AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066006

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066006.t1 MTR_8g088190 95.497 866 13 4 1 841 1 865 0.0 1652
MS.gene066006.t1 MTR_3g048270 24.218 863 507 28 18 841 23 777 1.62e-18 91.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066006.t1 AT1G29350 43.483 913 359 41 2 841 3 831 2.77e-179 537
MS.gene066006.t1 AT1G29370 43.231 916 357 41 2 841 3 831 5.05e-179 536
MS.gene066006.t1 AT1G29370 43.775 747 301 33 139 841 20 691 6.18e-150 457
MS.gene066006.t1 AT4G18150 33.891 897 384 40 6 834 3 758 2.94e-91 304

Find 262 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACAAAATGCCACTGTTTC+TGG 0.131454 4.3:-12973501 MS.gene066006:CDS
TGAGAATGGTAGAAATTATT+AGG 0.188167 4.3:+12969458 None:intergenic
TTGTCAAGATGCAGGCTTTA+AGG 0.189462 4.3:-12970900 MS.gene066006:intron
TGCAGAAATCCCGACTATTA+TGG 0.197232 4.3:-12973305 MS.gene066006:intron
TTTGTATAATACTCAGAATT+TGG 0.210938 4.3:+12974413 None:intergenic
AGTCTGAGCTTTGGAAGTTT+TGG 0.226818 4.3:-12973528 MS.gene066006:CDS
ACTGCTTAGGTGGTTGGCTT+TGG 0.229453 4.3:+12969375 None:intergenic
CTGCTTAGGTGGTTGGCTTT+GGG 0.240326 4.3:+12969376 None:intergenic
GCAGGCATTGCTTGTGTAAC+AGG 0.247275 4.3:+12972276 None:intergenic
TATGATTGTTATCCTTATAC+TGG 0.256360 4.3:+12970470 None:intergenic
CCAGGCAAAGATTGTTGATT+AGG 0.276204 4.3:+12970847 None:intergenic
ATATTCCTTTCAGCTGAAAA+TGG 0.278424 4.3:-12973681 MS.gene066006:intron
GATAATCCAGGAACTGCTTC+TGG 0.289516 4.3:-12974254 MS.gene066006:CDS
GGAAGTTCCACCAGCATTCC+TGG 0.294224 4.3:-12970565 MS.gene066006:CDS
ATACCATCTGGATATGGTTT+TGG 0.299580 4.3:-12970586 MS.gene066006:CDS
GTAACAGGGAAGGGTGAGTA+TGG 0.301650 4.3:+12972291 None:intergenic
ACATACCATTGTTACTGAAT+TGG 0.307160 4.3:+12975682 None:intergenic
GCTACTGATGTTTCAGCAAT+TGG 0.311438 4.3:-12973423 MS.gene066006:CDS
CTCCTCCAAGTTACTTTGTA+AGG 0.312134 4.3:+12973455 None:intergenic
AATGCTGCTGCGATGGCTGC+TGG 0.323633 4.3:+12969498 None:intergenic
CCTGGTGCTGGTGTTGCCAC+TGG 0.327217 4.3:-12970829 MS.gene066006:CDS
CCCGTGTTTAAGAAAGAAAA+TGG 0.342626 4.3:-12975386 MS.gene066006:CDS
CGTGATAAACTCCCAGTATA+AGG 0.343410 4.3:-12970482 MS.gene066006:CDS
CCTAGGGATGCTTCCCTTGC+CGG 0.343729 4.3:-12969401 MS.gene066006:CDS
GCAGAAATCCCGACTATTAT+GGG 0.344135 4.3:-12973304 MS.gene066006:intron
GACTAAAACACTCTGGAGTA+TGG 0.347028 4.3:+12973547 None:intergenic
TCATCACTCTGTTGAACATT+GGG 0.349769 4.3:+12974482 None:intergenic
AGTAACTTGGAGGAGACTTC+TGG 0.354552 4.3:-12973447 MS.gene066006:CDS
GAAAATGGAACGCCTGCTTA+TGG 0.359441 4.3:-12975371 MS.gene066006:CDS
TGAACATTGGGTTCAATAGA+AGG 0.361624 4.3:+12974533 None:intergenic
GTATGGAGTTGTAGGTGATT+TGG 0.365100 4.3:+12973564 None:intergenic
TGTCAAGATGCAGGCTTTAA+GGG 0.371015 4.3:-12970899 MS.gene066006:intron
CCACTGTGGACAGAAGATTT+AGG 0.371116 4.3:+12974632 None:intergenic
AGCAACATGGCTTCTTCAAT+TGG 0.373493 4.3:-12972246 MS.gene066006:CDS
TCATCACTCTGTTGAACATT+GGG 0.374072 4.3:+12974521 None:intergenic
TCATATCCAGAAGCAGTTCC+TGG 0.381408 4.3:+12974248 None:intergenic
AGGTGGTTGGCTTTGGGAGC+CGG 0.383810 4.3:+12969382 None:intergenic
GTGGCATAGATCTCGTTATC+AGG 0.387328 4.3:+12976122 None:intergenic
TATCCAATGGTTGTACTAGT+AGG 0.392226 4.3:+12970511 None:intergenic
TTCTTTGCCCAATGCTCTGT+TGG 0.399402 4.3:-12972346 MS.gene066006:CDS
ACCGGCGGCGAGTCGGAAAA+TGG 0.402820 4.3:-12976177 MS.gene066006:CDS
GGCAATATATCCCACATAAC+TGG 0.405437 4.3:-12973254 MS.gene066006:CDS
TAAACACGGGTTTACTCTGC+TGG 0.406086 4.3:+12975399 None:intergenic
GTTGGGACTGCTTAGGTGGT+TGG 0.411643 4.3:+12969369 None:intergenic
TAAAGCCTCTGGTTGTGAAA+TGG 0.415654 4.3:+12973202 None:intergenic
GAATGAGGATATGCAACTTC+TGG 0.418587 4.3:+12973092 None:intergenic
ATATGCAACTTCTGGTTGTT+GGG 0.418891 4.3:+12973100 None:intergenic
TGGAGAAGGGAGATAGGTTC+TGG 0.424665 4.3:+12973051 None:intergenic
GGTTCAGACTTTAAAGCCTC+TGG 0.425019 4.3:+12973191 None:intergenic
CAATGCTCTGTTGGCTTCAA+CGG 0.425294 4.3:-12972337 MS.gene066006:CDS
ATCATCGTTGTCGGGTATAC+CGG 0.425443 4.3:-12976195 MS.gene066006:CDS
GTTGGCTTTGGGAGCCGGCA+AGG 0.425841 4.3:+12969387 None:intergenic
GCTGCTATACCATCTGGATA+TGG 0.428437 4.3:-12970592 MS.gene066006:CDS
TGTGCATCCGTACTCACAGC+TGG 0.430757 4.3:-12970782 MS.gene066006:CDS
GTTGTTGTTGATTCTGTCCC+TGG 0.432205 4.3:+12969543 None:intergenic
AGACGACTAACAGCTTCGTT+AGG 0.434553 4.3:+12976077 None:intergenic
ACTCTGGAGTATGGAGTTGT+AGG 0.436906 4.3:+12973556 None:intergenic
CCGAGTCGCGGCGGCGGTGG+TGG 0.440407 4.3:-12975759 MS.gene066006:CDS
TATAATACTCAGAATTTGGA+TGG 0.442532 4.3:+12974417 None:intergenic
GAAGCAGATCCAACATCAAC+AGG 0.444671 4.3:+12974302 None:intergenic
TCACCTTCGTTGTCATCAAA+AGG 0.445731 4.3:+12974179 None:intergenic
CAGTCTGCTGCTATACCATC+TGG 0.448979 4.3:-12970598 MS.gene066006:CDS
TGATGGACAATAATGTAAAC+AGG 0.450601 4.3:-12975319 MS.gene066006:CDS
GTAAGGATGAGCGGCAAAGT+AGG 0.453242 4.3:-12976236 None:intergenic
CTGGAAGCTGTAATACGTGC+TGG 0.453247 4.3:+12969562 None:intergenic
TGAACATTGGGGTCAATAGA+AGG 0.454561 4.3:+12974494 None:intergenic
GAGAATGGTAGAAATTATTA+GGG 0.455029 4.3:+12969459 None:intergenic
AACAATCCTATGTGGGTTCA+AGG 0.455407 4.3:-12969614 MS.gene066006:CDS
ATTATATCTGATGATAATCC+AGG 0.457309 4.3:-12974266 MS.gene066006:CDS
ATACCGAGTCGCGGCGGCGG+TGG 0.457632 4.3:-12975762 MS.gene066006:CDS
ATCCAATGGTTGTACTAGTA+GGG 0.460599 4.3:+12970512 None:intergenic
CTACTGATGTTTCAGCAATT+GGG 0.460752 4.3:-12973422 MS.gene066006:CDS
TTGGCTTTGGGAGCCGGCAA+GGG 0.466160 4.3:+12969388 None:intergenic
AATTGTTGTTGGGACTGCTT+AGG 0.468035 4.3:+12969362 None:intergenic
AACATGGCTTCTTCAATTGG+TGG 0.468306 4.3:-12972243 MS.gene066006:CDS
GTCGCGGCGGCGGTGGTGGT+AGG 0.468646 4.3:-12975755 MS.gene066006:CDS
GGCGGCGGTGGTGGTAGGAC+TGG 0.471364 4.3:-12975750 MS.gene066006:CDS
TTCATCACTCTGTTGAACAT+TGG 0.471449 4.3:+12974520 None:intergenic
TTATACAAATACATCTAACT+TGG 0.471805 4.3:-12974399 MS.gene066006:CDS
TCATATGATTTCACTTCAGC+AGG 0.476681 4.3:-12970452 MS.gene066006:intron
CATGCCATCGGCATTTCAGC+AGG 0.476730 4.3:-12970707 MS.gene066006:CDS
CAGCACGTATTACAGCTTCC+AGG 0.479728 4.3:-12969561 MS.gene066006:CDS
CAACAATCTTTGCCTGGTGC+TGG 0.482125 4.3:-12970841 MS.gene066006:CDS
CGCCACCACGGCCAGCATAT+CGG 0.484251 4.3:+12975721 None:intergenic
TTCATCACTCTGTTGAACAT+TGG 0.484635 4.3:+12974481 None:intergenic
TAAGGATGAGCGGCAAAGTA+GGG 0.485905 4.3:-12976235 None:intergenic
CAAAAGGATGTCGATGAGAC+TGG 0.486306 4.3:+12974195 None:intergenic
GAAGCACCGCTGCCAGAGAT+TGG 0.486453 4.3:+12970659 None:intergenic
TTACACTGGAGAAGGGAGAT+AGG 0.487511 4.3:+12973045 None:intergenic
ACATCAGAATTGTCCTCCTC+TGG 0.487648 4.3:+12973594 None:intergenic
AGATTTAGGCACGGATGCCT+TGG 0.488437 4.3:+12974646 None:intergenic
GTTGAAGCCAACAGAGCATT+GGG 0.490742 4.3:+12972339 None:intergenic
CCTATGTGGGTTCAAGGGCC+TGG 0.491914 4.3:-12969608 MS.gene066006:CDS
CCACCACCGCCGCCGCGACT+CGG 0.494335 4.3:+12975759 None:intergenic
CGTTGAAGCCAACAGAGCAT+TGG 0.495525 4.3:+12972338 None:intergenic
GATATGCAACTTCTGGTTGT+TGG 0.498257 4.3:+12973099 None:intergenic
GAGCCCAGGTCAAGTGTCAA+TGG 0.500187 4.3:-12974699 MS.gene066006:CDS
TAGCACCTACCACCAATCTC+TGG 0.500268 4.3:-12970671 MS.gene066006:CDS
ACATAACTGGTGTGGATGCT+AGG 0.500709 4.3:-12973241 MS.gene066006:CDS
TCAGCCATTGACACTTGACC+TGG 0.501383 4.3:+12974695 None:intergenic
TGCTCATCCCCATAATAGTC+GGG 0.501814 4.3:+12973296 None:intergenic
AGGTACATCATCATCGTTGT+CGG 0.502101 4.3:-12976204 MS.gene066006:CDS
TCTGCATGGTCGGCGAGCCC+AGG 0.502549 4.3:-12974713 MS.gene066006:CDS
TAGCATCCACACCAGTTATG+TGG 0.502709 4.3:+12973243 None:intergenic
GGCACGGATGCCTTGGCCTG+TGG 0.503151 4.3:+12974653 None:intergenic
AATGCCACTGTTTCTGGAGC+CGG 0.504252 4.3:-12973495 MS.gene066006:CDS
TAACTTATCATATTAGCAAA+AGG 0.504719 4.3:+12970757 None:intergenic
TAGGCCCTTACAAAGTAACT+TGG 0.504725 4.3:-12973460 MS.gene066006:CDS
CCGGCAAGGGAAGCATCCCT+AGG 0.505021 4.3:+12969401 None:intergenic
TAAACAGGCAGCCACCATCT+TGG 0.509603 4.3:-12975304 MS.gene066006:CDS
AAGTTGTGATGCGAGGCTGC+TGG 0.511504 4.3:+12974584 None:intergenic
TGAACCCTCTGAAACTGCTC+AGG 0.513104 4.3:-12973174 MS.gene066006:CDS
TTCTGTTGCAGTGATTCTGT+TGG 0.513872 4.3:-12974782 MS.gene066006:intron
CGATATGCTGGCCGTGGTGG+CGG 0.514178 4.3:-12975720 MS.gene066006:CDS
TGTGAGCTTGCTTGTGAATG+AGG 0.515023 4.3:+12973077 None:intergenic
GTGGAGGAGCCTCCATAAGC+AGG 0.516664 4.3:+12975359 None:intergenic
GGTGCAAAATGCTGCTGCGA+TGG 0.517018 4.3:+12969491 None:intergenic
GATGGGTAGGCCACAGGCCA+AGG 0.517499 4.3:-12974663 MS.gene066006:CDS
ACTGACATTGTTCGAGAACC+AGG 0.517878 4.3:+12969590 None:intergenic
ATAGTTTCCACCAGGAATGC+TGG 0.518703 4.3:+12970555 None:intergenic
GAGAAATCCCACTCTGAGAA+TGG 0.519187 4.3:+12969444 None:intergenic
ATCTCCCTTCTCCAGTGTAA+TGG 0.521345 4.3:-12973042 MS.gene066006:intron
TTACTGAAGCAAATAATGAA+TGG 0.524202 4.3:-12974556 MS.gene066006:CDS
GACGACTAACAGCTTCGTTA+GGG 0.524337 4.3:+12976078 None:intergenic
ATGCCACTGTTTCTGGAGCC+GGG 0.524558 4.3:-12973494 MS.gene066006:CDS
GAGCCGGGACACATTCATCT+AGG 0.527788 4.3:-12973479 MS.gene066006:CDS
CATTACCATTACACTGGAGA+AGG 0.529209 4.3:+12973037 None:intergenic
GCCCTTACAAAGTAACTTGG+AGG 0.530352 4.3:-12973457 MS.gene066006:CDS
ACTGGTAGTGACCGATATGC+TGG 0.533784 4.3:-12975732 MS.gene066006:CDS
ATTTCTACCATTCTCAGAGT+GGG 0.534749 4.3:-12969451 MS.gene066006:CDS
CGGGTATACCGGCGGCGAGT+CGG 0.537690 4.3:-12976184 MS.gene066006:CDS
TAATTACTCCCACGTGACCT+TGG 0.540089 4.3:+12975789 None:intergenic
AACATCAGCAGCAGAACCCT+AGG 0.541212 4.3:-12969418 MS.gene066006:CDS
GGTACATCATCATCGTTGTC+GGG 0.541357 4.3:-12976203 MS.gene066006:CDS
TATGCTGGCCGTGGTGGCGG+TGG 0.541966 4.3:-12975717 MS.gene066006:CDS
CCAGTGGCAACACCAGCACC+AGG 0.542760 4.3:+12970829 None:intergenic
GTAAACTCATGCGAAGATGA+AGG 0.543874 4.3:+12973134 None:intergenic
TCCTCCAAGTTACTTTGTAA+GGG 0.545463 4.3:+12973456 None:intergenic
ATGCTCATCCCCATAATAGT+CGG 0.545903 4.3:+12973295 None:intergenic
AAAGGTCCCAGCTGTGAGTA+CGG 0.546189 4.3:+12970775 None:intergenic
TGGCTTTGTCGAAATGCACC+TGG 0.547698 4.3:+12969518 None:intergenic
CAGCTACAAATTCATTTCCG+TGG 0.549215 4.3:+12974351 None:intergenic
CCTAATCAACAATCTTTGCC+TGG 0.549819 4.3:-12970847 MS.gene066006:CDS
TGTCAAGATGGGTAGGCCAC+AGG 0.551390 4.3:-12974669 MS.gene066006:CDS
AACTTGCACCACCGCCACCA+CGG 0.554060 4.3:+12975709 None:intergenic
TTCTTCCATTTCACAACCAG+AGG 0.555873 4.3:-12973207 MS.gene066006:CDS
AACCACATACTTGGCATCTG+AGG 0.563576 4.3:+12973398 None:intergenic
AAAGATAAATACCTCCAAGA+TGG 0.563649 4.3:+12975290 None:intergenic
GTCTTATGCATCTAGTGTGA+TGG 0.563807 4.3:-12975336 MS.gene066006:CDS
CATTGCTTGTGTAACAGGGA+AGG 0.569151 4.3:+12972281 None:intergenic
ACAGGCAGCCACCATCTTGG+AGG 0.569197 4.3:-12975301 MS.gene066006:intron
TGTTGTTGGGACTGCTTAGG+TGG 0.569521 4.3:+12969365 None:intergenic
TGTTGATTAGGTTGAGCTTG+AGG 0.571227 4.3:+12970859 None:intergenic
TGGAAGCTGTAATACGTGCT+GGG 0.572788 4.3:+12969563 None:intergenic
GAGTATGGGATATCCTCCCT+TGG 0.575189 4.3:+12972306 None:intergenic
AATATACCGAGTCGCGGCGG+CGG 0.576843 4.3:-12975765 MS.gene066006:CDS
AATGGCTGACATTGTCAAGA+TGG 0.577715 4.3:-12974681 MS.gene066006:CDS
CTACCACCAATCTCTGGCAG+CGG 0.578357 4.3:-12970665 MS.gene066006:CDS
AGCACGTATTACAGCTTCCA+GGG 0.578501 4.3:-12969560 MS.gene066006:CDS
ATGTTCAACAGAGTGATGAA+TGG 0.579550 4.3:-12974478 MS.gene066006:CDS
ATGTTCAACAGAGTGATGAA+TGG 0.579550 4.3:-12974517 MS.gene066006:CDS
GTATAGCTCTGAGGCATGAA+CGG 0.580543 4.3:+12970733 None:intergenic
GGGCCTAGATGAATGTGTCC+CGG 0.582233 4.3:+12973476 None:intergenic
CAGGCATTGCTTGTGTAACA+GGG 0.583522 4.3:+12972277 None:intergenic
GTACTAGTAGGGGCAGCTGA+TGG 0.583533 4.3:+12970523 None:intergenic
ATGATTGTTATCCTTATACT+GGG 0.584516 4.3:+12970471 None:intergenic
CCAGGCCCTTGAACCCACAT+AGG 0.584672 4.3:+12969608 None:intergenic
GATGAGAATCCTGTTGATGT+TGG 0.584695 4.3:-12974311 MS.gene066006:CDS
GCCCCTACTAGTACAACCAT+TGG 0.585988 4.3:-12970514 MS.gene066006:CDS
AGCATCCACACCAGTTATGT+GGG 0.586141 4.3:+12973244 None:intergenic
GGGGCAGCTGATGGATTCAA+AGG 0.586575 4.3:+12970532 None:intergenic
ATGGCTGACATTGTCAAGAT+GGG 0.587015 4.3:-12974680 MS.gene066006:CDS
CTGTCTTCATCATCACATCA+TGG 0.587021 4.3:-12974746 MS.gene066006:CDS
ACAATCCTATGTGGGTTCAA+GGG 0.590132 4.3:-12969613 MS.gene066006:CDS
TATCACGTCATCATATCCAA+TGG 0.592769 4.3:+12970498 None:intergenic
CATGTTACAGTCTTTGAGAG+TGG 0.592942 4.3:+12976103 None:intergenic
TAACAGGGAAGGGTGAGTAT+GGG 0.593054 4.3:+12972292 None:intergenic
GGATGCACAGCAAGGTGTTG+AGG 0.593434 4.3:+12970796 None:intergenic
GATAAATACCTCCAAGATGG+TGG 0.593978 4.3:+12975293 None:intergenic
TATACAAATACATCTAACTT+GGG 0.595181 4.3:-12974398 MS.gene066006:CDS
CATATTGCTACTACCTCAGA+TGG 0.595267 4.3:-12973275 MS.gene066006:CDS
ATTGCTTGTGTAACAGGGAA+GGG 0.596414 4.3:+12972282 None:intergenic
GTGCATCCGTACTCACAGCT+GGG 0.596507 4.3:-12970781 MS.gene066006:CDS
AGAATGAGAACAATCCTATG+TGG 0.598777 4.3:-12969622 MS.gene066006:CDS
CATCAACAACAACTGTTGGT+GGG 0.601667 4.3:+12974441 None:intergenic
AAGCTCAGACTAAAACACTC+TGG 0.601824 4.3:+12973540 None:intergenic
CTAAATCTTCTGTCCACAGT+GGG 0.603435 4.3:-12974631 MS.gene066006:CDS
GTTGGTGGGTGCTCAACAGA+AGG 0.604734 4.3:+12974455 None:intergenic
TTCAAAGGATAGTTTCCACC+AGG 0.605161 4.3:+12970547 None:intergenic
AATGGAACGCCTGCTTATGG+AGG 0.606529 4.3:-12975368 MS.gene066006:CDS
GCAAGGTGTTGAGGAAGTGA+AGG 0.606894 4.3:+12970805 None:intergenic
CCTAAATCTTCTGTCCACAG+TGG 0.609597 4.3:-12974632 MS.gene066006:CDS
ATAATACTCAGAATTTGGAT+GGG 0.610849 4.3:+12974418 None:intergenic
ACAACAGAACCAAGGTCACG+TGG 0.611217 4.3:-12975798 MS.gene066006:CDS
TATATCCCACATAACTGGTG+TGG 0.611883 4.3:-12973249 MS.gene066006:CDS
GAGCTATACCTACATGCCAT+CGG 0.612525 4.3:-12970719 MS.gene066006:CDS
GCACCGCTGCCAGAGATTGG+TGG 0.613306 4.3:+12970662 None:intergenic
AGTGACCGATATGCTGGCCG+TGG 0.613894 4.3:-12975726 MS.gene066006:CDS
ATATGATGATAATTTGTACA+AGG 0.614124 4.3:-12974231 MS.gene066006:CDS
AGCAGTCCCAACAACAATTG+TGG 0.614795 4.3:-12969358 MS.gene066006:CDS
CGGCAAGGGAAGCATCCCTA+GGG 0.614858 4.3:+12969402 None:intergenic
AATTTACACACAGATGAGCA+AGG 0.615714 4.3:-12973624 MS.gene066006:CDS
CCAATGCATTACCATTACAC+TGG 0.615927 4.3:+12973031 None:intergenic
TGAGCAAGGGACAGAACCAG+AGG 0.616906 4.3:-12973610 MS.gene066006:CDS
CTTCCAAAACCATATCCAGA+TGG 0.617034 4.3:+12970583 None:intergenic
CTGACATTGTCAAGATGGGT+AGG 0.617049 4.3:-12974676 MS.gene066006:CDS
TCGAACCAATTCAGTAACAA+TGG 0.617541 4.3:-12975687 MS.gene066006:intron
TCCAATGGTTGTACTAGTAG+GGG 0.620354 4.3:+12970513 None:intergenic
GCATCAACAACAACTGTTGG+TGG 0.620968 4.3:+12974440 None:intergenic
AACATTCAAGTTGTGATGCG+AGG 0.621627 4.3:+12974577 None:intergenic
CAGCCATTGACACTTGACCT+GGG 0.622423 4.3:+12974696 None:intergenic
TGGGCATCAACAACAACTGT+TGG 0.624347 4.3:+12974437 None:intergenic
TTGAGGAAGTGAAGGTCCAG+TGG 0.626322 4.3:+12970813 None:intergenic
AAGGATGAGCGGCAAAGTAG+GGG 0.626473 4.3:-12976234 None:intergenic
GAATGAGAACAATCCTATGT+GGG 0.626818 4.3:-12969621 MS.gene066006:CDS
CACACTAGATGCATAAGACG+TGG 0.629917 4.3:+12975340 None:intergenic
TGGATTGCAATCTGCATGGT+CGG 0.630287 4.3:-12974723 MS.gene066006:CDS
AAAGCCTGCTGAAATGCCGA+TGG 0.631956 4.3:+12970703 None:intergenic
CGCTGCCAGAGATTGGTGGT+AGG 0.632641 4.3:+12970666 None:intergenic
TACCTCTGACATGTTTATCG+TGG 0.632657 4.3:+12972218 None:intergenic
GTGGGATATATTGCCATCTG+AGG 0.632746 4.3:+12973262 None:intergenic
AATTTCTACCATTCTCAGAG+TGG 0.633896 4.3:-12969452 MS.gene066006:CDS
GATTCTGTTGGTGTCCATGA+CGG 0.634224 4.3:-12974770 MS.gene066006:CDS
CTGAAATGCCGATGGCATGT+AGG 0.634576 4.3:+12970711 None:intergenic
GACCGATATGCTGGCCGTGG+TGG 0.634825 4.3:-12975723 MS.gene066006:CDS
AGCTGAAGACAGTTCTGTTG+AGG 0.637818 4.3:-12974333 MS.gene066006:CDS
AGGATGAGCGGCAAAGTAGG+GGG 0.638263 4.3:-12976233 MS.gene066006:CDS
TGAAAATGGTGTTTCATCAG+TGG 0.638285 4.3:-12973667 MS.gene066006:CDS
AACGGTTCAAACACCAAGGG+AGG 0.641331 4.3:-12972319 MS.gene066006:CDS
GCAAGGGACAGAACCAGAGG+AGG 0.642696 4.3:-12973607 MS.gene066006:CDS
ATGATGAAGACAGTCCGTCA+TGG 0.643081 4.3:+12974756 None:intergenic
GATGGTATAGCAGCAGACTG+AGG 0.645781 4.3:+12970601 None:intergenic
CAGAATCAACAACAACAACC+AGG 0.645981 4.3:-12969536 MS.gene066006:CDS
ACATCAGCAGCAGAACCCTA+GGG 0.646794 4.3:-12969417 MS.gene066006:CDS
CAGAAATCCCGACTATTATG+GGG 0.649539 4.3:-12973303 MS.gene066006:CDS
ATTTACACACAGATGAGCAA+GGG 0.649851 4.3:-12973623 MS.gene066006:CDS
ATCGTTGTCGGGTATACCGG+CGG 0.650301 4.3:-12976192 MS.gene066006:CDS
GTGAGTACGGATGCACAGCA+AGG 0.655238 4.3:+12970788 None:intergenic
CACTCTCAAAGACTGTAACA+TGG 0.656952 4.3:-12976102 MS.gene066006:CDS
GTTTCCACCAGGAATGCTGG+TGG 0.657460 4.3:+12970558 None:intergenic
ATTACCATTACACTGGAGAA+GGG 0.657757 4.3:+12973038 None:intergenic
CTTCAACGGTTCAAACACCA+AGG 0.659295 4.3:-12972323 MS.gene066006:CDS
CATCACTCTGTTGAACATTG+GGG 0.665234 4.3:+12974483 None:intergenic
TATAATATACCGAGTCGCGG+CGG 0.665825 4.3:-12975768 MS.gene066006:CDS
AATAGACAACTGCAGAACCA+CGG 0.668240 4.3:-12974368 MS.gene066006:CDS
GGGATCGGAGTAAGGATGAG+CGG 0.672525 4.3:-12976245 None:intergenic
GCCTGCAAAATATAGCAACA+TGG 0.677043 4.3:-12972259 MS.gene066006:CDS
CATTCTGATGATTCCCACTG+TGG 0.681276 4.3:+12974618 None:intergenic
TTCCACGATAAACATGTCAG+AGG 0.686512 4.3:-12972220 MS.gene066006:intron
AGTTCCACCAGCATTCCTGG+TGG 0.687385 4.3:-12970562 MS.gene066006:CDS
AATTATAATATACCGAGTCG+CGG 0.693817 4.3:-12975771 MS.gene066006:CDS
GTGGACAGAAGATTTAGGCA+CGG 0.697837 4.3:+12974637 None:intergenic
TTCAACGGTTCAAACACCAA+GGG 0.698105 4.3:-12972322 MS.gene066006:CDS
ATGGTGGATTGCAATCTGCA+TGG 0.698767 4.3:-12974727 MS.gene066006:CDS
TGTCCCGGCTCCAGAAACAG+TGG 0.704706 4.3:+12973491 None:intergenic
TCTTCATCATCACATCATGG+TGG 0.706000 4.3:-12974743 MS.gene066006:CDS
ACTAGATGCATAAGACGTGG+AGG 0.713779 4.3:+12975343 None:intergenic
GGCATGTAGGTATAGCTCTG+AGG 0.714504 4.3:+12970724 None:intergenic
AAGCTGTAATACGTGCTGGG+AGG 0.724449 4.3:+12969566 None:intergenic
CAACAGAACCAAGGTCACGT+GGG 0.726867 4.3:-12975797 MS.gene066006:CDS
GTAAGGATACAACAGAACCA+AGG 0.748600 4.3:-12975806 MS.gene066006:CDS
AAATTATTAGGGTACCCGTG+CGG 0.792199 4.3:+12969470 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr4.3 gene 12969343 12976252 12969343 ID=MS.gene066006
chr4.3 mRNA 12969343 12976252 12969343 ID=MS.gene066006.t1;Parent=MS.gene066006
chr4.3 exon 12976088 12976252 12976088 ID=MS.gene066006.t1.exon1;Parent=MS.gene066006.t1
chr4.3 CDS 12976088 12976252 12976088 ID=cds.MS.gene066006.t1;Parent=MS.gene066006.t1
chr4.3 exon 12975688 12975829 12975688 ID=MS.gene066006.t1.exon2;Parent=MS.gene066006.t1
chr4.3 CDS 12975688 12975829 12975688 ID=cds.MS.gene066006.t1;Parent=MS.gene066006.t1
chr4.3 exon 12975302 12975431 12975302 ID=MS.gene066006.t1.exon3;Parent=MS.gene066006.t1
chr4.3 CDS 12975302 12975431 12975302 ID=cds.MS.gene066006.t1;Parent=MS.gene066006.t1
chr4.3 exon 12974183 12974793 12974183 ID=MS.gene066006.t1.exon4;Parent=MS.gene066006.t1
chr4.3 CDS 12974183 12974793 12974183 ID=cds.MS.gene066006.t1;Parent=MS.gene066006.t1
chr4.3 exon 12973408 12973690 12973408 ID=MS.gene066006.t1.exon5;Parent=MS.gene066006.t1
chr4.3 CDS 12973408 12973690 12973408 ID=cds.MS.gene066006.t1;Parent=MS.gene066006.t1
chr4.3 exon 12973043 12973322 12973043 ID=MS.gene066006.t1.exon6;Parent=MS.gene066006.t1
chr4.3 CDS 12973043 12973322 12973043 ID=cds.MS.gene066006.t1;Parent=MS.gene066006.t1
chr4.3 exon 12972221 12972379 12972221 ID=MS.gene066006.t1.exon7;Parent=MS.gene066006.t1
chr4.3 CDS 12972221 12972379 12972221 ID=cds.MS.gene066006.t1;Parent=MS.gene066006.t1
chr4.3 exon 12970453 12970908 12970453 ID=MS.gene066006.t1.exon8;Parent=MS.gene066006.t1
chr4.3 CDS 12970453 12970908 12970453 ID=cds.MS.gene066006.t1;Parent=MS.gene066006.t1
chr4.3 exon 12969343 12969642 12969343 ID=MS.gene066006.t1.exon9;Parent=MS.gene066006.t1
chr4.3 CDS 12969343 12969642 12969343 ID=cds.MS.gene066006.t1;Parent=MS.gene066006.t1
Gene Sequence

>MS.gene066006

ATGAGCGGCAAAGTAGGGGGACAGAAAGGTACATCATCATCGTTGTCGGGTATACCGGCGGCGAGTCGGAAAATGGTACAAAGCTTGAAAGAGATAGTTTCGAATATACCTGATAACGAGATCTATGCCACTCTCAAAGACTGTAACATGGACCCTAACGAAGCTGTTAGTCGTCTTCTTTCTCAAGGTTTCTCTCTCTCCCTTTCTCTCTCATCACTAACTAGTCAGGGTTTTTCAATTTCTTTAATTAATCTAACAATTTACTCTTTTTCTTCTTGTAGATCCTTTTCATGAGGTTAAGAGCAAACGAGAAAAGAAAAAAGAGGTTTTTTTTACTTGTTCTTGTTCTTTTAACTTTTTATTATTTTTATTATTATGTATTGTGAAGAGTTAGTTACATGTTTTTTTTTTGTATTTGTTTAGGGTAAGGATACAACAGAACCAAGGTCACGTGGGAGTAATTATAATATACCGAGTCGCGGCGGCGGTGGTGGTAGGACTGGTAGTGACCGATATGCTGGCCGTGGTGGCGGTGGTGCAAGTTCGAACCAATTCAGTAACAATGGTATGTATTTCGAATCTGGATGATGTTTGTTTCTATTTTTGGTATATTTCTTAATTTGTGTTTTTTTTACTGTATAAGTTGATAACTATATCCACTTTAGTGTAAAAAAGTCTTACACTTTCAGAGATTTCTAAATTTTATGTTGATGTTTTAAGAAATAATGTCATTCATGACCAAGAAACGATTTGATGACTTTGTAAATGTTGTTGCTATTGCTATTATTATTTTAATTTTCGCCGTTGTTTTTGGAAATCAGACTATGGTGTCCAGCAGAGTAAACCCGTGTTTAAGAAAGAAAATGGAACGCCTGCTTATGGAGGCTCCTCCACGTCTTATGCATCTAGTGTGATGGACAATAATGTAAACAGGCAGCCACCATCTTGGAGGTATTTATCTTTTTCTTCATATTTATACTAAAATAACTGTACATGGTTGGAGTGGATAAAAACTAAATTCTTGTGGTTATGATGTGTTTGGAATTAAGTTCTTGATGCTATAAAGTGAAACTAAATCTAGCCATTTCTATATGGCTTGAAAATCCGATGCTATTGAAAAGGGTTCCTCCAATTTATCCCAAAAGCAAGTTGGCTGTCAAAGTATCCTCTTTTTGAAAACTTAATTTCAATATAGATTCACTTTTATTCTTATCTAAAACAGCTGGTTCACTCTGTGCTGATTAATTCTGTGGATTAGTTATGGTTCCAATGTATCTCGCTATGGCATCAGTTTTATAAATCAATAAATGCCTATTTTGGTCCCCTGAAGATAAATGAGATGTAAAAAATGATCACATCCCTCTTCCATATGTAGAAGTATTATAAAGTAATTGAAGACTAAACAGTATTGATATGAAGGCCAAGATGGCTGTTTCACCATTATTCAATTCTGTTGCAGTGATTCTGTTGGTGTCCATGACGGACTGTCTTCATCATCACATCATGGTGGATTGCAATCTGCATGGTCGGCGAGCCCAGGTCAAGTGTCAATGGCTGACATTGTCAAGATGGGTAGGCCACAGGCCAAGGCATCCGTGCCTAAATCTTCTGTCCACAGTGGGAATCATCAGAATGCTTTTTCAACTCCAGCAGCCTCGCATCACAACTTGAATGTTACTGAAGCAAATAATGAATGGCCTTCTATTGAACCCAATGTTCAACAGAGTGATGAATGGCCTTCTATTGACCCCAATGTTCAACAGAGTGATGAATGGCCTTCTGTTGAGCACCCACCAACAGTTGTTGTTGATGCCCATCCAAATTCTGAGTATTATACAAATACATCTAACTTGGGTGAAGCTAATAGACAACTGCAGAACCACGGAAATGAATTTGTAGCTGAAGACAGTTCTGTTGAGGATGAGAATCCTGTTGATGTTGGATCTGCTTCTATACCTGCTAAAATTATATCTGATGATAATCCAGGAACTGCTTCTGGATATGATGATAATTTGTACAAGGACATAAATTCTTACCAGTCTCATCGACATCCTTTTGATGACAACGAAGGTGAATCAGATACTTCTTTTTATTGCATTGTTGTCCATGTTTTCCATAAAATTTGCTTTAAATGATCATGTAATTGAAGTGATGGTACCATTATGTGAATTAAATTCGCTTTGAAATTCATCAGTTTGTACATTATTATTTTATGGTTGTTCCATGTGTAGTTGGTTTTAAAAATAAACATGTTAGATTTTTGCCAGAAATATCTAAGTTATAGAAATAACATAAGGAAACATGCATCCATTTGTTGAGTCAATCTGGTGATATTATTATTTCTATTGGTTTATTTCTGTTACTAATTATCTAACTCCGTATGTGTGAATGGTTTAAAAGGCTTGCATTGCTAATATAGCCATGTACCTGTGTGTATGCTTTCTAATTGTGTAAAAAATATTACATTAAATTGTAATGCTATTATTTGATATTTGATTATTCGACATGTGCCTTACATACATGTGGTCTCTGAGTCTTAATTTCTGGAAATATTCCTTTCAGCTGAAAATGGTGTTTCATCAGTGGCTACAAACTTAGAGCAGCTAAATTTACACACAGATGAGCAAGGGACAGAACCAGAGGAGGACAATTCTGATGTACTTATTCCAAATCACCTACAACTCCATACTCCAGAGTGTTTTAGTCTGAGCTTTGGAAGTTTTGGACCAAAACAAAATGCCACTGTTTCTGGAGCCGGGACACATTCATCTAGGCCCTTACAAAGTAACTTGGAGGAGACTTCTGGAGCTACTGATGTTTCAGCAATTGGGTCCTCAGATGCCAAGTATGTGGTTATAAAATAGGATGCTTTACTGCATATTCTTGTCTCATTCATAGTTACTTAAGCACATGTGTAATTTTTCTTGCAGAAATCCCGACTATTATGGGGATGAGCATATTGCTACTACCTCAGATGGCAATATATCCCACATAACTGGTGTGGATGCTAGGACTTATGAACATTCTTCCATTTCACAACCAGAGGCTTTAAAGTCTGAACCCTCTGAAACTGCTCAGGAAAATCAATATTCATTTCCTTCATCTTCGCATGAGTTTACATATGAAAATGCCCAACAACCAGAAGTTGCATATCCTCATTCACAAGCAAGCTCACAGATCCAGAACCTATCTCCCTTCTCCAGTGTAATGGTAATGCATTGGTCTGTTAGATTTTGGATGTGCAACAATATATATTTTAAAGCTGGTGTCTAGTTTGGCTCTTTAATTAACTAAAATTGGTTCATAAATGATATAACTAGAGTTTGGAGATCCTGTTTGTTAAAAACTAGGTCATCGTTGGTGCTATATTACTTACCCATTCTTAAGAGAGTAAAATGGCGTTTATTCTCTGCAAAAAATCTTTTTGATAGTAGAAAACTGGCTTACTGTAAGAAATATAATTTTCAAGACTGTCTTGTTTTGTATCTTTAATTAATTATAATCAGTTCTGGAATGAACCAATGTGAAACCGCGTAATAAGCTAGGGGATGGTTATGTGATATATATGATTGGATTGAGAATTTGTTAAAATTTCTTTCTCCGGGATGTTTCATTCTTTTGGATTGGGTGATGTGGGTTTTCTACTAATTAAAATTTTGCTGCTCAGTTGCTTTACTAATTAAACCTGTATACAAACCCTTTCCTTAATATAATACATAATACTTTTGTCCCATTACATTATATTATGGTGCAGAGATCACAACTGTCGTGTGGTAATAACATAGGATATTACTTTAGTTTCTTCTTTTATCTGCAAATGTTGATATTATCTGTTGATATTACTATCTACATCTAACTTATATTGCTTTATAGGCATATACAAATTCTTTGCCCAATGCTCTGTTGGCTTCAACGGTTCAAACACCAAGGGAGGATATCCCATACTCACCCTTCCCTGTTACACAAGCAATGCCTGCAAAATATAGCAACATGGCTTCTTCAATTGGTGGTTCCACGATAAACATGTCAGAGGTATACTTCTTGCTGACTTATATAAGCTCATCTATTAACAAGCTATTGAAAGAGGCTGAGTTATAAATAGTTTTAATATAACGGCTTGCAGTATTTGTCTATTCTTACCCAAATAATGTTATGGAGTTCATGTGTACATTAAAGGGCTGCAGTTACACGCCGATAATTCTGGTTAACAATTGCTAAAAACTAATGACAACCACATCCGCTAAGGGAATGCAAATTTCTCCCTAGATTTTGTGTAGCTCAGTGTTGGTGTTCTATGTGTGTTACCTATGATGCACAAATACGATACGGGGATGCGTCGTTTATTGGAGAATATAAGTTATGATATGTTCTGGATACATGTATAAAAAATATGAATAATTTATTAACTGATATATTTTTTTAAAAAGATCAAATGTTTGTCTATGTTTATGTAGTTTCATATATAAAATATCAAATATCACATATAAACTAAATCAGTTATAATATTATAATTAAAAATCATTTATAAATATAATTAATTAATAAGATCTGTGCTCAAAATGTGTTATAATATTTTGATTTATTACTAAATGAGATAAAATATCAAAATCACATATAAACTAAATCAGTTATAATATTATAATTAAAAATCATTTATAAATATAACTAATTAATAAGATCTGTGTTCACAATGTGTTATAATAGTTTGATTTATTACTAAATGAGAAATCATATATACAATCAAAATAATTAAAATAGAATTACCAGGATAGCACCTCCAGGGGAAATGAAACTTTTTTTTAAGGGGAAATGAAACTCGATATGAGGAAATGACGAACGAAAGCTGAAGGATAGAGTGGTCTAGGCAAAAAGTTTCTCTTGTTCGGTTTAACCCAACTATATTTTTGCAGTACTATGTTGTTTAATTGCCTGATAAATGCTAGTCATCTACGAGGGACAAGTCTTACATTGTTCTATGCCTATAAAATAGTAACCAATTTTGGAAGTATCAATATAGAGTCTGTGTCTGTGTAAAATCATATTATCTTTTATCCCAAATTTTGTTACGGGTATTCGAAATAGCAAAGTGAATCAAAGGAATGTTCTTAGAGGGGAGGTTATGGATACATGTGTACCAACATTGTTTGTGTATATGTAGAATTATATCAGTTTTAACCCCCTTTTTTTAAGGGTACGCCAAACTAACTCTAATAAAAATTGTCCAGTAAAGATGATTATAATTGACATAGTTTAGTTTTAAGTTGGTGTCTTGATTTTATTGGTTCAGGGAGAGGGAGAACACATGAATTCAGTCTTTTGATGATTTTTTTTTGTCAAGATGCAGGCTTTAAGGGCAAACAGCATTTCGACGCCTCAAGCTCAACCTAATCAACAATCTTTGCCTGGTGCTGGTGTTGCCACTGGACCTTCACTTCCTCAACACCTTGCTGTGCATCCGTACTCACAGCTGGGACCTTTTGCTAATATGATAAGTTATCCGTTCATGCCTCAGAGCTATACCTACATGCCATCGGCATTTCAGCAGGCTTTTGCTGGAAATAGCACCTACCACCAATCTCTGGCAGCGGTGCTTCCACAATATAAAAATAGCATTTCTGTCAGCAGCTTGCCTCAGTCTGCTGCTATACCATCTGGATATGGTTTTGGAAGTTCCACCAGCATTCCTGGTGGAAACTATCCTTTGAATCCATCAGCTGCCCCTACTAGTACAACCATTGGATATGATGACGTGATAAACTCCCAGTATAAGGATAACAATCATATGATTTCACTTCAGCAGGTAGATTTTTTTTTTCCTTCCAAAATGCTTGATTAAAGTTGATTGTCTTCCCTGTTAATCTTTCTTCTCTAAGTAGTTGAGTCAGTTTGGCATCACTAGTTCCATGCAGCCATTGTAATGTAAACAGACGGGTTGACATGTGGACCATGTGCTTCATTTAGTTGTCTTTATGTCATGTTCTTTTTGGCAAGGAGATGCAAGGCCAGTGGTGGTTAGGAAAAGAAAATAAATACAGTAGAAACTCTTTAATTTAATACTCGGTAAATTAATAAACTCTCTAAAATAATAAATTTGTCCGGTCCCGACTTGGGCCAATGTAAAAAATTGAAAAACTCGATAAAATAATAAGATAATAATTTTTCGGGAGATCCCTTTATAATTTTCGGTCCCAAGAAAATCATAAATTAATAATTCATAGAAACTGAAAAAAAATATATTAAGAATACATTGAACACATTTGTTCCTCTTGTTTACATTTACTGTACTTCAAAAGTCATTATTGACTTCCAATGCATATGAACACAGTAGTTACTAATTTACGTTGAGTCAGTCACAAGGTTCGCTGCAACGTAATTCTAAGAGGCATAGACTAATTTGCCTTTTTGTTTGGTATGTATAGTATAATTACTTAAAAACTCTTTAAATTAATAATTATTAATTTATCGATAAATTAATAACTCTCTAAATTAATAAATTCCTTCGGTCCTGACATTATTAATTTATAGAGTTTTAACTGTAATAATCATATGACTTGTTATATTTTGGCTTATTATATGGAAGGAATTTACTGATTGTGATTTCTTATGTCAGAATGAGAACAATCCTATGTGGGTTCAAGGGCCTGGTTCTCGAACAATGTCAGTTCCTCCCAGCACGTATTACAGCTTCCAGGGACAGAATCAACAACAACAACCAGGTGCATTTCGACAAAGCCAGCAGCCATCGCAGCAGCATTTTGCACCGCACGGGTACCCTAATAATTTCTACCATTCTCAGAGTGGGATTTCTCTAGAACATCAGCAGCAGAACCCTAGGGATGCTTCCCTTGCCGGCTCCCAAAGCCAACCACCTAAGCAGTCCCAACAACAATTGTGGCAAAACAGCTACTAA
Protein sequence

>MS.gene066006.t1

MSGKVGGQKGTSSSLSGIPAASRKMVQSLKEIVSNIPDNEIYATLKDCNMDPNEAGKDTTEPRSRGSNYNIPSRGGGGGRTGSDRYAGRGGGGASSNQFSNNDYGVQQSKPVFKKENGTPAYGGSSTSYASSVMDNNVNRQPPSWSDSVGVHDGLSSSSHHGGLQSAWSASPGQVSMADIVKMGRPQAKASVPKSSVHSGNHQNAFSTPAASHHNLNVTEANNEWPSIEPNVQQSDEWPSIDPNVQQSDEWPSVEHPPTVVVDAHPNSEYYTNTSNLGEANRQLQNHGNEFVAEDSSVEDENPVDVGSASIPAKIISDDNPGTASGYDDNLYKDINSYQSHRHPFDDNEAENGVSSVATNLEQLNLHTDEQGTEPEEDNSDVLIPNHLQLHTPECFSLSFGSFGPKQNATVSGAGTHSSRPLQSNLEETSGATDVSAIGSSDAKNPDYYGDEHIATTSDGNISHITGVDARTYEHSSISQPEALKSEPSETAQENQYSFPSSSHEFTYENAQQPEVAYPHSQASSQIQNLSPFSSVMAYTNSLPNALLASTVQTPREDIPYSPFPVTQAMPAKYSNMASSIGGSTINMSEALRANSISTPQAQPNQQSLPGAGVATGPSLPQHLAVHPYSQLGPFANMISYPFMPQSYTYMPSAFQQAFAGNSTYHQSLAAVLPQYKNSISVSSLPQSAAIPSGYGFGSSTSIPGGNYPLNPSAAPTSTTIGYDDVINSQYKDNNHMISLQQNENNPMWVQGPGSRTMSVPPSTYYSFQGQNQQQQPGAFRQSQQPSQQHFAPHGYPNNFYHSQSGISLEHQQQNPRDASLAGSQSQPPKQSQQQLWQNSY