Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067147.t1 | XP_024635368.1 | 82.9 | 41 | 4 | 1 | 19 | 59 | 30 | 67 | 5.50E-07 | 63.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067147.t1 | G7JBQ1 | 82.9 | 41 | 4 | 1 | 19 | 59 | 30 | 67 | 4.0e-07 | 63.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051719 | MS.gene067147 | 0.823594 | 1.27E-53 | -1.69E-46 |
| MS.gene051720 | MS.gene067147 | 0.823004 | 1.75E-53 | -1.69E-46 |
| MS.gene053144 | MS.gene067147 | 0.807615 | 4.64E-50 | -1.69E-46 |
| MS.gene054584 | MS.gene067147 | 0.80617 | 9.37E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067147.t1 | MTR_3g107970 | 82.927 | 41 | 4 | 1 | 19 | 59 | 30 | 67 | 1.14e-13 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGATCAAGGGCAAAGGTTT+TGG | 0.191093 | 3.1:+85642956 | MS.gene067147:CDS |
| TCTCTTCACCTCTTAACTAT+TGG | 0.276120 | 3.1:-85643747 | None:intergenic |
| TCTAACCTACTCTCTTTACT+AGG | 0.352117 | 3.1:-85643714 | None:intergenic |
| AAATTGTAGAATCTCATCTT+AGG | 0.386484 | 3.1:+85643663 | MS.gene067147:intron |
| CAAAACCTTTGCCCTTGATC+TGG | 0.423251 | 3.1:-85642955 | None:intergenic |
| AAGAGCAACAGCTTCCAAAA+AGG | 0.433513 | 3.1:+85643008 | MS.gene067147:CDS |
| AAGATTGTCCAATAGTTAAG+AGG | 0.481585 | 3.1:+85643739 | MS.gene067147:CDS |
| CACTCAGAATTACCAGATCA+AGG | 0.485115 | 3.1:+85642943 | MS.gene067147:CDS |
| ATGTTCCTCACCGTAAAAGA+AGG | 0.498778 | 3.1:+85643070 | MS.gene067147:CDS |
| CCTCACCGTAAAAGAAGGGC+AGG | 0.504738 | 3.1:+85643075 | MS.gene067147:CDS |
| GACCCCTCTTCGGAAAATGA+AGG | 0.532727 | 3.1:+85642982 | MS.gene067147:CDS |
| CTAACCTACTCTCTTTACTA+GGG | 0.584236 | 3.1:-85643713 | None:intergenic |
| ACCCCTCTTCGGAAAATGAA+GGG | 0.596652 | 3.1:+85642983 | MS.gene067147:CDS |
| AATTACCAGATCAAGGGCAA+AGG | 0.620424 | 3.1:+85642950 | MS.gene067147:CDS |
| GAGACCCTAGTAAAGAGAGT+AGG | 0.620938 | 3.1:+85643709 | MS.gene067147:CDS |
| ACTCAGAATTACCAGATCAA+GGG | 0.628344 | 3.1:+85642944 | MS.gene067147:CDS |
| TTGTAGAATCTCATCTTAGG+TGG | 0.633299 | 3.1:+85643666 | MS.gene067147:intron |
| TGTTCCTCACCGTAAAAGAA+GGG | 0.641902 | 3.1:+85643071 | MS.gene067147:CDS |
| CTTGATCTGGTAATTCTGAG+TGG | 0.660376 | 3.1:-85642942 | None:intergenic |
| CTCACCGTAAAAGAAGGGCA+GGG | 0.713304 | 3.1:+85643076 | MS.gene067147:CDS |
| TTAGGTGGTAGAGACATGTG+TGG | 0.716212 | 3.1:+85643681 | MS.gene067147:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATAATTTAAAACATGAATT+TGG | - | chr3.1:85643190-85643209 | None:intergenic | 10.0% |
| !!! | AATTGGTACATTTTATTTTA+TGG | - | chr3.1:85643480-85643499 | None:intergenic | 15.0% |
| !!! | GTAATGAAAATTTATGTTTT+GGG | - | chr3.1:85643586-85643605 | None:intergenic | 15.0% |
| !!! | TGTAATGAAAATTTATGTTT+TGG | - | chr3.1:85643587-85643606 | None:intergenic | 15.0% |
| !! | TATCATATCACCATATAATT+AGG | + | chr3.1:85643208-85643227 | MS.gene067147:intron | 20.0% |
| !! | TATGATAGAATAGAACATTA+TGG | + | chr3.1:85643290-85643309 | MS.gene067147:intron | 20.0% |
| !!! | AAATCTTTCTGATAGTTTTT+AGG | - | chr3.1:85643241-85643260 | None:intergenic | 20.0% |
| !!! | AAGATTTGAAATTAATGAGT+TGG | + | chr3.1:85643254-85643273 | MS.gene067147:intron | 20.0% |
| ! | AAATTGTAGAATCTCATCTT+AGG | + | chr3.1:85643663-85643682 | MS.gene067147:intron | 25.0% |
| ! | ACTATCATTTCCTAATGATA+TGG | - | chr3.1:85643376-85643395 | None:intergenic | 25.0% |
| ! | TAAAATGTACCAATTGAACT+TGG | + | chr3.1:85643485-85643504 | MS.gene067147:intron | 25.0% |
| !! | ATGAATTTGGTGACAAAATT+GGG | - | chr3.1:85643177-85643196 | None:intergenic | 25.0% |
| !! | TGTTAAACAATCCAATTTTC+AGG | + | chr3.1:85643144-85643163 | MS.gene067147:intron | 25.0% |
| !! | TTAATGAGTTGGACTAAATA+TGG | + | chr3.1:85643265-85643284 | MS.gene067147:intron | 25.0% |
| AAGATTGTCCAATAGTTAAG+AGG | + | chr3.1:85643739-85643758 | MS.gene067147:CDS | 30.0% | |
| AATAGATTTGCCTTCAAAGT+TGG | + | chr3.1:85643522-85643541 | MS.gene067147:intron | 30.0% | |
| AGGTCTTCTTCCTAATTATA+TGG | - | chr3.1:85643221-85643240 | None:intergenic | 30.0% | |
| ATAGATTTGCCTTCAAAGTT+GGG | + | chr3.1:85643523-85643542 | MS.gene067147:intron | 30.0% | |
| GATTGATCTATCTAGGTAAT+TGG | + | chr3.1:85643427-85643446 | MS.gene067147:intron | 30.0% | |
| GTTGTTATCACCATATCATT+AGG | + | chr3.1:85643363-85643382 | MS.gene067147:intron | 30.0% | |
| GTTTAACACGTTTATCATGT+GGG | - | chr3.1:85643132-85643151 | None:intergenic | 30.0% | |
| TGGGATTTATACCTGAAAAT+TGG | - | chr3.1:85643158-85643177 | None:intergenic | 30.0% | |
| TGTTTAACACGTTTATCATG+TGG | - | chr3.1:85643133-85643152 | None:intergenic | 30.0% | |
| ! | CATGAATTTGGTGACAAAAT+TGG | - | chr3.1:85643178-85643197 | None:intergenic | 30.0% |
| !!! | AAGCTTTTTTCAACTAAGTG+GGG | - | chr3.1:85643339-85643358 | None:intergenic | 30.0% |
| !!! | CAAGCTTTTTTCAACTAAGT+GGG | - | chr3.1:85643340-85643359 | None:intergenic | 30.0% |
| ACATGCAGATTGATCTATCT+AGG | + | chr3.1:85643420-85643439 | MS.gene067147:intron | 35.0% | |
| ACTCAGAATTACCAGATCAA+GGG | + | chr3.1:85642944-85642963 | MS.gene067147:CDS | 35.0% | |
| ACTTACATACCCAACTTTGA+AGG | - | chr3.1:85643535-85643554 | None:intergenic | 35.0% | |
| ATTATGGCGTAATTTGATCC+TGG | + | chr3.1:85643306-85643325 | MS.gene067147:intron | 35.0% | |
| CCACTTAGTTGAAAAAAGCT+TGG | + | chr3.1:85643338-85643357 | MS.gene067147:intron | 35.0% | |
| CTAACCTACTCTCTTTACTA+GGG | - | chr3.1:85643716-85643735 | None:intergenic | 35.0% | |
| TCTAACCTACTCTCTTTACT+AGG | - | chr3.1:85643717-85643736 | None:intergenic | 35.0% | |
| TTGTAGAATCTCATCTTAGG+TGG | + | chr3.1:85643666-85643685 | MS.gene067147:intron | 35.0% | |
| ! | CTCAGAAGAAACAACCTTTT+TGG | - | chr3.1:85643025-85643044 | None:intergenic | 35.0% |
| !!! | CCAAGCTTTTTTCAACTAAG+TGG | - | chr3.1:85643341-85643360 | None:intergenic | 35.0% |
| !!! | TTTTTTCAACTAAGTGGGGT+CGG | - | chr3.1:85643335-85643354 | None:intergenic | 35.0% |
| AAGAGCAACAGCTTCCAAAA+AGG | + | chr3.1:85643008-85643027 | MS.gene067147:CDS | 40.0% | |
| AAGTCAGCTGAAATTGCTAG+AGG | + | chr3.1:85643551-85643570 | MS.gene067147:intron | 40.0% | |
| AATTACCAGATCAAGGGCAA+AGG | + | chr3.1:85642950-85642969 | MS.gene067147:CDS | 40.0% | |
| AGTCAGCTGAAATTGCTAGA+GGG | + | chr3.1:85643552-85643571 | MS.gene067147:intron | 40.0% | |
| ATGTTCCTCACCGTAAAAGA+AGG | + | chr3.1:85643070-85643089 | MS.gene067147:CDS | 40.0% | |
| CACTCAGAATTACCAGATCA+AGG | + | chr3.1:85642943-85642962 | MS.gene067147:CDS | 40.0% | |
| CTTGATCTGGTAATTCTGAG+TGG | - | chr3.1:85642945-85642964 | None:intergenic | 40.0% | |
| TGTTCCTCACCGTAAAAGAA+GGG | + | chr3.1:85643071-85643090 | MS.gene067147:CDS | 40.0% | |
| TTTGCTCGACCAAGTTCAAT+TGG | - | chr3.1:85643497-85643516 | None:intergenic | 40.0% | |
| ! | AGATCAAGGGCAAAGGTTTT+GGG | + | chr3.1:85642957-85642976 | MS.gene067147:CDS | 40.0% |
| ! | TTCCCTTCATTTTCCGAAGA+GGG | - | chr3.1:85642988-85643007 | None:intergenic | 40.0% |
| ! | TTTCCCTTCATTTTCCGAAG+AGG | - | chr3.1:85642989-85643008 | None:intergenic | 40.0% |
| ACCCCTCTTCGGAAAATGAA+GGG | + | chr3.1:85642983-85643002 | MS.gene067147:CDS | 45.0% | |
| CAAAACCTTTGCCCTTGATC+TGG | - | chr3.1:85642958-85642977 | None:intergenic | 45.0% | |
| GAGACCCTAGTAAAGAGAGT+AGG | + | chr3.1:85643709-85643728 | MS.gene067147:CDS | 45.0% | |
| GTCAGCTGAAATTGCTAGAG+GGG | + | chr3.1:85643553-85643572 | MS.gene067147:intron | 45.0% | |
| TTAGGTGGTAGAGACATGTG+TGG | + | chr3.1:85643681-85643700 | MS.gene067147:CDS | 45.0% | |
| ! | CAGATCAAGGGCAAAGGTTT+TGG | + | chr3.1:85642956-85642975 | MS.gene067147:CDS | 45.0% |
| ! | CTTTTACGGTGAGGAACATC+AGG | - | chr3.1:85643069-85643088 | None:intergenic | 45.0% |
| ! | TAAGCCCTGCCCTTCTTTTA+CGG | - | chr3.1:85643083-85643102 | None:intergenic | 45.0% |
| ! | TCCCTTCATTTTCCGAAGAG+GGG | - | chr3.1:85642987-85643006 | None:intergenic | 45.0% |
| CTCACCGTAAAAGAAGGGCA+GGG | + | chr3.1:85643076-85643095 | MS.gene067147:CDS | 50.0% | |
| GACCCCTCTTCGGAAAATGA+AGG | + | chr3.1:85642982-85643001 | MS.gene067147:CDS | 50.0% | |
| !!! | TCAAGGGCAAAGGTTTTGGG+AGG | + | chr3.1:85642960-85642979 | MS.gene067147:CDS | 50.0% |
| CCTCACCGTAAAAGAAGGGC+AGG | + | chr3.1:85643075-85643094 | MS.gene067147:CDS | 55.0% | |
| ! | CCTGCCCTTCTTTTACGGTG+AGG | - | chr3.1:85643078-85643097 | None:intergenic | 55.0% |
| !!! | GTTTTGGGAGGACCCCTCTT+CGG | + | chr3.1:85642972-85642991 | MS.gene067147:CDS | 55.0% |
| ACTAAGTGGGGTCGGCTACC+AGG | - | chr3.1:85643327-85643346 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 85642931 | 85643764 | 85642931 | ID=MS.gene067147 |
| chr3.1 | mRNA | 85642931 | 85643764 | 85642931 | ID=MS.gene067147.t1;Parent=MS.gene067147 |
| chr3.1 | exon | 85642931 | 85643107 | 85642931 | ID=MS.gene067147.t1.exon1;Parent=MS.gene067147.t1 |
| chr3.1 | CDS | 85642931 | 85643107 | 85642931 | ID=cds.MS.gene067147.t1;Parent=MS.gene067147.t1 |
| chr3.1 | exon | 85643672 | 85643764 | 85643672 | ID=MS.gene067147.t1.exon2;Parent=MS.gene067147.t1 |
| chr3.1 | CDS | 85643672 | 85643764 | 85643672 | ID=cds.MS.gene067147.t1;Parent=MS.gene067147.t1 |
| Gene Sequence |
| Protein sequence |
