Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067526.t1 | XP_013459273.1 | 90.5 | 84 | 8 | 0 | 20 | 103 | 114 | 197 | 3.10E-33 | 151 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067526.t1 | A0A072UTZ0 | 90.5 | 84 | 8 | 0 | 20 | 103 | 114 | 197 | 2.2e-33 | 151.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058037 | MS.gene067526 | 0.8017 | 7.96E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067526.t1 | MTR_3g435250 | 82.979 | 94 | 14 | 1 | 10 | 103 | 106 | 197 | 5.01e-50 | 157 |
| MS.gene067526.t1 | MTR_3g435230 | 72.340 | 94 | 24 | 1 | 10 | 103 | 106 | 197 | 4.64e-42 | 136 |
| MS.gene067526.t1 | MTR_3g435200 | 67.857 | 84 | 27 | 0 | 20 | 103 | 112 | 195 | 2.34e-37 | 124 |
| MS.gene067526.t1 | MTR_3g435170 | 76.389 | 72 | 17 | 0 | 20 | 91 | 114 | 185 | 5.79e-35 | 119 |
| MS.gene067526.t1 | MTR_5g041930 | 54.762 | 84 | 38 | 0 | 20 | 103 | 119 | 202 | 2.23e-30 | 107 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067526.t1 | AT1G18720 | 60.465 | 86 | 34 | 0 | 16 | 101 | 117 | 202 | 4.68e-33 | 114 |
| MS.gene067526.t1 | AT1G18720 | 60.465 | 86 | 34 | 0 | 16 | 101 | 117 | 202 | 4.68e-33 | 114 |
| MS.gene067526.t1 | AT1G74440 | 58.025 | 81 | 34 | 0 | 16 | 96 | 117 | 197 | 8.20e-29 | 103 |
| MS.gene067526.t1 | AT1G74440 | 58.025 | 81 | 34 | 0 | 16 | 96 | 117 | 197 | 8.20e-29 | 103 |
Find 31 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCAAATATCAAAGAATTAA+AGG | 0.218517 | 3.2:-26523718 | MS.gene067526:CDS |
| TTTGTCTGAAATCCAGGATA+TGG | 0.368016 | 3.2:+26523759 | None:intergenic |
| GTGTTGGCTGCTCAGTTGAT+TGG | 0.382102 | 3.2:-26524075 | MS.gene067526:CDS |
| TTCACCTTTGTCTGAAATCC+AGG | 0.431535 | 3.2:+26523753 | None:intergenic |
| TATAATCTTTCAGGTTGTGT+TGG | 0.431542 | 3.2:-26524091 | MS.gene067526:intron |
| GGATGGACTGGAACATCTAT+TGG | 0.439686 | 3.2:-26524054 | MS.gene067526:CDS |
| GTTCATATCCAATGGATGAT+TGG | 0.446343 | 3.2:+26523781 | None:intergenic |
| ATTGGATATGAACCATATCC+TGG | 0.460320 | 3.2:-26523771 | MS.gene067526:CDS |
| TATTGGTCATGGCGTGTTTG+AGG | 0.463494 | 3.2:-26524037 | MS.gene067526:intron |
| TGGCTGCTCAGTTGATTGGA+TGG | 0.484630 | 3.2:-26524071 | MS.gene067526:CDS |
| ACCTCAAGAATCACGAAGAA+AGG | 0.495722 | 3.2:+26523885 | None:intergenic |
| GCTCAGTTGATTGGATGGAC+TGG | 0.499989 | 3.2:-26524066 | MS.gene067526:CDS |
| CGATTGGATGACCTTGCTCA+AGG | 0.509060 | 3.2:-26523921 | MS.gene067526:CDS |
| GAAACGTGTGTCAACTCGAT+TGG | 0.511678 | 3.2:-26523937 | MS.gene067526:CDS |
| GCCTTTCTTCGTGATTCTTG+AGG | 0.516103 | 3.2:-26523886 | MS.gene067526:intron |
| TAGGTCCTCCAATCATCCAT+TGG | 0.519762 | 3.2:-26523789 | MS.gene067526:CDS |
| AGGATATGGTTCATATCCAA+TGG | 0.559473 | 3.2:+26523773 | None:intergenic |
| GAATTGGCCACATACTTGGC+AGG | 0.562006 | 3.2:+26524408 | None:intergenic |
| CCGAGAGTAAATCTCGGAAG+AGG | 0.578420 | 3.2:+26524448 | None:intergenic |
| CATATCCAATGGATGATTGG+AGG | 0.588211 | 3.2:+26523784 | None:intergenic |
| TTCAGACAAAGGTGAAGGCA+AGG | 0.592728 | 3.2:-26523746 | MS.gene067526:CDS |
| TTGGCCACATACTTGGCAGG+TGG | 0.599646 | 3.2:+26524411 | None:intergenic |
| TGGCCACATACTTGGCAGGT+GGG | 0.609520 | 3.2:+26524412 | None:intergenic |
| TGCATACCGAGAGTAAATCT+CGG | 0.611737 | 3.2:+26524442 | None:intergenic |
| GCTCCTTTAGGAGTCATGAC+AGG | 0.616847 | 3.2:-26524475 | None:intergenic |
| ACTGGAACATCTATTGGTCA+TGG | 0.617255 | 3.2:-26524048 | MS.gene067526:CDS |
| CCTCTTCCGAGATTTACTCT+CGG | 0.620299 | 3.2:-26524448 | MS.gene067526:CDS |
| TGGATTTCAGACAAAGGTGA+AGG | 0.621966 | 3.2:-26523751 | MS.gene067526:CDS |
| CGAGAGTAAATCTCGGAAGA+GGG | 0.622972 | 3.2:+26524449 | None:intergenic |
| CCTTTAGGAGTCATGACAGG+AGG | 0.670470 | 3.2:-26524472 | None:intergenic |
| ATATCCTGGATTTCAGACAA+AGG | 0.705635 | 3.2:-26523757 | MS.gene067526:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACAATTGTGCTTTAATTA+TGG | - | chr3.2:26524036-26524055 | MS.gene067526:intron | 20.0% |
| !! | AGCAAATATCAAAGAATTAA+AGG | - | chr3.2:26524434-26524453 | MS.gene067526:CDS | 20.0% |
| !! | TTTCTTTCAAAATGAAAACA+AGG | - | chr3.2:26524318-26524337 | MS.gene067526:intron | 20.0% |
| !!! | ACTATCTTAATTTGTTTTGT+AGG | - | chr3.2:26524344-26524363 | MS.gene067526:intron | 20.0% |
| ! | ACAAAATATGTTTATGACGA+AGG | - | chr3.2:26523985-26524004 | MS.gene067526:intron | 25.0% |
| !! | ATTATGGACTATAATCTTTC+AGG | - | chr3.2:26524052-26524071 | MS.gene067526:CDS | 25.0% |
| !! | TCGTTTTACTCACTTATATA+TGG | + | chr3.2:26523879-26523898 | None:intergenic | 25.0% |
| ATGTTAGGCTACATTCATTA+TGG | - | chr3.2:26523813-26523832 | MS.gene067526:intron | 30.0% | |
| TACTCACTTATATATGGATG+TGG | + | chr3.2:26523873-26523892 | None:intergenic | 30.0% | |
| TTAAATTGAGACGGATGAAT+TGG | + | chr3.2:26523763-26523782 | None:intergenic | 30.0% | |
| TTTGTCATAGTGATTTGAAC+TGG | - | chr3.2:26524295-26524314 | MS.gene067526:intron | 30.0% | |
| !! | TATAATCTTTCAGGTTGTGT+TGG | - | chr3.2:26524061-26524080 | MS.gene067526:CDS | 30.0% |
| AGGATATGGTTCATATCCAA+TGG | + | chr3.2:26524382-26524401 | None:intergenic | 35.0% | |
| ATATCCTGGATTTCAGACAA+AGG | - | chr3.2:26524395-26524414 | MS.gene067526:intron | 35.0% | |
| ATTGGATATGAACCATATCC+TGG | - | chr3.2:26524381-26524400 | MS.gene067526:intron | 35.0% | |
| CCATTAAAAAACCTTGAGCA+AGG | + | chr3.2:26524245-26524264 | None:intergenic | 35.0% | |
| CTACATTCATTATGGTAGAG+GGG | - | chr3.2:26523821-26523840 | MS.gene067526:intron | 35.0% | |
| GCTACATTCATTATGGTAGA+GGG | - | chr3.2:26523820-26523839 | MS.gene067526:intron | 35.0% | |
| GTTCATATCCAATGGATGAT+TGG | + | chr3.2:26524374-26524393 | None:intergenic | 35.0% | |
| TCATGTGACTATGTGATGTT+AGG | - | chr3.2:26523798-26523817 | MS.gene067526:intron | 35.0% | |
| TGTGACAGCTTAAATTGAGA+CGG | + | chr3.2:26523772-26523791 | None:intergenic | 35.0% | |
| ! | TTTGTCTGAAATCCAGGATA+TGG | + | chr3.2:26524396-26524415 | None:intergenic | 35.0% |
| !!! | CCTTGCTCAAGGTTTTTTAA+TGG | - | chr3.2:26524242-26524261 | MS.gene067526:intron | 35.0% |
| ACCTCAAGAATCACGAAGAA+AGG | + | chr3.2:26524270-26524289 | None:intergenic | 40.0% | |
| ACTGGAACATCTATTGGTCA+TGG | - | chr3.2:26524104-26524123 | MS.gene067526:intron | 40.0% | |
| CATATCCAATGGATGATTGG+AGG | + | chr3.2:26524371-26524390 | None:intergenic | 40.0% | |
| GGCTACATTCATTATGGTAG+AGG | - | chr3.2:26523819-26523838 | MS.gene067526:intron | 40.0% | |
| TGCATACCGAGAGTAAATCT+CGG | + | chr3.2:26523713-26523732 | None:intergenic | 40.0% | |
| TGGATTTCAGACAAAGGTGA+AGG | - | chr3.2:26524401-26524420 | MS.gene067526:intron | 40.0% | |
| ! | TTCACCTTTGTCTGAAATCC+AGG | + | chr3.2:26524402-26524421 | None:intergenic | 40.0% |
| !! | TGCTTTTCAGTGATGGAACA+AGG | + | chr3.2:26524146-26524165 | None:intergenic | 40.0% |
| CCTCTTCCGAGATTTACTCT+CGG | - | chr3.2:26523704-26523723 | MS.gene067526:CDS | 45.0% | |
| CGAGAGTAAATCTCGGAAGA+GGG | + | chr3.2:26523706-26523725 | None:intergenic | 45.0% | |
| GAAACGTGTGTCAACTCGAT+TGG | - | chr3.2:26524215-26524234 | MS.gene067526:intron | 45.0% | |
| GCCTTTCTTCGTGATTCTTG+AGG | - | chr3.2:26524266-26524285 | MS.gene067526:intron | 45.0% | |
| GGATGAATTGGCCACATACT+TGG | + | chr3.2:26523751-26523770 | None:intergenic | 45.0% | |
| TAGGTCCTCCAATCATCCAT+TGG | - | chr3.2:26524363-26524382 | MS.gene067526:intron | 45.0% | |
| TATTGGTCATGGCGTGTTTG+AGG | - | chr3.2:26524115-26524134 | MS.gene067526:intron | 45.0% | |
| TTCAGACAAAGGTGAAGGCA+AGG | - | chr3.2:26524406-26524425 | MS.gene067526:intron | 45.0% | |
| ! | TCATTATGGTAGAGGGGTAC+TGG | - | chr3.2:26523827-26523846 | MS.gene067526:intron | 45.0% |
| ! | TCCATCACTGAAAAGCACGA+CGG | - | chr3.2:26524149-26524168 | MS.gene067526:intron | 45.0% |
| !! | CATTATGGTAGAGGGGTACT+GGG | - | chr3.2:26523828-26523847 | MS.gene067526:intron | 45.0% |
| !! | GGATGGACTGGAACATCTAT+TGG | - | chr3.2:26524098-26524117 | MS.gene067526:intron | 45.0% |
| CCGAGAGTAAATCTCGGAAG+AGG | + | chr3.2:26523707-26523726 | None:intergenic | 50.0% | |
| CGATTGGATGACCTTGCTCA+AGG | - | chr3.2:26524231-26524250 | MS.gene067526:intron | 50.0% | |
| GAATTGGCCACATACTTGGC+AGG | + | chr3.2:26523747-26523766 | None:intergenic | 50.0% | |
| GCTCAGTTGATTGGATGGAC+TGG | - | chr3.2:26524086-26524105 | MS.gene067526:intron | 50.0% | |
| TGGCTGCTCAGTTGATTGGA+TGG | - | chr3.2:26524081-26524100 | MS.gene067526:CDS | 50.0% | |
| ! | TCCGTCGTGCTTTTCAGTGA+TGG | + | chr3.2:26524153-26524172 | None:intergenic | 50.0% |
| !! | GTGTTGGCTGCTCAGTTGAT+TGG | - | chr3.2:26524077-26524096 | MS.gene067526:CDS | 50.0% |
| TGGCCACATACTTGGCAGGT+GGG | + | chr3.2:26523743-26523762 | None:intergenic | 55.0% | |
| TTGGCCACATACTTGGCAGG+TGG | + | chr3.2:26523744-26523763 | None:intergenic | 55.0% | |
| TCGCCCACCTGCCAAGTATG+TGG | - | chr3.2:26523737-26523756 | MS.gene067526:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 26523692 | 26524482 | 26523692 | ID=MS.gene067526 |
| chr3.2 | mRNA | 26523692 | 26524482 | 26523692 | ID=MS.gene067526.t1;Parent=MS.gene067526 |
| chr3.2 | exon | 26524423 | 26524482 | 26524423 | ID=MS.gene067526.t1.exon1;Parent=MS.gene067526.t1 |
| chr3.2 | CDS | 26524423 | 26524482 | 26524423 | ID=cds.MS.gene067526.t1;Parent=MS.gene067526.t1 |
| chr3.2 | exon | 26524038 | 26524100 | 26524038 | ID=MS.gene067526.t1.exon2;Parent=MS.gene067526.t1 |
| chr3.2 | CDS | 26524038 | 26524100 | 26524038 | ID=cds.MS.gene067526.t1;Parent=MS.gene067526.t1 |
| chr3.2 | exon | 26523887 | 26523958 | 26523887 | ID=MS.gene067526.t1.exon3;Parent=MS.gene067526.t1 |
| chr3.2 | CDS | 26523887 | 26523958 | 26523887 | ID=cds.MS.gene067526.t1;Parent=MS.gene067526.t1 |
| chr3.2 | exon | 26523692 | 26523808 | 26523692 | ID=MS.gene067526.t1.exon4;Parent=MS.gene067526.t1 |
| chr3.2 | CDS | 26523692 | 26523808 | 26523692 | ID=cds.MS.gene067526.t1;Parent=MS.gene067526.t1 |
| Gene Sequence |
| Protein sequence |
