGGCCAAA+TGG + chr4.3:13307610-13307629 MS.gene065976:CDS 50.0%
!! GAGATTGCTGGTTCTGCCAA+AGG + chr4.3:13306772-13306791 MS.gene065976:CDS 50.0%
ACAGGTACACGGGTGATACC+TGG - chr4.3:13306868-13306887 None:intergenic 55.0%
ATCAGCTTGCCACGTGGACA+AGG + chr4.3:13306610-13306629 MS.gene065976:CDS 55.0%
CTCTCAATCCCACCACCACT+TGG - chr4.3:13308224-13308243 None:intergenic 55.0%
GTGCAGATTCCTTGTCCACG+TGG - chr4.3:13306622-13306641 None:intergenic 55.0%
GTGGACAAGGAATCTGCACG+AGG + chr4.3:13306623-13306642 MS.gene065976:CDS 55.0%
!! CAAGTGGTGGTGGGATTGAG+AGG + chr4.3:13308222-13308241 MS.gene065976:CDS 55.0%
!! TTCTCAAGGGCGTCGAGAAC+TGG - chr4.3:13306484-13306503 None:intergenic 55.0%
CAGGTTTCCACTCGCAGAGC+AGG + chr4.3:13307801-13307820 MS.gene065976:CDS 60.0%
GCTGGTATCAGCTTGCCACG+TGG + chr4.3:13306604-13306623 MS.gene065976:CDS 60.0%
TTTCTCGCCTGCTCTGCGAG+TGG - chr4.3:13307811-13307830 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 13306331 13308367 13306331 ID=MS.gene065976
chr4.3 mRNA 13306331 13308367 13306331 ID=MS.gene065976.t1;Parent=MS.gene065976
chr4.3 exon 13306331 13308367 13306331 ID=MS.gene065976.t1.exon1;Parent=MS.gene065976.t1
chr4.3 CDS 13306331 13308367 13306331 ID=cds.MS.gene065976.t1;Parent=MS.gene065976.t1
Gene Sequence

>MS.gene065976

ATGGTTGTGACTTCAAAGAGAACCACTACAAAAGCTTCTGTTAGTGCTAACCAACGAGATGAAACATCACTTTCATTAGTCCATGTTGAGCAAGCTCAACCTCTTTCTGTTGTTGCACCAATTGTTTCATCCTACAATGAAAAAATACGTCCAGTTCTCGACGCCCTTGAGAATCTAAGGCGTCTCAACATTGCCAAAGAAGGAATTCAGCTTCCAACAATTGTTGTTGTTGGAGATCAATCTTCAGGAAAGTCCAGTGTCCTTGAATCTTTAGCTGGTATCAGCTTGCCACGTGGACAAGGAATCTGCACGAGGGTACCCTTAATTATGCGCCTTCAGAATCATCCACTTTCAAAGCCAGAACTCTTGTTAGAATTCAATGGCAAGACAGTTTTCACTGATGAAAAAGATGTTTCTGATGCAATAAACATAGCTACTGAAGAGATTGCTGGTTCTGCCAAAGGGATTTCCAACACTCCTTTGACTTTGATTGTCAAGAAAAATGGTGTTCCTGATCTTACCATGGTTGATCTTCCAGGTATCACCCGTGTACCTGTTCATGGTCAACCTGACAATATCTATGATCAGATAAAAGATATTATTATGGAGTATATAACTCCTGAAGAAAGTATTATCTTGAATGTCCTTTCCGCCACTGTTGATTTTACTACTTGTGAGTCTATCAGGATGTCACAAATGGTTGATAAAACTGGTTTGAGGACACTAGCTGTTGTGACAAAAGCAGATAAATCTCCTGAAGGATTGTTGGAGAAGGTTACTGCTGATGACGTTAACATCGGTCTAGGTTATGTCTGTGTGCGAAACCGAATTGGAGATGAGACCTATGAAGATGCTAGATTGGAAGAAGAAAAGCTGTTCGAGTCTCACTCACTTCTTTCTAGGATTGACAAATCCATTGTTGGTATTCCTGTTTTAGCTCAGAAGCTTGTTCAGGTTCAAGCTATGATTATCTCAAAAACTTTGCCAGAGATCATCAAGAAGATTAATGAGAAGCTTGCTTCTAATGCAAATGAGTTAGAGAATTTACCTGCTAATTTATCTTCTGTTGCTGATGCTATGACAGCTTTTATGCATATTCTTGGTTTGTCTAGAGATTCTCTCAAAAAGATTCTTCTTACAGGTGATTTCGAAGAGTACCCTGAGGACAAGCACATGCATTGCACTGCTCGATTAGTTGAGATGCTTAACTTATATGCAAGCGATCTTGAAAACTGTGCTGAAAGTGATGCGAAAAAGAATTTTCTTATGGAAGAGATTAAGGTGTTGGAAGAGGCCAAATGGATTGGTCTACCAAATTTCATGCCAAGAACTGCATTTTTGACAATACTGCAGAGAAAAGTTAAGGGCATTTCTTATATGCCAGTTAACTTTGTTGATAATGTTTGGAACTATCTTGAATCAGTTGTGATTTCTATTTTGAATCATCATTCTTCAAATTATTATCAGCTTCAGGTTTCCACTCGCAGAGCAGGCGAGAAATTGATTGCTAAGAAGAAGAAAAGTTCAGTCCAACATGTTATGGAAGCTGTTGAGATGGAAAAACTGACAGATTACACTTGTAATCCTGAGTATTTGCTCGAGTATAATAGGCTGATAGCTCAACAAGAAGCGTTTGTGAAAGAAGTTCTAAGCAATGAAACAAAACCAACACATGTGAAGCTTGAAGGTGTTGGTGAAATTGATGTTGTTAATCTGAGGAATTACCCTCATGTGCTAAGTCAGGCTTTTGATTTGAAGGCTAGGATGATTGCTTACTGGAAGATTGTTCTGAGGAGGCTCATTGACAGCATTGCTCTGCACTTGATGCTAAGCATCAATGAACTGATTAACAATGATTTGCAGAAGGAGATTTGTAATGATCTTTTGTCTCCAAGTGGTGGTGGGATTGAGAGGCTGCTTGAAGAGTCTCCGTCTATTTCTGGAAAGAGGGAGAAGCTAAGCCGAAGTGTCAAAGTTCTTAGAGAAAGTAAGGAGACTGTTGCAAGAATTATGGACAGGATTGGAATCTATGATTAG
Protein sequence

>MS.gene065976.t1

MVVTSKRTTTKASVSANQRDETSLSLVHVEQAQPLSVVAPIVSSYNEKIRPVLDALENLRRLNIAKEGIQLPTIVVVGDQSSGKSSVLESLAGISLPRGQGICTRVPLIMRLQNHPLSKPELLLEFNGKTVFTDEKDVSDAINIATEEIAGSAKGISNTPLTLIVKKNGVPDLTMVDLPGITRVPVHGQPDNIYDQIKDIIMEYITPEESIILNVLSATVDFTTCESIRMSQMVDKTGLRTLAVVTKADKSPEGLLEKVTADDVNIGLGYVCVRNRIGDETYEDARLEEEKLFESHSLLSRIDKSIVGIPVLAQKLVQVQAMIISKTLPEIIKKINEKLASNANELENLPANLSSVADAMTAFMHILGLSRDSLKKILLTGDFEEYPEDKHMHCTARLVEMLNLYASDLENCAESDAKKNFLMEEIKVLEEAKWIGLPNFMPRTAFLTILQRKVKGISYMPVNFVDNVWNYLESVVISILNHHSSNYYQLQVSTRRAGEKLIAKKKKSSVQHVMEAVEMEKLTDYTCNPEYLLEYNRLIAQQEAFVKEVLSNETKPTHVKLEGVGEIDVVNLRNYPHVLSQAFDLKARMIAYWKIVLRRLIDSIALHLMLSINELINNDLQKEICNDLLSPSGGGIERLLEESPSISGKREKLSRSVKVLRESKETVARIMDRIGIYD