AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066528


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066528.t1 MTR_4g119880 98.899 454 5 0 1 454 1 454 0.0 943
MS.gene066528.t1 MTR_7g108675 78.373 467 87 3 1 454 1 466 0.0 754
MS.gene066528.t1 MTR_8g058553 78.072 415 77 3 1 402 1 414 0.0 669
MS.gene066528.t1 MTR_3g037340 58.469 431 154 4 10 440 36 441 0.0 546
MS.gene066528.t1 MTR_4g108940 57.240 442 165 4 8 449 28 445 0.0 545
MS.gene066528.t1 MTR_5g015260 42.526 388 181 7 79 431 66 446 8.83e-111 334
MS.gene066528.t1 MTR_4g097240 39.486 428 206 8 27 421 19 426 1.93e-108 328
MS.gene066528.t1 MTR_4g097230 43.466 352 160 6 107 424 69 415 1.37e-101 310
MS.gene066528.t1 MTR_4g108950 49.606 127 20 1 285 411 1 83 2.66e-33 121
MS.gene066528.t1 MTR_4g132910 82.051 39 7 0 357 395 582 620 2.16e-13 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066528.t1 AT3G62720 77.874 461 93 4 1 454 1 459 0.0 763
MS.gene066528.t1 AT3G62720 77.874 461 93 4 1 454 1 459 0.0 763
MS.gene066528.t1 AT4G02500 79.914 463 81 3 1 454 1 460 0.0 761
MS.gene066528.t1 AT1G74380 58.879 428 153 5 13 440 42 446 0.0 553
MS.gene066528.t1 AT5G07720 58.469 431 155 5 10 440 39 445 0.0 551
MS.gene066528.t1 AT1G18690 52.292 480 185 8 4 440 26 504 4.90e-180 513
MS.gene066528.t1 AT1G18690 52.292 480 185 8 4 440 26 504 4.90e-180 513
MS.gene066528.t1 AT1G18690 52.292 480 185 8 4 440 26 504 4.90e-180 513
MS.gene066528.t1 AT1G18690 52.292 480 185 8 4 440 26 504 4.90e-180 513
MS.gene066528.t1 AT2G22900 42.120 349 159 5 107 417 89 432 1.92e-96 297
MS.gene066528.t1 AT4G37690 40.974 349 163 6 107 417 72 415 1.35e-94 292
MS.gene066528.t1 AT4G38310 44.444 108 56 2 107 213 13 117 6.40e-28 107

Find 0 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTAGGATTAATAAAATT+AGG - 32584:6115-6134 MS.gene066528:intergenic 10.0%
!! TAATAAAATTAGGATAATCA+GGG - 32584:6105-6124 MS.gene066528:intergenic 15.0%
!! TTAATAAAATTAGGATAATC+AGG - 32584:6106-6125 MS.gene066528:intergenic 15.0%
!! AGAATAAGATTGATTATTGT+AGG + 32584:6218-6237 MS.gene066528:CDS 20.0%
!! ATTATTATTTGCATGGTTAT+TGG + 32584:6716-6735 MS.gene066528:CDS 20.0%
!! TTATTATTTGCATGGTTATT+GGG + 32584:6717-6736 MS.gene066528:CDS 20.0%
! GAGAATCATTATTATTTGCA+TGG + 32584:6709-6728 MS.gene066528:CDS 25.0%
! TATTATTTGCATGGTTATTG+GGG + 32584:6718-6737 MS.gene066528:CDS 25.0%
! TGATTTATGATGAGAAGAAT+TGG + 32584:6461-6480 MS.gene066528:CDS 25.0%
!! GATTGAGATTTTCTATAACA+TGG + 32584:6249-6268 MS.gene066528:CDS 25.0%
AACAAATAATGATCACCAAC+AGG - 32584:6187-6206 MS.gene066528:intergenic 30.0%
AACAATAGCTTCCTAATCAA+AGG - 32584:6316-6335 MS.gene066528:intergenic 30.0%
AATCAGATATTGCAGATGTA+TGG + 32584:6913-6932 MS.gene066528:CDS 30.0%
ACTTGATATTCTTGATGCTT+GGG + 32584:6531-6550 MS.gene066528:CDS 30.0%
AGTTCATCTTTAACATCAAG+AGG - 32584:7000-7019 MS.gene066528:intergenic 30.0%
ATCTGCTATGGTTTATTTGT+TGG + 32584:6648-6667 MS.gene066528:CDS 30.0%
ATGATTGAGAATTATCATCC+TGG + 32584:6766-6785 MS.gene066528:CDS 30.0%
ATTATTTGCATGGTTATTGG+GGG + 32584:6719-6738 MS.gene066528:CDS 30.0%
ATTGATTATTGTAGGCTTCA+TGG + 32584:6226-6245 MS.gene066528:CDS 30.0%
CTTGATGTTAAAGATGAACT+TGG + 32584:7000-7019 MS.gene066528:CDS 30.0%
TATGATGAGAAGAATTGGAT+TGG + 32584:6466-6485 MS.gene066528:CDS 30.0%
TGATGCTATGTTTACTGATA+TGG + 32584:6375-6394 MS.gene066528:CDS 30.0%
TTGATTATTGTAGGCTTCAT+GGG + 32584:6227-6246 MS.gene066528:CDS 30.0%
! ATAATTTTGTGATGCATGGT+TGG + 32584:6431-6450 MS.gene066528:CDS 30.0%
! ATGGATCGTGCTTATAATTT+TGG + 32584:6886-6905 MS.gene066528:CDS 30.0%
! GATCATAATTTTGTGATGCA+TGG + 32584:6427-6446 MS.gene066528:CDS 30.0%
!! AATTGGATTGGTTTGAATAC+TGG + 32584:6478-6497 MS.gene066528:CDS 30.0%
!!! ATACTGGAAGTTTTTTGTTG+AGG + 32584:6494-6513 MS.gene066528:CDS 30.0%
AAAAGAACACGTGGCTTATT+AGG - 32584:6130-6149 MS.gene066527:intergenic 35.0%
ATTGAAATTCTTCGAGAACG+CGG - 32584:5913-5932 MS.gene066528:intergenic 35.0%
CACTTGATATTCTTGATGCT+TGG + 32584:6530-6549 MS.gene066528:CDS 35.0%
TAATCACCAAATTTCCCACA+AGG - 32584:6841-6860 MS.gene066528:intergenic 35.0%
TGTAAACCTTGTGGGAAATT+TGG + 32584:6832-6851 MS.gene066528:CDS 35.0%
! ATCCTGAAGTTGAGTTTTTG+TGG + 32584:6341-6360 MS.gene066528:CDS 35.0%
! ATGGTTTATTTGTTGGCAAC+TGG + 32584:6655-6674 MS.gene066528:CDS 35.0%
! GAGAATTATCATCCTGGTTT+TGG + 32584:6772-6791 MS.gene066528:CDS 35.0%
! TGGTTTATTTGTTGGCAACT+GGG + 32584:6656-6675 MS.gene066528:CDS 35.0%
! TTTTGTTGAGGAATTGTCAG+TGG + 32584:6506-6525 MS.gene066528:CDS 35.0%
!! AACTTGATAGCTTTGAAAGC+AGG - 32584:7036-7055 MS.gene066528:intergenic 35.0%
!! TGAGAGGTGTTTGAAACAAA+TGG + 32584:6867-6886 MS.gene066528:CDS 35.0%
!!! ATTTTGGGACCAAGTGTATA+AGG - 32584:6040-6059 MS.gene066528:intergenic 35.0%
!!! GTTATCGTTGTTGTTTTCTG+TGG - 32584:5936-5955 MS.gene066528:intergenic 35.0%
AAACCGTGTGAAAATCCTGT+TGG + 32584:6169-6188 MS.gene066528:CDS 40.0%
AAGCTGGGAAGATTCTTACT+AGG + 32584:6587-6606 MS.gene066528:CDS 40.0%
AGCAGATGATCAATCTGCTA+TGG + 32584:6636-6655 MS.gene066528:CDS 40.0%
AGCTGGGAAGATTCTTACTA+GGG + 32584:6588-6607 MS.gene066528:CDS 40.0%
ATCCTCAAAGATGAAGAAGC+TGG + 32584:5986-6005 MS.gene066528:CDS 40.0%
CACCACAAAAACTCAACTTC+AGG - 32584:6346-6365 MS.gene066528:intergenic 40.0%
CCAGTAACTAAAAGAACACG+TGG - 32584:6139-6158 MS.gene066528:intergenic 40.0%
GATTGTCGAGATATCGAAAG+TGG - 32584:5969-5988 MS.gene066528:intergenic 40.0%
GGCTTTACTTGATGCTGAAA+TGG + 32584:6270-6289 MS.gene066528:CDS 40.0%
GTAGAAGAGTGAAGAGAGTT+AGG + 32584:6959-6978 MS.gene066528:CDS 40.0%
GTTTACTGATATGGCTTTCG+AGG + 32584:6384-6403 MS.gene066528:CDS 40.0%
GTTTCAAACACCTCTCAACA+GGG - 32584:6864-6883 MS.gene066528:intergenic 40.0%
TAGGGAGTTAAAGAATCGAC+CGG + 32584:6606-6625 MS.gene066528:CDS 40.0%
TCATCTCTAACCTTCCCTTT+AGG - 32584:6568-6587 MS.gene066528:intergenic 40.0%
TGAACGTTGTTCATCCCAAT+CGG - 32584:6065-6084 MS.gene066528:intergenic 40.0%
TGATCATCTGCTTCGAAAAC+CGG - 32584:6628-6647 MS.gene066528:intergenic 40.0%
TGTTTCAAACACCTCTCAAC+AGG - 32584:6865-6884 MS.gene066528:intergenic 40.0%
TTACTTGATGCTGAAATGGC+TGG + 32584:6274-6293 MS.gene066528:CDS 40.0%
! ACAGGATTTTCACACGGTTT+AGG - 32584:6169-6188 MS.gene066528:intergenic 40.0%
! CTGAAGTTGAGTTTTTGTGG+TGG + 32584:6344-6363 MS.gene066528:CDS 40.0%
! TCACCAACAGGATTTTCACA+CGG - 32584:6175-6194 MS.gene066528:intergenic 40.0%
! TCATCCCAATCGGAGATTTT+GGG - 32584:6055-6074 MS.gene066528:intergenic 40.0%
! TTATTTGTTGGCAACTGGGA+GGG + 32584:6660-6679 MS.gene066528:CDS 40.0%
! TTCATCCCAATCGGAGATTT+TGG - 32584:6056-6075 MS.gene066528:intergenic 40.0%
! TTTATTTGTTGGCAACTGGG+AGG + 32584:6659-6678 MS.gene066528:CDS 40.0%
!! AGTTGAGTTTTTGTGGTGGA+TGG + 32584:6348-6367 MS.gene066528:CDS 40.0%
!! CATTGGTGACTCATTTTGTG+GGG + 32584:6809-6828 MS.gene066528:CDS 40.0%
!! CCACGTGTTCTTTTAGTTAC+TGG + 32584:6136-6155 MS.gene066528:CDS 40.0%
!! CCATTGGTGACTCATTTTGT+GGG + 32584:6808-6827 MS.gene066528:CDS 40.0%
!!! ATCCTGGTTTTGGTGATCAT+AGG + 32584:6782-6801 MS.gene066528:CDS 40.0%
!!! ATGCTGAAATGGCTGGTTTT+TGG + 32584:6281-6300 MS.gene066528:CDS 40.0%
!!! TGCTGAAATGGCTGGTTTTT+GGG + 32584:6282-6301 MS.gene066528:CDS 40.0%
CACCAGCTTCTTCATCTTTG+AGG - 32584:5991-6010 MS.gene066528:intergenic 45.0%
CACCTATGATCACCAAAACC+AGG - 32584:6787-6806 MS.gene066528:intergenic 45.0%
CCAAACACGCCTTATACACT+TGG + 32584:6028-6047 MS.gene066528:CDS 45.0%
CCAAGTGTATAAGGCGTGTT+TGG - 32584:6031-6050 MS.gene066528:intergenic 45.0%
CCCACAAAATGAGTCACCAA+TGG - 32584:6811-6830 MS.gene066528:intergenic 45.0%
GGAAGGTTAGAGATGAAGCT+GGG + 32584:6572-6591 MS.gene066528:CDS 45.0%
GGGCTAAGCTTCCTTTGATT+AGG + 32584:6302-6321 MS.gene066528:CDS 45.0%
TGATCCTTATAACGCTCCCA+CGG - 32584:6412-6431 MS.gene066528:intergenic 45.0%
TGGTCCCAAAATCTCCGATT+GGG + 32584:6048-6067 MS.gene066528:CDS 45.0%
TTGGTCCCAAAATCTCCGAT+TGG + 32584:6047-6066 MS.gene066528:CDS 45.0%
TTGTGGGGTGTAAACCTTGT+GGG + 32584:6824-6843 MS.gene066528:CDS 45.0%
TTTGTGGGGTGTAAACCTTG+TGG + 32584:6823-6842 MS.gene066528:CDS 45.0%
! GCATGGTTATTGGGGGATTT+TGG + 32584:6726-6745 MS.gene066528:CDS 45.0%
! TTGGTGATTACCCTGTTGAG+AGG + 32584:6851-6870 MS.gene066528:CDS 45.0%
!! ATCTCGTTGAGGTCTTGTTC+TGG - 32584:5848-5867 MS.gene066528:intergenic 45.0%
!! GCCATTGGTGACTCATTTTG+TGG + 32584:6807-6826 MS.gene066528:CDS 45.0%
!! TTCTGGAGTTCCGAATTTGC+CGG - 32584:5831-5850 MS.gene066528:intergenic 45.0%
!!! CTGGTTTTGGTGATCATAGG+TGG + 32584:6785-6804 MS.gene066528:CDS 45.0%
AATTCTTCGAGAACGCGGTG+AGG - 32584:5908-5927 MS.gene066528:intergenic 50.0%
ACCTTCCCTTTAGGTCCCAT+TGG - 32584:6559-6578 MS.gene066528:intergenic 50.0%
ATATGGCTTTCGAGGTTCCG+TGG + 32584:6392-6411 MS.gene066528:CDS 50.0%
CACGTGAGTAGAGCTGTTGA+CGG - 32584:5871-5890 MS.gene066528:intergenic 50.0%
CTTGATGCTTGGGCTCCAAT+GGG + 32584:6541-6560 MS.gene066528:CDS 50.0%
GGGAAGGTTAGAGATGAAGC+TGG + 32584:6571-6590 MS.gene066528:CDS 50.0%
GGGATGAACAACGTTCATCG+TGG + 32584:6068-6087 MS.gene066528:CDS 50.0%
TATGGCTTTCGAGGTTCCGT+GGG + 32584:6393-6412 MS.gene066528:CDS 50.0%
TCCAATGGGACCTAAAGGGA+AGG + 32584:6555-6574 MS.gene066528:CDS 50.0%
TCTTGATGCTTGGGCTCCAA+TGG + 32584:6540-6559 MS.gene066528:CDS 50.0%
TGGTGATCATAGGTGGCCAT+TGG + 32584:6792-6811 MS.gene066528:CDS 50.0%
AACAGCTCTACTCACGTGGC+CGG + 32584:5873-5892 MS.gene066528:CDS 55.0%
ATCGTCCTCCGTGGCACTAT+CGG + 32584:5803-5822 MS.gene066528:CDS 55.0%
CGTCAACAGCTCTACTCACG+TGG + 32584:5869-5888 MS.gene066528:CDS 55.0%
GCAACTGGGAGGGAACAATG+GGG + 32584:6670-6689 MS.gene066528:CDS 55.0%
GGCAACTGGGAGGGAACAAT+GGG + 32584:6669-6688 MS.gene066528:CDS 55.0%
GGGCTCCAATGGGACCTAAA+GGG + 32584:6551-6570 MS.gene066528:CDS 55.0%
GGTGAGGAAGAGACAGAGGA+AGG - 32584:5780-5799 MS.gene066528:intergenic 55.0%
TGACGGTGAGGAAGAGACAG+AGG - 32584:5784-5803 MS.gene066528:intergenic 55.0%
TGGCAACTGGGAGGGAACAA+TGG + 32584:6668-6687 MS.gene066528:CDS 55.0%
TGGGCTCCAATGGGACCTAA+AGG + 32584:6550-6569 MS.gene066528:CDS 55.0%
! GCTGTTGACGGATCTCGTTG+AGG - 32584:5859-5878 MS.gene066528:intergenic 55.0%
!! ACTATCGGCGCCGGCAAATT+CGG + 32584:5818-5837 MS.gene066528:CDS 55.0%
!! GGTTCCGTGGGAGCGTTATA+AGG + 32584:6405-6424 MS.gene066528:CDS 55.0%
ACTGGGAGGGAACAATGGGG+TGG + 32584:6673-6692 MS.gene066528:CDS 60.0%
AGTGCCACGGAGGACGATGA+CGG - 32584:5801-5820 MS.gene066528:intergenic 60.0%
CTGGGAGGGAACAATGGGGT+GGG + 32584:6674-6693 MS.gene066528:CDS 60.0%
GGAATGCGCGCTGCATCTGA+CGG - 32584:5742-5761 MS.gene066528:intergenic 60.0%
!! AGAGGAAGGTGAGAGTGCCG+CGG - 32584:5766-5785 MS.gene066528:intergenic 60.0%
CGCGCTGCATCTGACGGAGA+CGG - 32584:5736-5755 MS.gene066528:intergenic 65.0%
CTCACCGTCATCGTCCTCCG+TGG + 32584:5794-5813 MS.gene066528:CDS 65.0%
! CACGGAGGACGATGACGGTG+AGG - 32584:5796-5815 MS.gene066528:intergenic 65.0%
ATGCAGCGCGCATTCCGCCG+CGG + 32584:5746-5765 MS.gene066528:CDS 70.0%
CTCCGTGGCACTATCGGCGC+CGG + 32584:5809-5828 MS.gene066528:CDS 70.0%
!! GGAAGGTGAGAGTGCCGCGG+CGG - 32584:5763-5782 MS.gene066528:intergenic 70.0%
!! TGCCGGCGCCGATAGTGCCA+CGG - 32584:5814-5833 MS.gene066528:intergenic 70.0%
CGCGGTGAGGCTCAACGCGC+CGG - 32584:5895-5914 MS.gene066528:intergenic 75.0%
!! CGGCGCCGATAGTGCCACGG+AGG - 32584:5811-5830 MS.gene066528:intergenic 75.0%


Chromosome Type Strat End Strand Name
32584 gene 5707 7071 5707 ID=MS.gene066528
32584 mRNA 5707 7071 5707 ID=MS.gene066528.t1;Parent=MS.gene066528
32584 exon 5707 7071 5707 ID=MS.gene066528.t1.exon1;Parent=MS.gene066528.t1
32584 CDS 5707 7071 5707 ID=cds.MS.gene066528.t1;Parent=MS.gene066528.t1
Gene Sequence

>MS.gene066528

ATGCTGGAGCGGTGTCTCGGATCTCGCCGTCTCCGTCAGATGCAGCGCGCATTCCGCCGCGGCACTCTCACCTTCCTCTGTCTCTTCCTCACCGTCATCGTCCTCCGTGGCACTATCGGCGCCGGCAAATTCGGAACTCCAGAACAAGACCTCAACGAGATCCGTCAACAGCTCTACTCACGTGGCCGGCGCGTTGAGCCTCACCGCGTTCTCGAAGAATTTCAATCCACAGAAAACAACAACGATAACAACAACTACGCCACTTTCGATATCTCGACAATCCTCAAAGATGAAGAAGCTGGTGATGATGAAAAACGCGATCCAAACACGCCTTATACACTTGGTCCCAAAATCTCCGATTGGGATGAACAACGTTCATCGTGGCTGAGTAACAACCCTGATTATCCTAATTTTATTAATCCTAATAAGCCACGTGTTCTTTTAGTTACTGGTTCTTCACCTAAACCGTGTGAAAATCCTGTTGGTGATCATTATTTGTTGAAATCAATTAAGAATAAGATTGATTATTGTAGGCTTCATGGGATTGAGATTTTCTATAACATGGCTTTACTTGATGCTGAAATGGCTGGTTTTTGGGCTAAGCTTCCTTTGATTAGGAAGCTATTGTTGTCGCATCCTGAAGTTGAGTTTTTGTGGTGGATGGATAGTGATGCTATGTTTACTGATATGGCTTTCGAGGTTCCGTGGGAGCGTTATAAGGATCATAATTTTGTGATGCATGGTTGGAATGAGATGATTTATGATGAGAAGAATTGGATTGGTTTGAATACTGGAAGTTTTTTGTTGAGGAATTGTCAGTGGTCACTTGATATTCTTGATGCTTGGGCTCCAATGGGACCTAAAGGGAAGGTTAGAGATGAAGCTGGGAAGATTCTTACTAGGGAGTTAAAGAATCGACCGGTTTTCGAAGCAGATGATCAATCTGCTATGGTTTATTTGTTGGCAACTGGGAGGGAACAATGGGGTGGGAAAGTTTATCTTGAGAATCATTATTATTTGCATGGTTATTGGGGGATTTTGGTTGATAGATATGAAGAGATGATTGAGAATTATCATCCTGGTTTTGGTGATCATAGGTGGCCATTGGTGACTCATTTTGTGGGGTGTAAACCTTGTGGGAAATTTGGTGATTACCCTGTTGAGAGGTGTTTGAAACAAATGGATCGTGCTTATAATTTTGGTGATAATCAGATATTGCAGATGTATGGTTTTACTCATAAGTCACTTGCTAGTAGAAGAGTGAAGAGAGTTAGGAATGAGAGTAGTAATCCTCTTGATGTTAAAGATGAACTTGGATTGCTTCATCCTGCTTTCAAAGCTATCAAGTTGCCAACTTCTTCTTGA

Protein sequence

>MS.gene066528.t1

MLERCLGSRRLRQMQRAFRRGTLTFLCLFLTVIVLRGTIGAGKFGTPEQDLNEIRQQLYSRGRRVEPHRVLEEFQSTENNNDNNNYATFDISTILKDEEAGDDEKRDPNTPYTLGPKISDWDEQRSSWLSNNPDYPNFINPNKPRVLLVTGSSPKPCENPVGDHYLLKSIKNKIDYCRLHGIEIFYNMALLDAEMAGFWAKLPLIRKLLLSHPEVEFLWWMDSDAMFTDMAFEVPWERYKDHNFVMHGWNEMIYDEKNWIGLNTGSFLLRNCQWSLDILDAWAPMGPKGKVRDEAGKILTRELKNRPVFEADDQSAMVYLLATGREQWGGKVYLENHYYLHGYWGILVDRYEEMIENYHPGFGDHRWPLVTHFVGCKPCGKFGDYPVERCLKQMDRAYNFGDNQILQMYGFTHKSLASRRVKRVRNESSNPLDVKDELGLLHPAFKAIKLPTSS