Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069301.t1 | XP_024633936.1 | 97.8 | 91 | 2 | 0 | 1 | 91 | 1 | 91 | 9.60E-39 | 169.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069301.t1 | G7IVT7 | 97.8 | 91 | 2 | 0 | 1 | 91 | 50 | 140 | 6.9e-39 | 169.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053147 | MS.gene069301 | -0.80224 | 6.16E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069301.t1 | MTR_3g013450 | 97.802 | 91 | 2 | 0 | 1 | 91 | 50 | 140 | 1.65e-60 | 181 |
| MS.gene069301.t1 | MTR_3g013450 | 97.802 | 91 | 2 | 0 | 1 | 91 | 55 | 145 | 2.54e-60 | 181 |
| MS.gene069301.t1 | MTR_1g052680 | 75.000 | 92 | 12 | 3 | 1 | 91 | 1 | 82 | 3.17e-41 | 130 |
| MS.gene069301.t1 | MTR_5g020180 | 39.394 | 66 | 38 | 2 | 1 | 64 | 14 | 79 | 6.69e-13 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069301.t1 | AT4G39235 | 64.835 | 91 | 27 | 1 | 1 | 91 | 1 | 86 | 6.56e-36 | 117 |
| MS.gene069301.t1 | AT4G39235 | 64.835 | 91 | 27 | 1 | 1 | 91 | 1 | 86 | 6.56e-36 | 117 |
| MS.gene069301.t1 | AT3G05570 | 63.043 | 92 | 31 | 2 | 1 | 91 | 1 | 90 | 1.03e-34 | 114 |
| MS.gene069301.t1 | AT2G21640 | 50.538 | 93 | 42 | 3 | 1 | 89 | 10 | 102 | 4.75e-23 | 85.1 |
Find 14 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAGTTTATTCATGCTTCTA+TGG | 0.253989 | 3.3:-8411639 | MS.gene069301:CDS |
| TTGGATCTATTTCAGATGTT+TGG | 0.301015 | 3.3:-8411500 | MS.gene069301:intron |
| GCATGAAGAAGCCTCATTCT+TGG | 0.389977 | 3.3:-8411684 | MS.gene069301:CDS |
| TTCAACTTCTTTCGTAGCCT+TGG | 0.399403 | 3.3:+8411448 | None:intergenic |
| CTTCTTCTTTCGACAAGAAG+TGG | 0.455215 | 3.3:+8411706 | None:intergenic |
| GTTTGGTTTGTCCAAGGTAG+TGG | 0.458713 | 3.3:-8411483 | MS.gene069301:CDS |
| CTTTCAGAAAACAATTAAGA+AGG | 0.462199 | 3.3:-8411600 | MS.gene069301:intron |
| CTCATTCTTGGAGGATGTGA+AGG | 0.535627 | 3.3:-8411672 | MS.gene069301:CDS |
| CTTCACATCCTCCAAGAATG+AGG | 0.544616 | 3.3:+8411673 | None:intergenic |
| GCAACAGCAGCTGATGAAGT+TGG | 0.545155 | 3.3:+8411728 | None:intergenic |
| GTTCGCTTCAGCCACTACCT+TGG | 0.562123 | 3.3:+8411472 | None:intergenic |
| TGAAGAAGCCTCATTCTTGG+AGG | 0.565258 | 3.3:-8411681 | MS.gene069301:CDS |
| TCAGATGTTTGGTTTGTCCA+AGG | 0.567621 | 3.3:-8411489 | MS.gene069301:intron |
| AGTGGCTGAAGCGAACTCCA+AGG | 0.641621 | 3.3:-8411465 | MS.gene069301:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTTTCAGAAAACAATTAAGA+AGG | - | chr3.3:8411570-8411589 | MS.gene069301:intron | 25.0% |
| ! | TAAATTTAACAGGATAGCTT+TGG | + | chr3.3:8411609-8411628 | None:intergenic | 25.0% |
| GTGGTGCAAATAAATTTAAC+AGG | + | chr3.3:8411619-8411638 | None:intergenic | 30.0% | |
| TTGGATCTATTTCAGATGTT+TGG | - | chr3.3:8411670-8411689 | MS.gene069301:CDS | 30.0% | |
| !! | CTTAATTGTTTTCTGAAAGC+AGG | + | chr3.3:8411569-8411588 | None:intergenic | 30.0% |
| CGAGTTTATTCATGCTTCTA+TGG | - | chr3.3:8411531-8411550 | MS.gene069301:intron | 35.0% | |
| TCAGCAAATGACAAAGAATG+TGG | + | chr3.3:8411638-8411657 | None:intergenic | 35.0% | |
| ! | TGTCATTTGCTGATATAGTG+TGG | - | chr3.3:8411645-8411664 | MS.gene069301:CDS | 35.0% |
| ! | TTGCTGATATAGTGTGGTTT+TGG | - | chr3.3:8411651-8411670 | MS.gene069301:CDS | 35.0% |
| !!! | TCTTTTAAAACGGTCTGAAG+AGG | + | chr3.3:8411757-8411776 | None:intergenic | 35.0% |
| CTTCTTCTTTCGACAAGAAG+TGG | + | chr3.3:8411467-8411486 | None:intergenic | 40.0% | |
| TTCAACTTCTTTCGTAGCCT+TGG | + | chr3.3:8411725-8411744 | None:intergenic | 40.0% | |
| !! | TCAGATGTTTGGTTTGTCCA+AGG | - | chr3.3:8411681-8411700 | MS.gene069301:CDS | 40.0% |
| !!! | ATGAAGTTGGCTTTTCAGCT+TGG | + | chr3.3:8411432-8411451 | None:intergenic | 40.0% |
| CTCATTCTTGGAGGATGTGA+AGG | - | chr3.3:8411498-8411517 | MS.gene069301:intron | 45.0% | |
| CTTCACATCCTCCAAGAATG+AGG | + | chr3.3:8411500-8411519 | None:intergenic | 45.0% | |
| GCATGAAGAAGCCTCATTCT+TGG | - | chr3.3:8411486-8411505 | MS.gene069301:CDS | 45.0% | |
| TGAAGAAGCCTCATTCTTGG+AGG | - | chr3.3:8411489-8411508 | MS.gene069301:intron | 45.0% | |
| !! | GTTTGGTTTGTCCAAGGTAG+TGG | - | chr3.3:8411687-8411706 | MS.gene069301:CDS | 45.0% |
| GCAACAGCAGCTGATGAAGT+TGG | + | chr3.3:8411445-8411464 | None:intergenic | 50.0% | |
| AGTGGCTGAAGCGAACTCCA+AGG | - | chr3.3:8411705-8411724 | MS.gene069301:CDS | 55.0% | |
| GTTCGCTTCAGCCACTACCT+TGG | + | chr3.3:8411701-8411720 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 8411412 | 8411780 | 8411412 | ID=MS.gene069301 |
| chr3.3 | mRNA | 8411412 | 8411780 | 8411412 | ID=MS.gene069301.t1;Parent=MS.gene069301 |
| chr3.3 | exon | 8411601 | 8411780 | 8411601 | ID=MS.gene069301.t1.exon1;Parent=MS.gene069301.t1 |
| chr3.3 | CDS | 8411601 | 8411780 | 8411601 | ID=cds.MS.gene069301.t1;Parent=MS.gene069301.t1 |
| chr3.3 | exon | 8411412 | 8411507 | 8411412 | ID=MS.gene069301.t1.exon2;Parent=MS.gene069301.t1 |
| chr3.3 | CDS | 8411412 | 8411507 | 8411412 | ID=cds.MS.gene069301.t1;Parent=MS.gene069301.t1 |
| Gene Sequence |
| Protein sequence |
