Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067120.t1 | XP_003595584.1 | 95 | 218 | 9 | 1 | 1 | 218 | 31 | 246 | 2.00E-50 | 209.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067120.t1 | O65399 | 52.8 | 89 | 40 | 2 | 130 | 216 | 381 | 469 | 4.5e-19 | 96.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067120.t1 | G7IIX1 | 95.0 | 218 | 9 | 1 | 1 | 218 | 31 | 246 | 1.4e-50 | 209.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049239 | MS.gene067120 | 0.835432 | 1.68E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCATGATGAATTGATATTA+AGG | + | 15190:13857-13876 | MS.gene067120:intergenic | 20.0% |
| !!! | GTTATTTTCTATATTGTTTC+AGG | - | 15190:14059-14078 | MS.gene067120:CDS | 20.0% |
| ! | AATTCATCATGATCATTGAA+CGG | - | 15190:13864-13883 | MS.gene067120:CDS | 25.0% |
| ! | AGATATTCATACAACATTGA+AGG | - | 15190:13831-13850 | MS.gene067120:CDS | 25.0% |
| ! | TACTTGATTTAGATCATGTT+TGG | + | 15190:14031-14050 | MS.gene067120:intergenic | 25.0% |
| ! | TGTTGTATGAATATCTTACT+TGG | + | 15190:13827-13846 | MS.gene067120:intergenic | 25.0% |
| !! | CATGATCTTTCAAAGTATTT+GGG | + | 15190:13715-13734 | MS.gene067120:intergenic | 25.0% |
| ! | GCATGATCTTTCAAAGTATT+TGG | + | 15190:13716-13735 | MS.gene067120:intergenic | 30.0% |
| ! | TCTATATTGTTTCAGGTCAT+GGG | - | 15190:14066-14085 | MS.gene067120:CDS | 30.0% |
| ! | TTCTATATTGTTTCAGGTCA+TGG | - | 15190:14065-14084 | MS.gene067120:CDS | 30.0% |
| ACATGTGTTAATGTTGTGTC+AGG | + | 15190:13924-13943 | MS.gene067120:intergenic | 35.0% | |
| AGCATAAACGTTTGTCATGT+AGG | + | 15190:13986-14005 | MS.gene067120:intergenic | 35.0% | |
| GATTGTTCACAACTTCAACA+AGG | - | 15190:13674-13693 | MS.gene067120:CDS | 35.0% | |
| GTTGGGTTTATAGTAGGATA+AGG | + | 15190:13293-13312 | MS.gene067120:intergenic | 35.0% | |
| TTGGATTATGCTTGTGGATA+TGG | - | 15190:13647-13666 | MS.gene067120:CDS | 35.0% | |
| TTGTTGGAGTTGTTGTAGTT+GGG | + | 15190:13502-13521 | MS.gene067120:intergenic | 35.0% | |
| ! | GACTACTTTAAAGGTAGCTT+TGG | - | 15190:13628-13647 | MS.gene067120:CDS | 35.0% |
| ! | GGTTTGAGTATGGATTTGTT+GGG | + | 15190:13382-13401 | MS.gene067120:intergenic | 35.0% |
| !! | ATGGATTTGTTGAACTTGGT+GGG | + | 15190:13463-13482 | MS.gene067120:intergenic | 35.0% |
| !! | GAGTATGGATTTGTTGAACT+TGG | + | 15190:13467-13486 | MS.gene067120:intergenic | 35.0% |
| !! | TATGGATTTGTTGAACTTGG+TGG | + | 15190:13464-13483 | MS.gene067120:intergenic | 35.0% |
| AATACACAACTTGTAGGTGC+AGG | + | 15190:13773-13792 | MS.gene067120:intergenic | 40.0% | |
| AGCTGCAGAGACTACTTTAA+AGG | - | 15190:13619-13638 | MS.gene067120:CDS | 40.0% | |
| ATGGATTTGTAGAAGCTGGT+GGG | + | 15190:13433-13452 | MS.gene067120:intergenic | 40.0% | |
| CCTACAAGTTGTGTATTTGG+AGG | - | 15190:13776-13795 | MS.gene067120:CDS | 40.0% | |
| CCTCCAAATACACAACTTGT+AGG | + | 15190:13779-13798 | MS.gene067120:intergenic | 40.0% | |
| CTTGTTGGAGTTGTTGTAGT+TGG | + | 15190:13503-13522 | MS.gene067120:intergenic | 40.0% | |
| GAGTATGGATTTGTAGAAGC+TGG | + | 15190:13437-13456 | MS.gene067120:intergenic | 40.0% | |
| GCACCTACAAGTTGTGTATT+TGG | - | 15190:13773-13792 | MS.gene067120:CDS | 40.0% | |
| GGAGATGTTGGGTTTATAGT+AGG | + | 15190:13299-13318 | MS.gene067120:intergenic | 40.0% | |
| GGGTTTGAGTATGGATTTGT+TGG | + | 15190:13383-13402 | MS.gene067120:intergenic | 40.0% | |
| GTTTGAATAGGGACTTGTAG+AGG | + | 15190:13411-13430 | MS.gene067120:intergenic | 40.0% | |
| GTTTGTCCAGTACTTGTATC+AGG | + | 15190:13329-13348 | MS.gene067120:intergenic | 40.0% | |
| TATGGATTTGTAGAAGCTGG+TGG | + | 15190:13434-13453 | MS.gene067120:intergenic | 40.0% | |
| TGTTCACAACTTCAACAAGG+AGG | - | 15190:13677-13696 | MS.gene067120:CDS | 40.0% | |
| ! | TTGTTGTAGTTGGGCTTGTT+GGG | + | 15190:13493-13512 | MS.gene067120:intergenic | 40.0% |
| !! | GTAGCTTTGGATTATGCTTG+TGG | - | 15190:13641-13660 | MS.gene067120:CDS | 40.0% |
| ATTGTGAACCCTACAACACC+AGG | - | 15190:13254-13273 | MS.gene067120:intron | 45.0% | |
| CAACCACCTGATACAAGTAC+TGG | - | 15190:13320-13339 | MS.gene067120:intron | 45.0% | |
| GAATAGGGACTTGTAGAGGT+TGG | + | 15190:13407-13426 | MS.gene067120:intergenic | 45.0% | |
| GATCATTGAACGGTAGTGTC+TGG | - | 15190:13874-13893 | MS.gene067120:CDS | 45.0% | |
| GGTCAAACACTGATACGTGT+CGG | + | 15190:13903-13922 | MS.gene067120:intergenic | 45.0% | |
| GTACTTGTATCAGGTGGTTG+AGG | + | 15190:13320-13339 | MS.gene067120:intergenic | 45.0% | |
| GTGTTGTGGGAGTCACATTT+GGG | + | 15190:13526-13545 | MS.gene067120:intergenic | 45.0% | |
| GTTGTTGTAGTTGGGCTTGT+TGG | + | 15190:13494-13513 | MS.gene067120:intergenic | 45.0% | |
| TAAGGATTTCCTCCTGTTCC+TGG | + | 15190:13275-13294 | MS.gene067120:intergenic | 45.0% | |
| TGTCCAGTACTTGTATCAGG+TGG | + | 15190:13326-13345 | MS.gene067120:intergenic | 45.0% | |
| ! | GGTGTTGTAGGGTTCACAAT+AGG | + | 15190:13254-13273 | MS.gene067120:intergenic | 45.0% |
| !! | AGAAGCTGGTGGGTTTGAAT+AGG | + | 15190:13423-13442 | MS.gene067120:intergenic | 45.0% |
| !! | GAACTTGGTGGGTTTGAGTA+TGG | + | 15190:13452-13471 | MS.gene067120:intergenic | 45.0% |
| !! | GAAGCTGGTGGGTTTGAATA+GGG | + | 15190:13422-13441 | MS.gene067120:intergenic | 45.0% |
| AACCCTACAACACCAGGAAC+AGG | - | 15190:13260-13279 | MS.gene067120:intron | 50.0% | |
| AATGTGACTCCCACAACACC+AGG | - | 15190:13527-13546 | MS.gene067120:CDS | 50.0% | |
| ACACCAGGAACAGGATCATC+TGG | - | 15190:13542-13561 | MS.gene067120:CDS | 50.0% | |
| CAGGTGGTTGAGGAGATGTT+GGG | + | 15190:13310-13329 | MS.gene067120:intergenic | 50.0% | |
| GGAGTCACATTTGGGCTTGT+TGG | + | 15190:13518-13537 | MS.gene067120:intergenic | 50.0% | |
| TAGGGACTTGTAGAGGTTGG+TGG | + | 15190:13404-13423 | MS.gene067120:intergenic | 50.0% | |
| TCAGGTGGTTGAGGAGATGT+TGG | + | 15190:13311-13330 | MS.gene067120:intergenic | 50.0% | |
| ! | AGGGACTTGTAGAGGTTGGT+GGG | + | 15190:13403-13422 | MS.gene067120:intergenic | 50.0% |
| ! | GGTGTTGTGGGAGTCACATT+TGG | + | 15190:13527-13546 | MS.gene067120:intergenic | 50.0% |
| ! | TGATCCTGTTCCTGGTGTTG+TGG | + | 15190:13540-13559 | MS.gene067120:intergenic | 50.0% |
| !! | CTCCTGTTCCTGGTGTTGTA+GGG | + | 15190:13265-13284 | MS.gene067120:intergenic | 50.0% |
| !! | GAGGTTGGTGGGTTTGAGTA+TGG | + | 15190:13392-13411 | MS.gene067120:intergenic | 50.0% |
| !! | GATCCTGTTCCTGGTGTTGT+GGG | + | 15190:13539-13558 | MS.gene067120:intergenic | 50.0% |
| !! | GGGCTTGTTGGGTTTGAGTA+TGG | + | 15190:13482-13501 | MS.gene067120:intergenic | 50.0% |
| ACTCCCACAACACCAGGAAC+AGG | - | 15190:13533-13552 | MS.gene067120:CDS | 55.0% | |
| CCACCAGATGATCCTGTTCC+TGG | + | 15190:13548-13567 | MS.gene067120:intergenic | 55.0% | |
| CCAGGAACAGGATCATCTGG+TGG | - | 15190:13545-13564 | MS.gene067120:CDS | 55.0% | |
| CCTACAACACCAGGAACAGG+AGG | - | 15190:13263-13282 | MS.gene067120:intron | 55.0% | |
| GGAACAGGATCATCTGGTGG+TGG | - | 15190:13548-13567 | MS.gene067120:CDS | 55.0% | |
| !! | ACAGGATCATCTGGTGGTGG+TGG | - | 15190:13551-13570 | MS.gene067120:CDS | 55.0% |
| !! | CCTCCTGTTCCTGGTGTTGT+AGG | + | 15190:13266-13285 | MS.gene067120:intergenic | 55.0% |
| !! | GGCTTGTTGTGCCTGTGTCT+GGG | + | 15190:13361-13380 | MS.gene067120:intergenic | 55.0% |
| GTTGTGCCTGTGTCTGGGCT+TGG | + | 15190:13356-13375 | MS.gene067120:intergenic | 60.0% | |
| ! | TGCCTGTGTCTGGGCTTGGT+GGG | + | 15190:13352-13371 | MS.gene067120:intergenic | 60.0% |
| !! | GGATCATCTGGTGGTGGTGG+TGG | - | 15190:13554-13573 | MS.gene067120:CDS | 60.0% |
| !! | GGGCTTGTTGTGCCTGTGTC+TGG | + | 15190:13362-13381 | MS.gene067120:intergenic | 60.0% |
| !! | GTGGTGGTGGTAGTGGTCAG+TGG | - | 15190:13579-13598 | MS.gene067120:CDS | 60.0% |
| !! | TCATCTGGTGGTGGTGGTGG+TGG | - | 15190:13557-13576 | MS.gene067120:CDS | 60.0% |
| ACCCCACCAAGCCCAGACAC+AGG | - | 15190:13347-13366 | MS.gene067120:intron | 65.0% | |
| GTGCCTGTGTCTGGGCTTGG+TGG | + | 15190:13353-13372 | MS.gene067120:intergenic | 65.0% | |
| !! | GCCTGTGTCTGGGCTTGGTG+GGG | + | 15190:13351-13370 | MS.gene067120:intergenic | 65.0% |
| !! | GGTGGTGGTGGTGGTGGTAG+TGG | - | 15190:13572-13591 | MS.gene067120:CDS | 65.0% |
| !! | TCTGGTGGTGGTGGTGGTGG+TGG | - | 15190:13560-13579 | MS.gene067120:CDS | 65.0% |
| !! | GGTGGTGGTGGTGGTGGTGG+TGG | - | 15190:13563-13582 | MS.gene067120:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 15190 | gene | 13210 | 14120 | 13210 | ID=MS.gene067120 |
| 15190 | mRNA | 13210 | 14120 | 13210 | ID=MS.gene067120.t1;Parent=MS.gene067120 |
| 15190 | exon | 13503 | 14120 | 13503 | ID=MS.gene067120.t1.exon1;Parent=MS.gene067120.t1 |
| 15190 | CDS | 13503 | 14120 | 13503 | ID=cds.MS.gene067120.t1;Parent=MS.gene067120.t1 |
| 15190 | exon | 13210 | 13249 | 13210 | ID=MS.gene067120.t1.exon2;Parent=MS.gene067120.t1 |
| 15190 | CDS | 13210 | 13249 | 13210 | ID=cds.MS.gene067120.t1;Parent=MS.gene067120.t1 |
| Gene Sequence |
| Protein sequence |
