Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06669.t1 | XP_003602287.2 | 99.6 | 228 | 1 | 0 | 30 | 257 | 1 | 228 | 1.10E-129 | 472.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06669.t1 | G7ID19 | 46.7 | 261 | 97 | 14 | 30 | 257 | 1 | 252 | 3.0e-46 | 186.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06669.t1 | G7J7H7 | 99.6 | 228 | 1 | 0 | 30 | 257 | 1 | 228 | 8.2e-130 | 472.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05119 | MS.gene06669 | 0.81232 | 4.50E-51 | -1.69E-46 |
| MS.gene052309 | MS.gene06669 | 0.846244 | 2.45E-59 | -1.69E-46 |
| MS.gene054930 | MS.gene06669 | -0.806199 | 9.24E-50 | -1.69E-46 |
| MS.gene056087 | MS.gene06669 | -0.804235 | 2.38E-49 | -1.69E-46 |
| MS.gene061040 | MS.gene06669 | 0.811029 | 8.59E-51 | -1.69E-46 |
| MS.gene061114 | MS.gene06669 | 0.835659 | 1.48E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06669.t1 | MTR_3g091860 | 99.561 | 228 | 1 | 0 | 30 | 257 | 1 | 228 | 6.55e-171 | 471 |
| MS.gene06669.t1 | MTR_3g091860 | 99.490 | 196 | 1 | 0 | 30 | 225 | 1 | 196 | 2.23e-145 | 407 |
| MS.gene06669.t1 | MTR_1g099190 | 46.743 | 261 | 97 | 14 | 30 | 257 | 1 | 252 | 3.46e-58 | 186 |
| MS.gene06669.t1 | MTR_6g034835 | 44.800 | 250 | 109 | 10 | 30 | 254 | 1 | 246 | 6.55e-56 | 180 |
| MS.gene06669.t1 | MTR_1g099190 | 40.909 | 198 | 77 | 12 | 30 | 196 | 1 | 189 | 2.41e-30 | 112 |
| MS.gene06669.t1 | MTR_3g104670 | 44.776 | 134 | 57 | 3 | 131 | 248 | 90 | 222 | 4.65e-30 | 112 |
| MS.gene06669.t1 | MTR_3g104670 | 43.860 | 114 | 47 | 3 | 131 | 228 | 90 | 202 | 7.68e-23 | 93.2 |
| MS.gene06669.t1 | MTR_1g099190 | 36.413 | 184 | 77 | 12 | 30 | 182 | 1 | 175 | 6.20e-18 | 79.3 |
| MS.gene06669.t1 | MTR_3g104670 | 44.737 | 76 | 26 | 2 | 138 | 197 | 96 | 171 | 4.88e-13 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06669.t1 | AT1G76940 | 43.515 | 239 | 118 | 6 | 30 | 257 | 1 | 233 | 2.39e-52 | 170 |
| MS.gene06669.t1 | AT1G21320 | 40.711 | 253 | 125 | 6 | 30 | 257 | 1 | 253 | 1.41e-50 | 166 |
| MS.gene06669.t1 | AT1G21320 | 43.333 | 210 | 102 | 4 | 65 | 257 | 1 | 210 | 7.83e-46 | 152 |
| MS.gene06669.t1 | AT1G21320 | 43.128 | 211 | 103 | 4 | 64 | 257 | 46 | 256 | 3.25e-45 | 152 |
| MS.gene06669.t1 | AT1G76940 | 43.860 | 228 | 79 | 8 | 30 | 257 | 1 | 179 | 4.92e-45 | 149 |
| MS.gene06669.t1 | AT1G21320 | 43.128 | 211 | 103 | 4 | 64 | 257 | 211 | 421 | 4.96e-44 | 154 |
| MS.gene06669.t1 | AT1G76940 | 34.161 | 161 | 90 | 5 | 30 | 179 | 1 | 156 | 3.17e-15 | 72.0 |
| MS.gene06669.t1 | AT1G21320 | 34.586 | 133 | 70 | 4 | 64 | 179 | 211 | 343 | 2.12e-12 | 66.6 |
Find 86 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCGTCATGTCTGACGCTTAT+TGG | 0.193305 | 3.3:+76685723 | MS.gene06669:CDS |
| ACCGCAACTCGGTCAATTTA+CGG | 0.265379 | 3.3:+76691569 | MS.gene06669:CDS |
| GTCAGTACGGGGACGTTTGC+CGG | 0.281742 | 3.3:-76685786 | None:intergenic |
| TGATTCTCATGTCACAAGTA+TGG | 0.302346 | 3.3:+76690062 | MS.gene06669:CDS |
| CACCACTTAATTCCAGGATT+TGG | 0.309261 | 3.3:-76690116 | None:intergenic |
| GCCTTCCACCACTTAATTCC+AGG | 0.321168 | 3.3:-76690122 | None:intergenic |
| GCAGATTTCATCTCATGGTT+CGG | 0.343063 | 3.3:+76689990 | MS.gene06669:intron |
| CAGATTTCATCTCATGGTTC+GGG | 0.373471 | 3.3:+76689991 | MS.gene06669:intron |
| ACGTTTGCCGGGGATGGTAG+GGG | 0.374467 | 3.3:-76685774 | None:intergenic |
| GGACGTTTGCCGGGGATGGT+AGG | 0.394051 | 3.3:-76685776 | None:intergenic |
| GACGTTTGCCGGGGATGGTA+GGG | 0.408483 | 3.3:-76685775 | None:intergenic |
| TTCCAATTCGCTCGCAACCC+TGG | 0.411050 | 3.3:+76691592 | MS.gene06669:CDS |
| ATTACGGAGGTAACGCTCAT+AGG | 0.414276 | 3.3:-76689454 | None:intergenic |
| GCTCGCAACCCTGGGAGGTC+AGG | 0.419951 | 3.3:+76691601 | MS.gene06669:CDS |
| AGAATCATCAATAGAATGAC+TGG | 0.421510 | 3.3:-76690046 | None:intergenic |
| AACCCTGGGAGGTCAGGTGG+CGG | 0.429538 | 3.3:+76691607 | MS.gene06669:CDS |
| TCAGTACGGGGACGTTTGCC+GGG | 0.438662 | 3.3:-76685785 | None:intergenic |
| TCCCAGGGTTGCGAGCGAAT+TGG | 0.438678 | 3.3:-76691594 | None:intergenic |
| CGTTTGCCGGGGATGGTAGG+GGG | 0.445448 | 3.3:-76685773 | None:intergenic |
| ATTCGGTATCTCTAATTACT+TGG | 0.450640 | 3.3:-76689419 | None:intergenic |
| TGTCTCGGGCGTTCATAACT+TGG | 0.451494 | 3.3:+76689359 | MS.gene06669:CDS |
| ACTTCTTTGTAGCCAACAAA+AGG | 0.466417 | 3.3:-76690321 | None:intergenic |
| GGGAGCGTGCTGCTGAGATT+CGG | 0.469974 | 3.3:-76685753 | None:intergenic |
| TCCAATTCGCTCGCAACCCT+GGG | 0.472195 | 3.3:+76691593 | MS.gene06669:CDS |
| ATGGCAGTTGCTGCATGAGC+CGG | 0.479544 | 3.3:-76690489 | None:intergenic |
| TGCTACCAATACACTATTTG+TGG | 0.482749 | 3.3:+76690167 | MS.gene06669:CDS |
| TTGCTGCAGATTTCATCTCA+TGG | 0.485693 | 3.3:+76689985 | MS.gene06669:intron |
| TGCCCGCCACCTGACCTCCC+AGG | 0.486471 | 3.3:-76691610 | None:intergenic |
| ACCCTCCACAAATAGTGTAT+TGG | 0.494925 | 3.3:-76690172 | None:intergenic |
| CAGTCTACTAGAACCATTGA+TGG | 0.495884 | 3.3:+76690015 | MS.gene06669:CDS |
| ACCCTGGGAGGTCAGGTGGC+GGG | 0.496699 | 3.3:+76691608 | MS.gene06669:CDS |
| TTTCCCCACGACGATGATAG+AGG | 0.497115 | 3.3:+76689390 | MS.gene06669:CDS |
| GATTCTCATGTCACAAGTAT+GGG | 0.497759 | 3.3:+76690063 | MS.gene06669:CDS |
| ATAGGATGCATCAAGGGATT+CGG | 0.501338 | 3.3:-76689436 | None:intergenic |
| ACGGGGACGTTTGCCGGGGA+TGG | 0.501626 | 3.3:-76685780 | None:intergenic |
| CTGAATACCGTAGTCAGTAC+GGG | 0.508975 | 3.3:-76685798 | None:intergenic |
| AAATAATTAATACCGCATTA+CGG | 0.514264 | 3.3:-76689470 | None:intergenic |
| ACTGAATACCGTAGTCAGTA+CGG | 0.515403 | 3.3:-76685799 | None:intergenic |
| AAATGTAAAAGACCAAATCC+TGG | 0.525445 | 3.3:+76690104 | MS.gene06669:CDS |
| CCAGGTGGAAGTGAATGGTC+AGG | 0.532724 | 3.3:-76690144 | None:intergenic |
| CGCAACCCTGGGAGGTCAGG+TGG | 0.536874 | 3.3:+76691604 | MS.gene06669:CDS |
| GCCCGCCACCTGACCTCCCA+GGG | 0.537819 | 3.3:-76691609 | None:intergenic |
| AGCGTCAGACATGACGGTGT+TGG | 0.542130 | 3.3:-76685717 | None:intergenic |
| AGGTCAGGTGGCGGGCATCG+TGG | 0.543236 | 3.3:+76691616 | MS.gene06669:CDS |
| TAGCACCAGGTGGAAGTGAA+TGG | 0.546132 | 3.3:-76690149 | None:intergenic |
| GGCTCATGCAGCAACTGCCA+TGG | 0.546144 | 3.3:+76690491 | MS.gene06669:CDS |
| ATTACCATGCAATGCATCCA+TGG | 0.548719 | 3.3:-76690508 | None:intergenic |
| CCTTCCTCTATCATCGTCGT+GGG | 0.554327 | 3.3:-76689394 | None:intergenic |
| AGTCTACTAGAACCATTGAT+GGG | 0.560461 | 3.3:+76690016 | MS.gene06669:CDS |
| TACTTCGCGTCTTGTACAGT+TGG | 0.562365 | 3.3:-76690202 | None:intergenic |
| GCCTTCCTCTATCATCGTCG+TGG | 0.562600 | 3.3:-76689395 | None:intergenic |
| AAACGTCCCCGTACTGACTA+CGG | 0.563243 | 3.3:+76685791 | MS.gene06669:CDS |
| ACCAATACACTATTTGTGGA+GGG | 0.567944 | 3.3:+76690171 | MS.gene06669:CDS |
| GACCAAATCCTGGAATTAAG+TGG | 0.568245 | 3.3:+76690114 | MS.gene06669:CDS |
| AACGCTCATAGGATGCATCA+AGG | 0.578292 | 3.3:-76689443 | None:intergenic |
| TCGCGTCTTGTACAGTTGGA+AGG | 0.582546 | 3.3:-76690198 | None:intergenic |
| CCTGACCATTCACTTCCACC+TGG | 0.598787 | 3.3:+76690144 | MS.gene06669:CDS |
| TCCTGGAATTAAGTGGTGGA+AGG | 0.599053 | 3.3:+76690121 | MS.gene06669:CDS |
| TGTCAGCAAGGAATCAAGAC+AGG | 0.599053 | 3.3:+76690350 | MS.gene06669:CDS |
| ATTCTCATGTCACAAGTATG+GGG | 0.599732 | 3.3:+76690064 | MS.gene06669:CDS |
| TACCAATACACTATTTGTGG+AGG | 0.606235 | 3.3:+76690170 | MS.gene06669:CDS |
| CCAATAAGCGTCAGACATGA+CGG | 0.612476 | 3.3:-76685723 | None:intergenic |
| AGAAGTTAGACTTGTCAGCA+AGG | 0.615035 | 3.3:+76690338 | MS.gene06669:CDS |
| TGGGATTATGTGCAGCCTGG+GGG | 0.617426 | 3.3:+76690429 | MS.gene06669:intron |
| CAGTACGGGGACGTTTGCCG+GGG | 0.621369 | 3.3:-76685784 | None:intergenic |
| ACCGTAAATTGACCGAGTTG+CGG | 0.624559 | 3.3:-76691570 | None:intergenic |
| TGATGAGCTTGACCGCAACT+CGG | 0.625250 | 3.3:+76691558 | MS.gene06669:CDS |
| CCCACGACGATGATAGAGGA+AGG | 0.628399 | 3.3:+76689394 | MS.gene06669:CDS |
| AGTTAGATTGTGAATACGAA+TGG | 0.632662 | 3.3:-76685675 | None:intergenic |
| TTCTCATGTCACAAGTATGG+GGG | 0.635726 | 3.3:+76690065 | MS.gene06669:CDS |
| GTCTACTAGAACCATTGATG+GGG | 0.636718 | 3.3:+76690017 | MS.gene06669:CDS |
| TCAACAAAACAAAGTAACAG+TGG | 0.640494 | 3.3:-76690456 | None:intergenic |
| ACGCTCATAGGATGCATCAA+GGG | 0.644974 | 3.3:-76689442 | None:intergenic |
| AATAGTGTATTGGTAGCACC+AGG | 0.647665 | 3.3:-76690162 | None:intergenic |
| TGAGCGTTACCTCCGTAATG+CGG | 0.655465 | 3.3:+76689458 | MS.gene06669:CDS |
| AATTCGCTCGCAACCCTGGG+AGG | 0.657729 | 3.3:+76691596 | MS.gene06669:CDS |
| CAAATCCTGGAATTAAGTGG+TGG | 0.659162 | 3.3:+76690117 | MS.gene06669:CDS |
| GAATCATCAATAGAATGACT+GGG | 0.666778 | 3.3:-76690045 | None:intergenic |
| TCTCATGTCACAAGTATGGG+GGG | 0.667031 | 3.3:+76690066 | MS.gene06669:CDS |
| ACTGCCATGGATGCATTGCA+TGG | 0.674544 | 3.3:+76690504 | MS.gene06669:CDS |
| AGTGTATTGGTAGCACCAGG+TGG | 0.684914 | 3.3:-76690159 | None:intergenic |
| CTTCCTCTATCATCGTCGTG+GGG | 0.700696 | 3.3:-76689393 | None:intergenic |
| TCTACTAGAACCATTGATGG+GGG | 0.713611 | 3.3:+76690018 | MS.gene06669:CDS |
| TGTTCGTTCAAGTCTACACG+AGG | 0.716661 | 3.3:-76685650 | None:intergenic |
| TAATTAATACCGCATTACGG+AGG | 0.728809 | 3.3:-76689467 | None:intergenic |
| TGAATACCGTAGTCAGTACG+GGG | 0.745232 | 3.3:-76685797 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 76680877 | 76691648 | 76680877 | ID=MS.gene06669 |
| chr3.3 | mRNA | 76680877 | 76691648 | 76680877 | ID=MS.gene06669.t1;Parent=MS.gene06669 |
| chr3.3 | exon | 76680877 | 76680879 | 76680877 | ID=MS.gene06669.t1.exon1;Parent=MS.gene06669.t1 |
| chr3.3 | CDS | 76680877 | 76680879 | 76680877 | ID=cds.MS.gene06669.t1;Parent=MS.gene06669.t1 |
| chr3.3 | exon | 76685644 | 76685812 | 76685644 | ID=MS.gene06669.t1.exon2;Parent=MS.gene06669.t1 |
| chr3.3 | CDS | 76685644 | 76685812 | 76685644 | ID=cds.MS.gene06669.t1;Parent=MS.gene06669.t1 |
| chr3.3 | exon | 76689358 | 76689479 | 76689358 | ID=MS.gene06669.t1.exon3;Parent=MS.gene06669.t1 |
| chr3.3 | CDS | 76689358 | 76689479 | 76689358 | ID=cds.MS.gene06669.t1;Parent=MS.gene06669.t1 |
| chr3.3 | exon | 76689994 | 76690228 | 76689994 | ID=MS.gene06669.t1.exon4;Parent=MS.gene06669.t1 |
| chr3.3 | CDS | 76689994 | 76690228 | 76689994 | ID=cds.MS.gene06669.t1;Parent=MS.gene06669.t1 |
| chr3.3 | exon | 76690310 | 76690371 | 76690310 | ID=MS.gene06669.t1.exon5;Parent=MS.gene06669.t1 |
| chr3.3 | CDS | 76690310 | 76690371 | 76690310 | ID=cds.MS.gene06669.t1;Parent=MS.gene06669.t1 |
| chr3.3 | exon | 76690444 | 76690525 | 76690444 | ID=MS.gene06669.t1.exon6;Parent=MS.gene06669.t1 |
| chr3.3 | CDS | 76690444 | 76690525 | 76690444 | ID=cds.MS.gene06669.t1;Parent=MS.gene06669.t1 |
| chr3.3 | exon | 76691548 | 76691648 | 76691548 | ID=MS.gene06669.t1.exon7;Parent=MS.gene06669.t1 |
| chr3.3 | CDS | 76691548 | 76691648 | 76691548 | ID=cds.MS.gene06669.t1;Parent=MS.gene06669.t1 |
| Gene Sequence |
| Protein sequence |
