Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068802.t1 | XP_013467939.1 | 68.9 | 270 | 59 | 8 | 1 | 253 | 1 | 262 | 5.30E-79 | 304.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068802.t1 | Q9SIH5 | 41.4 | 251 | 118 | 8 | 13 | 253 | 14 | 245 | 9.0e-35 | 148.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068802.t1 | A0A072VK35 | 68.9 | 270 | 59 | 8 | 1 | 253 | 1 | 262 | 3.8e-79 | 304.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056857 | MS.gene068802 | 0.834426 | 3.02E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068802.t1 | MTR_1g058490 | 69.373 | 271 | 56 | 9 | 1 | 253 | 1 | 262 | 1.54e-109 | 319 |
| MS.gene068802.t1 | MTR_7g105320 | 42.403 | 283 | 115 | 8 | 1 | 253 | 2 | 266 | 2.18e-55 | 181 |
| MS.gene068802.t1 | MTR_1g060460 | 64.238 | 151 | 32 | 3 | 1 | 149 | 27 | 157 | 2.31e-51 | 165 |
| MS.gene068802.t1 | MTR_2g086920 | 32.558 | 258 | 148 | 8 | 14 | 253 | 3 | 252 | 5.70e-30 | 114 |
| MS.gene068802.t1 | MTR_4g052460 | 34.496 | 258 | 139 | 7 | 19 | 252 | 24 | 275 | 4.91e-27 | 107 |
| MS.gene068802.t1 | MTR_3g466350 | 31.873 | 251 | 145 | 11 | 16 | 245 | 6 | 251 | 3.78e-19 | 84.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068802.t1 | AT2G36090 | 42.063 | 252 | 115 | 9 | 13 | 253 | 14 | 245 | 9.02e-46 | 155 |
| MS.gene068802.t1 | AT2G27310 | 37.262 | 263 | 136 | 9 | 15 | 253 | 11 | 268 | 7.15e-38 | 135 |
| MS.gene068802.t1 | AT3G44326 | 36.742 | 264 | 130 | 8 | 17 | 251 | 22 | 277 | 5.71e-37 | 134 |
Find 0 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTTACAATGGAAAATATCAA+CGG | - | 3511:464-483 | MS.gene068802:intergenic | 25.0% |
| !! | GTTGATATTTTCCATTGTAA+GGG | + | 3511:463-482 | MS.gene068802:CDS | 25.0% |
| AAAACACTAAACCCTTACAA+TGG | - | 3511:477-496 | MS.gene068801:intergenic | 30.0% | |
| TATCTCTTCTCATCATCAAA+GGG | + | 3511:394-413 | MS.gene068802:CDS | 30.0% | |
| ! | CGTTGATATTTTCCATTGTA+AGG | + | 3511:462-481 | MS.gene068802:CDS | 30.0% |
| AACGGCTGAGATTAAACTTA+GGG | - | 3511:446-465 | MS.gene068802:intergenic | 35.0% | |
| CTATCTCTTCTCATCATCAA+AGG | + | 3511:393-412 | MS.gene068802:CDS | 35.0% | |
| CTGAGATTAAACTTAGGGTT+CGG | - | 3511:441-460 | MS.gene068802:intergenic | 35.0% | |
| GATGTGATCAAATATCCAGT+TGG | + | 3511:574-593 | MS.gene068802:CDS | 35.0% | |
| TGTTGACCTACTAGATAACA+AGG | + | 3511:552-571 | MS.gene068802:CDS | 35.0% | |
| ! | TCACATCCTTGTTATCTAGT+AGG | - | 3511:561-580 | MS.gene068802:intergenic | 35.0% |
| ATGGAAAACACGTGAATGGA+AGG | + | 3511:851-870 | MS.gene068802:CDS | 40.0% | |
| ATGGATGGAAAACACGTGAA+TGG | + | 3511:847-866 | MS.gene068802:CDS | 40.0% | |
| CAACGGCTGAGATTAAACTT+AGG | - | 3511:447-466 | MS.gene068802:intergenic | 40.0% | |
| GTGATCAAATATCCAGTTGG+TGG | + | 3511:577-596 | MS.gene068802:CDS | 40.0% | |
| TCTAGTAGGTCAACACGAAA+CGG | - | 3511:547-566 | MS.gene068802:intergenic | 40.0% | |
| TGGAAAACACGTGAATGGAA+GGG | + | 3511:852-871 | MS.gene068802:CDS | 40.0% | |
| ! | TAGTTTGCAAGTGGAGGATA+TGG | + | 3511:828-847 | MS.gene068802:CDS | 40.0% |
| !! | TAGAGAGGTTAGTTTGCAAG+TGG | + | 3511:819-838 | MS.gene068802:CDS | 40.0% |
| !! | TTGGTGGTGATGAAAACCTA+GGG | + | 3511:593-612 | MS.gene068802:CDS | 40.0% |
| ACGTGAATGGAAGGGATAGT+TGG | + | 3511:860-879 | MS.gene068802:CDS | 45.0% | |
| CAAACGCATGCTATTCTACG+TGG | + | 3511:294-313 | MS.gene068802:CDS | 45.0% | |
| CGGAGAACGTTGAGATTACA+TGG | - | 3511:346-365 | MS.gene068802:intergenic | 45.0% | |
| CTGTTTGCAACGGTGGTTTA+CGG | + | 3511:727-746 | MS.gene068802:CDS | 45.0% | |
| GGAACAGAGAGAGTTGAGTT+GGG | - | 3511:257-276 | MS.gene068802:intergenic | 45.0% | |
| GGAGAATATGGAGGTTAGAG+AGG | + | 3511:804-823 | MS.gene068802:CDS | 45.0% | |
| GTAATCTCAACGTTCTCCGA+TGG | + | 3511:347-366 | MS.gene068802:CDS | 45.0% | |
| TAGAGTCTGCGAAGAAAGAG+CGG | - | 3511:376-395 | MS.gene068802:intergenic | 45.0% | |
| TGTTCCGACGAAACATCTCT+GGG | + | 3511:271-290 | MS.gene068802:CDS | 45.0% | |
| TTGCAAGTGGAGGATATGGA+TGG | + | 3511:832-851 | MS.gene068802:CDS | 45.0% | |
| TTGGATTATGTCGTCGTGGA+CGG | - | 3511:176-195 | MS.gene068802:intergenic | 45.0% | |
| TTTGCCCAGAGATGTTTCGT+CGG | - | 3511:278-297 | MS.gene068802:intergenic | 45.0% | |
| ! | AGGTTTTCATCACCACCAAC+TGG | - | 3511:592-611 | MS.gene068802:intergenic | 45.0% |
| ! | TGGTGGTGATGAAAACCTAG+GGG | + | 3511:594-613 | MS.gene068802:CDS | 45.0% |
| !! | AGAGGTTAGTTTGCAAGTGG+AGG | + | 3511:822-841 | MS.gene068802:CDS | 45.0% |
| !! | GAGCTGGATTTTGATCGATC+CGG | + | 3511:630-649 | MS.gene068802:CDS | 45.0% |
| !! | GGTGAAGTTGCTGTTTGCAA+CGG | + | 3511:717-736 | MS.gene068802:CDS | 45.0% |
| !! | GTTGGTGGTGATGAAAACCT+AGG | + | 3511:592-611 | MS.gene068802:CDS | 45.0% |
| AAAACCTAGGGGAGGAGTTG+AGG | + | 3511:605-624 | MS.gene068802:CDS | 50.0% | |
| AAACTGAAACCGTGACCACC+CGG | + | 3511:509-528 | MS.gene068802:CDS | 50.0% | |
| AGAGTCTGCGAAGAAAGAGC+GGG | - | 3511:375-394 | MS.gene068802:intergenic | 50.0% | |
| CACATACTCACACGCCTTGA+CGG | + | 3511:199-218 | MS.gene068802:CDS | 50.0% | |
| CGGAACAGAGAGAGTTGAGT+TGG | - | 3511:258-277 | MS.gene068802:intergenic | 50.0% | |
| CTGTTCCGACGAAACATCTC+TGG | + | 3511:270-289 | MS.gene068802:CDS | 50.0% | |
| GAGCTTGGATTATGTCGTCG+TGG | - | 3511:180-199 | MS.gene068802:intergenic | 50.0% | |
| GATGTTGGCGGAGAATATGG+AGG | + | 3511:795-814 | MS.gene068802:CDS | 50.0% | |
| GGTGATGTTGGCGGAGAATA+TGG | + | 3511:792-811 | MS.gene068802:CDS | 50.0% | |
| GGTTTACGGAGAAACAGCGA+CGG | + | 3511:741-760 | MS.gene068802:CDS | 50.0% | |
| TGATCGATCCGGTGAGTCAA+CGG | + | 3511:641-660 | MS.gene068802:CDS | 50.0% | |
| TTGCATTTCCGGCGAATCAG+AGG | + | 3511:126-145 | MS.gene068802:CDS | 50.0% | |
| ! | GTGTAGTGTGGTGGTGATGT+TGG | + | 3511:780-799 | MS.gene068802:CDS | 50.0% |
| ! | TAGTGTGGTGGTGATGTTGG+CGG | + | 3511:783-802 | MS.gene068802:CDS | 50.0% |
| ! | TGGTGATGAAAACCTAGGGG+AGG | + | 3511:597-616 | MS.gene068802:CDS | 50.0% |
| !! | GAAGTTGCTGTTTGCAACGG+TGG | + | 3511:720-739 | MS.gene068802:CDS | 50.0% |
| CATTTCCGGCGAATCAGAGG+AGG | + | 3511:129-148 | MS.gene068802:CDS | 55.0% | |
| GAAGAAAGAGCGGGAGCCAT+CGG | - | 3511:366-385 | MS.gene068802:intergenic | 55.0% | |
| GAGATTACATGGCGAACCCG+TGG | - | 3511:335-354 | MS.gene068802:intergenic | 55.0% | |
| GATCGATCCGGTGAGTCAAC+GGG | + | 3511:642-661 | MS.gene068802:CDS | 55.0% | |
| GATTATGTCGTCGTGGACGG+CGG | - | 3511:173-192 | MS.gene068802:intergenic | 55.0% | |
| GGCGTGTGAGTATGTGAGCT+TGG | - | 3511:195-214 | MS.gene068802:intergenic | 55.0% | |
| ! | CAACACGAAACGGAGAGCAC+CGG | - | 3511:537-556 | MS.gene068802:intergenic | 55.0% |
| ! | TTCAGCCTCCTCTGATTCGC+CGG | - | 3511:137-156 | MS.gene068802:intergenic | 55.0% |
| !! | GAGAGAGTTGAGTTGGGAGC+AGG | - | 3511:251-270 | MS.gene068802:intergenic | 55.0% |
| AAACCGTGACCACCCGGTTC+CGG | + | 3511:515-534 | MS.gene068802:CDS | 60.0% | |
| GAACGTGTCGAGTGGCAAGG+CGG | + | 3511:669-688 | MS.gene068802:CDS | 60.0% | |
| GAGTTGGGAGCAGGTGCTTG+CGG | - | 3511:242-261 | MS.gene068802:intergenic | 60.0% | |
| GGAGGTGGCGTTGTGTAGTG+TGG | + | 3511:768-787 | MS.gene068802:CDS | 60.0% | |
| GGTGAACGTGTCGAGTGGCA+AGG | + | 3511:666-685 | MS.gene068802:CDS | 60.0% | |
| GGTGGCGTTGTGTAGTGTGG+TGG | + | 3511:771-790 | MS.gene068802:CDS | 60.0% | |
| TCAGCCTCAACTCCTCCCCT+AGG | - | 3511:612-631 | MS.gene068802:intergenic | 60.0% | |
| !! | AAACGGAGAGCACCGGAACC+GGG | - | 3511:530-549 | MS.gene068802:intergenic | 60.0% |
| !! | GAAACGGAGAGCACCGGAAC+CGG | - | 3511:531-550 | MS.gene068802:intergenic | 60.0% |
| !! | TTCACCGCCCGTTGACTCAC+CGG | - | 3511:652-671 | MS.gene068802:intergenic | 60.0% |
| AGAAACAGCGACGGCGACGG+AGG | + | 3511:750-769 | MS.gene068802:CDS | 65.0% | |
| ATGGCGAACCCGTGGCGTGT+TGG | - | 3511:327-346 | MS.gene068802:intergenic | 65.0% | |
| CGATCCGGTGAGTCAACGGG+CGG | + | 3511:645-664 | MS.gene068802:CDS | 65.0% | |
| CGGAGAAACAGCGACGGCGA+CGG | + | 3511:747-766 | MS.gene068802:CDS | 65.0% | |
| GCCTTCCACCAACACGCCAC+GGG | + | 3511:316-335 | MS.gene068802:CDS | 65.0% | |
| GGCCTTCCACCAACACGCCA+CGG | + | 3511:315-334 | MS.gene068802:CDS | 65.0% | |
| GGGAGGAGTTGAGGCTGAGC+TGG | + | 3511:614-633 | MS.gene068802:CDS | 65.0% | |
| GTCGTGGACGGCGGAGAATG+TGG | - | 3511:164-183 | MS.gene068802:intergenic | 65.0% | |
| TGGAGGTGGTGCAGCGGAGT+GGG | + | 3511:692-711 | MS.gene068802:CDS | 65.0% | |
| TTGGGAGCAGGTGCTTGCGG+CGG | - | 3511:239-258 | MS.gene068802:intergenic | 65.0% | |
| ! | ACCCGTGGCGTGTTGGTGGA+AGG | - | 3511:320-339 | MS.gene068802:intergenic | 65.0% |
| AACAGCGACGGCGACGGAGG+TGG | + | 3511:753-772 | MS.gene068802:CDS | 70.0% | |
| AAGCGAGAGCGGCGCCGTCA+AGG | - | 3511:216-235 | MS.gene068802:intergenic | 70.0% | |
| AGGCGGTGGAGGTGGTGCAG+CGG | + | 3511:686-705 | MS.gene068802:CDS | 70.0% | |
| CGGGCGGTGAACGTGTCGAG+TGG | + | 3511:661-680 | MS.gene068802:CDS | 70.0% | |
| CGTGTCGAGTGGCAAGGCGG+TGG | + | 3511:672-691 | MS.gene068802:CDS | 70.0% | |
| GAGTGGCAAGGCGGTGGAGG+TGG | + | 3511:678-697 | MS.gene068802:CDS | 70.0% | |
| GCACCGGAACCGGGTGGTCA+CGG | - | 3511:521-540 | MS.gene068802:intergenic | 70.0% | |
| GCGAACCCGTGGCGTGTTGG+TGG | - | 3511:324-343 | MS.gene068802:intergenic | 70.0% | |
| GCTTGCGGCGGAAGCGAGAG+CGG | - | 3511:227-246 | MS.gene068802:intergenic | 70.0% | |
| GGAGGTGGTGCAGCGGAGTG+GGG | + | 3511:693-712 | MS.gene068802:CDS | 70.0% | |
| GTCGAGTGGCAAGGCGGTGG+AGG | + | 3511:675-694 | MS.gene068802:CDS | 70.0% | |
| GTGGAGGTGGTGCAGCGGAG+TGG | + | 3511:691-710 | MS.gene068802:CDS | 70.0% | |
| GGTGGTGCAGCGGAGTGGGG+AGG | + | 3511:696-715 | MS.gene068802:CDS | 75.0% | |
| !! | CGGAGAGCACCGGAACCGGG+TGG | - | 3511:527-546 | MS.gene068802:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 3511 | gene | 121 | 885 | 121 | ID=MS.gene068802 |
| 3511 | mRNA | 121 | 885 | 121 | ID=MS.gene068802.t1;Parent=MS.gene068802 |
| 3511 | exon | 121 | 885 | 121 | ID=MS.gene068802.t1.exon1;Parent=MS.gene068802.t1 |
| 3511 | CDS | 121 | 885 | 121 | ID=cds.MS.gene068802.t1;Parent=MS.gene068802.t1 |
| Gene Sequence |
| Protein sequence |
