AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069416

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069416.t1 MTR_7g013790 98.611 144 2 0 19 162 276 419 1.82e-104 307
MS.gene069416.t1 MTR_1g095560 55.000 140 54 2 19 158 277 407 6.84e-49 164
MS.gene069416.t1 MTR_1g095560 55.000 140 54 2 19 158 277 407 7.69e-49 164
MS.gene069416.t1 MTR_1g095540 50.714 140 60 2 19 158 277 407 1.44e-43 150
MS.gene069416.t1 MTR_2g062990 46.099 141 65 2 19 158 283 413 8.79e-41 143
MS.gene069416.t1 MTR_2g062980 46.429 140 66 1 19 158 283 413 4.79e-40 140
MS.gene069416.t1 MTR_2g063000 45.714 140 67 1 19 158 253 383 1.95e-39 139
MS.gene069416.t1 MTR_2g063000 45.714 140 67 1 19 158 284 414 2.88e-39 139
MS.gene069416.t1 MTR_3g034290 42.069 145 69 2 18 162 315 444 1.27e-37 135
MS.gene069416.t1 MTR_4g075150 45.890 146 71 3 18 162 278 416 2.17e-36 131
MS.gene069416.t1 MTR_4g075150 45.890 146 73 3 18 162 278 418 3.69e-36 130
MS.gene069416.t1 MTR_2g012860 40.411 146 87 0 19 164 285 430 4.33e-36 130
MS.gene069416.t1 MTR_4g089155 41.096 146 86 0 19 164 297 442 9.13e-36 129
MS.gene069416.t1 MTR_2g012850 41.096 146 86 0 19 164 277 422 7.98e-35 127
MS.gene069416.t1 MTR_2g081620 45.000 140 68 2 19 158 284 414 1.04e-34 126
MS.gene069416.t1 MTR_0003s0560 41.549 142 68 2 16 157 310 436 2.64e-33 123
MS.gene069416.t1 MTR_3g079570 40.972 144 67 3 19 162 320 445 2.36e-31 117
MS.gene069416.t1 MTR_3g079560 40.972 144 67 3 19 162 320 445 6.63e-31 116
MS.gene069416.t1 MTR_3g079600 40.972 144 67 3 19 162 321 446 8.75e-31 116
MS.gene069416.t1 MTR_1g021010 40.972 144 64 4 19 162 319 441 2.42e-30 115
MS.gene069416.t1 MTR_1g020090 39.583 144 64 3 19 162 332 452 5.59e-30 114
MS.gene069416.t1 MTR_1g021020 40.278 144 65 4 19 162 320 442 1.72e-29 112
MS.gene069416.t1 MTR_4g122950 38.854 157 78 3 19 162 281 432 3.67e-29 111
MS.gene069416.t1 MTR_3g079590 41.497 147 63 4 19 162 319 445 6.48e-29 111
MS.gene069416.t1 MTR_3g079620 36.601 153 70 4 19 162 319 453 1.07e-26 105
MS.gene069416.t1 MTR_4g122960 34.524 168 94 3 6 162 268 430 1.87e-26 104
MS.gene069416.t1 MTR_5g072570 39.496 119 68 1 48 162 297 415 1.92e-24 98.6
MS.gene069416.t1 MTR_3g079630 36.129 155 68 5 19 162 319 453 3.01e-23 95.5
MS.gene069416.t1 MTR_3g034290 39.450 109 51 2 18 126 315 408 2.22e-21 89.7
MS.gene069416.t1 MTR_3g090150 43.678 87 49 0 60 146 311 397 2.10e-20 87.4
MS.gene069416.t1 MTR_3g090130 36.975 119 71 1 48 162 292 410 4.00e-20 86.7
MS.gene069416.t1 MTR_8g099930 44.860 107 54 3 60 162 311 416 4.04e-20 86.7
MS.gene069416.t1 MTR_3g498755 41.121 107 58 2 60 162 294 399 1.58e-18 82.0
MS.gene069416.t1 MTR_7g021130 37.383 107 62 3 60 162 306 411 4.27e-18 80.9
MS.gene069416.t1 MTR_1g102130 41.379 87 50 1 60 146 339 424 5.87e-17 77.4
MS.gene069416.t1 MTR_2g449850 39.024 123 65 5 44 162 300 416 7.84e-17 77.0
MS.gene069416.t1 MTR_3g434850 38.318 107 61 3 60 162 303 408 1.07e-15 73.9
MS.gene069416.t1 MTR_1g040370 33.645 107 64 2 60 162 266 369 7.45e-15 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069416.t1 AT3G02110 75.168 149 32 1 19 162 278 426 1.90e-80 246
MS.gene069416.t1 AT4G30610 56.250 144 62 1 19 162 275 417 3.06e-54 178
MS.gene069416.t1 AT2G24010 52.778 144 59 1 19 162 272 406 1.50e-50 168
MS.gene069416.t1 AT2G24010 52.778 144 59 1 19 162 283 417 2.11e-50 168
MS.gene069416.t1 AT2G24000 54.422 147 62 3 19 162 209 353 2.15e-50 166
MS.gene069416.t1 AT2G24010 52.778 144 59 1 19 162 287 421 2.37e-50 168
MS.gene069416.t1 AT2G24000 54.422 147 62 3 19 162 272 416 4.24e-50 167
MS.gene069416.t1 AT3G07990 49.645 141 60 2 19 158 278 408 2.17e-43 149
MS.gene069416.t1 AT2G35780 46.763 139 67 1 19 157 270 401 2.97e-41 144
MS.gene069416.t1 AT3G63470 44.444 144 67 2 19 162 321 451 8.28e-40 140
MS.gene069416.t1 AT4G30810 45.139 144 73 2 19 162 280 417 2.91e-37 134
MS.gene069416.t1 AT5G23210 43.976 166 78 5 14 165 193 357 4.48e-37 131
MS.gene069416.t1 AT5G23210 44.172 163 76 5 14 162 193 354 2.19e-36 130
MS.gene069416.t1 AT5G23210 44.172 163 76 5 14 162 194 355 2.40e-36 130
MS.gene069416.t1 AT5G23210 43.976 166 78 5 14 165 289 453 2.67e-36 130
MS.gene069416.t1 AT5G23210 44.172 163 76 5 14 162 289 450 8.77e-36 130
MS.gene069416.t1 AT5G08260 45.946 148 75 2 19 162 284 430 1.76e-35 129
MS.gene069416.t1 AT1G61130 46.667 150 68 6 19 164 276 417 1.80e-35 128
MS.gene069416.t1 AT1G11080 45.455 154 72 4 19 164 297 446 5.36e-35 127
MS.gene069416.t1 AT1G11080 45.455 154 72 4 19 164 297 446 6.52e-35 127
MS.gene069416.t1 AT4G15100 44.156 154 74 4 19 164 289 438 7.43e-35 127
MS.gene069416.t1 AT2G24010 48.148 108 47 1 19 126 258 356 1.10e-31 117
MS.gene069416.t1 AT2G05850 39.474 152 78 2 19 168 305 444 1.90e-30 115
MS.gene069416.t1 AT3G52010 38.816 152 79 2 19 168 305 444 2.97e-30 114
MS.gene069416.t1 AT3G52000 37.576 165 89 2 6 168 287 439 9.56e-29 110
MS.gene069416.t1 AT3G17180 37.267 161 95 4 7 162 270 429 8.49e-28 107
MS.gene069416.t1 AT3G17180 37.267 161 95 4 7 162 270 429 1.46e-27 107
MS.gene069416.t1 AT2G35770 37.857 140 74 3 19 158 280 406 1.10e-25 102
MS.gene069416.t1 AT3G52020 39.474 114 64 3 59 169 348 459 3.25e-25 100
MS.gene069416.t1 AT1G43780 37.500 120 71 1 47 162 305 424 3.38e-24 98.2
MS.gene069416.t1 AT5G42240 42.017 119 65 2 48 162 300 418 1.24e-23 96.7
MS.gene069416.t1 AT5G42230 38.655 119 69 1 48 162 296 414 1.08e-21 90.9
MS.gene069416.t1 AT5G42230 38.655 119 69 1 48 162 296 414 1.08e-21 90.9
MS.gene069416.t1 AT2G12480 41.121 107 59 1 60 162 305 411 1.41e-20 87.8
MS.gene069416.t1 AT2G33530 40.187 107 59 2 60 162 311 416 4.75e-18 80.9
MS.gene069416.t1 AT1G28110 38.318 107 61 2 60 162 307 412 1.27e-17 79.3
MS.gene069416.t1 AT1G28110 38.318 107 61 2 60 162 307 412 1.27e-17 79.3
MS.gene069416.t1 AT2G12480 42.857 91 48 1 76 162 297 387 3.34e-17 78.2
MS.gene069416.t1 AT2G12480 42.045 88 47 1 79 162 298 385 6.56e-16 74.7
MS.gene069416.t1 AT2G12480 42.045 88 47 1 79 162 298 385 6.83e-16 74.3

Find 37 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATATCTTGTTGCTGTAAC+TGG 0.291457 7.2:-83601053 None:intergenic
TATGCCATGGATCAAGAATT+TGG 0.305898 7.2:+83599516 MS.gene069416:CDS
CTTATAAGTGGACTGCTTGC+AGG 0.308616 7.2:+83600357 MS.gene069416:CDS
TAGCTCATGGAGTAAGAGTT+TGG 0.323540 7.2:+83600560 MS.gene069416:CDS
ATATAAAGATTCACATTCAT+TGG 0.330653 7.2:-83599490 None:intergenic
CTGCAAGCAGTCCACTTATA+AGG 0.332916 7.2:-83600356 None:intergenic
CCGGTACCCGCCACACTATT+CGG 0.384832 7.2:+83599602 MS.gene069416:CDS
ATGCAGAAGTTTACTACAAT+AGG 0.398481 7.2:+83600291 MS.gene069416:CDS
CTAAAATACCATGGTATCCT+TGG 0.425051 7.2:+83601099 MS.gene069416:CDS
AATTCCGACGGCAGCTCGTC+CGG 0.461016 7.2:+83599583 MS.gene069416:CDS
GCACTTCATGCTAACACAAC+AGG 0.472619 7.2:+83600329 MS.gene069416:CDS
CCGAATAGTGTGGCGGGTAC+CGG 0.475453 7.2:-83599602 None:intergenic
GCTATCAATTCTCTATAAAG+TGG 0.486119 7.2:-83600541 None:intergenic
TATAGAGAATTGATAGCTCA+TGG 0.488532 7.2:+83600547 MS.gene069416:CDS
TGCCCTTGCACAGCTTAAGT+TGG 0.489165 7.2:+83601073 MS.gene069416:CDS
GTTACCAAATTCTTGATCCA+TGG 0.508473 7.2:-83599520 None:intergenic
CAACAGGCATTCCTTATAAG+TGG 0.512094 7.2:+83600345 MS.gene069416:CDS
CTGCCGTCGGAATTGTTACA+TGG 0.537209 7.2:-83599574 None:intergenic
ATCTTTATATAGCTATGCCA+TGG 0.571081 7.2:+83599503 MS.gene069416:CDS
GGGCAACCGAATAGTGTGGC+GGG 0.573112 7.2:-83599608 None:intergenic
GTATACCACATGAGGAAGGT+GGG 0.574762 7.2:-83599629 None:intergenic
GTTGCCCCACCTTCCTCATG+TGG 0.589592 7.2:+83599624 MS.gene069416:CDS
TAGCCAACTTAAGCTGTGCA+AGG 0.592162 7.2:-83601076 None:intergenic
AGCCAACTTAAGCTGTGCAA+GGG 0.595378 7.2:-83601075 None:intergenic
AGTATACCACATGAGGAAGG+TGG 0.610618 7.2:-83599630 None:intergenic
TTGACATACCAAGGATACCA+TGG 0.612427 7.2:-83601107 None:intergenic
TATACCACATGAGGAAGGTG+GGG 0.612630 7.2:-83599628 None:intergenic
ATTCAGTGGGGATGTAGACT+CGG 0.622629 7.2:+83601025 MS.gene069416:intron
GGTGGGGCAACCGAATAGTG+TGG 0.623242 7.2:-83599612 None:intergenic
GGTACCGGACGAGCTGCCGT+CGG 0.623859 7.2:-83599587 None:intergenic
GGGGCAACCGAATAGTGTGG+CGG 0.631887 7.2:-83599609 None:intergenic
GGAAGTATACCACATGAGGA+AGG 0.632081 7.2:-83599633 None:intergenic
CATAGGAAGTATACCACATG+AGG 0.632795 7.2:-83599637 None:intergenic
CCTCCATGTAACAATTCCGA+CGG 0.638562 7.2:+83599571 MS.gene069416:CDS
AGTTGGCTACTAAAATACCA+TGG 0.682245 7.2:+83601090 MS.gene069416:CDS
CAGTGGGGATGTAGACTCGG+TGG 0.685981 7.2:+83601028 MS.gene069416:intron
CCGTCGGAATTGTTACATGG+AGG 0.692948 7.2:-83599571 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTATTTTTAATTT+CGG + chr7.2:83600785-83600804 MS.gene069416:intron 0.0%
!! GAATAAAAAAAAATATATGT+TGG + chr7.2:83599935-83599954 MS.gene069416:intron 10.0%
!! TATAAGATTAATTTATCTAA+TGG + chr7.2:83600203-83600222 MS.gene069416:intron 10.0%
!!! AAATATTTTTTGTTTTACTT+AGG + chr7.2:83600226-83600245 MS.gene069416:intron 10.0%
!!! AACTAGAAAAATTTAAAATT+AGG + chr7.2:83600720-83600739 MS.gene069416:intron 10.0%
!!! TTTTTTTTTTTTTTTTGTGA+AGG + chr7.2:83601848-83601867 MS.gene069416:intron 10.0%
!! AAGTAATTGAGATTTATTTA+AGG - chr7.2:83599973-83599992 None:intergenic 15.0%
!! ACAATTAATGGTAAAATAAA+AGG - chr7.2:83599832-83599851 None:intergenic 15.0%
!! AGATTTCATAAAACAATTAA+TGG - chr7.2:83599844-83599863 None:intergenic 15.0%
!!! AATAACAATCACTTTTTTAT+AGG - chr7.2:83601511-83601530 None:intergenic 15.0%
!!! ATTCTATAATTCAGTTTTTT+AGG + chr7.2:83601594-83601613 MS.gene069416:intron 15.0%
!! ATGTCATTAAAACAATTGTT+TGG + chr7.2:83601332-83601351 MS.gene069416:intron 20.0%
!! CAAGTTTATCAAAAATCTAT+TGG + chr7.2:83601636-83601655 MS.gene069416:intron 20.0%
!! GAATGATTTGAATAATTGAA+GGG - chr7.2:83601964-83601983 None:intergenic 20.0%
!! TATGCAAATTAAAGATTTGT+TGG + chr7.2:83600436-83600455 MS.gene069416:intron 20.0%
!! TTTAATTGCAAATTATCGTA+GGG - chr7.2:83601173-83601192 None:intergenic 20.0%
!! TTTACATAAATCCTAGAATT+CGG - chr7.2:83601742-83601761 None:intergenic 20.0%
!!! ACACTATAAAAGTTGAATTT+TGG - chr7.2:83600905-83600924 None:intergenic 20.0%
!!! ATTTTATCTGAAAATGAGTT+AGG + chr7.2:83601664-83601683 MS.gene069416:intron 20.0%
!!! TACACATGATTTGATAATAA+GGG - chr7.2:83600146-83600165 None:intergenic 20.0%
!!! TATGTCACAAAATTTTTACA+AGG + chr7.2:83600169-83600188 MS.gene069416:intron 20.0%
!!! TTACACATGATTTGATAATA+AGG - chr7.2:83600147-83600166 None:intergenic 20.0%
!!! TTTTAATTGCAAATTATCGT+AGG - chr7.2:83601174-83601193 None:intergenic 20.0%
!!! TTTTTTTTTTTTGTGAAGGT+TGG + chr7.2:83601852-83601871 MS.gene069416:intron 20.0%
! GATTAGCACATTATTTAGTA+TGG + chr7.2:83600059-83600078 MS.gene069416:intron 25.0%
! GGAATGATTTGAATAATTGA+AGG - chr7.2:83601965-83601984 None:intergenic 25.0%
! GTTCTACATTGAATAAGTTA+AGG + chr7.2:83601538-83601557 MS.gene069416:intron 25.0%
! TACATTGAATAAGTTAAGGT+TGG + chr7.2:83601542-83601561 MS.gene069416:intron 25.0%
! TATGTTTGTGTTAATTCAGT+GGG + chr7.2:83601012-83601031 MS.gene069416:intron 25.0%
! TTAATTGCAAATTATCGTAG+GGG - chr7.2:83601172-83601191 None:intergenic 25.0%
! TTATGTTTGTGTTAATTCAG+TGG + chr7.2:83601011-83601030 MS.gene069416:intron 25.0%
!! AAGGATTTTGCAATAGAATT+AGG + chr7.2:83599775-83599794 MS.gene069416:intron 25.0%
!! CTTTGAAGATCATTTCAATT+AGG - chr7.2:83600666-83600685 None:intergenic 25.0%
!! GATTTTTGATAAACTTGTGA+TGG - chr7.2:83601633-83601652 None:intergenic 25.0%
!!! AATTGTTTTAATGACATACG+AGG - chr7.2:83601329-83601348 None:intergenic 25.0%
!!! ATTGTTTTAATGACATACGA+GGG - chr7.2:83601328-83601347 None:intergenic 25.0%
!!! ATTTTGGACCCATTTTTTTT+TGG - chr7.2:83600889-83600908 None:intergenic 25.0%
!!! CTAAAAAAGTTTTAAGCAAG+AGG + chr7.2:83601464-83601483 MS.gene069416:intron 25.0%
!!! TACCTTAACTTGCTAATAAA+TGG - chr7.2:83600957-83600976 None:intergenic 25.0%
!!! TTTGTTTTACTTAGGCTTTT+AGG + chr7.2:83600234-83600253 MS.gene069416:intron 25.0%
AGATATTGAATAGAACGACA+TGG + chr7.2:83599905-83599924 MS.gene069416:intron 30.0%
AGTATTTCTTCACTTTCGTT+TGG + chr7.2:83601242-83601261 MS.gene069416:intron 30.0%
ATAAATCCTAGAATTCGGAT+TGG - chr7.2:83601737-83601756 None:intergenic 30.0%
ATCTTTATATAGCTATGCCA+TGG + chr7.2:83599503-83599522 MS.gene069416:CDS 30.0%
ATGCAGAAGTTTACTACAAT+AGG + chr7.2:83600291-83600310 MS.gene069416:CDS 30.0%
ATGTCCTAAGTAGTTTACTA+GGG + chr7.2:83601423-83601442 MS.gene069416:intron 30.0%
ATGTTTGTGTTAATTCAGTG+GGG + chr7.2:83601013-83601032 MS.gene069416:intron 30.0%
ATTAAACTCTTAGCTCAAAC+CGG + chr7.2:83601289-83601308 MS.gene069416:intron 30.0%
CAACCACTAATTGCTAAAAT+TGG - chr7.2:83601218-83601237 None:intergenic 30.0%
CCGACAAACATAACATAATA+CGG - chr7.2:83599665-83599684 None:intergenic 30.0%
CCGTATTATGTTATGTTTGT+CGG + chr7.2:83599662-83599681 MS.gene069416:intron 30.0%
CGACAAACATAACATAATAC+GGG - chr7.2:83599664-83599683 None:intergenic 30.0%
CGTATTATGTTATGTTTGTC+GGG + chr7.2:83599663-83599682 MS.gene069416:intron 30.0%
CTCCATTTATTAGCAAGTTA+AGG + chr7.2:83600952-83600971 MS.gene069416:intron 30.0%
GCTATCAATTCTCTATAAAG+TGG - chr7.2:83600544-83600563 None:intergenic 30.0%
TATGTCCTAAGTAGTTTACT+AGG + chr7.2:83601422-83601441 MS.gene069416:intron 30.0%
TGACATGGCCAAAAAAAAAT+GGG + chr7.2:83600878-83600897 MS.gene069416:intron 30.0%
TTATCTGAAAATGAGTTAGG+AGG + chr7.2:83601667-83601686 MS.gene069416:intron 30.0%
TTATTCAAATCATTCCTTGC+AGG + chr7.2:83601969-83601988 MS.gene069416:intron 30.0%
TTGACATGGCCAAAAAAAAA+TGG + chr7.2:83600877-83600896 MS.gene069416:intron 30.0%
TTTAATGAGATGAAGATCTC+AGG - chr7.2:83601275-83601294 None:intergenic 30.0%
! AAGTTTTCTGATTACTTTGC+TGG + chr7.2:83600683-83600702 MS.gene069416:intron 30.0%
! GATTTGATAATAAGGGCAAA+CGG - chr7.2:83600139-83600158 None:intergenic 30.0%
! TATAGAGAATTGATAGCTCA+TGG + chr7.2:83600547-83600566 MS.gene069416:CDS 30.0%
! TCTGCATATTTTTCTGTACA+TGG - chr7.2:83600278-83600297 None:intergenic 30.0%
!! CAATTTTAGCAATTAGTGGT+TGG + chr7.2:83601216-83601235 MS.gene069416:intron 30.0%
!!! GTGAAGTTTTGAATAGAAAC+TGG + chr7.2:83600494-83600513 MS.gene069416:intron 30.0%
!!! TTTTTTTTTGTGAAGGTTGG+AGG + chr7.2:83601855-83601874 MS.gene069416:intron 30.0%
ACATCTGAATACCTCTACTT+AGG - chr7.2:83600847-83600866 None:intergenic 35.0%
AGTTGGCTACTAAAATACCA+TGG + chr7.2:83601090-83601109 MS.gene069416:CDS 35.0%
ATGTTTGAAGGTCTTTGACA+TGG + chr7.2:83600863-83600882 MS.gene069416:intron 35.0%
CATAACATAATACGGGACAT+AGG - chr7.2:83599657-83599676 None:intergenic 35.0%
CTAAAATACCATGGTATCCT+TGG + chr7.2:83601099-83601118 MS.gene069416:CDS 35.0%
CTCTCTTTGTTCCAAAACTT+TGG + chr7.2:83599746-83599765 MS.gene069416:intron 35.0%
GTTACCAAATTCTTGATCCA+TGG - chr7.2:83599523-83599542 None:intergenic 35.0%
TATGCCATGGATCAAGAATT+TGG + chr7.2:83599516-83599535 MS.gene069416:CDS 35.0%
! CAAGGTATTCCTGCTTATAA+CGG - chr7.2:83600013-83600032 None:intergenic 35.0%
! GAGTAAGAGTTTGGATTTTC+AGG + chr7.2:83600569-83600588 MS.gene069416:CDS 35.0%
! TACTTAGGCTTTTAGGCAAA+TGG + chr7.2:83600241-83600260 MS.gene069416:intron 35.0%
! TGGAAACTTGATTTTGCTTG+TGG + chr7.2:83601352-83601371 MS.gene069416:intron 35.0%
!! AGAGGTATTCAGATGTTTGA+AGG + chr7.2:83600851-83600870 MS.gene069416:intron 35.0%
!! CGACCAATTTTAGCAATTAG+TGG + chr7.2:83601212-83601231 MS.gene069416:intron 35.0%
!! TGAAGTGCTTTTTGAACATC+TGG - chr7.2:83600317-83600336 None:intergenic 35.0%
!! TTACTAGGGTGCTGAATTAA+AGG + chr7.2:83601437-83601456 MS.gene069416:intron 35.0%
!!! AAGAGTTTGGATTTTCAGGT+TGG + chr7.2:83600573-83600592 MS.gene069416:intron 35.0%
AGATAGTGTAGCACATGATG+TGG + chr7.2:83601382-83601401 MS.gene069416:intron 40.0%
AGCACCCTAGTAAACTACTT+AGG - chr7.2:83601430-83601449 None:intergenic 40.0%
ATCATTGTTCTCGAGGTGTA+AGG - chr7.2:83600042-83600061 None:intergenic 40.0%
CAAATTATCGTAGGGGTCAA+GGG - chr7.2:83601165-83601184 None:intergenic 40.0%
CAACAGGCATTCCTTATAAG+TGG + chr7.2:83600345-83600364 MS.gene069416:CDS 40.0%
CGAGGTGTAAGGTAAAATCA+AGG - chr7.2:83600031-83600050 None:intergenic 40.0%
GCATATCTTGTTGCTGTAAC+TGG - chr7.2:83601056-83601075 None:intergenic 40.0%
TAGCTCATGGAGTAAGAGTT+TGG + chr7.2:83600560-83600579 MS.gene069416:CDS 40.0%
TCCTTGGTATGTCAAAAAGC+AGG + chr7.2:83601115-83601134 MS.gene069416:CDS 40.0%
TGTGCTAATCATTGTTCTCG+AGG - chr7.2:83600049-83600068 None:intergenic 40.0%
TTAGGTCCAATCCGAATTCT+AGG + chr7.2:83601728-83601747 MS.gene069416:intron 40.0%
TTGAATAGAACGACATGGTG+AGG + chr7.2:83599910-83599929 MS.gene069416:intron 40.0%
TTGACATACCAAGGATACCA+TGG - chr7.2:83601110-83601129 None:intergenic 40.0%
! CATAGGAAGTATACCACATG+AGG - chr7.2:83599640-83599659 None:intergenic 40.0%
! TCCAAAACTTTGGTAGCTGA+AGG + chr7.2:83599756-83599775 MS.gene069416:intron 40.0%
! TCCTTCAGCTACCAAAGTTT+TGG - chr7.2:83599760-83599779 None:intergenic 40.0%
! TTTTTGTGAAGGTTGGAGGT+TGG + chr7.2:83601859-83601878 MS.gene069416:intron 40.0%
!! CTCTACAGGCTGATTTTGTA+CGG + chr7.2:83601699-83601718 MS.gene069416:intron 40.0%
!! GATTTTGTACGGATCGACTT+AGG + chr7.2:83601710-83601729 MS.gene069416:intron 40.0%
!!! ACCTGCTTTTTGACATACCA+AGG - chr7.2:83601119-83601138 None:intergenic 40.0%
AAAATCAGCCTGTAGAGCGA+GGG - chr7.2:83601696-83601715 None:intergenic 45.0%
AAGTCCCACTATTCAAGCCT+AGG + chr7.2:83601934-83601953 MS.gene069416:intron 45.0%
AATAATTGAAGGGCTGCCCT+AGG - chr7.2:83601954-83601973 None:intergenic 45.0%
AGCCAACTTAAGCTGTGCAA+GGG - chr7.2:83601078-83601097 None:intergenic 45.0%
AGTATACCACATGAGGAAGG+TGG - chr7.2:83599633-83599652 None:intergenic 45.0%
AGTCCCACTATTCAAGCCTA+GGG + chr7.2:83601935-83601954 MS.gene069416:intron 45.0%
ATTCAGTGGGGATGTAGACT+CGG + chr7.2:83601025-83601044 MS.gene069416:intron 45.0%
CCTCCATGTAACAATTCCGA+CGG + chr7.2:83599571-83599590 MS.gene069416:CDS 45.0%
CGAGGGTTGAATAACTCAAC+CGG - chr7.2:83601311-83601330 None:intergenic 45.0%
CTAACATTTGCCACAGTGAG+AGG + chr7.2:83601900-83601919 MS.gene069416:intron 45.0%
CTGCAAGCAGTCCACTTATA+AGG - chr7.2:83600359-83600378 None:intergenic 45.0%
GAAATTGCCGAGCTTGGTAA+CGG + chr7.2:83600811-83600830 MS.gene069416:intron 45.0%
GAAGCTTGTGACCTAAGTAG+AGG + chr7.2:83600833-83600852 MS.gene069416:intron 45.0%
GCAAATTATCGTAGGGGTCA+AGG - chr7.2:83601166-83601185 None:intergenic 45.0%
GCACTTCATGCTAACACAAC+AGG + chr7.2:83600329-83600348 MS.gene069416:CDS 45.0%
GTATACCACATGAGGAAGGT+GGG - chr7.2:83599632-83599651 None:intergenic 45.0%
GTTACCAAGCTCGGCAATTT+CGG - chr7.2:83600812-83600831 None:intergenic 45.0%
GTTGGACTGAGGTGTATGAA+GGG + chr7.2:83601877-83601896 MS.gene069416:intron 45.0%
TAGCCAACTTAAGCTGTGCA+AGG - chr7.2:83601079-83601098 None:intergenic 45.0%
TAGTCTCTGCCGTTATAAGC+AGG + chr7.2:83600001-83600020 MS.gene069416:intron 45.0%
TATACCACATGAGGAAGGTG+GGG - chr7.2:83599631-83599650 None:intergenic 45.0%
! GGAAGTATACCACATGAGGA+AGG - chr7.2:83599636-83599655 None:intergenic 45.0%
! TAGGCTTTTAGGCAAATGGC+TGG + chr7.2:83600245-83600264 MS.gene069416:intron 45.0%
!! CTTATAAGTGGACTGCTTGC+AGG + chr7.2:83600357-83600376 MS.gene069416:CDS 45.0%
!! AAAAAAAAAAAAAAAAAGAA+AGG - chr7.2:83601844-83601863 None:intergenic 5.0%
!! AATAAAAAAAAATATATGTT+GGG + chr7.2:83599936-83599955 MS.gene069416:intron 5.0%
AATAAGGGCAAACGGCAACC+CGG - chr7.2:83600131-83600150 None:intergenic 50.0%
CAAAATCAGCCTGTAGAGCG+AGG - chr7.2:83601697-83601716 None:intergenic 50.0%
CAAGCTTCCGTTACCAAGCT+CGG - chr7.2:83600821-83600840 None:intergenic 50.0%
CCGTCGGAATTGTTACATGG+AGG - chr7.2:83599574-83599593 None:intergenic 50.0%
CTGCCCTAGGCTTGAATAGT+GGG - chr7.2:83601941-83601960 None:intergenic 50.0%
CTGCCGTCGGAATTGTTACA+TGG - chr7.2:83599577-83599596 None:intergenic 50.0%
GGTTGGACTGAGGTGTATGA+AGG + chr7.2:83601876-83601895 MS.gene069416:intron 50.0%
TGCCCTTGCACAGCTTAAGT+TGG + chr7.2:83601073-83601092 MS.gene069416:CDS 50.0%
TTGGAAGAGGCTTTCCTGCA+AGG - chr7.2:83601986-83602005 None:intergenic 50.0%
! TATGGAGCATTACCCCTTCC+CGG + chr7.2:83600077-83600096 MS.gene069416:intron 50.0%
ATGTCCTGCTCCTCTCACTG+TGG - chr7.2:83601913-83601932 None:intergenic 55.0%
GAAGGTTGGAGGTTGGACTG+AGG + chr7.2:83601866-83601885 MS.gene069416:intron 55.0%
GCTGCCCTAGGCTTGAATAG+TGG - chr7.2:83601942-83601961 None:intergenic 55.0%
TTTGCCACAGTGAGAGGAGC+AGG + chr7.2:83601906-83601925 MS.gene069416:intron 55.0%
AATTCCGACGGCAGCTCGTC+CGG + chr7.2:83599583-83599602 MS.gene069416:CDS 60.0%
ACTGAAGCTCCCGCATACGC+AGG - chr7.2:83600105-83600124 None:intergenic 60.0%
ATGCGGGAGCTTCAGTGCAC+CGG + chr7.2:83600109-83600128 MS.gene069416:intron 60.0%
CAGTGGGGATGTAGACTCGG+TGG + chr7.2:83601028-83601047 MS.gene069416:intron 60.0%
CCGGTACCCGCCACACTATT+CGG + chr7.2:83599602-83599621 MS.gene069416:CDS 60.0%
CGGTCCGAAATTGCCGAGCT+TGG + chr7.2:83600805-83600824 MS.gene069416:intron 60.0%
CTGAAGCTCCCGCATACGCA+GGG - chr7.2:83600104-83600123 None:intergenic 60.0%
GGGCAACCGAATAGTGTGGC+GGG - chr7.2:83599611-83599630 None:intergenic 60.0%
GGGGCAACCGAATAGTGTGG+CGG - chr7.2:83599612-83599631 None:intergenic 60.0%
GGTGGGGCAACCGAATAGTG+TGG - chr7.2:83599615-83599634 None:intergenic 60.0%
GTTGCCCCACCTTCCTCATG+TGG + chr7.2:83599624-83599643 MS.gene069416:CDS 60.0%
!! CCGAATAGTGTGGCGGGTAC+CGG - chr7.2:83599605-83599624 None:intergenic 60.0%
CATACGCAGGGTCCGGGAAG+GGG - chr7.2:83600092-83600111 None:intergenic 65.0%
CTTCCCGGACCCTGCGTATG+CGG + chr7.2:83600092-83600111 MS.gene069416:intron 65.0%
GCATACGCAGGGTCCGGGAA+GGG - chr7.2:83600093-83600112 None:intergenic 65.0%
TGCGGGAGCTTCAGTGCACC+GGG + chr7.2:83600110-83600129 MS.gene069416:intron 65.0%
TTCCCGGACCCTGCGTATGC+GGG + chr7.2:83600093-83600112 MS.gene069416:intron 65.0%
!! GGAGGCATCCCTCGCTCTAC+AGG + chr7.2:83601685-83601704 MS.gene069416:intron 65.0%
CGCATACGCAGGGTCCGGGA+AGG - chr7.2:83600094-83600113 None:intergenic 70.0%
CTCCCGCATACGCAGGGTCC+GGG - chr7.2:83600098-83600117 None:intergenic 70.0%
GCTCCCGCATACGCAGGGTC+CGG - chr7.2:83600099-83600118 None:intergenic 70.0%
! GGTACCGGACGAGCTGCCGT+CGG - chr7.2:83599590-83599609 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 83599493 83602014 83599493 ID=MS.gene069416
chr7.2 mRNA 83599493 83602014 83599493 ID=MS.gene069416.t1;Parent=MS.gene069416
chr7.2 exon 83599493 83599645 83599493 ID=MS.gene069416.t1.exon1;Parent=MS.gene069416.t1
chr7.2 CDS 83599493 83599645 83599493 ID=cds.MS.gene069416.t1;Parent=MS.gene069416.t1
chr7.2 exon 83600248 83600378 83600248 ID=MS.gene069416.t1.exon2;Parent=MS.gene069416.t1
chr7.2 CDS 83600248 83600378 83600248 ID=cds.MS.gene069416.t1;Parent=MS.gene069416.t1
chr7.2 exon 83600495 83600590 83600495 ID=MS.gene069416.t1.exon3;Parent=MS.gene069416.t1
chr7.2 CDS 83600495 83600590 83600495 ID=cds.MS.gene069416.t1;Parent=MS.gene069416.t1
chr7.2 exon 83601031 83601136 83601031 ID=MS.gene069416.t1.exon4;Parent=MS.gene069416.t1
chr7.2 CDS 83601031 83601136 83601031 ID=cds.MS.gene069416.t1;Parent=MS.gene069416.t1
chr7.2 exon 83601991 83602014 83601991 ID=MS.gene069416.t1.exon5;Parent=MS.gene069416.t1
chr7.2 CDS 83601991 83602014 83601991 ID=cds.MS.gene069416.t1;Parent=MS.gene069416.t1
Gene Sequence

>MS.gene069416

ATGAATGTGAATCTTTATATAGCTATGCCATGGATCAAGAATTTGGTAACATTGATTGATCAGTATAATATCTATGCTCCTCCATGTAACAATTCCGACGGCAGCTCGTCCGGTACCCGCCACACTATTCGGTTGCCCCACCTTCCTCATGTGGTATACTTCCTATGTCCCGTATTATGTTATGTTTGTCGGGTCTTGAATTTGTTTGTCTCAAATTATTTGTCGTTTTAGAATATAATTGCAATTTTTGTCACTCTCTTTGTTCCAAAACTTTGGTAGCTGAAGGATTTTGCAATAGAATTAGGAAACATTTTTAGTTGATGCATATTTGTATCACCTTTTATTTTACCATTAATTGTTTTATGAAATCTCTTTTTTTTAAGAAAATAATATAAAATGCTAAATTGATCAAAGATATTGAATAGAACGACATGGTGAGGCAGAATAAAAAAAAATATATGTTGGGAGAAGTCGATACCTTAAATAAATCTCAATTACTTTAAATTATTAGTCTCTGCCGTTATAAGCAGGAATACCTTGATTTTACCTTACACCTCGAGAACAATGATTAGCACATTATTTAGTATGGAGCATTACCCCTTCCCGGACCCTGCGTATGCGGGAGCTTCAGTGCACCGGGTTGCCGTTTGCCCTTATTATCAAATCATGTGTAATATATGTCACAAAATTTTTACAAGGTTGTCTTATTATATAAGATTAATTTATCTAATGGAAATATTTTTTGTTTTACTTAGGCTTTTAGGCAAATGGCTGGTTATGATCCATGTACAGAAAAATATGCAGAAGTTTACTACAATAGGCCAGATGTTCAAAAAGCACTTCATGCTAACACAACAGGCATTCCTTATAAGTGGACTGCTTGCAGGTGATATTACTTTTGATGTCACAAGTTTAAGTTAAAAAAGTGATAATTATTTATTATTATGCAAATTAAAGATTTGTTGGTTGATAATTAATTAAGTTTCTTTTCATTTGATGCAGTGAAGTTTTGAATAGAAACTGGAATGATACAGATGTCTCTGTTCTTCCACTTTATAGAGAATTGATAGCTCATGGAGTAAGAGTTTGGATTTTCAGGTTGGTTTTGAAATATGCATTTATAACTTTCTTCTTTTTGTCTTTAGTACTATACACTAGAAAAAATTGATTCCTAATTGAAATGATCTTCAAAGTTTTCTGATTACTTTGCTGGTAAAAAAAAAAAAAAACTAGAAAAATTTAAAATTAGGAACAGAAAAATATATAATTGAAATTTAATTTGCAAATTTCATTTTTTTTTTATTTTTAATTTCGGTCCGAAATTGCCGAGCTTGGTAACGGAAGCTTGTGACCTAAGTAGAGGTATTCAGATGTTTGAAGGTCTTTGACATGGCCAAAAAAAAATGGGTCCAAAATTCAACTTTTATAGTGTATGTGAAAAATTATAAATAAAATTGTACTCCATTTATTAGCAAGTTAAGGTATTATTTAATTAGTATGATAGCATATACATATTAATTATGTTTGTGTTAATTCAGTGGGGATGTAGACTCGGTGGTTCCAGTTACAGCAACAAGATATGCCCTTGCACAGCTTAAGTTGGCTACTAAAATACCATGGTATCCTTGGTATGTCAAAAAGCAGGTGAGTTCAATTCAATTTTGTTCTTCCCTTGACCCCTACGATAATTTGCAATTAAAACAATTTTTTTGTTTGAAACGACCAATTTTAGCAATTAGTGGTTGGATTAGTATTTCTTCACTTTCGTTTGGACTTGAACCTGAGATCTTCATCTCATTAAACTCTTAGCTCAAACCGGTTGAGTTATTCAACCCTCGTATGTCATTAAAACAATTGTTTGGAAACTTGATTTTGCTTGTGGATTTAATAGATAGTGTAGCACATGATGTGGATTTAAGTGAACATTTATATGTCCTAAGTAGTTTACTAGGGTGCTGAATTAAAGGACATCTAAAAAAGTTTTAAGCAAGAGGTTCATGATTACTTAACATTTGCCTATAAAAAAGTGATTGTTATTGTTTAGAGTTCTACATTGAATAAGTTAAGGTTGGAGATGAGTTTTAAGTCAGAGTCTTCTCTCATTCTATAATTCAGTTTTTTAGGAGTAGAGTTTGATCCATCACAAGTTTATCAAAAATCTATTGGATCACATTTTATCTGAAAATGAGTTAGGAGGCATCCCTCGCTCTACAGGCTGATTTTGTACGGATCGACTTAGGTCCAATCCGAATTCTAGGATTTATGTAAATGAATAATCATTTCACATCTTAAAAGTATATAAATTGATTCAGTTGAGTTTTACAAGTATAGACACACTAAGCTAATTTCCTTTCTTTTTTTTTTTTTTTTTGTGAAGGTTGGAGGTTGGACTGAGGTGTATGAAGGGCTAACATTTGCCACAGTGAGAGGAGCAGGACATGAAGTCCCACTATTCAAGCCTAGGGCAGCCCTTCAATTATTCAAATCATTCCTTGCAGGAAAGCCTCTTCCAAAAGTCCTAA
Protein sequence

>MS.gene069416.t1

MNVNLYIAMPWIKNLVTLIDQYNIYAPPCNNSDGSSSGTRHTIRLPHLPHVAFRQMAGYDPCTEKYAEVYYNRPDVQKALHANTTGIPYKWTACSEVLNRNWNDTDVSVLPLYRELIAHGVRVWIFSGDVDSVVPVTATRYALAQLKLATKIPWYPWYVKKQESLFQKS