Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068682.t1 | RHN38568.1 | 91.4 | 197 | 4 | 3 | 1 | 193 | 858 | 1045 | 6.90E-87 | 330.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068682.t1 | A5YKK6 | 47.1 | 102 | 53 | 1 | 92 | 193 | 1074 | 1174 | 2.5e-21 | 103.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068682.t1 | G7LAR7 | 91.4 | 197 | 4 | 3 | 1 | 193 | 850 | 1037 | 5.0e-87 | 330.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055784 | MS.gene068682 | 0.80291 | 4.48E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068682.t1 | MTR_8g005820 | 91.371 | 197 | 4 | 3 | 1 | 193 | 850 | 1037 | 1.67e-113 | 355 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068682.t1 | AT1G02080 | 61.340 | 194 | 64 | 3 | 1 | 193 | 844 | 1027 | 3.19e-67 | 223 |
| MS.gene068682.t1 | AT1G02080 | 61.340 | 194 | 64 | 3 | 1 | 193 | 844 | 1027 | 3.35e-67 | 223 |
| MS.gene068682.t1 | AT1G02080 | 61.340 | 194 | 64 | 3 | 1 | 193 | 898 | 1081 | 3.55e-67 | 223 |
Find 0 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATCATATAATATAAAGAGA+AGG | - | 56121:11836-11855 | MS.gene068682:intergenic | 15.0% |
| !!! | TTATACAAATTGATTCTTTT+TGG | + | 56121:11886-11905 | MS.gene068682:intron | 15.0% |
| !! | TATTATTACTTGTAGTGTTT+CGG | + | 56121:10804-10823 | MS.gene068682:intron | 20.0% |
| !! | TCATAAGCACAAAATTAAAA+CGG | - | 56121:11178-11197 | MS.gene068682:intergenic | 20.0% |
| ! | AACAATCAATTTCTTGCAAA+TGG | - | 56121:11124-11143 | MS.gene068682:intergenic | 25.0% |
| ! | AACCAAAGTTTCAATATTCA+AGG | - | 56121:11059-11078 | MS.gene068682:intergenic | 25.0% |
| ! | AATGTAAACTTATGTACATG+GGG | + | 56121:11517-11536 | MS.gene068682:intron | 25.0% |
| ! | CAATGTAAACTTATGTACAT+GGG | + | 56121:11516-11535 | MS.gene068682:intron | 25.0% |
| ! | GCTGATAATAAGTATAGTAT+GGG | + | 56121:11549-11568 | MS.gene068682:intron | 25.0% |
| ! | TACTTTATGTTATACTGTAC+TGG | + | 56121:11618-11637 | MS.gene068682:intron | 25.0% |
| ! | TCAATGTAAACTTATGTACA+TGG | + | 56121:11515-11534 | MS.gene068682:intron | 25.0% |
| ! | TGCTGATAATAAGTATAGTA+TGG | + | 56121:11548-11567 | MS.gene068682:intron | 25.0% |
| !!! | AATTCCTTTTGATTACTCAA+GGG | + | 56121:10945-10964 | MS.gene068682:intron | 25.0% |
| !!! | ATAATTGAGTTTTATATCCC+CGG | - | 56121:11664-11683 | MS.gene068682:intergenic | 25.0% |
| AAAAGACAGAAATAGAGATG+GGG | - | 56121:10782-10801 | None:intergenic | 30.0% | |
| ACAACTCATTAAACTGACAT+AGG | - | 56121:11235-11254 | None:intergenic | 30.0% | |
| ATCAGTTCAGTTAATCTTGA+AGG | - | 56121:11744-11763 | MS.gene068682:intergenic | 30.0% | |
| ATCATGCTCAAAAAACATGA+TGG | - | 56121:10742-10761 | MS.gene068682:intergenic | 30.0% | |
| CAAAAGACAGAAATAGAGAT+GGG | - | 56121:10783-10802 | MS.gene068682:intergenic | 30.0% | |
| TAACTTTGTCCAGAAATTTC+AGG | - | 56121:11948-11967 | MS.gene068682:intergenic | 30.0% | |
| TAATAACTGATCTGTTGACA+TGG | + | 56121:11767-11786 | MS.gene068682:intron | 30.0% | |
| TAATATCGAAGCCAAATCAA+AGG | + | 56121:11376-11395 | MS.gene068682:CDS | 30.0% | |
| TCTGGACAAAGTTAATTCAA+AGG | + | 56121:11954-11973 | MS.gene068682:CDS | 30.0% | |
| TCTTTGTCTTTGCCATAAAT+AGG | + | 56121:11295-11314 | MS.gene068682:intron | 30.0% | |
| TGCCTTGAATATTGAAACTT+TGG | + | 56121:11054-11073 | MS.gene068682:CDS | 30.0% | |
| TTATAAACGATATCTTGTCC+TGG | - | 56121:11337-11356 | MS.gene068682:intergenic | 30.0% | |
| ! | ATGGAAGTATAGTACCTTAT+TGG | - | 56121:10715-10734 | MS.gene068682:intergenic | 30.0% |
| ! | CAAAAAACATGATGGAAGTA+TGG | - | 56121:10734-10753 | MS.gene068682:intergenic | 30.0% |
| ! | GGAAATAGGACTATTTTACA+TGG | + | 56121:11788-11807 | MS.gene068682:intron | 30.0% |
| !! | CAATTCCTTTTGATTACTCA+AGG | + | 56121:10944-10963 | MS.gene068682:intron | 30.0% |
| !!! | TTTTTTGAGCATGATGTGAT+TGG | + | 56121:10747-10766 | MS.gene068682:intron | 30.0% |
| AAAGACAGAAATAGAGATGG+GGG | - | 56121:10781-10800 | None:intergenic | 35.0% | |
| AAGAAGATGATAACATTGGC+TGG | - | 56121:10854-10873 | None:intergenic | 35.0% | |
| ACAGAGACGTGAAAATCATT+TGG | + | 56121:10587-10606 | MS.gene068682:CDS | 35.0% | |
| AGGTACATGTCATGGAAATT+CGG | - | 56121:11928-11947 | MS.gene068682:intergenic | 35.0% | |
| ATGTTATCATCTTCTTCTCC+TGG | + | 56121:10858-10877 | MS.gene068682:CDS | 35.0% | |
| CACAAGTATCAGAAACATAC+TGG | - | 56121:10922-10941 | MS.gene068682:intergenic | 35.0% | |
| CCAAAAGACAGAAATAGAGA+TGG | - | 56121:10784-10803 | MS.gene068682:intergenic | 35.0% | |
| GAAAAGAGAAACTCCTATTG+AGG | + | 56121:11090-11109 | MS.gene068682:CDS | 35.0% | |
| GGAGAAGAAGATGATAACAT+TGG | - | 56121:10858-10877 | MS.gene068682:intergenic | 35.0% | |
| TATAGTACCTTATTGGCACT+AGG | - | 56121:10708-10727 | MS.gene068682:intergenic | 35.0% | |
| TATGCCCTTGAGTAATCAAA+AGG | - | 56121:10952-10971 | MS.gene068682:intergenic | 35.0% | |
| TGACATGTACCTGAAATTTC+TGG | + | 56121:11936-11955 | MS.gene068682:CDS | 35.0% | |
| TGATCTGTTGACATGGAAAT+AGG | + | 56121:11774-11793 | MS.gene068682:intron | 35.0% | |
| TTTGAACAAAGAGATTGTCC+AGG | + | 56121:11978-11997 | MS.gene068682:CDS | 35.0% | |
| ! | ACCTTTTCATGACCATATAC+TGG | - | 56121:11445-11464 | MS.gene068682:intergenic | 35.0% |
| ! | CCTTTTCATGACCATATACT+GGG | - | 56121:11444-11463 | MS.gene068682:intergenic | 35.0% |
| ! | CTGTTGATTGATGTAGAGTT+TGG | + | 56121:11028-11047 | MS.gene068682:intron | 35.0% |
| ! | GAAATTTCAGGTACATGTCA+TGG | - | 56121:11936-11955 | MS.gene068682:intergenic | 35.0% |
| ! | GGGTTTCATAGAGATTAGTT+CGG | + | 56121:11569-11588 | MS.gene068682:intron | 35.0% |
| ! | TACTCAAGGGCATAAGTATT+GGG | + | 56121:10958-10977 | MS.gene068682:intron | 35.0% |
| ! | TTACTCAAGGGCATAAGTAT+TGG | + | 56121:10957-10976 | MS.gene068682:intron | 35.0% |
| ! | TTTGGCTTCGATATTAGTAG+AGG | - | 56121:11372-11391 | MS.gene068682:intergenic | 35.0% |
| !!! | CCATCTCTATTTCTGTCTTT+TGG | + | 56121:10781-10800 | MS.gene068682:intron | 35.0% |
| CCCAGTATATGGTCATGAAA+AGG | + | 56121:11441-11460 | MS.gene068682:CDS | 40.0% | |
| GATTGCCCTATAGAAGCTAA+TGG | - | 56121:10660-10679 | MS.gene068682:intergenic | 40.0% | |
| GTGTTTATCAGTGTCTGATC+CGG | + | 56121:11642-11661 | MS.gene068682:intron | 40.0% | |
| TGAAAGAGCAGTACTATCCA+TGG | + | 56121:11414-11433 | MS.gene068682:CDS | 40.0% | |
| TGTTTATCAGTGTCTGATCC+GGG | + | 56121:11643-11662 | MS.gene068682:intron | 40.0% | |
| TTCTGTGAGCTCCTTTGATT+TGG | - | 56121:11390-11409 | MS.gene068682:intergenic | 40.0% | |
| ! | AAGCCACCATTAGCTTCTAT+AGG | + | 56121:10651-10670 | MS.gene068682:CDS | 40.0% |
| ! | AGCCACCATTAGCTTCTATA+GGG | + | 56121:10652-10671 | MS.gene068682:CDS | 40.0% |
| ! | TCATAGAGATTAGTTCGGCA+AGG | + | 56121:11574-11593 | MS.gene068682:intron | 40.0% |
| ! | TGCAATTTTCGTATGTTGCC+TGG | - | 56121:11999-12018 | MS.gene068682:intergenic | 40.0% |
| ACCATATACTGGGCAAACCA+TGG | - | 56121:11434-11453 | MS.gene068682:intergenic | 45.0% | |
| CTGATCCTGGAGCCTATTTA+TGG | - | 56121:11310-11329 | MS.gene068682:intergenic | 45.0% | |
| CTTTGCCATAAATAGGCTCC+AGG | + | 56121:11302-11321 | MS.gene068682:intron | 45.0% | |
| GATCGTCAGAAGACATCTGT+TGG | + | 56121:10612-10631 | MS.gene068682:CDS | 45.0% | |
| GTTTATCAGTGTCTGATCCG+GGG | + | 56121:11644-11663 | MS.gene068682:intron | 45.0% | |
| TAAATAGGCTCCAGGATCAG+AGG | + | 56121:11310-11329 | MS.gene068682:intron | 45.0% | |
| TACTGGTAGAAGCAGATCCA+CGG | - | 56121:10905-10924 | MS.gene068682:intergenic | 45.0% | |
| TGCCCTATAGAAGCTAATGG+TGG | - | 56121:10657-10676 | MS.gene068682:intergenic | 45.0% | |
| ! | AATGGCTAGAGCACCTCAAT+AGG | - | 56121:11106-11125 | MS.gene068682:intergenic | 45.0% |
| ! | CTTATTGGCACTAGGAGCAT+CGG | - | 56121:10700-10719 | MS.gene068682:intergenic | 45.0% |
| ! | TCCATGGTTTGCCCAGTATA+TGG | + | 56121:11430-11449 | MS.gene068682:CDS | 45.0% |
| ACTGGTAGAAGCAGATCCAC+GGG | - | 56121:10904-10923 | MS.gene068682:intergenic | 50.0% | |
| AGTGACATATGCTGCTGTCC+AGG | - | 56121:10558-10577 | MS.gene068682:intergenic | 50.0% | |
| CGATGCTCCTAGTGCCAATA+AGG | + | 56121:10698-10717 | MS.gene068682:CDS | 50.0% | |
| GTAGAAGCAGATCCACGGGA+CGG | - | 56121:10900-10919 | MS.gene068682:intergenic | 55.0% | |
| GTCCAGGTTGTGTGATGCCA+GGG | - | 56121:10542-10561 | MS.gene068682:intergenic | 55.0% | |
| TAGAAGCAGATCCACGGGAC+GGG | - | 56121:10899-10918 | MS.gene068682:intergenic | 55.0% | |
| TGTCCAGGTTGTGTGATGCC+AGG | - | 56121:10543-10562 | MS.gene068682:intergenic | 55.0% | |
| TTGTCCTGGACCTCTGATCC+TGG | - | 56121:11323-11342 | MS.gene068682:intergenic | 55.0% | |
| ! | GCATCGGTCGGTGCAATTAC+TGG | - | 56121:10684-10703 | MS.gene068682:intergenic | 55.0% |
| !! | TTGGCACTAGGAGCATCGGT+CGG | - | 56121:10696-10715 | MS.gene068682:intergenic | 55.0% |
| AGAAGCAGATCCACGGGACG+GGG | - | 56121:10898-10917 | MS.gene068682:intergenic | 60.0% | |
| GGCCCTGGCATCACACAACC+TGG | + | 56121:10537-10556 | MS.gene068682:CDS | 65.0% | |
| GGCTCCAGGATCAGAGGTCC+AGG | + | 56121:11316-11335 | MS.gene068682:intron | 65.0% | |
| GGGGACGGACGGACAAAACC+AGG | - | 56121:10879-10898 | MS.gene068682:intergenic | 65.0% | |
| ATCCACGGGACGGGGACGGA+CGG | - | 56121:10890-10909 | MS.gene068682:intergenic | 70.0% | |
| GCAGATCCACGGGACGGGGA+CGG | - | 56121:10894-10913 | MS.gene068682:intergenic | 70.0% | |
| ! | GTCCGTCCGTCCCCGTCCCG+TGG | + | 56121:10885-10904 | MS.gene068682:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 56121 | gene | 10531 | 12020 | 10531 | ID=MS.gene068682 |
| 56121 | mRNA | 10531 | 12020 | 10531 | ID=MS.gene068682.t1;Parent=MS.gene068682 |
| 56121 | exon | 10531 | 10719 | 10531 | ID=MS.gene068682.t1.exon1;Parent=MS.gene068682.t1 |
| 56121 | CDS | 10531 | 10719 | 10531 | ID=cds.MS.gene068682.t1;Parent=MS.gene068682.t1 |
| 56121 | exon | 10855 | 10921 | 10855 | ID=MS.gene068682.t1.exon2;Parent=MS.gene068682.t1 |
| 56121 | CDS | 10855 | 10921 | 10855 | ID=cds.MS.gene068682.t1;Parent=MS.gene068682.t1 |
| 56121 | exon | 11044 | 11111 | 11044 | ID=MS.gene068682.t1.exon3;Parent=MS.gene068682.t1 |
| 56121 | CDS | 11044 | 11111 | 11044 | ID=cds.MS.gene068682.t1;Parent=MS.gene068682.t1 |
| 56121 | exon | 11317 | 11462 | 11317 | ID=MS.gene068682.t1.exon4;Parent=MS.gene068682.t1 |
| 56121 | CDS | 11317 | 11462 | 11317 | ID=cds.MS.gene068682.t1;Parent=MS.gene068682.t1 |
| 56121 | exon | 11912 | 12020 | 11912 | ID=MS.gene068682.t1.exon5;Parent=MS.gene068682.t1 |
| 56121 | CDS | 11912 | 12020 | 11912 | ID=cds.MS.gene068682.t1;Parent=MS.gene068682.t1 |
| Gene Sequence |
| Protein sequence |
