Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068380.t1 | XP_004510647.1 | 100 | 50 | 0 | 0 | 5 | 54 | 63 | 112 | 2.20E-22 | 114 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068380.t1 | O42901 | 51.0 | 49 | 24 | 0 | 6 | 54 | 82 | 130 | 6.8e-09 | 60.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068380.t1 | I3SAF0 | 100.0 | 50 | 0 | 0 | 5 | 54 | 63 | 112 | 1.6e-22 | 114.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05415 | MS.gene068380 | 0.815133 | 1.08E-51 | -1.69E-46 |
| MS.gene054479 | MS.gene068380 | 0.806893 | 6.59E-50 | -1.69E-46 |
| MS.gene055724 | MS.gene068380 | 0.815803 | 7.67E-52 | -1.69E-46 |
| MS.gene056174 | MS.gene068380 | 0.8029 | 4.50E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene068380 | 0.803769 | 2.98E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene068380 | 0.803037 | 4.22E-49 | -1.69E-46 |
| MS.gene060777 | MS.gene068380 | 0.80129 | 9.65E-49 | -1.69E-46 |
| MS.gene060937 | MS.gene068380 | 0.800237 | 1.58E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068380.t1 | MTR_8g027285 | 100.000 | 50 | 0 | 0 | 5 | 54 | 63 | 112 | 2.55e-32 | 108 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068380.t1 | AT5G42000 | 90.000 | 50 | 5 | 0 | 5 | 54 | 60 | 109 | 1.39e-29 | 100 |
| MS.gene068380.t1 | AT5G42000 | 90.000 | 50 | 5 | 0 | 5 | 54 | 60 | 109 | 2.37e-29 | 100 |
| MS.gene068380.t1 | AT1G01230 | 86.000 | 50 | 7 | 0 | 5 | 54 | 63 | 112 | 3.24e-27 | 95.9 |
Find 15 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTCCATTTGCAGATGATC+AGG | 0.373395 | 8.3:-67101574 | MS.gene068380:CDS |
| GAAAGCAGCTCACTCGTAAC+AGG | 0.409598 | 8.3:-67101509 | MS.gene068380:CDS |
| ATCCCCTGATCATCTGCAAA+TGG | 0.508493 | 8.3:+67101570 | None:intergenic |
| TAGATTGACATGGTGGGAGC+AGG | 0.523259 | 8.3:-67101541 | MS.gene068380:CDS |
| ATGGAGTTCCTTTCTTCCAG+TGG | 0.534161 | 8.3:+67101589 | None:intergenic |
| ACTCCATTTGCAGATGATCA+GGG | 0.556480 | 8.3:-67101573 | MS.gene068380:CDS |
| TGTAGCATTTAATGAGGCGA+GGG | 0.574458 | 8.3:-67101764 | None:intergenic |
| TGGTGGGAGCAGGTCGACAA+TGG | 0.574609 | 8.3:-67101531 | MS.gene068380:CDS |
| AGGAGAACTTACAGAACCAA+AGG | 0.601770 | 8.3:+67101468 | None:intergenic |
| ATGTAGCATTTAATGAGGCG+AGG | 0.605289 | 8.3:-67101765 | None:intergenic |
| CTCCATTTGCAGATGATCAG+GGG | 0.621400 | 8.3:-67101572 | MS.gene068380:CDS |
| TCTACAATAGATTGACATGG+TGG | 0.624727 | 8.3:-67101548 | MS.gene068380:CDS |
| CTACAATAGATTGACATGGT+GGG | 0.646152 | 8.3:-67101547 | MS.gene068380:CDS |
| GTAGCATTTAATGAGGCGAG+GGG | 0.708569 | 8.3:-67101763 | None:intergenic |
| GGATCTACAATAGATTGACA+TGG | 0.711938 | 8.3:-67101551 | MS.gene068380:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTATAAAAGCTACTTGACT+GGG | - | chr8.3:67101500-67101519 | MS.gene068380:CDS | 25.0% |
| !! | GTTAATTTGATCTCTTTACA+TGG | - | chr8.3:67101574-67101593 | MS.gene068380:CDS | 25.0% |
| !! | TTTTATAAAAGCTACTTGAC+TGG | - | chr8.3:67101499-67101518 | MS.gene068380:CDS | 25.0% |
| !!! | TAACATATCACTTTTTCCAC+TGG | - | chr8.3:67101624-67101643 | MS.gene068380:intron | 30.0% |
| ATTGATATGTTGTGTGCTTC+AGG | - | chr8.3:67101601-67101620 | MS.gene068380:CDS | 35.0% | |
| CTACAATAGATTGACATGGT+GGG | - | chr8.3:67101682-67101701 | MS.gene068380:intron | 35.0% | |
| GGATCTACAATAGATTGACA+TGG | - | chr8.3:67101678-67101697 | MS.gene068380:intron | 35.0% | |
| TCTACAATAGATTGACATGG+TGG | - | chr8.3:67101681-67101700 | MS.gene068380:intron | 35.0% | |
| ! | GTTTTTGACAGTTGTTCCTT+TGG | - | chr8.3:67101745-67101764 | MS.gene068380:intron | 35.0% |
| AACTCCATTTGCAGATGATC+AGG | - | chr8.3:67101655-67101674 | MS.gene068380:intron | 40.0% | |
| ACTCCATTTGCAGATGATCA+GGG | - | chr8.3:67101656-67101675 | MS.gene068380:intron | 40.0% | |
| !!! | CACTTTTTCCACTGGAAGAA+AGG | - | chr8.3:67101632-67101651 | MS.gene068380:intron | 40.0% |
| ATCCCCTGATCATCTGCAAA+TGG | + | chr8.3:67101662-67101681 | None:intergenic | 45.0% | |
| ATGGAGTTCCTTTCTTCCAG+TGG | + | chr8.3:67101643-67101662 | None:intergenic | 45.0% | |
| CTCCATTTGCAGATGATCAG+GGG | - | chr8.3:67101657-67101676 | MS.gene068380:intron | 45.0% | |
| GAAAGCAGCTCACTCGTAAC+AGG | - | chr8.3:67101720-67101739 | MS.gene068380:intron | 50.0% | |
| ! | TAGATTGACATGGTGGGAGC+AGG | - | chr8.3:67101688-67101707 | MS.gene068380:intron | 50.0% |
| TGGTGGGAGCAGGTCGACAA+TGG | - | chr8.3:67101698-67101717 | MS.gene068380:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 67101476 | 67101775 | 67101476 | ID=MS.gene068380 |
| chr8.3 | mRNA | 67101476 | 67101775 | 67101476 | ID=MS.gene068380.t1;Parent=MS.gene068380 |
| chr8.3 | exon | 67101764 | 67101775 | 67101764 | ID=MS.gene068380.t1.exon1;Parent=MS.gene068380.t1 |
| chr8.3 | CDS | 67101764 | 67101775 | 67101764 | ID=cds.MS.gene068380.t1;Parent=MS.gene068380.t1 |
| chr8.3 | exon | 67101476 | 67101628 | 67101476 | ID=MS.gene068380.t1.exon2;Parent=MS.gene068380.t1 |
| chr8.3 | CDS | 67101476 | 67101628 | 67101476 | ID=cds.MS.gene068380.t1;Parent=MS.gene068380.t1 |
| Gene Sequence |
| Protein sequence |
