Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068044.t1 | XP_003608412.1 | 89.2 | 212 | 23 | 0 | 1 | 212 | 1 | 212 | 2.00E-87 | 332 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068044.t1 | B7FMC3 | 89.2 | 212 | 23 | 0 | 1 | 212 | 1 | 212 | 1.5e-87 | 332.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055144 | MS.gene068044 | 0.809598 | 1.75E-50 | -1.69E-46 |
| MS.gene058360 | MS.gene068044 | 0.802243 | 6.15E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068044.t1 | MTR_4g093820 | 91.509 | 212 | 18 | 0 | 1 | 212 | 1 | 212 | 8.36e-144 | 399 |
| MS.gene068044.t1 | MTR_4g093820 | 91.935 | 186 | 15 | 0 | 1 | 186 | 1 | 186 | 8.00e-126 | 353 |
| MS.gene068044.t1 | MTR_4g093820 | 90.972 | 144 | 13 | 0 | 69 | 212 | 15 | 158 | 9.75e-92 | 266 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068044.t1 | AT2G14095 | 43.299 | 194 | 102 | 5 | 28 | 214 | 27 | 219 | 3.34e-45 | 150 |
| MS.gene068044.t1 | AT2G14095 | 39.896 | 193 | 107 | 5 | 28 | 214 | 27 | 216 | 1.11e-35 | 125 |
Find 39 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCACAATCTTGTCAATTA+AGG | 0.115833 | 4.3:+20719662 | None:intergenic |
| GAACAACATGCAGGGTTAAA+TGG | 0.188381 | 4.3:-20720235 | MS.gene068044:intron |
| TACCATTCTGGAGTCAAAAT+TGG | 0.223283 | 4.3:-20720184 | MS.gene068044:CDS |
| TTTCTTTCGCACTACCATTC+TGG | 0.272847 | 4.3:-20720196 | MS.gene068044:CDS |
| AATTTAGAAGCTGGGGCTTC+AGG | 0.285272 | 4.3:+20720750 | None:intergenic |
| TTCACAATCTTGTCAATTAA+GGG | 0.322082 | 4.3:+20719663 | None:intergenic |
| ACCCTAGTTGTTGTCACAAT+TGG | 0.346240 | 4.3:+20720970 | None:intergenic |
| TGACTCCACCATAGCTTGAT+TGG | 0.352375 | 4.3:+20721004 | None:intergenic |
| ATTAGTGCTTGTGTTGCTTT+TGG | 0.366274 | 4.3:+20720900 | None:intergenic |
| ACCATTCTGGAGTCAAAATT+GGG | 0.374919 | 4.3:-20720183 | MS.gene068044:CDS |
| ACACAAGCACTAATGCAAAT+AGG | 0.384766 | 4.3:-20720891 | MS.gene068044:intron |
| GCTTCAAAACAAGCTGTTCA+TGG | 0.405746 | 4.3:-20719863 | MS.gene068044:CDS |
| TTGAAAGTAGCTATTGGAAT+AGG | 0.412756 | 4.3:-20720948 | MS.gene068044:CDS |
| CTAGGGTTGAAAGTAGCTAT+TGG | 0.418698 | 4.3:-20720954 | MS.gene068044:CDS |
| TTCTTTAGCTTAGCATCCTC+AGG | 0.420506 | 4.3:+20719908 | None:intergenic |
| AACAACATGCAGGGTTAAAT+GGG | 0.429811 | 4.3:-20720234 | MS.gene068044:intron |
| GGAAAACTTCAAAGAATAGA+AGG | 0.432806 | 4.3:-20720161 | MS.gene068044:intron |
| GAAGCAGAGATTGTGATTGA+AGG | 0.436716 | 4.3:-20720010 | MS.gene068044:CDS |
| AAGCAGAGATTGTGATTGAA+GGG | 0.466114 | 4.3:-20720009 | MS.gene068044:CDS |
| CTCACTTACCTTCTCTGCTA+AGG | 0.473583 | 4.3:+20719949 | None:intergenic |
| CTCCAATTGTGACAACAACT+AGG | 0.516927 | 4.3:-20720972 | MS.gene068044:CDS |
| AGCTATTGGAATAGGAATCC+TGG | 0.522725 | 4.3:-20720940 | MS.gene068044:CDS |
| AGTTGCAACCTTAGCAGAGA+AGG | 0.536717 | 4.3:-20719957 | MS.gene068044:CDS |
| AGCAGAGATTGTGATTGAAG+GGG | 0.542916 | 4.3:-20720008 | MS.gene068044:CDS |
| GTGGTAAAGAATTGCAGACC+AGG | 0.557976 | 4.3:+20720922 | None:intergenic |
| TGGATAACTTTGAAATTCAG+AGG | 0.566156 | 4.3:-20721057 | MS.gene068044:CDS |
| ACATGTCAACAAGAGGCTCA+TGG | 0.567560 | 4.3:-20721077 | MS.gene068044:CDS |
| AGTAGATTCCAATCAAGCTA+TGG | 0.579189 | 4.3:-20721012 | MS.gene068044:CDS |
| CTAATCAACATGTCAACAAG+AGG | 0.581863 | 4.3:-20721084 | None:intergenic |
| CTTAGCAGAGAAGGTAAGTG+AGG | 0.582808 | 4.3:-20719948 | MS.gene068044:CDS |
| AGATTCCAATCAAGCTATGG+TGG | 0.592764 | 4.3:-20721009 | MS.gene068044:CDS |
| TCCAATTGTGACAACAACTA+GGG | 0.597294 | 4.3:-20720971 | MS.gene068044:CDS |
| TAGTTCATACCAAGAAGAGA+AGG | 0.608523 | 4.3:+20720722 | None:intergenic |
| CAAGCTATGGTGGAGTCAAG+AGG | 0.635710 | 4.3:-20720999 | MS.gene068044:CDS |
| CCATTCTGGAGTCAAAATTG+GGG | 0.671166 | 4.3:-20720182 | MS.gene068044:CDS |
| TGTTGCAGAGACGCTTCCTG+AGG | 0.675670 | 4.3:-20719924 | MS.gene068044:CDS |
| AACAGAAGAATTCATACACA+AGG | 0.676423 | 4.3:-20719831 | MS.gene068044:intron |
| AAAAGTGACTAATGACATGG+AGG | 0.700599 | 4.3:-20719706 | MS.gene068044:CDS |
| TAAGCTAAAGAAAATAGCAG+CGG | 0.709947 | 4.3:-20719897 | MS.gene068044:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCACATAATGATAATTAG+TGG | + | chr4.3:20720959-20720978 | None:intergenic | 20.0% |
| !! | AATCTATAATTTCCATTCAT+TGG | + | chr4.3:20720350-20720369 | None:intergenic | 20.0% |
| !! | AATTAATTATCACTCTCAAA+TGG | + | chr4.3:20720072-20720091 | None:intergenic | 20.0% |
| !! | TAATGAAGTTTATTTGAAGA+TGG | + | chr4.3:20719882-20719901 | None:intergenic | 20.0% |
| !!! | AAGATGGAAATTAAATTTTG+AGG | + | chr4.3:20719866-20719885 | None:intergenic | 20.0% |
| !!! | ACATACATTATTTGATAACT+TGG | - | chr4.3:20720663-20720682 | MS.gene068044:intron | 20.0% |
| !!! | ATCATTATGTGATTAGTTAT+TGG | - | chr4.3:20720965-20720984 | MS.gene068044:CDS | 20.0% |
| !!! | CATACATTATTTGATAACTT+GGG | - | chr4.3:20720664-20720683 | MS.gene068044:intron | 20.0% |
| ! | AGTTATGATTTACCAATGAA+TGG | - | chr4.3:20720335-20720354 | MS.gene068044:intron | 25.0% |
| ! | TAATATCAATGACATACAGA+GGG | - | chr4.3:20720583-20720602 | MS.gene068044:intron | 25.0% |
| ! | TCTAATCATAACAGTAGTAA+GGG | + | chr4.3:20720024-20720043 | None:intergenic | 25.0% |
| ! | TCTTGATCATAATCAACTTA+GGG | + | chr4.3:20720251-20720270 | None:intergenic | 25.0% |
| ! | TTAATATCAATGACATACAG+AGG | - | chr4.3:20720582-20720601 | MS.gene068044:intron | 25.0% |
| ! | TTCACAATCTTGTCAATTAA+GGG | + | chr4.3:20721056-20721075 | None:intergenic | 25.0% |
| ! | TTCTTGATCATAATCAACTT+AGG | + | chr4.3:20720252-20720271 | None:intergenic | 25.0% |
| !! | AATGTTAATAGTGATGATGA+TGG | + | chr4.3:20720934-20720953 | None:intergenic | 25.0% |
| AACAGAAGAATTCATACACA+AGG | - | chr4.3:20720885-20720904 | MS.gene068044:intron | 30.0% | |
| ACATAACATTCAGATATCCT+TGG | + | chr4.3:20720099-20720118 | None:intergenic | 30.0% | |
| CTCTAATCATAACAGTAGTA+AGG | + | chr4.3:20720025-20720044 | None:intergenic | 30.0% | |
| CTTACTACTGTTATGATTAG+AGG | - | chr4.3:20720023-20720042 | MS.gene068044:intron | 30.0% | |
| CTTCACAATCTTGTCAATTA+AGG | + | chr4.3:20721057-20721076 | None:intergenic | 30.0% | |
| GGAAAACTTCAAAGAATAGA+AGG | - | chr4.3:20720555-20720574 | MS.gene068044:intron | 30.0% | |
| GTGATAATTAATTCTCTCCA+AGG | - | chr4.3:20720079-20720098 | MS.gene068044:intron | 30.0% | |
| TAAGCTAAAGAAAATAGCAG+CGG | - | chr4.3:20720819-20720838 | MS.gene068044:intron | 30.0% | |
| TAATCAACTTAGGGTATGTT+TGG | + | chr4.3:20720242-20720261 | None:intergenic | 30.0% | |
| TGAAAAAGTGACTAATGACA+TGG | - | chr4.3:20721007-20721026 | MS.gene068044:CDS | 30.0% | |
| TGATAACTTGGGTTAAAATG+TGG | - | chr4.3:20720675-20720694 | MS.gene068044:intron | 30.0% | |
| TTGAAAGTAGCTATTGGAAT+AGG | - | chr4.3:20719768-20719787 | MS.gene068044:intron | 30.0% | |
| ! | ATTGGTTTCTATTTACCAAC+AGG | - | chr4.3:20720983-20721002 | MS.gene068044:CDS | 30.0% |
| ! | TTAAGTTGTTTGTCCAAACA+TGG | + | chr4.3:20720122-20720141 | None:intergenic | 30.0% |
| !! | TGGATAACTTTGAAATTCAG+AGG | - | chr4.3:20719659-20719678 | MS.gene068044:CDS | 30.0% |
| AAAAGTGACTAATGACATGG+AGG | - | chr4.3:20721010-20721029 | MS.gene068044:CDS | 35.0% | |
| AACAACATGCAGGGTTAAAT+GGG | - | chr4.3:20720482-20720501 | MS.gene068044:intron | 35.0% | |
| AAGCAGAGATTGTGATTGAA+GGG | - | chr4.3:20720707-20720726 | MS.gene068044:intron | 35.0% | |
| AAGGCGAAAAATTTAGAAGC+TGG | + | chr4.3:20719978-20719997 | None:intergenic | 35.0% | |
| AATCATGATGAACAACATGC+AGG | - | chr4.3:20720472-20720491 | MS.gene068044:intron | 35.0% | |
| ACACAAGCACTAATGCAAAT+AGG | - | chr4.3:20719825-20719844 | MS.gene068044:intron | 35.0% | |
| AGGCGAAAAATTTAGAAGCT+GGG | + | chr4.3:20719977-20719996 | None:intergenic | 35.0% | |
| AGTAGATTCCAATCAAGCTA+TGG | - | chr4.3:20719704-20719723 | MS.gene068044:CDS | 35.0% | |
| ATCATGATGAACAACATGCA+GGG | - | chr4.3:20720473-20720492 | MS.gene068044:intron | 35.0% | |
| CAAATAGGTAGCTATACTCA+AGG | - | chr4.3:20719840-20719859 | MS.gene068044:CDS | 35.0% | |
| CTGAATGTTATGTCCATGTT+TGG | - | chr4.3:20720106-20720125 | MS.gene068044:intron | 35.0% | |
| TAGTTCATACCAAGAAGAGA+AGG | + | chr4.3:20719997-20720016 | None:intergenic | 35.0% | |
| TCCAATAACAAGACAGACAT+TGG | - | chr4.3:20720272-20720291 | MS.gene068044:intron | 35.0% | |
| TCCAATTGTGACAACAACTA+GGG | - | chr4.3:20719745-20719764 | MS.gene068044:intron | 35.0% | |
| ! | AATTTTTCGCCTTCTCTTCT+TGG | - | chr4.3:20719985-20720004 | MS.gene068044:CDS | 35.0% |
| ! | ACCAATGTCTGTCTTGTTAT+TGG | + | chr4.3:20720276-20720295 | None:intergenic | 35.0% |
| ! | GTGAATTTTGCAGTTAATCG+TGG | + | chr4.3:20720299-20720318 | None:intergenic | 35.0% |
| !! | ACCATTCTGGAGTCAAAATT+GGG | - | chr4.3:20720533-20720552 | MS.gene068044:intron | 35.0% |
| !! | TACCATTCTGGAGTCAAAAT+TGG | - | chr4.3:20720532-20720551 | MS.gene068044:intron | 35.0% |
| !!! | ATTAGTGCTTGTGTTGCTTT+TGG | + | chr4.3:20719819-20719838 | None:intergenic | 35.0% |
| !!! | TAGTCACTTTTTCAACCTGT+TGG | + | chr4.3:20721001-20721020 | None:intergenic | 35.0% |
| ACCCTAGTTGTTGTCACAAT+TGG | + | chr4.3:20719749-20719768 | None:intergenic | 40.0% | |
| AGATTCCAATCAAGCTATGG+TGG | - | chr4.3:20719707-20719726 | MS.gene068044:CDS | 40.0% | |
| AGCAGAGATTGTGATTGAAG+GGG | - | chr4.3:20720708-20720727 | MS.gene068044:intron | 40.0% | |
| AGCTATTGGAATAGGAATCC+TGG | - | chr4.3:20719776-20719795 | MS.gene068044:intron | 40.0% | |
| ATTTCGCTACGACTCATTAG+AGG | + | chr4.3:20720410-20720429 | None:intergenic | 40.0% | |
| CGTAGCGAAATTTCACACAT+TGG | - | chr4.3:20720419-20720438 | MS.gene068044:intron | 40.0% | |
| CTAGGGTTGAAAGTAGCTAT+TGG | - | chr4.3:20719762-20719781 | MS.gene068044:intron | 40.0% | |
| CTCCAATTGTGACAACAACT+AGG | - | chr4.3:20719744-20719763 | MS.gene068044:intron | 40.0% | |
| GAACAACATGCAGGGTTAAA+TGG | - | chr4.3:20720481-20720500 | MS.gene068044:intron | 40.0% | |
| GAAGCAGAGATTGTGATTGA+AGG | - | chr4.3:20720706-20720725 | MS.gene068044:intron | 40.0% | |
| GATTGAAGGGGTTGAAAAAG+TGG | - | chr4.3:20720720-20720739 | MS.gene068044:intron | 40.0% | |
| GCTTCAAAACAAGCTGTTCA+TGG | - | chr4.3:20720853-20720872 | MS.gene068044:intron | 40.0% | |
| GGCGAAAAATTTAGAAGCTG+GGG | + | chr4.3:20719976-20719995 | None:intergenic | 40.0% | |
| TTGGGTTAAAATGTGGACTC+AGG | - | chr4.3:20720682-20720701 | MS.gene068044:intron | 40.0% | |
| TTTCTTTCGCACTACCATTC+TGG | - | chr4.3:20720520-20720539 | MS.gene068044:intron | 40.0% | |
| ! | TTCTTTAGCTTAGCATCCTC+AGG | + | chr4.3:20720811-20720830 | None:intergenic | 40.0% |
| !! | CCATTCTGGAGTCAAAATTG+GGG | - | chr4.3:20720534-20720553 | MS.gene068044:intron | 40.0% |
| AATTTAGAAGCTGGGGCTTC+AGG | + | chr4.3:20719969-20719988 | None:intergenic | 45.0% | |
| AGTTGCAACCTTAGCAGAGA+AGG | - | chr4.3:20720759-20720778 | MS.gene068044:CDS | 45.0% | |
| CTCACTTACCTTCTCTGCTA+AGG | + | chr4.3:20720770-20720789 | None:intergenic | 45.0% | |
| CTTAGCAGAGAAGGTAAGTG+AGG | - | chr4.3:20720768-20720787 | MS.gene068044:CDS | 45.0% | |
| GTGGTAAAGAATTGCAGACC+AGG | + | chr4.3:20719797-20719816 | None:intergenic | 45.0% | |
| TGACTCCACCATAGCTTGAT+TGG | + | chr4.3:20719715-20719734 | None:intergenic | 45.0% | |
| !!! | AGTGCTTGTGTTGCTTTTGG+TGG | + | chr4.3:20719816-20719835 | None:intergenic | 45.0% |
| !!! | CCCCAATTTTGACTCCAGAA+TGG | + | chr4.3:20720537-20720556 | None:intergenic | 45.0% |
| ! | CAAGCTATGGTGGAGTCAAG+AGG | - | chr4.3:20719717-20719736 | MS.gene068044:intron | 50.0% |
| TGTTGCAGAGACGCTTCCTG+AGG | - | chr4.3:20720792-20720811 | MS.gene068044:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 20719641 | 20721097 | 20719641 | ID=MS.gene068044 |
| chr4.3 | mRNA | 20719641 | 20721097 | 20719641 | ID=MS.gene068044.t1;Parent=MS.gene068044 |
| chr4.3 | exon | 20720892 | 20721097 | 20720892 | ID=MS.gene068044.t1.exon1;Parent=MS.gene068044.t1 |
| chr4.3 | CDS | 20720892 | 20721097 | 20720892 | ID=cds.MS.gene068044.t1;Parent=MS.gene068044.t1 |
| chr4.3 | exon | 20720732 | 20720794 | 20720732 | ID=MS.gene068044.t1.exon2;Parent=MS.gene068044.t1 |
| chr4.3 | CDS | 20720732 | 20720794 | 20720732 | ID=cds.MS.gene068044.t1;Parent=MS.gene068044.t1 |
| chr4.3 | exon | 20720162 | 20720244 | 20720162 | ID=MS.gene068044.t1.exon3;Parent=MS.gene068044.t1 |
| chr4.3 | CDS | 20720162 | 20720244 | 20720162 | ID=cds.MS.gene068044.t1;Parent=MS.gene068044.t1 |
| chr4.3 | exon | 20719832 | 20720034 | 20719832 | ID=MS.gene068044.t1.exon4;Parent=MS.gene068044.t1 |
| chr4.3 | CDS | 20719832 | 20720034 | 20719832 | ID=cds.MS.gene068044.t1;Parent=MS.gene068044.t1 |
| chr4.3 | exon | 20719641 | 20719733 | 20719641 | ID=MS.gene068044.t1.exon5;Parent=MS.gene068044.t1 |
| chr4.3 | CDS | 20719641 | 20719733 | 20719641 | ID=cds.MS.gene068044.t1;Parent=MS.gene068044.t1 |
| Gene Sequence |
| Protein sequence |
