AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene067381

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067381.t1 MTR_5g073960 78.155 412 89 1 6 417 5 415 0.0 654
MS.gene067381.t1 MTR_5g074130 49.341 683 316 10 1 659 1 677 0.0 604
MS.gene067381.t1 MTR_5g073920 48.665 674 307 10 4 656 3 658 0.0 587
MS.gene067381.t1 MTR_5g073460 48.052 693 318 12 6 659 5 694 0.0 584
MS.gene067381.t1 MTR_3g464010 47.353 680 314 13 6 657 5 668 0.0 565
MS.gene067381.t1 MTR_5g073480 45.723 678 279 14 6 651 5 625 9.07e-177 517
MS.gene067381.t1 MTR_5g073900 70.225 356 91 2 270 625 1 341 5.82e-173 497
MS.gene067381.t1 MTR_5g073620 47.636 571 235 8 5 525 4 560 4.48e-152 468
MS.gene067381.t1 MTR_5g073620 41.700 247 122 5 419 656 834 1067 1.07e-41 163
MS.gene067381.t1 MTR_5g073880 70.849 271 58 3 1 270 1 251 4.40e-126 374
MS.gene067381.t1 MTR_5g073450 45.618 445 205 9 243 655 192 631 1.07e-105 334
MS.gene067381.t1 MTR_5g073810 43.400 447 190 10 6 424 5 416 6.97e-99 310
MS.gene067381.t1 MTR_3g031130 44.889 450 203 13 244 659 183 621 3.60e-96 309
MS.gene067381.t1 MTR_5g074190 57.143 280 66 4 380 658 184 410 4.02e-95 302
MS.gene067381.t1 MTR_5g074190 79.032 186 36 1 6 191 2 184 1.55e-92 296
MS.gene067381.t1 MTR_5g093530 62.869 237 85 2 1 237 1 234 3.85e-89 289
MS.gene067381.t1 MTR_5g093530 40.510 353 179 4 316 659 244 574 2.39e-70 239
MS.gene067381.t1 MTR_5g073610 47.205 322 152 8 6 319 5 316 5.81e-82 262
MS.gene067381.t1 MTR_5g074450 55.422 249 91 2 4 232 3 251 9.78e-81 265
MS.gene067381.t1 MTR_5g073770 41.109 433 173 8 6 416 5 377 7.44e-80 259
MS.gene067381.t1 MTR_5g073750 49.818 275 133 4 6 276 5 278 3.01e-72 235
MS.gene067381.t1 MTR_5g073470 47.195 303 108 5 6 308 5 255 5.34e-72 234
MS.gene067381.t1 MTR_5g073840 58.019 212 82 4 3 210 2 210 2.09e-69 227
MS.gene067381.t1 MTR_5g073820 35.417 528 195 16 11 516 29 432 2.65e-67 228
MS.gene067381.t1 MTR_5g074090 62.500 160 57 2 14 171 13 171 2.18e-59 199
MS.gene067381.t1 MTR_5g074150 62.025 158 60 0 14 171 24 181 5.27e-57 194
MS.gene067381.t1 MTR_5g074200 46.154 234 119 3 7 237 3 232 6.15e-54 188
MS.gene067381.t1 MTR_5g073860 66.423 137 42 2 8 144 21 153 4.51e-52 184
MS.gene067381.t1 MTR_5g073650 36.857 350 159 9 103 425 1 315 5.67e-50 177
MS.gene067381.t1 MTR_8g021330 28.667 450 268 11 252 655 161 603 4.32e-48 179
MS.gene067381.t1 MTR_4g103540 29.385 439 273 11 249 655 245 678 9.68e-48 179
MS.gene067381.t1 MTR_6g033310 29.438 445 260 10 257 655 287 723 5.57e-46 174
MS.gene067381.t1 MTR_7g026630 29.571 443 261 13 256 655 214 648 1.07e-45 172
MS.gene067381.t1 MTR_5g073660 40.569 281 95 6 11 261 29 267 1.40e-45 164
MS.gene067381.t1 MTR_5g073780 41.631 233 115 4 433 656 2 222 2.15e-45 161
MS.gene067381.t1 MTR_1g057560 28.472 432 268 10 255 655 202 623 3.94e-45 171
MS.gene067381.t1 MTR_1g090620 27.891 441 269 11 255 655 208 639 6.50e-45 170
MS.gene067381.t1 MTR_5g074070 52.532 158 53 3 3 159 2 138 5.60e-39 144
MS.gene067381.t1 MTR_7g013770 27.655 452 268 16 251 655 225 664 8.18e-39 153
MS.gene067381.t1 MTR_6g078770 41.232 211 122 2 19 228 109 318 7.31e-37 141
MS.gene067381.t1 MTR_6g078610 38.760 258 123 7 5 229 580 835 7.17e-36 145
MS.gene067381.t1 MTR_1g117090 26.338 467 273 12 258 656 204 667 5.87e-32 132
MS.gene067381.t1 MTR_2g096230 24.887 442 279 12 253 659 158 581 8.65e-32 131
MS.gene067381.t1 MTR_6g075870 41.212 165 95 2 11 173 605 769 1.02e-30 129
MS.gene067381.t1 MTR_5g093540 45.098 153 58 2 7 159 3 129 3.46e-30 119
MS.gene067381.t1 MTR_5g073500 45.946 148 49 2 14 161 1 117 4.64e-30 115
MS.gene067381.t1 MTR_4g062330 24.569 464 273 12 244 647 155 601 5.42e-30 125
MS.gene067381.t1 MTR_1g067460 26.846 447 273 17 252 655 164 599 1.33e-29 124
MS.gene067381.t1 MTR_3g025900 25.290 431 276 12 252 648 181 599 5.08e-29 122
MS.gene067381.t1 MTR_1g067480 26.949 449 269 18 252 655 166 600 3.66e-28 120
MS.gene067381.t1 MTR_4g023690 72.222 72 19 1 71 142 16 86 1.08e-27 107
MS.gene067381.t1 MTR_5g073830 61.905 84 31 1 4 86 3 86 9.57e-25 103
MS.gene067381.t1 MTR_5g073490 38.182 165 76 4 502 655 3 152 2.43e-24 100
MS.gene067381.t1 MTR_4g023660 57.143 84 35 1 219 302 1 83 9.08e-24 96.3
MS.gene067381.t1 MTR_6g078620 37.870 169 70 3 11 176 84 220 1.09e-21 94.7
MS.gene067381.t1 MTR_6g078770 37.179 156 66 2 19 173 109 233 7.06e-20 91.7
MS.gene067381.t1 MTR_4g023700 50.667 75 36 1 219 293 1 74 1.67e-17 77.8
MS.gene067381.t1 MTR_4g024790 50.602 83 23 2 423 505 1 65 9.77e-15 69.7
MS.gene067381.t1 MTR_5g073850 37.391 115 68 2 86 199 13 124 3.53e-14 71.2
MS.gene067381.t1 MTR_1g009620 21.399 486 303 16 226 651 181 647 7.40e-14 75.5
MS.gene067381.t1 MTR_7g017290 70.455 44 13 0 571 614 46 89 7.81e-13 65.1
MS.gene067381.t1 MTR_7g017290 65.217 46 15 1 448 493 1 45 1.37e-11 61.6
MS.gene067381.t1 MTR_6g078630 59.259 54 22 0 16 69 201 254 2.35e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067381.t1 AT5G58430 43.349 436 214 9 245 655 189 616 3.42e-101 322
MS.gene067381.t1 AT1G07000 36.986 438 235 11 245 655 171 594 1.09e-75 254
MS.gene067381.t1 AT1G54090 28.894 443 259 11 252 648 170 602 4.84e-51 187
MS.gene067381.t1 AT3G14090 26.697 442 269 11 252 648 173 604 6.11e-49 181
MS.gene067381.t1 AT1G72470 29.268 451 263 11 252 655 178 619 1.43e-48 181
MS.gene067381.t1 AT5G13150 30.159 441 257 11 258 655 210 642 3.86e-47 177
MS.gene067381.t1 AT5G50380 27.955 440 276 11 252 655 235 669 4.36e-46 174
MS.gene067381.t1 AT5G03540 28.018 439 266 12 255 655 87 513 2.72e-45 169
MS.gene067381.t1 AT5G03540 28.018 439 266 12 255 655 228 654 6.42e-45 171
MS.gene067381.t1 AT5G03540 28.571 441 261 12 255 655 202 628 6.62e-45 170
MS.gene067381.t1 AT5G52340 28.904 429 268 9 255 655 202 621 2.64e-43 165
MS.gene067381.t1 AT5G13990 29.195 435 261 12 256 655 257 679 1.81e-41 161
MS.gene067381.t1 AT5G52350 26.573 429 278 10 255 655 156 575 4.30e-34 138
MS.gene067381.t1 AT3G29400 25.322 466 275 13 249 655 194 645 5.24e-34 138
MS.gene067381.t1 AT1G07725 25.400 437 273 12 258 659 175 593 8.08e-31 128
MS.gene067381.t1 AT2G39380 26.253 419 244 13 258 624 170 575 1.48e-28 121
MS.gene067381.t1 AT2G28640 26.477 457 286 12 240 659 137 580 1.08e-27 119
MS.gene067381.t1 AT5G59730 25.558 403 248 13 258 618 165 557 8.99e-26 113
MS.gene067381.t1 AT5G59730 25.558 403 248 13 258 618 165 557 1.27e-25 112
MS.gene067381.t1 AT3G09530 24.401 459 283 16 252 659 159 604 1.49e-25 112
MS.gene067381.t1 AT3G09520 26.134 463 285 18 245 659 155 608 3.40e-24 108
MS.gene067381.t1 AT5G61010 23.168 423 274 14 267 655 219 624 1.79e-23 105
MS.gene067381.t1 AT5G61010 23.168 423 274 14 267 655 219 624 1.79e-23 105
MS.gene067381.t1 AT2G28650 25.700 393 250 9 263 627 152 530 1.76e-22 102
MS.gene067381.t1 AT3G55150 25.285 439 265 15 263 655 187 608 2.78e-22 102
MS.gene067381.t1 AT4G31540 22.888 367 261 8 312 658 293 657 3.72e-14 76.6
MS.gene067381.t1 AT1G51640 24.269 342 228 8 312 625 253 591 8.47e-12 68.9

Find 86 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGAATCTGCTTTAGAATT+TGG 0.139042 5.2:-18254654 None:intergenic
TCAACCATCTATTTGGAAAT+TGG 0.148000 5.2:+18253065 MS.gene067381:CDS
AAACGTTTCGAAGATGTTTA+TGG 0.159868 5.2:+18253627 MS.gene067381:CDS
GCATCAGCCACCTCTGAATT+TGG 0.162332 5.2:-18252967 None:intergenic
TCTTCCTTCAACCATCTATT+TGG 0.201070 5.2:+18253058 MS.gene067381:CDS
AGTACCAACTTGAAACCTTT+TGG 0.261690 5.2:+18254523 MS.gene067381:CDS
TTGTTAATAAATAAATTATT+AGG 0.264803 5.2:+18253826 MS.gene067381:CDS
CATATGAAAACTTCATTGTT+AGG 0.278473 5.2:+18254805 MS.gene067381:CDS
CCCAGCATCCACCATCAATT+TGG 0.290817 5.2:-18253746 None:intergenic
AAGATGGATTAAAGCTTCTA+AGG 0.301067 5.2:+18253885 MS.gene067381:CDS
AATTCCAATTTCCAAATAGA+TGG 0.301868 5.2:-18253069 None:intergenic
AGTAAACAAGGATTCAAATT+CGG 0.329353 5.2:-18254124 None:intergenic
ATGAAGATTAGTTTGTTGTT+AGG 0.331531 5.2:+18253394 MS.gene067381:CDS
GCTACTGTCTTATCATTATT+CGG 0.332438 5.2:+18253437 MS.gene067381:CDS
GATGAAAGGTAACTATAAAT+TGG 0.333450 5.2:+18253654 MS.gene067381:CDS
AGCCACCGGCTACATCGTTT+TGG 0.351952 5.2:+18254299 MS.gene067381:CDS
GTAAACAAGGATTCAAATTC+GGG 0.365812 5.2:-18254123 None:intergenic
GGATTTCAGGATTACATGAT+TGG 0.384710 5.2:+18253862 MS.gene067381:CDS
AGTTTGTTGTTAGGTATTGT+TGG 0.387999 5.2:+18253403 MS.gene067381:CDS
TTCTCTGATAAAATTATGAA+TGG 0.400575 5.2:+18253238 MS.gene067381:CDS
TCGGACGAGTATATAGAGTA+TGG 0.402590 5.2:+18254852 MS.gene067381:CDS
TTCACAGATAGAGCTTATAA+TGG 0.404307 5.2:+18254407 MS.gene067381:CDS
GATAAGACAGTAGCTGTAAC+AGG 0.405810 5.2:-18253428 None:intergenic
GGATTTGCTTCATCTGTTGT+TGG 0.417556 5.2:+18253013 MS.gene067381:CDS
TAACAAAACCGCCAAATTGA+TGG 0.422701 5.2:+18253735 MS.gene067381:CDS
GCCAAATTGATGGTGGATGC+TGG 0.432137 5.2:+18253745 MS.gene067381:CDS
CGATGCAGAGTTGGATTGTT+GGG 0.442610 5.2:+18254245 MS.gene067381:CDS
GAGGTGGCTGATGCATAAAA+AGG 0.445395 5.2:+18252976 MS.gene067381:CDS
AAGCACTGAAATTCAAGAAT+AGG 0.445504 5.2:+18254163 MS.gene067381:CDS
ATCCTAGATAAAAGTACATA+AGG 0.447221 5.2:-18253354 None:intergenic
AGATAATTCAGAGTTTGATT+CGG 0.448867 5.2:-18253962 None:intergenic
TACCCTTTGGTTGTTGCTGA+TGG 0.458174 5.2:+18254363 MS.gene067381:CDS
AATCTTACAAGAATACCCTT+TGG 0.462105 5.2:+18254350 MS.gene067381:CDS
CTGAAATTCAAGAATAGGTT+GGG 0.462376 5.2:+18254168 MS.gene067381:CDS
AGGATTACATGATTGGAAGA+TGG 0.476310 5.2:+18253869 MS.gene067381:CDS
ACAAAATCCATCATCCCAAA+AGG 0.477678 5.2:-18254538 None:intergenic
CCGATGCAGAGTTGGATTGT+TGG 0.483382 5.2:+18254244 MS.gene067381:CDS
ATCCTTGTTTACTGATTCGT+TGG 0.486501 5.2:+18254134 MS.gene067381:CDS
ATTTCCAAATAGATGGTTGA+AGG 0.486838 5.2:-18253062 None:intergenic
AGGCGATTGATTGTTTCATC+CGG 0.488161 5.2:-18253712 None:intergenic
CAACAATCCAACTCTGCATC+GGG 0.493910 5.2:-18254243 None:intergenic
GGTGGATGCTGGGTTTGATA+AGG 0.498506 5.2:+18253756 MS.gene067381:CDS
AGCATCCACCATCAATTTGG+CGG 0.498592 5.2:-18253743 None:intergenic
GTTAGGTTGCATGATGTTCA+CGG 0.499776 5.2:+18254822 MS.gene067381:CDS
TCTTCGATTCAAAGCTCATT+CGG 0.500565 5.2:+18253177 MS.gene067381:CDS
ACATTAGTTTGCGTAAAGAA+TGG 0.500641 5.2:+18253794 MS.gene067381:CDS
TTGTAGAGTCGCCGTTCACT+TGG 0.503028 5.2:-18253928 None:intergenic
CAACGACACGAAAATACAAA+TGG 0.503737 5.2:+18253544 MS.gene067381:CDS
TTGGATTGTTGGGCTGATGG+TGG 0.506236 5.2:+18254255 MS.gene067381:CDS
GAGTTGGATTGTTGGGCTGA+TGG 0.509673 5.2:+18254252 MS.gene067381:CDS
CTTAGAAAACATGTTGCTGC+CGG 0.511486 5.2:+18254782 MS.gene067381:CDS
TTGGTTGTTGCTGATGGAGT+AGG 0.521302 5.2:+18254369 MS.gene067381:CDS
ACTGAAATTCAAGAATAGGT+TGG 0.527255 5.2:+18254167 MS.gene067381:CDS
AAATACTATTTCCAAGTGAA+CGG 0.536396 5.2:+18253917 MS.gene067381:CDS
CAGAAGTTTCGGGAGATATG+TGG 0.544254 5.2:+18254702 MS.gene067381:CDS
CTCCATCAGCAACAACCAAA+GGG 0.550645 5.2:-18254365 None:intergenic
TAGTTTGCGTAAAGAATGGT+TGG 0.552491 5.2:+18253798 MS.gene067381:CDS
TCTAACACCCGATGCAGAGT+TGG 0.555038 5.2:+18254236 MS.gene067381:CDS
CCAACAATCCAACTCTGCAT+CGG 0.555872 5.2:-18254244 None:intergenic
ACGCAAGAAACTCAGTGTCT+TGG 0.557695 5.2:+18253484 MS.gene067381:CDS
CCAAATTGATGGTGGATGCT+GGG 0.558898 5.2:+18253746 MS.gene067381:CDS
TTTCTGGTCAAGGAAGAACT+TGG 0.559149 5.2:+18254323 MS.gene067381:CDS
TTCTAAAGCAGATTCAATGA+AGG 0.559551 5.2:+18254659 MS.gene067381:CDS
CTTCATAATTGATTCCGTGC+CGG 0.562815 5.2:+18253693 MS.gene067381:CDS
AATTCAGTGTGGATTTGAAG+TGG 0.567441 5.2:+18253330 MS.gene067381:CDS
TTAAACAAACGCCACGCTGA+AGG 0.567785 5.2:-18254063 None:intergenic
GAAAGGTAACTATAAATTGG+TGG 0.572426 5.2:+18253657 MS.gene067381:CDS
CCGGTGGCTTCACATGTCAT+AGG 0.574627 5.2:-18254285 None:intergenic
ACTCCATCAGCAACAACCAA+AGG 0.577154 5.2:-18254366 None:intergenic
GAAAGCCAAAACGATGTAGC+CGG 0.589042 5.2:-18254304 None:intergenic
CTCTCATCCAAATTCAGAGG+TGG 0.592010 5.2:+18252960 MS.gene067381:CDS
CATCCCAAAAGGTTTCAAGT+TGG 0.593566 5.2:-18254527 None:intergenic
ATGTTTATGGAGAAGATGAA+AGG 0.596560 5.2:+18253640 MS.gene067381:CDS
CCTCTGAATTTGGATGAGAG+TGG 0.599690 5.2:-18252957 None:intergenic
CAAAACCGCCAAATTGATGG+TGG 0.610738 5.2:+18253738 MS.gene067381:CDS
ATTGATTGTTTCATCCGGCA+CGG 0.620872 5.2:-18253707 None:intergenic
AACCAACGAATCAGTAAACA+AGG 0.627549 5.2:-18254136 None:intergenic
CCTATGACATGTGAAGCCAC+CGG 0.631734 5.2:+18254285 MS.gene067381:CDS
CCACTCTCATCCAAATTCAG+AGG 0.632190 5.2:+18252957 MS.gene067381:CDS
CGACACGAAAATACAAATGG+TGG 0.639692 5.2:+18253547 MS.gene067381:CDS
GCTAAGTGACATGATCGCAA+AGG 0.670694 5.2:-18253296 None:intergenic
AGCCAAAACGATGTAGCCGG+TGG 0.678386 5.2:-18254301 None:intergenic
TATGTGGAATTCAGTCAACA+TGG 0.701076 5.2:+18254718 MS.gene067381:CDS
AGCTAAGTCACAAATATACG+AGG 0.707978 5.2:+18254452 MS.gene067381:CDS
TGAATCAAAGTCCTTCAGCG+TGG 0.717777 5.2:+18254052 MS.gene067381:CDS
TTGTCGAGACAAATTCAGTG+TGG 0.737166 5.2:+18253319 MS.gene067381:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCTCTGATAAAATTATGAA+TGG + chr5.2:18253238-18253257 MS.gene067381:CDS 20.0%
!!! ATTATTAGGTTTTAGAAAGA+TGG + chr5.2:18253840-18253859 MS.gene067381:CDS 20.0%
!!! CATAATTTTATCAGAGAAAA+AGG - chr5.2:18253236-18253255 None:intergenic 20.0%
!!! TTATTAGGTTTTAGAAAGAT+GGG + chr5.2:18253841-18253860 MS.gene067381:CDS 20.0%
! AAATACTATTTCCAAGTGAA+CGG + chr5.2:18253917-18253936 MS.gene067381:CDS 25.0%
! AATTCCAATTTCCAAATAGA+TGG - chr5.2:18253072-18253091 None:intergenic 25.0%
! AGATAATTCAGAGTTTGATT+CGG - chr5.2:18253965-18253984 None:intergenic 25.0%
! AGTAAACAAGGATTCAAATT+CGG - chr5.2:18254127-18254146 None:intergenic 25.0%
! CATATGAAAACTTCATTGTT+AGG + chr5.2:18254805-18254824 MS.gene067381:CDS 25.0%
! GATGAAAGGTAACTATAAAT+TGG + chr5.2:18253654-18253673 MS.gene067381:CDS 25.0%
!! AGAAACATTTTCATGGAATT+TGG + chr5.2:18254201-18254220 MS.gene067381:CDS 25.0%
!! AGTATGTAGAAACATTTTCA+TGG + chr5.2:18254194-18254213 MS.gene067381:CDS 25.0%
!! ATCCTAGATAAAAGTACATA+AGG - chr5.2:18253357-18253376 None:intergenic 25.0%
!! GAAACATTTTCATGGAATTT+GGG + chr5.2:18254202-18254221 MS.gene067381:CDS 25.0%
!!! ATCTGATCTTTGTTTTTTAG+AGG + chr5.2:18253981-18254000 MS.gene067381:CDS 25.0%
!!! ATGAAGATTAGTTTGTTGTT+AGG + chr5.2:18253394-18253413 MS.gene067381:CDS 25.0%
!!! ATTGAATCTGCTTTAGAATT+TGG - chr5.2:18254657-18254676 None:intergenic 25.0%
!!! GGAATTTTTTCAGACAAATT+TGG + chr5.2:18254428-18254447 MS.gene067381:CDS 25.0%
!!! GTTTTTTCAATCAGAAGTTT+CGG + chr5.2:18254691-18254710 MS.gene067381:CDS 25.0%
!!! TGATGGATTTTGTAAAAACA+AGG + chr5.2:18254548-18254567 MS.gene067381:CDS 25.0%
!!! TTTTTTCAATCAGAAGTTTC+GGG + chr5.2:18254692-18254711 MS.gene067381:CDS 25.0%
AAACGTTTCGAAGATGTTTA+TGG + chr5.2:18253627-18253646 MS.gene067381:CDS 30.0%
AATCTTACAAGAATACCCTT+TGG + chr5.2:18254350-18254369 MS.gene067381:CDS 30.0%
ACTGAAATTCAAGAATAGGT+TGG + chr5.2:18254167-18254186 MS.gene067381:CDS 30.0%
ATCCTCTTAACAAAAACAAC+TGG - chr5.2:18254905-18254924 None:intergenic 30.0%
ATGAAAAAATAACGCAAAGC+AGG - chr5.2:18254480-18254499 None:intergenic 30.0%
ATGTTTATGGAGAAGATGAA+AGG + chr5.2:18253640-18253659 MS.gene067381:CDS 30.0%
ATTTCCAAATAGATGGTTGA+AGG - chr5.2:18253065-18253084 None:intergenic 30.0%
CTGAAATTCAAGAATAGGTT+GGG + chr5.2:18254168-18254187 MS.gene067381:CDS 30.0%
GAAAAAAGACTATATGCATC+TGG - chr5.2:18253268-18253287 None:intergenic 30.0%
GAAAGGTAACTATAAATTGG+TGG + chr5.2:18253657-18253676 MS.gene067381:CDS 30.0%
GCTACTGTCTTATCATTATT+CGG + chr5.2:18253437-18253456 MS.gene067381:CDS 30.0%
GTAAACAAGGATTCAAATTC+GGG - chr5.2:18254126-18254145 None:intergenic 30.0%
TCAACCATCTATTTGGAAAT+TGG + chr5.2:18253065-18253084 MS.gene067381:CDS 30.0%
TGAAAAAATAACGCAAAGCA+GGG - chr5.2:18254479-18254498 None:intergenic 30.0%
TGATGTTCACGGTAAAAATT+CGG + chr5.2:18254833-18254852 MS.gene067381:CDS 30.0%
TTCACAGATAGAGCTTATAA+TGG + chr5.2:18254407-18254426 MS.gene067381:CDS 30.0%
! AAAAAGGTATGGAGATTTGT+TGG + chr5.2:18252992-18253011 MS.gene067381:CDS 30.0%
! ACAATGAAGTTTTCATATGC+CGG - chr5.2:18254804-18254823 None:intergenic 30.0%
! GACCTTATGTACTTTTATCT+AGG + chr5.2:18253352-18253371 MS.gene067381:CDS 30.0%
!! AACTCTATTTTAGAAGCTCA+TGG + chr5.2:18254583-18254602 MS.gene067381:CDS 30.0%
!! AAGATGGATTAAAGCTTCTA+AGG + chr5.2:18253885-18253904 MS.gene067381:CDS 30.0%
!! AAGCACTGAAATTCAAGAAT+AGG + chr5.2:18254163-18254182 MS.gene067381:CDS 30.0%
!! ACATTAGTTTGCGTAAAGAA+TGG + chr5.2:18253794-18253813 MS.gene067381:CDS 30.0%
!! AGTTTGTTGTTAGGTATTGT+TGG + chr5.2:18253403-18253422 MS.gene067381:CDS 30.0%
!! TTCTAAAGCAGATTCAATGA+AGG + chr5.2:18254659-18254678 MS.gene067381:CDS 30.0%
!! TTTTAGAAAGATGGGATTTC+AGG + chr5.2:18253849-18253868 MS.gene067381:CDS 30.0%
!! TTTTAGAAGCTCATGGAATA+AGG + chr5.2:18254590-18254609 MS.gene067381:CDS 30.0%
!!! AACCAGTTGTTTTTGTTAAG+AGG + chr5.2:18254900-18254919 MS.gene067381:CDS 30.0%
!!! TGTTTTTTAGAGGTTTGTGA+TGG + chr5.2:18253991-18254010 MS.gene067381:CDS 30.0%
AACCAACGAATCAGTAAACA+AGG - chr5.2:18254139-18254158 None:intergenic 35.0%
AATTCAGTGTGGATTTGAAG+TGG + chr5.2:18253330-18253349 MS.gene067381:CDS 35.0%
ACAAAATCCATCATCCCAAA+AGG - chr5.2:18254541-18254560 None:intergenic 35.0%
AGCTAAGTCACAAATATACG+AGG + chr5.2:18254452-18254471 MS.gene067381:CDS 35.0%
AGGATTACATGATTGGAAGA+TGG + chr5.2:18253869-18253888 MS.gene067381:CDS 35.0%
CAACGACACGAAAATACAAA+TGG + chr5.2:18253544-18253563 MS.gene067381:CDS 35.0%
GGATTTCAGGATTACATGAT+TGG + chr5.2:18253862-18253881 MS.gene067381:CDS 35.0%
TAACAAAACCGCCAAATTGA+TGG + chr5.2:18253735-18253754 MS.gene067381:CDS 35.0%
TATGTGGAATTCAGTCAACA+TGG + chr5.2:18254718-18254737 MS.gene067381:CDS 35.0%
TCTTCCTTCAACCATCTATT+TGG + chr5.2:18253058-18253077 MS.gene067381:CDS 35.0%
TCTTCGATTCAAAGCTCATT+CGG + chr5.2:18253177-18253196 MS.gene067381:CDS 35.0%
! AGTACCAACTTGAAACCTTT+TGG + chr5.2:18254523-18254542 MS.gene067381:CDS 35.0%
! GTACCAACTTGAAACCTTTT+GGG + chr5.2:18254524-18254543 MS.gene067381:CDS 35.0%
! TAGTTTGCGTAAAGAATGGT+TGG + chr5.2:18253798-18253817 MS.gene067381:CDS 35.0%
! TGCTTTTGTATGCAACAACA+TGG + chr5.2:18253140-18253159 MS.gene067381:CDS 35.0%
!! ATCCTTGTTTACTGATTCGT+TGG + chr5.2:18254134-18254153 MS.gene067381:CDS 35.0%
!!! TCAATTTGGCGGTTTTGTTA+AGG - chr5.2:18253735-18253754 None:intergenic 35.0%
AGGCGATTGATTGTTTCATC+CGG - chr5.2:18253715-18253734 None:intergenic 40.0%
ATTGATTGTTTCATCCGGCA+CGG - chr5.2:18253710-18253729 None:intergenic 40.0%
CGACACGAAAATACAAATGG+TGG + chr5.2:18253547-18253566 MS.gene067381:CDS 40.0%
CTTAGAAAACATGTTGCTGC+CGG + chr5.2:18254782-18254801 MS.gene067381:CDS 40.0%
GATAAGACAGTAGCTGTAAC+AGG - chr5.2:18253431-18253450 None:intergenic 40.0%
GGCTGATGCATAAAAAGGTA+TGG + chr5.2:18252981-18253000 MS.gene067381:CDS 40.0%
TCGGACGAGTATATAGAGTA+TGG + chr5.2:18254852-18254871 MS.gene067381:CDS 40.0%
TTGTCGAGACAAATTCAGTG+TGG + chr5.2:18253319-18253338 MS.gene067381:CDS 40.0%
! CAAAGCTCATTCGGCATTTT+TGG + chr5.2:18253186-18253205 MS.gene067381:CDS 40.0%
! CATCCCAAAAGGTTTCAAGT+TGG - chr5.2:18254530-18254549 None:intergenic 40.0%
! CTTCATAATTGATTCCGTGC+CGG + chr5.2:18253693-18253712 MS.gene067381:CDS 40.0%
! GGATTTGCTTCATCTGTTGT+TGG + chr5.2:18253013-18253032 MS.gene067381:CDS 40.0%
! GTTAGGTTGCATGATGTTCA+CGG + chr5.2:18254822-18254841 MS.gene067381:CDS 40.0%
! TTTCTGGTCAAGGAAGAACT+TGG + chr5.2:18254323-18254342 MS.gene067381:CDS 40.0%
!! GTGTTTTTGACGAGCAACTA+AGG + chr5.2:18254742-18254761 MS.gene067381:CDS 40.0%
!!! CTTGAAACCTTTTGGGATGA+TGG + chr5.2:18254531-18254550 MS.gene067381:CDS 40.0%
ACGCAAGAAACTCAGTGTCT+TGG + chr5.2:18253484-18253503 MS.gene067381:CDS 45.0%
ACTCCATCAGCAACAACCAA+AGG - chr5.2:18254369-18254388 None:intergenic 45.0%
AGCATCCACCATCAATTTGG+CGG - chr5.2:18253746-18253765 None:intergenic 45.0%
CAAAACCGCCAAATTGATGG+TGG + chr5.2:18253738-18253757 MS.gene067381:CDS 45.0%
CAACAATCCAACTCTGCATC+GGG - chr5.2:18254246-18254265 None:intergenic 45.0%
CAGAAGTTTCGGGAGATATG+TGG + chr5.2:18254702-18254721 MS.gene067381:CDS 45.0%
CCAACAATCCAACTCTGCAT+CGG - chr5.2:18254247-18254266 None:intergenic 45.0%
CCACTCTCATCCAAATTCAG+AGG + chr5.2:18252957-18252976 MS.gene067381:CDS 45.0%
CCTCTGAATTTGGATGAGAG+TGG - chr5.2:18252960-18252979 None:intergenic 45.0%
CGATGCAGAGTTGGATTGTT+GGG + chr5.2:18254245-18254264 MS.gene067381:CDS 45.0%
CTCCATCAGCAACAACCAAA+GGG - chr5.2:18254368-18254387 None:intergenic 45.0%
CTCTCATCCAAATTCAGAGG+TGG + chr5.2:18252960-18252979 MS.gene067381:CDS 45.0%
GAAAGCCAAAACGATGTAGC+CGG - chr5.2:18254307-18254326 None:intergenic 45.0%
GAGGTGGCTGATGCATAAAA+AGG + chr5.2:18252976-18252995 MS.gene067381:CDS 45.0%
GCTAAGTGACATGATCGCAA+AGG - chr5.2:18253299-18253318 None:intergenic 45.0%
TACCCTTTGGTTGTTGCTGA+TGG + chr5.2:18254363-18254382 MS.gene067381:CDS 45.0%
TGAATCAAAGTCCTTCAGCG+TGG + chr5.2:18254052-18254071 MS.gene067381:CDS 45.0%
TTAAACAAACGCCACGCTGA+AGG - chr5.2:18254066-18254085 None:intergenic 45.0%
TTGGTTGTTGCTGATGGAGT+AGG + chr5.2:18254369-18254388 MS.gene067381:CDS 45.0%
! CGGCAGCAACATGTTTTCTA+AGG - chr5.2:18254784-18254803 None:intergenic 45.0%
!! CCAAATTGATGGTGGATGCT+GGG + chr5.2:18253746-18253765 MS.gene067381:CDS 45.0%
!!! GCTACATCGTTTTGGCTTTC+TGG + chr5.2:18254307-18254326 MS.gene067381:CDS 45.0%
!!! TCGTTTTGGCTTTCTGGTCA+AGG + chr5.2:18254313-18254332 MS.gene067381:CDS 45.0%
!! TTGTTAATAAATAAATTATT+AGG + chr5.2:18253826-18253845 MS.gene067381:CDS 5.0%
CCCAGCATCCACCATCAATT+TGG - chr5.2:18253749-18253768 None:intergenic 50.0%
CCGATGCAGAGTTGGATTGT+TGG + chr5.2:18254244-18254263 MS.gene067381:CDS 50.0%
CCTATGACATGTGAAGCCAC+CGG + chr5.2:18254285-18254304 MS.gene067381:CDS 50.0%
GAGTTGGATTGTTGGGCTGA+TGG + chr5.2:18254252-18254271 MS.gene067381:CDS 50.0%
GCATCAGCCACCTCTGAATT+TGG - chr5.2:18252970-18252989 None:intergenic 50.0%
GGTGGATGCTGGGTTTGATA+AGG + chr5.2:18253756-18253775 MS.gene067381:CDS 50.0%
TCTAACACCCGATGCAGAGT+TGG + chr5.2:18254236-18254255 MS.gene067381:CDS 50.0%
TTGGATTGTTGGGCTGATGG+TGG + chr5.2:18254255-18254274 MS.gene067381:CDS 50.0%
TTGTAGAGTCGCCGTTCACT+TGG - chr5.2:18253931-18253950 None:intergenic 50.0%
!! GCCAAATTGATGGTGGATGC+TGG + chr5.2:18253745-18253764 MS.gene067381:CDS 50.0%
AGCCAAAACGATGTAGCCGG+TGG - chr5.2:18254304-18254323 None:intergenic 55.0%
CCGGTGGCTTCACATGTCAT+AGG - chr5.2:18254288-18254307 None:intergenic 55.0%
!! AGCCACCGGCTACATCGTTT+TGG + chr5.2:18254299-18254318 MS.gene067381:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 18252947 18254929 18252947 ID=MS.gene067381
chr5.2 mRNA 18252947 18254929 18252947 ID=MS.gene067381.t1;Parent=MS.gene067381
chr5.2 exon 18252947 18254929 18252947 ID=MS.gene067381.t1.exon1;Parent=MS.gene067381.t1
chr5.2 CDS 18252947 18254929 18252947 ID=cds.MS.gene067381.t1;Parent=MS.gene067381.t1
Gene Sequence

>MS.gene067381

ATGAAAGAAGCCACTCTCATCCAAATTCAGAGGTGGCTGATGCATAAAAAGGTATGGAGATTTGTTGGATTTGCTTCATCTGTTGTTGGATTGCTTTGTTATGCTCTAAGCTCTTCCTTCAACCATCTATTTGGAAATTGGAATTTGTTGAAGATATTTCTTTACACAGTTTTCAGTTTCATCATTTGTCTAATGCTTTTGTATGCAACAACATGGCAACACTCGACAAGTCTTCGATTCAAAGCTCATTCGGCATTTTTGGTATTGACAATTACTTCTGTCTATTCCTTTTTCTCTGATAAAATTATGAATGGAAAACCAGATGCATATAGTCTTTTTTCATGTGCTGCCTTTGCGATCATGTCACTTAGCTTGTCGAGACAAATTCAGTGTGGATTTGAAGTGGACCTTATGTACTTTTATCTAGGATGTTTAATCGTACAACTCATGAAGATTAGTTTGTTGTTAGGTATTGTTGGAGCCTGTTACAGCTACTGTCTTATCATTATTCGGTCTTCTTTCTCTTCGTTAATTGTTACGCAAGAAACTCAGTGTCTTGGACTCGAAGAACAACGAGTAGTCATTCAAGTTGATTCACAACGACACGAAAATACAAATGGTGGTAGCAGCATCATGCAACAATTTATGACTTGTATGAATGATCTTAAGAAGAACGATTCAAACGTTTCGAAGATGTTTATGGAGAAGATGAAAGGTAACTATAAATTGGTGGTGACTGATCACAACTTCATAATTGATTCCGTGCCGGATGAAACAATCAATCGCCTTAACAAAACCGCCAAATTGATGGTGGATGCTGGGTTTGATAAGGAGTGCTTTGACTTGTACATTAGTTTGCGTAAAGAATGGTTGGAAGATTTGTTAATAAATAAATTATTAGGTTTTAGAAAGATGGGATTTCAGGATTACATGATTGGAAGATGGATTAAAGCTTCTAAGGTTGCTCTTAAAATACTATTTCCAAGTGAACGGCGACTCTACAATTGTGTCTTCTCCGAATCAAACTCTGAATTATCTGATCTTTGTTTTTTAGAGGTTTGTGATGGAGCGACGTGTCAACTTTTGAATTTTGCAGATTCATTTGTGAATCAAAGTCCTTCAGCGTGGCGTTTGTTTAAAATTCTTGACTTGTTTGAGACTATGTGTGATCTCATTCCCGAATTTGAATCCTTGTTTACTGATTCGTTGGTTAACAAAGCACTGAAATTCAAGAATAGGTTGGGTGAAGTATGTAGAAACATTTTCATGGAATTTGGGAAATTGATATTTCTAACACCCGATGCAGAGTTGGATTGTTGGGCTGATGGTGGAGTTCATCCTATGACATGTGAAGCCACCGGCTACATCGTTTTGGCTTTCTGGTCAAGGAAGAACTTGGAGAAAATCTTACAAGAATACCCTTTGGTTGTTGCTGATGGAGTAGGAACATCTTTATTCTATTCACAGATAGAGCTTATAATGGAATTTTTTCAGACAAATTTGGAAGCTAAGTCACAAATATACGAGGACCCTGCTTTGCGTTATTTTTTCATGATGAATAATCTAAGTCACATTAAGTACCAACTTGAAACCTTTTGGGATGATGGATTTTGTAAAAACAAGGAGCAATATTGTGAACTCTATTTTAGAAGCTCATGGAATAAGGTGATAGATTTTTTGAAGATTGATATCGACGAGTCAATGACGCCAAATTCTAAAGCAGATTCAATGAAGGACAATCTCAGTTTTTTCAATCAGAAGTTTCGGGAGATATGTGGAATTCAGTCAACATGGCGTGTTTTTGACGAGCAACTAAGGAAACAAATAATAATTTCCTTAGAAAACATGTTGCTGCCGGCATATGAAAACTTCATTGTTAGGTTGCATGATGTTCACGGTAAAAATTCGGACGAGTATATAGAGTATGGAATGTCTGATATTCAAGATCGACTAAACCAGTTGTTTTTGTTAAGAGGATGTTGA
Protein sequence

>MS.gene067381.t1

MKEATLIQIQRWLMHKKVWRFVGFASSVVGLLCYALSSSFNHLFGNWNLLKIFLYTVFSFIICLMLLYATTWQHSTSLRFKAHSAFLVLTITSVYSFFSDKIMNGKPDAYSLFSCAAFAIMSLSLSRQIQCGFEVDLMYFYLGCLIVQLMKISLLLGIVGACYSYCLIIIRSSFSSLIVTQETQCLGLEEQRVVIQVDSQRHENTNGGSSIMQQFMTCMNDLKKNDSNVSKMFMEKMKGNYKLVVTDHNFIIDSVPDETINRLNKTAKLMVDAGFDKECFDLYISLRKEWLEDLLINKLLGFRKMGFQDYMIGRWIKASKVALKILFPSERRLYNCVFSESNSELSDLCFLEVCDGATCQLLNFADSFVNQSPSAWRLFKILDLFETMCDLIPEFESLFTDSLVNKALKFKNRLGEVCRNIFMEFGKLIFLTPDAELDCWADGGVHPMTCEATGYIVLAFWSRKNLEKILQEYPLVVADGVGTSLFYSQIELIMEFFQTNLEAKSQIYEDPALRYFFMMNNLSHIKYQLETFWDDGFCKNKEQYCELYFRSSWNKVIDFLKIDIDESMTPNSKADSMKDNLSFFNQKFREICGIQSTWRVFDEQLRKQIIISLENMLLPAYENFIVRLHDVHGKNSDEYIEYGMSDIQDRLNQLFLLRGC