AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066241


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066241.t1 MTR_1g099310 77.931 290 7 2 17 249 7 296 8.19e-153 427
MS.gene066241.t1 MTR_1g099290 65.398 289 42 4 17 247 8 296 2.36e-120 355
MS.gene066241.t1 MTR_8g055940 52.577 291 78 5 17 249 9 297 7.72e-95 280
MS.gene066241.t1 MTR_0027s0260 52.234 291 80 5 17 249 9 298 7.62e-94 278
MS.gene066241.t1 MTR_1g099320 53.957 278 67 6 32 249 21 297 3.72e-89 266
MS.gene066241.t1 MTR_5g043550 51.027 292 81 5 17 249 6 294 3.22e-88 263
MS.gene066241.t1 MTR_8g467650 47.635 296 92 6 15 249 5 298 5.54e-78 237
MS.gene066241.t1 MTR_7g116350 46.690 287 80 6 22 249 12 284 3.35e-74 241
MS.gene066241.t1 MTR_1g099350 46.763 278 85 7 31 249 18 291 4.77e-72 222
MS.gene066241.t1 MTR_7g116850 45.704 291 94 7 22 249 37 326 1.96e-71 221
MS.gene066241.t1 MTR_2g102020 43.771 297 95 9 24 249 18 313 4.92e-64 203
MS.gene066241.t1 MTR_6g023340 55.030 169 70 4 29 193 20 186 1.67e-59 190
MS.gene066241.t1 MTR_6g023340 56.897 58 24 1 193 249 245 302 1.74e-11 63.2
MS.gene066241.t1 MTR_2g040830 42.199 282 97 8 31 249 22 300 3.59e-58 187
MS.gene066241.t1 MTR_2g040690 42.199 282 97 8 31 249 24 302 4.27e-58 187
MS.gene066241.t1 MTR_2g040740 55.152 165 69 4 30 192 21 182 4.39e-51 169
MS.gene066241.t1 MTR_2g040850 40.708 226 97 4 30 246 21 218 2.99e-46 154
MS.gene066241.t1 MTR_2g040650 37.589 282 106 9 31 249 22 296 6.37e-43 148
MS.gene066241.t1 MTR_2g040870 48.125 160 79 3 25 183 13 169 1.09e-41 140
MS.gene066241.t1 MTR_2g040890 42.169 166 85 5 18 175 4 166 1.54e-33 120
MS.gene066241.t1 MTR_4g063180 41.117 197 72 7 44 240 30 182 2.24e-28 106
MS.gene066241.t1 MTR_4g077310 78.182 55 11 1 192 246 37 90 5.18e-21 84.7
MS.gene066241.t1 MTR_2g040880 54.386 57 26 0 193 249 61 117 7.34e-13 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066241.t1 AT5G24090 47.267 311 90 7 1 249 4 302 6.20e-86 258

Find 61 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGAACCTTCATTTCCATTT+TGG 0.201244 1.1:-71496985 None:intergenic
GACCTTGCAAGAGCACTTAA+TGG 0.258905 1.1:+71497364 MS.gene066241:CDS
TGCAGGAATTGCTGTCTATT+GGG 0.293692 1.1:+71496961 MS.gene066241:CDS
CTGCAGGAATTGCTGTCTAT+TGG 0.299764 1.1:+71496960 MS.gene066241:CDS
TTACCTATGGAACAATTTCT+TGG 0.301794 1.1:+71497255 MS.gene066241:CDS
ACCATTAGGTGATGCTGTTT+TGG 0.309129 1.1:+71497297 MS.gene066241:CDS
ATCAAGGGTTCTGCTAAATA+TGG 0.352843 1.1:+71497626 MS.gene066241:CDS
CTCTCTCTCGGAGGTGGTGC+CGG 0.355247 1.1:+71497187 MS.gene066241:CDS
AATATGGTGGTGTCATGATT+TGG 0.364242 1.1:+71497642 MS.gene066241:CDS
GGCCAGCAAGGTTTAGAGTT+GGG 0.367643 1.1:-71497084 None:intergenic
TCAGGTTCTTCCTGCTATCA+AGG 0.374119 1.1:+71497610 MS.gene066241:CDS
AAACTTTGTGCAACCGTTTG+AGG 0.377387 1.1:-71497119 None:intergenic
ACCCCAACTCTAAACCTTGC+TGG 0.394335 1.1:+71497082 MS.gene066241:CDS
CAAGGTTTAGAGTTGGGGTT+TGG 0.398633 1.1:-71497078 None:intergenic
GCTGTCTATTGGGGCCAAAA+TGG 0.403140 1.1:+71496971 MS.gene066241:CDS
CAGCATGAGAGGATTTGAAC+AGG 0.421892 1.1:-71496940 None:intergenic
TACCTATGGAACAATTTCTT+GGG 0.439074 1.1:+71497256 MS.gene066241:CDS
ACGTCATCTTCAAGACCATT+AGG 0.446236 1.1:+71497283 MS.gene066241:CDS
TGGGTATAGTAATGCTATCA+AGG 0.449430 1.1:+71497688 MS.gene066241:CDS
ATATGGTGGTGTCATGATTT+GGG 0.457395 1.1:+71497643 MS.gene066241:CDS
TCCAAAACAGCATCACCTAA+TGG 0.457612 1.1:-71497298 None:intergenic
TTTGTGCAACCGTTTGAGGC+TGG 0.457856 1.1:-71497115 None:intergenic
ATCAAAGTGTTGCTCTCTCT+CGG 0.468652 1.1:+71497175 MS.gene066241:CDS
TTCAAATCCTCTCATGCTGC+AGG 0.481583 1.1:+71496944 MS.gene066241:CDS
ACAGGGAAATGAGGAAAAGA+GGG 0.491765 1.1:-71496922 None:intergenic
AACAGGGAAATGAGGAAAAG+AGG 0.492417 1.1:-71496923 None:intergenic
GCCAGCAAGGTTTAGAGTTG+GGG 0.492427 1.1:-71497083 None:intergenic
CAGGTTCTTCCTGCTATCAA+GGG 0.501869 1.1:+71497611 MS.gene066241:CDS
CACAGGTTGCAAATTACCTA+TGG 0.509878 1.1:+71497242 MS.gene066241:CDS
TTAGCAGAACCCTTGATAGC+AGG 0.515382 1.1:-71497620 None:intergenic
CTATGGAACAATTTCTTGGG+TGG 0.517482 1.1:+71497259 MS.gene066241:CDS
TGGGGCCAAAATGGAAATGA+AGG 0.518821 1.1:+71496980 MS.gene066241:CDS
ATTTGGGATAGATTCAATGA+TGG 0.518849 1.1:+71497659 MS.gene066241:CDS
GGTTTGGCCATTGCCAAAAG+TGG 0.520401 1.1:-71497062 None:intergenic
TGAGAGTTCAAAACATCAGC+AGG 0.521909 1.1:-71497590 None:intergenic
AGGATTTGAACAGGGAAATG+AGG 0.523284 1.1:-71496931 None:intergenic
GTGTTGCTCTCTCTCGGAGG+TGG 0.537411 1.1:+71497181 MS.gene066241:CDS
GCAGGAATTGCTGTCTATTG+GGG 0.539336 1.1:+71496962 MS.gene066241:CDS
TGAGGAAAGAGAGTAGCTTC+CGG 0.540465 1.1:-71497206 None:intergenic
CATTGTGATCCAGCCTCAAA+CGG 0.541735 1.1:+71497106 MS.gene066241:CDS
TGGCCAGCAAGGTTTAGAGT+TGG 0.542841 1.1:-71497085 None:intergenic
AGCAATTCCTGCAGCATGAG+AGG 0.550272 1.1:-71496951 None:intergenic
CTCAGCTGATGATGCCACAC+AGG 0.550683 1.1:+71497225 MS.gene066241:CDS
AACCATTAAGTGCTCTTGCA+AGG 0.563162 1.1:-71497366 None:intergenic
GGAGCAGCAGCATCATTAGC+AGG 0.564812 1.1:-71497551 None:intergenic
AGATTCAATGATGGACAAAG+TGG 0.579005 1.1:+71497668 MS.gene066241:CDS
CACCCAAGAAATTGTTCCAT+AGG 0.580557 1.1:-71497258 None:intergenic
CTGGATCACAATGGCCAGCA+AGG 0.581308 1.1:-71497096 None:intergenic
GATGCTGCTGCTCCAAGTGG+TGG 0.591043 1.1:+71497560 MS.gene066241:CDS
GGGTATAGTAATGCTATCAA+GGG 0.607448 1.1:+71497689 MS.gene066241:CDS
GCAGGAATAAAACCACCACT+TGG 0.623738 1.1:-71497572 None:intergenic
AAGGGTTCTGCTAAATATGG+TGG 0.631653 1.1:+71497629 MS.gene066241:CDS
ATTCAAACTTGTCAAAGCAA+CGG 0.634122 1.1:+71497151 MS.gene066241:CDS
CTGTGTGGCATCATCAGCTG+AGG 0.646251 1.1:-71497224 None:intergenic
TGTGTTGCAAGCATCTGCTA+AGG 0.648082 1.1:-71497005 None:intergenic
AATGATGCTGCTGCTCCAAG+TGG 0.649731 1.1:+71497557 MS.gene066241:CDS
GATTCAATGATGGACAAAGT+GGG 0.659679 1.1:+71497669 MS.gene066241:CDS
AGCATGAGAGGATTTGAACA+GGG 0.680167 1.1:-71496939 None:intergenic
TAGGTAATTTGCAACCTGTG+TGG 0.691863 1.1:-71497239 None:intergenic
AAAGTGTTGCTCTCTCTCGG+AGG 0.693552 1.1:+71497178 MS.gene066241:CDS
CGTTTGAGGCTGGATCACAA+TGG 0.735602 1.1:-71497105 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTGATTTTGATATTGAAGC+TGG + chr1.1:71497325-71497344 MS.gene066241:CDS 25.0%
!!! TTAATGGTTTTAGTTCACAA+AGG + chr1.1:71497380-71497399 MS.gene066241:CDS 25.0%
ATTCAAACTTGTCAAAGCAA+CGG + chr1.1:71497151-71497170 MS.gene066241:CDS 30.0%
CTAACAATCTTGTTAATGCT+TGG + chr1.1:71497483-71497502 MS.gene066241:intron 30.0%
TACCTATGGAACAATTTCTT+GGG + chr1.1:71497256-71497275 MS.gene066241:CDS 30.0%
TTACCTATGGAACAATTTCT+TGG + chr1.1:71497255-71497274 MS.gene066241:CDS 30.0%
TTGTTAATGCTTGGAATCAA+TGG + chr1.1:71497492-71497511 MS.gene066241:intron 30.0%
! ATAGCTTTTCTATCCACTTT+TGG + chr1.1:71497049-71497068 MS.gene066241:CDS 30.0%
! ATTTGGGATAGATTCAATGA+TGG + chr1.1:71497659-71497678 MS.gene066241:CDS 30.0%
! CAAGCTAAACAAGTGTTTTT+AGG + chr1.1:71497524-71497543 MS.gene066241:intron 30.0%
ATCAAGGGTTCTGCTAAATA+TGG + chr1.1:71497626-71497645 MS.gene066241:CDS 35.0%
GGGTATAGTAATGCTATCAA+GGG + chr1.1:71497689-71497708 MS.gene066241:CDS 35.0%
TAGTGTTTCCACTTGAGTAT+TGG - chr1.1:71497466-71497485 None:intergenic 35.0%
TGGGTATAGTAATGCTATCA+AGG + chr1.1:71497688-71497707 MS.gene066241:CDS 35.0%
! AGGAACCTTCATTTCCATTT+TGG - chr1.1:71496988-71497007 None:intergenic 35.0%
!! AATATGGTGGTGTCATGATT+TGG + chr1.1:71497642-71497661 MS.gene066241:CDS 35.0%
!! AGATTCAATGATGGACAAAG+TGG + chr1.1:71497668-71497687 MS.gene066241:CDS 35.0%
!! ATATGGTGGTGTCATGATTT+GGG + chr1.1:71497643-71497662 MS.gene066241:CDS 35.0%
!! GATTCAATGATGGACAAAGT+GGG + chr1.1:71497669-71497688 MS.gene066241:CDS 35.0%
!! GATTTTGATATTGAAGCTGG+TGG + chr1.1:71497328-71497347 MS.gene066241:CDS 35.0%
!! TTTCTATCCACTTTTGGCAA+TGG + chr1.1:71497055-71497074 MS.gene066241:CDS 35.0%
AAACTTTGTGCAACCGTTTG+AGG - chr1.1:71497122-71497141 None:intergenic 40.0%
AACAGGGAAATGAGGAAAAG+AGG - chr1.1:71496926-71496945 None:intergenic 40.0%
AACCATTAAGTGCTCTTGCA+AGG - chr1.1:71497369-71497388 None:intergenic 40.0%
AAGGGTTCTGCTAAATATGG+TGG + chr1.1:71497629-71497648 MS.gene066241:CDS 40.0%
ACAGGGAAATGAGGAAAAGA+GGG - chr1.1:71496925-71496944 None:intergenic 40.0%
ACGTCATCTTCAAGACCATT+AGG + chr1.1:71497283-71497302 MS.gene066241:CDS 40.0%
ACTGAATCCAAATGAGCATC+AGG - chr1.1:71497442-71497461 None:intergenic 40.0%
AGCATGAGAGGATTTGAACA+GGG - chr1.1:71496942-71496961 None:intergenic 40.0%
AGGATTTGAACAGGGAAATG+AGG - chr1.1:71496934-71496953 None:intergenic 40.0%
ATTCAGTGCCAATACTCAAG+TGG + chr1.1:71497455-71497474 MS.gene066241:intron 40.0%
CACAGGTTGCAAATTACCTA+TGG + chr1.1:71497242-71497261 MS.gene066241:CDS 40.0%
CACCCAAGAAATTGTTCCAT+AGG - chr1.1:71497261-71497280 None:intergenic 40.0%
CTATGGAACAATTTCTTGGG+TGG + chr1.1:71497259-71497278 MS.gene066241:CDS 40.0%
CTGAATCCAAATGAGCATCA+GGG - chr1.1:71497441-71497460 None:intergenic 40.0%
TCCAAAACAGCATCACCTAA+TGG - chr1.1:71497301-71497320 None:intergenic 40.0%
TGAGAGTTCAAAACATCAGC+AGG - chr1.1:71497593-71497612 None:intergenic 40.0%
TGCAGGAATTGCTGTCTATT+GGG + chr1.1:71496961-71496980 MS.gene066241:CDS 40.0%
! TAGGTAATTTGCAACCTGTG+TGG - chr1.1:71497242-71497261 None:intergenic 40.0%
!! ACCATTAGGTGATGCTGTTT+TGG + chr1.1:71497297-71497316 MS.gene066241:CDS 40.0%
!! ATCAAAGTGTTGCTCTCTCT+CGG + chr1.1:71497175-71497194 MS.gene066241:CDS 40.0%
!! TGCTGATGTTTTGAACTCTC+AGG + chr1.1:71497592-71497611 MS.gene066241:CDS 40.0%
!!! TTAGGTGATGCTGTTTTGGA+TGG + chr1.1:71497301-71497320 MS.gene066241:CDS 40.0%
CAGCATGAGAGGATTTGAAC+AGG - chr1.1:71496943-71496962 None:intergenic 45.0%
CAGGTTCTTCCTGCTATCAA+GGG + chr1.1:71497611-71497630 MS.gene066241:CDS 45.0%
CATTGTGATCCAGCCTCAAA+CGG + chr1.1:71497106-71497125 MS.gene066241:CDS 45.0%
CTGCAGGAATTGCTGTCTAT+TGG + chr1.1:71496960-71496979 MS.gene066241:CDS 45.0%
GCAGGAATAAAACCACCACT+TGG - chr1.1:71497575-71497594 None:intergenic 45.0%
GCAGGAATTGCTGTCTATTG+GGG + chr1.1:71496962-71496981 MS.gene066241:CDS 45.0%
TCAGGTTCTTCCTGCTATCA+AGG + chr1.1:71497610-71497629 MS.gene066241:CDS 45.0%
TCCAAATGAGCATCAGGGTA+AGG - chr1.1:71497436-71497455 None:intergenic 45.0%
TCCTTACCCTGATGCTCATT+TGG + chr1.1:71497432-71497451 MS.gene066241:intron 45.0%
TGAGGAAAGAGAGTAGCTTC+CGG - chr1.1:71497209-71497228 None:intergenic 45.0%
TGGGGCCAAAATGGAAATGA+AGG + chr1.1:71496980-71496999 MS.gene066241:CDS 45.0%
TGTGTTGCAAGCATCTGCTA+AGG - chr1.1:71497008-71497027 None:intergenic 45.0%
TTCAAATCCTCTCATGCTGC+AGG + chr1.1:71496944-71496963 MS.gene066241:CDS 45.0%
! CAAGGTTTAGAGTTGGGGTT+TGG - chr1.1:71497081-71497100 None:intergenic 45.0%
! TTAGCAGAACCCTTGATAGC+AGG - chr1.1:71497623-71497642 None:intergenic 45.0%
!! GACCTTGCAAGAGCACTTAA+TGG + chr1.1:71497364-71497383 MS.gene066241:CDS 45.0%
AATGATGCTGCTGCTCCAAG+TGG + chr1.1:71497557-71497576 MS.gene066241:CDS 50.0%
ACCCCAACTCTAAACCTTGC+TGG + chr1.1:71497082-71497101 MS.gene066241:CDS 50.0%
AGCAATTCCTGCAGCATGAG+AGG - chr1.1:71496954-71496973 None:intergenic 50.0%
GCATCAGGGTAAGGACATTG+AGG - chr1.1:71497427-71497446 None:intergenic 50.0%
GCTGTCTATTGGGGCCAAAA+TGG + chr1.1:71496971-71496990 MS.gene066241:CDS 50.0%
GGTTTGGCCATTGCCAAAAG+TGG - chr1.1:71497065-71497084 None:intergenic 50.0%
! CGTTTGAGGCTGGATCACAA+TGG - chr1.1:71497108-71497127 None:intergenic 50.0%
! GCCAGCAAGGTTTAGAGTTG+GGG - chr1.1:71497086-71497105 None:intergenic 50.0%
! GGCCAGCAAGGTTTAGAGTT+GGG - chr1.1:71497087-71497106 None:intergenic 50.0%
! TGGCCAGCAAGGTTTAGAGT+TGG - chr1.1:71497088-71497107 None:intergenic 50.0%
! TTTGTGCAACCGTTTGAGGC+TGG - chr1.1:71497118-71497137 None:intergenic 50.0%
!! AAAGTGTTGCTCTCTCTCGG+AGG + chr1.1:71497178-71497197 MS.gene066241:CDS 50.0%
CTGGATCACAATGGCCAGCA+AGG - chr1.1:71497099-71497118 None:intergenic 55.0%
CTGTGTGGCATCATCAGCTG+AGG - chr1.1:71497227-71497246 None:intergenic 55.0%
GGAGCAGCAGCATCATTAGC+AGG - chr1.1:71497554-71497573 None:intergenic 55.0%
! CTCAGCTGATGATGCCACAC+AGG + chr1.1:71497225-71497244 MS.gene066241:CDS 55.0%
GATGCTGCTGCTCCAAGTGG+TGG + chr1.1:71497560-71497579 MS.gene066241:CDS 60.0%
! GTGTTGCTCTCTCTCGGAGG+TGG + chr1.1:71497181-71497200 MS.gene066241:CDS 60.0%
CTCTCTCTCGGAGGTGGTGC+CGG + chr1.1:71497187-71497206 MS.gene066241:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 71496857 71497721 71496857 ID=MS.gene066241
chr1.1 mRNA 71496857 71497721 71496857 ID=MS.gene066241.t1;Parent=MS.gene066241
chr1.1 exon 71496857 71497430 71496857 ID=MS.gene066241.t1.exon1;Parent=MS.gene066241.t1
chr1.1 CDS 71496857 71497430 71496857 ID=cds.MS.gene066241.t1;Parent=MS.gene066241.t1
chr1.1 exon 71497546 71497721 71497546 ID=MS.gene066241.t1.exon2;Parent=MS.gene066241.t1
chr1.1 CDS 71497546 71497721 71497546 ID=cds.MS.gene066241.t1;Parent=MS.gene066241.t1
Gene Sequence

>MS.gene066241

ATGAGCATAAAAAAATATTTAATTGCATTAACTTTTGAAATATATCTTATAAAAATACAACAATTCCCTCTTTTCCTCATTTCCCTGTTCAAATCCTCTCATGCTGCAGGAATTGCTGTCTATTGGGGCCAAAATGGAAATGAAGGTTCCTTAGCAGATGCTTGCAACACAAACAACTATCAATTTGTAAACATAGCTTTTCTATCCACTTTTGGCAATGGCCAAACCCCAACTCTAAACCTTGCTGGCCATTGTGATCCAGCCTCAAACGGTTGCACAAAGTTTAGTAGTGAAATTCAAACTTGTCAAAGCAACGGCATCAAAGTGTTGCTCTCTCTCGGAGGTGGTGCCGGAAGCTACTCTCTTTCCTCAGCTGATGATGCCACACAGGTTGCAAATTACCTATGGAACAATTTCTTGGGTGGCACGTCATCTTCAAGACCATTAGGTGATGCTGTTTTGGATGGAATTGATTTTGATATTGAAGCTGGTGGTGAACACTATGATGACCTTGCAAGAGCACTTAATGGTTTTAGTTCACAAAGGAGAGTTTACTTGTCTGCAGCACCTCAATGTCCTTACCCTGATGCTCATTTGGATTCAGTGCCAATACTCAAGTGGAAACACTAACAATCTTGTTAATGCTTGGAATCAATGGACTTCAAGTCAAGCTAAACAAGTGTTTTTAGGTGTACCTGCTAATGATGCTGCTGCTCCAAGTGGTGGTTTTATTCCTGCTGATGTTTTGAACTCTCAGGTTCTTCCTGCTATCAAGGGTTCTGCTAAATATGGTGGTGTCATGATTTGGGATAGATTCAATGATGGACAAAGTGGGTATAGTAATGCTATCAAGGGCAGTGTTTAA

Protein sequence

>MS.gene066241.t1

MSIKKYLIALTFEIYLIKIQQFPLFLISLFKSSHAAGIAVYWGQNGNEGSLADACNTNNYQFVNIAFLSTFGNGQTPTLNLAGHCDPASNGCTKFSSEIQTCQSNGIKVLLSLGGGAGSYSLSSADDATQVANYLWNNFLGGTSSSRPLGDAVLDGIDFDIEAGGEHYDDLARALNGFSSQRRVYLSAAPQCVPANDAAAPSGGFIPADVLNSQVLPAIKGSAKYGGVMIWDRFNDGQSGYSNAIKGSV