Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067432.t1 | XP_003597940.1 | 98.1 | 210 | 3 | 1 | 1 | 210 | 1 | 209 | 4.50E-100 | 374 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067432.t1 | G7IIA4 | 98.1 | 210 | 3 | 1 | 1 | 210 | 1 | 209 | 3.3e-100 | 374.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052011 | MS.gene067432 | -0.800844 | 1.19E-48 | -1.69E-46 |
| MS.gene055217 | MS.gene067432 | 0.808533 | 2.96E-50 | -1.69E-46 |
| MS.gene059247 | MS.gene067432 | 0.805006 | 1.64E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067432.t1 | MTR_2g104230 | 98.095 | 210 | 3 | 1 | 1 | 210 | 1 | 209 | 3.64e-155 | 428 |
| MS.gene067432.t1 | MTR_4g009760 | 60.181 | 221 | 64 | 10 | 1 | 210 | 1 | 208 | 1.57e-81 | 241 |
| MS.gene067432.t1 | MTR_4g116200 | 45.912 | 159 | 70 | 3 | 1 | 158 | 1 | 144 | 2.73e-34 | 122 |
| MS.gene067432.t1 | MTR_2g450720 | 38.919 | 185 | 85 | 6 | 1 | 165 | 1 | 177 | 9.69e-30 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067432.t1 | AT1G15760 | 44.444 | 225 | 79 | 8 | 3 | 209 | 5 | 201 | 1.04e-57 | 181 |
| MS.gene067432.t1 | AT1G80520 | 47.489 | 219 | 77 | 8 | 1 | 209 | 1 | 191 | 1.04e-50 | 163 |
| MS.gene067432.t1 | AT2G12462 | 29.231 | 260 | 121 | 7 | 1 | 209 | 1 | 248 | 4.89e-26 | 101 |
Find 41 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTAGGCCTAATGGTGGATT+TGG | 0.285518 | 2.4:-1410648 | MS.gene067432:CDS |
| GCTTTGTCTGGTGATCTTGA+TGG | 0.316091 | 2.4:-1410510 | MS.gene067432:CDS |
| GAGAATTTCTAATCTGTGTT+TGG | 0.325351 | 2.4:+1410812 | None:intergenic |
| TAAGTGGGATACCATGTTTA+AGG | 0.337355 | 2.4:-1410367 | MS.gene067432:CDS |
| GAGAAAATGTTTCTCACAAA+TGG | 0.339341 | 2.4:-1410567 | MS.gene067432:CDS |
| CCATCTCTTGTATATGAGTA+TGG | 0.354378 | 2.4:-1410921 | MS.gene067432:CDS |
| TACTGAAGAATCAGCACTTA+TGG | 0.357191 | 2.4:-1410679 | MS.gene067432:CDS |
| AGTACTTAGCTAACTATGTT+AGG | 0.383843 | 2.4:-1410713 | MS.gene067432:CDS |
| TTCTAATCTGTGTTTGGCTA+TGG | 0.404684 | 2.4:+1410818 | None:intergenic |
| ATAATCATGATGATGGTTAT+TGG | 0.406535 | 2.4:-1410410 | MS.gene067432:CDS |
| CTAGGCCTAATGGTGGATTT+GGG | 0.437057 | 2.4:-1410647 | MS.gene067432:CDS |
| ACAGTAGCTCCTGCTTTGTC+TGG | 0.454059 | 2.4:-1410522 | MS.gene067432:CDS |
| CACTTATGGTGATGCCAACT+AGG | 0.468970 | 2.4:-1410665 | MS.gene067432:CDS |
| GAAGAGGAACACACAATTTG+TGG | 0.477404 | 2.4:-1410601 | MS.gene067432:CDS |
| CCATGCTTTGTAGAAACTCA+TGG | 0.497469 | 2.4:+1410847 | None:intergenic |
| TTAACTCAACAGCCATGGAT+TGG | 0.500177 | 2.4:-1410974 | None:intergenic |
| TTTCCAAAAGGAGCAAAAGA+TGG | 0.506781 | 2.4:-1410463 | MS.gene067432:CDS |
| AATCCACCATTAGGCCTAGT+TGG | 0.510240 | 2.4:+1410651 | None:intergenic |
| ATGGTGGATTTGGGAGAAGA+TGG | 0.518326 | 2.4:-1410638 | MS.gene067432:CDS |
| CCATGAGTTTCTACAAAGCA+TGG | 0.527981 | 2.4:-1410847 | MS.gene067432:CDS |
| CAAAAGATGGATGGAGATGA+TGG | 0.529408 | 2.4:-1410450 | MS.gene067432:CDS |
| TACAAGAGATGGCTCAAGGT+TGG | 0.536104 | 2.4:+1410932 | None:intergenic |
| CTGCTGTTGAAGAGATTAAG+TGG | 0.538740 | 2.4:-1410383 | MS.gene067432:CDS |
| CTTCTCCCAAATCCACCATT+AGG | 0.539738 | 2.4:+1410642 | None:intergenic |
| AATTTGTAGCTGCAATCAAA+AGG | 0.542395 | 2.4:-1410743 | MS.gene067432:CDS |
| GTGATGCCAACTAGGCCTAA+TGG | 0.547391 | 2.4:-1410657 | MS.gene067432:CDS |
| GATGGAGATGATGGTGGTGA+TGG | 0.550508 | 2.4:-1410441 | MS.gene067432:CDS |
| TGTTGTGTATCATTTCCAAA+AGG | 0.557274 | 2.4:-1410475 | MS.gene067432:CDS |
| CAAAAGGAGCAAAAGATGGA+TGG | 0.561894 | 2.4:-1410459 | MS.gene067432:CDS |
| AATAATGATAATCATGATGA+TGG | 0.562055 | 2.4:-1410417 | MS.gene067432:CDS |
| CCATACTCATATACAAGAGA+TGG | 0.573633 | 2.4:+1410921 | None:intergenic |
| TCAAGATCACCAGACAAAGC+AGG | 0.592387 | 2.4:+1410513 | None:intergenic |
| CATGAGTTTCTACAAAGCAT+GGG | 0.601787 | 2.4:-1410846 | MS.gene067432:CDS |
| GGAAGAGTGCTATAATGAAG+AGG | 0.605141 | 2.4:-1410617 | MS.gene067432:CDS |
| AAATGTTTCTCACAAATGGA+AGG | 0.609404 | 2.4:-1410563 | MS.gene067432:CDS |
| CATATACAAGAGATGGCTCA+AGG | 0.614307 | 2.4:+1410928 | None:intergenic |
| TAGCCATGAAAACCAATCCA+TGG | 0.623251 | 2.4:+1410962 | None:intergenic |
| TGCTGTTGAAGAGATTAAGT+GGG | 0.633418 | 2.4:-1410382 | MS.gene067432:CDS |
| TGGCTTTAGATCCTTAAACA+TGG | 0.634954 | 2.4:+1410356 | None:intergenic |
| ATGCCAACTAGGCCTAATGG+TGG | 0.656564 | 2.4:-1410654 | MS.gene067432:CDS |
| AAGATGGATGGAGATGATGG+TGG | 0.707845 | 2.4:-1410447 | MS.gene067432:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATGATAATCATGATGA+TGG | - | chr2.4:1410893-1410912 | MS.gene067432:CDS | 20.0% |
| !!! | ATAATCATGATGATGGTTAT+TGG | - | chr2.4:1410900-1410919 | MS.gene067432:CDS | 25.0% |
| AAATGTTTCTCACAAATGGA+AGG | - | chr2.4:1410747-1410766 | MS.gene067432:CDS | 30.0% | |
| AATTTGTAGCTGCAATCAAA+AGG | - | chr2.4:1410567-1410586 | MS.gene067432:CDS | 30.0% | |
| AGTACTTAGCTAACTATGTT+AGG | - | chr2.4:1410597-1410616 | MS.gene067432:CDS | 30.0% | |
| GAGAAAATGTTTCTCACAAA+TGG | - | chr2.4:1410743-1410762 | MS.gene067432:CDS | 30.0% | |
| GAGAATTTCTAATCTGTGTT+TGG | + | chr2.4:1410501-1410520 | None:intergenic | 30.0% | |
| TGTTGTGTATCATTTCCAAA+AGG | - | chr2.4:1410835-1410854 | MS.gene067432:CDS | 30.0% | |
| !! | TTTTGGAAATGATACACAAC+AGG | + | chr2.4:1410836-1410855 | None:intergenic | 30.0% |
| CATGAGTTTCTACAAAGCAT+GGG | - | chr2.4:1410464-1410483 | MS.gene067432:CDS | 35.0% | |
| CCATACTCATATACAAGAGA+TGG | + | chr2.4:1410392-1410411 | None:intergenic | 35.0% | |
| CCATCTCTTGTATATGAGTA+TGG | - | chr2.4:1410389-1410408 | MS.gene067432:CDS | 35.0% | |
| TAAGTGGGATACCATGTTTA+AGG | - | chr2.4:1410943-1410962 | MS.gene067432:CDS | 35.0% | |
| TACTGAAGAATCAGCACTTA+TGG | - | chr2.4:1410631-1410650 | MS.gene067432:CDS | 35.0% | |
| TGCTGTTGAAGAGATTAAGT+GGG | - | chr2.4:1410928-1410947 | MS.gene067432:CDS | 35.0% | |
| TTCTAATCTGTGTTTGGCTA+TGG | + | chr2.4:1410495-1410514 | None:intergenic | 35.0% | |
| TTTCCAAAAGGAGCAAAAGA+TGG | - | chr2.4:1410847-1410866 | MS.gene067432:CDS | 35.0% | |
| ! | ACAAATTTTGCAACTGTGTG+TGG | + | chr2.4:1410554-1410573 | None:intergenic | 35.0% |
| !! | TGGCTTTAGATCCTTAAACA+TGG | + | chr2.4:1410957-1410976 | None:intergenic | 35.0% |
| CAAAAGATGGATGGAGATGA+TGG | - | chr2.4:1410860-1410879 | MS.gene067432:CDS | 40.0% | |
| CAAAAGGAGCAAAAGATGGA+TGG | - | chr2.4:1410851-1410870 | MS.gene067432:CDS | 40.0% | |
| CATATACAAGAGATGGCTCA+AGG | + | chr2.4:1410385-1410404 | None:intergenic | 40.0% | |
| CCATGAGTTTCTACAAAGCA+TGG | - | chr2.4:1410463-1410482 | MS.gene067432:CDS | 40.0% | |
| CCATGCTTTGTAGAAACTCA+TGG | + | chr2.4:1410466-1410485 | None:intergenic | 40.0% | |
| CTGCTGTTGAAGAGATTAAG+TGG | - | chr2.4:1410927-1410946 | MS.gene067432:CDS | 40.0% | |
| GAAGAGGAACACACAATTTG+TGG | - | chr2.4:1410709-1410728 | MS.gene067432:CDS | 40.0% | |
| ! | AAAGCAGGAGCTACTGTTTT+TGG | + | chr2.4:1410785-1410804 | None:intergenic | 40.0% |
| ! | CATCCATCTTTTGCTCCTTT+TGG | + | chr2.4:1410853-1410872 | None:intergenic | 40.0% |
| ! | GCTCATTTTGTGCAAAGACA+AGG | + | chr2.4:1410415-1410434 | None:intergenic | 40.0% |
| !! | GGAAGAGTGCTATAATGAAG+AGG | - | chr2.4:1410693-1410712 | MS.gene067432:CDS | 40.0% |
| AATCCACCATTAGGCCTAGT+TGG | + | chr2.4:1410662-1410681 | None:intergenic | 45.0% | |
| CTTCTCCCAAATCCACCATT+AGG | + | chr2.4:1410671-1410690 | None:intergenic | 45.0% | |
| GCTTTGTCTGGTGATCTTGA+TGG | - | chr2.4:1410800-1410819 | MS.gene067432:CDS | 45.0% | |
| TACAAGAGATGGCTCAAGGT+TGG | + | chr2.4:1410381-1410400 | None:intergenic | 45.0% | |
| TCAAGATCACCAGACAAAGC+AGG | + | chr2.4:1410800-1410819 | None:intergenic | 45.0% | |
| ! | AAGATGGATGGAGATGATGG+TGG | - | chr2.4:1410863-1410882 | MS.gene067432:CDS | 45.0% |
| ! | CACTTATGGTGATGCCAACT+AGG | - | chr2.4:1410645-1410664 | MS.gene067432:CDS | 45.0% |
| !! | ACTAGGCCTAATGGTGGATT+TGG | - | chr2.4:1410662-1410681 | MS.gene067432:CDS | 45.0% |
| !! | ATGGTGGATTTGGGAGAAGA+TGG | - | chr2.4:1410672-1410691 | MS.gene067432:CDS | 45.0% |
| !! | CTAGGCCTAATGGTGGATTT+GGG | - | chr2.4:1410663-1410682 | MS.gene067432:CDS | 45.0% |
| !!! | AGGAGCTACTGTTTTTGGCA+TGG | + | chr2.4:1410780-1410799 | None:intergenic | 45.0% |
| ACAGTAGCTCCTGCTTTGTC+TGG | - | chr2.4:1410788-1410807 | MS.gene067432:CDS | 50.0% | |
| ATGCCAACTAGGCCTAATGG+TGG | - | chr2.4:1410656-1410675 | MS.gene067432:CDS | 50.0% | |
| GTGATGCCAACTAGGCCTAA+TGG | - | chr2.4:1410653-1410672 | MS.gene067432:CDS | 50.0% | |
| !! | GATGGAGATGATGGTGGTGA+TGG | - | chr2.4:1410869-1410888 | MS.gene067432:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 1410350 | 1410982 | 1410350 | ID=MS.gene067432 |
| chr2.4 | mRNA | 1410350 | 1410982 | 1410350 | ID=MS.gene067432.t1;Parent=MS.gene067432 |
| chr2.4 | exon | 1410350 | 1410982 | 1410350 | ID=MS.gene067432.t1.exon1;Parent=MS.gene067432.t1 |
| chr2.4 | CDS | 1410350 | 1410982 | 1410350 | ID=cds.MS.gene067432.t1;Parent=MS.gene067432.t1 |
| Gene Sequence |
| Protein sequence |
