AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene067581

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067581.t1 MTR_4g083660 95.174 373 18 0 26 398 2 374 0.0 734
MS.gene067581.t1 MTR_8g009810 63.476 397 143 2 3 398 5 400 0.0 525
MS.gene067581.t1 MTR_2g029170 67.857 336 104 3 65 398 28 361 5.86e-166 469
MS.gene067581.t1 MTR_2g049400 52.806 392 180 5 8 398 4 391 3.67e-138 400
MS.gene067581.t1 MTR_1g088160 41.207 381 210 9 28 398 30 406 6.58e-91 280
MS.gene067581.t1 MTR_1g052315 43.122 378 207 7 25 397 56 430 9.32e-91 281
MS.gene067581.t1 MTR_3g438050 38.945 398 226 10 11 398 5 395 8.51e-88 271
MS.gene067581.t1 MTR_7g091850 43.207 368 201 7 34 395 77 442 1.18e-85 268
MS.gene067581.t1 MTR_8g030930 38.596 399 230 9 10 398 5 398 1.14e-84 263
MS.gene067581.t1 MTR_2g103410 41.129 372 210 8 34 398 60 429 6.48e-84 263
MS.gene067581.t1 MTR_8g065030 40.318 377 205 11 34 398 66 434 1.08e-82 260
MS.gene067581.t1 MTR_2g058670 38.046 389 218 7 8 383 1 379 1.39e-82 259
MS.gene067581.t1 MTR_4g120320 37.634 372 221 7 34 397 53 421 2.88e-82 258
MS.gene067581.t1 MTR_6g028080 36.220 381 227 8 27 398 24 397 6.92e-82 256
MS.gene067581.t1 MTR_2g058840 37.726 387 220 7 8 383 1 377 8.52e-82 257
MS.gene067581.t1 MTR_2g435640 37.366 372 222 7 34 397 54 422 4.90e-81 255
MS.gene067581.t1 MTR_6g005630 40.318 377 212 10 27 398 39 407 2.80e-80 253
MS.gene067581.t1 MTR_2g058640 38.243 387 220 8 8 383 1 379 1.48e-79 251
MS.gene067581.t1 MTR_5g043350 36.476 403 241 9 4 398 1 396 7.31e-77 243
MS.gene067581.t1 MTR_8g010070 36.476 403 233 10 4 398 1 388 1.66e-75 239
MS.gene067581.t1 MTR_2g060350 40.407 344 192 9 35 370 76 414 9.01e-74 237
MS.gene067581.t1 MTR_3g030540 41.304 368 195 10 23 374 50 412 3.04e-73 235
MS.gene067581.t1 MTR_3g084380 37.330 367 214 7 13 371 1 359 2.16e-72 232
MS.gene067581.t1 MTR_4g084050 35.236 403 229 13 11 398 9 394 6.70e-71 228
MS.gene067581.t1 MTR_3g084360 35.156 384 234 10 12 387 3 379 7.48e-70 225
MS.gene067581.t1 MTR_7g091840 43.590 312 167 8 33 337 65 374 1.33e-69 224
MS.gene067581.t1 MTR_8g065010 34.815 405 226 9 29 398 103 504 3.69e-69 227
MS.gene067581.t1 MTR_5g034090 36.927 371 201 7 30 370 118 485 4.95e-69 227
MS.gene067581.t1 MTR_1g086390 36.504 389 226 12 28 398 76 461 1.34e-68 224
MS.gene067581.t1 MTR_2g103650 36.816 402 234 10 10 398 8 402 1.01e-67 220
MS.gene067581.t1 MTR_4g094715 37.629 388 221 14 28 398 60 443 1.09e-67 221
MS.gene067581.t1 MTR_7g110050 36.951 387 224 12 29 398 63 446 1.43e-67 221
MS.gene067581.t1 MTR_3g054320 34.988 403 243 8 4 398 1 392 1.61e-67 219
MS.gene067581.t1 MTR_8g008765 36.313 358 209 9 4 356 1 344 1.95e-67 217
MS.gene067581.t1 MTR_5g097860 37.963 324 189 8 28 344 20 338 1.23e-66 215
MS.gene067581.t1 MTR_8g010090 34.772 394 236 10 11 398 9 387 1.24e-64 211
MS.gene067581.t1 MTR_6g005630 37.135 377 201 12 27 398 39 384 2.48e-64 211
MS.gene067581.t1 MTR_8g010080 35.014 357 214 7 4 356 1 343 6.64e-64 209
MS.gene067581.t1 MTR_5g033680 34.211 380 229 12 32 396 58 431 7.15e-64 212
MS.gene067581.t1 MTR_8g010110 35.190 395 233 11 11 398 9 387 1.20e-63 209
MS.gene067581.t1 MTR_1g013740 33.500 400 235 12 7 398 12 388 1.68e-63 208
MS.gene067581.t1 MTR_7g113910 38.387 310 154 8 95 398 75 353 1.18e-62 206
MS.gene067581.t1 MTR_1g012390 34.949 392 237 11 11 394 9 390 1.90e-62 206
MS.gene067581.t1 MTR_0157s0060 37.197 371 214 9 36 398 1 360 3.38e-62 204
MS.gene067581.t1 MTR_4g034020 37.197 371 214 9 36 398 1 360 3.38e-62 204
MS.gene067581.t1 MTR_5g063430 38.170 317 182 7 11 323 6 312 8.66e-62 202
MS.gene067581.t1 MTR_1g013730 34.085 399 233 12 4 395 1 376 1.04e-61 204
MS.gene067581.t1 MTR_7g035415 35.826 321 186 8 7 316 9 320 2.48e-60 199
MS.gene067581.t1 MTR_8g010040 34.000 400 240 11 4 395 1 384 9.18e-60 199
MS.gene067581.t1 MTR_8g010050 33.501 397 207 9 8 398 9 354 1.21e-59 197
MS.gene067581.t1 MTR_2g032710 34.684 395 239 11 20 398 54 445 1.10e-57 195
MS.gene067581.t1 MTR_2g103410 42.248 258 142 6 34 285 60 316 2.34e-54 183
MS.gene067581.t1 MTR_5g034090 38.112 286 147 4 30 285 118 403 1.22e-53 183
MS.gene067581.t1 MTR_8g077320 33.046 348 170 10 26 358 26 325 2.01e-47 166
MS.gene067581.t1 MTR_8g008700 34.951 309 166 9 93 398 14 290 1.72e-46 161
MS.gene067581.t1 MTR_2g100040 31.044 364 191 10 10 368 8 316 2.13e-45 160
MS.gene067581.t1 MTR_8g031180 38.947 190 110 2 172 356 73 261 1.37e-33 127
MS.gene067581.t1 MTR_8g007320 58.904 73 30 0 326 398 22 94 1.18e-24 97.1
MS.gene067581.t1 MTR_4g124980 27.764 407 238 18 32 397 73 464 3.33e-23 101
MS.gene067581.t1 MTR_3g449590 27.415 383 223 15 27 370 41 407 1.77e-19 90.1
MS.gene067581.t1 MTR_7g110710 26.034 411 232 15 27 382 37 430 2.17e-19 90.1
MS.gene067581.t1 MTR_7g110710 26.034 411 232 15 27 382 37 430 2.35e-19 90.1
MS.gene067581.t1 MTR_7g088550 28.278 389 232 17 18 370 35 412 2.48e-19 90.1
MS.gene067581.t1 MTR_2g042740 25.296 423 264 16 14 397 37 446 5.34e-19 89.0
MS.gene067581.t1 MTR_8g098840 27.654 405 236 18 34 397 45 433 6.80e-19 88.6
MS.gene067581.t1 MTR_8g028725 25.537 419 265 14 14 397 38 444 1.94e-18 87.4
MS.gene067581.t1 MTR_5g090160 25.799 407 257 14 27 397 38 435 2.05e-18 87.0
MS.gene067581.t1 MTR_1g100050 26.478 423 253 19 18 397 32 439 2.24e-18 87.0
MS.gene067581.t1 MTR_1g100060 26.683 401 239 17 37 397 97 482 2.92e-18 87.0
MS.gene067581.t1 MTR_7g078680 25.776 419 264 15 14 397 38 444 1.70e-17 84.3
MS.gene067581.t1 MTR_1g099850 26.376 436 266 20 7 397 19 444 2.06e-17 84.3
MS.gene067581.t1 MTR_1g086030 29.316 307 173 12 35 307 84 380 2.50e-17 84.0
MS.gene067581.t1 MTR_1g100040 25.622 402 246 17 35 397 58 445 1.27e-16 81.6
MS.gene067581.t1 MTR_1g086030 34.197 193 107 7 128 307 91 276 2.00e-15 77.8
MS.gene067581.t1 MTR_4g120730 23.958 384 242 16 25 371 32 402 6.16e-15 76.6
MS.gene067581.t1 MTR_3g096330 25.466 322 175 12 34 310 77 378 9.09e-15 76.6
MS.gene067581.t1 MTR_7g072360 36.111 108 47 1 177 284 43 128 5.26e-12 63.2
MS.gene067581.t1 MTR_2g435340 24.586 362 214 12 35 368 39 369 3.34e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067581.t1 AT3G59850 66.216 370 123 2 30 398 64 432 0.0 517
MS.gene067581.t1 AT3G59850 66.216 370 123 2 30 398 20 388 0.0 515
MS.gene067581.t1 AT2G43870 62.663 383 139 4 17 398 5 384 1.31e-179 505
MS.gene067581.t1 AT1G05660 52.713 387 178 4 13 398 10 392 1.51e-148 426
MS.gene067581.t1 AT1G05650 52.455 387 179 4 13 398 10 392 2.42e-147 423
MS.gene067581.t1 AT2G43890 50.254 394 191 3 5 398 4 392 2.51e-143 413
MS.gene067581.t1 AT2G43860 55.409 379 167 2 21 398 21 398 4.07e-141 408
MS.gene067581.t1 AT1G65570 49.367 395 196 4 7 398 4 397 6.23e-141 407
MS.gene067581.t1 AT2G43880 50.541 370 181 2 29 398 27 394 2.68e-133 388
MS.gene067581.t1 AT2G43880 52.013 298 141 2 29 326 27 322 2.42e-106 317
MS.gene067581.t1 AT1G17150 41.960 398 214 8 8 398 14 401 4.21e-93 285
MS.gene067581.t1 AT3G07970 41.379 377 213 6 24 394 62 436 3.53e-92 284
MS.gene067581.t1 AT2G40310 39.851 404 226 11 8 398 4 403 3.82e-90 278
MS.gene067581.t1 AT4G13760 41.489 376 207 9 33 398 31 403 2.11e-89 276
MS.gene067581.t1 AT1G43080 40.769 390 213 10 24 398 17 403 2.42e-89 276
MS.gene067581.t1 AT2G15450 40.704 398 212 12 19 398 12 403 2.13e-87 271
MS.gene067581.t1 AT3G57510 41.935 372 203 7 30 395 65 429 4.49e-87 271
MS.gene067581.t1 AT1G02790 38.000 400 225 8 1 386 8 398 7.76e-87 270
MS.gene067581.t1 AT2G15470 40.201 398 214 12 19 398 12 403 8.57e-87 269
MS.gene067581.t1 AT1G70500 39.637 386 222 9 21 398 54 436 3.18e-86 270
MS.gene067581.t1 AT2G15460 40.506 395 211 12 19 395 12 400 5.33e-86 267
MS.gene067581.t1 AT1G43090 39.440 393 218 10 8 390 13 395 1.02e-85 268
MS.gene067581.t1 AT1G43100 40.576 382 209 10 24 390 17 395 1.07e-85 268
MS.gene067581.t1 AT2G33160 39.500 400 224 10 14 397 5 402 1.16e-85 274
MS.gene067581.t1 AT1G78400 40.054 372 216 6 32 398 34 403 3.46e-85 265
MS.gene067581.t1 AT4G18180 37.958 382 219 6 34 397 40 421 2.90e-84 263
MS.gene067581.t1 AT2G26620 40.909 374 210 8 33 398 31 401 8.90e-84 261
MS.gene067581.t1 AT1G80170 39.005 382 223 8 25 398 45 424 8.02e-83 260
MS.gene067581.t1 AT2G41850 41.176 374 201 9 31 395 66 429 1.61e-81 256
MS.gene067581.t1 AT3G07830 37.405 393 231 10 10 397 5 387 1.95e-81 255
MS.gene067581.t1 AT1G23460 40.933 386 213 12 23 398 32 412 7.01e-81 255
MS.gene067581.t1 AT1G23460 40.933 386 213 12 23 398 56 436 1.14e-80 255
MS.gene067581.t1 AT3G07840 38.133 375 218 9 30 397 20 387 1.58e-80 253
MS.gene067581.t1 AT3G07820 38.936 357 210 7 46 397 33 386 1.07e-79 251
MS.gene067581.t1 AT3G14040 39.314 379 218 6 26 397 64 437 1.45e-79 252
MS.gene067581.t1 AT5G48140 37.270 381 224 9 24 397 14 386 1.96e-78 247
MS.gene067581.t1 AT3G15720 38.760 387 218 7 20 398 13 388 5.60e-77 245
MS.gene067581.t1 AT3G15720 38.760 387 218 7 20 398 14 389 5.94e-77 245
MS.gene067581.t1 AT3G07850 38.503 374 218 6 31 397 68 436 2.89e-76 243
MS.gene067581.t1 AT3G15720 38.760 387 215 8 20 398 13 385 1.90e-75 241
MS.gene067581.t1 AT5G14650 37.795 381 214 9 8 370 11 386 1.95e-75 241
MS.gene067581.t1 AT4G35670 37.903 372 218 9 33 398 24 388 2.64e-74 237
MS.gene067581.t1 AT1G80140 41.691 343 182 9 66 398 2 336 5.47e-74 234
MS.gene067581.t1 AT1G56710 38.425 419 227 13 7 398 13 427 9.83e-73 234
MS.gene067581.t1 AT1G60590 37.010 408 213 11 30 398 120 522 2.82e-72 236
MS.gene067581.t1 AT5G27530 33.911 404 245 8 2 398 21 409 1.84e-71 231
MS.gene067581.t1 AT1G10640 35.980 403 221 10 30 398 116 515 2.77e-71 233
MS.gene067581.t1 AT5G17200 37.337 383 205 8 35 391 41 414 4.55e-71 229
MS.gene067581.t1 AT5G44840 34.656 378 223 10 33 398 24 389 1.73e-69 224
MS.gene067581.t1 AT1G02460 38.501 387 214 14 33 398 89 472 6.13e-69 226
MS.gene067581.t1 AT3G15720 39.275 331 186 5 75 398 3 325 2.49e-66 216
MS.gene067581.t1 AT4G01890 37.535 357 200 12 33 370 65 417 8.65e-65 214
MS.gene067581.t1 AT3G26610 35.171 381 225 10 33 396 67 442 1.16e-64 214
MS.gene067581.t1 AT1G48100 35.602 382 227 11 33 398 80 458 1.01e-63 211
MS.gene067581.t1 AT4G32380 37.349 332 193 7 47 370 41 365 1.50e-61 205
MS.gene067581.t1 AT5G44830 34.451 328 195 6 75 398 19 330 2.26e-58 193
MS.gene067581.t1 AT5G39910 32.530 415 203 11 6 398 9 368 3.16e-58 194
MS.gene067581.t1 AT4G32375 41.538 260 144 5 114 371 13 266 2.12e-57 194
MS.gene067581.t1 AT4G35670 37.979 287 169 6 114 398 2 281 3.22e-56 186
MS.gene067581.t1 AT4G32370 39.799 299 158 10 47 332 49 338 2.54e-55 186
MS.gene067581.t1 AT1G80170 40.075 267 153 6 25 285 45 310 4.00e-55 185
MS.gene067581.t1 AT5G14650 37.500 296 165 6 8 285 11 304 4.91e-55 184
MS.gene067581.t1 AT4G32380 32.827 329 171 5 47 370 41 324 3.05e-47 166
MS.gene067581.t1 AT3G48950 27.419 434 256 17 10 397 15 435 1.10e-23 102
MS.gene067581.t1 AT1G19170 28.013 307 193 11 110 397 181 478 9.21e-23 100
MS.gene067581.t1 AT4G23820 27.970 404 234 17 34 397 42 428 3.71e-21 95.1
MS.gene067581.t1 AT3G61490 25.450 444 271 16 4 397 1 434 5.99e-21 94.7
MS.gene067581.t1 AT3G61490 25.450 444 271 16 4 397 1 434 5.99e-21 94.7
MS.gene067581.t1 AT3G61490 25.450 444 271 16 4 397 1 434 5.99e-21 94.7
MS.gene067581.t1 AT3G62110 27.147 361 220 13 16 342 26 377 7.81e-21 94.4
MS.gene067581.t1 AT3G62110 27.147 361 220 13 16 342 26 377 7.81e-21 94.4
MS.gene067581.t1 AT3G61490 25.450 444 271 16 4 397 16 449 7.95e-21 94.7
MS.gene067581.t1 AT3G61490 25.450 444 271 16 4 397 114 547 1.73e-20 94.0
MS.gene067581.t1 AT3G42950 26.139 417 242 15 27 397 60 456 3.88e-20 92.4
MS.gene067581.t1 AT2G23900 26.434 401 241 15 37 397 60 446 1.54e-19 90.5
MS.gene067581.t1 AT5G49215 26.055 403 246 15 35 398 45 434 5.38e-19 89.0
MS.gene067581.t1 AT5G49215 26.055 403 246 15 35 398 43 432 5.90e-19 88.6
MS.gene067581.t1 AT5G41870 26.303 403 256 12 27 397 39 432 8.02e-19 88.2
MS.gene067581.t1 AT3G16850 25.116 430 270 13 7 397 10 426 1.71e-18 87.4
MS.gene067581.t1 AT4G33440 27.986 293 154 13 128 381 185 459 3.45e-16 80.5
MS.gene067581.t1 AT4G23500 24.638 414 259 19 22 397 59 457 5.68e-16 80.1
MS.gene067581.t1 AT3G06770 25.432 405 244 18 34 397 44 431 1.05e-12 69.7
MS.gene067581.t1 AT3G06770 25.432 405 244 18 34 397 45 432 1.14e-12 69.7

Find 109 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCATCACCGGTTCGAATTT+TGG 0.125064 4.1:-24332506 None:intergenic
CCGGGGCAGCCTTCGTTGTT+TGG 0.192728 4.1:-24332939 None:intergenic
TTGTTACTTAAATTCCTTTA+CGG 0.225568 4.1:+24331279 MS.gene067581:CDS
CGTTGTTTGGGCAATAATTC+TGG 0.240499 4.1:-24332926 None:intergenic
GTCGGTGTTTGGGCTGTTTC+CGG 0.278257 4.1:-24332440 None:intergenic
CTTGGAACTGCAACATTTAG+TGG 0.283715 4.1:+24331454 MS.gene067581:CDS
GGTCAATTACAATTGGGTTT+TGG 0.298931 4.1:-24332905 None:intergenic
CGGGGCAGCCTTCGTTGTTT+GGG 0.304377 4.1:-24332938 None:intergenic
ACGTGGATGCCGTCGGTGTT+TGG 0.336638 4.1:-24332451 None:intergenic
GCTATTTCCATAACCATTGA+TGG 0.348650 4.1:+24331493 MS.gene067581:CDS
TAATTCTGGTCAATTACAAT+TGG 0.351868 4.1:-24332912 None:intergenic
GTGGACCGGGACATGGAATA+AGG 0.352685 4.1:+24332581 MS.gene067581:CDS
ACATTCACAGGAACTCAAAA+TGG 0.357786 4.1:+24332801 MS.gene067581:CDS
ATCCGCGGCGGTGTGCTTGA+TGG 0.379802 4.1:+24331598 MS.gene067581:CDS
CAAAGTGCCATCAATGGTTA+TGG 0.385028 4.1:-24331500 None:intergenic
ATTTCTAAATGTATGGTCTA+AGG 0.389157 4.1:+24331378 MS.gene067581:CDS
CCACAAGGGAAATTCTTACT+TGG 0.390390 4.1:+24331436 MS.gene067581:CDS
CAGATGTTCCATGAATGTTT+TGG 0.392328 4.1:-24333188 None:intergenic
TGGTGACTTGGGAGGACATT+TGG 0.394523 4.1:-24332474 None:intergenic
CGTGGATGCCGTCGGTGTTT+GGG 0.397704 4.1:-24332450 None:intergenic
CCATGCTGGTGGAGCTGCAT+TGG 0.401798 4.1:+24333323 MS.gene067581:CDS
TACATTTAGAAATGCCTTTG+TGG 0.401933 4.1:-24331368 None:intergenic
TGGTGTTAGAATAAAGTCTT+GGG 0.413293 4.1:+24332821 MS.gene067581:CDS
GACAAAAGTAATTGCCCAAC+TGG 0.419215 4.1:+24331655 MS.gene067581:CDS
ACATACAATGTAATCAACTA+TGG 0.426913 4.1:+24331319 MS.gene067581:CDS
ACGTAAATTGCCGCCGGATA+AGG 0.427718 4.1:-24331412 None:intergenic
AATTCTGGTCAATTACAATT+GGG 0.433868 4.1:-24332911 None:intergenic
TCATCTAATTACCGTGTAAC+CGG 0.434207 4.1:+24331529 MS.gene067581:CDS
GGAGTTGAGGATGGTGACTT+GGG 0.435300 4.1:-24332485 None:intergenic
CAAAGGCATTTCTAAATGTA+TGG 0.435961 4.1:+24331371 MS.gene067581:CDS
GTTAAAACGGTTACATTCAC+AGG 0.436737 4.1:+24332789 MS.gene067581:CDS
CAACGAAGGCTGCCCCGGTC+AGG 0.438018 4.1:+24332944 MS.gene067581:CDS
GGTGATGATTGTATCTCAAT+TGG 0.439090 4.1:+24332520 MS.gene067581:CDS
GTAACATACCAAAACATTCA+TGG 0.441891 4.1:+24333180 MS.gene067581:CDS
CATTAACCATGATTGCATCT+TGG 0.446209 4.1:-24332881 None:intergenic
TACATGATGCTTCAGCCACT+TGG 0.449945 4.1:-24333299 None:intergenic
TGTTGCAGTATTGGAAGCTT+GGG 0.450951 4.1:+24332729 MS.gene067581:intron
TTGTTGCAGTATTGGAAGCT+TGG 0.451276 4.1:+24332728 MS.gene067581:intron
TGGAGTTGAGGATGGTGACT+TGG 0.452456 4.1:-24332486 None:intergenic
ATGGTGTTAGAATAAAGTCT+TGG 0.456513 4.1:+24332820 MS.gene067581:CDS
AAATTATTAAATCAGGCCTC+TGG 0.458353 4.1:+24333141 MS.gene067581:intron
CATAACCATTGATGGCACTT+TGG 0.461412 4.1:+24331501 MS.gene067581:CDS
TGAAGTTTCAGCATGTTGAC+GGG 0.462893 4.1:+24331569 MS.gene067581:CDS
CATGCTGGTGGAGCTGCATT+GGG 0.465376 4.1:+24333324 MS.gene067581:CDS
GCCCTGGTACAAGTAATTTG+TGG 0.471238 4.1:+24332542 MS.gene067581:CDS
CCAAACAACGAAGGCTGCCC+CGG 0.472065 4.1:+24332939 MS.gene067581:CDS
TTGAAGTTTCAGCATGTTGA+CGG 0.472330 4.1:+24331568 MS.gene067581:CDS
GTGATTATGATTAAGAGAAA+AGG 0.478653 4.1:-24331250 None:intergenic
GGCATGTTCCTCGCCTTATC+CGG 0.479418 4.1:+24331399 MS.gene067581:CDS
TGAAAACATTGCATGTGGAC+CGG 0.479846 4.1:+24332567 MS.gene067581:CDS
ACTATTCATCGATGTCAAAC+CGG 0.483988 4.1:-24332350 None:intergenic
GGTGTCTATAAGCAACTCTC+TGG 0.484363 4.1:-24333357 None:intergenic
GACATTACCGTGGCTCCAGT+TGG 0.484371 4.1:-24331670 None:intergenic
AACCATTGATGGCACTTTGG+TGG 0.486786 4.1:+24331504 MS.gene067581:CDS
ACAAAATGTGACAGTTAAAA+CGG 0.489424 4.1:+24332776 MS.gene067581:CDS
TTCATCTTTATTCCACTACA+TGG 0.491908 4.1:-24333252 None:intergenic
CTCAACTCCAAAATTCGAAC+CGG 0.494726 4.1:+24332499 MS.gene067581:CDS
GGCATCCATTGAACGCTATG+TGG 0.499118 4.1:-24332381 None:intergenic
ATGTTCCACATAGCGTTCAA+TGG 0.499526 4.1:+24332376 MS.gene067581:CDS
TGGACAAGGTACTGCCTTGT+GGG 0.500715 4.1:+24331618 MS.gene067581:CDS
GAAAACATTGCATGTGGACC+GGG 0.509461 4.1:+24332568 MS.gene067581:CDS
CGACGCCGCTTTCGCCGTAA+AGG 0.516630 4.1:-24331293 None:intergenic
ATTGCATGTGGACCGGGACA+TGG 0.516777 4.1:+24332574 MS.gene067581:CDS
ATCCACAAATTACTTGTACC+AGG 0.519856 4.1:-24332544 None:intergenic
ATGAAACCAATTACCTGACC+GGG 0.524105 4.1:-24332957 None:intergenic
GACCTAGCAATGGATTTGTA+AGG 0.526984 4.1:+24332847 MS.gene067581:CDS
ACATTACCGTGGCTCCAGTT+GGG 0.535346 4.1:-24331669 None:intergenic
TATGAAACCAATTACCTGAC+CGG 0.547446 4.1:-24332958 None:intergenic
TTCCTTACAAATCCATTGCT+AGG 0.550177 4.1:-24332849 None:intergenic
AAATTCCTTTACGGCGAAAG+CGG 0.552531 4.1:+24331288 MS.gene067581:CDS
AGCCACCAAAGTGCCATCAA+TGG 0.553338 4.1:-24331506 None:intergenic
AAGAACCTTATTCCATGTCC+CGG 0.555681 4.1:-24332586 None:intergenic
ACCACTCACTTTAATTCCAG+AGG 0.562833 4.1:-24333157 None:intergenic
GGGGTAAATATCATAGCAGC+CGG 0.565921 4.1:+24332421 MS.gene067581:CDS
GGCGGTGTGCTTGATGGACA+AGG 0.570853 4.1:+24331604 MS.gene067581:CDS
GTTGACGGGGTTTCGATCCG+CGG 0.574746 4.1:+24331583 MS.gene067581:CDS
AACTATGGAGCCAAGCCAGA+TGG 0.575309 4.1:+24331334 MS.gene067581:CDS
ATGTAGTGGAATAAAGATGA+AGG 0.576564 4.1:+24333254 MS.gene067581:CDS
TAATTGCCCAACTGGAGCCA+CGG 0.594866 4.1:+24331663 MS.gene067581:CDS
GATTGTATCTCAATTGGCCC+TGG 0.595028 4.1:+24332526 MS.gene067581:CDS
ATGGACAAGGTACTGCCTTG+TGG 0.600344 4.1:+24331617 MS.gene067581:CDS
AGTTCGAAGTATCCATGTAG+TGG 0.607051 4.1:+24333240 MS.gene067581:CDS
TCCACAAATTACTTGTACCA+GGG 0.607306 4.1:-24332543 None:intergenic
GCCTCTGGAATTAAAGTGAG+TGG 0.609415 4.1:+24333156 MS.gene067581:CDS
GGATGGAAGTTAAATGAACC+AGG 0.616130 4.1:+24332750 MS.gene067581:CDS
TGGATTGAAAACATTGCATG+TGG 0.620040 4.1:+24332562 MS.gene067581:CDS
GGAAACAGCCCAAACACCGA+CGG 0.621233 4.1:+24332442 MS.gene067581:CDS
CTTGGGAGGACATTTGGACG+TGG 0.621514 4.1:-24332468 None:intergenic
CCAAGTAAGAATTTCCCTTG+TGG 0.626504 4.1:-24331436 None:intergenic
AATTGAGATACAATCATCAC+CGG 0.627312 4.1:-24332518 None:intergenic
GCAGTATTGGAAGCTTGGGA+TGG 0.632087 4.1:+24332733 MS.gene067581:intron
ATGTTCCTCGCCTTATCCGG+CGG 0.639177 4.1:+24331402 MS.gene067581:CDS
CCAATGCAGCTCCACCAGCA+TGG 0.645581 4.1:-24333323 None:intergenic
TCTTGGGGTAGACCTAGCAA+TGG 0.646022 4.1:+24332837 MS.gene067581:CDS
TGTGGTACGTAAATTGCCGC+CGG 0.646318 4.1:-24331418 None:intergenic
GCATCATGTAACCATGCTGG+TGG 0.657419 4.1:+24333312 MS.gene067581:CDS
CATTTGGACGTGGATGCCGT+CGG 0.662987 4.1:-24332458 None:intergenic
GACGGGGTTTCGATCCGCGG+CGG 0.663495 4.1:+24331586 MS.gene067581:CDS
GAAGTTTCAGCATGTTGACG+GGG 0.664703 4.1:+24331570 MS.gene067581:CDS
AATTGCCGCCGGATAAGGCG+AGG 0.666819 4.1:-24331407 None:intergenic
GTCCATCAAGCACACCGCCG+CGG 0.670643 4.1:-24331600 None:intergenic
AATTATTGCCCAAACAACGA+AGG 0.671027 4.1:+24332930 MS.gene067581:CDS
GTTGAGGATGGTGACTTGGG+AGG 0.672905 4.1:-24332482 None:intergenic
GAAGCATCATGTAACCATGC+TGG 0.675230 4.1:+24333309 MS.gene067581:CDS
GCGGCAATTTACGTACCACA+AGG 0.682523 4.1:+24331421 MS.gene067581:CDS
TGGTAAAACTGATTCCACAA+AGG 0.694530 4.1:+24331354 MS.gene067581:CDS
GGTGTTAGAATAAAGTCTTG+GGG 0.707177 4.1:+24332822 MS.gene067581:CDS
CGGCAATTTACGTACCACAA+GGG 0.730607 4.1:+24331422 MS.gene067581:CDS
TGAAACCAATTACCTGACCG+GGG 0.733003 4.1:-24332956 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAATATGTCAATAAT+TGG + chr4.1:24331938-24331957 MS.gene067581:intron 10.0%
!!! TTTTATAATGAGAATAATAA+TGG + chr4.1:24332693-24332712 MS.gene067581:intron 10.0%
!! AAATGTTATATACAAATAAG+TGG + chr4.1:24332061-24332080 MS.gene067581:intron 15.0%
!! AATTAAAGATTAATAAAACC+AGG - chr4.1:24331753-24331772 None:intergenic 15.0%
!! TAAGATAATACTTTCTTAAA+AGG - chr4.1:24332644-24332663 None:intergenic 15.0%
!! TCAAAAAATATTATGATTAC+CGG + chr4.1:24332331-24332350 MS.gene067581:CDS 15.0%
!!! TGGTTAATTTTTTTTTTCTA+TGG + chr4.1:24332280-24332299 MS.gene067581:intron 15.0%
!!! TTAATTTTTTTTTTCTATGG+TGG + chr4.1:24332283-24332302 MS.gene067581:intron 15.0%
!!! TTTATTTTATTGAAGAAAAG+AGG + chr4.1:24332248-24332267 MS.gene067581:intron 15.0%
!!! TTTGAGTTTAATTATTTAGT+GGG + chr4.1:24331811-24331830 MS.gene067581:intron 15.0%
!!! TTTTGAGTTTAATTATTTAG+TGG + chr4.1:24331810-24331829 MS.gene067581:intron 15.0%
!! AAATTATAATCACGTTCTAT+TGG + chr4.1:24332137-24332156 MS.gene067581:intron 20.0%
!! ATTGGTTATATTATTCTTTC+TGG + chr4.1:24331956-24331975 MS.gene067581:intron 20.0%
!!! AATCTTTAATTTGAACAAGT+TGG + chr4.1:24331762-24331781 MS.gene067581:intron 20.0%
!!! TATCTGATTCAAATTTTAAC+CGG + chr4.1:24333021-24333040 MS.gene067581:intron 20.0%
!!! TTGTTACTTAAATTCCTTTA+CGG + chr4.1:24331279-24331298 MS.gene067581:CDS 20.0%
! AATTCTGGTCAATTACAATT+GGG - chr4.1:24332914-24332933 None:intergenic 25.0%
! ACAAAATGTGACAGTTAAAA+CGG + chr4.1:24332776-24332795 MS.gene067581:CDS 25.0%
! ACATACAATGTAATCAACTA+TGG + chr4.1:24331319-24331338 MS.gene067581:CDS 25.0%
! ATAGAATGATATACTAGTGT+AGG + chr4.1:24332085-24332104 MS.gene067581:intron 25.0%
! ATTTCTAAATGTATGGTCTA+AGG + chr4.1:24331378-24331397 MS.gene067581:CDS 25.0%
! GTGATTATGATTAAGAGAAA+AGG - chr4.1:24331253-24331272 None:intergenic 25.0%
! GTGGTTGAAATTATTAAATC+AGG + chr4.1:24333134-24333153 MS.gene067581:intron 25.0%
! TAAAACCAGGCAAATAAATA+AGG - chr4.1:24331740-24331759 None:intergenic 25.0%
! TAATTCTGGTCAATTACAAT+TGG - chr4.1:24332915-24332934 None:intergenic 25.0%
! TCAAACAAAATTGATGTAAC+CGG - chr4.1:24333043-24333062 None:intergenic 25.0%
! TTGGTTTCATAACATCTTAT+TGG + chr4.1:24332970-24332989 MS.gene067581:intron 25.0%
! TTTAATTATTTAGTGGGAGT+AGG + chr4.1:24331817-24331836 MS.gene067581:intron 25.0%
!! TTGAATATTTGTTGCAGTAT+TGG + chr4.1:24332720-24332739 MS.gene067581:intron 25.0%
AAATTATTAAATCAGGCCTC+TGG + chr4.1:24333141-24333160 MS.gene067581:intron 30.0%
AATTGAGATACAATCATCAC+CGG - chr4.1:24332521-24332540 None:intergenic 30.0%
ATACTTAATATGCAACTGCT+TGG + chr4.1:24331864-24331883 MS.gene067581:intron 30.0%
CCAAAATGTAAAAACACAAG+GGG + chr4.1:24332402-24332421 MS.gene067581:CDS 30.0%
CTTGTGGGATTGTAAAAATT+CGG + chr4.1:24331633-24331652 MS.gene067581:CDS 30.0%
GCCAAAATGTAAAAACACAA+GGG + chr4.1:24332401-24332420 MS.gene067581:CDS 30.0%
GTAACATACCAAAACATTCA+TGG + chr4.1:24333180-24333199 MS.gene067581:CDS 30.0%
GTATATAGAAGTTGAAGCAT+GGG + chr4.1:24331897-24331916 MS.gene067581:intron 30.0%
TAACCGGTAAAAATGGTAAT+TGG + chr4.1:24331545-24331564 MS.gene067581:CDS 30.0%
TACATTTAGAAATGCCTTTG+TGG - chr4.1:24331371-24331390 None:intergenic 30.0%
TATGTCTGATCAATTCGAAT+TGG - chr4.1:24332022-24332041 None:intergenic 30.0%
TCACGTTCTATTGGAATTAT+TGG + chr4.1:24332146-24332165 MS.gene067581:intron 30.0%
TGCCAAAATGTAAAAACACA+AGG + chr4.1:24332400-24332419 MS.gene067581:CDS 30.0%
TTCATCTTTATTCCACTACA+TGG - chr4.1:24333255-24333274 None:intergenic 30.0%
! ACATCAACTTTGTTTGACTA+GGG - chr4.1:24333002-24333021 None:intergenic 30.0%
! ATGGTGTTAGAATAAAGTCT+TGG + chr4.1:24332820-24332839 MS.gene067581:CDS 30.0%
! ATGTAGTGGAATAAAGATGA+AGG + chr4.1:24333254-24333273 MS.gene067581:CDS 30.0%
! TACATCAACTTTGTTTGACT+AGG - chr4.1:24333003-24333022 None:intergenic 30.0%
! TAGGCAATGATATAATGGAA+CGG + chr4.1:24331836-24331855 MS.gene067581:intron 30.0%
! TGGTGTTAGAATAAAGTCTT+GGG + chr4.1:24332821-24332840 MS.gene067581:CDS 30.0%
!! ATTGGTTTGCGTAATATTGA+AGG - chr4.1:24332004-24332023 None:intergenic 30.0%
!! CAAAGGCATTTCTAAATGTA+TGG + chr4.1:24331371-24331390 MS.gene067581:CDS 30.0%
!! CAACCAATTACCATTTTTAC+CGG - chr4.1:24331551-24331570 None:intergenic 30.0%
!! GAGCTGATTATATGTTTTTG+TGG + chr4.1:24333115-24333134 MS.gene067581:intron 30.0%
AATTATTGCCCAAACAACGA+AGG + chr4.1:24332930-24332949 MS.gene067581:CDS 35.0%
ACATTCACAGGAACTCAAAA+TGG + chr4.1:24332801-24332820 MS.gene067581:CDS 35.0%
ACTATTCATCGATGTCAAAC+CGG - chr4.1:24332353-24332372 None:intergenic 35.0%
ATCCACAAATTACTTGTACC+AGG - chr4.1:24332547-24332566 None:intergenic 35.0%
CATTAACCATGATTGCATCT+TGG - chr4.1:24332884-24332903 None:intergenic 35.0%
GAGATAATTAGACATTACCG+TGG - chr4.1:24331683-24331702 None:intergenic 35.0%
GGTATATAGAAGTTGAAGCA+TGG + chr4.1:24331896-24331915 MS.gene067581:intron 35.0%
GGTGATGATTGTATCTCAAT+TGG + chr4.1:24332520-24332539 MS.gene067581:CDS 35.0%
GGTGTTAGAATAAAGTCTTG+GGG + chr4.1:24332822-24332841 MS.gene067581:CDS 35.0%
GTACAAAGCAAAAAGCAACT+TGG - chr4.1:24332199-24332218 None:intergenic 35.0%
GTTAAAACGGTTACATTCAC+AGG + chr4.1:24332789-24332808 MS.gene067581:CDS 35.0%
TATGAAACCAATTACCTGAC+CGG - chr4.1:24332961-24332980 None:intergenic 35.0%
TCAGACATAAGAATCAGAAG+AGG + chr4.1:24332033-24332052 MS.gene067581:intron 35.0%
TCATCTAATTACCGTGTAAC+CGG + chr4.1:24331529-24331548 MS.gene067581:CDS 35.0%
TCCACAAATTACTTGTACCA+GGG - chr4.1:24332546-24332565 None:intergenic 35.0%
TGGATTGAAAACATTGCATG+TGG + chr4.1:24332562-24332581 MS.gene067581:CDS 35.0%
TTCCTTACAAATCCATTGCT+AGG - chr4.1:24332852-24332871 None:intergenic 35.0%
TTGAAGTTTCAGCATGTTGA+CGG + chr4.1:24331568-24331587 MS.gene067581:CDS 35.0%
! CAGATGTTCCATGAATGTTT+TGG - chr4.1:24333191-24333210 None:intergenic 35.0%
! CGAATTTTTACAATCCCACA+AGG - chr4.1:24331635-24331654 None:intergenic 35.0%
! CTTTTTCCAAGATGCAATCA+TGG + chr4.1:24332875-24332894 MS.gene067581:CDS 35.0%
! GCTATTTCCATAACCATTGA+TGG + chr4.1:24331493-24331512 MS.gene067581:CDS 35.0%
! GGTCAATTACAATTGGGTTT+TGG - chr4.1:24332908-24332927 None:intergenic 35.0%
! TGGTAAAACTGATTCCACAA+AGG + chr4.1:24331354-24331373 MS.gene067581:CDS 35.0%
! TTGCACCTTATTTATTTGCC+TGG + chr4.1:24331732-24331751 MS.gene067581:intron 35.0%
!! CCCCTTGTGTTTTTACATTT+TGG - chr4.1:24332405-24332424 None:intergenic 35.0%
!! TACCATTTTTACCGGTTACA+CGG - chr4.1:24331543-24331562 None:intergenic 35.0%
AAATTCCTTTACGGCGAAAG+CGG + chr4.1:24331288-24331307 MS.gene067581:CDS 40.0%
AAGAACCTTATTCCATGTCC+CGG - chr4.1:24332589-24332608 None:intergenic 40.0%
ACCACTCACTTTAATTCCAG+AGG - chr4.1:24333160-24333179 None:intergenic 40.0%
ATGAAACCAATTACCTGACC+GGG - chr4.1:24332960-24332979 None:intergenic 40.0%
ATGTTCCACATAGCGTTCAA+TGG + chr4.1:24332376-24332395 MS.gene067581:CDS 40.0%
CCAAGTAAGAATTTCCCTTG+TGG - chr4.1:24331439-24331458 None:intergenic 40.0%
CCACAAGGGAAATTCTTACT+TGG + chr4.1:24331436-24331455 MS.gene067581:CDS 40.0%
CGTTGTTTGGGCAATAATTC+TGG - chr4.1:24332929-24332948 None:intergenic 40.0%
CTCAACTCCAAAATTCGAAC+CGG + chr4.1:24332499-24332518 MS.gene067581:CDS 40.0%
CTTGGAACTGCAACATTTAG+TGG + chr4.1:24331454-24331473 MS.gene067581:CDS 40.0%
GACAAAAGTAATTGCCCAAC+TGG + chr4.1:24331655-24331674 MS.gene067581:CDS 40.0%
GCTCACTTACAAAAACCAAG+TGG + chr4.1:24333284-24333303 MS.gene067581:CDS 40.0%
GGATGGAAGTTAAATGAACC+AGG + chr4.1:24332750-24332769 MS.gene067581:CDS 40.0%
GGGAGTAGGCAATGATATAA+TGG + chr4.1:24331831-24331850 MS.gene067581:intron 40.0%
TACCGTGTAACCGGTAAAAA+TGG + chr4.1:24331538-24331557 MS.gene067581:CDS 40.0%
TGAAAACATTGCATGTGGAC+CGG + chr4.1:24332567-24332586 MS.gene067581:CDS 40.0%
TGAAGTTTCAGCATGTTGAC+GGG + chr4.1:24331569-24331588 MS.gene067581:CDS 40.0%
! AACTTTGTTTGACTAGGGTG+AGG - chr4.1:24332997-24333016 None:intergenic 40.0%
! ACTGTCACATTTTGTACTCC+TGG - chr4.1:24332771-24332790 None:intergenic 40.0%
! AGTTCGAAGTATCCATGTAG+TGG + chr4.1:24333240-24333259 MS.gene067581:CDS 40.0%
! ATCAGTTTTACCATCTGGCT+TGG - chr4.1:24331347-24331366 None:intergenic 40.0%
! CAAAGTGCCATCAATGGTTA+TGG - chr4.1:24331503-24331522 None:intergenic 40.0%
! CATAACCATTGATGGCACTT+TGG + chr4.1:24331501-24331520 MS.gene067581:CDS 40.0%
! GAACAAGTTGGAGTTAACTC+AGG + chr4.1:24331774-24331793 MS.gene067581:intron 40.0%
! GACCTAGCAATGGATTTGTA+AGG + chr4.1:24332847-24332866 MS.gene067581:CDS 40.0%
! GTGGAATCAGTTTTACCATC+TGG - chr4.1:24331352-24331371 None:intergenic 40.0%
! TGTTGCAGTATTGGAAGCTT+GGG + chr4.1:24332729-24332748 MS.gene067581:intron 40.0%
! TTGTTGCAGTATTGGAAGCT+TGG + chr4.1:24332728-24332747 MS.gene067581:intron 40.0%
!! AAGAAAAGAGGTGTTGACAG+TGG + chr4.1:24332260-24332279 MS.gene067581:intron 40.0%
!!! ATCATCACCGGTTCGAATTT+TGG - chr4.1:24332509-24332528 None:intergenic 40.0%
!!! TCGAATTTTGGAGTTGAGGA+TGG - chr4.1:24332497-24332516 None:intergenic 40.0%
CGGCAATTTACGTACCACAA+GGG + chr4.1:24331422-24331441 MS.gene067581:CDS 45.0%
GAAAACATTGCATGTGGACC+GGG + chr4.1:24332568-24332587 MS.gene067581:CDS 45.0%
GAAGTTTCAGCATGTTGACG+GGG + chr4.1:24331570-24331589 MS.gene067581:CDS 45.0%
GATTGTATCTCAATTGGCCC+TGG + chr4.1:24332526-24332545 MS.gene067581:CDS 45.0%
GCCCTGGTACAAGTAATTTG+TGG + chr4.1:24332542-24332561 MS.gene067581:CDS 45.0%
GGGGTAAATATCATAGCAGC+CGG + chr4.1:24332421-24332440 MS.gene067581:CDS 45.0%
TACATGATGCTTCAGCCACT+TGG - chr4.1:24333302-24333321 None:intergenic 45.0%
TGAAACCAATTACCTGACCG+GGG - chr4.1:24332959-24332978 None:intergenic 45.0%
! AACCATTGATGGCACTTTGG+TGG + chr4.1:24331504-24331523 MS.gene067581:CDS 45.0%
! GAAGCATCATGTAACCATGC+TGG + chr4.1:24333309-24333328 MS.gene067581:CDS 45.0%
!! GCCTCTGGAATTAAAGTGAG+TGG + chr4.1:24333156-24333175 MS.gene067581:CDS 45.0%
!!! CGGTTCGAATTTTGGAGTTG+AGG - chr4.1:24332501-24332520 None:intergenic 45.0%
AACTATGGAGCCAAGCCAGA+TGG + chr4.1:24331334-24331353 MS.gene067581:CDS 50.0%
ACATTACCGTGGCTCCAGTT+GGG - chr4.1:24331672-24331691 None:intergenic 50.0%
ACGTAAATTGCCGCCGGATA+AGG - chr4.1:24331415-24331434 None:intergenic 50.0%
GCAACTGCTTGGTCTAGTTG+CGG + chr4.1:24331875-24331894 MS.gene067581:intron 50.0%
GCATCATGTAACCATGCTGG+TGG + chr4.1:24333312-24333331 MS.gene067581:CDS 50.0%
GCGGCAATTTACGTACCACA+AGG + chr4.1:24331421-24331440 MS.gene067581:CDS 50.0%
GGAGTTGAGGATGGTGACTT+GGG - chr4.1:24332488-24332507 None:intergenic 50.0%
GGCATCCATTGAACGCTATG+TGG - chr4.1:24332384-24332403 None:intergenic 50.0%
TAATTGCCCAACTGGAGCCA+CGG + chr4.1:24331663-24331682 MS.gene067581:CDS 50.0%
TCTTGGGGTAGACCTAGCAA+TGG + chr4.1:24332837-24332856 MS.gene067581:CDS 50.0%
TGGAGTTGAGGATGGTGACT+TGG - chr4.1:24332489-24332508 None:intergenic 50.0%
TGTGGTACGTAAATTGCCGC+CGG - chr4.1:24331421-24331440 None:intergenic 50.0%
! AGCCACCAAAGTGCCATCAA+TGG - chr4.1:24331509-24331528 None:intergenic 50.0%
! CTATGGTGGTTTTCAGACGC+TGG + chr4.1:24332297-24332316 MS.gene067581:intron 50.0%
! GCAGTATTGGAAGCTTGGGA+TGG + chr4.1:24332733-24332752 MS.gene067581:intron 50.0%
! TGGTGACTTGGGAGGACATT+TGG - chr4.1:24332477-24332496 None:intergenic 50.0%
!! ATGGACAAGGTACTGCCTTG+TGG + chr4.1:24331617-24331636 MS.gene067581:CDS 50.0%
!! TGGACAAGGTACTGCCTTGT+GGG + chr4.1:24331618-24331637 MS.gene067581:CDS 50.0%
ATGTTCCTCGCCTTATCCGG+CGG + chr4.1:24331402-24331421 MS.gene067581:CDS 55.0%
ATTGCATGTGGACCGGGACA+TGG + chr4.1:24332574-24332593 MS.gene067581:CDS 55.0%
CATGCTGGTGGAGCTGCATT+GGG + chr4.1:24333324-24333343 MS.gene067581:CDS 55.0%
CATTTGGACGTGGATGCCGT+CGG - chr4.1:24332461-24332480 None:intergenic 55.0%
CTTGGGAGGACATTTGGACG+TGG - chr4.1:24332471-24332490 None:intergenic 55.0%
GACATTACCGTGGCTCCAGT+TGG - chr4.1:24331673-24331692 None:intergenic 55.0%
GGAAACAGCCCAAACACCGA+CGG + chr4.1:24332442-24332461 MS.gene067581:CDS 55.0%
GGCATGTTCCTCGCCTTATC+CGG + chr4.1:24331399-24331418 MS.gene067581:CDS 55.0%
GTGGACCGGGACATGGAATA+AGG + chr4.1:24332581-24332600 MS.gene067581:CDS 55.0%
! GTCGGTGTTTGGGCTGTTTC+CGG - chr4.1:24332443-24332462 None:intergenic 55.0%
! GTTGAGGATGGTGACTTGGG+AGG - chr4.1:24332485-24332504 None:intergenic 55.0%
AATTGCCGCCGGATAAGGCG+AGG - chr4.1:24331410-24331429 None:intergenic 60.0%
CCAAACAACGAAGGCTGCCC+CGG + chr4.1:24332939-24332958 MS.gene067581:CDS 60.0%
CCAATGCAGCTCCACCAGCA+TGG - chr4.1:24333326-24333345 None:intergenic 60.0%
CCATGCTGGTGGAGCTGCAT+TGG + chr4.1:24333323-24333342 MS.gene067581:CDS 60.0%
CGGGGCAGCCTTCGTTGTTT+GGG - chr4.1:24332941-24332960 None:intergenic 60.0%
GTTGACGGGGTTTCGATCCG+CGG + chr4.1:24331583-24331602 MS.gene067581:CDS 60.0%
! ACGTGGATGCCGTCGGTGTT+TGG - chr4.1:24332454-24332473 None:intergenic 60.0%
! CGTGGATGCCGTCGGTGTTT+GGG - chr4.1:24332453-24332472 None:intergenic 60.0%
! GGCGGTGTGCTTGATGGACA+AGG + chr4.1:24331604-24331623 MS.gene067581:CDS 60.0%
ATCCGCGGCGGTGTGCTTGA+TGG + chr4.1:24331598-24331617 MS.gene067581:CDS 65.0%
CCGGGGCAGCCTTCGTTGTT+TGG - chr4.1:24332942-24332961 None:intergenic 65.0%
GGCTGCCCCGGTCAGGTAAT+TGG + chr4.1:24332951-24332970 MS.gene067581:intron 65.0%
GTCCATCAAGCACACCGCCG+CGG - chr4.1:24331603-24331622 None:intergenic 65.0%
! CGACGCCGCTTTCGCCGTAA+AGG - chr4.1:24331296-24331315 None:intergenic 65.0%
CAACGAAGGCTGCCCCGGTC+AGG + chr4.1:24332944-24332963 MS.gene067581:CDS 70.0%
GACGGGGTTTCGATCCGCGG+CGG + chr4.1:24331586-24331605 MS.gene067581:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 24331226 24333374 24331226 ID=MS.gene067581
chr4.1 mRNA 24331226 24333374 24331226 ID=MS.gene067581.t1;Parent=MS.gene067581
chr4.1 exon 24331226 24331684 24331226 ID=MS.gene067581.t1.exon1;Parent=MS.gene067581.t1
chr4.1 CDS 24331226 24331684 24331226 ID=cds.MS.gene067581.t1;Parent=MS.gene067581.t1
chr4.1 exon 24332313 24332602 24332313 ID=MS.gene067581.t1.exon2;Parent=MS.gene067581.t1
chr4.1 CDS 24332313 24332602 24332313 ID=cds.MS.gene067581.t1;Parent=MS.gene067581.t1
chr4.1 exon 24332737 24332965 24332737 ID=MS.gene067581.t1.exon3;Parent=MS.gene067581.t1
chr4.1 CDS 24332737 24332965 24332737 ID=cds.MS.gene067581.t1;Parent=MS.gene067581.t1
chr4.1 exon 24333156 24333374 24333156 ID=MS.gene067581.t1.exon4;Parent=MS.gene067581.t1
chr4.1 CDS 24333156 24333374 24333156 ID=cds.MS.gene067581.t1;Parent=MS.gene067581.t1
Gene Sequence

>MS.gene067581

ATGTCAAAACTTAACAATCTTCTTCCTTTTCTCTTAATCATAATCACATTATCTTGTTACTTAAATTCCTTTACGGCGAAAGCGGCGTCGTCGACATACAATGTAATCAACTATGGAGCCAAGCCAGATGGTAAAACTGATTCCACAAAGGCATTTCTAAATGTATGGTCTAAGGCATGTTCCTCGCCTTATCCGGCGGCAATTTACGTACCACAAGGGAAATTCTTACTTGGAACTGCAACATTTAGTGGAAAATGTATCAACAAAGCTATTTCCATAACCATTGATGGCACTTTGGTGGCTTCATCTAATTACCGTGTAACCGGTAAAAATGGTAATTGGTTGAAGTTTCAGCATGTTGACGGGGTTTCGATCCGCGGCGGTGTGCTTGATGGACAAGGTACTGCCTTGTGGGATTGTAAAAATTCGGACAAAAGTAATTGCCCAACTGGAGCCACGGTAATGTCTAATTATCTCATGCATTAGCATTGTATATGATTATTATTTTGCACCTTATTTATTTGCCTGGTTTTATTAATCTTTAATTTGAACAAGTTGGAGTTAACTCAGGTGATCAGTTACATTTTTGAGTTTAATTATTTAGTGGGAGTAGGCAATGATATAATGGAACGGTTAATATACTTAATATGCAACTGCTTGGTCTAGTTGCGGTATATAGAAGTTGAAGCATGGGAGTTAGTATAATATGCATAAAAATAATATGTCAATAATTGGTTATATTATTCTTTCTGGTTTGTTTATAAATCATATTGCACCTTCAATATTACGCAAACCAATTCGAATTGATCAGACATAAGAATCAGAAGAGGAAACAAAATGTTATATACAAATAAGTGGAATAGAATGATATACTAGTGTAGGATAATGCAATAGCTTGTAAAGTCATCAAGAAATTATAATCACGTTCTATTGGAATTATTGGTTTAAAACAAGTCAATTTTACTCACAACCAAGTTGCTTTTTGCTTTGTACAAAAGATAGTAATTATTTGATGATTTTATTTTATTTTATTGAAGAAAAGAGGTGTTGACAGTGGTTAATTTTTTTTTTCTATGGTGGTTTTCAGACGCTGGAATTCAGTAGCTCAAAAAATATTATGATTACCGGTTTGACATCGATGAATAGTCAAATGTTCCACATAGCGTTCAATGGATGCCAAAATGTAAAAACACAAGGGGTAAATATCATAGCAGCCGGAAACAGCCCAAACACCGACGGCATCCACGTCCAAATGTCCTCCCAAGTCACCATCCTCAACTCCAAAATTCGAACCGGTGATGATTGTATCTCAATTGGCCCTGGTACAAGTAATTTGTGGATTGAAAACATTGCATGTGGACCGGGACATGGAATAAGGTTCTTTTTTATTACAATTTTTGTTTCTTCTTAATTGTCCTTTTAAGAAAGTATTATCTTATTTAAAAATTGTTTTGTTTACTTTTATCATTTTATAATGAGAATAATAATGGTTTGTTGAATATTTGTTGCAGTATTGGAAGCTTGGGATGGAAGTTAAATGAACCAGGAGTACAAAATGTGACAGTTAAAACGGTTACATTCACAGGAACTCAAAATGGTGTTAGAATAAAGTCTTGGGGTAGACCTAGCAATGGATTTGTAAGGAACATCTTTTTCCAAGATGCAATCATGGTTAATGTCCAAAACCCAATTGTAATTGACCAGAATTATTGCCCAAACAACGAAGGCTGCCCCGGTCAGGTAATTGGTTTCATAACATCTTATTGGACCTCACCCTAGTCAAACAAAGTTGATGTATCTGATTCAAATTTTAACCGGTTACATCAATTTTGTTTGACTTTTTTTGCTTATATTAGATATAGAAATAATACAATTGTTAGCATGAAATTGAGCTGATTATATGTTTTTGTGGTTGAAATTATTAAATCAGGCCTCTGGAATTAAAGTGAGTGGTGTAACATACCAAAACATTCATGGAACATCTGCAACACAAGTAGCTGTGAAATTTGATTGTAGTTCGAAGTATCCATGTAGTGGAATAAAGATGAAGGATGTAAAGCTCACTTACAAAAACCAAGTGGCTGAAGCATCATGTAACCATGCTGGTGGAGCTGCATTGGGTTCAGTTCAGCCAGAGAGTTGCTTATAG
Protein sequence

>MS.gene067581.t1

MSKLNNLLPFLLIIITLSCYLNSFTAKAASSTYNVINYGAKPDGKTDSTKAFLNVWSKACSSPYPAAIYVPQGKFLLGTATFSGKCINKAISITIDGTLVASSNYRVTGKNGNWLKFQHVDGVSIRGGVLDGQGTALWDCKNSDKSNCPTGATTLEFSSSKNIMITGLTSMNSQMFHIAFNGCQNVKTQGVNIIAAGNSPNTDGIHVQMSSQVTILNSKIRTGDDCISIGPGTSNLWIENIACGPGHGISIGSLGWKLNEPGVQNVTVKTVTFTGTQNGVRIKSWGRPSNGFVRNIFFQDAIMVNVQNPIVIDQNYCPNNEGCPGQASGIKVSGVTYQNIHGTSATQVAVKFDCSSKYPCSGIKMKDVKLTYKNQVAEASCNHAGGAALGSVQPESCL