AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066185

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066185.t1 MTR_2g059540 82.326 215 28 2 7 220 1 206 8.12e-107 306
MS.gene066185.t1 MTR_2g096740 43.421 152 74 3 20 171 46 185 8.73e-33 120
MS.gene066185.t1 MTR_2g096740 43.421 152 74 3 20 171 61 200 1.14e-32 120
MS.gene066185.t1 MTR_4g109980 54.331 127 58 0 35 161 39 165 2.16e-30 114
MS.gene066185.t1 MTR_3g091820 82.456 57 10 0 35 91 42 98 4.13e-29 110
MS.gene066185.t1 MTR_4g022370 42.446 139 67 2 8 146 54 179 5.00e-29 111
MS.gene066185.t1 MTR_4g063780 44.531 128 58 2 35 161 74 189 9.02e-29 110
MS.gene066185.t1 MTR_8g015840 76.667 60 14 0 36 95 20 79 1.81e-28 107
MS.gene066185.t1 MTR_8g068210 61.842 76 29 0 31 106 27 102 2.06e-28 109
MS.gene066185.t1 MTR_4g088580 53.571 112 43 2 6 115 32 136 5.97e-28 109
MS.gene066185.t1 MTR_8g079060 62.667 75 28 0 36 110 29 103 6.27e-28 105
MS.gene066185.t1 MTR_7g059400 54.348 92 27 1 37 113 77 168 8.24e-28 108
MS.gene066185.t1 MTR_8g479350 63.158 76 21 1 36 104 68 143 8.65e-28 108
MS.gene066185.t1 MTR_7g024670 73.438 64 17 0 35 98 76 139 1.20e-27 108
MS.gene066185.t1 MTR_8g027295 80.000 55 11 0 36 90 24 78 2.70e-27 105
MS.gene066185.t1 MTR_3g090430 67.164 67 22 0 31 97 46 112 3.24e-27 106
MS.gene066185.t1 MTR_1g077600 72.414 58 16 0 35 92 42 99 4.94e-27 104
MS.gene066185.t1 MTR_1g077600 72.414 58 16 0 35 92 42 99 6.18e-27 104
MS.gene066185.t1 MTR_2g014060 74.138 58 15 0 30 87 19 76 6.90e-27 105
MS.gene066185.t1 MTR_2g093220 62.667 75 21 1 16 90 27 94 7.91e-27 104
MS.gene066185.t1 MTR_7g010950 65.714 70 23 1 36 105 135 203 8.18e-27 107
MS.gene066185.t1 MTR_8g044220 56.164 73 32 0 19 91 101 173 1.11e-26 106
MS.gene066185.t1 MTR_2g013370 57.692 78 32 1 15 92 25 101 1.70e-26 103
MS.gene066185.t1 MTR_6g012450 58.442 77 27 1 20 91 115 191 1.95e-26 106
MS.gene066185.t1 MTR_4g461080 62.921 89 28 2 35 118 28 116 2.02e-26 103
MS.gene066185.t1 MTR_3g077750 56.977 86 34 1 1 86 29 111 9.91e-26 102
MS.gene066185.t1 MTR_2g014170 85.714 49 7 0 35 83 44 92 1.03e-25 102
MS.gene066185.t1 MTR_7g086780 56.164 73 32 0 17 89 80 152 1.10e-25 103
MS.gene066185.t1 MTR_5g031440 78.431 51 11 0 36 86 43 93 1.42e-25 102
MS.gene066185.t1 MTR_3g435480 62.319 69 26 0 36 104 136 204 1.96e-25 103
MS.gene066185.t1 MTR_2g016030 58.108 74 28 1 36 106 47 120 2.78e-25 97.1
MS.gene066185.t1 MTR_4g082060 66.102 59 20 0 36 94 122 180 1.07e-24 101
MS.gene066185.t1 MTR_1g056810 64.516 62 22 0 29 90 5 66 2.30e-24 97.8
MS.gene066185.t1 MTR_6g027450 67.273 55 18 0 36 90 89 143 4.52e-24 97.8
MS.gene066185.t1 MTR_4g089095 73.684 57 14 1 36 92 50 105 5.91e-24 97.1
MS.gene066185.t1 MTR_4g089095 73.684 57 14 1 36 92 16 71 6.67e-24 96.3
MS.gene066185.t1 MTR_5g041380 63.492 63 23 0 36 98 127 189 1.50e-22 94.4
MS.gene066185.t1 MTR_5g041530 52.778 72 34 0 18 89 122 193 1.59e-22 94.4
MS.gene066185.t1 MTR_5g041400 63.492 63 23 0 36 98 119 181 1.72e-22 94.0
MS.gene066185.t1 MTR_6g027460 66.667 54 18 0 36 89 96 149 1.74e-22 94.0
MS.gene066185.t1 MTR_5g041420 48.148 81 42 0 18 98 120 200 2.08e-22 93.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066185.t1 AT5G60850 71.429 91 23 2 18 108 34 121 3.09e-39 137
MS.gene066185.t1 AT1G51700 68.421 76 24 0 23 98 20 95 2.18e-32 116
MS.gene066185.t1 AT5G60200 62.338 77 25 1 33 109 52 124 1.91e-30 113
MS.gene066185.t1 AT3G45610 65.333 75 21 1 35 109 39 108 3.56e-30 112
MS.gene066185.t1 AT5G65590 75.758 66 16 0 36 101 41 106 4.97e-30 113
MS.gene066185.t1 AT5G66940 74.242 66 16 1 36 100 32 97 6.19e-30 111
MS.gene066185.t1 AT3G55370 70.149 67 20 0 35 101 75 141 1.88e-29 112
MS.gene066185.t1 AT3G55370 70.149 67 20 0 35 101 75 141 2.07e-29 112
MS.gene066185.t1 AT3G55370 70.149 67 20 0 35 101 120 186 4.16e-29 112
MS.gene066185.t1 AT3G21270 88.462 52 6 0 36 87 29 80 6.06e-29 107
MS.gene066185.t1 AT1G07640 81.034 58 10 1 36 92 21 78 6.63e-29 109
MS.gene066185.t1 AT1G07640 81.034 58 10 1 36 92 77 134 1.05e-28 110
MS.gene066185.t1 AT1G07640 81.034 58 10 1 36 92 85 142 1.36e-28 110
MS.gene066185.t1 AT3G61850 74.194 62 16 0 36 97 62 123 2.52e-28 108
MS.gene066185.t1 AT3G61850 74.194 62 16 0 36 97 62 123 2.52e-28 108
MS.gene066185.t1 AT3G61850 74.194 62 16 0 36 97 74 135 2.68e-28 108
MS.gene066185.t1 AT3G61850 74.194 62 16 0 36 97 62 123 3.94e-28 108
MS.gene066185.t1 AT3G61850 74.194 62 16 0 36 97 74 135 4.36e-28 108
MS.gene066185.t1 AT4G38000 76.786 56 13 0 35 90 40 95 6.56e-28 106
MS.gene066185.t1 AT3G50410 62.963 81 26 2 17 93 8 88 8.04e-28 106
MS.gene066185.t1 AT2G37590 56.410 78 34 0 15 92 68 145 1.27e-27 107
MS.gene066185.t1 AT1G28310 78.947 57 12 0 36 92 27 83 2.64e-27 106
MS.gene066185.t1 AT2G28810 81.132 53 10 0 35 87 76 128 2.70e-27 106
MS.gene066185.t1 AT1G28310 78.947 57 12 0 36 92 51 107 3.91e-27 106
MS.gene066185.t1 AT2G28810 81.132 53 10 0 35 87 93 145 4.35e-27 106
MS.gene066185.t1 AT3G52440 60.274 73 29 0 33 105 22 94 7.06e-27 103
MS.gene066185.t1 AT3G52440 64.706 68 24 0 38 105 46 113 1.10e-26 103
MS.gene066185.t1 AT4G21050 66.197 71 23 1 38 107 26 96 1.68e-26 101
MS.gene066185.t1 AT4G24060 76.786 56 13 0 35 90 52 107 1.68e-26 104
MS.gene066185.t1 AT2G28510 70.492 61 18 0 35 95 47 107 4.07e-26 102
MS.gene066185.t1 AT5G02460 75.000 56 14 0 35 90 94 149 1.01e-25 103
MS.gene066185.t1 AT1G21340 70.492 61 18 0 35 95 37 97 1.10e-25 100
MS.gene066185.t1 AT1G64620 81.633 49 9 0 35 83 48 96 1.38e-25 102
MS.gene066185.t1 AT4G00940 81.132 53 10 0 38 90 70 122 1.42e-25 101
MS.gene066185.t1 AT4G00940 81.132 53 10 0 38 90 70 122 1.42e-25 101
MS.gene066185.t1 AT4G00940 81.132 53 10 0 38 90 70 122 1.42e-25 101
MS.gene066185.t1 AT3G47500 50.943 106 36 3 17 118 91 184 1.80e-25 103
MS.gene066185.t1 AT1G29160 58.571 70 29 0 36 105 62 131 2.04e-25 98.2
MS.gene066185.t1 AT5G39660 74.074 54 14 0 36 89 138 191 4.35e-25 102
MS.gene066185.t1 AT5G39660 74.074 54 14 0 36 89 138 191 4.35e-25 102
MS.gene066185.t1 AT5G39660 74.074 54 14 0 36 89 138 191 4.35e-25 102
MS.gene066185.t1 AT2G34140 72.222 54 15 0 36 89 58 111 5.93e-25 96.7
MS.gene066185.t1 AT2G46590 58.586 99 36 2 36 131 80 176 6.19e-25 100
MS.gene066185.t1 AT2G46590 58.586 99 36 2 36 131 68 164 6.70e-25 100
MS.gene066185.t1 AT1G47655 71.930 57 14 1 29 83 20 76 2.01e-24 96.3
MS.gene066185.t1 AT5G62430 47.115 104 54 1 36 138 54 157 2.20e-24 98.2
MS.gene066185.t1 AT4G21040 60.000 70 28 0 38 107 27 96 9.50e-24 95.1
MS.gene066185.t1 AT1G69570 70.370 54 16 0 36 89 132 185 1.70e-23 97.4
MS.gene066185.t1 AT1G26790 70.370 54 16 0 36 89 111 164 2.28e-23 96.7
MS.gene066185.t1 AT5G62940 78.000 50 11 0 37 86 74 123 4.58e-23 95.9
MS.gene066185.t1 AT4G21080 58.824 68 28 0 38 105 27 94 3.89e-22 91.3
MS.gene066185.t1 AT4G21030 58.333 60 25 0 38 97 23 82 1.76e-15 72.4

Find 53 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAATGATGTTGTTTCTTA+TGG 0.089712 2.2:+28905766 MS.gene066185:CDS
CAGCAGTTATCAAATGAATT+TGG 0.217916 2.2:+28905847 MS.gene066185:CDS
CCAATGATGTTGTTTCTTAT+GGG 0.265532 2.2:+28905767 MS.gene066185:CDS
TCTTTGATTTCTTTGATTTA+CGG 0.270159 2.2:-28905570 None:intergenic
CTTTGATTTCTTTGATTTAC+GGG 0.312389 2.2:-28905569 None:intergenic
GCACCGTTGATGAGTCTTAC+TGG 0.380552 2.2:+28905896 MS.gene066185:CDS
ATGAATTTGGATCGTTGGAT+TGG 0.382150 2.2:+28905860 MS.gene066185:CDS
GTAAGAACTGCCGTCGCTAC+TGG 0.393302 2.2:+28905503 MS.gene066185:CDS
GATGTTGTTTCTTATGGGCT+TGG 0.395727 2.2:+28905772 MS.gene066185:CDS
ACAGTGAAGGTTCAACCGTT+AGG 0.410245 2.2:+28905680 MS.gene066185:CDS
ATCAAATGAATTTGGATCGT+TGG 0.421631 2.2:+28905855 MS.gene066185:CDS
TGAATTTGGATCGTTGGATT+GGG 0.437910 2.2:+28905861 MS.gene066185:CDS
GATGATGATAATTCTGGTTA+TGG 0.445814 2.2:-28905387 None:intergenic
ATGTTGTTTCTTATGGGCTT+GGG 0.446424 2.2:+28905773 MS.gene066185:CDS
CAGTGAAGGTTCAACCGTTA+GGG 0.455669 2.2:+28905681 MS.gene066185:CDS
TGCCGTCGCTACTGGACTAT+AGG 0.462115 2.2:+28905511 MS.gene066185:CDS
CCCATAAGAAACAACATCAT+TGG 0.464285 2.2:-28905767 None:intergenic
TGGCGTGCTTGCTGCTGAAA+CGG 0.467885 2.2:-28905614 None:intergenic
CACGCCAACACCACCTCCAC+TGG 0.474815 2.2:+28905630 MS.gene066185:CDS
CTTACTGGACACACTCTCAT+TGG 0.475567 2.2:+28905911 MS.gene066185:CDS
TTATCAGCTTCCAGTGGAGG+TGG 0.483911 2.2:-28905640 None:intergenic
AATGCAAGAAATTCATTCGA+TGG 0.486084 2.2:+28905315 None:intergenic
GAAATTCATTCGATGGGAGG+TGG 0.516720 2.2:+28905322 MS.gene066185:CDS
ATGATAATTCTGGTTATGGT+GGG 0.521689 2.2:-28905383 None:intergenic
GCTTCCAGTGGAGGTGGTGT+TGG 0.521828 2.2:-28905634 None:intergenic
GATGATAATTCTGGTTATGG+TGG 0.523805 2.2:-28905384 None:intergenic
GGATCGTTGGATTGGGAAGG+TGG 0.526946 2.2:+28905868 MS.gene066185:CDS
TACGGGAACAACCGCCGACC+GGG 0.531411 2.2:-28905552 None:intergenic
TTTGGATCGTTGGATTGGGA+AGG 0.535121 2.2:+28905865 MS.gene066185:CDS
TTACGGGAACAACCGCCGAC+CGG 0.535490 2.2:-28905553 None:intergenic
CTTCGTAACGTCCCGGTCGG+CGG 0.554145 2.2:+28905541 MS.gene066185:CDS
ATGCAAGAAATTCATTCGAT+GGG 0.560936 2.2:+28905316 MS.gene066185:CDS
GCTTGCTGCTGAAACGGTGA+TGG 0.562137 2.2:-28905608 None:intergenic
TGGCGTCCTTCGTAACGTCC+CGG 0.563071 2.2:+28905534 MS.gene066185:CDS
ATTCATTTGATAACTGCTGA+TGG 0.567874 2.2:-28905843 None:intergenic
GACTTATCAGCTTCCAGTGG+AGG 0.568248 2.2:-28905643 None:intergenic
GTCCTTCGTAACGTCCCGGT+CGG 0.573615 2.2:+28905538 MS.gene066185:CDS
GATTCTCATTCTAACAGTGA+AGG 0.590117 2.2:+28905667 MS.gene066185:CDS
TGTGGTACAGCACGGAGACT+CGG 0.595362 2.2:+28905810 MS.gene066185:CDS
CGCCGACCGGGACGTTACGA+AGG 0.605026 2.2:-28905540 None:intergenic
TATAACAGAGTTAACCCTAA+CGG 0.613913 2.2:-28905695 None:intergenic
AGAAATGACGAGGCTGAGAG+AGG 0.614353 2.2:-28905480 None:intergenic
CAAGAAATTCATTCGATGGG+AGG 0.621582 2.2:+28905319 MS.gene066185:CDS
TAAATCAAAGAAATCAAAGA+CGG 0.630139 2.2:+28905573 MS.gene066185:CDS
TGGTTGAACTTGAAGTTACG+AGG 0.652946 2.2:-28905747 None:intergenic
CACCTATAGTCCAGTAGCGA+CGG 0.655105 2.2:-28905513 None:intergenic
TGGGAATGATCAAGTACATG+TGG 0.657314 2.2:+28905792 MS.gene066185:CDS
CAAGTACATGTGGTACAGCA+CGG 0.660421 2.2:+28905802 MS.gene066185:CDS
CGTCGCTACTGGACTATAGG+TGG 0.666718 2.2:+28905514 MS.gene066185:CDS
TCTGACTTATCAGCTTCCAG+TGG 0.676214 2.2:-28905646 None:intergenic
CAGTTCTTACAGAAATGACG+AGG 0.702494 2.2:-28905490 None:intergenic
TGTCCAGTAAGACTCATCAA+CGG 0.726070 2.2:-28905899 None:intergenic
GTGTTGAAGGAATTACAGCG+AGG 0.733816 2.2:-28905430 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATCAAAGAAATCAAAGA+CGG + chr2.2:28905573-28905592 MS.gene066185:CDS 20.0%
!!! TCTTTGATTTCTTTGATTTA+CGG - chr2.2:28905573-28905592 None:intergenic 20.0%
!! CTTTGATTTCTTTGATTTAC+GGG - chr2.2:28905572-28905591 None:intergenic 25.0%
ATCAAATGAATTTGGATCGT+TGG + chr2.2:28905855-28905874 MS.gene066185:CDS 30.0%
ATGCAAGAAATTCATTCGAT+GGG + chr2.2:28905316-28905335 MS.gene066185:CDS 30.0%
CAGCAGTTATCAAATGAATT+TGG + chr2.2:28905847-28905866 MS.gene066185:CDS 30.0%
TATAACAGAGTTAACCCTAA+CGG - chr2.2:28905698-28905717 None:intergenic 30.0%
! ATGATAATTCTGGTTATGGT+GGG - chr2.2:28905386-28905405 None:intergenic 30.0%
! ATTCATTTGATAACTGCTGA+TGG - chr2.2:28905846-28905865 None:intergenic 30.0%
! GATGATGATAATTCTGGTTA+TGG - chr2.2:28905390-28905409 None:intergenic 30.0%
! GATGATGATGATGATAATTC+TGG - chr2.2:28905396-28905415 None:intergenic 30.0%
!! ACCAATGATGTTGTTTCTTA+TGG + chr2.2:28905766-28905785 MS.gene066185:CDS 30.0%
!! CCAATGATGTTGTTTCTTAT+GGG + chr2.2:28905767-28905786 MS.gene066185:CDS 30.0%
!!! TGGAAATCTTTTTTGTGTTG+AGG + chr2.2:28905342-28905361 MS.gene066185:CDS 30.0%
ATGAATTTGGATCGTTGGAT+TGG + chr2.2:28905860-28905879 MS.gene066185:CDS 35.0%
CCCATAAGAAACAACATCAT+TGG - chr2.2:28905770-28905789 None:intergenic 35.0%
GATTCTCATTCTAACAGTGA+AGG + chr2.2:28905667-28905686 MS.gene066185:CDS 35.0%
TCAGGGAAGATGAAAAAGAA+AGG - chr2.2:28905959-28905978 None:intergenic 35.0%
TGAATTTGGATCGTTGGATT+GGG + chr2.2:28905861-28905880 MS.gene066185:CDS 35.0%
! GATGATAATTCTGGTTATGG+TGG - chr2.2:28905387-28905406 None:intergenic 35.0%
! GTAGCAGAATTTTGTGTTGA+AGG - chr2.2:28905446-28905465 None:intergenic 35.0%
!! ATGTTGTTTCTTATGGGCTT+GGG + chr2.2:28905773-28905792 MS.gene066185:CDS 35.0%
CAAGAAATTCATTCGATGGG+AGG + chr2.2:28905319-28905338 MS.gene066185:CDS 40.0%
CAGTTCTTACAGAAATGACG+AGG - chr2.2:28905493-28905512 None:intergenic 40.0%
TGGGAATGATCAAGTACATG+TGG + chr2.2:28905792-28905811 MS.gene066185:CDS 40.0%
TGGTTGAACTTGAAGTTACG+AGG - chr2.2:28905750-28905769 None:intergenic 40.0%
TGTCCAGTAAGACTCATCAA+CGG - chr2.2:28905902-28905921 None:intergenic 40.0%
!! GATGTTGTTTCTTATGGGCT+TGG + chr2.2:28905772-28905791 MS.gene066185:CDS 40.0%
ACAGTGAAGGTTCAACCGTT+AGG + chr2.2:28905680-28905699 MS.gene066185:CDS 45.0%
CAAGTACATGTGGTACAGCA+CGG + chr2.2:28905802-28905821 MS.gene066185:CDS 45.0%
CAGTGAAGGTTCAACCGTTA+GGG + chr2.2:28905681-28905700 MS.gene066185:CDS 45.0%
CTTACTGGACACACTCTCAT+TGG + chr2.2:28905911-28905930 MS.gene066185:CDS 45.0%
GAAATTCATTCGATGGGAGG+TGG + chr2.2:28905322-28905341 MS.gene066185:CDS 45.0%
GTGTTGAAGGAATTACAGCG+AGG - chr2.2:28905433-28905452 None:intergenic 45.0%
TCTGACTTATCAGCTTCCAG+TGG - chr2.2:28905649-28905668 None:intergenic 45.0%
! TTTGGATCGTTGGATTGGGA+AGG + chr2.2:28905865-28905884 MS.gene066185:CDS 45.0%
AGAAATGACGAGGCTGAGAG+AGG - chr2.2:28905483-28905502 None:intergenic 50.0%
CACCTATAGTCCAGTAGCGA+CGG - chr2.2:28905516-28905535 None:intergenic 50.0%
GACTTATCAGCTTCCAGTGG+AGG - chr2.2:28905646-28905665 None:intergenic 50.0%
TTATCAGCTTCCAGTGGAGG+TGG - chr2.2:28905643-28905662 None:intergenic 50.0%
! GCACCGTTGATGAGTCTTAC+TGG + chr2.2:28905896-28905915 MS.gene066185:CDS 50.0%
CGTCGCTACTGGACTATAGG+TGG + chr2.2:28905514-28905533 MS.gene066185:CDS 55.0%
GTAAGAACTGCCGTCGCTAC+TGG + chr2.2:28905503-28905522 MS.gene066185:CDS 55.0%
TGCCGTCGCTACTGGACTAT+AGG + chr2.2:28905511-28905530 MS.gene066185:CDS 55.0%
TGTGGTACAGCACGGAGACT+CGG + chr2.2:28905810-28905829 MS.gene066185:CDS 55.0%
! GCTTGCTGCTGAAACGGTGA+TGG - chr2.2:28905611-28905630 None:intergenic 55.0%
! GGATCGTTGGATTGGGAAGG+TGG + chr2.2:28905868-28905887 MS.gene066185:CDS 55.0%
! TGGCGTGCTTGCTGCTGAAA+CGG - chr2.2:28905617-28905636 None:intergenic 55.0%
GCTTCCAGTGGAGGTGGTGT+TGG - chr2.2:28905637-28905656 None:intergenic 60.0%
GTCCTTCGTAACGTCCCGGT+CGG + chr2.2:28905538-28905557 MS.gene066185:CDS 60.0%
TGGCGTCCTTCGTAACGTCC+CGG + chr2.2:28905534-28905553 MS.gene066185:CDS 60.0%
TTACGGGAACAACCGCCGAC+CGG - chr2.2:28905556-28905575 None:intergenic 60.0%
CACGCCAACACCACCTCCAC+TGG + chr2.2:28905630-28905649 MS.gene066185:CDS 65.0%
CTTCGTAACGTCCCGGTCGG+CGG + chr2.2:28905541-28905560 MS.gene066185:CDS 65.0%
TACGGGAACAACCGCCGACC+GGG - chr2.2:28905555-28905574 None:intergenic 65.0%
CGCCGACCGGGACGTTACGA+AGG - chr2.2:28905543-28905562 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 28905316 28905978 28905316 ID=MS.gene066185
chr2.2 mRNA 28905316 28905978 28905316 ID=MS.gene066185.t1;Parent=MS.gene066185
chr2.2 exon 28905316 28905978 28905316 ID=MS.gene066185.t1.exon1;Parent=MS.gene066185.t1
chr2.2 CDS 28905316 28905978 28905316 ID=cds.MS.gene066185.t1;Parent=MS.gene066185.t1
Gene Sequence

>MS.gene066185

ATGCAAGAAATTCATTCGATGGGAGGTGGAAATCTTTTTTGTGTTGAGGAAGATAGAAGAATTGAAACCCACCATAACCAGAATTATCATCATCATCATCTTGCTTTGAAATGTCCTCGCTGTAATTCCTTCAACACAAAATTCTGCTACTATAACAACTACAACCTCTCTCAGCCTCGTCATTTCTGTAAGAACTGCCGTCGCTACTGGACTATAGGTGGCGTCCTTCGTAACGTCCCGGTCGGCGGTTGTTCCCGTAAATCAAAGAAATCAAAGACGGCATCATCTTCTTCCATCACCGTTTCAGCAGCAAGCACGCCAACACCACCTCCACTGGAAGCTGATAAGTCAGATTCTCATTCTAACAGTGAAGGTTCAACCGTTAGGGTTAACTCTGTTATAGAAAATGAACAAGTTAATAGCTTCAGCAACCTCGTAACTTCAAGTTCAACCAATGATGTTGTTTCTTATGGGCTTGGGAATGATCAAGTACATGTGGTACAGCACGGAGACTCGGTGATGCTCAACCATCAGCAGTTATCAAATGAATTTGGATCGTTGGATTGGGAAGGTGGTGCTAGCACCGTTGATGAGTCTTACTGGACACACTCTCATTGGTCAGATCATGTCAATCATTCATCCTTTCTTTTTCATCTTCCCTGA
Protein sequence

>MS.gene066185.t1

MQEIHSMGGGNLFCVEEDRRIETHHNQNYHHHHLALKCPRCNSFNTKFCYYNNYNLSQPRHFCKNCRRYWTIGGVLRNVPVGGCSRKSKKSKTASSSSITVSAASTPTPPPLEADKSDSHSNSEGSTVRVNSVIENEQVNSFSNLVTSSSTNDVVSYGLGNDQVHVVQHGDSVMLNHQQLSNEFGSLDWEGGASTVDESYWTHSHWSDHVNHSSFLFHLP