AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068927

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068927.t1 MTR_2g046030 95.396 391 15 1 1 391 1 388 0.0 761
MS.gene068927.t1 MTR_2g045980 94.629 391 18 1 1 391 1 388 0.0 751
MS.gene068927.t1 MTR_3g018740 69.133 392 116 2 1 391 1 388 0.0 523
MS.gene068927.t1 MTR_3g101010 68.031 391 119 2 1 389 1 387 1.00e-179 505
MS.gene068927.t1 MTR_8g027395 65.375 387 128 3 3 389 15 395 1.34e-176 497
MS.gene068927.t1 MTR_4g045707 64.858 387 130 3 4 389 5 386 3.31e-173 488
MS.gene068927.t1 MTR_3g048000 62.016 387 124 4 6 391 28 392 8.46e-164 465
MS.gene068927.t1 MTR_8g027420 62.016 387 130 3 3 389 4 373 4.59e-162 459
MS.gene068927.t1 MTR_8g461160 61.069 393 143 3 5 391 6 394 8.05e-159 452
MS.gene068927.t1 MTR_3g101030 60.000 400 134 6 10 389 34 427 2.67e-158 452
MS.gene068927.t1 MTR_4g088535 61.598 388 125 4 1 386 1 366 1.07e-156 446
MS.gene068927.t1 MTR_3g101130 59.439 392 144 5 4 389 5 387 6.28e-154 439
MS.gene068927.t1 MTR_4g088525 61.749 366 134 2 24 389 8 367 1.55e-153 438
MS.gene068927.t1 MTR_3g101180 58.458 402 150 6 1 389 1 398 1.20e-151 434
MS.gene068927.t1 MTR_7g050810 58.734 395 151 4 3 390 14 403 2.11e-151 434
MS.gene068927.t1 MTR_7g050750 57.975 395 154 4 3 390 6 395 7.90e-150 429
MS.gene068927.t1 MTR_7g451890 56.633 392 160 3 5 390 14 401 8.51e-148 424
MS.gene068927.t1 MTR_7g047780 57.468 395 156 4 3 390 12 401 4.37e-147 422
MS.gene068927.t1 MTR_3g101050 59.432 387 130 8 8 389 32 396 1.12e-141 409
MS.gene068927.t1 MTR_2g046410 74.182 275 26 2 117 391 120 349 1.12e-139 402
MS.gene068927.t1 MTR_3g015620 57.143 392 134 5 5 390 16 379 4.02e-137 397
MS.gene068927.t1 MTR_3g102920 56.510 384 121 8 12 389 65 408 3.75e-127 372
MS.gene068927.t1 MTR_3g101190 50.900 389 112 6 1 389 1 310 2.64e-118 346
MS.gene068927.t1 MTR_3g101200 50.510 392 159 7 3 389 29 390 1.63e-116 345
MS.gene068927.t1 MTR_3g018747 64.528 265 74 4 129 391 9 255 2.43e-110 324
MS.gene068927.t1 MTR_3g100995 61.390 259 93 3 133 390 11 263 1.08e-109 322
MS.gene068927.t1 MTR_3g100010 53.165 316 96 3 28 310 40 336 1.24e-102 307
MS.gene068927.t1 MTR_7g050830 50.784 319 120 4 5 301 16 319 3.25e-99 298
MS.gene068927.t1 MTR_3g111160 67.117 222 67 3 170 389 1 218 1.99e-92 277
MS.gene068927.t1 MTR_3g015760 59.023 266 99 4 128 390 22 280 2.01e-91 276
MS.gene068927.t1 MTR_3g100520 60.633 221 72 3 170 389 1 207 1.02e-86 262
MS.gene068927.t1 MTR_3g099970 62.500 200 54 3 129 328 20 198 1.66e-76 235
MS.gene068927.t1 MTR_3g114310 38.191 398 221 13 6 388 20 407 2.67e-73 234
MS.gene068927.t1 MTR_3g101110 52.263 243 80 5 129 365 68 280 3.33e-70 222
MS.gene068927.t1 MTR_3g111150 57.062 177 52 2 7 183 1 153 1.82e-60 193
MS.gene068927.t1 MTR_3g102870 62.821 156 54 1 192 347 1 152 4.49e-60 192
MS.gene068927.t1 MTR_3g101020 51.852 189 60 3 203 390 1 159 6.84e-57 184
MS.gene068927.t1 MTR_3g101150 58.385 161 57 3 235 389 1 157 3.59e-54 176
MS.gene068927.t1 MTR_3g100990 63.158 133 49 0 1 133 1 133 4.15e-47 168
MS.gene068927.t1 MTR_4g093550 57.471 87 37 0 2 88 3 89 2.51e-26 101
MS.gene068927.t1 MTR_6g046890 62.025 79 20 2 23 91 17 95 1.88e-19 84.3
MS.gene068927.t1 MTR_3g101140 55.224 67 27 1 326 389 48 114 1.83e-15 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068927.t1 AT1G47710 50.761 394 185 5 1 389 1 390 1.35e-135 393
MS.gene068927.t1 AT1G47710 50.761 394 185 5 1 389 28 417 9.81e-135 392
MS.gene068927.t1 AT3G45220 44.724 398 202 6 1 388 1 390 9.43e-112 332
MS.gene068927.t1 AT2G25240 45.844 397 191 9 1 388 1 382 2.41e-111 331
MS.gene068927.t1 AT2G26390 45.137 401 192 9 1 388 1 386 8.95e-111 330
MS.gene068927.t1 AT1G62170 39.031 392 205 6 5 388 66 431 1.07e-91 283
MS.gene068927.t1 AT2G14540 41.013 395 196 8 3 388 31 397 1.01e-88 274
MS.gene068927.t1 AT2G35580 39.646 396 206 9 1 388 1 371 6.00e-87 268
MS.gene068927.t1 AT1G64030 40.201 398 201 10 1 388 1 371 2.05e-86 267
MS.gene068927.t1 AT1G62170 36.000 425 205 7 5 388 66 464 1.00e-84 266
MS.gene068927.t1 AT1G64010 44.545 220 96 4 170 388 1 195 5.07e-59 191
MS.gene068927.t1 AT1G64010 43.415 205 90 4 185 388 2 181 4.68e-52 172
MS.gene068927.t1 AT1G63280 48.305 118 57 2 126 241 5 120 8.51e-30 111
MS.gene068927.t1 AT1G62160 38.916 203 92 5 192 388 42 218 1.40e-29 114
MS.gene068927.t1 AT1G51330 50.467 107 50 3 132 235 34 140 5.19e-22 93.2

Find 74 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTAATGCATTGTACTTTAT+AGG 0.187791 2.1:-45659337 MS.gene068927:CDS
GAGGAAGAAGTTCTTTAATA+AGG 0.302330 2.1:+45659395 None:intergenic
TTCGTGTAATAATTCATATT+TGG 0.303544 2.1:+45659072 None:intergenic
CGATAAGGCTGAGCACAATT+TGG 0.307568 2.1:+45660286 None:intergenic
CTCAGTCAAGGTTCCTTTCA+TGG 0.308297 2.1:-45659248 MS.gene068927:CDS
CCAAGATTCAATGTTTCATT+CGG 0.314871 2.1:-45658995 MS.gene068927:CDS
ACGGGAACAATCCTCTTTAT+CGG 0.322808 2.1:-45658710 MS.gene068927:CDS
TTTGGTTAGTGATTGATTCT+TGG 0.347320 2.1:+45660388 None:intergenic
GAAATATGTGAGAAACAAAA+AGG 0.360406 2.1:+45658876 None:intergenic
CAAGATTCAATGTTTCATTC+GGG 0.368803 2.1:-45658994 MS.gene068927:CDS
AAAATGGTGGATTCTCTTGC+GGG 0.387201 2.1:-45658905 MS.gene068927:CDS
ATCACGTACTATTTGTTTGA+AGG 0.408161 2.1:+45660074 None:intergenic
TGAAGGAAGGTTGAAGAGAA+AGG 0.413542 2.1:+45660091 None:intergenic
AACAACTTGTCGACTTCCTC+CGG 0.432314 2.1:-45660236 MS.gene068927:CDS
GATGATTAGTGGATTTGAAC+CGG 0.433973 2.1:+45660217 None:intergenic
AACCACTCCTAACTCCTTCA+AGG 0.438432 2.1:+45658955 None:intergenic
GAAGGAGTTAAGATGATTAG+TGG 0.445039 2.1:+45660206 None:intergenic
AGTGATACCTTGAAGGAGTT+AGG 0.458142 2.1:-45658962 MS.gene068927:CDS
GAAGGAAGGTTGAAGAGAAA+GGG 0.460189 2.1:+45660092 None:intergenic
GTGCTCAGCCTTATCGCTGC+TGG 0.467549 2.1:-45660279 MS.gene068927:CDS
CCGAATGAAACATTGAATCT+TGG 0.470101 2.1:+45658995 None:intergenic
TGAGCACTTGTCCGATAAAG+AGG 0.478855 2.1:+45658699 None:intergenic
TTCTCTAATTAGAAACAAGA+AGG 0.481714 2.1:+45658739 None:intergenic
ATCTTCTTGCTTACCATGAA+AGG 0.484270 2.1:+45659235 None:intergenic
CTTCAGAACCAGCAGCGATA+AGG 0.486899 2.1:+45660271 None:intergenic
TGTCGAGGGAAACCTTTGTT+TGG 0.488430 2.1:+45660370 None:intergenic
TGACTGAGCTTCCATTAAGA+AGG 0.502983 2.1:+45659263 None:intergenic
CGTACTATTTGTTTGAAGGA+AGG 0.504228 2.1:+45660078 None:intergenic
AAGGACGTTGCTCATGGTGG+TGG 0.504851 2.1:-45660156 MS.gene068927:CDS
TGAAACTAGTGATACCTTGA+AGG 0.507113 2.1:-45658969 MS.gene068927:CDS
ACGCTGGCTGATCTATCAAC+TGG 0.507175 2.1:+45659367 None:intergenic
TGGTTAACTTCAAAGTTTAG+TGG 0.507541 2.1:+45658764 None:intergenic
AGCAGTAGCTGCAGCAACTT+CGG 0.517350 2.1:+45658820 None:intergenic
TCTAATTAGAGAAGATTTGA+CGG 0.520817 2.1:-45658729 MS.gene068927:CDS
CTAATTAGAGAAGATTTGAC+GGG 0.523712 2.1:-45658728 MS.gene068927:CDS
CTTATCGCTGCTGGTTCTGA+AGG 0.525511 2.1:-45660270 MS.gene068927:CDS
TTGTTTCAACAAGAGATGTC+TGG 0.526031 2.1:+45660344 None:intergenic
TACTGCTGCAAGGTTGAGCA+AGG 0.529910 2.1:-45658804 MS.gene068927:CDS
TTCATCAGAGTAAATGAAGA+AGG 0.535238 2.1:-45658845 MS.gene068927:CDS
TAGGACGGAATGGAGATAAG+AGG 0.535652 2.1:+45660182 None:intergenic
CTACTCAAGGACGTTGCTCA+TGG 0.538812 2.1:-45660162 MS.gene068927:CDS
CGCTTAAATCACTCATCAAG+AGG 0.540255 2.1:+45658674 None:intergenic
AGCACAATTTGGAGTGATAG+TGG 0.543854 2.1:+45660297 None:intergenic
CATTGTACTTTATAGGAGCA+TGG 0.558936 2.1:-45659330 MS.gene068927:CDS
CGTCCTTGAGTAGGACGGAA+TGG 0.566952 2.1:+45660172 None:intergenic
GTCGACAAGTTGTTGTTGTG+TGG 0.569391 2.1:+45660245 None:intergenic
CAATTGAAACAAGTGAAAGT+AGG 0.570247 2.1:-45659031 MS.gene068927:CDS
CAGTTTATTGAAGCTTATGA+CGG 0.572175 2.1:-45659211 MS.gene068927:CDS
TCTCTAATTAGAAACAAGAA+GGG 0.572834 2.1:+45658740 None:intergenic
GTCAAACCTCCAGGAACGAA+TGG 0.572853 2.1:+45658929 None:intergenic
AGAGATGTCTGGTGATGTCG+AGG 0.585460 2.1:+45660355 None:intergenic
TCTCCATTCCGTCCTACTCA+AGG 0.586314 2.1:-45660175 MS.gene068927:CDS
TTGCTGCAGCTACTGCTGCA+AGG 0.592904 2.1:-45658814 MS.gene068927:CDS
TACCTTGAAGGAGTTAGGAG+TGG 0.601782 2.1:-45658957 MS.gene068927:CDS
CATCGACAAAAGAGAGGCGA+GGG 0.604715 2.1:+45660133 None:intergenic
TCGACAAGTTGTTGTTGTGT+GGG 0.607292 2.1:+45660246 None:intergenic
TCTTAATGGAAGCTCAGTCA+AGG 0.609827 2.1:-45659260 MS.gene068927:CDS
CTCAAGGACGTTGCTCATGG+TGG 0.626502 2.1:-45660159 MS.gene068927:CDS
TGATAGTTACAAAGCAACTC+CGG 0.626925 2.1:-45660055 MS.gene068927:CDS
CATGAGCAACGTCCTTGAGT+AGG 0.628321 2.1:+45660163 None:intergenic
GGAATGGAGATAAGAGGCGA+AGG 0.632838 2.1:+45660188 None:intergenic
ATCAATCACTAACCAAACAA+AGG 0.635395 2.1:-45660382 MS.gene068927:CDS
TAATTAGAAACAAGAAGGGG+TGG 0.635445 2.1:+45658744 None:intergenic
CTCTAATTAGAAACAAGAAG+GGG 0.642276 2.1:+45658741 None:intergenic
AGCAACGTCCTTGAGTAGGA+CGG 0.642446 2.1:+45660167 None:intergenic
TGCATTAGCAAAGATGACGC+TGG 0.651953 2.1:+45659351 None:intergenic
ATTAAAGAACTTCTTCCTCG+AGG 0.654402 2.1:-45659391 MS.gene068927:CDS
TTAACTTCAAAGTTTAGTGG+CGG 0.668795 2.1:+45658767 None:intergenic
CCATCGACAAAAGAGAGGCG+AGG 0.669374 2.1:+45660132 None:intergenic
GAGATGTCTGGTGATGTCGA+GGG 0.670600 2.1:+45660356 None:intergenic
GATCTATCAACTGGTCCTCG+AGG 0.670786 2.1:+45659376 None:intergenic
ACTGCTGCAAGGTTGAGCAA+GGG 0.682779 2.1:-45658803 MS.gene068927:CDS
GATTAGTGGATTTGAACCGG+AGG 0.688690 2.1:+45660220 None:intergenic
ATACACCATCGACAAAAGAG+AGG 0.689392 2.1:+45660127 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATAAGTTGATTTAATTAT+CGG - chr2.1:45659170-45659189 MS.gene068927:CDS 10.0%
!! AAGAGATATTTGAATAATAA+AGG + chr2.1:45659429-45659448 None:intergenic 15.0%
!! AAAAAAAGTGAATTTATGGT+CGG - chr2.1:45659644-45659663 MS.gene068927:intron 20.0%
!! AAAGAAATTCCAATTATGTA+AGG - chr2.1:45659402-45659421 MS.gene068927:CDS 20.0%
!! AAATATGTTGCTAAATTAGT+CGG + chr2.1:45659574-45659593 None:intergenic 20.0%
!! TGAAAGTTGTATTGTATATT+TGG - chr2.1:45659313-45659332 MS.gene068927:CDS 20.0%
!! TGACAAAAAAAGTGAATTTA+TGG - chr2.1:45659640-45659659 MS.gene068927:intron 20.0%
!! TTCGTGTAATAATTCATATT+TGG + chr2.1:45660009-45660028 None:intergenic 20.0%
!!! AAGTATTGCCAAAATATTTT+AGG + chr2.1:45659233-45659252 None:intergenic 20.0%
!!! AGTATTGCCAAAATATTTTA+GGG + chr2.1:45659232-45659251 None:intergenic 20.0%
! AAGTTGTATTGTATATTTGG+AGG - chr2.1:45659316-45659335 MS.gene068927:CDS 25.0%
! AATAAAGGTCCTTACATAAT+TGG + chr2.1:45659414-45659433 None:intergenic 25.0%
! AGTAAATCAAATCACATAGA+AGG + chr2.1:45659132-45659151 None:intergenic 25.0%
! ATAACTATGAACCTATCTTT+AGG + chr2.1:45659271-45659290 None:intergenic 25.0%
! GAAATATGTGAGAAACAAAA+AGG + chr2.1:45660205-45660224 None:intergenic 25.0%
! TAACTATGAACCTATCTTTA+GGG + chr2.1:45659270-45659289 None:intergenic 25.0%
! TCTAATTAGAGAAGATTTGA+CGG - chr2.1:45660349-45660368 MS.gene068927:CDS 25.0%
! TCTCTAATTAGAAACAAGAA+GGG + chr2.1:45660341-45660360 None:intergenic 25.0%
! TCTGATTATTGTATTTGATG+TGG - chr2.1:45659599-45659618 MS.gene068927:intron 25.0%
! TTCTCTAATTAGAAACAAGA+AGG + chr2.1:45660342-45660361 None:intergenic 25.0%
!! AAAATCACAGTTTTCTGTTT+TGG + chr2.1:45659793-45659812 None:intergenic 25.0%
!! AAGCAAACCCTAAAATATTT+TGG - chr2.1:45659222-45659241 MS.gene068927:CDS 25.0%
!!! AAAATATTTTAGGGTTTGCT+TGG + chr2.1:45659223-45659242 None:intergenic 25.0%
!!! GTATCTTGACCTTTTTTATA+AGG + chr2.1:45659912-45659931 None:intergenic 25.0%
!!! TGTATTTGATGTGGTTTTAA+AGG - chr2.1:45659608-45659627 MS.gene068927:intron 25.0%
!!! TTCACTTGATTTTTTAACCA+AGG - chr2.1:45659047-45659066 MS.gene068927:CDS 25.0%
AATCAATGAAATAACCTCGT+TGG - chr2.1:45659102-45659121 MS.gene068927:CDS 30.0%
ATCAATCACTAACCAAACAA+AGG - chr2.1:45658696-45658715 MS.gene068927:CDS 30.0%
ATCACGTACTATTTGTTTGA+AGG + chr2.1:45659007-45659026 None:intergenic 30.0%
CAAGATTCAATGTTTCATTC+GGG - chr2.1:45660084-45660103 MS.gene068927:CDS 30.0%
CAATTGAAACAAGTGAAAGT+AGG - chr2.1:45660047-45660066 MS.gene068927:CDS 30.0%
CAGTTTATTGAAGCTTATGA+CGG - chr2.1:45659867-45659886 MS.gene068927:intron 30.0%
CCAAGATTCAATGTTTCATT+CGG - chr2.1:45660083-45660102 MS.gene068927:CDS 30.0%
CTAATTAGAGAAGATTTGAC+GGG - chr2.1:45660350-45660369 MS.gene068927:CDS 30.0%
CTCTAATTAGAAACAAGAAG+GGG + chr2.1:45660340-45660359 None:intergenic 30.0%
GAGGAAGAAGTTCTTTAATA+AGG + chr2.1:45659686-45659705 None:intergenic 30.0%
GCTAATGCATTGTACTTTAT+AGG - chr2.1:45659741-45659760 MS.gene068927:intron 30.0%
GTTAAAAAATCAAGTGAAGC+CGG + chr2.1:45659045-45659064 None:intergenic 30.0%
TGGTTAACTTCAAAGTTTAG+TGG + chr2.1:45660317-45660336 None:intergenic 30.0%
TTAACTTCAAAGTTTAGTGG+CGG + chr2.1:45660314-45660333 None:intergenic 30.0%
TTCATCAGAGTAAATGAAGA+AGG - chr2.1:45660233-45660252 MS.gene068927:CDS 30.0%
! GTATATTTGGAGGTATGAAT+TGG - chr2.1:45659326-45659345 MS.gene068927:CDS 30.0%
! TGTGATTTTCACCTTCTTAA+TGG - chr2.1:45659804-45659823 MS.gene068927:intron 30.0%
! TTTGGTTAGTGATTGATTCT+TGG + chr2.1:45658693-45658712 None:intergenic 30.0%
!! TGCTCTAAAATCATTGCTAA+AGG + chr2.1:45659488-45659507 None:intergenic 30.0%
!! TTTGATAGTGTAGCTTACTT+CGG - chr2.1:45659356-45659375 MS.gene068927:CDS 30.0%
!!! CTTGACCTTTTTTATAAGGA+AGG + chr2.1:45659908-45659927 None:intergenic 30.0%
ATCTTCTTGCTTACCATGAA+AGG + chr2.1:45659846-45659865 None:intergenic 35.0%
ATTAAAGAACTTCTTCCTCG+AGG - chr2.1:45659687-45659706 MS.gene068927:intron 35.0%
CATTGTACTTTATAGGAGCA+TGG - chr2.1:45659748-45659767 MS.gene068927:intron 35.0%
CCGAATGAAACATTGAATCT+TGG + chr2.1:45660086-45660105 None:intergenic 35.0%
CGTACTATTTGTTTGAAGGA+AGG + chr2.1:45659003-45659022 None:intergenic 35.0%
CTTCGCCTTCCTTATAAAAA+AGG - chr2.1:45659900-45659919 MS.gene068927:intron 35.0%
GAAGGAGTTAAGATGATTAG+TGG + chr2.1:45658875-45658894 None:intergenic 35.0%
TAATTAGAAACAAGAAGGGG+TGG + chr2.1:45660337-45660356 None:intergenic 35.0%
TGAAACTAGTGATACCTTGA+AGG - chr2.1:45660109-45660128 MS.gene068927:CDS 35.0%
TGATAGTTACAAAGCAACTC+CGG - chr2.1:45659023-45659042 MS.gene068927:CDS 35.0%
TTGTTTCAACAAGAGATGTC+TGG + chr2.1:45658737-45658756 None:intergenic 35.0%
! TTTTTGCCAAACGCAAAAGA+TGG - chr2.1:45659954-45659973 MS.gene068927:intron 35.0%
!! AAAAATGGTGGATTCTCTTG+CGG - chr2.1:45660172-45660191 MS.gene068927:CDS 35.0%
!! GATGATTAGTGGATTTGAAC+CGG + chr2.1:45658864-45658883 None:intergenic 35.0%
!!! CACAGTTTTCTGTTTTGGAA+AGG + chr2.1:45659788-45659807 None:intergenic 35.0%
AAACTGAGATATGCACACCT+TGG + chr2.1:45659067-45659086 None:intergenic 40.0%
ACGGGAACAATCCTCTTTAT+CGG - chr2.1:45660368-45660387 MS.gene068927:CDS 40.0%
AGACCAAGTACCCTAAAGAT+AGG - chr2.1:45659257-45659276 MS.gene068927:CDS 40.0%
AGTGATACCTTGAAGGAGTT+AGG - chr2.1:45660116-45660135 MS.gene068927:CDS 40.0%
ATACACCATCGACAAAAGAG+AGG + chr2.1:45658954-45658973 None:intergenic 40.0%
GAAGGAAGGTTGAAGAGAAA+GGG + chr2.1:45658989-45659008 None:intergenic 40.0%
TCCTGGAGGTTTGACAAAAA+TGG - chr2.1:45660157-45660176 MS.gene068927:CDS 40.0%
TCTTAATGGAAGCTCAGTCA+AGG - chr2.1:45659818-45659837 MS.gene068927:intron 40.0%
TGAAGGAAGGTTGAAGAGAA+AGG + chr2.1:45658990-45659009 None:intergenic 40.0%
TGACTGAGCTTCCATTAAGA+AGG + chr2.1:45659818-45659837 None:intergenic 40.0%
TGGAGGTTTGACAAAAATGG+TGG - chr2.1:45660160-45660179 MS.gene068927:CDS 40.0%
TGGTCGGAAAAAGAGACAAT+TGG - chr2.1:45659660-45659679 MS.gene068927:intron 40.0%
! AGCACAATTTGGAGTGATAG+TGG + chr2.1:45658784-45658803 None:intergenic 40.0%
! CTCTTTTGTCGATGGTGTAT+GGG - chr2.1:45658954-45658973 MS.gene068927:CDS 40.0%
! TCGACAAGTTGTTGTTGTGT+GGG + chr2.1:45658835-45658854 None:intergenic 40.0%
! TCTCTTTTGTCGATGGTGTA+TGG - chr2.1:45658953-45658972 MS.gene068927:CDS 40.0%
!! AAAATGGTGGATTCTCTTGC+GGG - chr2.1:45660173-45660192 MS.gene068927:CDS 40.0%
!! ACCATTTTTGTCAAACCTCC+AGG + chr2.1:45660161-45660180 None:intergenic 40.0%
!! GAACCTATCTTTAGGGTACT+TGG + chr2.1:45659263-45659282 None:intergenic 40.0%
AACCACTCCTAACTCCTTCA+AGG + chr2.1:45660126-45660145 None:intergenic 45.0%
ATGCTCGAAACTGTTAGAGG+AGG + chr2.1:45659551-45659570 None:intergenic 45.0%
CGATAAGGCTGAGCACAATT+TGG + chr2.1:45658795-45658814 None:intergenic 45.0%
CTCAGTCAAGGTTCCTTTCA+TGG - chr2.1:45659830-45659849 MS.gene068927:intron 45.0%
TACCTTGAAGGAGTTAGGAG+TGG - chr2.1:45660121-45660140 MS.gene068927:CDS 45.0%
TAGGACGGAATGGAGATAAG+AGG + chr2.1:45658899-45658918 None:intergenic 45.0%
TGAGCACTTGTCCGATAAAG+AGG + chr2.1:45660382-45660401 None:intergenic 45.0%
TGCATTAGCAAAGATGACGC+TGG + chr2.1:45659730-45659749 None:intergenic 45.0%
TGTCGAGGGAAACCTTTGTT+TGG + chr2.1:45658711-45658730 None:intergenic 45.0%
! GACAGTCCATCTTTTGCGTT+TGG + chr2.1:45659963-45659982 None:intergenic 45.0%
! GTCGACAAGTTGTTGTTGTG+TGG + chr2.1:45658836-45658855 None:intergenic 45.0%
! GTTTTACCATTCGTTCCTGG+AGG - chr2.1:45660143-45660162 MS.gene068927:CDS 45.0%
! TTAGGGTTTGCTTGGAACAG+TGG + chr2.1:45659215-45659234 None:intergenic 45.0%
!! AACAACTTGTCGACTTCCTC+CGG - chr2.1:45658842-45658861 MS.gene068927:CDS 45.0%
!! GATTAGTGGATTTGAACCGG+AGG + chr2.1:45658861-45658880 None:intergenic 45.0%
!! GTGGTTTTACCATTCGTTCC+TGG - chr2.1:45660140-45660159 MS.gene068927:CDS 45.0%
ACTGCTGCAAGGTTGAGCAA+GGG - chr2.1:45660275-45660294 MS.gene068927:CDS 50.0%
AGCAACGTCCTTGAGTAGGA+CGG + chr2.1:45658914-45658933 None:intergenic 50.0%
AGCAGTAGCTGCAGCAACTT+CGG + chr2.1:45660261-45660280 None:intergenic 50.0%
CATCGACAAAAGAGAGGCGA+GGG + chr2.1:45658948-45658967 None:intergenic 50.0%
CATGAGCAACGTCCTTGAGT+AGG + chr2.1:45658918-45658937 None:intergenic 50.0%
CGGATGCTCGAAACTGTTAG+AGG + chr2.1:45659554-45659573 None:intergenic 50.0%
CTACTCAAGGACGTTGCTCA+TGG - chr2.1:45658916-45658935 MS.gene068927:CDS 50.0%
CTTCAGAACCAGCAGCGATA+AGG + chr2.1:45658810-45658829 None:intergenic 50.0%
GATCTATCAACTGGTCCTCG+AGG + chr2.1:45659705-45659724 None:intergenic 50.0%
GGAATGGAGATAAGAGGCGA+AGG + chr2.1:45658893-45658912 None:intergenic 50.0%
GTCAAACCTCCAGGAACGAA+TGG + chr2.1:45660152-45660171 None:intergenic 50.0%
TACTGCTGCAAGGTTGAGCA+AGG - chr2.1:45660274-45660293 MS.gene068927:CDS 50.0%
TCTCCATTCCGTCCTACTCA+AGG - chr2.1:45658903-45658922 MS.gene068927:CDS 50.0%
! ACATAGAAGGCACACCAACG+AGG + chr2.1:45659119-45659138 None:intergenic 50.0%
! ACGCTGGCTGATCTATCAAC+TGG + chr2.1:45659714-45659733 None:intergenic 50.0%
! AGAGATGTCTGGTGATGTCG+AGG + chr2.1:45658726-45658745 None:intergenic 50.0%
! GAGATGTCTGGTGATGTCGA+GGG + chr2.1:45658725-45658744 None:intergenic 50.0%
!! CTTATCGCTGCTGGTTCTGA+AGG - chr2.1:45658808-45658827 MS.gene068927:CDS 50.0%
AAGGACGTTGCTCATGGTGG+TGG - chr2.1:45658922-45658941 MS.gene068927:CDS 55.0%
CCATCGACAAAAGAGAGGCG+AGG + chr2.1:45658949-45658968 None:intergenic 55.0%
CGTCCTTGAGTAGGACGGAA+TGG + chr2.1:45658909-45658928 None:intergenic 55.0%
CTCAAGGACGTTGCTCATGG+TGG - chr2.1:45658919-45658938 MS.gene068927:CDS 55.0%
TTGCTGCAGCTACTGCTGCA+AGG - chr2.1:45660264-45660283 MS.gene068927:CDS 55.0%
! CCTCGCCTCTCTTTTGTCGA+TGG - chr2.1:45658946-45658965 MS.gene068927:CDS 55.0%
! GTGCTCAGCCTTATCGCTGC+TGG - chr2.1:45658799-45658818 MS.gene068927:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 45658682 45660418 45658682 ID=MS.gene068927
chr2.1 mRNA 45658682 45660418 45658682 ID=MS.gene068927.t1;Parent=MS.gene068927
chr2.1 exon 45660032 45660418 45660032 ID=MS.gene068927.t1.exon1;Parent=MS.gene068927.t1
chr2.1 CDS 45660032 45660418 45660032 ID=cds.MS.gene068927.t1;Parent=MS.gene068927.t1
chr2.1 exon 45658682 45659470 45658682 ID=MS.gene068927.t1.exon2;Parent=MS.gene068927.t1
chr2.1 CDS 45658682 45659470 45658682 ID=cds.MS.gene068927.t1;Parent=MS.gene068927.t1
Gene Sequence

>MS.gene068927

ATGGCTCTCCAAGAATCAATCACTAACCAAACAAAGGTTTCCCTCGACATCACCAGACATCTCTTGTTGAAACAATCAGACAAAAACGTCGTGTTTTCACCACTATCACTCCAAATTGTGCTCAGCCTTATCGCTGCTGGTTCTGAAGGTCCCACACAACAACAACTTGTCGACTTCCTCCGGTTCAAATCCACTAATCATCTTAACTCCTTCGCCTCTTATCTCCATTCCGTCCTACTCAAGGACGTTGCTCATGGTGGTGGCCCTCGCCTCTCTTTTGTCGATGGTGTATGGGTTGAACAAACCCTTTCTCTTCAACCTTCCTTCAAACAAATAGTACGTGATAGTTACAAAGCAACTCCGGCTTCACTTGATTTTTTAACCAAGGTGTGCATATCTCAGTTTTTTTTTCTTTCTGTTAATCAATGAAATAACCTCGTTGGTGTGCCTTCTATGTGATTTGATTTACTTAGTTGCATGATGTATTTTAATAAGTTGATTTAATTATCGGAAGAAAAAAAAAAGCAATACCACTGTTCCAAGCAAACCCTAAAATATTTTGGCAATACTTCTAAAGACCAAGTACCCTAAAGATAGGTTCATAGTTATAATGTTTTGCATTTATTTGAATTGAAAGTTGTATTGTATATTTGGAGGTATGAATTGGTTTATATTTTGATAGTGTAGCTTACTTCGGTAAATACAAATTCTAAACTAATCAAAGAAATTCCAATTATGTAAGGACCTTTATTATTCAAATATCTCTTCAAATTTTATAATACTAAAGAGAAATAAACTTTAATCCTTTAGCAATGATTTTAGAGCATCAATCAACTTCTATGAAAATAATTGACGAATGCAACTTTCCTCCTCTAACAGTTTCGAGCATCCGACTAATTTAGCAACATATTTGCATATCTGATTATTGTATTTGATGTGGTTTTAAAGGTTGTTGAAGTGACAAAAAAAGTGAATTTATGGTCGGAAAAAGAGACAATTGGCCTTATTAAAGAACTTCTTCCTCGAGGACCAGTTGATAGATCAGCCAGCGTCATCTTTGCTAATGCATTGTACTTTATAGGAGCATGGAATGAGAAATTCGACCTTTCCAAAACAGAAAACTGTGATTTTCACCTTCTTAATGGAAGCTCAGTCAAGGTTCCTTTCATGGTAAGCAAGAAGATGCAGTTTATTGAAGCTTATGACGGTTCTAAAGTTCTTCGCCTTCCTTATAAAAAAGGTCAAGATACGCGACAATTCTCTATGTATATTTTTTTGCCAAACGCAAAAGATGGACTGTCAGCTTTAATCGAGAAGATGACTTCCAAATATGAATTATTACACGAAAAGCTTAGTCTTTATGATCAATTGAAACAAGTGAAAGTAGGTGATTTTAAAATTCCAAGATTCAATGTTTCATTCGGGCTTGAAACTAGTGATACCTTGAAGGAGTTAGGAGTGGTTTTACCATTCGTTCCTGGAGGTTTGACAAAAATGGTGGATTCTCTTGCGGGTCGAAGCCTTTTTGTTTCTCACATATTTCACAAGTCTTTCATCAGAGTAAATGAAGAAGGTACCGAAGTTGCTGCAGCTACTGCTGCAAGGTTGAGCAAGGGTTGTTCATTTTCTCCGCCACTAAACTTTGAAGTTAACCACCCCTTCTTGTTTCTAATTAGAGAAGATTTGACGGGAACAATCCTCTTTATCGGACAAGTGCTCAATCCTCTTGATGAGTGA
Protein sequence

>MS.gene068927.t1

MALQESITNQTKVSLDITRHLLLKQSDKNVVFSPLSLQIVLSLIAAGSEGPTQQQLVDFLRFKSTNHLNSFASYLHSVLLKDVAHGGGPRLSFVDGVWVEQTLSLQPSFKQIVRDSYKATPASLDFLTKVVEVTKKVNLWSEKETIGLIKELLPRGPVDRSASVIFANALYFIGAWNEKFDLSKTENCDFHLLNGSSVKVPFMVSKKMQFIEAYDGSKVLRLPYKKGQDTRQFSMYIFLPNAKDGLSALIEKMTSKYELLHEKLSLYDQLKQVKVGDFKIPRFNVSFGLETSDTLKELGVVLPFVPGGLTKMVDSLAGRSLFVSHIFHKSFIRVNEEGTEVAAATAARLSKGCSFSPPLNFEVNHPFLFLIREDLTGTILFIGQVLNPLDE