Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069444.t1 | XP_003620175.1 | 93.5 | 214 | 13 | 1 | 1 | 214 | 1 | 213 | 8.10E-113 | 416.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069444.t1 | P13087 | 45.0 | 211 | 102 | 5 | 8 | 213 | 15 | 216 | 4.1e-41 | 169.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069444.t1 | Q6ISX8 | 93.5 | 214 | 13 | 1 | 1 | 214 | 1 | 213 | 5.8e-113 | 416.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05227 | MS.gene069444 | 0.854008 | 1.64E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069444.t1 | MTR_6g078140 | 93.458 | 214 | 13 | 1 | 1 | 214 | 1 | 213 | 4.01e-136 | 380 |
| MS.gene069444.t1 | MTR_6g078120 | 92.991 | 214 | 14 | 1 | 1 | 214 | 1 | 213 | 4.15e-135 | 377 |
| MS.gene069444.t1 | MTR_6g078280 | 60.500 | 200 | 72 | 4 | 18 | 214 | 17 | 212 | 1.90e-80 | 239 |
| MS.gene069444.t1 | MTR_6g078290 | 58.140 | 215 | 86 | 4 | 1 | 214 | 1 | 212 | 3.24e-80 | 239 |
| MS.gene069444.t1 | MTR_6g078250 | 56.122 | 196 | 77 | 2 | 16 | 211 | 15 | 201 | 9.11e-72 | 217 |
| MS.gene069444.t1 | MTR_6g078260 | 47.706 | 218 | 91 | 6 | 1 | 214 | 1 | 199 | 5.43e-57 | 179 |
| MS.gene069444.t1 | MTR_7g037410 | 49.198 | 187 | 83 | 4 | 29 | 214 | 27 | 202 | 4.59e-56 | 177 |
| MS.gene069444.t1 | MTR_6g078070 | 40.359 | 223 | 104 | 10 | 1 | 214 | 1 | 203 | 9.07e-40 | 135 |
| MS.gene069444.t1 | MTR_6g078100 | 39.823 | 226 | 103 | 11 | 1 | 214 | 1 | 205 | 1.66e-38 | 132 |
| MS.gene069444.t1 | MTR_6g065460 | 31.797 | 217 | 132 | 7 | 1 | 214 | 1 | 204 | 2.17e-19 | 82.8 |
| MS.gene069444.t1 | MTR_3g106620 | 29.493 | 217 | 121 | 7 | 9 | 214 | 4 | 199 | 2.24e-19 | 82.8 |
| MS.gene069444.t1 | MTR_3g106585 | 31.364 | 220 | 111 | 9 | 9 | 214 | 4 | 197 | 1.93e-17 | 77.8 |
| MS.gene069444.t1 | MTR_2g073010 | 32.589 | 224 | 115 | 11 | 1 | 214 | 1 | 198 | 5.73e-16 | 73.6 |
| MS.gene069444.t1 | MTR_8g461050 | 29.384 | 211 | 127 | 8 | 10 | 213 | 6 | 201 | 4.12e-14 | 68.6 |
| MS.gene069444.t1 | MTR_6g059650 | 30.612 | 196 | 111 | 11 | 26 | 213 | 23 | 201 | 6.53e-14 | 68.2 |
| MS.gene069444.t1 | MTR_6g017225 | 31.395 | 172 | 102 | 6 | 4 | 174 | 6 | 162 | 1.06e-13 | 69.3 |
| MS.gene069444.t1 | MTR_2g072980 | 31.280 | 211 | 118 | 10 | 8 | 214 | 12 | 199 | 1.08e-13 | 67.4 |
| MS.gene069444.t1 | MTR_6g078040 | 47.436 | 78 | 36 | 4 | 104 | 179 | 1 | 75 | 5.88e-12 | 60.1 |
| MS.gene069444.t1 | MTR_6g009650 | 30.435 | 207 | 119 | 8 | 4 | 205 | 6 | 192 | 8.37e-12 | 62.4 |
| MS.gene069444.t1 | MTR_8g059790 | 28.421 | 190 | 114 | 8 | 31 | 213 | 29 | 203 | 1.21e-11 | 62.0 |
| MS.gene069444.t1 | MTR_6g059410 | 28.507 | 221 | 124 | 11 | 1 | 214 | 1 | 194 | 2.24e-11 | 61.2 |
| MS.gene069444.t1 | MTR_6g009563 | 29.767 | 215 | 125 | 9 | 6 | 214 | 9 | 203 | 2.57e-11 | 61.2 |
| MS.gene069444.t1 | MTR_1g035770 | 27.488 | 211 | 124 | 8 | 4 | 203 | 6 | 198 | 5.19e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069444.t1 | AT1G17860 | 43.119 | 218 | 97 | 8 | 1 | 214 | 1 | 195 | 3.64e-47 | 154 |
| MS.gene069444.t1 | AT1G73260 | 36.000 | 200 | 110 | 6 | 21 | 214 | 18 | 205 | 1.44e-28 | 107 |
| MS.gene069444.t1 | AT1G73325 | 34.673 | 199 | 107 | 9 | 22 | 214 | 22 | 203 | 1.43e-23 | 94.4 |
| MS.gene069444.t1 | AT1G73330 | 28.378 | 222 | 121 | 13 | 1 | 214 | 1 | 192 | 4.17e-11 | 60.5 |
Find 52 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATCTTGAACCAATTATTAA+TGG | 0.190104 | 6.4:-52740085 | None:intergenic |
| ACACGAATAACACCCTTCTT+AGG | 0.207105 | 6.4:-52739919 | None:intergenic |
| TTGAGCACACAACCACTTCT+TGG | 0.247520 | 6.4:+52739664 | MS.gene069444:CDS |
| GATGGGAAACCATTAATAAT+TGG | 0.274442 | 6.4:+52740076 | MS.gene069444:CDS |
| GGAGTGTTTGTTGATGAAAA+TGG | 0.277827 | 6.4:+52740186 | MS.gene069444:CDS |
| CACCTGTGAATGGGTATAAC+CGG | 0.336556 | 6.4:-52739766 | None:intergenic |
| AGACAATGGTTTATTACTAT+TGG | 0.339490 | 6.4:+52740033 | MS.gene069444:CDS |
| TGTTGAAATTTAACTTTAAG+AGG | 0.341900 | 6.4:-52740237 | None:intergenic |
| ACACCCTTCTTAGGGTTAAC+AGG | 0.353495 | 6.4:-52739910 | None:intergenic |
| ATTCACAGGTGTGGACCTTA+TGG | 0.370571 | 6.4:+52739778 | MS.gene069444:CDS |
| TGAAAATGGGAATAAACGTT+TGG | 0.383026 | 6.4:+52740200 | MS.gene069444:CDS |
| TGGTTCAAGATTGAGAAGTA+TGG | 0.396705 | 6.4:+52740096 | MS.gene069444:CDS |
| TGGTGGTGTTGTTGGAAATC+CGG | 0.404788 | 6.4:+52740053 | MS.gene069444:CDS |
| GAGTGTTTGTTGATGAAAAT+GGG | 0.410318 | 6.4:+52740187 | MS.gene069444:CDS |
| ATTACTATTGGTGGTGTTGT+TGG | 0.423355 | 6.4:+52740045 | MS.gene069444:CDS |
| GAACAAGTGGTTGACACATT+AGG | 0.434007 | 6.4:+52739706 | MS.gene069444:CDS |
| TATGCCTGTTAACCCTAAGA+AGG | 0.446168 | 6.4:+52739906 | MS.gene069444:CDS |
| CACGAATAACACCCTTCTTA+GGG | 0.454923 | 6.4:-52739918 | None:intergenic |
| TTGAAAGACTGCACCACACT+AGG | 0.459638 | 6.4:-52740144 | None:intergenic |
| GTACTTCTACATTTACCATA+AGG | 0.462600 | 6.4:-52739793 | None:intergenic |
| AGTCTTGCCCTAGCAAGTAT+TGG | 0.463122 | 6.4:+52739823 | MS.gene069444:CDS |
| GTCTTTCAAGCATATGTGTA+AGG | 0.466167 | 6.4:+52740158 | MS.gene069444:CDS |
| AACTTGCCCTCTTGATGTTG+TGG | 0.473079 | 6.4:+52739849 | MS.gene069444:CDS |
| CAATGGTTTATTACTATTGG+TGG | 0.475905 | 6.4:+52740036 | MS.gene069444:CDS |
| TATGGTAAATGTAGAAGTAC+TGG | 0.482361 | 6.4:+52739796 | MS.gene069444:CDS |
| TTATTAATGGTTTCCCATCC+CGG | 0.501459 | 6.4:-52740072 | None:intergenic |
| AAGCATATGTGTAAGGATGT+TGG | 0.504444 | 6.4:+52740165 | MS.gene069444:CDS |
| ATAAGGTCCACACCTGTGAA+TGG | 0.514083 | 6.4:-52739776 | None:intergenic |
| TAAGGTCCACACCTGTGAAT+GGG | 0.519817 | 6.4:-52739775 | None:intergenic |
| TGCTAATTACTATATTATCC+CGG | 0.540548 | 6.4:+52739747 | MS.gene069444:CDS |
| GGTGGTGTTGTTGGAAATCC+GGG | 0.542963 | 6.4:+52740054 | MS.gene069444:CDS |
| GCTTCAGCTGCTCCAAGAAG+TGG | 0.551993 | 6.4:-52739676 | None:intergenic |
| TCCCGGTTATACCCATTCAC+AGG | 0.554778 | 6.4:+52739764 | MS.gene069444:CDS |
| ATGCCTGTTAACCCTAAGAA+GGG | 0.557642 | 6.4:+52739907 | MS.gene069444:CDS |
| GTGTTGTTGGAAATCCGGGA+TGG | 0.564391 | 6.4:+52740058 | MS.gene069444:CDS |
| TGTTGTTGGAAATCCGGGAT+GGG | 0.569448 | 6.4:+52740059 | MS.gene069444:CDS |
| TGAAAGACTGCACCACACTA+GGG | 0.577137 | 6.4:-52740143 | None:intergenic |
| TTAACAGGCATAAAAGTAAG+TGG | 0.579627 | 6.4:-52739895 | None:intergenic |
| CAAGCTTCCACACCATGGAA+TGG | 0.584005 | 6.4:-52739990 | None:intergenic |
| TGAAGCTTCAAATGAACAAG+TGG | 0.587694 | 6.4:+52739693 | MS.gene069444:CDS |
| AAGTGGTTGTGTGCTCAAGA+AGG | 0.588756 | 6.4:-52739659 | None:intergenic |
| GTCTACACCATTCCATGGTG+TGG | 0.596900 | 6.4:+52739983 | MS.gene069444:CDS |
| ACCTGTGAATGGGTATAACC+GGG | 0.598899 | 6.4:-52739765 | None:intergenic |
| AACTTGTCTACACCATTCCA+TGG | 0.601053 | 6.4:+52739978 | MS.gene069444:CDS |
| TTAATGTATCTAAGAGACAA+TGG | 0.603832 | 6.4:+52740019 | MS.gene069444:CDS |
| TCAACAACCACAACATCAAG+AGG | 0.610607 | 6.4:-52739856 | None:intergenic |
| TGCTCAAGAAGGCAAAGAGA+AGG | 0.611541 | 6.4:-52739648 | None:intergenic |
| GTATGGTGATGATTATAAGT+TGG | 0.617624 | 6.4:+52740113 | MS.gene069444:CDS |
| TATAGTAATTAGCATCAGCT+CGG | 0.621190 | 6.4:-52739738 | None:intergenic |
| GTTATACCCATTCACAGGTG+TGG | 0.621916 | 6.4:+52739769 | MS.gene069444:CDS |
| ACGATCAAGCTTCCACACCA+TGG | 0.629046 | 6.4:-52739995 | None:intergenic |
| CAACAACCACAACATCAAGA+GGG | 0.678487 | 6.4:-52739855 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCTTGAACCAATTATTAA+TGG | - | chr6.4:52740088-52740107 | None:intergenic | 20.0% |
| !!! | TGTTGAAATTTAACTTTAAG+AGG | - | chr6.4:52740240-52740259 | None:intergenic | 20.0% |
| ! | AGACAATGGTTTATTACTAT+TGG | + | chr6.4:52740033-52740052 | MS.gene069444:CDS | 25.0% |
| ! | TGCTAATTACTATATTATCC+CGG | + | chr6.4:52739747-52739766 | MS.gene069444:CDS | 25.0% |
| ! | TTAATGTATCTAAGAGACAA+TGG | + | chr6.4:52740019-52740038 | MS.gene069444:CDS | 25.0% |
| CAATGGTTTATTACTATTGG+TGG | + | chr6.4:52740036-52740055 | MS.gene069444:CDS | 30.0% | |
| GATGGGAAACCATTAATAAT+TGG | + | chr6.4:52740076-52740095 | MS.gene069444:CDS | 30.0% | |
| GTACTTCTACATTTACCATA+AGG | - | chr6.4:52739796-52739815 | None:intergenic | 30.0% | |
| TATAGTAATTAGCATCAGCT+CGG | - | chr6.4:52739741-52739760 | None:intergenic | 30.0% | |
| TGAAAATGGGAATAAACGTT+TGG | + | chr6.4:52740200-52740219 | MS.gene069444:CDS | 30.0% | |
| TTAACAGGCATAAAAGTAAG+TGG | - | chr6.4:52739898-52739917 | None:intergenic | 30.0% | |
| ! | AAGTTTTTCCAATACTTGCT+AGG | - | chr6.4:52739834-52739853 | None:intergenic | 30.0% |
| ! | GAGTGTTTGTTGATGAAAAT+GGG | + | chr6.4:52740187-52740206 | MS.gene069444:CDS | 30.0% |
| ! | GTATGGTGATGATTATAAGT+TGG | + | chr6.4:52740113-52740132 | MS.gene069444:CDS | 30.0% |
| ! | TATGGTAAATGTAGAAGTAC+TGG | + | chr6.4:52739796-52739815 | MS.gene069444:CDS | 30.0% |
| !! | AGTTTTTCCAATACTTGCTA+GGG | - | chr6.4:52739833-52739852 | None:intergenic | 30.0% |
| AAGCATATGTGTAAGGATGT+TGG | + | chr6.4:52740165-52740184 | MS.gene069444:CDS | 35.0% | |
| GTCTTTCAAGCATATGTGTA+AGG | + | chr6.4:52740158-52740177 | MS.gene069444:CDS | 35.0% | |
| TGAAGCTTCAAATGAACAAG+TGG | + | chr6.4:52739693-52739712 | MS.gene069444:CDS | 35.0% | |
| TGGTTCAAGATTGAGAAGTA+TGG | + | chr6.4:52740096-52740115 | MS.gene069444:CDS | 35.0% | |
| TTATTAATGGTTTCCCATCC+CGG | - | chr6.4:52740075-52740094 | None:intergenic | 35.0% | |
| ! | GGAGTGTTTGTTGATGAAAA+TGG | + | chr6.4:52740186-52740205 | MS.gene069444:CDS | 35.0% |
| !! | ATTACTATTGGTGGTGTTGT+TGG | + | chr6.4:52740045-52740064 | MS.gene069444:CDS | 35.0% |
| AACTTGTCTACACCATTCCA+TGG | + | chr6.4:52739978-52739997 | MS.gene069444:CDS | 40.0% | |
| ACACGAATAACACCCTTCTT+AGG | - | chr6.4:52739922-52739941 | None:intergenic | 40.0% | |
| ATGCCTGTTAACCCTAAGAA+GGG | + | chr6.4:52739907-52739926 | MS.gene069444:CDS | 40.0% | |
| CAACAACCACAACATCAAGA+GGG | - | chr6.4:52739858-52739877 | None:intergenic | 40.0% | |
| CACGAATAACACCCTTCTTA+GGG | - | chr6.4:52739921-52739940 | None:intergenic | 40.0% | |
| GAACAAGTGGTTGACACATT+AGG | + | chr6.4:52739706-52739725 | MS.gene069444:CDS | 40.0% | |
| TATGCCTGTTAACCCTAAGA+AGG | + | chr6.4:52739906-52739925 | MS.gene069444:CDS | 40.0% | |
| TCAACAACCACAACATCAAG+AGG | - | chr6.4:52739859-52739878 | None:intergenic | 40.0% | |
| AACTTGCCCTCTTGATGTTG+TGG | + | chr6.4:52739849-52739868 | MS.gene069444:CDS | 45.0% | |
| AAGTGGTTGTGTGCTCAAGA+AGG | - | chr6.4:52739662-52739681 | None:intergenic | 45.0% | |
| ACACCCTTCTTAGGGTTAAC+AGG | - | chr6.4:52739913-52739932 | None:intergenic | 45.0% | |
| ACCTGTGAATGGGTATAACC+GGG | - | chr6.4:52739768-52739787 | None:intergenic | 45.0% | |
| AGTCTTGCCCTAGCAAGTAT+TGG | + | chr6.4:52739823-52739842 | MS.gene069444:CDS | 45.0% | |
| ATAAGGTCCACACCTGTGAA+TGG | - | chr6.4:52739779-52739798 | None:intergenic | 45.0% | |
| ATTCACAGGTGTGGACCTTA+TGG | + | chr6.4:52739778-52739797 | MS.gene069444:CDS | 45.0% | |
| CACCTGTGAATGGGTATAAC+CGG | - | chr6.4:52739769-52739788 | None:intergenic | 45.0% | |
| GTTATACCCATTCACAGGTG+TGG | + | chr6.4:52739769-52739788 | MS.gene069444:CDS | 45.0% | |
| TAAGGTCCACACCTGTGAAT+GGG | - | chr6.4:52739778-52739797 | None:intergenic | 45.0% | |
| TGAAAGACTGCACCACACTA+GGG | - | chr6.4:52740146-52740165 | None:intergenic | 45.0% | |
| TGCTCAAGAAGGCAAAGAGA+AGG | - | chr6.4:52739651-52739670 | None:intergenic | 45.0% | |
| TGTTGTTGGAAATCCGGGAT+GGG | + | chr6.4:52740059-52740078 | MS.gene069444:CDS | 45.0% | |
| TTGAAAGACTGCACCACACT+AGG | - | chr6.4:52740147-52740166 | None:intergenic | 45.0% | |
| TTGAGCACACAACCACTTCT+TGG | + | chr6.4:52739664-52739683 | MS.gene069444:CDS | 45.0% | |
| !! | TGGTGGTGTTGTTGGAAATC+CGG | + | chr6.4:52740053-52740072 | MS.gene069444:CDS | 45.0% |
| ACGATCAAGCTTCCACACCA+TGG | - | chr6.4:52739998-52740017 | None:intergenic | 50.0% | |
| CAAGCTTCCACACCATGGAA+TGG | - | chr6.4:52739993-52740012 | None:intergenic | 50.0% | |
| GTCTACACCATTCCATGGTG+TGG | + | chr6.4:52739983-52740002 | MS.gene069444:CDS | 50.0% | |
| TCCCGGTTATACCCATTCAC+AGG | + | chr6.4:52739764-52739783 | MS.gene069444:CDS | 50.0% | |
| ! | GTGTTGTTGGAAATCCGGGA+TGG | + | chr6.4:52740058-52740077 | MS.gene069444:CDS | 50.0% |
| !! | GGTGGTGTTGTTGGAAATCC+GGG | + | chr6.4:52740054-52740073 | MS.gene069444:CDS | 50.0% |
| !! | GTTGGTGTTTTGCCCTAGTG+TGG | + | chr6.4:52740131-52740150 | MS.gene069444:CDS | 50.0% |
| GCTTCAGCTGCTCCAAGAAG+TGG | - | chr6.4:52739679-52739698 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 52739622 | 52740266 | 52739622 | ID=MS.gene069444 |
| chr6.4 | mRNA | 52739622 | 52740266 | 52739622 | ID=MS.gene069444.t1;Parent=MS.gene069444 |
| chr6.4 | exon | 52739622 | 52740266 | 52739622 | ID=MS.gene069444.t1.exon1;Parent=MS.gene069444.t1 |
| chr6.4 | CDS | 52739622 | 52740266 | 52739622 | ID=cds.MS.gene069444.t1;Parent=MS.gene069444.t1 |
| Gene Sequence |
| Protein sequence |
