Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066074.t1 | XP_013470234.1 | 98.8 | 82 | 1 | 0 | 3 | 84 | 150 | 231 | 1.80E-36 | 161.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066074.t1 | A0A072VR59 | 98.8 | 82 | 1 | 0 | 3 | 84 | 150 | 231 | 1.3e-36 | 161.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059439 | MS.gene066074 | 0.80028 | 1.55E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAAGCAAAATCAAAGCTTT+TGG | 0.288180 | 1.4:+85422540 | None:intergenic |
| CAACACAACACCACGAGTTT+CGG | 0.311167 | 1.4:+85422638 | None:intergenic |
| AACACAACACCACGAGTTTC+GGG | 0.322665 | 1.4:+85422639 | None:intergenic |
| AAAGGTTATGTTGGTCTTAG+TGG | 0.349558 | 1.4:-85422606 | MS.gene066074:CDS |
| TATATTTCACTTGGTCTAGC+TGG | 0.381529 | 1.4:-85422414 | None:intergenic |
| GAAACTCGTGGTGTTGTGTT+GGG | 0.407607 | 1.4:-85422636 | MS.gene066074:CDS |
| AGTTCGTTGTTCTGTTTAAC+CGG | 0.420863 | 1.4:+85422459 | None:intergenic |
| AAAAGAAAGGAAATTGCTGC+TGG | 0.480135 | 1.4:+85422507 | None:intergenic |
| CGAAACTCGTGGTGTTGTGT+TGG | 0.484732 | 1.4:-85422637 | MS.gene066074:CDS |
| AACCATTCGATACATGAAAC+CGG | 0.515497 | 1.4:-85422478 | MS.gene066074:CDS |
| AACCGGTTTCATGTATCGAA+TGG | 0.520822 | 1.4:+85422476 | None:intergenic |
| GTTAAACAGAACAACGAACT+TGG | 0.531720 | 1.4:-85422456 | MS.gene066074:CDS |
| AAACTCGTGGTGTTGTGTTG+GGG | 0.568454 | 1.4:-85422635 | MS.gene066074:CDS |
| CACAACACCACGAGTTTCGG+GGG | 0.581648 | 1.4:+85422641 | None:intergenic |
| AAAGAAAGGAAATTGCTGCT+GGG | 0.629241 | 1.4:+85422508 | None:intergenic |
| ACACAACACCACGAGTTTCG+GGG | 0.651587 | 1.4:+85422640 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGTTTTTGTATATTTCACT+TGG | - | chr1.4:85422638-85422657 | MS.gene066074:CDS | 20.0% |
| ! | AATGGTTCGAATAAAAAGAA+AGG | + | chr1.4:85422570-85422589 | None:intergenic | 25.0% |
| !! | ATAAGCAAAATCAAAGCTTT+TGG | + | chr1.4:85422524-85422543 | None:intergenic | 25.0% |
| !! | CTTTGATTTTGCTTATTGCT+TGG | - | chr1.4:85422528-85422547 | MS.gene066074:CDS | 30.0% |
| !! | GGGATTTTAAAAGGTTATGT+TGG | - | chr1.4:85422446-85422465 | MS.gene066074:CDS | 30.0% |
| AAAAGAAAGGAAATTGCTGC+TGG | + | chr1.4:85422557-85422576 | None:intergenic | 35.0% | |
| AAAGAAAGGAAATTGCTGCT+GGG | + | chr1.4:85422556-85422575 | None:intergenic | 35.0% | |
| AACCATTCGATACATGAAAC+CGG | - | chr1.4:85422583-85422602 | MS.gene066074:CDS | 35.0% | |
| AGTTCGTTGTTCTGTTTAAC+CGG | + | chr1.4:85422605-85422624 | None:intergenic | 35.0% | |
| GTTAAACAGAACAACGAACT+TGG | - | chr1.4:85422605-85422624 | MS.gene066074:CDS | 35.0% | |
| ! | GTTGTGTTGGGGATTTTAAA+AGG | - | chr1.4:85422437-85422456 | MS.gene066074:CDS | 35.0% |
| !! | AAAGGTTATGTTGGTCTTAG+TGG | - | chr1.4:85422455-85422474 | MS.gene066074:CDS | 35.0% |
| ! | AACCGGTTTCATGTATCGAA+TGG | + | chr1.4:85422588-85422607 | None:intergenic | 40.0% |
| AACACAACACCACGAGTTTC+GGG | + | chr1.4:85422425-85422444 | None:intergenic | 45.0% | |
| CAACACAACACCACGAGTTT+CGG | + | chr1.4:85422426-85422445 | None:intergenic | 45.0% | |
| !! | AAACTCGTGGTGTTGTGTTG+GGG | - | chr1.4:85422426-85422445 | MS.gene066074:CDS | 45.0% |
| !! | GAAACTCGTGGTGTTGTGTT+GGG | - | chr1.4:85422425-85422444 | MS.gene066074:CDS | 45.0% |
| ACACAACACCACGAGTTTCG+GGG | + | chr1.4:85422424-85422443 | None:intergenic | 50.0% | |
| !! | CGAAACTCGTGGTGTTGTGT+TGG | - | chr1.4:85422424-85422443 | MS.gene066074:CDS | 50.0% |
| CACAACACCACGAGTTTCGG+GGG | + | chr1.4:85422423-85422442 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 85422415 | 85422668 | 85422415 | ID=MS.gene066074 |
| chr1.4 | mRNA | 85422415 | 85422668 | 85422415 | ID=MS.gene066074.t1;Parent=MS.gene066074 |
| chr1.4 | exon | 85422415 | 85422668 | 85422415 | ID=MS.gene066074.t1.exon1;Parent=MS.gene066074.t1 |
| chr1.4 | CDS | 85422415 | 85422668 | 85422415 | ID=cds.MS.gene066074.t1;Parent=MS.gene066074.t1 |
| Gene Sequence |
| Protein sequence |
